| Interacting proteins: Q99828 and P49810 |
Pubmed |
SVM Score :1.0257948 |
| The EF hand calcium binding protein Calmyrin ( also called CIB 1 ) was shown to interact with presenilin 2 ( PS 2 ) , suggesting that this interaction might play a role in the pathogenesis of Alzheimer ' s disease ( AD ) . 1.0257948^^^ |
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| Interacting proteins: Q99828 and P49810 |
Pubmed |
SVM Score :0.0 |
| Here , we show that NBR 1 interacts with two proteins ; fasciculation and elongation protein zeta 1 ( FEZ 1 ) , a PKCzeta interacting protein , and calcium and integrin binding protein ( CIB ) , which is associated with polo like kinases Fnk / Snk and the Alzheimer ' s disease presenilin 2 protein . ^^^ |
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| Interacting proteins: Q99828 and P49810 |
Pubmed |
SVM Score :0.0 |
| By two hybrid screening , we found that 6 16 protein interacts with calcium and integrin binding protein , CIB / KIP / Calmyrin ( CIB ) , which interacts with presenilin 2 , a protein involved in Alzheimer ' s disease . ^^^ |
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| Interacting proteins: Q99828 and P49810 |
Pubmed |
SVM Score :0.0 |
| We report here the identification of a calcium binding protein , calmyrin , that interacts preferentially with presenilin 2 ( PS 2 ) . ^^^ Calmyrin is myristoylated , membrane associated , and colocalizes with PS 2 when the two proteins are overexpressed in HeLa cells . ^^^ Yeast two hybrid liquid assays , affinity chromatography , and coimmunoprecipitation experiments confirm binding between PS 2 and calmyrin . ^^^ Functionally , calmyrin and PS 2 increase cell death when cotransfected into HeLa cells . ^^^ |
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| Interacting proteins: Q99828 and P49810 |
Pubmed |
SVM Score :0.0 |
| Calcium binding sequences in calmyrin regulates interaction with presenilin 2 . ^^^ Calmyrin interacts with a number of proteins , including presenilin 2 ( PS 2 ) . ^^^ By yeast two hybrid assays , we found that both EF hands 3 and 4 of calmyrin must be intact for calmyrin to interact with PS 2 loop sequences . ^^^ Double immunofluorescence microscopy indicated that Wt and myristoylation defective calmyrin proteins colocalize efficiently and to the same extent with PS 2 , whereas calmyrin mutants defective in calcium binding display less colocalization with PS 2 . ^^^ Our results suggest that calmyrin functions as a calcium sensor and that calcium binding sequences in calmyrin are important for interaction with the PS 2 loop . . ^^^ |
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| Interacting proteins: Q99828 and P49810 |
Pubmed |
SVM Score :0.0 |
| Ca2+ independent binding and cellular expression profiles question a significant role of calmyrin in transduction of Ca2+ signals to Alzheimer ' s disease related presenilin 2 in forebrain . ^^^ The interaction between the EF hand Ca ( 2+ ) binding protein calmyrin and presenilin 2 ( PS 2 ) has been suggested to play a role in Alzheimer ' s disease ( AD ) . ^^^ We now report that calmyrin binds specifically endogenous PS 2 and not PS 1 . ^^^ Finally , subcellular fractionation demonstrates only a limited overlap of calmyrin and PS 2 at neuronal membranes . ^^^ We therefore conclude that calmyrin will not contribute significantly as a Ca ( 2+ ) sensor that transduces Ca ( 2+ ) signaling events to PS 2 in forebrain . . ^^^ |
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