| Interacting proteins: Q9UNE2 and P51159 |
Pubmed |
SVM Score :0.65531943 |
| In the present study we showed by means of deletion and mutation analyses that rabphilin and Noc 2 are recruited to dense core vesicles through specific interaction with Rab27A , not with Rab3A , in PC 12 cells . 0.65531943^^^ |
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| Interacting proteins: Q9UNE2 and P51159 |
Pubmed |
SVM Score :0.0 |
| In the beta cell line INS 1E wild type Noc 2 , Noc265E , and Noc258A , a mutant capable of interacting with Rab 27 but not Rab 3 , colocalized with insulin containing vesicles . ^^^ |
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| Interacting proteins: Q9UNE2 and P51159 |
Pubmed |
SVM Score :0.0 |
| Assay of the Rab binding specificity of rabphilin and Noc 2 : target molecules for Rab 27 . ^^^ In this chapter we describe various assay methods that have been used to characterize the RBD of rabphilin and Noc 2 as `` RBD 27 ( Rab binding domain for Rab 27 ) . ' ' . ^^^ However , the RBD of rabphilin and Noc 2 has been shown to bind Rab27A ( the closest homologue of Rab 3 isoforms ) in preference to Rab3A , both in vitro and in vivo . ^^^ Rabphilin and Noc 2 are recruited to dense core vesicles ( DCVs ) in neuroendocrine PC 12 cells and regulate their exocytosis through interaction with Rab27A rather than with Rab3A . ^^^ Rab3A binding defective mutants of rabphilin ( E50A ) and Noc 2 ( E51A ) retain the ability to target DCVs in PC 12 cells , the same as the wild type proteins , whereas Rab27A binding defective mutants of rabphilin ( E50A / I54A ) and Noc 2 ( E51A / I55A ) do not ( i . e . , they are present throughout the cytoplasm ) . ^^^ |
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| Interacting proteins: Q9UNE2 and P51159 |
Pubmed |
SVM Score :0.0 |
| The GTPases Rab3a and Rab27a and their effectors Granuphilin / Slp4 and Noc 2 are essential regulators of neuroendocrine secretion . ^^^ ICER overexpression diminished the level of Granuphilin , Noc 2 , Rab3a and Rab27a by binding to cAMP responsive elements located in the promoters of these genes and inhibited exocytosis of beta cells in response to secretagogues . ^^^ |
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