| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| A 32P labeled LAP DNA binding and dimerization domain `` zipper probe ' ' was used to isolate a clone that encodes a new C / EBP homologous protein : CHOP 10 . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| However , C / EBP homologous protein ( CHOP 10 ) mRNA levels markedly increase in growth arrested NIH3T3 cells . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| The simultaneous transfection of the C / EBP homologous protein ( CHOP ) ( also known as growth arrest DNA damage protein 153 or gadd 153 ) inhibited this C / EBP dependent activation in a concentration dependent manner . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| The transcription factor CHOP ( C / EBP homologous protein 10 ) is a bZIP protein induced by a variety of stimuli that evoke cellular stress responses and has been shown to arrest cell growth and to promote programmed cell death . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| We studied the expression of C / EBP homologous protein ( CHOP ) and asparagine synthetase ( AS ) in response to deprivation of a single AA and investigated the possible link between protein synthesis inhibition due to amino acid limitation and gene expression . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| For instance , limitation of several amino acids greatly increases the expression of the genes encoding insulin like growth factor binding protein 1 , CHOP ( C / EBP homologous protein , where C / EBP is CCAAT / enhancer binding protein ) and asparagine synthetase . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Role of C / EBP homologous protein ( CHOP 10 ) in the programmed activation of CCAAT / enhancer binding protein beta during adipogenesis . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| According to both transfection and DNA binding analyses , liver enriched inhibitory protein ( LIP ) , the shorter form of C / EBPbeta and C / EBP homologous protein 10 ( CHOP 10 ) , were found to inhibit C / EBPdelta gene expression . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Induction of the C / EBP homologous protein ( CHOP ) by amino acid deprivation requires insulin like growth factor 1 , phosphatidylinositol 3 kinase , and mammalian target of rapamycin signaling . ^^^ Here , we examined the signaling pathways involved in the induction of the C / EBP homologous protein ( CHOP ) by amino acid starvation . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| CHOP , a C / EBP homologous protein that is induced by endoplasmic reticulum ( ER ) stress and plays a role in growth arrest and cell death , was induced by a NO donor , S nitroso N acetyl D , L penicillamine ( SNAP ) . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| We found that C / EBPbeta induces neuronal differentiation and that this process is inhibited by transfection with the C / EBP homologous protein 10 ( CHOP ) , strongly suggesting that the extension of neurites is indeed due to the C / EBPbeta transcriptional activity . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Genistein inhibits CCAAT / enhancer binding protein beta ( C / EBPbeta ) activity and 3T3 L 1 adipogenesis by increasing C / EBP homologous protein expression . ^^^ We found that genistein blocked the DNA binding and transcriptional activity of CCAAT / enhancer binding protein beta ( C / EBPbeta ) during differentiation by promoting the expression of C / EBP homologous protein , a dominant negative member of the C / EBP family . ^^^ Collectively , these results indicate that genistein blocks adipogenesis and C / EBPbeta activity by increasing the level of C / EBP homologous protein and possibly by inhibiting the tyrosine phosphorylation of C / EBPbeta . . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| To test this , we used HEK 293 cells stably cointegrated with GAL 4 transcriptional activation systems of Elk 1 , c Jun or C / EBP homologous protein ( CHOP ) . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Human myxoid liposarcoma contains a characteristic t ( 12 ; 16 ) chromosomal translocation that results in fusion of the N terminal domain of the translocated in liposarcoma ( TLS ) protein to the C / EBP homologous protein ( CHOP ) . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| The cells selectively increased phosphorylation of p 38 MAPK , upstream dual activator MAPK kinase 3 / 6 , and downstream transcription factors activating transcription factor 2 , ETS domain transcription factor 1 , and C / EBP homologous protein in a time dependent manner . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| C / EBP homologous protein ( CHOP ) up regulates IL 6 transcription by trapping negative regulating NF IL 6 isoform . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| ER stress stimuli induced expressions of Bip ( binding Ig protein ) and CHOP ( C / EBP homologous protein ) in MEF of wild type mice . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| The human , but not the mouse , promoter also binds C / EBP homologous protein ( CHOP ) , which competes with STAF for the same binding site . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Moreover , edaravone suppressed ER stress mediated apoptosis by inhibiting eukaryotic initiation factor alpha phosphorylation , C / EBP homologous protein ( CHOP ) induction , and caspase 12 activation . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Palmitate and oleate induced C / EBP homologous protein , activating transcription factor 4 , and immunoglobulin heavy chain binding protein mRNAs , 10 box binding protein 1 alternative splicing , and activation of the activating transcription factor 6 promoter in INS 1E cells , suggesting that FFAs trigger an endoplasmic reticulum ( ER ) stress response . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Retinoic acid regulates C / EBP homologous protein expression ( CHOP ) , which negatively regulates myeloid target genes . ^^^ In this study , we have identified the C / EBP homologous protein ( CHOP ) as a novel retinoid responsive gene using a polymerase chain reaction ( PCR ) based cDNA subtraction method . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| The transcription factor C / EBP homologous protein ( CHOP ) is activated by ER stress , and CHOP deletion protects against its lethal consequences . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND AND OBJECTIVES : C / EBP homologous protein ( CHOP ) is an endoplasmic reticulum ( ER ) stress inducible transcription factor involved in the development of apoptosis , growth arrest , and differentiation . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Also , like the ER stressors , NE not only increased phosphorylation of both the alpha subunit of eukaryotic initiation factor 2 and its upstream RNA dependent protein kinase like ER kinase over 12 h of treatment but also increased ER chaperone molecule glucose regulated protein 78 and the nuclear transcription factor C / EBP homologous protein . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Prostaglandin E 2 induces interleukin 8 gene transcription by activating C / EBP homologous protein in human T lymphocytes . ^^^ Here , we demonstrate that , in human T cells , PGE ( 2 ) induced IL 8 mRNA transcription through prostaglandin E ( 2 ) receptors 1 and 4 dependent signal transduction pathways leading to the activation of the transcription factor C / EBP homologous protein ( CHOP ) , never before implicated in IL 8 transcription . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| In this study , we demonstrate that the progressive replication of Tula virus in Vero E 6 cells initiates several death programs that are intimately associated with ER stress : ( 1 ) early activation of ER resident caspase 12 ; ( 2 ) phosphorylation of Jun NH 2 terminal kinase ( JNK ) and its downstream target transcriptional factor , c jun ; ( 3 ) induction of the pro apoptotic transcriptional factor , growth arrest and DNA damage inducible gene 153 , or C / EBP homologous protein ( Gadd153 / chop ) ; and ( 4 ) changes in the ER membrane protein BAP 31 implying cross talk with the mitochondrial apoptosis pathway . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| C / EBP homologous protein ( CHOP ) is a stress inducible nuclear protein that is crucial for the development of programmed cell death and regeneration ; however , the regulation of its function has not been well characterized . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| We found that SNP induced C / EBP homologous protein ( CHOP ) expression , which inhibited C / EBP binding activity and reduced the C / EBP activity induced by chronic activation of PI 3 kinase in a dose dependent manner up to 1 . 0 mM . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| We have studied the activation of the UPR in VWM through the immunohistochemical expression of its upstream components PERK and phosphorylated eIF2alpha ( eIF2alphaP ) and combined immunohistochemical and Western blot analysis of the downstream effector proteins activating transcription factor 4 ( ATF 4 ) and C / EBP homologous protein ( CHOP ) in 4 VWM brains and 3 age matched controls . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| ER induced apoptosis involves the activation of caspase 12 and C / EBP homologous protein ( CHOP ) , and the shutdown of translation initiated by phosphorylation of eIF2alpha . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| A likely cause of macrophage death is accumulation of free cholesterol ( FC ) in the ER , leading to activation of the unfolded protein response ( UPR ) and C / EBP homologous protein ( CHOP ) induced apoptosis . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| C / EBP homologous protein is necessary for normal osteoblastic function . ^^^ C / EBP homologous protein ( CHOP ) suppresses adipogenesis and accelerates osteoblastogenesis in vitro . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| The p 53 independent apoptotic pathway may be mediated by C / EBP homologous protein ( CHOP ) and caspase 12 , as their activation is intact in p 53 ( / ) MEFs . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| The C / EBP homologous protein CHOP ( GADD 153 ) is an inhibitor of Wnt / TCF signals . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| In the present study , we show that caspase 8 activation in these cells is mediated through ER stress associated elevation of death receptor DR 5 and the C / EBP homologous protein ( GADD153 / CHOP ) , an ER stress initiated transcription factor , rather than through TNFalpha and TNF R 1 interactions on the cell surface . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Compared to wild type bi 1 mice , bi 1 knockout mice subjected to hepatic IR injury exhibited these characteristics : ( 1 ) increased histological injury ; ( 2 ) increased serum transaminases , indicative of more hepatocyte death ; ( 3 ) increased percentages of TUNEL positive hepatocytes ; ( 4 ) greater elevations in caspase activity ; and ( 5 ) more activation of ER stress proteins inositol requiring enzyme 1 and activating transcription factor 6 and greater increases in expression of ER stress proteins C / EBP homologous protein and spliced XBP 1 protein . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Furthermore , arylating quinones induced endoplasmic reticulum ( ER ) stress by activating the pancreatic ER kinase ( PERK ) signaling pathway including elF2alpha , ATF 4 , and C / EBP homologous protein ( CHOP ) . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| There were no differences in protein expression of the pro apoptotic factor C / EBP homologous protein ( CHOP ) or the endoplasmic reticulum ( ER ) chaperone binding Ig protein . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| In CGN , a significant increase in the expression of C / EBP homologous protein ( CHOP ) protein was detected after exposure to Abeta or TM , whereas no such an increase in the protein expression was observed in HPN . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Curcumin also induced the expression of a potential pro apoptotic gene , C / EBP homologous protein ( CHOP ) , both at its mRNA and protein levels . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| C / EBP homologous protein ( CHOP ) is crucial for the induction of caspase 11 and the pathogenesis of lipopolysaccharide induced inflammation . ^^^ C / EBP homologous protein ( CHOP ) / growth arrest and DNA damage inducible gene 153 is a C / EBP family transcription factor which is involved in endoplasmic reticulum ( ER ) stress mediated apoptosis . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Here we show that C / EBP homologous protein ( CHOP ; also known as C / EBPzeta ) is expressed in bone as well as in mesenchymal progenitors and primary osteoblasts . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| A comparison with M , E , and NSP 6 proteins indicates that SARS CoV spike ( S ) protein sufficiently induces transcriptional activation of several UPR effectors , including glucose regulated protein 78 ( GRP 78 ) , GRP 94 , and C / EBP homologous protein . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| We have used Southern blot technique to test whether a gene of the CCAAT / enhancer binding protein ( C / EBP ) family , CHOP , which maps to 12q13 and is assumed to be involved in adipocyte differentiation , could be the 12q gene in question . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| The growth arrest and DNA damage inducible gene CHOP ( GADD 153 ) encodes a small nuclear protein from the C / EBP family , originally isolated from adipocytes in culture . ^^^ CHOP dimerizes with other C / EBP proteins and the CHOP C / EBP dimers are directed away from `` classical ' ' C / EBP sites recognizing instead unique `` nonclassical ' ' sites . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Human myxoid liposarcomas contain a characteristic chromosomal translocation , t ( 12 ; 16 ) ( q 13 ; p 11 ) , that is associated with a structural rearrangement of the gene encoding CHOP , a growth arrest and DNA damage inducible member of the C / EBP family of transcription factors residing on 12q13 . 1 . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| CHOP , a member of the C / EBP family of transcription factors , mediates effects of cellular stress on growth and differentiation . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| CHOP ( GADD 153 ) is a mammalian nuclear protein that dimerizes with members of the C / EBP family of transcriptional factors . ^^^ The basic region of CHOP deviates considerably in sequence from that of other C / EBP proteins , and CHOP C / EBP heterodimers are incapable of binding to a common class of C / EBP sites . ^^^ With respect to such sites , CHOP serves as an inhibitor of the activity of C / EBP proteins . ^^^ In this study an in vitro PCR based selection assay was used to identify sequences bound by CHOP C / EBP dimers . ^^^ We conclude that CHOP may serve a dual role both as an inhibitor of the ability of C / EBP proteins to activate some target genes and as a direct activator of others . . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| GADD153 / CHOP is a DNA damage inducible , nuclear leucine zipper protein that is capable of producing a G 1 S arrest in fibroblastic cells and of dimerizing with and inhibiting CAAT / enhancer binding protein ( C / EBP ) activities . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Similarly , preincubation with CHOP , a truncated member of the C / EBP family which contains a beta leucine zipper domain , does not prevent or alter the mobility of this novel DNA / protein complex , indicating that components of this complex do not form heterodimers with beta ZIP proteins . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| CHOP ( GADD 153 ) is a member of the C / EBP family and a stress induced protein . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Here we examined the role of amino acid limitation in regulating the expression of CHOP , a CCAAT / enhancer binding protein ( C / EBP ) related gene . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Transcription factors C / EBP alpha , C / EBP beta , and CHOP ( Gadd 153 ) expressed during the differentiation program of keratinocytes in vitro and in vivo . ^^^ Within this framework , western immunoblot analysis and immunohistochemistry reveal that C / EBP alpha increases 5 fold at 1 2 d and remains elevated , C / EBP beta rises 2 fold at 2 4 d and gradually falls , and CHOP rises 9 fold in the first 24 h then returns rapidly to baseline . ^^^ C / EBP alpha is concentrated in the upper epidermis in a predominantly cytoplasmic location within cells , whereas the highest levels of C / EBP beta and CHOP are seen in the mid epidermis , mainly within nuclei . ^^^ High levels of C / EBP beta and CHOP ( but not C / EBP alpha ) are also observed in hair follicles and sebaceous glands . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Human translocation liposarcoma CCAAT / enhancer binding protein ( C / EBP ) homologous protein ( TLS CHOP ) oncoprotein prevents adipocyte differentiation by directly interfering with C / EBPbeta function . ^^^ Human translocation liposarcoma ( TLS ) CCAAT / enhancer binding protein ( C / EBP ) homologous protein ( CHOP ) is a fusion oncoprotein found specifically in a malignant tumor of adipose tissue and results from a t ( 12 ; 16 ) translocation that fuses the amino terminal part of TLS to the entire coding region of CHOP . ^^^ Being that CHOP is a member of the C / EBP transcription factor family , proteins that comprise part of the adipocyte differentiation machinery , we examined whether TLS CHOP blocked adipocyte differentiation by directly interfering with C / EBP function . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| CHOP ( GADD 153 ) is a small nuclear protein that dimerizes avidly with members of the C / EBP family of transcription factors . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| CHOP ( also called GADD 153 ) is a stress inducible nuclear protein that dimerizes with members of the C / EBP family of transcription factors and was initially identified as an inhibitor of C / EBP binding to classic C / EBP target genes . ^^^ Subsequent experiments suggested a role for CHOP C / EBP heterodimers in positively regulating gene expression ; however , direct evidence that this is the case has so far not been uncovered . ^^^ Here we describe the identification of a positively regulated direct CHOP C / EBP target gene , that encoding murine carbonic anhydrase 6 ( CA 6 ) . ^^^ A CHOP responsive element was mapped to the inducible CA 6 promoter , and in vitro footprinting revealed binding of CHOP C / EBP heterodimers to that site . ^^^ Rescue of CA 6 expression in c / ebpbeta / cells by exogenous C / EBPbeta and a shorter , normally inhibitory isoform of the protein known as LIP suggests that the role of the C / EBP partner is limited to targeting the CHOP containing heterodimer to the response element and points to a preeminent role for CHOP in CA 6 induction during stress . . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Gadd 153 , also known as chop , encodes a member of the CCAAT / enhancer binding protein ( C / EBP ) transcription factor family and is transcriptionally activated by cellular stress signals . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Until now , however , the only activity attributed to the oncoprotein is that of inhibiting the binding of transcription factors of the C / EBP class to certain adipogenic target genes , a function that TLS CHOP shares with the nononcogenic CHOP protein . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| The results strongly suggest that CHOP plays a role in erythropoiesis , possibly through interactions with both C / EBP and non C / EBP family members . . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| The major active promoter element was localized by nested deletions and mutations to a DNA element ( TGCAAT , 59 to 54 ) that presumptively binds the transcription factor CHOP [ CAAT enhancer binding protein ( C / EBP ) homologous protein ] . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| The transcription factor CHOP ( GADD 153 ) heterodimerizes with other C / EBP family members , especially C / EBPbeta , thus preventing their homodimerization and binding to DNA sequences specific for the homodimers . ^^^ Some CHOP C / EBP heterodimers apparently bind to alternative DNA sequence and thereby regulate the transcription of other genes . ^^^ In the present study , we report that CHOP also interacts with another non C / EBP protein designated 5 fos transformation effector ( FTE ) ( Kho , C . ^^^ FTE / S3a appears to be a bifunctional ribosomal protein that regulates CHOP and , hence , C / EBP function during erythropoiesis . . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Here we examined the molecular mechanisms involved in the regulation of CHOP ( a CCAAT / enhancer binding protein [ C / EBP ] related gene ) expression upon amino acid limitation . ^^^ In addition , we show that the CHOP AARE is related to C / EBP and ATF / CRE binding sites and binds in vitro the activating transcription factor 2 ( ATF 2 ) in starved and unstarved conditions . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Both GH and BAPTA AM caused a rapid induction of the transcription factor C / EBP homology protein ( CHOP ) and an additive effect was observed with combined treatment , which suggests a regulatory role of GH on endoplasmic reticulum stress . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Under these conditions , we found that mRNA and protein for CHOP / GADD153 , a C / EBP family transcription factor which is involved in endoplasmic reticulum ( ER ) stress induced apoptosis , are induced . iNOS mRNA was induced 2 h after treatment , whereas CHOP mRNA began to increase at 6 h with a time lag . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Chop is a ubiquitously expressed mammalian gene encoding a small nuclear protein related to the CCAAT / enhancer binding protein ( C / EBP ) family of transcription factors . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| CHOP in 12q13 , also called GADD 153 or DDIT 3 , encodes a transcription factor of the C / EBP type . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Here , we identified the basic leucine zipper transcription factor , the growth arrest and DNA damage 153 ( GADD 153 ) / CCAAT enhancer binding protein ( C / EBP ) homologous protein ( CHOP ) as one of the HRG inducible genes . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| The human genes for C / EBP homology protein ( chop ) and asparagine synthetase ( AS ) are model systems to investigate transcription induced by nutrient limitation and endoplasmic reticulum ( ER ) stress . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Here we show that inhibitory / regulatory C / EBP family proteins , Ig / EBP ( C / EBPgamma ) and CHOP ( C / EBPzeta ) , but not positively functioning NF IL 6 ( C / EBPbeta ) , are constitutively multiubiquitinated and subsequently degraded by the proteasome . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| PTP epsilon also inhibits transcriptional activation mediated by c Jun and C / EBP binding protein ( CHOP ) but not that mediated by the unrelated NFkB , attesting that it is broadly active within the MAPK family but otherwise specific . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Using several mutants of the CHOP promoter fragments , we found that two regions , CCAAT / enhancer binding protein ( C / EBP ) site at 313 and ER stress element ( ERSE ) at 93 , are involved in activation of the CHOP gene by 15 d PGJ ( 2 ) . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| The gene for the proapoptotic transcription factor CCAAT / enhancer binding protein ( C / EBP ) homologous protein / growth arrest and DNA damage inducible protein 153 ( CHOP / GADD153 ) is induced by various cellular stresses . ^^^ By mutational analysis of the conserved region in the promoter of the CHOP gene , we provide evidence that the C / EBP ATF composite site , but not the ER stress responsive element or the activator protein 1 site , is required for the increased expression of CHOP during PC depletion . ^^^ Taken together , our results suggest that increased expression of CHOP during PC depletion depends on a C / EBP ATF element in its promoter and might be mediated by binding of ATF 2 to this element . . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Under these conditions , we found that mRNA and protein for CHOP / GADD153 , a C / EBP family transcription factor that is involved in ER stress induced apoptosis , were induced . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| C / EBPepsilon was found to associate with other C / EBP family members , including C / EBPepsilon and CHOP as well as other proteins that are known to contain a leucine zipper protein interaction motif including CREB 2 , LDOC 1 , E6TP1 , and AF 17 . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| We have previously shown , by microarray analysis of primary beta cells , that IL 1beta + IFN gamma decrease expression of the mRNA encoding for the sarcoendoplasmic reticulum pump Ca ( 2+ ) ATPase 2b ( SERCA2b ) while inducing expression of the endoplasmic reticulum stress related and proapoptotic gene CHOP ( C / EBP [ CCAAT / enhancer binding protein ] homologous protein ) . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Notably , stress induced transcription factors such as ATF 3 , ATF 4 , CHOP , and C / EBP beta were robustly up regulated , yet exhibited unique kinetic patterns . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Our study revealed that the expression of the CEBP homologous protein ( CHOP ) coding gene is promptly an intensely upregulated following astrocyte oxygen and glucose deprivation . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Subtype selective interaction with the transcription factor CCAAT / enhancer binding protein ( C / EBP ) homologous protein ( CHOP ) regulates cell surface expression of GABA ( B ) receptors . ^^^ Here , we showed that the transcription factor CCAAT / enhancer binding protein ( C / EBP ) homologous protein ( CHOP ) directly interacts with GABA ( B ) receptors in a subtype selective manner to regulate cell surface expression of GABA ( B1a ) / GABA ( B 2 ) receptors upon co expression in HEK 293 cells . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Overexpression of the C / EBP homologous transcription factor CHOP impaired transcriptional activation of the asparagine synthetase promoter following amino acid deprivation or over expression of ATF 5 or CREB2 / ATF4 . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Celecoxib , an NSAID , upregulated ER chaperones ( GRP 78 and its cochaperones ERdj 3 and ERdj 4 ) but also C / EBP homologous transcription factor ( CHOP ) , a transcription factor involved in apoptosis . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| CHOP is a C / EBP family transcription factor involved in endoplasmic reticulum ( ER ) stress mediated apoptosis . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| FAA treatment also causes a subsequent induction of the proapoptotic CHOP ( CEBP homologous protein ) transcription factor as well as a late activation of caspase 12 . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Superoxide anions and H ( 2 ) O ( 2 ) increased mRNA and protein expression of GAS 5 ( growth arrest specific protein 5 ) and CHOP ( C / EBP homology protein ) . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| This event requires cooperativity from the C / EBP family of transcription factors including C / EBPbeta and C / EBPbeta homologous protein ( CHOP ) . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| INS 1 cells exposed to palmitate for 16 24 h under serum free conditions showed marked apoptosis and increased protein levels of phosphorylated eukaryotic translation initiation factor 2alpha ( eIF2alpha ) , activating transcription factor 4 ( ATF 4 ) , 10 box binding protein 1 ( XBP 1 ) , and C / EBP homologous transcription factor ( CHOP ) compared with control cells . ^^^ |
