| Interacting proteins: P50402 and P13569 |
Pubmed |
SVM Score :0.0 |
| Microsomes from ISO and betaNF dosed males showed a reduction ( P < 0 . 05 ) in EMD activity when compared with control microsomes , and CF was without effect . ^^^ In females , EMD activities were significantly increased in microsomes from PCN , DEX and PB dosed but not betaNF , ISO , or CF dosed animals . ^^^ |
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| Interacting proteins: P50402 and P13569 |
Pubmed |
SVM Score :0.0 |
| The 17 2 CF activity was not neutralized after pretreatment with polyclonal anti STa antibody . ^^^ |
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| Interacting proteins: P50402 and P13569 |
Pubmed |
SVM Score :0.0 |
| The involvement of CFTR suggests a possible cystic fibrosis heterozygote advantage against STa induced diarrhea . . ^^^ |
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| Interacting proteins: P50402 and P13569 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND & AIMS : Guanylin and heat stable enterotoxin ( STa ) stimulate intestinal Cl secretion via activation of the cystic fibrosis transmembrane regulator ( CFTR ) encoded Cl channel . ^^^ CONCLUSIONS : Guanylin and STa stimulate electrogenic HCO 3 secretion in rat duodenum , most likely via CFTR Cl channel activation , but the different relationship for HCO 3 to Isc in cGMP than in cAMP stimulated anion secretion suggests a different cellular source and / or signaling pathways . . ^^^ |
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| Interacting proteins: P50402 and P13569 |
Pubmed |
SVM Score :0.0 |
| Remaining samples from strain RB 51 vaccinates tested negative on the STA , complement fixation ( CF ) , rivanol , and particle concentration fluorescence immunoassay ( PCFIA ) confirmatory tests . ^^^ |
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| Interacting proteins: P50402 and P13569 |
Pubmed |
SVM Score :0.0 |
| Whereas the type 1 kinase mediates numerous effects of natriuretic peptides and nitric oxide in cardiovascular cells , the type 2 kinase transduces signals from the Escherichia coli heat stable enterotoxin , STa , and from the endogenous intestinal peptide , guanylin , stimulating Cl conductance of the cystic fibrosis transmembrane conductance regulator ( CFTR ) . ^^^ |
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| Interacting proteins: P50402 and P13569 |
Pubmed |
SVM Score :0.0 |
| Binding of STa , or of the mammalian endogenous ligands guanylin and uroguanylin , activates the guanylyl cyclase C receptor ( GC C ) ; the resulting elevation of cGMP levels stimulates chloride secretion via CFTR . ^^^ |
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| Interacting proteins: P50402 and P13569 |
Pubmed |
SVM Score :0.0 |
| Forskolin , PGE 2 , 8 bromo cAMP and VIP ( cAMP dependent agonists ) , heat stable enterotoxin of Escherichia coli ( STa ) , guanylin and 8 bromo cGMP ( cGMP dependent agonists ) and carbachol ( Ca2+ dependent ) stimulated both the short circuit current ( Isc ) and the HCO 3 secretory rate ( JHCO 3 ) in all intestinal segments in normal mice , whereas none of these agonists had any effect on JHCO 3 in the intestine of CF mice . 3 . ^^^ |
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| Interacting proteins: P50402 and P13569 |
Pubmed |
SVM Score :0.0 |
| These cells possess transcripts for cystic fibrosis transmembrane conductance regulator , Na+ K+ 2Cl cotransporter , STa receptor , and intestine specific cGMP dependent protein kinase 2 . ^^^ |
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| Interacting proteins: P50402 and P13569 |
Pubmed |
SVM Score :0.0 |
| This work describes the first epidemiological survey of Burkholderia cepacia involved in pulmonary infections among the Portuguese population with cystic fibrosis ( CF ) who attended the major CF treatment Center in Lisbon at Sta . ^^^ |
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| Interacting proteins: P50402 and P13569 |
Pubmed |
SVM Score :0.0 |
| Uroguanylin , a natriuretic peptide hormone , is an endogenous ligand for the same guanylate cyclase C that the Escherichia coli heat stable enterotoxin ( STa ) binds when it causes secretory diarrhea by activating the cystic fibrosis transmembrane conductance regulator , thus altering fluid balance in the intestine . ^^^ |
