| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| Recently , mutations in the beta subunit ( h beta ENaC ) and the gamma subunit ( h gamma ENaC ) of the human epithelial sodium channel ( hENaC ) have been shown to result in excessive elevation of blood pressure in patients with Liddle ' s syndrome . ^^^ RESULTS : Among 71 patients with hypertensive urgency ( 78 . 9 % ) and 19 patients with hypertensive emergency ( 21 . 1 % ) not one individual showed a mutation in genomic DNA extending from codon 532 to codon 637 of h beta ENaC and from codon 525 to codon 651 of h gamma ENaC . ^^^ CONCLUSIONS : The present study clearly demonstrates the absence of mutations in the carboxy terminus of the h beta ENaC and h gamma ENaC gene of hENaC in an Austrian cohort of 90 patients suffering from hypertensive crisis . . ^^^ |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| Acidification of the cytoplasmic solution also inhibited alpha , beta ENaC , alpha , gamma ENaC , and alpha ENaC currents . ^^^ |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| However , the half lives of alpha and gamma ENaC subunits that reached the apical cell surface were considerably longer ( t ( 12 ) > 24 h ) , whereas intriguingly , the half life of cell surface beta ENaC was only approximately 6 h . ^^^ Up regulation of ENaC mediated sodium conductance by overnight treatment with aldosterone or by short term incubation with vasopressin dramatically increased cell surface levels of beta ENaC without affecting alpha or gamma ENaC levels . ^^^ |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| We thus developed a protocol in which the alpha and gamma ENaC cDNA ' s were first fused individually with polyhedrin promoters at their 5 ' ends and then inserted in the multiple cloning sites of pVL 1393 transfer vector carrying the beta ENaC cDNA . ^^^ |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| The ENaC is a multimeric protein composed of alpha ENaC , beta ENaC , and gamma ENaC subunits . ^^^ |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| It consists of three homologous subunits , alpha ENaC , beta ENaC , and gamma ENaC ( for epithelial Na+ channel ) , which share significant identity with degenerins , a family of proteins found in the nematode Caenorhabditis elegans , and with ligand gated cation channels , such as FaNaC [ Phe Met Arg Phe NH 2 ( i . e . , FMRF amide ) Na+ channel ] or ASIC ( acid sensing ion channel ) , two neuronal ionotropic receptors for Phe Met Arg Phe NH 2 and H+ , respectively . ^^^ Gain of function mutations affecting beta ENaC and gamma ENaC genes can cause Liddle syndrome , a rare from of genetic hypertension . ^^^ Steroids strongly increase beta ENaC and gamma ENaC transcription in rat distal colon . ^^^ |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| Mutation of a cluster of Lys residues ( to Arg ) at the N terminus of gamma ENaC leads to both inhibition of ubiquitination and increased channel activity , an effect augmented by N terminal Lys to Arg mutations in alpha ENaC , but not in beta ENaC . ^^^ |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| In mice maintained on a low NaCl diet ( < 0 . 02 % NaCl ) , ( 1 ) the abundances of 2 aldosterone regulated transporters were markedly decreased in knockout versus wild type mice , namely , the thiazide sensitive cotransporter and the alpha subunit of the amiloride sensitive Na ( + ) channel ( alpha ENaC ) ; ( 2 ) the abundances of beta ENaC and gamma ENaC were markedly increased ; and ( 3 ) there were no significant changes in the abundances of the proximal tubule Na+ H ( + ) exchanger or the Na ( + ) K ( + ) 2Cl ( ) cotransporter of the thick ascending limb . ^^^ |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| HCTZ infusion increased the abundances of thiazide sensitive Na ( + ) Cl ( ) cotransporter and beta ENaC in the cortex and beta and gamma ENaC in the outer medulla . ^^^ |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| PMA inhibited the mRNA expression of all 3 ENaC subunits ( alpha ENaC : 56 . 0 % + / 12 . 1 % ; beta ENaC : 62 . 6 % + / 15 . 9 % ; gamma ENaC : 68 . 5 % + / 10 . 6 % , respectively ) and amiloride sensitive current ( control = 7 . 0 + / 1 . 5 microA / cm ( 2 ) ; PMA = 1 . 7 + / 0 . 