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| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Myxoid liposarcoma FUS DDIT 3 fusion oncogene induces C / EBP beta mediated interleukin 6 expression . ^^^ We here report that the NFkappaB and C / EBP beta controlled gene IL 6 is upregulated in DDIT 3 and FUS DDIT 3 expressing fibrosarcoma cell lines and in myxoid liposarcoma cell lines . ^^^ Knockdown experiments using siRNA against CEBPB transcripts showed that the effect of FUS DDIT 3 on IL 6 expression is C / EBP beta dependent . ^^^ We demonstrate for the first time that DDIT 3 and FUS DDIT 3 show opposite transcriptional regulation of IL 8 and suggest that FUS DDIT 3 may affect the synergistic activation of promoters regulated by C / EBP beta and NFkappaB . . ^^^ |
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| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Of the three known C / EBP family inhibitors , Ig / EBP , LIP and CHOP 10 , only Ig / EBP is ubiquitously expressed . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Expression of C / EBP alpha increased basal PRL gene expression almost 6 fold , whereas expression of Chop 10 that can act as an inhibitor of C / EBP alpha reduced the basal activity of the PRL promoter 60 75 % . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Finally , the overall importance of C / EBP proteins for lytic gene expression was demonstrated using CHOP 10 to antagonize C / EBP DNA binding activity . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| After an 8 day treatment , a set of 39 genes was found to be differentially expressed in cells treated with antimycin A , among them CCAAT / enhancer binding protein alpha ( C / EBPalpha ) , C / EBP homologous protein 10 ( CHOP 10 ) , mitochondrial glycerol 3 phosphate dehydrogenase ( GPDmit ) , and stearoyl CoA desaturase 1 ( SCD 1 ) . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Calcium ionophore A 23187 induces expression of the growth arrest and DNA damage inducible CCAAT / enhancer binding protein ( C / EBP ) related gene , gadd 153 . ^^^ Ca2+ increases transcriptional activity and mRNA stability . gadd 153 is a CCAAT / enhancer binding protein ( C / EBP ) related gene whose expression is induced in response to growth arrest and DNA damage . ^^^ |
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| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Induction of GADD 153 , a CCAAT / enhancer binding protein ( C / EBP ) related gene , during the acute phase response in rats . ^^^ GADD 153 , a unique member of the C / EBP family , has been proposed to act as a dominant negative inhibitor of other C / EBPs , but little is known about its expression in liver or its role in the processes described above . ^^^ We have examined its expression during the acute phase response ( APR ) and have shown that like C / EBP beta and C / EBP delta , GADD 153 mRNA is markedly induced in livers of rats treated with lipopolysaccharide to initiate the APR . ^^^ Footprint analysis of the GADD 153 promoter showed binding of proteins in liver extracts of both untreated and lipopolysaccharide injected rats to a putative C / EBP regulatory site . ^^^ Support for the functional role of this C / EBP binding site and its interaction with C / EBPs in regulating GADD 153 expression was obtained with cultured HepG 2 hepatoma cells in which overexpression of C / EBP beta was found to transactivate expression of a plasmid containing the GADD 153 promoter linked to a reporter gene . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Induction of GADD 153 , a CCAAT / enhancer binding protein ( C / EBP ) related gene , during the acute phase response in rats . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Class 3 genes [ gadd 153 , beta actin , ubiquitin ( UbC ) , phosphoglycerate kinase 1 , C / EBP alpha , C / EBP beta ] exhibit increased expression in response to amino acid limitation . ^^^ The strong induction by amino acid limitation of the C / EBP inhibitor gadd 153 is of interest in this regard , as increased levels of gadd 153 could interfere with C / EBP , which is required for high expression of most liver specific genes . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Regulation of the C / EBP related gene gadd 153 by glucose deprivation . gadd 153 encodes a CCAAT / enhancer binding protein ( C / EBP ) related protein that lacks a functional DNA binding domain . ^^^ Because C / EBP alpha appears to play an important role in regulating genes involved in adipogenesis and energy metabolism , we examined gadd 153 expression during the differentiation of 3T3 L 1 preadipocytes and as a function of glucose utilization in differentiated adipocytes . ^^^ Using a standard differentiation protocol that consisted of hormonal stimulation for 2 days followed by medium changes every 2 days thereafter , we observed that both C / EBP alpha and gadd 153 mRNAs were elevated . ^^^ However , C / EBP alpha induction occurred on day 3 , while gadd 153 expression was not seen until day 4 , when the cells were fully differentiated . ^^^ Frequent addition of fresh medium to the cells during the differentiation process , as well as supplementation of medium with glucose , reduced gadd 153 expression without preventing C / EBP alpha expression or interfering with cellular differentiation . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| GADD 153 , a ubiquitously expressed member of the CCAAT / enhancer binding protein ( C / EBP ) family is induced by a wide variety of growth arresting and DNA damaging agents . ^^^ In this report we have demonstrated that treatment of rat pheochromocytoma PC 12 cells with sodium arsenite leads to enhanced expression of C / EBP beta and GADD 153 ( growth arrest and DNA damage inducible gene 153 ) but not other C / EBPs . ^^^ Coimmunoprecipitation experiments provided evidence for the formation of endogenous GADD 153 C / EBP beta complexes in arsenite treated cells . ^^^ Previous studies in our laboratory demonstrated that the GADD 153 promoter contains a C / EBP binding site through which other C / EBPs interact to transactivate GADD 153 expression in liver hepatoma cells . ^^^ Here , we demonstrate that extracts prepared from arsenite treated PC 12 cells likewise show increased amounts of factors capable of binding to the GADD 153 C / EBP site and that these complexes are comprised at least in part of C / EBP beta . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Induction of hemopexin , alpha 1 acid glycoprotein , alpha 2 macroglobulin , GADD 153 , C / EBP beta , and C / EBP delta mRNAs by LPS were all abolished in dexamethasone treated rats . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Transfection experiments using GADD 153 to create C / EBP null conditions confirm that C / EBP factors are absolutely required for promoter activity and TPA responsiveness . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| The gadd 153 gene belongs to the CCAAT / enhancer binding protein ( C / EBP ) family of transcription factors . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Two of the most prominently induced genes encoded the growth arrest and DNA damage inducible protein Gadd 153 and the activating transcription factor ATF 3 , both transcription factors of the CCAAT / enhancer binding protein ( C / EBP ) family . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| However , we also demonstrate increased expression of a stress responsive C / EBP homologue , GADD 153 ( growth arrest and DNA damage gene 153 ) , during monolayer culture , which may promote dedifferentiation . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Growth arrest and DNA damage gene 153 ( GADD 153 ) is a member of the CCAAT / enhancer binding protein ( C / EBP ) family of transcription factors and has been associated with apoptosis . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| By further promoter analyses , a novel DCA response element , which is located downstream of the AP 1 binding site in the human GADD 153 promoter , was determined and identified as C / EBP regulatory element . ^^^ These results suggest that GADD 153 expression is critical for DCA induced apoptosis and that multiple signaling pathways that include AP 1 and C / EBP transcription factors are involved in DCA induced GADD 153 expression . . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| These responses are eliminated by the dominant negative C / EBP isoform , GADD 153 , and are also inhibited by dominant negative forms of Ras , MEKK 1 , MEK 3 , and p 38 . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| GADD 153 significantly antagonized the C / EBP ( C / EBP alpha , beta , and delta ) mediated transcriptional activation of the Tf gene . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Growth arrest and DNA damage inducible gene 153 ( GADD 153 ) is a CCAAT / enhancer binding protein ( C / EBP ) related gene and is induced in response to various stimuli including DNA damaging agents , UV irradiation , and serum starvation . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| Induction of C / EBP beta and GADD 153 expression by dopamine in human neuroblastoma cells . ^^^ In addition , overexpression of C / EBP beta caused a concomitant increase in the expression of alpha synuclein but not of GADD 153 . ^^^ Inhibition of JNK by SP 600125 reduced increases in C / EBP beta and alpha synuclein expression , whereas inhibition of both JNK and p38MAPK ( with SB 203580 ) blocked the increase in GADD 153 expression . ^^^ We conclude that dopamine , through a mechanism driven by stress activated MAPKs , triggers C / EBP beta and GADD 153 expression in a dose dependent way . ^^^ |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35638 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|