Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.56501798
Furthermore , C / EBP beta and C / EBP delta readily form heterodimers with one another as well as with C / EBP alpha . 0.56501798^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.63847817
Using a 32P labeled protein probe consisting of the bZip domain of C / EBP beta , we isolated a clone encoding C / EBP related ATF ( C / ATF ) , a bZip protein that heterodimerizes with C / EBP like proteins but belongs to the CREB / ATF family . 0.63847817^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.50802533
With a mammalian two hybrid system , coimmunoprecipitation experiments , and in vitro binding studies , we demonstrated that C / EBP beta and CREB interacted directly ; this interaction involved the C terminus of C / EBP beta but occurred independently of CREB ' s leucine zipper domain . 0.50802533^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.59175881
To determine the contribution of systemic versus cell autonomous functions of C / EBP beta to specific phenotypes , mice with a conditional ' floxed ' C / EBP beta null allele were generated . 0.59175881^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
In addition , C / EBP beta and C / EBP gamma readily heterodimerize with each other as well as with C / EBP alpha . ^^^ The mRNAs coding for C / EBP beta and C / EBP gamma are expressed in a wider variety of rat tissues than C / EBP alpha mRNA , including yolk sac and fetal liver . ^^^ The high levels of C / EBP beta and gamma mRNAs in rat yolk sac and fetal liver , where C / EBP alpha is poorly expressed , suggest that C / EBP beta and / or gamma could be preponderant or early regulators of the AFP gene in these tissues . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Using Western blotting and immunohistochemical staining , we have determined the temporal order of expression for C / EBP alpha , C / EBP beta , and C / EBP delta in differentiating myelomonocytic marrow cells . ^^^ While we have detected C / EBP alpha , C / EBP beta , and C / EBP delta in multiple myeloid leukemia cell lines , and C / EBP alpha in normal myeloid cells and in de novo human myeloid leukemias , we have not detected these C / EBP isoforms in either erythroid or lymphoid cells . ^^^ Finally , we show that C / EBP alpha , C / EBP beta , and C / EBP delta protein and messenger RNA levels correlate in maturing granulocytic cells . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The CCAAT / enhancer binding protein ( C / EBP alpha ) gene ( CEBPA ) maps to human chromosome 19q13 . 1 and the related nuclear factor NF IL 6 ( C / EBP beta ) gene ( CEBPB ) maps to human chromosome 20q13 . 1 . ^^^ The CEBPA gene encoding CCAAT / enhancer binding protein ( C / EBP alpha ) has been mapped to human chromosome 19 and the CEBPB ( formerly TCF 5 ) gene encoding NF IL 6 ( C / EBP beta ) to human chromosome 20 by Southern blot analysis of Chinese hamster 10 human and mouse 10 human somatic cell hybrids . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
For all three promoters , the activation by LAP or DBP was higher than that seen by HNF 1 or C / EBP alpha , and a significant synergism between C / EBP alpha and LAP was noticed for the ADH 2 and ADH 3 promoters when both factors were simultaneously cotransfected . ^^^ A hierarchy of ADH promoter responsiveness to C / EBP alpha and LAP homo and heterodimers is suggested . ^^^ In all three ADH genes , LAP bound to the same four sites previously reported for C / EBP alpha ( i . e . , 160 , 120 , 40 , and 20 bp ) , but DBP bound strongly only to the site located at 40 bp relative to the transcriptional start . ^^^ These studies suggest that HNF 1 and C / EBP alpha help establish ADH gene family transcription in fetal liver and that LAP and DBP help maintain high level ADH gene family transcription in postnatal liver . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Analysis of cDNA and genomic clones shows that the murine Ig / EBP ( C / EBP gamma ) gene encodes a small protein with a predicted molecular weight of 16 . 4 kDa which contains C / EBP family basic and leucine zipper domains but lacks the transcriptional activation domains present in C / EBP ( C / EBP alpha ) and NF IL 6 ( C / EBP beta ) . ^^^ In transfection assays Ig / EBP is neither an activator nor a repressor of transcription ; however , Ig / EBP inhibits the transcriptional ability of NF IL 6 ( C / EBP beta ) and C / EBP ( C / EBP alpha ) , acting as a transdominant negative regulator . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
However , the induction of reporter gene expression and the increase of C / EBP beta mRNA by cAMP were not affected by treatment with tumor necrosis factor alpha , which completely inhibited the accumulation of C / EBP alpha mRNA . ^^^ As in the case of C / EBP alpha , C / EBP beta bound to the CCAAT box of the PI promoter . ^^^ C / EBP beta induction , however , precedes that of C / EBP alpha . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
C / EBP alpha , and C / EBP beta acted as transactivators on the AFP promoter , while LIP , a truncated form of C / EBP beta , was a potent negative regulator of the promoter . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The transcription factors C / EBP alpha and C / EBP beta belong to the leucine zipper C / EBP ( CCAAT / enhancer binding protein ) family of DNA binding proteins . ^^^ C / EBP alpha and C / EBP beta are expressed in the liver and are implicated in the control of transcriptional events following following sepsis . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
C / EBP alpha decreased and C / EBP beta and delta increased their expression between days 20 and 22 of foetal development , respectively . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Transient transfections using expression vectors of C / EBP alpha , C / EBP beta and C / EBP delta have shown that while individually all three isoforms can transactivate the alpha 1 acid glycoprotein chloramphenicol acetyltransferase gene transcription , co expression of C / EBP alpha and C / EBP beta isoforms results in lower levels of reporter gene expression than the levels predicted from their additive transactivation level . ^^^ In vitro DNA binding studies have shown that C / EBP alpha and C / EBP beta isoforms both interact and form complexes with the alpha 1 acid glycoprotein gene C / EBP binding element under normal noninduced conditions during which alpha 1 acid glycoprotein is expressed at a very low level . ^^^ Higher than additive levels of reporter gene expression are observed when combinations of C / EBP delta and C / EBP beta or C / EBP delta and C / EBP alpha are used . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
C / EBP alpha , C / EBP beta and D binding protein ( DBP ) acted as trans activators on the AFP promoter , whereas liver inhibitory protein ( LIP ) , a truncated form of C / EBP beta , was a potent negative regulator of the promoter . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Here we provide evidence that C / EBP delta and C / EBP beta play early catalytic roles in the differentiation pathway , relaying the effects of the hormonal stimulants DEX and MIX in a cascade like fashion , leading to the activation of the gene encoding C / EBP alpha . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Before PH , the expressions of C / EBP alpha , C / EBP beta , and C / EBP gamma were similar in the two treatment groups . ^^^ Dimers containing C / EBP alpha and C / EBP beta accounted for virtually all of the C / EBP DNA binding activity and mRNA for PEPCK , the rate limiting hepatocyte enzyme for gluconeogenesis , was barely detected . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Two members of the C / EBP family of basic region leucine zipper proteins enriched in the liver , C / EBP ( C / EBP alpha ) and CRP 2 ( C / EBP beta ) , were previously shown to transactivate the albumin promoter in a cell type dependent manner . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The levels of mRNA for HNF 4 , C / EBP alpha and C / EBP beta were also higher on EHS gel than on TIC . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
An oligonucleotide corresponding to this domain binds to complexes expressed in rat liver that comprise C / EBP alpha C / EBP beta heterodimers ( alpha beta ) as well as C / EBP beta complexed with itself and / or other unidentified nuclear factors ( beta 1 , beta 2 , and beta 3 ) . ^^^ Epidermal growth factor causes further decay of C / EBP alpha polypeptides and DNA binding activity , and down regulates C / EBP beta DNA binding activity as well . ^^^ Addition of transforming growth factor beta completely antagonizes the effects of epidermal growth factor on C / EBP beta activity , and partially overcomes the effect on C / EBP alpha . ^^^ These results demonstrate that the DNA binding activity of C / EBP alpha and C / EBP beta complexes is regulated in the regenerating liver , and in hepatocyte cultures responding to growth factors that regulate their proliferation . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Gel mobility shift and transient expression analyses showed that C / EBP beta and C / EBP isoforms , C / EBP alpha and C / EBP delta , bind to the PISII element and trans activate rnCGM 3 gene expression . ^^^ Moreover , C / EBP beta is expressed in high levels in the placenta , ovary , liver , lung , heart , and spleen , in contrast to C / EBP alpha , which is expressed primarily in the liver and only low levels in the placenta . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
As determined by immunoblot , the level of C / EBP alpha decreases more than twofold during the mid to late G 1 and S phase ( 8 24 h after hepatectomy ) coordinately with a threefold increase in expression of C / EBP beta . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Since liver tissue contains high levels of the bZIP factors , liver enriched activating protein ( LAP ) , the CCAAT / enhancer binding protein alpha ( C / EBP alpha ) , and the D element binding protein ( DBP ) , and since P 1 contains a functional C / EBP alpha binding site ( P 1 CBS ) , we have examined the role of these transcription factors in the activation of IGF 2 P 1 . ^^^ Transient transfection experiments reveal that P 1 can be activated up to 15 fold by LAP and up to 6 fold by C / EBP alpha but can not be activated by DBP . ^^^ Electrophoretic mobility shift assays with liver nuclear extract show that P 1 CBS is predominantly bound by LAP and C / EBP alpha . ^^^ The P 1 CBS element is a high affinity binding site , which was demonstrated by comparing P 1 CBS with other LAP C / EBP alpha binding sites employing quantitative electrophoretic mobility shift assay . ^^^ These results indicate that LAP and C / EBP alpha are major contributors to the postnatal liver specific activation of the human IGF 2 promoter P1 . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Expression of the liver enriched transcription factors C / EBP alpha , C / EBP beta , HNF 1 , and HNF 4 in preneoplastic nodules and hepatocellular carcinoma in rat liver . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Members of the C / EBP alpha and C / EBP beta , but not C / EBP delta , family are able to bind to these two sites . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Further characterization has revealed the activation and interaction of C / EBP alpha , C / EBP beta ( NF IL 6 ) , NFKB 1 ( p 50 ) , and RelA ( p 65 ) to their specific binding elements present in the bovine IL 6 gene . ^^^ These results suggest a model in which induction of C / EBP alpha in differentiating monocytes contributes and synergizes with induced C / EBP beta and NF kappa B , which are activated following LPS stimulation , to mediate a high rate of IL 6 transcription under inflammatory conditions . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
During the acute phase response , individual C / EBP family members are discordinately regulated : C / EBP alpha levels fall , whereas another C / EBP family member termed nuclear factor IL 6 ( NF IL 6 ) , is induced . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Expression of the insulin like growth factor 1 gene is stimulated by the liver enriched transcription factors C / EBP alpha and LAP . ^^^ In this report we show , employing transient transfection experiments in Hep3B cells , that two liver enriched transcription factors , CCAAT / enhancer binding protein alpha ( C / EBP alpha ) and liver enriched activating protein ( LAP ) , enhance the activity of IGF 1 P 1 . ^^^ Mutations of the C / EBP LAP binding site completely abolished the enhancing effect of C / EBP alpha and LAP , indicating that their activating signal is indeed conferred by this binding site . ^^^ These results suggest that both C / EBP alpha and LAP play important roles in the liver specific expression of the hIGF 1 gene and provide the first clues in the elucidation of its complicated developmental stage and tissue specific expression pattern . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Only HNF 1 alpha , and not HNF 1 beta , HNF 4 , C / EBP alpha , C / EBP beta , or DBP , was able to activate the CYP2E1 promoter . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The hypoxia enhanced band on EMSA displayed a supershift with antibody to CCAAT enhancer binding protein beta ( C / EBP beta ) , but antibody to C / EBP alpha or delta was without effect . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
By using electrophoretic mobility shift assays with nuclear extracts and purified transcription factors , and antibody supershift assays we were able to characterize four liver enriched factors and one ubiquitous transcription factor interacting with the proximal promoter binding sites ( sites A to E ) : hepatocyte nuclear factor ( HNF ) 1 ( site A ) , NF kappa B ( site B ) , C / EBP alpha and C / EBP beta ( proximal and distal regions of site C , and site D ) , and HNF 4 ( site E ) . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Both C / EBP alpha and C / EBP beta were shown to contribute to the binding activity with the contribution by C / EBP beta increasing during the APR . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Gel shift analysis revealed that 6 h of insulin treatment decreased the binding of nuclear C / EBP alpha while increasing binding of nuclear C / EBP beta and C / EBP delta . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Neither C / EBP alpha nor C / EBP beta ( NF Il 6 ) binds the human C / EBP alpha promoter within 437 bp . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
In addition to transactivating liver specific genes , LAP , but not C / EBP alpha , arrests the cell cycle before the G1 / S boundary in hepatoma cells . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Nuclear expression of the 36 kilodalton C / EBP delta protein increases immediately after birth , followed first by increases in the 38 kilodalton C / EBP beta protein expression and then by increases in the 42 kilodalton C / EBP alpha protein expression . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
C / EBP beta activated the TNF alpha gene promoter in cotransfection assays and bound to it at a site which failed to bind the closely related protein C / EBP alpha . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The abundance of C / EBP alpha and C / EBP beta proteins in adult brown fat is similar to that found in adult liver . ^^^ However , the expression of C / EBP alpha and C / EBP beta is specifically regulated in brown fat during development . ^^^ C / EBP alpha , 30 kDa C / EBP alpha , C / EBP beta and LIP content is several fold higher in fetal brown fat than in the adult tissue , or liver at any stage of development . ^^^ When adult rats are exposed to a cold environment , which is a physiological stimulus of brown adipose tissue hyperplasia and UCP gene expression , a specific rise in C / EBP beta expression with respect to C / EBP alpha , 30 kDa C / EBP alpha and LIP is observed . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The ability to promote the adipogenic program requires the potent transcriptional activation domain of C / EBP alpha and is not observed with C / EBP beta . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
To study further roles of NF kappa B and C / EBP transcription factor family members in SAA 3 gene activation , expression vectors for NF kappa B subunits ( p 50 and p 65 ) and C / EBP family members ( C / EBP alpha and NFIL 6 , also called C / EBP beta ) were co transfected into Hep3B hepatoma and F 9 embryonic carcinoma cells . ^^^ The results show that , while the expression of p 65 alone strongly transactivated a SAA 3 gene , p 50 did not induce a significant transactivation , and NFIL 6 and C / EBP alpha induced only a marginal transactivation when expressed alone . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
After confluence , insulin induced the expression of C / EBP alpha and delta , whereas the expression of C / EBP beta remained essentially unmodified . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
As shown by the footprinting and band shift assays , the transcription factors C / EBP alpha and C / EBP beta can bind to elements C and D but the dissociation constant ( Kd ) of C / EBP alpha is 10 times lower for element C ( 0 . 6 nM ) than for element D ( 5 . 8 nM ) . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
C / EBP alpha , C / EBP beta , and C / EBP delta were capable of interacting with elements A and B . ^^^ Under noninduced conditions , C / EBP alpha was the major binding factor ; however , upon cytokine stimulation C / EBP beta and C / EBP delta binding activities were dramatically increased and became the predominant binding factors . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
In gel mobility assays , antibodies to both C / EBP beta and the cAMP regulatory element binding protein ( CREB ) , but not to C / EBP alpha , `` supershifted ' ' DNA protein complexes formed between a synthetic CRE oligomer and proteins prepared from rat liver nuclei . ^^^ C / EBP beta mRNA was expressed at low levels in both the periportal and pericentral regions of the liver lobule , whereas expression of the gene for C / EBP alpha was confined to the pericentral region of the liver lobule . ^^^ C / EBP beta and CREB bound to the CRE with similar affinities , both of which were greater than the affinity of C / EBP alpha . ^^^ Within 90 min after the administration of dibutyryl cAMP to rats , there was a marked increase in the hepatic concentration of C / EBP beta mRNA and a decrease in the level of mRNA for C / EBP alpha . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Neonatal mouse liver expresses C / EBP alpha , C / EBP beta and C / EBP delta mRNAs . ^^^ In contrast neonatal mouse brain expresses high levels of C / EBP delta , but little C / EBP alpha or C / EBP beta mRNA . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Class 3 genes [ gadd 153 , beta actin , ubiquitin ( UbC ) , phosphoglycerate kinase 1 , C / EBP alpha , C / EBP beta ] exhibit increased expression in response to amino acid limitation . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
In addition we show that both C / EBP alpha and the C / EBP beta can transactivate the human insulin receptor promoter in a dose dependent manner . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Activity was markedly increased by cotransfection with a vector expressing C / EBP beta but was unaffected by vectors producing other liver enriched transcription factors ( C / EBP alpha , HNF 1 alpha , and DBP ) . ^^^ C / EBP alpha was unable to substitute for C / EBP beta in forming this ternary complex . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Regenerative changes in C / EBP alpha and C / EBP beta expression modulate binding to the C / EBP site in the c fos promoter . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Two copies of each site placed upstream of the enhancerless SV 40 promoter confer C / EBP alpha and C / EBP beta responsiveness to this heterologous promoter when co transfected into HepG 2 cells . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The C / EBP family consist of several related proteins , C / EBP alpha , C / EBP beta , C / EBP gamma , and C / EBP delta , that form homodimers and that form heterodimers with each other . ^^^ NF kappa B p 65 , p 50 , and Rel functionally synergize with C / EBP alpha , C / EBP beta , and C / EBP delta . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Adhesion of hepatocytes to the EHS gel for the same period of time dramatically alters this program : it arrests growth and inhibits AP 1 DNA binding activity and the expression of c jun , junB , and c myc mRNAs , but , in addition , it restores C / EBP alpha mRNA and protein as well as C / EBP alpha and C / EBP beta DNA binding activities to the abundant levels present in freshly isolated hepatocytes . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Furthermore , transient expression of C / EBP alpha and to a lesser extent C / EBP beta expression vectors , results in transactivation of a cotransfected C / EBP alpha promoter luciferase reporter construct . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
In the nuclear extracts from normal liver , C / EBP alpha was the dominant form that bound to GPS 1 , whereas both C / EBP alpha and C / EBP beta bound to GPS 1 in the nuclear extracts from carcinogenic liver . ^^^ Furthermore , transfection assays showed that C / EBP alpha not only repressed the GST P promoter activity but also attenuated the transcriptional stimulation by C / EBP beta . ^^^ These observations strongly suggest that the ratio of C / EBP alpha to C / EBP beta is one of the important factors for the GST P silencer activity , and the decrease of this ratio during hepatocarcinogenesis reduces the silencer activity and , consequently , increases the GST P expression . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Using monospecific antibodies we demonstrate that three isoforms of the CCAAT / enhancer binding protein ( C / EBP ) family , namely C / EBP alpha , C / EBP beta , and C / EBP delta , bind to the APRE . ^^^ Furthermore , with liver nuclear protein from control animals , C / EPB alpha is the predominant form that binds to the APRE , whereas with nuclear proteins from acute phase induced animals , C / EBP alpha is replaced by C / EBP beta . ^^^ The mechanism of activation of the AGP gene during the acute phase response appears to involve an exchange of C / EBP alpha by C / EBP beta . ^^^ Finally , functional analyses indicate that C / EBP alpha , C / EBP beta , and C / EBP delta are strong transcriptional trans activators of the AGP APRE in hepatoma cells . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
