| Interacting proteins: Q8NFW9 and P51159 |
Pubmed |
SVM Score :0.5845782 |
| As with other Slac 2 members and the Slp family , the Slp homology domain of Slac 2 c was found to interact specifically with the GTP bound form of Rab27A / B both in vitro and in intact cells , and the C terminal domain of Slac 2 c interacted with myosin Va and myosin VIIa . 0.5845782^^^ |
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| Interacting proteins: Q8NFW9 and P51159 |
Pubmed |
SVM Score :1.3896431 |
| In vitro Slac 2 c simultaneously directly interacts with both Rab27A and an actin based motor protein , myosin Va , via its N terminal SHD and middle region , respectively , consistent with the fact that the overall structure of Slac 2 c is similar to that of Slac 2 a / melanophilin , a linker protein between Rab27A and myosin Va in the melanosome transport in melanocytes . 1.3896431^^^ In this chapter we describe the methods that have been used to analyze the protein protein interactions of Slac 2 c , specifically with Rab27A , myosin Va / VIIa , and actin . . 0.67729477^^^ |
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| Interacting proteins: Q8NFW9 and P51159 |
Pubmed |
SVM Score :0.0 |
| RT PCR analysis demonstrates that transcripts for Rab27a and Rab27b and Slac 2 c ( a protein that links Rab27a / b to myosin Va / VIIa ) are expressed in testis . ^^^ |
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| Interacting proteins: Q8NFW9 and P51159 |
Pubmed |
SVM Score :0.0 |
| Slac 2 c / MyRIP , an in vitro Rab27A and myosin Va / VIIa binding protein , has recently been proposed to regulate retinal melanosome transport in retinal pigment epithelium cells by directly linking melanosome bound Rab27A and myosin VIIa ; however , the exact function of Slac 2 c in melanosome transport has never been clarified . ^^^ Consistent with this result , cyan fluorescent protein tagged Slac 2 c expressed in melanocytes was localized on melanosomes via the specific interaction with Rab27A and recruited co expressed yellow fluorescent protein tagged myosin VIIa to the melanosomes without interfering with the normal peripheral melanosome distribution , whereas when myosin VIIa alone was expressed in melanocytes , it was not localized on the melanosomes . ^^^ Based on these findings , we propose that Slac 2 c acts as a functional myosin VIIa receptor and that the Rab27A . ^^^ |
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| Interacting proteins: Q8NFW9 and P51159 |
Pubmed |
SVM Score :0.0 |
| Rab27A and its effector MyRIP link secretory granules to F actin and control their motion towards release sites . ^^^ The GTPase Rab27A interacts with myosin VIIa and myosin Va via MyRIP or melanophilin and mediates melanosome binding to actin . ^^^ Here we show that Rab27A and MyRIP are associated with secretory granules ( SGs ) in adrenal chromaffin cells and PC 12 cells . ^^^ Overexpression of Rab27A , GTPase deficient Rab27A Q78L , or MyRIP reduced secretory responses of PC 12 cells . ^^^ Amperometric recordings of single adrenal chromaffin cells revealed that Rab27A Q78L and MyRIP reduced the sustained component of release . ^^^ |
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| Interacting proteins: Q8NFW9 and P51159 |
Pubmed |
SVM Score :0.0 |
| In the retinal pigment epithelium , a similar complex formed by Rab27a , a melanophilin homolog called MyRIP and myosin VIIa is probably responsible for the tethering of melanosomes to the actin cytoskeleton . ^^^ |
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