| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| Human cyclin dependent kinase ( CDK ) activating kinase ( CAK ) has a dual function in cross regulation of cell cycle and differentiation , whereas menage a trois 1 ( MAT 1 ) assembles CAK and determines CAK ' s substrate specificity . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| MAT 1 ( ' menage trois ' ) a new RING finger protein subunit stabilizing cyclin H cdk 7 complexes in starfish and Xenopus CAK . ^^^ We demonstrate that the RING finger protein ( MAT 1 : ' menage trois ' ) is a new subunit of CAK in both vertebrate and invertebrates . ^^^ Stable heterotrimeric CAK complexes were generated in reticulocyte lysates programmed with mRNAs encoding Xenopus cdk 7 , cyclin H and MAT 1 . ^^^ Stabilization of CAK complexes by MAT 1 does not involve phosphorylation of Thr 176 , as the Thr 176 > Ala mutant of Xenopus cdk 7 could engage as efficiently as wild type cdk 7 in ternary complexes . ^^^ Even though starfish MAT 1 is almost identical to Xenopus MAT 1 in the RING finger domain , the starfish subunit could not replace the Xenopus subunit and stabilize cyclin H cdk 7 in reticulocyte lysate , suggesting that the MAT 1 subunit does not ( or not only ) interact with cyclin H cdk 7 through the RING finger domain . . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| Molecular cloning of CDK 7 associated human MAT 1 , a cyclin dependent kinase activating kinase ( CAK ) assembly factor . ^^^ Through a large scale immunopurification of CDK 7 complexes and protein microsequencing , we have isolated a cDNA that encodes p 35 and have shown that it is the human homologue of Mat 1 that is involved in the assembly of CAK . ^^^ The human MAT 1 gene expresses a single 1 . 6 kb transcript , the steady state level of which , like CDK 7 and cyclin H , varies significantly in different cell lines and in different terminally differentiated tissues . . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| In vitro assembly of a functional human CDK 7 cyclin H complex requires MAT 1 , a novel 36 kDa RING finger protein . ^^^ Here we report that in vitro reconstitution of an active CDK 7 cyclin H complex requires stoichiometric amounts of a novel 36 kDa assembly factor termed MAT 1 ( mnage trois 1 ) . ^^^ Sequencing of MAT 1 reveals a putative zinc binding motif ( a C3HC4 RING finger ) in the N terminus ; however , this domain is not required for ternary complex formation with CDK 7 cyclin H . ^^^ MAT 1 is associated with nuclear CDK 7 cyclin H at all stages of the cell cycle in vivo . ^^^ Ternary complexes of CDK 7 , cyclin H and MAT 1 display kinase activity towards substrates mimicking both the T loop in CDKs and the C terminal domain of RNA polymerase 2 , regardless of whether they are immunoprecipitated from HeLa cells or reconstituted in a reticulocyte lysate . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| MAT 1 , cdk 7 and cyclin H form a kinase complex which is UV light sensitive upon association with TFIIH . ^^^ MAT 1 , cyclin H and cdk 7 are part of TFIIH , a class 2 transcription factor which possesses numerous subunits of which several have been shown to be involved in processes other than transcription . ^^^ Two of them , XPD ( ERCC 2 ) and XPB ( ERCC 3 ) , are helicases involved in nucleotide excision repair ( NER ) , whereas cdk 7 , cyclin H and MAT 1 are thought to participate in cell cycle regulation . ^^^ MAT 1 , cyclin H and cdk 7 exist as a ternary complex either free or associated with TFIIH from which the latter can be dissociated at high salt concentration . ^^^ MAT 1 is strongly associated with cdk 7 and cyclin H . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| The kinase activity was recently identified as the cdk ( cyclin dependent kinase ) activating kinase , CAK , composed of cdk 7 , cyclin H , and MAT 1 . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| In vertebrates , CAK is a trimeric enzyme containing CDK 7 , cyclin H , and MAT 1 . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| Finally , we show that dual phosphorylation of S 170 and T 176 , or substitution of both phosphorylatable residues by aspartic residues , is sufficient to generate CAK activity to one third of its maximal value in vitro , even in the absence of cyclin H and MAT 1 , and may thus provide further clues as to how cyclins activate cdk subunits . . ^^^ This enzyme exists in either of two forms : the major one contains cdk 7 , cyclin H and an assembly factor called MAT 1 , whilst the minor one lacks MAT 1 . ^^^ In the absence of MAT 1 , phosphorylation of the T loop appears to be required for cdk 7 to bind cyclin H . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| The structural homology is even higher for the residues at the interface with the kinase , which is of functional significance , as shown by our observation that cyclin H binds to cyclin dependent kinase 2 ( cdk 2 ) and that cyclin A is able to activate cdk 7 in the presence of MAT 1 . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| Three subunits of TFIIH , cdk 7 , cyclin H and MAT 1 , form a ternary complex , cdk activating kinase ( CAK ) , found either on its own or as part of TFIIH . ^^^ TFIIH was resolved into four subcomplexes : the kinase complex composed of cdk 7 , cyclin H and MAT 1 ; the core TFIIH which contains XPB , p 62 , p 52 , p 44 and p 34 ; and two other subcomplexes in which XPD is found associated with either the kinase complex or with the core TFIIH . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| Most CAK exists as complexes of three subunits : CDK 7 , cyclin H ( CycH ) and MAT 1 . ^^^ We suggest that the MAT 1 protein , previously shown to function as an assembly factor for CDK 7 CycH , also acts to modulate CAK substrate specificity . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| Both free CAK ( cdk 7 , cyclin H , MAT 1 ) and the CAK containing general transcription factor TFIIH phosphorylated Ser 77 in vitro . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| The cdk 7 cyclin H MAT 1 complex associated with TFIIH is localized in coiled bodies . ^^^ TFIIH is composed of eight or nine subunits and we show that at least four of them , namely cdk 7 , cyclin H , MAT 1 , and p 62 are localized in the coiled body , a distinct subnuclear structure that is transcription dependent and highly enriched in small nuclear ribonucleoproteins . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| Rig 2 is likely to be a homolog of MAT 1 of higher eukaryotes which forms a ternary complex with MO 15 ( cdk 7 ) and cyclin H . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| CAK was first purified biochemically from higher eukaryotes and identified as a trimeric complex containing a cdk 7 catalytic subunit , cyclin H and MAT 1 ( Mnage trois ) , a member of the RING finger family . ^^^ CAK was first purified biochemically from higher eukaryotes and identified as a trimeric complex containing a cdk 7 catalytic subunit , cyclin H and MAT 1 ( Mnage trois ) , a member of the RING finger family . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| The cyclin dependent kinase activating kinase ( CAK ) assembly factor , MAT 1 , targets and enhances CAK activity on the POU domains of octamer transcription factors . ^^^ MAT 1 is known to be required for the assembly of cyclin dependent kinase ( CDK ) activating kinase ( CAK ) , which is functionally associated with the general transcription factor IIH ( TFIIH ) . ^^^ MAT 1 is shown to enhance the ability of a recombinant CDK 7 cyclin H complex ( bipartite CAK ) to phosphorylate isolated POU domains , intact Oct 1 , and the C terminal domain of RNA polymerase 2 , but not the originally defined substrate , CDK 2 . ^^^ These results suggest ( 1 ) that interactions between POU domains and MAT 1 can target CAK to Oct factors and result in their phosphorylation , ( 2 ) that MAT 1 not only functions as a CAK assembly factor but also acts to alter the spectrum of CAK substrates , and ( 3 ) that a POU MAT 1 interaction may play a role in the recruitment of TFIIH to the preinitiation complex or in subsequent initiation and elongation reactions . . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| Mapping of the human genes encoding cyclin H ( CCNH ) and the CDK activating kinase ( CAK ) assembly factor MAT 1 ( MNAT 1 ) to chromosome bands 5q13 . 3 q 14 and 14q23 , respectively . ^^^ Mammalian CAK contains three components : CDK 7 , cyclin H , and an assembly factor called MAT 1 . ^^^ Here , we have determined chromosomal localizations of the human genes encoding cyclin H ( CCNH ) and MAT 1 ( HGMW approved symbol MNAT 1 ) to chromosome bands 5q13 . 3 q 14 and 14q23 , respectively , by using fluorescence in situ hybridization , somatic cell hybrid analyses , and mapping to the human YAC contigs . . ^^^ The CDK 7 cyclin H MAT 1 complex is tightly associated with a multiprotein complex TFIIH , which plays a dual role in transcription and DNA repair . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| Moreover , cells containing kin 28 ( T162A ) and a conditional allele of TFB 3 ( the ortholog of the mammalian MAT 1 protein , an assembly factor for MO 15 and cyclin H ) are severely compromised and display a significant further reduction in Kin28p activity . ^^^ This finding provides in vivo support for the previous biochemical observation that MO 15 cyclin H complexes can be activated either by activating phosphorylation of MO 15 or by binding to MAT 1 . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| These serines are phosphorylated in vitro by cdk 7 , a cyclin dependent kinase associated to cyclin H and MAT 1 in the CAK complex ( cdk7 . cyclin H . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| The CAK complex , which is also found free in the cell , is composed of cdk 7 , cyclin H , and MAT 1 . ^^^ The median portion of MAT 1 , which contains a coiled coil motif , allows the binding of CAK to the TFIIH core through interactions with both XPD and XPB helicases . ^^^ In the present work , we found that the C terminus of MAT 1 binds to the cdk 7 10 cyclin H complex and activates the cdk 7 kinase activity . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| CAK from humans contains p40MO15 ( cdk 7 ) , cyclin H and MAT 1 , which are also subunits of transcription factor IIH where they phosphorylate the C terminal domain of the large subunit of RNA polymerase 2 . ^^^ CAK from humans contains p40MO15 ( cdk 7 ) , cyclin H and MAT 1 , which are also subunits of transcription factor IIH where they phosphorylate the C terminal domain of the large subunit of RNA polymerase 2 . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| Cyclin dependent kinase 7 ( Cdk 7 ) forms a trimeric complex with cyclin H and Mat 1 to form the mammalian Cdk activating kinase , CAK , as well as a part of the basal transcription factor TFIIH , where Cdk 7 phosphorylates the C terminal domain ( CTD ) of the large subunit of RNA polymerase 2 . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| Immunolabeling experiments localize several subunits : p 44 , within the ring structure , forms the base of the protruding protein density which includes the cdk 7 kinase , cyclin H , and MAT 1 . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| The MAT 1 cyclin dependent kinase activating kinase ( CAK ) assembly / targeting factor interacts physically with the MCM 7 DNA licensing factor . ^^^ MAT 1 ( mnage trois 1 ) functions as an assembly / targeting factor of CAK ( cyclin dependent kinase activating kinase ) . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| MAT 1 modulated CAK activity regulates cell cycle G ( 1 ) exit . ^^^ We previously demonstrated that abrogation of MAT 1 ( mnage trois 1 ) , an assembly factor and targeting subunit of CAK , induces G ( 1 ) arrest ( L . ^^^ This result led us to investigate how deregulation of CAK by MAT 1 abrogation affects the cell cycle G ( 1 ) exit , a process that is regulated most closely by phosphorylation of retinoblastoma tumor suppressor protein ( pRb ) . ^^^ Using mammalian cellular models that undergo G ( 1 ) arrest evoked by antisense MAT 1 abrogation , we found that deregulation of CAK inhibits pRb phosphorylation and cyclin E expression , CAK phosphorylation of pRb is MAT 1 dose dependent but cyclin D1 / CDK4 independent , and MAT 1 interacts with pRb . ^^^ These results suggest that CAK is involved in the regulation of cell cycle G ( 1 ) exit while MAT 1 modulated CAK formation and CAK phosphorylation of pRb may determine the cell cycle specificity of CAK in G ( 1 ) progression . . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| When the ternary Cdk activating kinase ( CAK ) complex composed of Cdk 7 , cyclin H , and MAT 1 was used as bait , the xeroderma pigmentosum ( XP ) D helicase of transcription factor IIH ( TFIIH ) , among other proteins , was identified as an interacting partner . ^^^ Deletion mutant analyses demonstrated that the coiled coil and the hydrophobic domains of MAT 1 interlink the CAK complex directly with the N terminal domain of XPD . ^^^ In addition we show that upon interaction with MAT 1 , CAK inhibits the helicase activity of XPD . ^^^ It is not surprising that under these conditions some XPD mutations affect interactions not only with p 44 , but also with MAT 1 , thus preventing either the CAK inhibitory function within CAK . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| The first consists of three subunits , namely CDK 7 , cyclin H , and an assembly factor called MAT 1 , while the second consists of phospho CDK 7 and cyclin H . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| Together with TFIIH subunits cyclin H and Mat 1 , Cdk 7 kinase is also found in a trimer complex known as Cdk activating kinase ( CAK ) . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| The kinase subunits of TFIIH in animal cells , Cdk 7 , cyclin H , and MAT 1 , were independently isolated as an activity termed CAK ( Cdk activating kinase ) , which phosphorylates and activates cell cycle kinases . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| We previously showed that MAT 1 ( mnage trois 1 ) , an assembly factor and targeting subunit of the cyclin dependent kinase ( CDK ) activating kinase ( CAK ) , modulates CAK activities to regulate G ( 1 ) exit . ^^^ The switch to CAK hypophosphorylation of RARalpha is accompanied by decreased MAT 1 expression and MAT 1 fragmentation that occurs in the differentiating cells through the all trans retinoic acid ( ATRA ) mediated proteasome degradation pathway . ^^^ Because HL60R cells that harbor a truncated ligand dependent AF 2 domain of RARalpha do not demonstrate any changes in MAT 1 levels or CAK phosphorylation of RARalpha following ATRA stimuli , these biochemical changes appear to be mediated directly through RARalpha . ^^^ These studies indicate that significant changes in MAT 1 levels and CAK activities on RARalpha phosphorylation accompany the ATRA induced G ( 1 ) arrest and differentiation activation , which provide new insights to explore the inversely coordinated P / D transition at G ( 1 ) exit . . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| The mammalian Mat 1 protein has been implicated in cell cycle regulation as part of the Cdk activating kinase ( CAK ) , and in regulation of transcription as a subunit of transcription factor TFIIH . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| Here we report that three core components of the general transcription factor ( TF ) IIH , CDK 7 , Cyclin H , and MAT 1 , enhance Vpr ' s GR coactivator activity but inhibit its cell cycle arresting function . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| MAT 1 is an assembly / targeting factor for cyclin dependent kinase activating kinase ( CAK ) , which has been shown to functionally interact with general transcriptional factor TFIIH , a known inducer of ER transactivation . ^^^ Together , these findings identified MAT 1 as a target of MTA 1 and provided new evidence to suggest that the transactivation functions of ER might be influenced by the regulatory interactions between CAK and MTA 1 in breast cancer cells . . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| General transcription factor IIH ( TFIIH ) consists of nine subunits : cyclin dependent kinase 7 ( Cdk 7 ) , cyclin H and MAT 1 ( forming the Cdk activating kinase or CAK complex ) , the two helicases Xpb / Hay and Xpd , and p 34 , p 44 , p 52 and p 62 ( refs 1 3 ) . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| The enzyme , which contains Cdk 7 , cyclin H , and Mat 1 , specifically phosphorylates Ser 159 of Cdk 5 ( 149 163 ) and enhances Cdk5 / p25 activity . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| When influences of HCV core on CDK activating kinase ( CAK ) were examined , the expression levels of the CAK components , CDK 7 , cyclin H , and MAT 1 , were not affected . ^^^ The results showed that HCV core led to dissociation of MAT 1 from the CAK complex and suppressed the CAK activity . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| CAK activity in G 1 regulation is determined by its assembly factor and targeting subunit , mnage trois 1 ( MAT 1 ) . ^^^ Thus , these findings reveal an important mechanism by which MAT 1 modulated CAK activity is crucial in the switch from proliferation to differentiation in neuroblastoma cells . . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| For maximum activity and stability , CDK 7 requires phosphorylation , association with cyclin H , and association with a third protein , MAT 1 . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| Cyclin dependent kinase activating kinase ( CAK ) is a trimeric complex composed of cdk 7 , cyclin H and MAT 1 . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| We found several proteins to co purify with a Tat associated RNAPII CTD kinase activity including LIS 1 , CDK 7 , cyclin H , and MAT 1 . ^^^ Tat interacted with LIS 1 but not with CDK 7 , cyclin H or MAT 1 in vitro . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| The kinase is composed of three subunits : CDK 7 , Cyclin H and MAT 1 ( mnage a trois ) . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| Cdk activating kinase ( CAK ) is a trimeric complex consisting of cdk 7 , cyclin H , and MAT 1 , which activates the cell cycle regulating cdks through T loop phosphorylation . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| We previously found that ATRA induced cell differentiation accompanies ubiquitination proteolysis of mnage trois 1 ( MAT 1 ) , an assembly factor and targeting subunit of cyclin dependent kinase ( CDK ) activating kinase ( CAK ) that regulates G 1 exit . ^^^ In response to ATRA , PML / RARalpha is dissociated from CAK , leading to MAT 1 degradation , G 1 arrest , and decreased CAK phosphorylation of PML / RARalpha . ^^^ CAK phosphorylation of PML / RARalpha is inhibited when MAT 1 levels are reduced . ^^^ These findings reveal a novel ATRA signaling on APL cell differentiation , in which ATRA coordinates G 1 arrest and transition into differentiation by inducing MAT 1 degradation and PML / RARalpha hypophosphorylation through disrupting PML / RARalpha binding and phosphorylation by CAK . . ^^^ |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P51948 and P51946 |
Pubmed |
SVM Score :0.0 |
| NA |
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