To assess whether these factors might be involved in the hepatocyte proliferation program , we have studied the expression of the three C / EBP isoforms C / EBP alpha , C / EBP beta , and C / EBP delta and the two hepatocyte enriched transcription factors , HNF 1 and HNF 4 , in rat liver at various time points after partial hepatectomy and sham operations using transcriptional `` run on ' ' assays and Northern blot and Western blot experiments . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Differential adrenergic regulation of C / EBP alpha and C / EBP beta in brown adipose tissue . ^^^ We investigated the regulation of the expression of two members of the C / EBP family of transcriptional activators , C / EBP alpha and C / EBP beta , in brown adipose tissue in mice . ^^^ C / EBP alpha steady state mRNA and protein levels were drastically and rapidly reduced whereas C / EBP beta mRNA and protein levels were induced severalfold . ^^^ Preconfluent cells in brown fat primary cultures responded to norepinephrine with a decrease in C / EBP alpha and an increase in C / EBP beta mRNA ; in confluent cells the expression of both factors was increased . ^^^ Thus , C / EBP alpha and C / EBP beta gene expression is under adrenergic control both in vivo and in vitro but the type of response is directed by the degree of differentiation of the cells . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Analysis of mRNA for three isoforms of the CCAAT / enhancer binding protein ( C / EBP ) revealed that TCDD ( 5 nM ) selectively inhibited the induction of C / EBP alpha mRNA but did not block the induction of C / EBP beta and C / EBP delta in response to differentiation inducers . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Effect of thermal injury on the expression of transcription factors that regulate acute phase response genes : the response of C / EBP alpha , C / EBP beta , and C / EBP delta to thermal injury . ^^^ Northern blot hybridization was performed with 32P labeled C / EBP alpha , C / EBP beta , and C / EBP delta cDNAs . ^^^ A simultaneous decrease in C / EBP alpha and an increase in C / EBP beta and C / EBP delta mRNA levels occur in response to thermal injury . ^^^ Western analyses detect changes in C / EBP alpha and C / EBP beta pool levels that suggest a differential regulation of these genes in response to thermal injury . ^^^ The responses of C / EBP alpha , C / EBP beta , and C / EBP delta are similar in Buffalo , Sprague Dawley , and Fischer rats . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Whereas C / EBP alpha expression is not regulated by glucocorticoids , C / EBP beta and delta mRNA and protein levels are rapidly induced . ^^^ Moreover , C / EBP beta and delta containing DNA binding complexes are increased by glucocorticoids as determined by supershift assays , in contrast to C / EBP alpha containing complexes . ^^^ Immunofluorescence studies show cytoplasmic and nuclear localization for C / EBP alpha , in contrast to a restricted nuclear localization for both C / EBP beta and C / EBP delta . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The leucine zipper transcription factors C / EBP alpha and C / EBP beta exhibit growth related variations of expression and DNA binding during liver regeneration . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
These mutations abolished the binding of rat liver nuclear activities as well as transcription factors C / EBP alpha , C / EBP beta , and C / EBP delta expressed in COS 1 cell lysates to element D . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
To date , we have only found evidence for involvement of C / EBP alpha , although further experiments will be needed to exclude the role of C / EBP beta and C / EBP delta in receptor gene expression . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Using gel mobility supershift assays , this element was demonstrated to bind C / EBP alpha and C / EBP beta in liver nuclear extracts and in lysates from hepatocytes cultured on Matrigel . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Unexpectedly , a significant activation of 3xNF kappaBCAT was also seen upon its co transfection with the expression vector for CCAAT box enhancer binding protein alpha ( C / EBP alpha ) ( but not C / EBP beta or C / EBP delta ) . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
In contrast , mRNA and whole cell protein concentrations of C / EBP beta and delta are not altered by TNF alpha exposure , and nuclear concentrations of another C / EBP isoform , C / EBP alpha , are decreased by 80 % . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
C / EBP beta and delta mRNA levels were enhanced by IL 1 beta , whereas that of C / EBP alpha was not affected by treatment with this interleukin in both normal and adrenalectomized rats . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
C / EBP beta gene expression is regulated only via beta receptors , but the expression of the C / EBP alpha gene shows a switch during differentiation : in young cells , the expression is represented through both beta and alpha 1 receptors ; in mature cells , the expression is stimulated via b receptors . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Supershift analysis with anti C / EBP antibodies revealed that the complexes formed consist of both C / EBP alpha and C / EBP beta . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Supershift assays using antibodies against C / EBP alpha and C / EBP beta and mutational analyses indicate that the protein binding to the C / EBP consensus recognition site at 925 to 933 is C / EBP alpha . ^^^ The activity of promoter / CAT constructs containing the C / EBP recognition site is significantly decreased by cotransfection of C / EBP alpha but not C / EBP alpha but not C / EBP beta into either DDT 1 MF 2 cells or primary rat hepatocytes . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Furthermore , all these changes seemed to correlate with the presence of fatty liver and the high serum free fatty acid levels , suggesting that disturbance of fatty acid metabolism affects nitrogen metabolism at least in part via altered gene expression of transcription factors such as HNF 4 , C / EBP alpha , and C / EBP beta . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
To determine which C / EBP isoform was required , Gal 4 fusion proteins were created that contained the Gal 4 DNA binding domain ligated to the transcriptional activation domain of C / EBP alpha , C / EBP beta or the cAMP responsive element binding protein . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Furthermore , TCDD did not change the mRNA or protein levels of C / EBP beta , which is thought to play a role in inducing C / EBP alpha and PPAR gamma 2 expression . ^^^ These results suggest that TCDD inhibited 3T3 L 1 preadipocyte differentiation through the AhR pathway , and the change of C / EBP beta mRNA and protein was not involved in reducing mRNA expression of C / EBP alpha and PPAR gamma 2 . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The ability of C / EBP beta but not C / EBP alpha to synergize with an Sp 1 protein is specified by the leucine zipper and activation domain . ^^^ Despite the fact that both C / EBP alpha and C / EBP beta are expressed abundantly in liver , only C / EBP beta is capable of stimulating the 2D5 promoter in HepG 2 hepatocarcinoma cells . ^^^ Domain switch experiments reveal that C / EBP beta proteins containing either the leucine zipper or the activation domain of C / EBP alpha are unable to stimulate the 2D5 promoter yet are fully capable of transactivating an artificial promoter bearing a high affinity C / EBP site . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Congenital hypothyroidism caused a significant decrease in both C / EBP alpha and C / EBP beta gene expression at early stages of postnatal development . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
C / EBP beta , C / EBP alpha , and C / EBP delta , which bind to element C , prevented the inhibition of promoter activity . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Since C / EBP beta proteins were affected to a much lesser extent , the C / EBP alpha : C / EBP beta ratio was greater in the presence of EHS gel . ^^^ Thus stabilized C / EBP alpha expression , an increased C / EBP alpha : C / EBP beta ratio , and increased expression of liver specific mRNAs all correlated with the transition of proliferative to differentiated cells . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Changes in expression of CCAAT / enhancer binding protein alpha ( C / EBP alpha ) and C / EBP beta in rat liver after partial hepatectomy but not after treatment with cyproterone acetate . ^^^ We have investigated the effects of the liver growth promoter cyproterone acetate on the hepatic expression of C / EBP alpha and C / EBP beta . ^^^ Livers were obtained at different time intervals for measurement of C / EBP alpha and C / EBP beta mRNA with solution hybridization / RNAse protection assay , and C / EBP alpha and C / EBP beta content with immunoblotting . ^^^ RESULTS : The levels of both C / EBP alpha and C / EBP beta mRNA and the corresponding immunoreactivities were unchanged 2 48 h after injection of cyproterone acetate . ^^^ CONCLUSIONS : Liver cell proliferation during regeneration , but not in response to cyproterone acetate treatment , is associated with changes in C / EBP alpha and C / EBP beta expression . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The NF IL 6 like complex contains subunit C / EBP beta ( NF IL 6alpha ) as one of its components , but apparently not C / EBP alpha or C / EBP delta ( NF IL 6beta ) . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
In contrast to mammary gland , C / EBP alpha is the predominate isoform expressed in liver with relatively low expression of C / EBP beta and C / EBP delta mRNA . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
During hepatic proliferation , expression of hepatocyte nuclear factors ( HNF ) 1alpha , HNF 4 , C / EBP alpha , and C / EBP beta mRNAs was depressed , whereas that of HNF3alpha and HNF3beta transcripts was enhanced . ^^^ However , expression of C / EBP alpha and C / EBP beta mRNAs was partially recovered . ^^^ Thus , expression of HNF3gamma , C / EBP alpha , and C / EBP beta may be necessary for hepatocytes to acquire highly differentiated functions in addition to coexpression of certain amounts of transcripts of HNF1alpha , HNF1beta , HNF3alpha , HNF3beta , and HNF 4 as well as suppression of C / EBP delta . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Several high probability transcription factor recognition sequences , including proteins that are enriched in , or specific to , the liver , such as C / EBP alpha , C / EBP beta , HNF 1 , and HNF 3 beta , have been located in the 5 ' region of the gene . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The transcription factors CCAAT / enhancer binding proteins alpha and beta ( C / EBP alpha and C / EBP beta ) are highly expressed in liver and are believed to function in maintaining the differentiated state of the hepatocytes . ^^^ For three of the protein binding sites in the apoVLDL 2 promoter region , C / EBP alpha and C / EBP beta were identified as the major DNA binding activities . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
As with LPS , the C / EBP beta mRNA levels increased , while the C / EBP alpha mRNA levels decreased in response to LPS . ^^^ Protein pool levels and DNA binding activities of the 35 and 20 kDa C / EBP beta isoforms increase , whereas protein pool levels of the 42 kDa C / EBP alpha decrease and the 30kDa remained high . ^^^ These studies suggest that the synthesis of specific C / EBP alpha and C / EBP beta isoforms is induced by hyperthermia , and that the regulation of the AGP 1 and AGP 2 genes during heat stress may involve one of these isoforms . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
In postnatal adipose tissue C / EBP alpha and C / EBP beta protein were expressed in both 8 day old postnatal and mature ( 180 day ) pigs . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
In addition , C / EBP beta and C / EBP delta , but not C / EBP alpha , bind specifically to the C / EBP site of SAA 2 in response to IL 6 stimulation . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
An antibody for C / EBP alpha super shifted the nucleoprotein complex in THP 1 cells but not in the VD 3 treated HL 60 cells , whereas an antibody for C / EBP beta blocked the formation of the complex with the nuclear factor of the HL 60 cells but not with that of THP 1 cells . ^^^ Thus , it was concluded that C / EBP alpha and beta were able to bind to the C / EBP motif , and that C / EBP alpha bound to the motif in THP 1 cells and C / EBP beta bound to that in the VD 3 treated HL 60 cells . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
All three of these factors are expressed by bone marrow derived macrophages , with the DNA binding activity of C / EBP beta and delta increased by treatment with LPS while that of C / EBP alpha is decreased . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Transcription factors C / EBP alpha , C / EBP beta , and CHOP ( Gadd 153 ) expressed during the differentiation program of keratinocytes in vitro and in vivo . ^^^ Within this framework , western immunoblot analysis and immunohistochemistry reveal that C / EBP alpha increases 5 fold at 1 2 d and remains elevated , C / EBP beta rises 2 fold at 2 4 d and gradually falls , and CHOP rises 9 fold in the first 24 h then returns rapidly to baseline . ^^^ Several products of alternative translation are observed in BALB / MK cells , i . e . , 42 kDa and 30 kDa forms of C / EBP alpha , and 32 kDa and 20 kDa forms of C / EBP beta . ^^^ C / EBP alpha is concentrated in the upper epidermis in a predominantly cytoplasmic location within cells , whereas the highest levels of C / EBP beta and CHOP are seen in the mid epidermis , mainly within nuclei . ^^^ High levels of C / EBP beta and CHOP ( but not C / EBP alpha ) are also observed in hair follicles and sebaceous glands . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Instead , insulin may regulate GLUT 4 mRNA by inactivating C / EBP alpha through dephosphorylation as well as by inducing the expression of the dominant negative form of C / EBP beta ( liver inhibitory protein ) , since both of these processes occur in the presence of thiazolidinediones . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
To a lesser extent C / EBP alpha and delta also interacted with the NF IL 6 site . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Exposure of the cells to LPS , IL 1 , IFN gamma and TNF alpha produced a reduction of C / EBP alpha mRNA levels and a corresponding increase in the expression of C / EBP beta and C / EBP delta . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Activation by RB was mediated through a NF IL 6 ( C / EBP beta ) binding motif in the human SP D promoter , and this sequence specifically bound to C / EBP alpha , C / EBP beta , and C / EBP delta . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
We have identified and characterized putative zebrafish orthologs of mammalian C / EBP alpha , beta , gamma , and delta using low stringency hybridization screening and computer searches of the GenBank EST database . c / ebpa and g were mapped within 1 cM of each other on linkage group ( LG ) 7 , syntenic with human CEBPA and G genes on chromosome 19 . c / ebpb was mapped to LG 8 , and c / ebpd was mapped to LG 24 , on the same LG as a recently identified unique c / ebp in zebrafish , c / ebp1 . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Further analysis indicated that GR specifically potentiated the induction of transcription by C / EBP beta , but not C / EBP alpha or delta , and that full induction could be obtained by the ligand binding domain ( LBD ) of GR alone . ^^^ C / EBP beta , but not C / EBP alpha or delta , reciprocally potentiated transcriptional activation by DNA bound GR LBD . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
By immunohistochemistry , we demonstrated that C / EBP alpha and C / EBP beta were expressed in alveolar type 2 cells and alveolar macrophages , but C / EBP delta was expressed restrictedly in some of alveolar type 2 cells in a spatial pattern in the control lungs . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The sequence of 3 . 18 kb upstream of exon 1 reveals numerous consensus transcription factor binding sites , some of which were shown to be important in the positive and negative control of Aldh3a1 gene expression ; these include seven aromatic hydrocarbon response elements ( AHREs ) , an electrophile response element ( EPRE ) , and AP 1 , C / EBP beta , c / EBP alpha , NF kappaB , Sp 1 , and NF 1 putative binding sites . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Overexpression of C / EBP beta or C / EBP alpha repressed , whereas AhR enhanced , 1 . 6CAT reporter activity in cells treated with benzo ( a ) pyrene ( BaP ) . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
In epithelial lung cells the C / EBP alpha isoform seems to play a role in the regulation of surfactant proteins ( SP ) and Clara cell specific protein ( CCSP ) , whereas the roles of C / EBP beta and C / EBP delta are unclear . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Genomic DNA from 381 cancers and cell lines representing leukemias , lymphomas and a variety of solid tumors were examined for mutations of genes coding for the C / EBP beta , C / EBP alpha , PU . 1 , and AML 1 transcription factors using single strand conformation polymorphism ( SSCP ) and direct DNA sequencing . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Gel mobility shift analyses indicated that C / EBP beta present in HepG 2 nuclear extracts and C / EBP alpha and beta present in adult rat liver nuclear extracts bind to the C / EBP site . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Introduction of an inducible C / EBP alpha gene into the line revealed that conditional expression of C / EBP alpha induced the C / EBP family members C / EBP beta and C / EBP epsilon and subsequent granulocyte differentiation . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The overexpression of C / EBP beta and C / EBP delta but not C / EBP alpha caused 6 to 8 fold induction of MCP 1 promoter activity . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
We show that functionally related domains are present in C / EBP alpha , C / EBP beta , and C / EBP delta . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
HNF 4 alpha , HNF 6 , C / EBP alpha , C / EBP beta , and TO were well expressed in the cells treated with Matrigel . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
These mice express C / EBP alpha , C / EBP beta , and C / EBP delta in the bone marrow at levels similar to those of their wild type counterparts , suggesting a lack of functional redundancy among the family in vivo . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The glucocorticoid receptor ( GR ) , in the presence of its hormone ligand , inhibited the activation of the PEPCK C gene promoter by C / EBP alpha or C / EBP beta but not by the adipocyte specific peroxisome proliferator activated receptor gamma 2 . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Chromatin immunoprecipitation assays demonstrated that C / EBP alpha , delta , and epsilon bound to this region of the 5 ' untranslated region , whereas C / EBP beta does not bind even under conditions of overexpression and stimulation . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Antibody supershift experiments revealed that LPS induced C / EBP DNA binding activity depended on C / EBP beta and C / EBP delta but not C / EBP alpha , C / EBP epsilon or CBP / p300 . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
CCAAT / enhancer binding protein alpha ( C / EBP alpha ) and C / EBP beta , liver specific transcription factors , are involved in hepatocyte proliferation and differentiation . ^^^ The expression level of the C / EBP alpha and C / EBP beta genes were examined between tumor and non tumorous tissues of the same hepatocellular carcinoma patients with quantitative real time polymerase chain reactions . ^^^ The expression of both the C / EBP alpha and C / EBP beta genes was down regulated in the majority of the tumor specimens compared with corresponding non tumorous tissues . ^^^ Patients whose expression of either C / EBP alpha or C / EBP beta was higher in tumors than non tumorous tissues survived longer than those whose expression was lower in tumors . ^^^ Higher expression of C / EBP alpha or C / EBP beta in tumors was reversibly correlated with the tumor size and progression of the clinical stage . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
DNA binding analysis shows that C / EBP beta , C / EBP delta , and C / EBP alpha ( p 42 and p 30 forms ) can bind to satellite DNA as homo or heterocomplexes in vitro . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Western blot analysis revealed that Dlx 3 , CCAAT / enhancer binding protein alpha ( C / EBP alpha ) , and C / EBP beta were present in choriocarcinoma cells and isolated trophoblasts from term human placentas . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
