| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.69225142 |
| The transcription factor HNF 1 acts with C / EBP alpha to synergistically activate the human albumin promoter through a novel domain . 0.69225142^^^ Our studies delineate a domain in HNF 1 important for synergistic activation with C / EBP alpha . . 0.55587491^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.51209279 |
| Transcriptional synergism between HNF 1 and C / EBP was observed in these transactivation experiments . 0.51209279^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.90359841 |
| These results demonstrate functional interactions between HNF 1 , C / EBP , and CDX family factors and suggest that these interactions may contribute to differential transcriptional regulation in the small intestinal crypt and villus compartments . . 0.90359841^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| We examined the role of upstream DNA and liver specific transcription factors in regulation of this promoter by use of transient transfection of heterologous chloramphenicol acetyltransferase gene constructs and vectors containing cDNAs encoding the liver enriched transcription factors HNF 1 alpha , C / EBP , and DBP . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| They express mRNAs for liver enriched transcription factors including HNF 1 alpha , HNF 3 beta and gamma , HNF 4 , and members of the CCAAT enhancer binding protein ( C / EBP ) family . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| However , the expression of the transcription factors C / EBP , HNF 1 alpha and HNF 4 decreased after 14 days of TCDD treatment . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Only HNF 1 alpha , and not HNF 1 beta , HNF 4 , C / EBP alpha , C / EBP beta , or DBP , was able to activate the CYP2E1 promoter . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Aldolase B gene expression appeared restricted to the proximal tubule , the site where HNF 1 alpha , HNF 1 beta , C / EBP alpha , and DBP transcripts were also abundant . ^^^ Transactivation experiments in PKSV PCT cultured cells showed that HNF 1 alpha , C / EBP alpha , and DBP behave as transactivators of the 190 bp aldolase B gene promoter , and that HNF 1 beta had a low transactivating efficiency . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Unlike the fetal liver , however , Hepa cells failed to express the early expressed factors hepatocyte nuclear factor 1 alpha ( HNF 1 alpha ) and HNF 4 and the late expressed factor C / EBP alpha , thereby providing a suitable system for examining possible cooperation between these factors in the transcriptional regulation of the PEPCK gene . ^^^ Transient transfection assays of a chimeric PEPCK chloramphenicol acetyltransferase construct showed a residual PEPCK promoter activity in the Hepa cell line , which was slightly stimulated by cotransfection with a single transcription factor from either the C / EBP family or HNF 1 alpha but not at all affected by cotransfection of HNF 4 . ^^^ In contrast , cotransfection of the PEPCK construct with members from the C / EBP family plus HNF 1 alpha resulted in a synergistic stimulation of the PEPCK promoter activity . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| To better understand the regulation of this process we have studied the expression of liver enriched transcriptional factors ( HNF 1 alpha and HNF 1 beta , HNF 3 alpha , HNF 3 beta , and HNF 3 gamma , HNF 4 , C / EBP , C / EBP beta , and DBP ) in an experimental model of oval cell proliferation and differentiation and compared the expression of these factors to that observed during late stages of hepatic ontogenesis . ^^^ The steady state mRNA levels of four ( HNF 1 alpha , HNF 3 alpha , HNF 4 , and C / EBP beta ) `` liver enriched ' ' transcriptional factors gradually decrease during the late period of embryonic liver development while three factors ( HNF 1 beta , HNF 3 beta , and DBP ) increase . ^^^ However , during early stages of oval cell proliferation both small and large bile ducts start to express HNF 1 alpha and HNF 1 beta , HNF 3 gamma , C / EBP , and DBP but not HNF 4 . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| The minimal burn injury model leads to the molecular induction of the acute phase response and induces significant and rapid changes in the liver specific transcription factors C / EBP alpha , HNF 1 alpha , and HNF 4 . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| DNase 1 footprinting and gel mobility shift assays demonstrated that the DNA binding activities specific to the albumin enhancer were not decreased in the ras transformed cells . ras also did not diminish the expression of HNF 1 alpha , C / EBP alpha , HNF 3 alpha , HNF 3 beta , or HNF 3 gamma but did significantly increase AP 1 binding activity . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Moreover , the transactivating ability of endogenous HNF 1 alpha and C / EBP alpha was also evaluated by measuring the activity of transfected synthetic promoters consisting of DNA element homopolymers upstream of a TATA box . ^^^ We investigated the influence of HNF 1 alpha and C / EBP alpha on the activity of transfected liver specific promoters . ^^^ In contrast , the activity of the albumin promoter in the different lines was clearly dependent on the C / EBP alpha content , which seems , therefore , to be an essential factor modulating the expression of this gene in HNF 1 alpha containing cells . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Activity was markedly increased by cotransfection with a vector expressing C / EBP beta but was unaffected by vectors producing other liver enriched transcription factors ( C / EBP alpha , HNF 1 alpha , and DBP ) . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Recent studies have found that several different liver enriched transcription factors including HNF 1 alpha , HNF 3 , HNF 4 , and C / EBP beta , and the more ubiquitously expressed factors Sp 1 , GABP alpha / beta , and NF2d9 are responsible for governing the transcription of P 450 genes . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Transcription factors C / EBP alpha and HNF 1alpha are associated with decreased expression of liver specific genes in sepsis . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Our results showed that comprehensive expressions of HNF 3beta , HNF 4 alpha , HNF 1 alpha , and C / EBP alpha were specific to HCCs with well differentiated function and morphology . ^^^ These findings suggested that HNF 4 alpha and HNF 1 alpha are useful markers to assess the degree of HCC differentiation , which we suggest could be evaluated objectively by the quantitative analysis of HNFs and C / EBP alpha in HCCs . . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| However , sequence analysis of the common carp IGF 1 promoter region identified several consensus liver enriched transcription factor binding sites , including HNF 1alpha , HNF 3beta , C / EBP , and one STAT5 . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Of interest , the expression of cyclin genes ( A 2 , B , D 1 , and E ) , hepatocyte nuclear factor genes ( HNF 1alpha , beta , 3beta , and 4alpha ) , and oncogenic markers ( P 53 , c myc , beta catenin , N ras , and gankyrin ) were up regulated , while the expression of C / EBP alpha was down regulated in a zfMcl 1a mediated anti apoptotic process of the liver . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Liver specific transcription factors of the HNF 3 , C / EBP and LFB 1 families interact with the A activator binding site . ^^^ The A activator binding site ( AABS ) , present in the Xenopus A 2 vitellogenin gene and several mammalian liver specifically expressed genes , interacts with different liver specific transcription factors including LFB 1 and C / EBP isobinders . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Chromosomal localization in man and rat of the genes encoding the liver enriched transcription factors C / EBP , DBP , and HNF1 / LFB 1 ( CEBP , DBP , and transcription factor 1 , TCF 1 , respectively ) and of the hepatocyte growth factor / scatter factor gene ( HGF ) . ^^^ By means of somatic cell hybrids segregating either human or rat chromosomes , we determined the chromosome localization of three genes encoding transcription factors expressed in hepatocytes , namely , C / EBP ( CCAAT / enhancer binding protein ) , DBP ( D site of albumin promoter binding protein ) , and HNF1 / LFB 1 ( designated transcription factor 1 , gene symbol : TCF 1 ) , and of the hepatocyte growth factor gene , which is identical to the mitogenic and chemotactic factor designated scatter factor ( gene symbol : HGF ) . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| The present study analyzed the expression of the following 13 TFs : AML 1 , CEBPA , E2A , ETS 1 , GATA 1 , GATA 2 , GATA 3 , IKAROS , IRF 1 , PAX 5 , PU 1 , TBET and TCF 1 in 7 malignant NK cell lines together with 5 malignant NKT cell lines , 5 T cell acute lymphoblastic leukemia ( ALL ) cell lines including 3 gamma / delta T cell receptor ( TCR ) type and 2 alpha / beta TCR type , and 3 B cell precursor ( BCP ) leukemia cell lines . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| The cells selectively increased phosphorylation of p 38 MAPK , upstream dual activator MAPK kinase 3 / 6 , and downstream transcription factors activating transcription factor 2 , ETS domain transcription factor 1 , and C / EBP homologous protein in a time dependent manner . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Putative response elements for the transcription factors hepatocyte nuclear factor 4 ( HNF 4 ) alpha , CCAAT box / enhancer binding protein ( C / EBP ) alpha , C / EBPbeta , and octamer transcription factor 1 ( Oct 1 ) were identified in this region , and electrophoretic mobility shift assays showed that these transcription factors bind to the predicted elements . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| We hypothesize that the transcription factors C / EBP and DBP are the principal tissue specific regulators in liver , and that HNF 1 and perhaps C / EBP are important for kidney specific PEPCK expression . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Multiple cis acting sequences to which nuclear proteins bind have been mapped , and transcription factors that can bind to these sequences have been identified ; these include C / EBP alpha , Sp 1 , USF , HNF 1 , CTF / NF1 , the glucocorticoid receptor , and RAR alpha . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| In c14CoS mice , transcription rates of transcription factor genes HNF 1 , C / EBP and HNF 4 were reduced , albeit to different extents . ^^^ Levels of HNF 1 and HNF 4 mRNAs showed much greater depression than that of C / EBP in mutant liver . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Only HNF 1 , and to a lesser extent C / EBP , show differences depending upon the differentiation state of the cells . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| The binding site for a nuclear factor designated protein 1 is located between the HNF 1 and C / EBP binding sites . ^^^ Mutations that abolish the binding of either HNF 1 , protein 2 , or the C / EBP related proteins severely reduce enhancer activity . ^^^ However , deletion experiments demonstrated that neither the HNF 1 binding site alone , nor the combination of binding sites for HNF 1 , protein 1 , and C / EBP , nor the C / EBP binding site plus the protein 2 binding site is sufficient to enhance transcription from a strong apolipoprotein B promoter . ^^^ Rather , HNF 1 and C / EBP act synergistically with protein 2 to enhance transcription of the apolipoprotein B gene . . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| For all three promoters , the activation by LAP or DBP was higher than that seen by HNF 1 or C / EBP alpha , and a significant synergism between C / EBP alpha and LAP was noticed for the ADH 2 and ADH 3 promoters when both factors were simultaneously cotransfected . ^^^ These studies suggest that HNF 1 and C / EBP alpha help establish ADH gene family transcription in fetal liver and that LAP and DBP help maintain high level ADH gene family transcription in postnatal liver . . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| The trans acting factors of the mouse alpha fetoprotein proximal promoter ( 202 base pairs ) are aligned as follows : regions Ia ( HNF 1 ) , Ib ( C / EBP ) , 2 ( NF 1 or C / EBP ) , 2 ' ( NF 1 or HNF 1 ) , 3 ( NP 3 ) , 4 ( NP 4 ) , Va ( NP Va ) , and Vb ( C / EBP ) . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Binding to this region was competed by oligonucleotides corresponding to the albumin D site , but not by oligonucleotides corresponding to binding sites for the hepatocyte transcription factors HNF 1 , HNF 3 , HNF 4 , and C / EBP . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Interplay of an original combination of factors : C / EBP , NFY , HNF 3 , and HNF 1 in the rat aldolase B gene promoter . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| The different tissue transcription patterns of genes for HNF 1 , C / EBP , HNF 3 , and HNF 4 , protein factors that govern liver specific transcription . ^^^ The transcription factors that act in hepatocyte specific gene expression include proteins that are present mainly in liver cells ( HNF 1 / LFB1 , C / EBP , HNF 3 , HNF 4 ) ( HNF , hepatocyte nuclear factor ; C / EBP , rat enhancer binding protein ) and proteins that are widely distributed ( AP 1 , NF 1 , NF Y / ACF ) . ^^^ These experiments identify C / EBP , HNF 3 , and HNF 4 , and perhaps also HNF 1 , as targets for the study of cascades of transcriptionally controlled transcription factors in differentiated cells . . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| This DNA fragment could form DNA protein complexes with heat stable nuclear proteins , and the complexes formed could be specifically competed for by excess oligomers corresponding to the C / EBP or DBP binding sites but not by binding sites for three other liver specific factors , HNF 1 , HNF 3 , and HNF 4 . