| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| In pulse chase studies of individually expressed subunits , we found that the Triton soluble form of beta ENaC accumulated initially , whereas the Triton soluble form of alpha ENaC decreased throughout the time course . ^^^ |
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| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| The ENaC is a multimeric protein composed of alpha ENaC , beta ENaC , and gamma ENaC subunits . ^^^ |
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| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| In mice maintained on a low NaCl diet ( < 0 . 02 % NaCl ) , ( 1 ) the abundances of 2 aldosterone regulated transporters were markedly decreased in knockout versus wild type mice , namely , the thiazide sensitive cotransporter and the alpha subunit of the amiloride sensitive Na ( + ) channel ( alpha ENaC ) ; ( 2 ) the abundances of beta ENaC and gamma ENaC were markedly increased ; and ( 3 ) there were no significant changes in the abundances of the proximal tubule Na+ H ( + ) exchanger or the Na ( + ) K ( + ) 2Cl ( ) cotransporter of the thick ascending limb . ^^^ |
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| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| Immunohistochemical localization with antibodies against alpha ENaC and beta ENaC showed labeling in Mller cells and neurons , respectively . ^^^ The presence of alpha ENaC , beta ENaC , and ASIC 1 was detected by Western blotting . ^^^ |
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| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| Our analyses revealed the following homozygous mutations in three probands : 1 ) insertion of a T in exon 8 of the alpha ENaC gene that causes a frameshift error at Tyr ( 447 ) and leads to a premature stop codon at K 459 in a Pakistani patient ; 2 ) R508stop mutation in exon 11 of the alpha ENaC gene in an Indian patient ; and 3 ) a splice site mutation in intron 12 of the beta ENaC gene ( 1669 + 1 g > a ) in a Scottish patient . ^^^ |
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| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| PMA inhibited the mRNA expression of all 3 ENaC subunits ( alpha ENaC : 56 . 0 % + / 12 . 1 % ; beta ENaC : 62 . 6 % + / 15 . 9 % ; gamma ENaC : 68 . 5 % + / 10 . 6 % , respectively ) and amiloride sensitive current ( control = 7 . 0 + / 1 . 5 microA / cm ( 2 ) ; PMA = 1 . 7 + / 0 . 9 microA / cm ( 2 ) ) significantly at 24 hours . ^^^ |
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| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| It consists of three homologous subunits , alpha ENaC , beta ENaC , and gamma ENaC ( for epithelial Na+ channel ) , which share significant identity with degenerins , a family of proteins found in the nematode Caenorhabditis elegans , and with ligand gated cation channels , such as FaNaC [ Phe Met Arg Phe NH 2 ( i . e . , FMRF amide ) Na+ channel ] or ASIC ( acid sensing ion channel ) , two neuronal ionotropic receptors for Phe Met Arg Phe NH 2 and H+ , respectively . ^^^ Loss of function mutations affecting alpha ENaC or beta ENaC genes can cause pseudohypoaldosteronism type 1 . ^^^ |
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| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| Mutation of a cluster of Lys residues ( to Arg ) at the N terminus of gamma ENaC leads to both inhibition of ubiquitination and increased channel activity , an effect augmented by N terminal Lys to Arg mutations in alpha ENaC , but not in beta ENaC . ^^^ |
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| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| Acidification of the cytoplasmic solution also inhibited alpha , beta ENaC , alpha , gamma ENaC , and alpha ENaC currents . ^^^ |
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| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| In many epithelial tissues , amiloride sensitive epithelial sodium channels ( ENaC ) are formed from three subunit proteins designated alpha ENaC , beta ENaC , and gamma ENaC . ^^^ |
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| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| In group A , protein abundance of alpha ENaC was unchanged , whereas beta ENaC abundance was decreased in the cortex / outer stripe of outer medulla compared with controls . ^^^ |
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| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| CDH lungs showed a 67 % reduction in alpha ENaC and beta ENaC expression , but no change in alpha 1 Na K ATPase expression . ^^^ |
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| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| Western blot analysis showed up regulation of expression of the alpha subunit of the epithelial sodium channel ( alpha ENaC ) , the beta subunit of the epithelial sodium channel ( beta ENaC ) , sodium / hydrogen exchanger ( NHE ) 3 , and the renal outer medulla K ( + ) channel ( ROMK ) in HF rats , whereas expression of the gamma subunit of the epithelial sodium channel and the alpha 1 subunit of Na ( + ) K ( + ) ATPase remained unchanged . ^^^ Thus , HF treatment caused the reduction of renal CYP4A1 and CYP4A8 expression , whereas the increases in alpha ENaC , beta ENaC , NHE 3 , and ROMK expression in renal tubules may have contributed sodium retention and hypertension in HF rats . ^^^ |