This observation is not explained by increased p 21 ( CIP1 / Waf1 ) expression or lack of phosphorylation of external LAP , but LAP overexpression triggered a decreased C / EBP alpha / C / EBP alpha 30 ratio and a reduced basal c jun level in the liver . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Amlodipine dependent inhibition of cell proliferation was partially reversed by RU 486 at 10 ( 8 ) M ( 58 % + / 29 % ) , antisense oligonucleotides targeting C / EBP alpha ( 91 % + / 26 % ) , or antisense mRNAs targeting p 21 ( Waf1 / Cip1 ) ( 96 % + / 32 % , n=6 ) ; scrambled antisense oligonucleotides or those directed against C / EBP beta were ineffective . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The present results showed that the expression levels of C / EBP alpha and C / EBP beta genes declined in activated HSCs . ^^^ Over expression of C / EBP alpha gene in activated HSCs : ( 1 ) inhibited HSCs proliferation , extracellular matrix producing , alpha smooth muscle actin gene expression , and induced rebound of cytoplasmic lipid droplets ; ( 2 ) reduced retinoic acid receptor beta , C / EBP delta and beta gene expressions , but increased the active form C / EBP beta PSer ( 105 ) , and induced retinoid 10 receptor alpha gene expression ; and ( 3 ) did not affect the protein level of p16INK4a , p21Cip1 / WAF1 or p27Kip1 . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
DIPA interacts with C / EBP beta and delta proteins in intact cells and inhibits their transcriptional activity but not that of C / EBP alpha . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Whereas both Sp 1 and C / EBP beta transactivated hRFC C promoter activity , C / EBP alpha and gamma were transcriptionally inert . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Antisense oligonucleotide for C / EBP delta , but not C / EBP alpha or C / EBP beta , effectively suppressed IPA induced differentiation , suggesting that the expression of C / EBP delta protein is necessary for cytokinin induced differentiation . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
C / EBP alpha and C / EBP beta regulate haptoglobin gene expression during rat liver development and the acute phase response . ^^^ The participation of C / EBP alpha and C / EBP beta in the transcriptional regulation of the haptoglobin ( Hp ) gene throughout liver development and the acute phase ( AP ) response was examined . ^^^ Western immunoblot analysis revealed that the relative concentrations of C / EBP alpha and C / EBP beta increased during differentiation in two nuclear protein fractions the nuclear extract and nuclear matrix . ^^^ The AP reaction was accompanied by a decrease of the relative concentration of C / EBP alpha and an increase of C / EBP beta during development in both protein fractions . ^^^ These results indicate that Hp gene transcription is regulated by C / EBP alpha during normal liver development , whereas C / EBP beta is involved in the AP regulation during the later phase of differentiation and in the adult . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Hepatic CCAAT / enhancer binding protein ( C / EBP alpha and C / EBP beta ) expression changes with riboflavin deficiency , diet restriction and starvation in rats . ^^^ To study the role of nuclear regulatory proteins in mediating dietary effects , hepatic CCAAT / enhancer binding protein ( C / EBP ) , mRNA and transcription rate were measured for C / EBP alpha and C / EBP beta in nutritional states that profoundly alter energy metabolism and growth . ^^^ We investigated the mechanism responsible for increased protein by measuring steady state mRNA levels and transcription rates for C / EBP alpha and C / EBP beta . ^^^ We conclude that 1 ) C / EBP alpha and C / EBP beta expression responds to diet but may involve different dietary signals for diet restriction vs . riboflavin deficiency ; 2 ) the dietary regulation of C / EBP alpha and C / EBP beta expression seems to be controlled in part at the level of gene transcription ; and 3 ) C / EBP alpha and C / EBP beta nuclear proteins , by virtue of their increased quantities , may participate in regulating altered energy metabolism and growth by influencing hepatic transcription of key metabolic enzymes . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Analysis of 3 . 2kb of the CTGF / CCN2 proximal promoter sequence revealed a consensus TATAA box , putative AP 1 , Brn 2 , CdxA , C / EBP alpha , C / EBP beta , C Ets , delta E , HFH 2 , and HSF 2 binding sites . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NF IL 6 , a member of the C / EBP family , regulates E1A responsive promoters in the absence of E1A . ^^^ A cDNA encoding NF IL 6 , an interleukin 6 ( IL 6 ) regulated human nuclear factor of the C / EBP family , is demonstrated to complement the transactivation function of E1A . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
C / EBP alpha at the composite element plays an essential role in the VDR regulation of SULT2A1 , because 1 ) induction was lost for promoters with inactivating mutations at the VDRE or C / EBP element ; 2 ) SULT2A1 induction by 1 , 25 ( OH ) 2D3 in C / EBP alpha deficient cells required the expression of cotransfected C / EBP alpha ; and 3 ) C / EBP beta did not substitute for C / EBP alpha in this regulation . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Activation of CCAAT / enhancer binding protein ( C / EBP ) alpha expression by C / EBP beta during adipogenesis requires a peroxisome proliferator activated receptor gamma associated repression of HDAC 1 at the C / ebp alpha gene promoter . ^^^ Ectopic expression of C / EBP beta in Swiss 3T3 mouse fibroblasts ( Swiss LAP cells ) induces PPARgamma expression without any significant enhancement of C / EBP alpha expression . ^^^ To further establish a role for PPARgamma in regulating C / EBP alpha expression , we expressed C / EBP beta in PPARgamma deficient mouse embryo fibroblasts ( MEFs ) . ^^^ The data show that C / EBP beta is capable of inducing PPARgamma in Ppar gamma+ / MEFs , which leads to activation of adipogenesis , including C / EBP alpha expression following exposure to a PPARgamma ligand . ^^^ In contrast , C / EBP beta is not able to induce C / EBP alpha expression or adipogenesis in Ppar gamma / MEFs . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Although five C / EBP family proteins are known which bind the E site , antibody ablation of DNA : protein complexes resolved by electrophoretic mobility shift assays showed that Ig / EBP 1 is the only E site binding protein detectable in early B cell lines ; more mature B cells contain Ig / EBP 1 and NF IL 6 . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Calcium regulated phosphorylation within the leucine zipper of C / EBP beta . ^^^ Here , a pathway for calcium mediated signals is demonstrated that involves C / EBP beta , a member of the bZip family of transcription factors . ^^^ In pituitary cells C / EBP beta was phosphorylated in response to increased intracellular calcium concentrations as a consequence of the activation of a calcium calmodulin dependent protein kinase . ^^^ Phosphorylation of serine at position 276 within the leucine zipper of C / EBP beta appeared to confer calcium regulated transcriptional stimulation of a promoter that contained binding sites for C / EBP beta . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The steady state levels of C / EBP beta and a new isoform , C / EBP delta , were dramatically increased in many tissues within 4 h following LPS treatment . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
However , the effect of insulin on C / EBP beta was unexpected and paradoxical : while insulin completely inhibited the transcriptional activation of the C / EBP beta gene in cytokine and dexamethasone treated cells , the level of cytoplasmic C / EBP beta RNA was elevated . ^^^ Quantitation of C / EBP beta mRNA by Northern ( RNA ) blot analysis and of C / EBP beta DNA binding activity by Southwestern ( DNA protein ) blot analysis showed that insulin , when combined with cytokines and dexamethasone , stimulated both the mRNA and DNA binding activity by a factor of 1 . 6 compared with that of cells treated with cytokines and dexamethasone alone . ^^^ Transient transfection of H 35 and HepG 2 cells with a chloramphenicol acetyltransferase ( CAT ) gene expression vector containing the C / EBP beta response element also resulted in a 1 . 5 fold increase of C / EBP beta mediated transcription in insulin treated cells . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Direct cloning of NF IL 6 revealed its homology with C / EBP . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
C / EBP beta was found to be the principal isoform in hepatoma cells and to be strongly stimulated by cytokines and dexamethasone in H 35 cells . ^^^ Transcription run on reactions with nuclei from transiently transfected H 35 cells indicated that cotransfected C / EBP beta increases basal expression of reporter gene constructs as well as the dexamethasone mediated stimulation of constructs containing the glucocorticoid response elements of the rat alpha 1 acid glycoprotein gene , but did not accelerate or enhance hormone dependent transcription activation of reporter gene plasmids containing the IL 6 regulatory element of the beta fibrinogen gene . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Direct cloning of the human NF IL 6 revealed its similarity to C / EBP , a liver and adipose tissue specific transcription factor . ^^^ C / EBP and NF IL 6 recognize the same nucleotide sequence , but exhibit distinct patterns of expression . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The sequence of this region bears significant similarity to binding sites of members of the bZIP family of liver enriched or specific factors such as C / EBP , DBP , and LAP , that are characteristically produced relatively late during liver development . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
CHOP , a novel developmentally regulated nuclear protein that dimerizes with transcription factors C / EBP and LAP and functions as a dominant negative inhibitor of gene transcription . ^^^ We report on the identification of a nuclear protein that serves as a dominant negative inhibitor of the transcription factors C / EBP and LAP . ^^^ A 32P labeled LAP DNA binding and dimerization domain `` zipper probe ' ' was used to isolate a clone that encodes a new C / EBP homologous protein : CHOP 10 . ^^^ Consistent with the structure of its defective basic region , bacterially expressed CHOP 10 inhibits the DNA binding activity of C / EBP and LAP by forming heterodimers that can not bind DNA . ^^^ In transfected HepG 2 cells , expression of CHOP 10 attenuates activation of C / EBP and LAP driven promoters . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
We now demonstrate that another transcription factor , the liver activator protein ( LAP ) , also interacts with the same region of the promoter based on the following observations : ( 1 ) LAP synthesized by in vitro transcription and translation of cloned cDNA sequence forms complexes with an oligonucleotide containing the C / EBP binding sequence within the ADH promoter as determined by the electrophoretic mobility shift assay ( EMSA ) , ( 2 ) purified LAP interacts with the proximal ADH promoter when analyzed by the DNase 1 protection assay , and ( 3 ) an ADH promoter reporter gene construct containing the C / EBP binding site is transactivated by an eukaryotic expression vector containing the LAP sequence . ^^^ Additional competition experiments with the ADH or 422 ( aP 2 ) oligonucleotide using either RLNE or extracts from 3T3 L 1 adipocytes demonstrated that complex 1 contains either C / EBP or LAP , while complex 2 contains a DNA binding protein that binds to a novel sequence 5 ' TGGCCCAGTT 3 ' between positions 1 and 10 of the ADH promoter . ^^^ Ultraviolet cross linking between RLNE and a labeled oligonucleotide containing the above sequence indicates that this protein , designated EDBP ( for enhancer site downstream binding protein ) , has an estimated molecular weight of 47 kDa , which is larger than that reported for either C / EBP ( 42 kDa ) or LAP ( 36 kDa ) . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
A nuclear factor for controlling IL 6 gene expression ( NF IL 6 ) is a leucine zipper containing transcription factor and is homologous to C / EBP , a liver nuclear factor . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Reciprocal expression of NF IL 6 and C / EBP in hepatocytes : possible involvement of NF IL 6 in acute phase protein gene expression . ^^^ The recently cloned nuclear factor NF IL 6 , a potent trans acting regulator of IL 6 gene expression , has a region that is highly homologous to the liver specific transcriptional factor C / EBP . ^^^ The ability of NF IL 6 to replace C / EBP may explain the positive and negative acute phase responses induced by IL 6 . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Clone 21 encodes a b zip domain polypeptide that is related to the liver transcription factor C / EBP , and is homologous to the human NFIL 6 transcription factor ( called here rNFIL 6 ) . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
In addition to a canonical TATA box located 31 base pairs upstream of the start site of transcription , putative binding sites for the liver enriched and liver specific transcription factors HNF1 / LF B1 / APF , HNF 3 , HNF4 / AF 1 , C / EBP , LAP , and eH TF / TGT3 and the ubiquitous factors NF 1 and OTF 1 were identified . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Three genes were isolated that encode bZIP DNA binding proteins ( designated CRP 1 , CRP 2 , and CRP 3 ) with strong amino acid sequence similarities to the C / EBP binding domain . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
A nuclear factor for IL 6 expression ( NF IL 6 ) is a member of a C / EBP family . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
IL 6DBP , a nuclear protein involved in interleukin 6 signal transduction , defines a new family of leucine zipper proteins related to C / EBP . ^^^ A cDNA clone coding for a member of the family , IL 6DBP , was isolated ; it is strongly homologous to C / EBP in the region of the basic domain and in the leucine zipper sequence . ^^^ IL 6DBP and C / EBP can interact in vitro to form heterodimers that bind to DNA with the same specificity as the respective homodimers , and they can interact functionally in vivo . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NF IL 6 was shown to be a member of a C / EBP family , and the possible involvement of NF IL 6 not only in the IL 6 regulation but also in the induction of various acute phase proteins was also observed . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
In this study , we purify and identify a C / EBP family member , AGP / EBP ( LAP ) , present in the rat hepatoma cell line HTC ( JZ . 1 ) which also binds to the C / EBP recognition sites in this region . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Direct cloning of NF IL 6 showed its homology with C / EBP , a hepatocyte and adipocyte specific transcription factor . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
A member of the C / EBP family , NF IL 6 beta , forms a heterodimer and transcriptionally synergizes with NF IL 6 . ^^^ This intronless gene , termed NF IL 6 beta encodes a 269 amino acid protein with a potential leucine zipper structure , and the gene product can bind to the CCAAT homology as well as the viral enhancer core sequence , as in the cases of NF IL 6 and C / EBP . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
LAP , a novel member of the C / EBP gene family , encodes a liver enriched transcriptional activator protein . ^^^ LAP shares extensive sequence homology ( 71 % ) in its DNA binding and leucine zipper domains with C / EBP . ^^^ In addition , both genes , lap and cebp , are devoid of intervening sequences . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The genetic program undertaken during the clonal expansion phase of 3T3 L 1 differentiation , controlled in part by C / EBP beta and C / EBP delta , is less clearly understood . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The expression of three C / EBP beta isoforms [ the liver enriched activating proteins ( LAPs ) ; and the liver enriched inhibiting protein ( LIP ) ] is elevated throughout pregnancy , with LIP expression increasing more than 100 fold . ^^^ C / EBP beta expression decreases at parturition , with LIP diminishing to levels observed in the virgin gland . ^^^ Therefore , glucocorticoids may impinge upon beta casein gene expression by altering the ratio of the inhibitory to the activating isoforms of C / EBP beta . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Expression of HNF 3 alpha , beta , and gamma , and C / EBP beta mRNA was highly activated in proliferating liver epithelial cells on days 2 and 3 after GalN injury . ^^^ We suggest that C / EBP delta could be involved in the initial activation of epithelial progenitor cells and that HNF 3 alpha , beta , and gamma , and C / EBP beta might participate in their maturation . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Conditional ectopic expression of C / EBP beta in NIH 3T3 cells induces PPAR gamma and stimulates adipogenesis . ^^^ Activation of adipogenesis in 3T3 preadipocytes by exposure to the adipogenic inducers dexamethasone , methylisobutylxanthine , insulin , and fetal bovine serum is accompanied by a transient burst of C / EBP beta protein expression that precedes the induction of the fat gene program . ^^^ In this study we have investigated the role of C / EBP beta in initiating the adipogenic program by overexpressing C / EBP beta in multipotential NIH 3T3 fibroblasts . ^^^ Conditional ectopic expression of C / EBP beta was accomplished by using an artificial transcriptional regulatory system based on the Escherichia coli tetracycline repressor to generate a stable cell line , beta 2 , that expresses C / EBP beta mRNA and protein in a tightly controlled tetracycline dose dependent manner . ^^^ Induction of C / EBP beta DNA binding activity in NIH 3T3 beta 2 cells exposed to dexamethasone in the presence of insulin and fetal bovine serum activates the expression of an adipocyte specific nuclear hormone receptor , PPAR gamma , that stimulates the conversion of these fibroblasts into committed preadipocytes . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
To identify transcription factors binding to PRE 1 , we screened a cDNA expression library from Jurkat T cells and isolated a cDNA encoding nuclear factor ( NF ) IL 6 ( also known as C / EBP beta ) . ^^^ Some of these complexes were demonstrated to contain NF IL 6 by using anti C / EBP beta Abs . ^^^ Promoter deletion studies revealed two additional NF IL 6 binding sites located at positions 44 to 36 ( C / EBP proximal ) and 87 to 79 ( C / EBP medial ) , respectively . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
AGP / EBP ( C / EBP beta ) is a key transcription factor responsible for transcriptional induction of many acute phase protein genes . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Increased c fos , c jun , C / EBP beta , and C / EBP delta mRNA expression was correlated with increased DNA binding activity of the transcription factor complexes activator protein 1 and C / EBP in tissue lysates . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The increase in ADH mRNA was preceded by an increase in the expression of C / EBP beta mRNA and in C / EBP beta protein . ^^^ These results show that cyclic AMP induces ADH and suggests that this effect is mediated by C / EBP beta binding to the C / EBP site . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
In all these clones , the tTA trans activator not only modulates the activity of the luciferase gene , but also modulates the activity of a number of endogenous proteins , including C / EBP beta , the glucocorticoid receptor ( GR ) , and SP 1 . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Repression of the interleukin 6 promoter by estrogen receptor is mediated by NF kappa B and C / EBP beta . ^^^ We show here that NF kappa B and C / EBP beta are important regulators of IL 6 gene expression in human osteoblasts . ^^^ This inhibition is mediated by the transcription factors NF kappa B and C / EBP beta . ^^^ The physical and functional interaction depends in part on the DNA binding domain and region D of ER and on the Rel homology domain of NF kappa B and the bZIP region of C / EBP beta . ^^^ The cross coupling between ER , NF kappa B , and C / EBP beta also results in reduced activity of promoters with ER binding sites . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Normal fibroblasts induce the C / EBP beta and ATF 4 bZIP transcription factors in response to anoxia . ^^^ In the case of C / EBP beta this was apparently related to the failure to detect an increase in the DNA binding of the induced factor to its consensus binding site . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Here , we investigated the tissue specific regulation of the mim 1 promoter and found that it not only contains binding sites for Myb but also for NF M , a myeloid specific transcription factor that probably corresponds to mammalian C / EBP beta . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
A potential regulatory role is attributed to the transcription factors C / EBP beta and NF kB based on studies on transiently transfected H 35 cells . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Sequence analysis of the clone revealed that the insert of lambda 1 2 encodes a part of the amino acid sequence of NF IL 6 ( also termed as LAP and C / EBP beta ) . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
In nuclear extracts from mammary epithelial cells , the prevalent C / EBP isoform binding to these sites is beta ( C / EBP beta ) . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The deduced amino acid sequence of the insert is identical to that corresponding to the DNA binding domain and the dimerization domain of a transcription factor , nuclear factor interleukin 6 ( NF IL 6 ) , a member of the CCAAT / enhancer binding protein ( C / EBP ) family . ^^^ Studies using specific antibodies against members of the C / EBP family demonstrate that NF IL 6 is the major nuclear factor binding to the regulatory element in BeWo cells ; nevertheless . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Northern ( RNA ) blots and binding assays showed that NF IL 6 is the only known C / EBP family member which is increased when U 937 cells are activated . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
LAP / NF IL 6 is a member of the C / EBP family of transcriptional activators and has been shown to be involved in the regulation of the acute phase response . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
