| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| The genes encoding lysozyme , lactoferrin , cathelicidin antimicrobial peptide ( hCAP18 / LL 37 ) , cathepsin G , bactericidal / permeability increasing protein , azurocidin ( CAP37 / heparin binding protein ) , and neutrophil elastase were each found to be single copy per haploid genome . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| These alterations of intraoocyte cAMP contents following exposure to forskolin paralleled those observed in human CG treated ovaries . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| EGF resulted in an increase in basal and cAMP induced human CG secretions in the trophoblasts in a similar manner as in the inhibin secretion . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| The suppression of clusterin gene expression can also be demonstrated by treatment with human CG or forskolin , which were known to elevate intracellular cAMP levels . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| Cholera toxin ( CT ) elevates the cellular levels of the second messenger cAMP and increases the secretion of CG and steroids in these cells , thus being a potent inducer of trophoblast differentiated functions . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| Our studies also show that the LH / CG induced down regulation of the LH / CG receptor mRNA is mediated by cAMP . ^^^ Thus , addition of 8 bromo cAMP to MA 10 cells leads to a similar reduction in the levels of LH / CG receptor and receptor mRNA ; while deglycosylated human CG , a hormone derivative that binds to the LH / CG receptor but has a reduced ability to stimulate cAMP synthesis , does not reduce the levels of LH / CG receptor mRNA . ^^^ Last , human CG or 8 bromo cAMP are unable to reduce LH / Cg receptor mRNA in a mutant MA 10 cell line that express a cAMP resistant phenotype . . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| The CG induced change in follicle membrane potential has previously been shown to be caused by potassium efflux produced by rises in intracellular cAMP . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| Although it is clear that cAMP is an important mediator of the actions of LH / CG in Leydig cells , recent studies from several laboratories have shown that the functions of Leydig cells can also be modulated by hormones and growth factors that do not appear to use cAMP as a second messenger . ^^^ Further characterization of one of the transfectants ( designated MA 10 ( K 3 ) ) revealed a parallel reduction in the ability of cAMP and human CG to induce cell rounding , to increase steroid synthesis , or to induce c fos mRNA . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| Deglycosylated hCG is a derivative of hCG which retains the ability to bind to the LH / CG receptor , but is unable to activate adenylyl cyclase and thus stimulate cAMP production . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| The full length CG beta promoter , 3700 to 362 basepairs ( bp ) , was stimulated 8 to 14 fold by treatment with 1 mM 8 bromo cAMP . ^^^ Analyses of a series of deletion mutants in the CG beta promoter demonstrated that 311 CG beta LUC retained nearly complete cAMP stimulation , but deletion to 187 bp eliminated cAMP responsiveness . ^^^ Recombinant CREB ( expressed in E . coli ) did not bind to these sites , and they share no sequence homology with the alpha gene CRE , indicating that a cAMP responsive transcription factor other than CREB interacts with the CG beta promoter . . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| After medium changes , human CG ( hCG ) , 8 bromo cAMP , or forskolin was added with or without IL 2 . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| We also used vectors designed to determine whether regions containing a putative CG beta CRE could confer cAMP responsiveness to a heterologous promoter . ^^^ Surprisingly , division of the hCG beta 5 ' flanking and untranslated regions into smaller fragments led to loss of cAMP responsiveness , suggesting that the hCG beta CRE may be comprised of multiple domains dispersed across the proximal 650 base pairs of CG beta 5 ' flanking and untranslated sequence . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| In an effort to characterize further the involvement of cAMP in the activation of Leydig cell steroidogenesis by lutropin / choriogonadotropin ( LH / CG ) , we examined the effects of these cyclic nucleotide analogues on a clonal strain of cultured murine Leydig tumor cells ( designated MA 10 ) . ^^^ Our results show that ( 1 ) ( Sp ) cAMPS activates and ( Rp ) cAMPS inhibits the isolated cAMP dependent protein kinase of the MA 10 cells ; ( 2 ) both analogues inhibit the isolated cAMP phosphodiesterase ( s ) ; ( 3 ) ( Sp ) cAMPS activates steroid biosynthesis in intact cells , but ( Rp ) cAMPS does not ; and ( 4 ) ( Rp ) cAMPS is a competitive inhibitor of the activation of steroidogenesis by ( Sp ) cAMPS , 8 bromo cAMP , human CG , cholera toxin , and forskolin . ^^^ However , ( Rp ) cAMPS is a more effective inhibitor when steroidogenesis is activated by ( Sp ) cAMPS or 8 bromo cAMP than when it is activated by human CG , cholera toxin , or forskolin . ^^^ We conclude that cAMP is a quantitatively important mediator of the activation of steroidogenesis by LH / CG even at low concentrations of hormone where an increase in steroid biosynthesis can not be easily correlated with increased cAMP accumulation . ^^^ Thus , our data indicate that if other second messengers are involved in the activation of steroidogenesis by LH / CG , they must do so by acting together with , rather than independently of , cAMP . . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| The alpha and beta subunit genes encoding chorionic gonadotropin ( CG ) are regulated transcriptionally in placental cells by cyclic AMP ( cAMP ) . ^^^ CG beta CAT was expressed preferentially in JEG 3 choriocarcinoma cells , and expression was markedly stimulated by treatment with 8 bromo cAMP . ^^^ The kinetics for cAMP stimulation of alpha CAT and CG beta CAT expression were different , suggesting that different pathways may be involved in cAMP stimulated expression of the alpha and CG beta genes . . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| The alpha subunit of human chorionic gonadotropin ( CG ) contains a discrete cAMP response element in the 5 ' flanking region of the gene . ^^^ Since cAMP also stimulates the synthesis of the CG beta subunit the presence of a cAMP cis element in the CG beta gene was examined . ^^^ These data suggest that the response of the CG alpha and beta genes to the non cyclic adenine derivatives occurs by a mechanism that differs from cAMP . . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| When inserted as an oligonucleotide with restriction enzyme linker sites , the 8 bp CRE itself is as active in conferring cAMP responsivity on an enhancerless chloramphenicol acetyltransferase reporter plasmid as is a single copy of the choriogonadotropin alpha ( CG alpha ) , twice repeated 18 bp sequence containing the CRE . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| In cultured 20 week human testis cells , cAMP increased MIF mRNA 8 . 3 fold , but the human gonadotropins FSH and CG had no effect . ^^^ In cultured adult human granulosa cells , CG and cAMP increased MIF mRNA accumulation to 430 % and 890 % , respectively , but FSH had no effect . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| Dexamethasone also enhanced the stimulatory effects of 8 bromo cAMP on CG secretion . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| We find that a major component of cAMP stimulation of alpha and CG beta mRNAs is independent of new protein synthesis . ^^^ However , cycloheximide treatment did not affect the degree of cAMP stimulation of alpha and CG beta mRNA levels which increased 20 and 26 fold , respectively . ^^^ Similarly , cycloheximide did not block cAMP stimulated transcription of the alpha and CG beta genes . ^^^ The effect of cAMP treatment on alpha and CG beta mRNA stability was assessed by decay after removal of cAMP , pulse chase analyses , and decay after inhibition of RNA synthesis by actinomycin D . ^^^ The half lives of alpha and CG beta mRNAs determined by decay rates after removal of cAMP were 6 . 0 h and 7 . 2 h , respectively . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| These results raise the possibility that the marked increase in PGS enzyme in granulosa cells of PO follicles that occurs as a consequence of the LH / human CG surge may not involve a cAMP regulated increase in transcription of the PGS gene itself . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| The binding of these derivatives to the LH / CG receptor did not differ significantly from purified CG ( CR 119 ) , but the ability of the mutant hormones to stimulate cAMP biosynthesis in vitro is reduced compared to wild type CG or CR 119 . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| Treatment of human granulosa cells with human CG ( hCG ) or an analog of its second messenger , cAMP , promotes a rapid increase in low density lipoprotein ( LDL ) receptor mRNA content . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| PMA added together with suboptimal doses of human CG , 8Br cAMP , cholera toxin , or forskolin significantly increased the amount of progesterone produced . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| The concentration of cyclic adenosine monophosphate ( cAMP ) and cyclic guanosine monophosphate ( cGMP ) was measured in developing chick ciliary ( CG ) and dorsal root ganglia ( DRG ) as a function of embryonic age from day 8 through day 18 using radioimmunoassays . ^^^ The concentration of cAMP and cGMP increased in both ganglia from day 8 through day 14 . cAMP levels were nearly two fold higher in DRG than in CG . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| The effect of bovine corneal extracellular matrix ( ECM ) on gonadotropin primed rat granulosa cells in vitro was studied by examining the following parameters : 1 ) rate of cell attachment to culture dishes ; 2 ) modulation of cell morphology ; 3 ) specific binding of [ 125I ] human ( h ) CG to LH / hCG receptors ; 4 ) cAMP response to hCG stimulation ; and 5 ) basal and hCG stimulated progesterone production . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| In placental explants and cell lines , cAMP stimulates the biosynthesis of CG alpha and beta subunits and produces marked stimulation of CG secretion . ^^^ We investigated the effect of a cell permeable analog , 8 bromo cAMP ( 8 Br cAMP ) , on CG alpha and beta subunit gene expression in the JEG 3 placental cell line . ^^^ RNA blot hybridization analysis demonstrated that 8 Br cAMP increased CG alpha and beta mRNA levels 15 to 30 fold , consistent with 8 Br cAMP regulation of CG biosynthesis at a pretranslational level . ^^^ Transcription rates for CG alpha and beta subunit genes were determined in the absence and presence of 8 Br cAMP using nuclear run on assays . ^^^ Expression of chimeric genes , consisting of alpha gene [ 1 . 5 kilobase ( Kb ) ] or CG beta gene ( 0 . 3 Kb ) 5 ' flanking sequences ligated to the coding sequence of a reporter enzyme chloramphenicol acetyl transferase ( CAT ) , was used to analyze the mechanism of 8 Br cAMP stimulation of gene transcription . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| The data indicated that the cG kinase , like its cAMP dependent protein kinase homologue , possesses two highly conserved intrachain cyclic nucleotide binding sites which have different dissociation rates and analog specificity . ^^^ E 2 + 4 cGMP in equilibrium E 2 . cGMP 4 The cG kinase could be photoaffinity labeled using 8 azido [ 32P ] cAMP . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| We investigated a possible role of cyclic adenosine 3 ' , 5 ' monophosphate ( cAMP ) in the neurotrophic activity of skeletal muscle conditioned medium ( SCM ) on CG explants . ^^^ We showed that treatment with cAMP or dibutyryl cAMP stimulated neuritogenesis of CG explants in a dose dependent fashion . ^^^ These observations suggested that the two neuritogenic agents do not act through identical mechanisms , a conclusion supported by experiments demonstrating that stimulation of the CG with dibutyryl cAMP enhanced ornithine decarboxylase ( ODC ) activity relative to controls , whereas stimulation with SCM had no effect on ODC activity . ^^^ We conclude that although cAMP does exhibit neuritogenic activity on the CG in vitro , it does not appear to be involved directly in the neurite formation elicited by SCM . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| The present data show that in transfected MA 10 ( P+29 ) cells which over express rat phosphodiesterase , the hormone induced stimulation of cAMP and steroid production is directly coupled to receptor activation up to maximal occupation of the LH / CG receptor . ( ABSTRACT TRUNCATED AT 250 WORDS ) . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| This is based , at least in part , on the observations that production of alpha CG is increased by cAMP but not by sodium butyrate in choriocarcinoma cells ( JEG 3 ) , whereas production of alpha CG is increased by butyrate but not by cAMP in cervical carcinoma cells ( HeLa ) . ^^^ Data presented herein confirm that the steady state levels of alpha CG mRNA are increased approximately 10 fold by cAMP in JEG 3 cells , but additionally demonstrate that these levels can be further increased ( to 25 fold ) by sodium butyrate in conjunction with the cyclic nucleotide . ^^^ Similar effects were achieved with the phorbol ester 12 0 tetradecanoylphorbol 13 acetate , which also acted synergistically with cAMP to increase alpha CG mRNA levels ( 24 fold ) . ^^^ Induction of the alpha CG gene by cAMP was independent of protein synthesis , as mRNA levels were comparable when JEG 3 cells were treated with cAMP in the absence and presence of cycloheximide ( CHX ) . ^^^ Together , these results suggest that induction patterns of the alpha CG gene by butyrate and cAMP in trophoblast and nontrophoblast derived tumor cell lines are less distinct than suggested previously and indicate that CHX , which is permissive for induction by butyrate , causes a significant shift in electrophoretic mobility of trans acting factors involved in alpha CG gene expression . . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| Surprisingly , thyrotropin ( TSH ) can increase cAMP and inositol phosphate ( IP ) levels in Cos 7 cells transfected with the lutropin ( LH ) / choriogonadotropin ( CG ) receptor ( LH / CGR ) as well as LH or CG , as evidenced by similar EC 50 and maximal stimulation values . ^^^ After transfection in Cos 7 cells , Mc 2 + 3 + 4 exhibits higher affinity for CG than wild type LH / CGR , but has no CG agonist response in assays measuring cAMP or inositol phosphate ( IP ) levels . ^^^ Conversely , the Mc 1 + 2 chimera exhibits significantly decreased affinity for CG , but CG agonist activity is comparable to wild type LH / CGR in cAMP and IP assays . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| Human chorionic gonadotropin ( CG ) , follicle stimulating hormone ( FSH ) and adrenocorticotropic hormone ( ACTH ) caused significant increases in cyclic monophosphate ( cAMP ) production in tumor tissue in vitro , as compared to controls . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| We also show that LHR C 621 , 622A is capable of binding human CG ( hCG ) and transducing the cAMP signal . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| Recently , we showed that human CG ( hCG ) or phorbol ester [ phorbol 12 myristate 13 acetate ( PMA ) ] stimulation of cells transfected with the LH / CG receptor induced rapid LH / CG receptor phosphorylation and a reduced cAMP response upon reexposure to hCG . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| The production of CG is stimulated by DNA synthesis inhibitors and by cAMP . ^^^ The present study demonstrates that the proto oncogene c jun represses transcription of the human CG alpha and CG beta promoters . c Jun repressed the CG alpha promoter through a canonical cAMP response element ( CRE ) that is known to bind c Jun and other members of the B Zip transcription factor family . ^^^ In the CG beta promoter , two adjacent sites , CRE 1 ( 299 to 289 ) and CRE 2 ( 240 to 219 ) , conveyed cAMP responsiveness via sequences that are distinct from the canonical element , TGACGTCA . ^^^ Thus , both the CG alpha and CG beta genes are repressed by c Jun through promoter regions that convey cAMP induced transcription , although these DNA sequences are unrelated . . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| Thyrotropin , like luteinizing hormone ( LH ) and chorionic gonadotropin ( CG ) , increases cAMP and inositol phosphate levels in cells with recombinant human LH / CG receptor . ^^^ In cells with CGR , about 10 ( 11 ) and 10 ( 9 ) M CG or lutropin ( LH ) significantly increase cAMP and IP levels . ^^^ Surprisingly , however , 10 ( 11 ) and 10 ( 9 ) M TSH are similarly effective in the two assays , respectively , and TSH increases cAMP and IP levels to the same extent as CG and LH . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| LH , FSH , CG and TSH subunits did not stimulate cAMP accumulation up to high concentrations . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| Determination of the intracellular cAMP level by radioimmunoassay revealed a reliable increase of this secondary messenger in splenocyte suppressors during their incubation with CG . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| All substitutions including Arg for Lys 583 did not affect the high affinity human CG binding , but they resulted in the complete loss of cAMP synthesis induced by human CG . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| COS 7 cells were transiently transfected with the eukaryotic expression vector pSVL LH / CG R , wild type and mutants , followed by measurements of human CG binding and human CG mediated cAMP production . ^^^ In summary , these findings indicate a critical role of Tyr 601 in transmembrane signaling of LH / CG R since an Ala replacement results in almost total abolition of cAMP production in response to human CG ; prolines 562 and 591 also appear to be important for full signaling . . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| Media concentrations of cAMP were also elevated with CG treatment and correlated with prorenin values . ^^^ Prorenin secretion induced by CG was markedly attenuated by the cAMP dependent protein kinase inhibitor , H 89 . ^^^ These results indicate that placental prorenin secretion may be locally regulated by CG and mediated by cAMP signal transduction mechanisms . . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| The chosen cells line express comparable densities of cell surface rLHR , bind human CG ( hCG ) with high affinity , and respond to hCG with increases in cAMP and inositol phosphate accumulation . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| Transiently expressed dkFSH R endowed COS 7 cells with both hsLH / CG and FSH binding activity , as well as FSH induced cAMP production . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| In expression studies , mutations of Phe 576 produced functional changes in cAMP and inositol phosphate ( IP ) signaling , and human CG ( hCG ) binding . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| The high dose of CG significantly increased the level of cAMP in the T lymphocytes , and this was associated with a significant suppression of the functional activity of these cells . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| Functional analysis of the LHR mutation , using an in vitro mutagenesis transfection assay , demonstrated complete loss of function , indicated by the lack of cAMP production after human CG stimulation in transfected human embryonic kidney 293 cells . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| Surprisingly , both the adrenal tumors and a cell line ( C alpha 1 ) derived from one of them expressed highly functional LH receptors ( LHR ) , as assessed by Northern hybridization , immunocytochemistry , ligand binding , and human CG ( hCG ) stimulated cAMP and steroid production . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| Basal levels of cAMP in hLHR ( L457R ) expressing cells were , nonetheless , not as great as the levels of cAMP produced by hLHR wild type expressing cells incubated with a saturating concentration of CG . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| These results demonstrate for the first time that the P2UR mRNA is expressed in hGLCs and that P2UR mRNA is regulated by human CG , cAMP , and forskolin . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| The wild type ( WT ) and mutant cDNAs were transiently transfected into COS 7 cells for characterization by human CG ( hCG ) binding and cAMP production . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| An anti EL 2 serum also displays the capacity to inhibit human CG stimulated cAMP production . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| The CG alpha and beta promoters are both responsive to cAMP , although the kinetics of cAMP stimulation are different . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| The production of angiogenin by cultured GCs was stimulated with the addition of human CG or cAMP or under the hypoxic milieu . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| METHODS : Eight male and four female Australian National Team swimmers aged 20 27 yr undertook a 7 10 200 m incremental swimming step test on four occasions over an 8 month period before the 1998 Commonwealth Games ( CG ) : January ( 10 d before the World Championships ) , May ( early season camp ) , July ( midseason ) , and August ( 16 d before the CG ) . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| It has been shown previously that a naturally occurring mutation of the human LH / CG receptor ( hLHR ) , which replaces L 457 in helix 3 with arginine , results in a receptor that constitutively elevates basal cAMP but does not respond to human CG ( hCG ) with further cAMP production . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| The effect of the main pregnancy hormone , chorionic gonadotropin ( CG ) , on proliferation of peripheral blood mononuclear cells ( PBMC ) was studied in the presence of autologous neutrophils ; also , hormone dependent regulation of the cAMP levels in T lymphocytes and neutrophils was evaluated . ^^^ The effect of CG was associated with increased cAMP levels in T lymphocytes and neutrophils . ^^^ Comparison of functional and cAMP related effects of CG in both cell populations indicates that cAMP is involved in the anti proliferative effects of CG . . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| In contrast , FSH and LH / human CG ( via cAMP , PKA , and PI3K pathways ) terminate FKHR expression as granulosa cells differentiate to luteal cells . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| Since both PKA activity and steroidogenic acute regulatory ( StAR ) protein induction are obligatory steps in this transfer of cholesterol , the present study was performed to investigate the role of PTPs in the regulation of PKA activity and StAR expression in response to LH / chorionic gonadotropin ( CG ) and 8Br cAMP in MA 10 cells . ^^^ While the exposure of MA 10 cells to the PTP inhibitor , phenylarsine oxide ( PAO ) , did not modify PKA activity , it partially inhibited the effect of human CG and cAMP analog on StAR protein levels . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| Incubations of BE and HES cells with CG did not significantly alter adenylyl cyclase activity or increase intracellular cAMP when compared with Chinese hamster ovarian cells stably transfected with the full length human CG / luteinizing hormone ( LH ) receptor ( CHO LH cells ) . ^^^ In conclusion , immediate signal transduction pathways induced by CG in endometrial epithelial cells are cAMP independent and stimulate phosphorylation of ERK 1 / 2 via a MEK 1 / 2 pathway , leading to an increase in PGE ( 2 ) release as the possible result of cyclooxygenase 2 activation . . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| Thus luteinized cells , despite increased gene transcripts to LH / chorionic gonadotrophin ( CG ) receptors , respond inefficiently to gonadotrophin stimulation , due to attenuation of signal transduction in the cAMP cascade at multiple steps . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| RESULTS AND CONCLUSIONS : None of the immunoglobulin G ( IgG ) preparations from patients with POF was able to inhibit the activity of the FSH and CG stimulated cAMP production . . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| Stimulation of the COS 7 cell line with equipotent concentrations of CG and CG ( CTP ) resulted in significantly different formation of cAMP ( two way ANOVA , p < 0 . 001 ) . ^^^ In particular , cAMP production stimulated by CG ( CTP ) was 3 4 times lower compared to CG at the saturating CG concentration ( 8 IU / L ) . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| Inhibition analysis employing blockers of cAMP dependent protein kinase ( H 89 ) and L type calcium channels ( verapamil ) as well as an antagonist of progesterone nuclear receptors ( RU 486 ) demonstrated that the inhibitory ( for IFNgamma ) and stimulatory ( for IL 4 ) effects of CG were mediated by cAMP , while the effects of steroid hormones on the production of these cytokines were realized through genomic and non genomic mechanisms ( the latter mechanisms were largely mediated by L type calcium channel regulation ) . ^^^ |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P49913 and P08311 |
Pubmed |
SVM Score :0.0 |
| NA |
|