9 microA / cm ( 2 ) ) significantly at 24 hours . ^^^ |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| Inhibition of Na+ entry by either method was associated with a profound decrease in apical membrane beta ENaC without significant changes in apical membrane alpha or gamma ENaC amounts . ^^^ Restoration of apical Na+ and / or removal of amiloride resulted in return of Isc to control levels over 2 h and coincided with return of apical beta ENaC to control levels without change in apical alpha or gamma ENaC . ^^^ |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| Immunoblotting revealed that lithium treatment induced a marked decrease in the protein abundance of beta ENaC and gamma ENaC in the cortex and outer medulla . ^^^ Moreover , immunohistochemistry and laser confocal microscopy demonstrated an almost complete absence of beta ENaC and gamma ENaC labeling in cortical and outer medullary collecting duct , which was not affected by dietary sodium intake . ^^^ These results identify a marked and highly segment specific downregulation of beta ENaC and gamma ENaC in the cortical and outer medullary collecting duct , chief sites for collecting duct sodium reabsorption , in rats with a lithium induced increase in fractional excretion of sodium . . ^^^ |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| A true two hybrid reaction employing full length IKKbeta and the carboxyl termini of all three subunits confirmed a strong interaction with beta ENaC , a weak interaction with gamma ENaC , and no interaction with alpha ENaC . ^^^ Immunoprecipitation with beta ENaC , but not gamma ENaC , resulted in co immunoprecipitation of IKKbeta . ^^^ |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| Under all conditions , the loss of function of SGK 1 caused a significant decrease in the expression of alpha and beta ENaC , but not gamma ENaC . ^^^ |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| RESULTS : The alpha ENaC , beta ENaC , and gamma ENaC proteins were expressed in human urinary bladder epithelium with outlet obstruction , and the alpha ENaC and gamma ENaC proteins were virtually unstained in the control bladders . ^^^ Alpha ENaC , beta ENaC , and gamma ENaC mRNA were detected in 1 , 6 , and 4 of 9 control bladders , respectively . ^^^ |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| In contrast , there was increased expression of three proteins normally expressed at higher levels in the cortical collecting duct ( CCD ) than in IMCD : AQP 3 ( another basolateral water channel ) and the epithelial sodium channel subunits beta ENaC and gamma ENaC . ^^^ Messenger RNA profiling ( real time RT PCR ) revealed changes in UT A 1 , beta ENaC , gamma ENaC , and AQP 3 transcript abundance that paralleled the changes in protein abundance . ^^^ As in the AQP 1 knockout mice , ClC nK 1 ( / ) mice showed decreased expression of UT A 1 and increased expression of beta ENaC and gamma ENaC vs . ^^^ |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| In real time quantitative PCR experiments , the addition of dexamethasone increased the content of claudin 4 , occludin , and Na+ channel gamma subunit ( gamma ENaC ) mRNAs by 1 . 34 , 1 . 32 , and 1 . 80 fold , respectively , after 1 h and was followed by an increase at 6 h in the content of mRNA of alpha and beta ENaC and of alpha 1 and beta 1 Na , K ATPase . ^^^ |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| Using reverse transcriptase polymerase chain reaction we observed a significant increase of alpha ENaC in the neuronal retina of DBA / 2J mice when compared with control animals , while beta ENaC and gamma ENaC were not detectable in this tissue . ^^^ Consistent with our polymerase chain reaction data , beta ENaC was not detected by specific antibodies in the retina , while gamma ENaC was only present in the retinal pigment epithelium under ocular hypertension . ^^^ |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| Immunoperoxidase microscopy confirmed the increased labeling of beta ENaC and gamma ENaC subunits in the late distal convoluted tubule , connecting tubule , and cortical and outer medullary collecting duct segments . ^^^ In contrast , subcellular localization of alpha ENaC , beta ENaC , and gamma ENaC was not changed . ^^^ |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| In many epithelial tissues , amiloride sensitive epithelial sodium channels ( ENaC ) are formed from three subunit proteins designated alpha ENaC , beta