CAAT box / enhancer binding protein beta ( C / EBP beta ) and C / EBP related activating transcription factor bind to the CRE in the promoter of the somatostatin gene and transactivate transcription . ^^^ CREB binds competitively with C / EBP beta to the somatostatin CRE in vitro and represses C / EBP beta induced transcription of the CRE containing somatostatin chloramphenicol acetyltransferase reporter . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
A computer homology search for a 1 . 5 kb promoter region showed that there are several consensus cis DNA elements such as C / EBP , NF IL 6 , and AP 1 in this region . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Lymphoproliferative disorder and imbalanced T helper response in C / EBP beta deficient mice . ^^^ C / EBP beta is considered a key element of interleukin 6 ( IL 6 ) signalling as well as an important transcriptional regulator of the IL 6 gene itself . ^^^ We describe here how mice lacking C / EBP beta develop a pathology similar to mice overexpressing IL 6 and nearly identical to multicentric Castleman ' s disease in human patients , with marked splenomegaly , peripheral lymphadenopathy and enhanced haemopoiesis . ^^^ Our data show that C / EBP beta is crucial for the correct functional regulation and homeostatic control of haemopoietic and lymphoid compartments . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Region 1 was found to be bipartite and to interact with both the CCAAT / enhancer binding protein ( C / EBP ) and the liver activator protein ( LAP ) between position 10 and 22 , while a different protein designated EDBP ( for Enhancer site Downstream Binding Protein ) bound between position 1 and 10 , a sequence previously not known to interact with any transcription factors . ^^^ C / EBP , LAP , and USF were all found to activate the alcohol dehydrogenase promoter in cotransfection experiments . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Early responses of trans activating factors to growth hormone in preadipocytes : differential regulation of CCAAT enhancer binding protein beta ( C / EBP beta ) and C / EBP delta . ^^^ Using specific antibodies in electrophoretic mobility shift assays and Western blots , it was shown that the increase in activity of C / EBP is the result of an increase in synthesis of two alternatively translated forms of C / EBP beta : 40 C / EBP beta and 23 C / EBP beta . ^^^ Concomitant with the translationally activated increase in C / EBP beta , a GH dependent increase was observed in C / EBP delta transcription . ^^^ This was accompanied by an increase in mRNA for C / EBP delta , which was superinduced by cycloheximide and , unlike the increase in C / EBP beta protein , was not observed with insulin . ^^^ Thus GH exerts its effects on C / EBP isoforms at two levels : transcriptional activation of C / EBP delta and translational activation of C / EBP beta . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NF IL 6 , a member of the C / EBP family of transcription factors , binds and trans activates the human MDR 1 gene promoter . ^^^ The nuclear protein was identified , using a HeLa lambda gt 11 cDNA expression library , to be the transcriptional regulator nuclear factor for interleukin 6 ( NF IL 6 ) , a member of the C / EBP family of transcription factors that bound an NF IL 6 like consensus element 5 ' TTTCGCAGT 3 ' . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The promoter region lacks an apparent TATA or CCAAT box but contains multiple putative interleukin response elements [ six NF IL 6 ( C / EBP beta ) and four APRF ( STAT 3 ) binding motifs ] , supporting the notion that interleukins may mediate labor induction via transcriptional activation of the OTR gene . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The two ccaat enhancer binding protein isoforms , IL 6DBP and C / EBP delta , are induced by interleukin 6 to promote gene transcription in hepatocytes via different mechanisms . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The presence of a similar , thusfar unnoticed , small ORF 5 ' to the major initiation codon of C / EBP beta mRNA suggests that start site multiplicity from this mRNA may be governed by the same mechanism . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The CCAAT / enhancer binding protein beta ( C / EBP beta ) is a transcription factor that is abundant in the liver . ^^^ The concentration of C / EBP beta mRNA in the liver of mice and rats fed a high carbohydrate diet , which causes a rise in blood insulin levels , was lower ( 80 and 65 % , respectively ) than that detected in animals fed a standard diet . ^^^ Similarly , the expression of the human insulin gene in the liver of transgenic mice led to a decrease in the concentration of C / EBP beta mRNA . ^^^ Furthermore , the expression of the C / EBP beta gene increased in the liver of diabetic rats and decreased in the liver of diabetic animals treated with vanadate , an insulin mimetic agent . ^^^ In addition , a decrease in C / EBP beta protein was observed in liver nuclei from mice after insulin injections , in mice fed a high carbohydrate diet , and in transgenic mice expressing the insulin gene in the liver . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Finally , evidence is presented showing that GABP beta 1 1 and GABP beta 2 1 can heterodimerize through this carboxy terminal domain , but neither protein can heterodimerize via the dimer forming region of the bZIP protein C / EBP beta . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Novel mechanism of C / EBP beta ( NF M ) transcriptional control : activation through derepression . ^^^ C / EBP beta is a transcription factor that becomes activated after phosphorylation to induce genes involved in inflammation , acute phase response , cytokine expression , cell growth , and differentiation . ^^^ We provide evidence that phosphorylation plays a unique role to derepress rather than to enhance the trans activation domain as a novel mechanism to regulate gene expression by NF M / C / EBP beta . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The importance of C / EBP proteins in B cell biology is suggested by the occurrence of functionally important C / EBP binding sites in Ig gene enhancers and promoters , and the knowledge that family member NF IL 6 is induced in other systems in response to regulators of B cell differentiation . ^^^ Two family members , Ig / EBP and NF IL 6 , seem to be the major regulators of C / EBP site dependent transcriptional activity in B cells . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Characterization of the NR 2 binding factor by heat stability and antibody supershifts in EMSA indicate that the factor is related to the CCAAT / enhancer binding protein ( C / EBP ) family , and that one component of the complexes may be C / EBP beta . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Multiple forms of C / EBP beta bind the EFII enhancer sequence in the Rous sarcoma virus long terminal repeat . ^^^ In this report we demonstrate that C / EBP beta is a major component of three EFII DNA binding complexes , EFIIa , EFIIb , and EFIIc , which we have previously shown to specifically recognize a C / EBP consensus binding site found in the EFII enhancer sequence from the Rous sarcoma virus long terminal repeat ( R . ^^^ Three different forms of C / EBP beta , p 42 , p 35 , and p 20 , can bind the EFII DNA sequence as homodimers , and dimerization experiments show that EFIIa is a homodimer of p 20 C / EBP beta , EFIIb is primarily composed of a p20 / p35 heterodimer with minor amounts of p20 / p42 heterodimer and p 35 homodimer , and EFIIc is composed of p 20 and / or p 35 heterodimerized with a novel 60 kDa protein . p 20 C / EBP beta is likely equivalent to the internally initiated translation product of C / EBP beta , LIP ( liver inhibitor protein ) , described by P . ^^^ In contrast to the low level of LIP expressed in liver , postulated to occur because of leaky ribosome scanning , we found high levels of expression of p 20 C / EBP beta in fibroblasts and lymphocytes . ^^^ In murine fibroblasts , p 20 C / EBP beta has an extended half life , four times longer than those of p 42 and p 35 C / EBP beta , which could contribute to its abundant accumulation in this cell type , even though its synthesis by leaky ribosome scanning might be inefficient . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Another is C / EBP beta , which also binds to three elements downstream from the GRE . ^^^ Binding experiments revealed that ANF 4 and C / EBP beta binding sites are partially overlapping and require the palindromic structure of the URE for high affinity binding . ^^^ We concluded that this transcription complex is composed of three distinct proteins , ANF 4 , C / EBP beta , and a 102 kD protein , and that they play an important role for the hormone regulation of AGP . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Human FUR promoter P 1 but not P1A or P1B was transactivated by transcription factor C / EBP beta . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The 5 ' flanking region of the rat LAP ( C / EBP beta ) gene can direct high level , position independent , copy number dependent expression in multiple tissues in transgenic mice . ^^^ We have discovered that a 2 . 8kbp fragment flanking the rat gene encoding the transcription factor LAP ( C / EBP beta ) directs position independent , copy number dependent expression in transgenic mouse livers . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Nuclear factor interleukin 6 ( NF IL 6 ) , a member of the CCAAT box / enhancer binding protein ( C / EBP ) family , contains a basic domain leucine zipper ( bZIP ) DNA binding motif . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Results of electrophoretic mobility shift assays indicated that the Hx IL 6 RE is a binding site for the IL 6 inducible nuclear protein IL 6 RE BP , which also binds to the conserved IL 6 REs of other APP genes and is distinct from C / EBP beta . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
C / EBP beta ( LAP ) , another liver enriched basic leucine zipper transcription factor , bound to these same sites but effected a more modest increase in CYP 7 directed gene transcription ( up to 3 4 fold ) when expressed in HepG 2 cells . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
To better understand the regulation of this process we have studied the expression of liver enriched transcriptional factors ( HNF 1 alpha and HNF 1 beta , HNF 3 alpha , HNF 3 beta , and HNF 3 gamma , HNF 4 , C / EBP , C / EBP beta , and DBP ) in an experimental model of oval cell proliferation and differentiation and compared the expression of these factors to that observed during late stages of hepatic ontogenesis . ^^^ The steady state mRNA levels of four ( HNF 1 alpha , HNF 3 alpha , HNF 4 , and C / EBP beta ) `` liver enriched ' ' transcriptional factors gradually decrease during the late period of embryonic liver development while three factors ( HNF 1 beta , HNF 3 beta , and DBP ) increase . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
LAP ( Liver Activator Protein ) is a member of the C / EBP family , which in differentiated hepatocytes contributes to the high levels of liver specific gene expression . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Other transcription factors , such as GAL 4 VP16 , GAL 4 , c Jun or C / EBP beta are also stimulated by the cotransfected C terminus . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Activation of this cell line results in altered expression of several nuclear DNA binding complexes involving two members of the C / enhancer binding protein ( EBP ) family of transcription factors , namely C / EBP beta ( nuclear factor ( NF ) IL 6 / LAP ) and Ig / EBP 1 ( C / EBP gamma ) . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
A mutation in the cAMP response element ( CRE ) ( 87 to 74 ) , which binds CCATT / enhancer binding protein beta ( C / EBP beta ) and / or cAMP response element binding protein ( CREB ) , resulted in a 4 and 20 fold elevation in the level of bGH mRNA in the liver and kidney of transgenic mice , respectively . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Promoter P 1 is stimulated by the liver enriched transcription factors C / EBP and LAP , whereas in the proximal region of P 3 we have identified several elements that are recognized by transcription factors , including krox20 / egr2 and krox24 / erg1 . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Serum amyloid A gene expression under acute phase conditions involves participation of inducible C / EBP beta and C / EBP delta and their activation by phosphorylation . ^^^ In this study , using antibodies against three C / EBP isoforms in DNA binding and Western blot ( immunoblot ) assays , we found that in response to inflammatory signals , both C / EBP delta and C / EBP beta are induced and that their interactions with the SAA promoter element are necessary for the increased SAA gene expression . ^^^ Cotransfections of liver cells with an SAA promoter linked reporter chloramphenicol acetyltransferase gene and murine sarcoma virus expressed C / EBP delta or C / EBP beta confirm such phenomena . ^^^ These results suggest that C / EBP delta is regulated by phosphorylation and , in conjunction with C / EBP beta , is one of the major proteins responsible for the increased transcription of the SAA gene in response to inflammatory stimuli . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
LAP ( NF IL 6 or C / EBP beta ) , is a liver transcriptional activator protein that confers liver specific gene expression . ^^^ LAP ( NF IL 6 or C / EBP beta ) , is a liver transcriptional activator protein that confers liver specific gene expression . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Anti C / EBP beta ( NFIL 6 ) and anti C / EBP delta ( NFIL 6 beta ) Abs identified both of these proteins in complexes formed between the NFIL 6 like element and mononuclear cell nuclear extracts . ^^^ These data are consistent with the requirement for C / EBP beta ( NFIL 6 ) and C / EBP delta ( NFIL 6 beta ) in the activation of murine IL 1 beta gene expression by endotoxin . . ^^^ C / EBP delta ( NFIL 6 beta ) was not detected in complexes utilizing extracts from the IL 1 nonproducing T cell line . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Both HNF 4 and C / EBP beta are required for liver specific activity of the ornithine transcarbamylase enhancer . ^^^ Furthermore , cotransfection experiments showed that both HNF 4 and C / EBP beta are necessary , and neither alone sufficient , for activation of the reconstituted enhancer in nonhepatic cells . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The specific regulatory effects of glucocorticoid receptor , C / EBP beta , and Ets proteins were documented by co transfection . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Further analysis of the 5 ' flanking region revealed a number of putative regulatory elements , such as four interleukin 6 response elements ( IL 6 RE ) , two potential binding sites for NF IL 6 , a TGF beta inhibitory element ( TIE ) , a cAMP response element ( CRE ) , two estrogen response elements ( ERE ) including one located in the first intron , a potential vitamin D response element ( VDRE ) which overlaps a chicken ovalbumin upstream promoter ( COUP ) transcription factor binding element , two liver specific transcription factor ( C / EBP ) binding sites , and a B cell and macrophage specific transcriptional factor binding site ( PU . 1 / ETS ) . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Modulation of the human interleukin 6 promoter ( IL 6 ) and transcription factor C / EBP beta ( NF IL 6 ) activity by p 53 species . ^^^ The functional DNA target for transcriptional modulation of the IL 6 promoter by p 53 species included the multiple cytokine and second messenger response element ( 173 to 145 ) ; point mutations in the transcription factor C / EBP beta binding site within the second messenger response element largely blocked the ability of p 53 mutants Val 135 and Phe 132 to up regulate this promoter . ^^^ The up regulation of IL 6 promoter constructs by co transfection into HeLa cells of a C / EBP beta constitutive expression vector was blocked in a dominant negative manner by wt p 53 . ^^^ In contrast , the p 53 mutants Val 135 and Phe 132 further enhanced C / EBP beta mediated up regulation of IL 6 promoter constructs . ^^^ The modulation of C / EBP beta function by p 53 species provides a basis for the involvement of p 53 not only in the regulation of cytokine synthesis but also in the altered responsiveness of tumor cells to cytokines . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
One of the members of the bZIP family of transcriptional activators is NF IL6 / LAP ( IL 6 DBP , C / EBP beta , CRP 2 ) . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The gene encoding the liver enriched transcriptional activator protein LAP ( C / EBP beta ) has also been shown to issue two proteins , LAP and the liver enriched transcriptional inhibitory protein LIP , with different transcription activation potentials . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
We show that IL 6 activates JRE IL 6 through an H 7 sensitive pathway that does not involve protein kinase C , cyclic AMP dependent kinase , Ca ( 2+ ) or calmodulin dependent kinases , Ras , Raf 1 , or NF IL 6 ( C / EBP beta ) . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Evidence for the role of a cis acting C / EBP beta promoter element . ^^^ The subregion 142 / 120 acting element C / EBP beta , 5 ' TTATGCAAT 3 ' . ^^^ Point mutations within the C / EBP beta element abolished protein / DNA binding and resulted in a 50 % loss of forskolin / LH / FSH inducibility of reporter gene activity . ^^^ C / EBP beta mRNA and protein were induced rapidly in granulosa cells in vivo by an ovulatory dose of human chorionic gonadotropin ( hCG ) . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The related C / EBP beta and C / EBP delta proteins are not detectable by sensitive immunocytochemical methods in epithelial cells distributed along the duodenal to colonic axis . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The NF M transcription factor is related to C / EBP beta and plays a role in signal transduction , differentiation and leukemogenesis of avian myelomonocytic cells . ^^^ Here we describe the cloning of NF M , a myeloid specific transcription factor related to C / EBP beta , which is a target of activated protein kinases . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
We have cloned a chicken C / EBP related gene that is highly expressed in myeloid cells and identified it as the chicken homolog of C / EBP beta . ^^^ A dominant negative variant of chicken C / EBP beta interferes with the 5 myb induced activation of the mim 1 gene in these cells , suggesting that C / EBP beta or another C / EBP transcription factor is required for the activation of mim 1 by 5 myb . ^^^ We found that C / EBP beta and other C / EBP transcription factors confer to fibroblasts the ability to induce the mim 1 gene in the presence of 5 myb . ^^^ Our results suggest a role for C / EBP beta , and possibly for other C / EBP transcription factors , in 5 myb function and in myeloid specific gene activation . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
C / EBP , LAP , DBP , HNF 3 , and vHNF 1 expression are not systematically extinguished in parallel with the hepatic functions . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
We identified the HeLa binding protein by screening a cDNA expression library with the specific promoter site as a probe and demonstrate now that the activating protein is identical to the nuclear factor for interleukin 6 expression ( NF IL 6 ) , a member of the C / EBP family of transcription factors . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
One of the essential proteins which bound to the enhancer was similar or identical to members of the C / EBP family of transcription factors required for both IL 1 and LPS specific induction of the IL 6 gene ( i . e . , the NF IL 6 proteins ) . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
In cotransfection experiments , the C / EBP beta protein trans activated 10 15 fold the cAspAT gene promoter in HepG 2 cells . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NF IL 6 , an IL 6 inducible transcription factor of the C / EBP family , can confer this regulation and is itself regulated by both signals . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Two transcription factors , the CCAAT / enhancer binding protein ( C / EBP ) and the liver activator protein ( LAP ) , were previously shown to bind to the ADH promoter at nucleotide positions 11 to 22 relative to the start site of transcription and to activate the ADH promoter in co transfection experiments . ^^^ In this study , exposure of cultured rat hepatocytes to GH ( 1 micrograms / ml ) for 4 days increased LAP mRNA , but not C / EBP mRNA , in conjunction with an increase in ADH mRNA . ^^^ These results suggest that the effect of GH in enhancing ADH promoter activity is mediated by LAP binding to the C / EBP site . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NF M ( chicken C / EBP beta ) induces eosinophilic differentiation and apoptosis in a hematopoietic progenitor cell line . ^^^ We have shown earlier that NF M , the chicken homolog of C / EBP beta , is specifically expressed in myelomonocytic and eosinophilic cells of the hematopoietic system . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
We report in this study that in these cells , C / EBP beta and C / EBP delta are induced by VIP , PACAP , or NA via the cAMP second messenger pathway . ^^^ Induction of C / EBP beta and delta mRNA by VIP occurs in the presence of a protein synthesis inhibitor . ^^^ Thus , c / ebp beta and c / ebp delta behave as cAMP inducible immediate early genes in astrocytes . ^^^ Moreover , transfection of astrocytes with expression vectors selectively producing the transcriptionally active form of C / EBP beta , termed liver enriched transcriptional activator protein , or C / EBP delta enhance the glycogen resynthesis elicited by NA , whereas an expression vector producing the transcriptionally inactive form of C / EBP beta , termed liver enriched transcriptional inhibitory protein , reduces this resynthesis . ^^^ These results support the idea that C / EBP beta and delta regulate gene expression of energy metabolism related enzymes in astrocytes . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
