| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| Based on the finding that area A was bound by a liver selective factor ( s ) related to CCAAT / enhancer binding protein ( C / EBP ) , we performed cotransfection assay and showed that C / EBP family members and a related factor , albumin D element binding protein ( DBP ) stimulate transcription from the arginase promoter . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| Two other footprinted sites correspond to binding sites for the liver transcription factors C / EBP and albumin D element binding protein and a third to an HNF 1 binding site . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| As part of the long term goal to investigate the role of LETFs in GH regulation of gene expression in the liver , in this study we determined the effect of GH administration on the expression of 10 LETFs , including hepatocyte nuclear factor ( HNF ) 1alpha , HNF 1beta , HNF 3alpha , HNF 3beta , HNF 3gamma , HNF 4alpha , HNF 6 , CCAAT / enhancer binding protein ( C / EBP ) alpha , C / EBPbeta , and albumin D element binding protein ( DBP ) in the bovine liver . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| These include the hepatocyte nuclear factors 1 , 3 , 4 ( HNF 1 , 3 , 4 ) , some members of the CAAAT / enhancer binding protein ( C / EBP ) family , and D site binding protein ( DBP ) . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| The mRNA for albumin and D site binding protein ( DBP ) was more abundant and mRNA for CCAAT / enhancer binding protein beta ( C / EBP beta ) was less abundant in the cyclic parenteral nutrition group than in the continuous parenteral nutrition group . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| Among nuclear factors binding to the D site of albumin gene promoter , the CCAAT / enhancer binding protein alpha ( C / EBP alpha ) mRNA level showed a decrease and the D site binding protein ( DBP ) mRNA level tended to decrease after 3 d of starvation . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| We hypothesize that the transcription factors C / EBP and DBP are the principal tissue specific regulators in liver , and that HNF 1 and perhaps C / EBP are important for kidney specific PEPCK expression . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| Both recombinant DBP and C / EBP bind with highest affinity to regions located at positions 85 and 245 in the promoter , but display differences in their binding properties at other sites . ^^^ Using eukaryotic expression vectors for both C / EBP and DBP , we found that with 5 ' deletion mutants of the PEPCK promoter , both C / EBP and DBP exerted their effects through similar regions of the promoter . ^^^ However , the use of internal deletion mutants of the promoter identified distinct differences in the mechanism of activation by C / EBP and DBP . ^^^ In particular , a region of the promoter between positions 86 and 117 significantly attenuated the level of trans activation by DBP , but not by C / EBP . ^^^ Evidence presented also supports a model whereby the relative ratios of C / EBP and DBP in the cell fine tune the expression of the PEPCK gene . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| The sequence of this region bears significant similarity to binding sites of members of the bZIP family of liver enriched or specific factors such as C / EBP , DBP , and LAP , that are characteristically produced relatively late during liver development . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| They comprise recognition sites for the oestrogen receptor , the ubiquitous COUP transcription factor , the liver enriched C / EBP and / or DBP and the liver specific LF A 1 . ^^^ In vitro , binding of protein to the oestrogen response element ( ERE ) is excluded by the prior binding of a protein , possibly C / EBP or DBP , to an adjacent element . ^^^ The results suggests that oestrogen dependent liver specific activation of the apoVLDL 2 promoter is established by the binding of the oestrogen receptor to EREs and multiple liver enriched factors ( C / EBP , DBP and LF A 1 ) to their nearby recognition sequences . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| DBP belongs to a family of related transcription factors including Fos , Jun , CREB , and C / EBP , which share a conserved basic domain . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| Chromosomal localization in man and rat of the genes encoding the liver enriched transcription factors C / EBP , DBP , and HNF1 / LFB 1 ( CEBP , DBP , and transcription factor 1 , TCF 1 , respectively ) and of the hepatocyte growth factor / scatter factor gene ( HGF ) . ^^^ By means of somatic cell hybrids segregating either human or rat chromosomes , we determined the chromosome localization of three genes encoding transcription factors expressed in hepatocytes , namely , C / EBP ( CCAAT / enhancer binding protein ) , DBP ( D site of albumin promoter binding protein ) , and HNF1 / LFB 1 ( designated transcription factor 1 , gene symbol : TCF 1 ) , and of the hepatocyte growth factor gene , which is identical to the mitogenic and chemotactic factor designated scatter factor ( gene symbol : HGF ) . ^^^ The CEBP and DBP genes , encoding two related transcription factors , were found to be syntenic both on human chromosome 19 and on rat chromosome 1 . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| C / EBP , the liver enriched factor , specifically interacts with site 1 whereas its related proteins ( e . g . ; DBP , LAP / NFIL6 ) most likely recognize sites 2 and 3 . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| We examined the role of upstream DNA and liver specific transcription factors in regulation of this promoter by use of transient transfection of heterologous chloramphenicol acetyltransferase gene constructs and vectors containing cDNAs encoding the liver enriched transcription factors HNF 1 alpha , C / EBP , and DBP . ^^^ Interestingly , the DBP binding regions of both CYP2C6 and albumin bind to C / EBP , but this factor is capable of transactivating only the latter gene . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| For all three promoters , the activation by LAP or DBP was higher than that seen by HNF 1 or C / EBP alpha , and a significant synergism between C / EBP alpha and LAP was noticed for the ADH 2 and ADH 3 promoters when both factors were simultaneously cotransfected . ^^^ In all three ADH genes , LAP bound to the same four sites previously reported for C / EBP alpha ( i . e . , 160 , 120 , 40 , and 20 bp ) , but DBP bound strongly only to the site located at 40 bp relative to the transcriptional start . ^^^ These studies suggest that HNF 1 and C / EBP alpha help establish ADH gene family transcription in fetal liver and that LAP and DBP help maintain high level ADH gene family transcription in postnatal liver . . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| Developmental changes in the expression of the liver enriched transcription factors LF B 1 , C / EBP , DBP and LAP / LIP in relation to the expression of albumin , alpha fetoprotein , carbamoylphosphate synthase and lactase mRNA . ^^^ We investigated by in situ hybridization whether this set of transcription factors , LF B 1 , C / EBP , DBP and LAP / LIP , is expressed coordinately in the liver during embryonic development and to what extent they are also expressed elsewhere . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| C / EBP alpha , C / EBP beta and D binding protein ( DBP ) acted as trans activators on the AFP promoter , whereas liver inhibitory protein ( LIP ) , a truncated form of C / EBP beta , was a potent negative regulator of the promoter . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| Since liver tissue contains high levels of the bZIP factors , liver enriched activating protein ( LAP ) , the CCAAT / enhancer binding protein alpha ( C / EBP alpha ) , and the D element binding protein ( DBP ) , and since P 1 contains a functional C / EBP alpha binding site ( P 1 CBS ) , we have examined the role of these transcription factors in the activation of IGF 2 P 1 . ^^^ Transient transfection experiments reveal that P 1 can be activated up to 15 fold by LAP and up to 6 fold by C / EBP alpha but can not be activated by DBP . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| In this study , the functional role of DBP and C / EBP ( which have several potential binding sites on the IGFBP 1 proximal promoter ) have been investigated . ^^^ Transient co transfection of the reporter plasmid , pBP 1341 and eukaryotic expression vectors which code for DBP and C / EBP in human cell lines Hep3B , HepG 2 and C 33 showed that IGFBP 1 promoter activity was unchanged by C / EBP , but increased between 2 and 7 times by DBP ( depending on the cell line ) . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| Only HNF 1 alpha , and not HNF 1 beta , HNF 4 , C / EBP alpha , C / EBP beta , or DBP , was able to activate the CYP2E1 promoter . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| Aldolase B gene expression appeared restricted to the proximal tubule , the site where HNF 1 alpha , HNF 1 beta , C / EBP alpha , and DBP transcripts were also abundant . ^^^ Transactivation experiments in PKSV PCT cultured cells showed that HNF 1 alpha , C / EBP alpha , and DBP behave as transactivators of the 190 bp aldolase B gene promoter , and that HNF 1 beta had a low transactivating efficiency . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| Co transfection of expression plasmids encoding C / EBP alpha or a related leucine zipper factor D binding protein ( DBP ) revealed a C / EBP specific increase in reporter gene activity in HepG 2 cells transfected with caeruloplasmin chloramphenicol acetyltransferase containing the 93 to 48 region . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| To better understand the regulation of this process we have studied the expression of liver enriched transcriptional factors ( HNF 1 alpha and HNF 1 beta , HNF 3 alpha , HNF 3 beta , and HNF 3 gamma , HNF 4 , C / EBP , C / EBP beta , and DBP ) in an experimental model of oval cell proliferation and differentiation and compared the expression of these factors to that observed during late stages of hepatic ontogenesis . ^^^ The steady state mRNA levels of four ( HNF 1 alpha , HNF 3 alpha , HNF 4 , and C / EBP beta ) `` liver enriched ' ' transcriptional factors gradually decrease during the late period of embryonic liver development while three factors ( HNF 1 beta , HNF 3 beta , and DBP ) increase . ^^^ However , during early stages of oval cell proliferation both small and large bile ducts start to express HNF 1 alpha and HNF 1 beta , HNF 3 gamma , C / EBP , and DBP but not HNF 4 . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| Competition for CYP 7 promoter binding sites between C / EBP , which undergoes < or = 2 fold diurnal change in rat liver , and the diurnally regulated DBP is proposed to determine the relative rates of basal versus diurnally regulated CYP 7 gene transcription and thus may be a primary mechanism for setting the 3 6 fold amplitude that characterizes the circadian rhythm of liver CYP 7 expression . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| Activity was markedly increased by cotransfection with a vector expressing C / EBP beta but was unaffected by vectors producing other liver enriched transcription factors ( C / EBP alpha , HNF 1 alpha , and DBP ) . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| This region contains the following regulatory elements : nitrogen regulatory / oxygen sensing consensus sequences , 5 ' TTTTGCA and 5 ' CCCTGCA ; tissue specific regulatory elements , including binding sites for A activator , 5 ' GTGGTGCAA ; for DBP , 5 ' TGATTTTGT ; for HNF , 5 ' T ( A / G ) TTTGT ; and for C / EBP , 5 ' T ( T / G ) ( T / G ) TGCAAT ; a lymphokine responsive element , 5 ' GTGAAACCCC ( Rev ) , as well as binding sites for AP and Sp 1 . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| One of the members of the bZIP family of transcriptional activators is NF IL6 / LAP ( IL 6 DBP , C / EBP beta , CRP 2 ) . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| Competition between transcription factors HNF 1 and HNF 3 , and alternative cell specific activation by DBP and C / EBP contribute to the regulation of the liver specific aldolase B promoter . ^^^ From 5 ' to 3 ' , we found : a negative DE element , an activating C / EBP DBP binding site , a CCAAT box binding NFY that seems to play a negative role , and an activating element consisting of two overlapping binding sites for HNF 1 and HNF 3 . ^^^ We found that DBP and HNF 1 are strong transactivators of the aldolase B promoter while C / EBP and vHNF 1 are only weak activators and HNF 3 alone does not modify such activity . ^^^ Deletion of the distal negative element results in a similar transactivation by C / EBP and DBP , enhanced for the former and reduced for the latter . ^^^ In hepatocytes in primary culture , the strong transactivator is C / EBP while DBP is essentially inactive . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| C / EBP , LAP , DBP , HNF 3 , and vHNF 1 expression are not systematically extinguished in parallel with the hepatic functions . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| Synergy between transcription factors DBP and C / EBP compensates for a haemophilia B Leyden factor 9 mutation . ^^^ The correlation between the post pubertal recovery of these mutants and the induction of the transcription factor DBP led us to the discovery of a synergistic interaction between DBP and C / EBP responsible for the recovery of normal transcriptional activity of the 5 mutant promoter and may play a role in the resolution of other Leyden mutants . . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| The nucleotide motifs located between nt 108 and 121 of the GRU , interacted with the HNF 3 family of trans acting factors ( alpha , beta , gamma ) , whereas those encompassing nt 81 to 104 bound DBP and / or nuclear proteins sharing similar sequence specificity ( i . e . from the C / EBP family of bZIP proteins ) . ^^^ In both cases , the mechanism would involve the interplay of common trans acting factor ( s ) , some of which are liver enriched [ HNF 3 , DBP or C / EBP related bZIP proteins ] with their cognate target sequence . . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| By using a PCR based amplification assay we selected high affinity DNA binding sites for C / EBP beta and the PAR protein DBP from a pool of oligonucleotides . ^^^ C / EBP beta 5 > A selectively binds only the subset of C / EBP sites that are also DBP sites , both as oligonucleotides and within the natural contexts of the albumin and cholesterol hydroxylase promoters . . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| Role of the liver enriched transcription factors C / EBP alpha and DBP in the expression of human CYP3A4 and CYP3A7 . ^^^ The aim of the present work was to make a functional analysis of the 5 ' flanking regions of CYP3A4 and CYP3A7 in different cell lines , including CHO , HepG 2 , WRL 68 and Caco 2 TC 7 , after cotransfection with two hepato specific transcription factors , C / EBP alpha and DBP . ^^^ Although preliminary , our results suggest that C / EBP alpha , and possibly other members of the C / EBP family , play a prominent part in the expression of the CYP3A family in man , and that the two genes respond differently to C / EBP alpha and DBP , two factors that exhibit a strict proliferation dependent pattern of expression in the liver . . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| This paper examines the regulation of the DBP promoter by C / EBP alpha and examines the role of autoregulation in its expression . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| Serum albumin and albumin mRNA levels in the PN group became significantly decreased compared to those in the OR group , whereas the EN group did not show a significant difference compared to the OR group . mRNA levels of DBP , D site binding transcription factor for the albumin gene , showed a circadian rhythm in the OR and EN groups , but not in the PN group , as indicated by a DBP mRNA level at 2200 h in the PN group that was significantly lower than that in the OR group . mRNA levels of C / EBP alpha , CCAAT enhancer binding protein alpha , were highest in the OR group and lowest in the PN group . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| Analysis of the expression of relevant hepatic genes by reverse Northern and semiquantitative reverse transcriptase polymerase chain reaction ( RT PCR ) revealed that culturing hepatocytes in collagen gels results in a sustained higher expression of key liver transcription factor genes DBP , C / EBP alpha and beta , and HNF 1 and 4 , as well as specific liver enzyme genes ( phosphoenol pyryvate carboxykinase , and carbamoylphosphate synthetase 1 ) . . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| By analysing DNA sequences implicated in liver specific transcription , it has been possible to identify members of the nuclear proteins , such as the liver enriched transactivating factors , hepatic nuclear factor 1 ( HNF 1 ) , HNF 3 , HNF 4 , HNF 6 , CCAAT / enhancer binding protein ( C / EBP ) , and D binding protein ( DBP ) , which are key elements in the liver specific transcriptional regulation of genes . ^^^ |
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| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| We also show that co transfection of DBP ( which is a member of C / EBP family of transcription factors ) increases promoter activity of these reporter constructs . . ^^^ |
|
| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q10586 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
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