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Contrary to HNF 1 , the mRNAs coding for two other nuclear factors involved in albumin transcription , C / EBP and NF 1 , do not follow the distribution of albumin transcripts in these cell lines . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Four hepatocyte nuclear factors ( HNF 1 , 3 , and 4 and C / EBP ) that are present in liver but not in most other adult tissues bind DNA sites in the TTR gene that are sufficient to direct transgenic expression . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| The promoter of the rat alpha fetoprotein ( AFP ) gene , which makes the expression of the developmentally regulated AFP gene specific to the liver , is a putative target for transcription factors of the CAAT / enhancer binding protein ( C / EBP ) , hepatocyte nuclear factor 1 ( HNF 1 ) and nuclear factor 1 ( NF 1 ) families . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Transcription factors of the CCAAT / enhance binding protein ( C / EBP ) , hepatocyte nuclear factor 1 ( HNF 1 ) , and nuclear factor 1 ( NF 1 ) families can bind in vitro to the promoter of the rat AFP gene , which makes the expression of the AFP gene specific to the liver . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| The immunoprecipitate includes RNA polymerase 2 , TFIID , TFIIB , TFIIH , TFIIF , and TFIIE , but is devoid of transcriptional activator proteins , such as HNF 1 , HNF 4 , and C / EBP alpha . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Neither were the concentrations of various liver specific transcription factors ( HNF 3 , HNF 1 , HNF 4 , and C / EBP alpha ) affected . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| The promoter proximal sequences of the albumin gene interact with a number of tissue specific transcription factors including HNF 1 and C / EBP . ^^^ We determined the binding activities of liver nuclear extracts to the HNF 1 and C / EBP binding sites by gel mobility shift assay and found that the activities of the extract prepared from liver of vitamin B 6 deficient rats were greater than those of controls . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Expression of the liver enriched transcription factors C / EBP alpha , C / EBP beta , HNF 1 , and HNF 4 in preneoplastic nodules and hepatocellular carcinoma in rat liver . ^^^ However , no detectable changes were observed in preneoplastic nodules in the transcription or in steady state mRNA levels of C / EBP beta , HNF 1 , and HNF 4 . ^^^ A slight decrease in C / EBP beta protein and a more pronounced attenuation of HNF 1 and HNF 4 levels was observed in nodules , being 67 % , 37 % , and 46 % of the levels in the corresponding surrounding livers , respectively . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Analysis of transacting factors by gel retardation assay revealed that HNF 1 , COUP TF and SP 1 were detected at almost the same level in the hepatic nuclear fraction of jvs mice as in normal littermates , and C / EBP and CREB were a little higher in jvs mice , suggesting that these factors are probably not targets of jvs mutation causing abnormal gene expression in the liver . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Several cis acting elements in the 5 ' flanking region that potentially regulate transcription were identified : two glucocorticoid responsive elements , two CCAAT enhancer binding protein C ( C / EBP ) consensus elements that are required for the interleukin 1 responsiveness of some acute phase reactant genes , an interleukin 6 responsive element , two hepatocyte nuclear factor 1 ( HNF 1 ) elements and a single heat shock element . ^^^ Transfection of the hepatoma cell line Hep 3B . 2 with a pCAT expression vector containing the 5 ' flanking sequence from 1083 to 3 bp from the transcriptional start site , and truncations of this sequence , localized elements that control the tissue specific expression of mouse C reactive protein to the two HNF 1 elements and a C / EBP , interleukin 1 responsive element located between 220 and 153 , and 90 and 50 bp from the transcriptional start site . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| HNF 1 and C / EBP related proteins are transcription factors important for the activation of albumin gene expression . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Among the sites that were tentatively identified were one C / EBP , one HNF 1 , and two HNF 3 binding sites . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| The loss of CYP1A2 and 2B1 / 2 mRNAs parallels the loss of the mRNAs encoding the liver specific transcription factors C / EBP alpha and HNF 1 . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Three nuclear protein binding sites that showed rapid CCl 4 induced kinetic changes were homologous to the consensus sequence for the binding of the transcription factor families MEF 2 , HNF 1 , and C / EBP . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Differential patterns of expression of three C / EBP isoforms , HNF 1 , and HNF 4 after partial hepatectomy in rats . ^^^ A set of transcription factors termed hepatocyte nuclear factors ( HNF 1 , 3 , 4 ) and CCAAT / enhancer binding protein ( C / EBP ) isoforms are known to regulate several genes predominantly expressed in the liver . ^^^ To assess whether these factors might be involved in the hepatocyte proliferation program , we have studied the expression of the three C / EBP isoforms C / EBP alpha , C / EBP beta , and C / EBP delta and the two hepatocyte enriched transcription factors , HNF 1 and HNF 4 , in rat liver at various time points after partial hepatectomy and sham operations using transcriptional `` run on ' ' assays and Northern blot and Western blot experiments . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Despite the importance of HNF 1 , HNF 4 , NF 1 , and C / EBP alpha in the regulation of numerous other target genes , deletion of the HNF 1 , NF 1 , and HNF 4 and several C / EBP binding sites did not drastically affect the hepatic transcription of the apoA 2 gene . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| DNase 1 footprinting analysis and mobility shift assays demonstrate that this enhancer of 192 bp contains at least three binding sites for transcription factors : one for hepatocyte adipocyte C / EBP , a second for the liver specific transactivator hepatocyte nuclear factor 1 HNF 1 , and a third for a factor , called F 3 , which binds to a DNA sequence bearing some resemblance to that for the ubiquitous factor EF C . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| These include the hepatocyte nuclear factors 1 , 3 , 4 ( HNF 1 , 3 , 4 ) , some members of the CAAAT / enhancer binding protein ( C / EBP ) family , and D site binding protein ( DBP ) . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Competition between transcription factors HNF 1 and HNF 3 , and alternative cell specific activation by DBP and C / EBP contribute to the regulation of the liver specific aldolase B promoter . ^^^ From 5 ' to 3 ' , we found : a negative DE element , an activating C / EBP DBP binding site , a CCAAT box binding NFY that seems to play a negative role , and an activating element consisting of two overlapping binding sites for HNF 1 and HNF 3 . ^^^ We found that DBP and HNF 1 are strong transactivators of the aldolase B promoter while C / EBP and vHNF 1 are only weak activators and HNF 3 alone does not modify such activity . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| This enhancer contains multiple protein binding sites , including sites for ubiquitous factors ( NF 1 and AP 1 ) , the glucocorticoid receptor , and the hepatocyte enriched transcription factors hepatocyte nuclear factor 1 ( HNF 1 ) and C / EBP . ^^^ Efficient transcription from the highly inducible promoter shows absolute dependence upon the enhancer at kb 3 . 5 , which in turn requires HNF 1 and C / EBP as well as hormones . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| In contrast , nuclear expression of other factors such as HNF 1 or C / EBP alpha , which also have been shown to transcriptionally control albumin expression , is either unchanged or slightly decreased . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Two other footprinted sites correspond to binding sites for the liver transcription factors C / EBP and albumin D element binding protein and a third to an HNF 1 binding site . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Since the P 2 site is not involved in the expression of the PEPCK gene in the liver , we propose that its critical role in the kidney stems from a combination of abundance of HNF 1 together with low concentrations of members of the C / EBP family in this tissue . . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Northern blot analysis revealed that there were slightly more transcripts of C / EBP , HNF 1 beta ( n . s . ) and HNF 4 ( p < 0 . 05 ) , but fewer of HNF 3 gamma ( n . s . ) , in perivenous than in periportal lysates . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| The 5 ' flanking region contains potential binding sites for TATA binding protein , Sp 1 , nuclear factor 1 ( NF 1 ) , CAAT box binding protein ( C / EBP ) , hepatocyte nuclear factors 1 and 5 ( HNF 1 , HNF 5 ) and activator proteins 1 and 2 ( AP 1 , AP 2 ) . . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Several high probability transcription factor recognition sequences , including proteins that are enriched in , or specific to , the liver , such as C / EBP alpha , C / EBP beta , HNF 1 , and HNF 3 beta , have been located in the 5 ' region of the gene . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| DNase 1 footprinting and electrophoretic mobility shift analyses revealed two hepatocyte nuclear factor 1 ( HNF 1 ) , three CCAAT / enhancer binding protein ( C / EBP ) , and one consensus palindromic thyroid hormone response elements within the first 215 base pairs ( bp ) of the promoter sequence of rat Std . ^^^ However , overexpression of HNF 1 and C / EBP resulted in synergistic activation of the Std promoter in this cell type , indicating essential roles of these two trans regulators in liver selective expression of the rat Std gene . ^^^ Thus , these studies on rat Std promoter function indicate that ( 1 ) HNF 1 and C / EBP are responsible for liver specificity of the rat Std gene ; ( 2 ) androgenic repression of the gene requires the presence of all of the OCT 1 and C / EBP elements between positions 231 and 292 ; and ( 3 ) AR may exert its negative regulatory effect indirectly through transcriptional interference of OCT 1 and C / EBP rather than through a direct DNA AR interaction . . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| The 3 . 0 kb ALDH 3 XRE region contains multiple overlapping transcription factor binding sequences , including consensus sites for AhR , ARNT , HNF 1 , HNF 4 , and C / ebp . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Analysis of the expression of relevant hepatic genes by reverse Northern and semiquantitative reverse transcriptase polymerase chain reaction ( RT PCR ) revealed that culturing hepatocytes in collagen gels results in a sustained higher expression of key liver transcription factor genes DBP , C / EBP alpha and beta , and HNF 1 and 4 , as well as specific liver enzyme genes ( phosphoenol pyryvate carboxykinase , and carbamoylphosphate synthetase 1 ) . . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| In addition , their liver remnants were HGF positive and showed increased DNA binding of transcription factors engaged in the modulation of hepatocyte proliferation ( e . g . , STAT 3 ) and liver specific gene expression ( e . g . , HNF 1 , HNF 4 , C / EBP ) . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| The peak [ 3H ] thymidine uptake induced by 20 ng / ml of either HGF or EGF was seen after 72 h ; however , DNA binding of C / EBP and HNF 1 decreased already after 6 h ( electrophoretic mobility shift assay ) . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| By analysing DNA sequences implicated in liver specific transcription , it has been possible to identify members of the nuclear proteins , such as the liver enriched transactivating factors , hepatic nuclear factor 1 ( HNF 1 ) , HNF 3 , HNF 4 , HNF 6 , CCAAT / enhancer binding protein ( C / EBP ) , and D binding protein ( DBP ) , which are key elements in the liver specific transcriptional regulation of genes . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| The intact 2000 bp PEPCK promoter could be markedly activated by co transfecting the transcription factor HNF 1 together with C / EBP . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| In the work presented here , we examined whether the coordinate repression of these genes is caused by decreased activity of one or more of the liver enriched transcription factors , hepatocyte nuclear factor 1 ( HNF 1 ) , HNF 3 , HNF 4 or C / EBP . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Analysis in somatic hybrid cells created from NIH 3T3 fibroblasts and Hepa 1 6 mouse hepatocytes with expression of albumin and selected hepatic transcription factors showed that HBV sequences were expressed weakly but without increased expression following transfection of HNF 1 , HNF 3 , and C / EBP plasmids . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| The rat promoter contained no classical TATA or CAAT boxes but putative binding sites for the transcription factors C / EBP , GATA 1 , HNF 1 , HNF 3beta , SP 1 , and USF . ^^^ |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| It contains HNF 1 , HNF 4 , HNF 3 and C / EBP binding sites as shown by DNase 1 footprinting and electrophoretic mobility shift assays using nuclear extracts and / or recombinant HNF1alpha and HNF4alpha1 . ^^^ Mutation analyses showed that the HNF 1 site is essential for HNF1alpha transactivation and is required for full basal enhancer activity , as is the C / EBP site . ^^^ |
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| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| While expression of the rodent gene relies upon two modules in the HSIII enhancer , one activated by HNF 1 and C / EBP and the other required for the hormone response , the human equivalent has conserved only the liver specific transcription factor binding module . ^^^ |