C / EBP beta mRNA levels increase between post natal days 4 and 8 and remain constant subsequently , in contrast to a decrease in C / EBP beta protein levels between post natal days 11 and 15 . ^^^ C / EBP beta and delta are both predominantly nuclear in crypt and differentiated surface epithelial cells , as well as in various cells of the lamina propria and muscular layers . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Regulation of C / EBP beta / NF M activity by kinase oncogenes . ^^^ One member of this family known as C / EBP beta ( called NF M in the chicken system ) is particularly important in myelomonocytic cells because it is targeted by kinases and collaborates with the Myb oncoprotein to induce the expression of myeloid specific genes . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Mouse chromosomal location of the CCAAT / enhancer binding proteins C / EBP beta ( Cebpb ) , C / EBP delta ( Cebpd ) , and CRP 1 ( Cebpe ) . ^^^ There are four known members of the C / EBP family of basic region leucine zipper transcription factors : Cebpa , Cebpb , Cebpd , and Cebpe . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Several liver and epidermis specific transcription factor binding sites , including C / EBP , NFIL 6 , HNF 5 , AP2 / KER1 , MNF , and others , are also identified in the 5 ' flanking region . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The former included putative NF IL 6 ( C / EBP beta ) and AP 2 elements , and the latter contained the NF kappa B motif . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
During differentiation RB was found to interact directly through its simian virus 40 large tumor antigen ( T antigen ) binding domain with NF IL 6 , a member of the CAAT / enhancer binding protein ( C / EBP ) family of transcription factors . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
In this report we have demonstrated that treatment of rat pheochromocytoma PC 12 cells with sodium arsenite leads to enhanced expression of C / EBP beta and GADD 153 ( growth arrest and DNA damage inducible gene 153 ) but not other C / EBPs . ^^^ Coimmunoprecipitation experiments provided evidence for the formation of endogenous GADD 153 C / EBP beta complexes in arsenite treated cells . ^^^ Here , we demonstrate that extracts prepared from arsenite treated PC 12 cells likewise show increased amounts of factors capable of binding to the GADD 153 C / EBP site and that these complexes are comprised at least in part of C / EBP beta . ^^^ Forced expression of C / EBP beta was found to be capable of transactivating the GADD 153 promoter in PC 12 cells cotransfected with plasmids expressing a GADD 153 reporter gene and C / EBP beta protein . ^^^ However , overexpression of GADD 153 inhibited the transactivation of the GADD 153 promoter by C / EBP beta . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
A computer homology search for a 1 . 2 Kb promoter region showed that there are several consensus cis DNA elements such as C / EBP , NF IL 6 , GRE and AP 1 in this region . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Interestingly , dietary protein restriction specifically increased the level of a truncated form of C / EBP beta ( liver enriched transcriptional inhibitory protein , LIP ) , which is a protein dominant negative inhibitor of C / EBP function . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
A number of cis acting regulatory elements residing immediately 5 ' of the Form 2 initiation site includes AP 1 , AP 2 , NF kappa B , NF IL 6 , C / EBP , and CREB . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
A nuclear factor binding to the element is identified as NF IL 6 ( also termed as LAP and C / EBP beta ) . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
These substrates include transcription factors that are regulated by MAP kinase phosphorylation ( e . g . , Elk 1 , c Myc , c Jun , c Fos , and C / EBP beta ) . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
CCAAT / enhancer binding protein beta ( C / EBP beta ) binds and activates while hepatocyte nuclear factor 4 ( HNF 4 ) does not bind but represses the liver type arginase promoter . ^^^ In addition to area A , a recombinant C / EBP beta protein bound to area B , which appeared to be primarily responsible for activation by C / EBPs . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Western ( immunoblot ) and Southwestern ( DNA protein ) analyses show that p 42 C / EBPalpha forms specific complexes with the alpha 1 acid glycoprotein oligonucleotide in control nuclear extract and that p 20 C / EBP beta forms complexes in LPS treated liver . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Cultures infected with AdCEBPalpha had 50 fold higher levels of C / EBPalpha mRNA and protein than those infected with Ad beta gal , but similar expression of C / EBP beta . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Recent studies have found that several different liver enriched transcription factors including HNF 1 alpha , HNF 3 , HNF 4 , and C / EBP beta , and the more ubiquitously expressed factors Sp 1 , GABP alpha / beta , and NF2d9 are responsible for governing the transcription of P 450 genes . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Transcriptional induction of the alpha 1 acid glycoprotein ( AGP ) gene by synergistic interaction of two alternative activator forms of AGP / enhancer binding protein ( C / EBP beta ) and NF kappaB or Nopp 140 . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
U 1 lines overexpressing C / EBP activator NF IL 6 produced more viral mRNA and virus particles following cellular activation than control lines , demonstrating that C / EBP proteins are limiting for virus transcription . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Nuclear factor kappa B ( NF kappa B ) , nuclear factor interleukin 6 ( NFIL 6 or C / EBP beta ) and nuclear factor interleukin 6 beta ( NFIL 6 beta or C / EBP delta ) are not sufficient to activate the endogenous interleukin 6 gene in the human breast carcinoma cell line MCF 7 . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Deletion and mutational analysis of the promoter highlighted the role of the 185 / 60 region and showed that in both MCF 7 and HeLa cells , the nuclear factor IL 6 ( NF IL 6 ) site cooperates with the nuclear factor kappa B ( NF kappa B ) motif to produce maximal induction by TNF alpha , whereas the CCAAT / enhancer binding protein ( C / EBP ) site displayed different cooperative effects toward NF kappa B depending on the cell line used . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The tissue specific transcription of this gene may be due to the presence of binding sites for the hepatocyte nuclear factors LF A 1 , HNF 5 , NF IL 6 , and C / EBP . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Regulation of HIV 1 long terminal repeats by interaction of C / EBP ( NF IL 6 ) and NF kappaB / Rel transcription factors . ^^^ We report the characterization of a CAAT enhancer binding protein ( C / EBP ) ( NF IL 6 ) element encompassing the region from 174 to 166 of the U 3 long terminal repeat ( LTR ) region of HIV 1 . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
In contrast , overexpression of C / EBP beta or C / EBP delta had no effect on hepatoma cell growth . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Induction of the C / EBP beta gene by dexamethasone and glucagon in primary cultured rat hepatocytes . ^^^ The synthetic glucocorticoid , dexamethasone , and glucagon cooperatively elevated the level of mRNA for the transcription factor CCAAT / enhancer binding protein beta ( C / EBP beta ) in primary cultured rat hepatocytes . ^^^ In response to dexamethasone and / or glucagon , C / EBP beta mRNA started to increase as early as 30 min , reached a maximum within 2 h , and then gradually decreased . ^^^ The administration of cycloheximide , a protein synthesis inhibitor , led rather to an increase in C / EBP beta mRNA , which suggested that a labile negative protein factor ( s ) is involved in regulation of the C / EBP beta mRNA level . ^^^ Cycloheximide further augmented the increases in C / EBP beta mRNA by dexamethasone and / or glucagon . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Another factor SF B ( silencer factor B ) has been cloned and found to be the same as LIP ( liver inhibitory protein ) which is a competitor for LAP ( liver activator protein ) , both are from the same gene designated as C / EBP beta . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Inactivation of the IL 6 gene prevents development of multicentric Castleman ' s disease in C / EBP beta deficient mice . ^^^ We have previously shown that mice lacking the trans acting factor C / EBP beta , a transcriptional regulator of IL 6 and a mediator of IL 6 intracellular signaling , develop a pathology nearly identical to multicentric Castleman ' s disease , together with increasingly high levels of circulating IL 6 . ^^^ We describe here how the simultaneous inactivation of both IL 6 and C / EBP beta genes prevents the development of pathological traits of Castleman ' s disease observed in C / EBP beta deficient mice . ^^^ Histological and phenotypic analysis of lymph nodes and spleen of double mutant mice did not show either the lymphoadenopathy and splenomegaly or the abnormal expansion of myeloid , B and plasma cell compartments observed in C / EBP beta / mice , while B cell development , although delayed , was normal . ^^^ Our data demonstrate that IL 6 is essential for the development of multicentric Castleman ' s disease in C / EBP beta / mice . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
A novel C / EBP beta YY 1 complex controls the cell type specific activity of the human papillomavirus type 18 upstream regulatory region . ^^^ Here , we show that a protein complex composed of C / EBP beta and YY 1 binds the switch region which is detected only in HeLa cells , not in HepG 2 cells . ^^^ Transfection of C / EBP beta into HepG 2 cells restored the formation of the C / EBP beta YY 1 switch region complex , accompanied by increased transcription directed by the HPV 18 URR . ^^^ Mutations in the switch region that abolished the complex formation also abrogated C / EBP beta induced transcriptional activation . ^^^ This provides a strong correlation between the binding of the C / EBP beta YY 1 complex to the switch region and cell type specific URR activity . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Induction of hemopexin , alpha 1 acid glycoprotein , alpha 2 macroglobulin , GADD 153 , C / EBP beta , and C / EBP delta mRNAs by LPS were all abolished in dexamethasone treated rats . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The human interleukin 6 ( IL 6 ) promoter contains two regulatory elements , a kappa B enhancer and a NFIL 6 ( C / EBP beta ) binding site , which have been reported to be essential for inducibility of the IL 6 gene . ^^^ Finally , transfection experiments , in which NF kappa B subunits , NFIL 6 , and NFIL 6 beta ( C / EBP delta ) , were overexpressed in cells transfected with mutated IL 6 enhancer elements linked to a reporter gene show that interaction between members of the two families of factors is required for activation of the IL 6 gene in the absence of the NFIL 6 binding site . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
This induction of PEPCK expression by cAMP occurred in the presence of sustained levels of CCAAT / enhancer binding protein ( C / EBP ) alpha delta mRNAs , and was accompanied by an increase in the rate of transcription and mRNA content of C / EBP beta gene , and a decrease in the expression of c myc , in the absence of c fos expression . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Additionally , we found that Myb / En / ER could counteract transactivation by C / EBP beta of the mim 1 promoter , which contains juxtaposed Myb and C / EBP binding sites . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The 5 ' flanking region of AT 2 R contained several cis regulatory elements , such as AP 1 , AP 2 , C / EBP , NF 1 , NF IL 6 , NF kappa B , and glucocorticoid and cAMP responsive elements ( CRE ) . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
In vivo footprinting with limiting amounts of embryo tissues : a role for C / EBP beta in early hepatic development . ^^^ Comparison of the in vivo protection pattern with that obtained from the in vitro analysis of different C / EBP isoforms suggests that in embryonic hepatocytes , C / EBP beta is bound to the albumin gene enhancer . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
By using a PCR based amplification assay we selected high affinity DNA binding sites for C / EBP beta and the PAR protein DBP from a pool of oligonucleotides . ^^^ Here we have characterized a single amino acid substitution mutant of C / EBP beta that increases its target site specificity . ^^^ This protein , C / EBP beta 5 > A , contains a valine to alanine substitution at position 13 of the basic domain ( residue 216 of C / EBP beta ) . ^^^ C / EBP beta 5 > A selectively binds only the subset of C / EBP sites that are also DBP sites , both as oligonucleotides and within the natural contexts of the albumin and cholesterol hydroxylase promoters . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Coordination of transcription factors , NF Y and C / EBP beta , in the regulation of the mdr1b promoter . ^^^ The expression of CCAAT / enhancer binding protein beta ( C / EBP beta ) in Y 1 cells augmented mdr1b promoter activity and resulted in an increased level of mdr1b mRNA . ^^^ The effect of C / EBP beta expression on mdr1b promoter activity was sensitive to mutations in the Y box , suggesting that coordination of NF Y with C / EBP beta is required for further activation of the mdr1b promoter . ^^^ Our studies have indicated that NF Y is a critical factor for the mdr1b promoter , and its coordination with other factors , such as C / EBP beta , could be an important mechanism involved in mdr1b gene expression . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The 35kD nucleoprotein that displayed an acute phase inducible affinity to bind DRE was identified as a C / EBP beta isoform . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Binding elements for the nuclear transcriptional regulatory factors ( NF ) kappa B and NF IL 6 ( C / EBP beta ) are present in the promoter regions of multiple cytokine genes , including those whose expression is increased after blood loss . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Expression of the alpha 1 acid glycoprotein ( AGP ) gene ( agp ) is activated by a key transcription factor , AGP / enhancer binding protein ( AGP / EBP , commonly called C / EBP beta ) , in the liver during the acute phase response . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NF IL 6 ( or C / EBP beta ) is a multifunctional transcription factor of the C / EBP family . ^^^ NF IL 6 ( or C / EBP beta ) is a multifunctional transcription factor of the C / EBP family . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The mRNA for albumin and D site binding protein ( DBP ) was more abundant and mRNA for CCAAT / enhancer binding protein beta ( C / EBP beta ) was less abundant in the cyclic parenteral nutrition group than in the continuous parenteral nutrition group . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
We demonstrate that the NRE contains a CCAAT box and that both RAR / RXR and CCAAT binding proteins such as c / EBP beta recognize this common regulatory sequence in the hMGP promoter . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Induction by staurosporine of nitric oxide synthase expression in vascular smooth muscle cells : role of NF kappa B , CREB and C / EBP beta . 1 . ^^^ They also indicate that NF kappa B and a member of the C / EBP family of transcription factors , presumably C / EBP beta , act synergistically under basal conditions and possibly also following exposure to IL 1 beta in the up regulation of iNOS gene expression in these cells . ^^^ Targeting of the activation of C / EBP beta may thus represent an interesting approach to interfere selectively with the cytokine induced over production of NO in acute and chronic inflammatory conditions . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Transcriptional induction of the agp / ebp ( c / ebp beta ) gene by hepatocyte growth factor . ^^^ In this report , we present evidence that shows HGF can stimulate the expression of AGP / EBP ( C / EBP beta ) and NF kappaB , which are both key transcription factors responsible for the regulation of many genes under stress conditions or during the acute phase response . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The flanking region of the BST 1 gene contained several potential binding sites for nuclear factors , NF kappa B , p 53 , NF IL 6 , CREB , PEA 3 , E2A , C / EBP , AP 3 , AP 2 and SP 1 and consensus sequences for gamma IRE and ISRE like element . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
In humans , similar effects were observed with homologous members of the CCAAT / enhancer binding protein ( C / EBP ) family of transcriptional regulators , especially the human homolog of chicken NF M , C / EBP beta ( NF IL 6 ) . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
C / EBP beta is induced during G 1 of the cell cycle . ^^^ In contrast to COMMA D cells , C / EBP beta and C / EBP delta mRNA levels remain relatively constant in growth arrested and cell cycle induced NIH3T3 cells . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Analysis of the 5 ' flanking sequence of HPRT 2 revealed regions with homology to the liver specific regulatory motifs C / EBP , NF IL 6 , LF A 1 and LF B 1 , although the functional significance of these regions remains unknown . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Rapid communication : nucleotide sequence of bovine C / EBP beta gene . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The NF kappa B and NF IL 6 DNA binding proteins were immunochemically characterized as a heterodimer p65 / p50 and a homodimer C / EBP beta , respectively . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Increased CRH promoter activity following phorbol ester treatment was inhibited by a dominant negative NF IL 6 mutant , showing that the effects of phorbol ester were principally mediated by C / EBP . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The mutation occurs at a DNA binding site for the ubiquitous transcription factor octamer 1 ( Oct 1 ) and results in a loss of cooperativity between Oct 1 and the tissue specific transcription factor , NF IL 6 ( C / EBPbeta ) , a member of the C / EBP family of transcription factors . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
We show that the +KTS variant effectively represses promoter activity under all conditions tested but the KTS variant was only able to repress in the presence of cotransfected C / EBP beta or a dominant negative p 53 mutation . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The promoter region of the mouse gene contains various cis acting elements , including a TATA like box and a GC box as well as potential binding sites for the transcription factors NF IL 6 , MyoD , GATA , C / EBP , AP 2 , NF kappaB , AP 1 , and Sp 1 . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The transcription factor C / EBP beta ( CCAAT / enhancer binding protein beta ) is expressed in ovaries and testes , as well as many other tissues of adult mice . ^^^ Here we show that mice carrying a targeted deletion of the C / EBP beta gene exhibit reproductive defects . ^^^ In normal ovaries , C / EBP beta mRNA is specifically induced by luteinizing hormone ( LH / hCG ) in the granulosa layer of preovulatory antral follicles . ^^^ C / EBP beta deficient ovaries lack corpora lutea and fail to down regulate expression of the prostaglandin endoperoxidase synthase 2 and P 450 aromatase genes in response to gonadotropins . ^^^ These findings demonstrate that C / EBP beta is essential for periovulatory granulosa cell differentiation in response to LH . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Tumor necrosis factor alpha and interleukin 1beta regulate the murine manganese superoxide dismutase gene through a complex intronic enhancer involving C / EBP beta and NF kappaB . ^^^ The 5 ' portion of the TNFRE bound C / EBP beta in vitro and was both necessary and sufficient for TNF responsiveness with the MnSOD promoter or with a heterologous promoter when in an upstream position . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Analyses of specific nucleotide substitutions further indicated that the Tax induced transcriptional activation requires two transcription factor binding motifs within the IL1B promoter ; one is a binding site for nuclear factor ( NF ) IL 6 ( CCAAT / enhancer binding protein beta , C / EBP beta ) , which belongs to the basic region leucine zipper ( bZIP ) family and the other for Spi 1 ( PU . 1 ) , which is an Ets family protein found principally in monocytes , macrophages , and B lymphocytes . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Furthermore , we have delineated the mechanism of the transcriptional activation of Ax 1 by PMA and the involvement of a specific transcription factor , nuclear factor interleukin 6 ( C / EBP beta ) . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Here we show directly that the elevation of IL 6 in vivo either in mice transgenic for the IL 6 gene or in knock out mice lacking a functional gene for the transcription factor C / EBP beta ( NF IL 6 ) does indeed result in elevated hsp 90 levels . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
This region of the gene also contains consensus sequences for liver enriched transcription factors , C / EBP beta and HNF 4 , as well as for the ubiquitous protein factors , AP 1 , H4TF1 , NF 1 , and Sp 1 . ^^^ Competitive gel shift and supershift assays with different synthetic oligonucleotide probes demonstrate that proteins contained in the nuclear extract , identified as C / EBP beta , H4TF1 , and HNF 4 , bind to a region of the murine fVII DNA from 85 to 32 bp upstream of the transcription start site . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The transcriptional activation pattern of lipopolysaccharide binding protein ( LBP ) involving transcription factors AP 1 and C / EBP beta . ^^^ Our results furthermore show that AP 1 and C / EBP beta are transcription factors involved in the activation of the LBP gene , as revealed by Luciferase reporter gene analysis and electromobility shift assays . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