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| Interacting proteins: P50402 and P13569 |
Pubmed |
SVM Score :0.0 |
| However , in CF , carbachol resulted in an electroneutral HCO ( 3 ) ( ) secretion , whereas STa induced electrogenic HCO ( 3 ) ( ) secretion that was similar to NL . ^^^ Although duodenal bicarbonate secretion is impaired in CF tissues , alternate pathway ( s ) likely exist that can be activated by carbachol and STa . . ^^^ |
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| Interacting proteins: P50402 and P13569 |
Pubmed |
SVM Score :0.0 |
| The structure and concentration dependent inhibition shown indicates that CFTR mediates both STa and LT stimulated colonic secretion . ^^^ |
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| Interacting proteins: P50402 and P13569 |
Pubmed |
SVM Score :0.0 |
| A single intraperitoneal injection of CFTR ( inh ) 172 ( 200 microg ) blocked intestinal fluid secretion in a rat closed loop model by > 90 % for cholera toxin and > 70 % for STa Escherichia coli toxin . ^^^ In mice , CFTR ( inh ) 172 ( 20 microg ) inhibited cholera toxin induced intestinal fluid secretion by 90 % ( persistence t ( 1 / 2 ) approximately 10 hours , K ( 1 ) approximately 5 microg ) and STa toxin by 75 % ( K ( 1 ) approximately 10 microg ) . ^^^ |
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| Interacting proteins: P50402 and P13569 |
Pubmed |
SVM Score :0.0 |
| Duodenal biopsies from CF patients secrete bicarbonate in response to heat stable enterotoxin from Escherichia coli ( STa ) but not cAMP . ^^^ To explore the mechanism of STa induced bicarbonate secretion in CF more fully , we examined the role of CFTR in STa stimulated duodenal bicarbonate secretion in mice . ^^^ However , both control and CF animals responded to STa with significant increases in bicarbonate output . ^^^ In vitro , STa stimulated bicarbonate secretion in CF tissues was inhibited by genistein , DIDS , and chloride free conditions , whereas bicarbonate secretion persisted in control mice . ^^^ In the CF duodenum , STa can stimulate bicarbonate secretion via tyrosine kinase activity resulting in apical Cl ( ) / HCO ( 3 ) ( ) exchange . ^^^ |
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| Interacting proteins: P50402 and P13569 |
Pubmed |
SVM Score :0.0 |
| STa and cGMP stimulate CFTR translocation to the surface of villus enterocytes in rat jejunum and is regulated by protein kinase G . ^^^ Enterotoxins released by Vibrio cholerae ( cholera toxin ) and Escherichia coli ( heat stable enterotoxin , or STa ) activate intracellular cAMP and cGMP and signal CFTR on the apical plasma membrane of small intestinal enterocytes to elicit chloride and fluid secretion . cAMP activates PKA , whereas cGMP signals a cGMP dependent protein kinase ( cGKII ) to phosphorylate CFTR in the intestine . ^^^ We used STa , cell permeant cGMP , and cAMP agonists in conjunction with PKG and PKA inhibitors , respectively , in rat jejunum to examine whether 1 ) cGMP and cGK 2 regulate the translocation of CFTR to the apical membrane and its relevance to fluid secretion , and 2 ) PKA regulates cAMP dependent translocation of CFTR because this intestinal segment is a primary target for toxigenic diarrhea . ^^^ STa and cGMP induced a greater than fourfold increase in surface CFTR in enterocytes in association with fluid secretion that was inhibited by PKG inhibitors . cAMP agonists induced a translocation of CFTR to the cell surface of enterocytes that was prevented by PKA inhibitors . ^^^ |
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| Interacting proteins: P50402 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P50402 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P50402 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P50402 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P50402 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P50402 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P50402 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P50402 and P13569 |
Pubmed |
SVM Score :0.0 |
| NA |
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