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| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| Using reverse transcription polymerase chain reaction in rat taste tissues , we have identified two alternatively spliced transcripts of ENAC ( alpha ENACa and alpha ENACb ) with deletions of nucleotides that introduce a premature stop codon and may result in proteins shortened by 199 and 216 amino acids , respectively , at the carboxyl terminus . ^^^ |
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| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| The frequency of observance of this channel was significantly reduced by antisense oligonucleotide against alpha ENaC but not by antisense oligonucleotide against beta ENaC , indicating that only the alpha subunit of ENaC is necessary to form stretch activated cation channels . ^^^ |
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| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| A true two hybrid reaction employing full length IKKbeta and the carboxyl termini of all three subunits confirmed a strong interaction with beta ENaC , a weak interaction with gamma ENaC , and no interaction with alpha ENaC . ^^^ |
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| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| RESULTS : The alpha ENaC , beta ENaC , and gamma ENaC proteins were expressed in human urinary bladder epithelium with outlet obstruction , and the alpha ENaC and gamma ENaC proteins were virtually unstained in the control bladders . ^^^ Alpha ENaC , beta ENaC , and gamma ENaC mRNA were detected in 1 , 6 , and 4 of 9 control bladders , respectively . ^^^ |
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| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| Using reverse transcriptase polymerase chain reaction we observed a significant increase of alpha ENaC in the neuronal retina of DBA / 2J mice when compared with control animals , while beta ENaC and gamma ENaC were not detectable in this tissue . ^^^ |
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| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| In contrast , subcellular localization of alpha ENaC , beta ENaC , and gamma ENaC was not changed . ^^^ |
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| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| In mice , this channel is composed of three subunits ( alpha , beta , and gamma ) , which are encoded by different genes ( Scnn1a , Scnn1b , and Scnn1c , respectively ) . ^^^ |
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| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| Exons 13 of Scnn1a and Scnn1b , which encode the second transmembrane segment and the COOH termini of alpha and beta ENaC , respectively , were amplified from pooled DNA samples of members of each family by PCR . ^^^ Use of constant denaturant capillary electrophoresis of pooled blood samples to identify single nucleotide polymorphisms in the genes ( Scnn1a and Scnn1b ) encoding the alpha and beta subunits of the epithelial sodium channel . ^^^ |
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| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| ENaCalpha mRNA expression was also observed in medullary and cortical thick ascending limbs ( MAL and CAL , respectively ) , while ENaCbeta and gamma mRNA expressions were not observed . ^^^ The absence of stimulation of ENaCalpha mRNA expression in collecting ducts suggests the physiological significance of ENaCbeta and gamma mRNA for acute regulation by vasopressin . ^^^ |
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| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| Scnn1b and Scnn1g were determined to be closely linked on distal mouse chromosome 7 , showing no recombination with Zp 2 , whereas the gene for the alpha subunit , Scnn1a , was confirmed to map to distal mouse chromosome 6 . . ^^^ |
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| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| The amiloride sensitive epithelial sodium channel alpha , beta , and gamma subunit genes , Scnn1a , Scnn1b , and Scnn1g , and the thiazide sensitive sodium chloride cotransporter gene , Slc12a1 , have been mapped in the mouse using an interspecific backcross panel . ^^^ |
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| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| These two chromosomal regions harbour the genes encoding the three subunits of the human amiloride sensitive epithelial sodium channel ( hENaC ) : SCNN1B and SCNN1G on 16p and SCNN1A on 12p . ^^^ |
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| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| The two chromosomal regions harbour genes for subunits of the amiloride sensitive epithelial sodium channel : SCNN1B and SCNN1G on 16p and SCNN1A on 12p . ^^^ |
|
| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37088 and P51168 |
Pubmed |
SVM Score :0.0 |
| NA |
|