IL 6 signalling is known to involve the activation of two independent transcription factors : Stat 3 ( through phosphorylation by Jak kinases ) and C / EBP beta ( through activation of the ras pathway ) . ^^^ In addition , C / EBP beta is believed to act as a transcriptional activator of the IL 6 gene itself . ^^^ On the other hand , making use of C / EBP beta deficient mice , we show that the induction of IL 6 by a variety of stimuli does not require C / EBP beta activity . ^^^ In contrast to the predicted activating role of C / EBP beta , IL 6 levels are increased in the C / EBP beta deficient mice , suggesting that C / EBP beta may act as a down modulator of the IL 6 gene . ^^^ Through the generation of C / EBP beta , IL 6 double mutant mice we show that IL 6 hyperproduction is responsible for the development of the Castleman ' s like lymphoproliferative disease described in the C / EBP beta deficient mice , since the disorder is completely blocked by inactivating the IL 6 gene . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Currently , we investigate whether NF M , a C / EBP beta family member , cooperates with c Myb in activating topo 2 alpha transcription . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
During normal mammary gland development , expression of LIP ( liver enriched inhibitory protein , a dominant negative isoform of C / EBP beta ) is hormonally regulated and correlates with cell proliferation during pregnancy . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
C / EBP beta , C / EBP delta and ADD 1 / SREBP 1 are active early during the differentiation process and induce the expression and / or activity of the peroxisome proliferator activated receptor gamma ( PPAR gamma ) , the pivotal coordinator of the adipocyte differentiation process . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Synergistic activation of the germline epsilon promoter mediated by Stat 6 and C / EBP beta . ^^^ Here , we show that Stat 6 and C / EBP beta cooperate to synergistically activate transcription from the epsilon element . ^^^ Stat 6 and C / EBP beta bind the IL 4 response element simultaneously . ^^^ The fast dissociation rate apparent when Stat 6 binds this DNA element alone is slowed when C / EBP beta binds at the neighboring site . ^^^ These data suggest a mechanism whereby C / EBP beta stabilizes Stat 6 binding at this element , thereby increasing the likelihood that both of their activation domains will interact , possibly with other factors , to activate transcription in an IL 4 dependent manner . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The 35kD nucleoprotein that displayed an acute phase inducible affinity to bind hormone REs of rat Hp and alpha 2MG genes , was identified as a C / EBP beta isoform . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NF IL 6 is an important transcriptional regulator of genes induced in activated monocytes / macrophages , and NF IL 6 is the only CCAAT / enhancer binding protein ( C / EBP ) family member whose steady state mRNA levels increase upon activation of monocytes ( 1 ) . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
DNA protein binding studies by competition in electromobility shift and supershift assays revealed that 2 members , C / EBP beta and C / EBP delta , are mainly responsible for DNA protein complex formation ; the former acts as a transcriptional repressor and the latter as an activator of the PDGF alphaR gene , respectively . ^^^ Furthermore , forced expression of C / EBP delta transactivated the transcriptional efficiency of the PDGF alphaR gene even in WKY derived VSMCs , whereas that of C / EBP beta had an opposite effect in SHR derived VSMCs . ^^^ These findings indicate that differential expression of members of the C / EBP family , mainly C / EBP delta and possibly C / EBP beta , are responsible for the strain specific gene transcription of PDGF alphaR in VSMCs . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
In contrast , mRNA levels of C / EBP beta were highest in the PN group and lowest in the OR group . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Treatment with FSH or 8 CPTcAMP strongly induced C / EBP beta mRNA above basal levels with rapid and transient kinetics in Sertoli cell primary cultures as well as in whole testes from hypophysectomized rats . ^^^ Whereas C / EBP beta mRNA was induced approximately 50 fold , C / EBP delta mRNA was induced 5 to 8 fold by cAMP in Sertoli cells . ^^^ Messenger RNA for C / EBP beta and delta were induced by inhibition of protein synthesis with cycloheximide and cycloheximide acted synergistically with cAMP . ^^^ Immunoblots with C / EBP antibodies demonstrated a strong induction of C / EBP beta , delta , and zeta by cAMP . ^^^ Electrophoretic mobility shift analysis of nuclear proteins from cAMP treated Sertoli cells using a C / EBP consensus oligonucleotide and antibodies revealed specific binding of C / EBP / DNA complexes , the majority of which were supershifted by C / EBP beta antibody . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
In contrast , the C / EBP beta mRNA level showed a significant increase at day 1 . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Among the other c / ebp family members , only c / ebp beta was also upregulated during erythroid differentiation . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
SF 1 ( steroidogenic factor 1 ) and C / EBP beta ( CCAAT / enhancer binding protein beta ) cooperate to regulate the murine StAR ( steroidogenic acute regulatory ) promoter . ^^^ Characterization of these sites by EMSA indicated that C / EBP beta bound with high affinity to C 1 and C 2 was a low affinity C / EBP site . ^^^ These data indicate that in addition to SF 1 , C / EBP beta is involved in the transcriptional regulation of the StAR gene and may play an important role in developmental and hormone responsive regulation of steroidogenesis . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Western blot analysis of hepatocytes taken from various gestation stages of rat liver showed that the expression of RB and C / EBP beta increased as gestation stage proceeded . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
C / EBP beta in rheumatoid arthritis : correlation with inflammation , not disease specificity . ^^^ Rheumatoid arthritis synovial tissue was examined and compared with osteoarthritis tissue for the presence of the nuclear transcription factor C / EBP beta ( NF IL 6 ) . ^^^ The region ( lining or sublining ) , cell type , and subcellular distribution ( cytoplasmic or nuclear ) of the expression of C / EBP beta was characterized . ^^^ C / EBP beta was detected in the synovial lining and in sublining cells of synovial tissue from patients with both rheumatoid and osteoarthritis . ^^^ In both diseases a strong correlation ( r = 0 . 79 , P < 0 . 001 ) was observed between the percentage of cells in the synovial lining that were positive for nuclear C / EBP beta and lining cell depth . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
This study investigated the relation between tissue nuclear factor kappaB ( NFkappaB ) and nuclear factor interleukin 6 ( NF IL 6 or C / EBP ) activation and bacteremia , inflammatory cytokine expression , and mortality in a murine model of cecal ligation and puncture ( CLP ) . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
C / EBP beta , a leucine zipper transcription factor , acts in an IL 6 independent fashion to induce a separate set of genes and proteins and is also required for normal liver regeneration . ^^^ Moreover , some early growth response genes such as PRL 1 , which encodes a nuclear protein tyrosine phosphatase , are induced normally in the absence of C / EBP beta and IL 6 and highlight the role of other transcriptional complexes such as Egr 1 in the early phases of liver regeneration . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Consistent with this concept , the present study identifies several phenotypic abnormalities in macrophages from ob / ob mice , including decreased steady state levels of uncoupling protein 2 mRNA , increased mitochondrial production of superoxide and hydrogen peroxide , constitutive activation of CCAAT enhancer binding protein ( C / EBP ) beta , an oxidant sensitive transcription factor , increased expression of interleukin 6 and cyclooxygenase ( COX ) 2 , two C / EBP beta target genes , and increased COX 2 dependent production of PGE 2 . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NF IL 6 belongs to the CCAAT / enhancer binding protein ( C / EBP ) of transcription factors and plays a crucial role in activated macrophages . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The EMSAs indicated that sodium butyrate suppressed TNF alpha induced nuclear factor ( NF ) kappaB and activation protein ( AP ) 1 DNA binding activity , but enhanced TNF alpha induced activation of CCAAT / enhancer binding protein ( C / EBP ) beta ( NF IL 6 ) DNA binding activity . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Transient transfection studies using reporter constructs containing either wild type COX 2 regulatory sequences or mutated cis acting sequences linked to a luciferase reporter gene identify a CRE site and two NF IL 6 ( C / EBP ) sites which play important roles in the regulation of COX 2 expression in response to all these agents in osteoblasts . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
In glial cells , full activation needs the presence of the C / EBP binding sites ; however , NF IL 6 is still able to function via the minimal 40 / +80 region . ^^^ In microglial cells , C / EBP sites are not essential , since NF IL 6 acts through the 68 / +80 LTR region , containing two binding sites for the transcription factor Sp 1 . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
A C / EBP beta isoform recruits the SWI / SNF complex to activate myeloid genes . ^^^ Although C / EBP beta is known to cooperate with Myb to induce transcription of the granulocyte specific mim 1 gene , the molecular mechanism of this cooperativity is undefined . ^^^ We show that the N terminus of the full length C / EBP beta isoform , which is essential for induction of the mim 1 gene in chromatin , interacts specifically with the SWI / SNF complex . ^^^ Grafting this domain onto Myb generates a chimeric activator that recruits SWI / SNF and induces mim 1 transcription in the absence of C / EBP beta . ^^^ Interaction between C / EBP beta and SWI / SNF is essential for activating a subgroup of resident target genes in chromatin and may represent a major determinant of combinatorial gene regulation in eukaryotes . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
However , C / EBP beta protein levels did not change regardless of insulin deprivation . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Local signals induce CCAAT / enhancer binding protein delta ( C / EBP delta ) and C / EBP beta mRNA expression in the involuting mouse mammary gland . ^^^ Our laboratory and others have shown that C / EBP delta and C / EBP beta mRNA expression is closely associated with normal mouse mammary gland involution . ^^^ C / EBP beta mRNA expression is also relatively low in nonsealed glands , but is induced in sealed glands within 72 h . ^^^ These data demonstrate that local factors are sufficient to induce C / EBP beta and C / EBP delta in the mouse mammary gland . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Transcription factor , NF IL 6 recognizes the same nucleotide sequences as C / EBP , and it is predominantly expressed in macrophages . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Using the native rnCGM 3 promoter construct , we demonstrated that the promoter activity stimulated by C / EBP beta was also repressed by rRBPJ kappa 2N but enhanced by NotchIC . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Two major DNA binding proteins exhibiting different types of protein DNA interactions were detected : a DNA sequence specific 32 kDa isoform of transcription factor C / EBP beta , and a nuclear matrix protein p 55 that bound to the DNA nonspecifically . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Two other transcription factors , C / EBP beta and NF kappa B , have been implicated in the transcription of the COX 2 gene . ^^^ However , PD 98059 and / or SB 203580 did not prevent the LPS induced increase in the level of the transcription factor C / EBP beta , and none of the four inhibitors used in this study prevented the activation of NF kappa B . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Protein expression and constitutive phosphorylation of hematopoietic transcription factors PU . 1 and C / EBP beta in acute myeloid leukemia blasts . ^^^ We examined the expression and phosphorylation status of hematopoietic transcription factors PU . 1 and C / EBP beta detected by the retarded mobility of the phosphorylated forms of the proteins . ^^^ The expression of C / EBP beta and PU . 1 were detectable in 77 % and 61 % , respectively , of 90 AML samples examined . ^^^ The expressed PU . 1 and C / EBP beta was always accompanied with both phosphorylated and unphosphorylated forms of PU . 1 and C / EBP beta , respectively . ^^^ PU . 1 and C / EBP beta were simultaneously detected in all M 0 , M 4 , M 5 and peripheral blood monocytes , whereas in M 2 and M 3 , the expression of the 2 transcription factors varied among samples . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Phosphorylation of rat serine 105 or mouse threonine 217 in C / EBP beta is required for hepatocyte proliferation induced by TGF alpha . ^^^ We report that TGF alpha induces activation of the p 90 ribosomal S kinase ( RSK ) , which results in the phosphorylation of rat C / EBP beta on Ser 105 and of mouse C / EBP beta on Thr 217 and concomitantly stimulates proliferation in differentiated hepatocytes . ^^^ Moreover , C / EBP beta / mouse hepatocytes respond to TGF alpha when wild type C / EBP beta is reexpressed , whereas they remain refractory to the growth effect of TGF alpha when expressing phosphoacceptor mutants rat C / EBP beta Ala 105 or mouse C / EBP beta Ala 217 . ^^^ In contrast , C / EBP beta / hepatocytes expressing the phosphorylation mimic mutants , rat C / EBP beta Asp 105 or mouse C / EBP beta Glu 217 , exhibited marked proliferation in the absence of TGF alpha . ^^^ Thus , a site specific phosphorylation of the transcription factor C / EBP beta is critical for hepatocyte proliferation induced by TGF alpha and other stimuli that activate RSK . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Prolactin enhances CCAAT enhancer binding protein beta ( C / EBP beta ) and peroxisome proliferator activated receptor gamma ( PPAR gamma ) messenger RNA expression and stimulates adipogenic conversion of NIH 3T3 cells . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
TNF alpha gene expression in macrophages : regulation by NF kappa B is independent of c Jun or C / EBP beta . ^^^ A 120 bp TNF alpha promoter reporter , possessing binding sites for NF kappa B ( kappa B 3 ) , C / EBP beta ( CCAAT / enhancer binding protein beta ) , and c Jun , was activated by cotransfection of plasmids expressing the wild type version of each of these transcription factors . ^^^ Employing adenoviral vectors , dominant negative versions of NF kappa B p 65 , and c Jun , but not C / EBP beta , suppressed ( p < 0 . 05 0 . 001 ) LPS induced TNF alpha secretion in primary human macrophages . ^^^ In contrast , no synergy was observed between NF kappa B p 65 , with or without NF kappa B p 50 , and c Jun or C / EBP beta , even in the presence of the coactivator p 300 . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Among these is a gene coding for the pleiotropic transcription factor , CCAAT / enhancer binding protein beta ( C / EBP beta ) . ^^^ We report here that the gene for C / EBP beta binds to GATE and induces gene expression . ^^^ A mutant C / EBP beta interferes with the IFN gamma stimulated transcription of the ISGF3gamma ( p 48 ) promoter . ^^^ Interestingly , the expression of C / EBP beta , not the other members of its family , is induced by IFN gamma . ^^^ These studies thus identify a novel role for C / EBP beta in the IFN signaling pathways . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Induction of PPAR gamma gene transcription during the differentiation of preadipocytes into adipocytes in vitro occurs following an initial phase of cell proliferation and requires a direct involvement of C / EBP beta , C / EBP delta , and glucocorticoids . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Western blot analyses reveals that cJun and the C / EBP family member C / EBP beta are physiologically relevant transcription factors whose expression corresponds with mda 7 mRNA expression . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The crystal structure of the heterodimer formed by the basic leucine zipper ( bZIP ) domains of activating transcription factor 4 ( ATF 4 ) and CCAAT box / enhancer binding protein beta ( C / EBP beta ) , from two different bZIP transcription factor families , has been determined and refined to 2 . 6 A . ^^^ Even in the absence of DNA , the basic region of ATF 4 forms a continuous alpha helix , but the basic region of C / EBP beta is disordered . ^^^ Proteolysis , electrophoretic mobility shift assay , circular dichroism , and NMR analyses indicated that ( 1 ) the bZIP domain of ATF 4 is a disordered monomer and forms a homodimer upon binding to the DNA target ; ( 2 ) the bZIP domain of ATF 4 forms a heterodimer with the bZIP domain of C / EBP beta that binds the cAMP response element , but not CCAAT box DNA , with high affinity ; and ( 3 ) the basic region of ATF 4 has a higher alpha helical propensity than that of C / EBP beta . ^^^ These results suggest that the degree of ordering of the basic region and the fork and the dimerization properties of the leucine zipper combine to distinguish the structurally similar bZIP domains of ATF 4 and C / EBP beta with respect to DNA target sequence . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Fifty percent of the mice homozygous for a deletion in the gene for CCAAT / enhancer binding protein beta ( C / EBP beta / mice ; B phenotype ) die within 1 to 2 h after birth of hypoglycemia . ^^^ Adult C / EBP beta / mice ( A phenotype ) also had difficulty in maintaining blood glucose levels during starvation . ^^^ Agonists ( glucagon , epinephrine , and isoproterenol ) and other effectors of activation of adenylyl cyclase were the same in liver membranes isolated from C / EBP beta / mice and littermates . ^^^ There was a 79 % increase in the concentration of RI alpha and 27 % increase in RII alpha in the particulate fraction of the livers of C / EBP beta / mice relative to wild type mice , with no change in the catalytic subunit ( C alpha ) . ^^^ Thus , a 45 % increase in hepatic cAMP ( relative to the wild type ) would be required in C / EBP beta / mice to activate protein kinase A by 50 % . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
OBJECTIVE : To elucidate the role of two transcription factors , c myb and liver activator protein ( LAP , a member of the C / EBP family ) in the expression of alpha 1 ( 1 ) collagen gene in activated hepatic stellate cells ( HSCs ) . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
StAR gene expression is regulated primarily at the transcriptional level , and the roles of transcription factors that govern basal and cAMP dependent StAR expression including SF 1 , C / EBP beta , Sp 1 and GATA 4 are reviewed . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Protein kinase A dependent stimulation of rat type 2 secreted phospholipase A ( 2 ) gene transcription involves C / EBP beta and delta in vascular smooth muscle cells . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Regulation of IL 6 and IL 8 expression in rheumatoid arthritis synovial fibroblasts : the dominant role for NF kappa B but not C / EBP beta or c Jun . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
By contrast , C / EBP beta , which trans activates the human GL epsilon promoter , inhibits IL 4 induction of the mouse promoter , probably by attenuating the synergistic interaction between AP 1 and Stat 6 . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Consistent with transfection studies , gel mobility shift assays showed that TCDD led to an enhanced DNA binding activity of C / EBP beta transcription factor . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
C / EBP beta and Elk 1 synergistically transactivate the c fos serum response element . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
These data demonstrate that PPAR alpha agonists repress human fibrinogen gene expression by interference with the C / EBP beta pathway through titration of the coactivator GRIP1 / TIF2 . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Both techniques unequivocally demonstrated that activation of these transcription factors , namely that of C / EBP beta , contribute to the increase of ET ( B ) R gene expression in response to cyclic stretch . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
CCAAT enhancer binding protein beta ( C / EBP beta ) activates CCR 5 promoter : increased C / EBP beta and CCR 5 in T lymphocytes from HIV 1 infected individuals . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The transcription factors , NF kappa B and NF IL 6 ( C / EBP beta ) , which coordinately help regulate IL 6 expression , were activated following wounding and preceded the appearance of IL 6 . ^^^ Addition of IL 1 alpha to NHEK cultures increased IL 6 production and activated NF kappa B and C / EBP beta . ^^^ Taken together , these data indicate that constitutive keratinocyte derived IL 1 alpha is a stimulus for IL 6 production in wounded epidermis , the response involves NF kappa B and C / EBP beta transcription factors , and IL 6 may be associated with modulation of keratinocyte differentiation rather than proliferation . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
