| Interacting proteins: Q8TDW5 and P51159 |
Pubmed |
SVM Score :0.70287667 |
| Expression of Slp 5 protein and in vivo association of Slp 5 with Rab27A in the mouse liver were further confirmed by immunoprecipitation . 0.70287667^^^ |
|
| Interacting proteins: Q8TDW5 and P51159 |
Pubmed |
SVM Score :0.0 |
| Rab27a regulates epithelial sodium channel ( ENaC ) activity through synaptotagmin like protein ( SLP 5 ) and Munc 13 4 effector mechanism . ^^^ In order to further explore the molecular mechanism of this regulation , we performed competitive assays with two Rab27a binding proteins : synaptotagmin like protein ( SLP 5 ) and Munc 13 4 ( a putative priming factor for exocytosis ) . ^^^ The SLP 5 reversal of Rab27a effect was restricted to C terminal C2A / C2B domains assigned for putative phospholipids binding function while the Rab27a binding SHD motif imparted higher inhibition . ^^^ The ENaC mediated currents remain unaffected by Rab27a though SLP 5 appears to strongly bind it . ^^^ The immunoprecipitation experiments suggest that in the presence of excessive Munc 13 4 and SLP 5 proteins , Rab27a interaction with ENaC is diminished . ^^^ |
|
| Interacting proteins: Q8TDW5 and P51159 |
Pubmed |
SVM Score :0.0 |
| Rab27a mediated effects could be reversed by Rab27a binding proteins , the synaptotagmin like protein ( SLP 5 ) and Munc 13 4 accessory protein ( a putative priming factor for exocytosis ) . ^^^ Munc 13 4 and SLP 5 , on the other hand , limit Rab27a availability to CFTR , thus minimizing its effect on channel function . ^^^ These observations decisively prove that Rab27a is involved in CFTR channel regulation through protein protein interactions involving Munc 13 4 and SLP 5 effector proteins , and thus could be a potential target for cystic fibrosis therapy . . ^^^ |
|
| Interacting proteins: Q8TDW5 and P51159 |
Pubmed |
SVM Score :0.0 |
| NA |
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