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| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| We have now investigated other TFs , such as activator protein 1 ( AP 1 ) and hypoxia inducible factor 1 ( HIF 1 ) that we had shown to be activated in rat liver during heat shock , ischemia or post ischemic reperfusion , and signal transducer and activator of transcription ( STAT ) , CCAAT / enhancer binding protein ( C / EBP ) and hepatocyte nuclear factor 1 ( HNF 1 ) , which may be involved in the response of the liver to injury . ^^^ |
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| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| An enhancer element has recently been characterized 6 kb 5 ' of the HNF4alpha P 1 promoter containing binding sites for the transcription factors HNF 1 , HNF 4 , HNF 3 , and C / EBP , which are overlapped by glucocorticoid consensus sites . ^^^ |
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| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Reduction in transporter gene expression seems to be mediated by a diminished nuclear binding activity of transactivators such as HNF 1 , C / EBP and PXR by estrogens . . ^^^ |
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| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| The 5 ' flanking region of the IL 8 gene contains potential binding sites for several nuclear factors including activated protein 1 ( AP 1 ) , activated protein 2 ( AP 2 ) , nuclear factor gene binding ( NF kappa B ) , nuclear factor interleukin 6 ( NF IL 6 , also calls CAAT / enhancer binding proteins , C / EBP ) , IFN regulatory factor 1 ( IRF 1 ) , hepatocyte nuclear factor 1 ( HNF 1 ) , and so on . ^^^ |
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| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| The liver specific activity of ENII is regulated by multiple liver enriched transcription factors , including LRH 1 / hB1F , HNF 1 , HNF3b , HNF 4 and C / EBP . ^^^ |
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| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Thus , expression of HNF3gamma , C / EBP alpha , and C / EBP beta may be necessary for hepatocytes to acquire highly differentiated functions in addition to coexpression of certain amounts of transcripts of HNF1alpha , HNF1beta , HNF3alpha , HNF3beta , and HNF 4 as well as suppression of C / EBP delta . . ^^^ |
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| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| When a DNA fragment ( 110 / 86 ) from this region was cloned in front of an SV 40 promoter it showed down regulation by TDCA . ' Super ' electrophoretic mobility shift assays ( EMSA ) indicated that both HNF1alpha and C / EBP bind to the 110 to 86 bp DNA fragment . ^^^ Recombinant rat HNF1alpha and C / EBPalpha competitively bound to this DNA fragment . ' Super ' EMSA showed that TDCA addition to hepatocytes in culture decreased HNF1alpha , but not C / EBP , binding to the 110 / 86 bp DNA fragment . ^^^ The substitution mutation also eliminated ( > 95 % ) HNF1alpha , but not C / EBP , binding to this DNA fragment . ^^^ |
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| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Taken together , these findings suggest that the LPS induced down regulation of Oatp 4 is likely due to reduction in the binding of HNF1alpha , C / EBP , HNF 3 , and RXR : RAR to the Oatp 4 promoter . . ^^^ |
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| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Developmental changes in the expression of the liver enriched transcription factors LF B 1 , C / EBP , DBP and LAP / LIP in relation to the expression of albumin , alpha fetoprotein , carbamoylphosphate synthase and lactase mRNA . ^^^ We investigated by in situ hybridization whether this set of transcription factors , LF B 1 , C / EBP , DBP and LAP / LIP , is expressed coordinately in the liver during embryonic development and to what extent they are also expressed elsewhere . ^^^ |
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| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| Analysis of the 5 ' flanking sequence of HPRT 2 revealed regions with homology to the liver specific regulatory motifs C / EBP , NF IL 6 , LF A 1 and LF B 1 , although the functional significance of these regions remains unknown . ^^^ |
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| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P20823 and P49715 |
Pubmed |
SVM Score :0.0 |
| NA |
|