These included HNF 3 beta , HFH 1 , HFH 2 , HFH 3 , C / EBP , and C / EBP beta , all of which are consistent with the tissue specific expression profiles of the gene . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The induction of cyclooxygenase 2 mRNA in macrophages is biphasic and requires both CCAAT enhancer binding protein beta ( C / EBP beta ) and C / EBP delta transcription factors . ^^^ Here , we make use of the murine macrophage cells RAW 264 as well as of immortalized macrophages derived from mice deficient for the transcription factor CCAAT enhancer binding protein beta ( C / EBP beta ) to explore the molecular mechanisms regulating COX 2 induction in activated macrophages . ^^^ The initial phase is independent of de novo protein synthesis , correlates with cAMP response element binding protein ( CREB ) activation , is inhibited by treatments that abolish CREB phosphorylation and reduce NF kappa B mediated gene activation , and requires the presence of the transcription factor C / EBP beta . ^^^ On the other hand , C / EBP delta appears to be essential in addition to C / EBP beta to effect the second phase of COX 2 gene transcription , which is important for maintaining the induced state and requires de novo protein synthesis . ^^^ Indeed , both phases of COX 2 induction were defective in C / EBP beta / macrophages . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Furthermore , our studies demonstrate that cres mRNA levels are dramatically reduced in the epididymides of C / EBP beta deficient mice . ^^^ These data suggest that the C / EBP family of transcription factors , in particular C / EBP beta , plays a role in the regulation of cres gene expression . ^^^ In support of this finding , Northern blot analysis showed that C / EBP beta is the predominant C / EBP family member expressed in the L beta T 2 gonadotroph cell line and the proximal caput epididymidis . ^^^ Also , gel shift and supershift assays demonstrated that C / EBP beta protein in nuclear extracts from L beta T 2 gonadotroph cells and epididymal cells bound to the two C / EBP sites in the cres promoter . ^^^ Taken together , these studies demonstrate that the C / EBP beta transcription factor is necessary for high levels of cres gene expression in the proximal caput epididymidis and anterior pituitary gonadotroph cells . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The approximately 2000 kb sequenced of the 5 ' flanking region contains multiple potential transcription factor binding sites , including sites for AP 1 , C / EBP beta , GATA , c Rel , ER , ROR alpha , SREBP , and CREB . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
We show here that one of the C / EBP site at 235 promoter region binds to NF IL 6 protein with high affinity and interacts with NF IL 6 and NF IL6beta ( C / EBPdelta ) in Jurkat T cells . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Japanese flounder C / EBP beta was found to be 1561 bp in length ( ORF of 1041 bp ) , encoding for 347 amino acids and a calculated molecular weight of 39 kDa . ^^^ C / EBP beta was detected by Northern blot analysis in the head and posterior kidney , spleen , intestine , liver , brain , heart , gill and testis and further found by RT PCR to be detected in mucus , peritoneal cavity fluid , peripheral blood leucocytes and eye cDNA . ^^^ Phylogenetic analysis placed the Japanese flounder C / EBP beta within the same cluster as previously reported C / EBP beta sequences . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Proteasome inhibitors activate the transcription factors C / EBP beta and delta in human intestinal epithelial cells . ^^^ We treated cultured Caco 2 cells , a human intestinal epithelial cell line , with one of the proteasome inhibitors , MG 132 or lactacystin , and measured C / EBP beta and delta DNA binding activity by electrophoretic mobility shift assay and supershift analysis . ^^^ In addition , nuclear levels of C / EBP beta and delta protein were determined by Western blot analysis . ^^^ Treatment also resulted in increased DNA binding activity and nuclear protein levels of C / EBP beta and delta . ^^^ The results suggest that proteasome inhibition activates the transcription factors C / EBP beta and delta in human intestinal epithelial cells and that this response , at least in part , is caused by induction of the heat shock response . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Expression of dominant negative forms of CREB dramatically reduced the LIF and prostacyclin stimulated C / EBP beta and C / EBP delta expression . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
However , all three mutants , Myb Delta N 20 , Myb Delta N 47 and Myb Delta N 71 cooperated with C / EBP beta in reporter assays . ^^^ In contrast to Myb Delta N 20 and Myb Delta N 47 , however , the Myb Delta N 71 mutant failed to activate the chromatin embedded endogenous mim 1 gene together with C / EBP beta . ^^^ This suggests that an N terminal region ( amino acids 47 71 ) within repeat 1 ( R 1 ) of the murine c Myb DNA binding domain affects activation of chromosomal target genes in collaboration with C / EBP beta . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
In agreement with that , the wild type 5 Myb induces high expression of myeloid factors C / EBP beta , PU . 1 , and Egr 1 in its target cells , whereas SCL , GATA 1 , and c Myb are more abundant in cells expressing the 5 Myb LZR mutant . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Because of its ability to bind to specific C / EBP elements in the promoters of multiple genes and its ability to interact with other transcription factors , NF IL 6 is involved in transcriptional regulation of a wide range of genes . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
A CCAAT / enhancer binding protein ( C / EBP ) binding site located at +22 to +30 was bound by C / EBP beta in a TPA dependent manner and was solely responsible for the TPA responsiveness . ^^^ This increase in C / EBP beta activity occurs through transcriptional and posttranslational regulation , and the latter is mediated by activation of p 90 ribosomal S 6 kinase ( RSK ) : co expression of dominant negative RSK abolished the TPA responsive and C / EBP beta dependent transactivation . ^^^ Also , TPA responsive activation of GAL 4 C / EBP beta chimera required the Ser residue known as the RSK target . ^^^ In SK N MC cells , which display constitutive , high expression of IGF 1 on use of the major promoter , a large amount of C / EBP beta binding was observed with the C / EBP site in the basal state . ^^^ Treatment with PKC inhibitors substantially reduced the promoter activity and mRNA amounts of IGF 1 , with the binding of C / EBP beta to the C / EBP site also being reduced . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Mechanism of c Myb C / EBP beta cooperation from separated sites on a promoter . c Myb , but not avian myeloblastosis virus ( AMV ) 5 Myb , cooperates with C / EBP beta to regulate transcription of myeloid specific genes . ^^^ To assess the structural basis for that difference , we determined the crystal structures of complexes comprised of the c Myb or AMV 5 Myb DNA binding domain ( DBD ) , the C / EBP beta DBD , and a promoter DNA fragment . ^^^ Within the c Myb complex , a DNA bound C / EBP beta interacts with R 2 of c Myb bound to a different DNA fragment ; point mutations in 5 Myb R 2 eliminate such interaction within the 5 Myb complex . ^^^ GST pull down assays , luciferase trans activation assays , and atomic force microscopy confirmed that the interaction of c Myb and C / EBP beta observed in crystal mimics their long range interaction on the promoter , which is accompanied by intervening DNA looping . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
C / EBP beta and delta DNA binding activity was determined by electrophoretic mobility shift assay and supershift analysis . ^^^ In addition , C / EBP beta and delta nuclear protein levels were determined by Western blot analysis . ^^^ Concomitantly , C / EBP beta and delta protein levels were increased in the nuclear fraction of skeletal muscle . ^^^ In additional experiments , we tested the role of glucocorticoids in sepsis induced activation of C / EBP beta and delta by treating rats with the glucocorticoid receptor antagonist RU 38486 . ^^^ This treatment inhibited the sepsis induced activation of C / EBP beta and delta , suggesting that glucocorticoids participate in the upregulation of C / EBP in skeletal muscle during sepsis . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
C / EBP beta mediates iNOS induction by hypoxia in rat pulmonary microvascular smooth muscle cells . ^^^ Exposure of rats to 10 % O ( 2 ) for 4 days caused pulmonary hypertension and induced expression of both inducible nitric oxide synthase ( iNOS ) and CCAAT box enhancer binding protein beta ( C / EBP beta ) in rat lung . ^^^ Electrophoretic mobility shift assays ( EMSAs ) showed that exposure to 1 % O ( 2 ) increased the C / EBP beta binding in rat pulmonary microvascular smooth muscle cells ( rPSMs ) . ^^^ To test the hypothesis that C / EBP beta participates in hypoxia induced iNOS expression in rPSMs , a C / EBP motif at 910 bp of rat iNOS promoter was mutated . rPSMs transfected with the rat iNOS promoter and exposed to 1 % O ( 2 ) for 24 hours had significantly increased wild type iNOS promoter activity . ^^^ Thus , C / EBP beta mediates , at least in part , hypoxia induced iNOS expression in rPSMs . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
IL 1beta activates C / EBP beta and delta in human enterocytes through a mitogen activated protein kinase signaling pathway . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Hypoxia inhibits PPAR gamma 2 nuclear hormone receptor transcription , and overexpression of PPAR gamma 2 or C / EBP beta stimulates adipogenesis under hypoxia . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
C / EBP beta gene inactivation causes both impaired and enhanced gene expression and inverse regulation of IL 12 p 40 and p 35 mRNAs in macrophages . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Promoters utilized in the transcription of previously characterized PRLR exons 1 species hE 1 ( 3 ) ( hPII ) and hE 1 ( N 1 ) ( hP ( N 1 ) ) were found to employ distinct mechanisms for controlling hPRLR transcription . hPIII requires C / EBP beta and Sp1 / Sp3 for basal transcriptional activity , while hP ( N 1 ) activity is conferred by domains containing an Ets element and an NR half site . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
We also show that accessory factors , such as CCAAT / enhancer binding protein beta ( C / EBP beta ) , can recruit CBP to drive transcription . ^^^ Insulin increases protein levels of liver enriched transcriptional inhibitory protein ( LIP ) , an inhibitory form of C / EBP beta , in a phosphatidylinositol 3 kinase dependent manner . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
As this element contains a CAAT / enhancer binding protein ( C / EBP ) motif , the function of C / EBP beta and C / EBP delta was examined . ^^^ IL 1 beta stimulated the expression of C / EBP beta and delta , and the direct binding of C / EBP beta to the C / EBP motif was confirmed . ^^^ Taken together , these results suggest that C / EBP beta and delta may play an important role in the IL 1 beta induced repression of cartilage specific proteins and that expression of matrix proteins will be influenced by the availability of positive and negative trans acting factors . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
We have now unexpectedly found that C / EBP gamma dramatically augments the activity of C / EBP beta in lipopolysaccharide induction of the interleukin 6 and interleukin 8 promoters in a B lymphoblast cell line . ^^^ Studies with chimeric C / EBP proteins implicate the formation of a heterodimeric leucine zipper between C / EBP beta and C / EBP gamma as the critical structural feature required for C / EBP gamma stimulatory activity . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Supershift analysis revealed upregulated DNA binding activity of C / EBP beta but not C / EBP delta . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Both c Jun and CRE binding protein ( CREB ) bound to the CRE , while C / EBP beta bound to NF IL 6 . ^^^ These data indicate that NF kappaB , C / EBP beta , c Jun , and CREB are important in HSP 60 induced expression of COX 2 . ^^^ Mobility shift assays revealed that HSP 60 induced NF kappaB and CRE binding activity , while CCAAT / enhancer binding protein ( C / EBP ) , which binds to NF IL 6 , was constitutively active in the cells . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Reduction of expression of an inhibitory C / EBP beta transcription factor and activation of NF kappa B are observed at the site of inflammation . ^^^ In vitro experiments showed that contact between lymphocytes and macrophages reduced inhibitory C / EBP beta , activated NF kappa B and enhanced HIV 1 replication . ^^^ If contact between lymphocytes and macrophages was prevented , inhibitory C / EBP beta expression was maintained and the HIV 1 long terminal repeat ( LTR ) was not maximally stimulated although NF kappa B was activated . ^^^ Cross linking antibodies abolished inhibitory C / EBP beta expression ; however , the HIV 1 LTR was not maximally stimulated and NF kappa B was not activated . ^^^ Contact between activated lymphocytes and macrophages is necessary to down regulate inhibitory C / EBP beta , thereby derepressing the HIV 1 LTR . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
In brief , Northern blot , gel shift analyses , and transient transfection assays demonstrated that the C / EBP beta ( CCAAT / enhancer binding protein ) transcription factor is the predominant C / EBP family member expressed in the epididymis and gonadotroph cells and is necessary for high levels of Cres expression in these two tissues . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Transcription factors C / EBP beta and delta regulate IL 6 production in IL 1beta stimulated human enterocytes . ^^^ The role of C / EBP beta and delta in enterocyte IL 6 production is not known . ^^^ Finally , overexpression of C / EBP beta and delta in IL 1beta treated Caco 2 cells resulted in a 10 12 fold increase in IL 6 production . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The relative low or high binding affinity of C / EBP beta to sites 1 and 2 in electrophoretic mobility shift ( EMS ) analyses correlated with low or high LTR activity , respectively , in transient expression analyses during both early and late disease stages . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Interferon gamma induces C / EBP beta expression and activity through MEK / ERK and p 38 in T 84 colon epithelial cells . ^^^ We investigated the role of IFN gamma and MAPKs on the expression and activity of the transcription factor CCAAT / enhancer binding protein beta ( C / EBP beta ) in the T 84 colon epithelial cell line . ^^^ IFN gamma induced the expression and activity of C / EBP beta and subsequently increased the secretion of IL 6 from these cells . ^^^ Treatment with the p 38 inhibitor SB 203580 , the MEK 1 and MEK 2 inhibitor U 0126 , or the translational inhibitor cycloheximide inhibited the induction of C / EBP beta and IL 6 by IFN gamma , whereas the MEK 1 inhibitor PD 98059 or the tyrosine kinase inhibitor genistein had no effect . ^^^ These results suggest a role for MEK 2 and p 38 in IFN gamma mediated signal transduction and induction of C / EBP beta expression and activity associated with interleukin 6 ( IL 6 ) secretion in colon epithelial cells . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Impaired hepatocyte DNA synthetic response posthepatectomy in insulin like growth factor binding protein 1 deficient mice with defects in C / EBP beta and mitogen activated protein kinase / extracellular signal regulated kinase regulation . ^^^ The abnormal regenerative response was associated with blunted activation of mitogen activated protein kinase / extracellular signal regulated kinase ( MAPK / ERK ) and a reduced induction of C / EBP beta protein expression posthepatectomy . ^^^ Like cell cycle abnormalities observed in hepatectomized C / EBP beta ( / ) mice , cyclin A and cyclin B 1 expression was delayed and reduced in IGFBP 1 ( / ) livers , whereas cyclin D 1 expression was normal . ^^^ Treatment of IGFBP 1 ( / ) mice with a preoperative dose of IGFBP 1 induced MAPK / ERK activation and C / EBP beta expression , suggesting that IGFBP 1 may support liver regeneration at least in part via its effect on MAPK / ERK and C / EBP beta activities . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The 5 ' flanking region of the IL 8 gene contains potential binding sites for several nuclear factors including activated protein 1 ( AP 1 ) , activated protein 2 ( AP 2 ) , nuclear factor gene binding ( NF kappa B ) , nuclear factor interleukin 6 ( NF IL 6 , also calls CAAT / enhancer binding proteins , C / EBP ) , IFN regulatory factor 1 ( IRF 1 ) , hepatocyte nuclear factor 1 ( HNF 1 ) , and so on . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Immunodepletion , subcellular fractionation , and confocal microscopic analyses showed that the HGF induced C / EBP DNA binding activity depended on nuclear translocation of C / EBP beta . ^^^ Whereas stable transfection of the p 110 catalytic subunit of PI 3 kinase enhanced HGF mediated nuclear translocation of C / EBP beta and DNA binding , stable transfection of p 85 subunit or chemical inhibition of PI 3 kinase completely blocked C / EBP activation . ^^^ In conclusion , HGF induces nuclear translocation of C / EBP beta via the PI 3 kinase pathway and stimulates C / EBP DNA binding and gene transcription and that the PI 3 kinase mediated C / EBP activation by HGF may contribute to cell replication . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Here we show that inhibitory / regulatory C / EBP family proteins , Ig / EBP ( C / EBPgamma ) and CHOP ( C / EBPzeta ) , but not positively functioning NF IL 6 ( C / EBPbeta ) , are constitutively multiubiquitinated and subsequently degraded by the proteasome . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Among these , C / EBP beta induces a variety of cytokines and thus may play a role in the pathogenesis of RCC . ^^^ We studied the activation of C / EBP beta determined by electrophoretic mobility shift assay in nine RCC cell lines and 44 tissue samples . ^^^ Six cell lines showed an activation of C / EBP beta , whereas three cell lines did not , and two of these three had no expression at all of C / EBP beta protein . ^^^ Locally advanced cases had a significantly higher rate of increased C / EBP beta activity ( 5 of 8 = 62 . 5 % in advanced cases versus 7 of 36 = 19 . 4 % in localized cases ) . ^^^ Especially , all four cases with renal vein invasion had an increased C / EBP beta activity . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Gel shift assay and immunoblot analysis of subcellular fractions revealed that PD 98059 caused nuclear translocation of C / EBP beta and increased C / EBP DNA binding , which was super shifted with anti C / EBP beta antibody . ^^^ Flavone , a backbone structure of PD 98059 , also induced nuclear translocation of C / EBP beta and C / EBP mediated rGSTA 2 gene induction . ^^^ Inhibition of phosphatidylinositol 3 kinase abolished C / EBP beta mediated rGSTA 2 induction by PD 98059 . ^^^ These results provide evidence that PD 98059 and flavone induce nuclear translocation of C / EBP beta and activate the C / EBP binding site in the rGSTA 2 gene , which constitutes the distinct pathway for the enzyme induction irrespective of the inhibition of MKK1 / ERK activity . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
C / EBP homologous protein ( CHOP ) up regulates IL 6 transcription by trapping negative regulating NF IL 6 isoform . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Transcriptional inhibition of interleukin 8 expression in tumor necrosis factor tolerant cells : evidence for involvement of C / EBP beta . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Expression of CAAT enhancer binding protein beta ( C / EBP beta ) in cervix and endometrium . ^^^ C / EBP beta has been shown to play an important role in mediating the effects of the LH / hCG in ovarian development . ^^^ C / EBP beta was also found increased in ovarian cancer and correlated to ovarian cancer progression . ^^^ DESIGN : We assessed the C / EBP beta expression pattern in the normal and neoplastic conditions of the female genital tract by immunostaining the normal cervix 10 cases , squamous intraepithelial lesion ( SIL ) 10 , invasive squamous carcinomas of cervix 10 cases , normal endometrial tissue 10 cases , and invasive endometrial adenocarcinomas carcinomas 10 cases . ^^^ CONCLUSION : C / EBP beta is more likely to be preferentially expressed in endometrial adenocarcinoma as compared to benign endometrial tissue . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
A nucleoprotein complex containing Sp 1 , C / EBP beta , and HMGI Y controls human insulin receptor gene transcription . ^^^ Here we provide evidence that transcriptional activation of the human IR promoter requires the assembly of a transcriptionally active multiprotein DNA complex which includes , in addition to HMGI Y , the ubiquitously expressed transcription factor Sp 1 and the CCAAT enhancer binding protein beta ( C / EBP beta ) . ^^^ Functional integrity of this nucleoprotein complex is required for full transactivation of the IR gene by Sp 1 and C / EBP beta in cells readily expressing IRs . ^^^ We show that HMGI Y physically interacts with Sp 1 and C / EBP beta and facilitates the binding of both factors to the IR promoter in vitro . ^^^ Repression of HMGI Y function adversely affects both Sp 1 and C / EBP beta induced transactivation of the IR promoter . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Binding of NF kappa B subunit c Rel to DNA nucleated the concerted binding of transcription factors AP 1 and C / EBP beta to the 5 ' regulatory region of bfl 1 . ^^^ Chromatin immunoprecipitation analyses demonstrated that T cell activation triggers in vivo binding of endogenous c Rel , c Jun , C / EBP beta , and HMG IC to the bfl 1 regulatory region , coincident with selective recruitment of coactivators TAFII 250 and p 300 , SWI / SNF chromatin remodeling factor component BRG 1 , and basal transcription factors TATA binding protein ( TBP ) and TFIIB , as well as hyperacetylation of histones H 3 and H 4 . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Gel shift analysis revealed that four LIP and LAP containing complexes ( a d ) bind to the C / EBP site . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Our results demonstrate that C . pneumoniae activates C / EBP beta , NF kappaB , and the GR in infected cells . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The tumor necrosis factor alpha dependent activation of the human mediterranean fever ( MEFV ) promoter is mediated by a synergistic interaction between C / EBP beta and NF kappaB p 65 . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
