| To verify the cell lineage after LIPUS stimulation , mRNA expression of cellular phenotype specific markers characterizing osteoblasts ( Runx 2 , Msx 2 , Dlx 5 , AJ 18 ) , chondroblasts ( Sox 9 ) , myoblasts ( MyoD ) , and adipocytes ( C / EBP , PPARgamma ) was studied using real time polymerase chain reaction analysis . ^^^ The mRNA expression of Runx 2 , Msx 2 , Dlx 5 , AJ 18 , and Sox 9 was increased markedly by the LIPUS stimulation , whereas the expression of MyoD , C / EBP , and PPARgamma was drastically decreased . ^^^ |