ENaC , and gamma ENaC . ^^^ |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| In most cortical areas , thalamus , amygdala , and suprachiasmatic nucleus , notable expression of gamma ENaC was undetectable , whereas alpha and beta ENaC were abundantly expressed pointing to the possibility of a heterogeneous population of channels . ^^^ |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| The protein abundances of beta ENaC and gamma ENaC were decreased in the cortex / OSOM while increased in the ISOM . ^^^ |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| Whole cell and confocal microscopy experiments were conducted in Madin Darby canine kidney cells co expressing alpha and beta ENaC only or with either gamma ENaC or gamma ( T ) ENaC . ^^^ |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| The mechanism of ENaC activation was similar in CCD from nephrotic and sodium depleted rats , as demonstrated by ( 1 ) increased number of active ENaC evaluated by patch clamp , ( 2 ) recruitment of ENaC to the apical membrane determined by immunohistochemistry , ( 3 ) shift in the electrophoretic profile of gamma ENaC , and ( 4 ) increased abundance of beta ENaC mRNA . ^^^ |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| Epithelial sodium channel genes Scnn1b and Scnn1g are closely linked on distal mouse chromosome 7 . ^^^ The chromosomal localizations of Scnn1b and Scnn1g , genes corresponding to the beta and gamma subunits , respectively , of an epithelial non voltage gated amiloride sensitive sodium channel , were determined by analyses of two sets of multilocus crosses using probes generated by polymerase chain reaction and a mouse kidney cortical collecting tubule cell line ( M 1 ) . ^^^ Scnn1b and Scnn1g were determined to be closely linked on distal mouse chromosome 7 , showing no recombination with Zp 2 , whereas the gene for the alpha subunit , Scnn1a , was confirmed to map to distal mouse chromosome 6 . . ^^^ |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| The amiloride sensitive epithelial sodium channel alpha , beta , and gamma subunit genes , Scnn1a , Scnn1b , and Scnn1g , and the thiazide sensitive sodium chloride cotransporter gene , Slc12a1 , have been mapped in the mouse using an interspecific backcross panel . ^^^ |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| These two chromosomal regions harbour the genes encoding the three subunits of the human amiloride sensitive epithelial sodium channel ( hENaC ) : SCNN1B and SCNN1G on 16p and SCNN1A on 12p . ^^^ |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| The two chromosomal regions harbour genes for subunits of the amiloride sensitive epithelial sodium channel : SCNN1B and SCNN1G on 16p and SCNN1A on 12p . ^^^ Liddle ' s syndrome of hypertension and pseudoaldosteronism has been shown to arise from mutations in SCNN1B and SCNN1G . ^^^ |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| Mutations in the Scnn1b and Scnn1g genes , which encode the beta and the gamma subunits of ENaC , cause a severe form of hypertension ( Liddle syndrome ) . ^^^ |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| Liddle ' s syndrome is an autosomal dominant disease characterized by sodium sensitive early hypertension and mutations in either the beta or gamma subunit of the amiloride sensitive epithelial sodium channel encoded by SCNN1B and SCNN1G . ^^^ We sequenced the 381 bp coding regions in exon 13 of SCNN1B and the 381 bp coding regions in exon 12 of SCNN1G in 948 and 953 Japanese patients with hypertension , respectively . ^^^ The roles of these missense mutations in the SCNN1B or SCNN1G gene identified in hypertensive patients are not clear in the pathogenesis of hypertension and the regulation of electrolytes . ^^^ |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| RT PCR analysis revealed the presence of both glucocorticoid and mineralocorticoid receptor mRNA as well as mRNA for the alpha and gamma subunits of the epithelia Na+ channels ( alpha and gamma ENaC ) , but not beta ENaC . ^^^ |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| Also unexpectedly , the amount of beta ENaC proteins was similar in both groups of mice but there was a reduction of one form of gamma ENaC in + / mice . ^^^ |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P51170 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|