CCAAT / enhancer binding protein beta ( C / EBP beta ) activator isoforms , C / EBP beta 1 and C / EBP beta 2 , differ by only 23 amino acids in the human ; however , evidence is accumulating that these transcription factors are functionally distinct . ^^^ Here we demonstrate that C / EBP beta 1 , but not C / EBP beta 2 , is conjugated to the small ubiquitin like modifier ( SUMO ) family members , SUMO 2 and SUMO 3 despite the fact that the SUMO target consensus is present in both isoforms of this transcription factor . ^^^ This conjugation is dependent on the integrity of the extreme N terminus of C / EBP beta 1 and requires lysine 173 in the human protein . ^^^ Furthermore , mutation of this lysine relieves the repression of the cyclin D 1 promoter by C / EBP beta 1 without altering the subnuclear localization of C / EBP beta 1 . ^^^ The sumoylation of C / EBP beta 1 is likely to be important in the functional differences observed between C / EBP beta 1 and C / EBP beta 2 . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Regulation of elastin gene transcription by interleukin 1 beta induced C / EBP beta isoforms . ^^^ IL 1beta treatment increased CCAAT / enhancer binding protein ( C / EBP ) beta mRNA and protein levels including liver enriched activating protein ( LAP ) and liver enriched inhibitory protein ( LIP ) , which was cycloheximide sensitive . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Employing electrophoretic mobility shift assays , we demonstrated that increased functional binding of CCAAT / enhancer binding protein ( C / EBP ) alpha , C / EBPbeta and upstream stimulatory factor 2 to the CRE and C / EBPalpha and C / EBPbeta to the NF IL 6 site were , at least in part , responsible for MECM induced COX 2 expression in ESC . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
PPARs also control the expression of genes implicated in the inflammatory response via negative interference with different inflammatory pathways , such as NFkappaB , AP 1 , C / EBP beta , STAT 1 and NFAT . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The upstream sequence between 314 bp and +54 bp had the inducible activity by BCG , which contained CCAAT / enhancer binding protein beta ( C / EBP beta ) , activator protein 1 ( AP 1 ) , and CP 2 cis element . ^^^ The sequence ( 314 / +54 ) containing C / EBP beta , AP 1 , and CP 2 binding sites in the upstream of hBD 1 is involved in this induction . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Transient transfection studies and in vitro analyses of IL 1 induced cox 2 transcription in a number of cell types have indicated regulation by either nuclear factor kappa B ( NF kappa B ) or CCAAT / enhancer binding protein ( C / EBP beta ) , or both acting cooperatively . ^^^ To determine the mechanisms of COX 2 ( cyclooxygenase or prostaglandin endoperoxide synthase ) induction in cultured human myometrial cells in situ , we examined the cross linking of the RelA subunit of NF kappa B and C / EBP beta to the cox 2 promoter and flanking sequences . ^^^ As a control , we inspected the interaction of these transcription factors with the IL 8 gene , which has been shown in other cell types to be activated by the cooperative interaction of NF kappa B and C / EBP beta . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
This region contains putative consensus sequences for the Stat and NF IL 6 ( C / EBP beta ) transcription factors . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Cell transformation by the 5 myc oncogene abrogates c Myc / Max mediated suppression of a C / EBP beta dependent lipocalin gene . ^^^ The promoter region contains consensus binding sites for the transcriptional regulators Myc and C / EBP beta . ^^^ Transcriptional activation of Q 83 is principally dependent on C / EBP beta , but is blocked in normal cells by the endogenous c Myc / Max / Mad transcription factor network . ^^^ In 5 myc transformed cells , high level expression of the 5 Myc protein and formation of highly stable 5 Myc / Max heterodimers leads to abrogation of Q 83 gene suppression and activation by C / EBP beta . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Differential activation of a C / EBP beta isoform by a novel redox switch may confer the lipopolysaccharide inducible expression of interleukin 6 gene . ^^^ C / EBP beta , a member of the CCAAT / enhancer binding protein ( C / EBP ) family , is one of the key transcription factors responsible for the induction of a wide array of genes , some of which play important roles in innate immunity , inflammatory response , adipocyte and myeloid cell differentiation , and the acute phase response . ^^^ Three C / EBP beta isoforms ( i . e . ^^^ C / EBP beta is known to induce interleukin ( IL ) 6 gene when P388D1 cells are treated with lipopolysaccharide ( LPS ) . ^^^ Exactly how the transcriptional activities of C / EBP beta isoforms are involved in the regulation of the IL 6 gene remains unclear . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Both the NF kappaB and CCAAT / enhancer binding protein ( C / EBP ; NF IL 6 ) sites in the IL 6 promoter are important for cooperative gene expression , but NF kappaB does not appear to be the direct target of the combined signal . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Computer consensus match analysis indicated that the nucleotides between 63 bp to 50 bp was the potential binding site for C / EBP beta . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The effect of sodium butyrate on aromatase expression in breast tumor fibroblasts was mediated by inhibition of phosphorylation of ATF 2 , and hence , the inhibition of binding of a transcriptional complex containing phosphorylated ATF 2 , C / EBP beta and CREB binding protein ( CBP ) to promoter II / I . 3 regulatory region . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Ras induces mediator complex exchange on C / EBP beta . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Expression pattern of the CCAAT / enhancer binding protein C / EBP beta in gestational trophoblastic disease . ^^^ C / EBP beta , the most important member of the C / EBP family , was shown recently to be expressed in the normal human placenta where it is localized in villous syncytiotrophoblast and in the extravillous ( intermediate ) trophoblast but not the villous cytotrophoblast . ^^^ The purpose of this study was to investigate the expression pattern of C / EBP beta in gestational trophoblastic disease ( GTD ) which has not been studied so far . ^^^ All our tested specimens showed positivity for C / EBP beta . ^^^ The strongest C / EBP beta expression could be observed in villous syncytiotrophoblast and in the trophoblast proliferations on the villous surface of hydatidiform moles ; villous cytotrophoblast was negative . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The signals of C / EBP beta in hepatocytes were weak in fetal liver , but became stronger with postnatal development . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
This review highlights the importance of C / EBP beta and NF kappa B in COX 2 transcriptional activation by proinflammatory mediators and as targets of inhibition by endogenous molecules such as melatonin and natural products including salicylate and polyphenols . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Similarly cotransfection of GR and C / EBP beta or C / EBP delta increases the promoter activity of reporter construct containing nucleoside A at 217 . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
These data implicate protein kinase A as the major regulator of CEBPB isoform distribution and CEBP mediated transactivation in granulosa cells . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Myxoid liposarcoma FUS DDIT 3 fusion oncogene induces C / EBP beta mediated interleukin 6 expression . ^^^ We here report that the NFkappaB and C / EBP beta controlled gene IL 6 is upregulated in DDIT 3 and FUS DDIT 3 expressing fibrosarcoma cell lines and in myxoid liposarcoma cell lines . ^^^ Knockdown experiments using siRNA against CEBPB transcripts showed that the effect of FUS DDIT 3 on IL 6 expression is C / EBP beta dependent . ^^^ Chromatin immunoprecipitation revealed direct interaction between the IL 6 promoter and the C / EBP beta protein . ^^^ We demonstrate for the first time that DDIT 3 and FUS DDIT 3 show opposite transcriptional regulation of IL 8 and suggest that FUS DDIT 3 may affect the synergistic activation of promoters regulated by C / EBP beta and NFkappaB . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Cooperative interaction of C / EBP beta and Tat modulates MCP 1 gene transcription in astrocytes . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Point mutations in C / EBP beta binding domains of the HIV 1 LTR negative regulatory element ( NRE ) abolished promoter repression . ^^^ Contact between lymphocytes and AM reduced inhibitory C / EBP beta , activated NF kappaB and enhanced HIV 1 replication . ^^^ If contact between lymphocytes and macrophages was prevented , inhibitory C / EBP beta expression was maintained and the HIV 1 long terminal repeat ( LTR ) was not maximally stimulated although NF kappaB was activated . ^^^ Cross linking antibodies abolished inhibitory C / EBP beta expression ; however , the HIV 1 LTR was not maximally stimulated and NF kappaB was not activated . ^^^ Contact between activated lymphocytes and macrophages is necessary to downregulate inhibitory C / EBP beta , thereby derepressing the HIV 1 LTR . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Induction of C / EBP beta and GADD 153 expression by dopamine in human neuroblastoma cells . ^^^ Expression of CCAAT / enhancer binding protein beta ( C / EBP beta ) and growth arrest DNA damage inducible 153 / C / EBP beta homology protein ( GADD153 / CHOP ) increased after incubation of human neuroblastoma SH SY5Y cells with a range of dopamine concentrations . ^^^ Dopamine ( 100 microM ) caused an increase in C / EBP beta expression between 2 and 12 h of treatment , with no evident intracellular morphological changes . ^^^ The expression of alpha synuclein , the main protein of Lewy bodies in Parkinson ' s disease and other neurological disorders , increased with a profile similar to C / EBP beta . ^^^ In addition , overexpression of C / EBP beta caused a concomitant increase in the expression of alpha synuclein but not of GADD 153 . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
In turn , TNF alpha stimulates oxidative stress , NO synthesis , and phosphorylation of C / EBP beta within its nuclear localization signal ( NLS ) . ^^^ Consequently , C / EBP beta is exported from the nucleus , preventing it to act as a transcriptional factor on the albumin gene . ^^^ Antioxidants , NOS inhibitors . and dominant negative , nonphosphorylatable C / EBP beta peptides block phosphorylation of C / EBP beta within the NLS and its nuclear export as well as rescue the abnormal albumin gene expression , suggesting potential therapeutic interventions . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
For instance , during inflammation , released cytokines induce repressive C / EBPbeta liver inhibitory protein ( LIP ) , which antagonizes constitutive C / EBP transactivators [ C / EBPalpha and C / EBPbeta liver activating protein ( LAP ) ] , down regulating genes such as CYP3A4 . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
C / EBP beta was the dominant C / EBP in the airway epithelium in all groups . ^^^ CONCLUSIONS : We hypothesize that this lack of increase in C / EBP beta activity renders the epithelium incompetent of efficient regeneration and more sensitive to infection , suggesting a previously unknown role for C / EBPs in the pathogenesis of CB and COPD . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
We have previously reported that chronic activation of phosphatidylinositol 3 kinase ( PI 3 kinase ) by the overexpression of membrane targeted p110CAAX induced proinflammatory gene expression in rat vascular smooth muscle cells ( VSMCs ) through the induction of CCAAT / enhancer binding protein beta ( C / EBP beta ) and C / EBP delta . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Adiponectin transcription was increased 3 fold in cells that over expressed constitutively active C / EBP beta . ^^^ The present data indicate that the putative response elements for SREBP and C / EBP are required for human adiponectin promoter activity , and that suppression by TNF alpha may , at least in part , be associated with inactivation of C / EBP beta . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Notably , stress induced transcription factors such as ATF 3 , ATF 4 , CHOP , and C / EBP beta were robustly up regulated , yet exhibited unique kinetic patterns . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
We assumed a possible contribution of CCAAT / enhancer binding protein ( C / EBP ) beta , a transcription factor implicated in the regulation of plasma proteins in the liver , to the regulation of AFP production and to the expression of other plasma proteins in yolk sac tumor cells because our immunohistochemical analysis revealed nuclear expression of C / EBP beta in human yolk sac tumors . ^^^ Overexpression of C / EBP beta in a rat yolk sac tumor cell line , AT 2 TC , increased production of AFP and other plasma proteins , including albumin , alpha 1 antitrypsin , hepatoglobin , and transferrin . ^^^ The induction of this protein expression was only evident in xenografts , where C / EBP beta was phosphorylated and the activating isoform of C / EBP beta was relatively predominant . ^^^ These results indicate that C / EBP beta plays a role in the production of plasma proteins of yolk sac tumors . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
A transcriptional role for C / EBP beta in the neuronal response to axonal injury . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The CCAAT / enhancer binding protein ( C / EBP ) beta isoforms liver enriched activator protein ( LAP ) and truncated dominant negative liver enriched inhibitory protein ( LIP ) differentially regulate adipogenesis . ^^^ Immunoblot , gel shift , and Southwestern analyses revealed that oltipraz enhanced the levels of nuclear LIP and LAP and their binding to the C / EBP binding site . ^^^ Likewise , LAP mediated luciferase gene transactivation was inhibited by oltipraz , as was observed by cotransfection of a dominant negative mutant form of C / EBP . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
GW 501516 increased not only the binding activity of C / EBP to the NF IL 6 site in the EP 4 promoter , which was prevented by the inhibitor of PI 3 K , but also increased C / EBPbeta protein in a dose and PPARbeta / delta dependent manner . ^^^ The increase in transcription of the EP 4 gene by PPARbeta / delta agonist was associated with increased C / EBP binding activity in the NF IL 6 site of EP 4 promoter region and C / EBPbeta protein expression that were mediated through both PI 3 K / Akt and PPARbeta / delta signaling pathways . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
L13a , L 29 , and L 37 ) , and transcription factors ( e . g . , Jun B , Jun D , and C / EBP beta ) were underexpressed . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
The binding of CCAAT / enhancer binding protein ( C / EBP ) beta and C / EBPdelta to the CRE and NF IL 6 elements , as well as the recruitment of CBP and the enhancement of histone H 3 and H 4 acetylation on the COX 2 promoter was enhanced by MG 132 . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Systemic delivery of full length C / EBP beta / liposome complex suppresses growth of human colon cancer in nude mice . ^^^ C / EBP beta ( CCAAT / enhancer binding protein beta ) is an important transcription factor involved in cellular proliferation and differentiation . ^^^ Overexpression of the full length C / EBP beta protein results in cellular growth arrest and apoptosis . ^^^ Using a nonviral liposome as carrier , we delivered the full length C / EBP beta expression plasmid , pCN , into nude mice bearing CW 2 human colon cancer tumors via tail vein . ^^^ Experimental gene therapy showed that a strong suppression of tumor growth was observed in the pCN treated mice , and such suppression was due to the overexpression of C / EBP beta , leading to the increased apoptosis in tumors of pCN treated mice . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Cytokines modulate transcription mediated by the HIV 1 long terminal repeat ( LTR ) via multiple signal transduction pathways with resulting recruitment of numerous transcription factors , including NFkappaB , C / EBP , AP 1 , TCF 1alpha , NF IL 6 and ISGF 3 . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Because expression of CEBPB ( and CEBPA ) is low in the blast crisis ( BC ) stage of chronic myelogenous leukemia ( CML ) and is inversely correlated with BCR / ABL tyrosine kinase levels , these findings point to the therapeutic potential of restoring C / EBP activity in CML BC and , perhaps , other types of acute leukemia . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
IL 1beta stimulation of chondrocytes increased binding of C / EBP beta to the MMP 1 C / EBP site . ^^^ Extracellular signal regulated kinase ( ERK ) pathway dependent phosphorylation of C / EBP beta on threonine 235 activates this transcription factor . ^^^ Here we show that IL 1beta stimulation of chondrocytes induced phosphorylation of C / EBP beta on threonine 235 , and that the ERK pathway inhibitor PD 98059 reduced this phosphorylation . ^^^ Our findings demonstrate a novel role for C / EBP beta in IL 1beta induced connective tissue disease and define a new nuclear target for the ERK pathway in MMP 1 gene activation . . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
C / EBP beta ( CCAAT / enhancer binding protein beta ) is a transcriptional factor that belongs to the basic region leucine zipper class DNA binding proteins and plays a role in cell differentiation and inflammatory reactions . ^^^ C / EBP beta induces a variety of cytokines and thus may play a role in the pathogenesis of glioma . ^^^ In this study , we investigated the relationship between C / EBP beta expression , tumor histology , and prognosis in glioma . ^^^ The expression of C / EBP beta mRNA was examined with quantitative real time PCR and protein expression was examined with immunohistochemical techniques in 47 glioma tissue samples . ^^^ Expression of C / EBP beta mRNA and protein was markedly increased in high grade glioma compared with low grade glioma . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
C / EBP beta blocks p 65 phosphorylation and thereby NF kappa B mediated transcription in TNF tolerant cells . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
Expression of CCAAT / enhancer binding protein beta ( C / EBP beta ) isoforms was examined by Western blot analysis . ^^^ Innate immune responses involving interferon beta and the dominant negative isoform of C / EBP beta were induced at this time . ^^^
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
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Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
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Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
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Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
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Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
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Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
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Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
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Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
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Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
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Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
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Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
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Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
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Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
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Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
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Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
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Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
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Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
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Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
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Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
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Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
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Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
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Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
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Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
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Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
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Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA
Interacting proteins: P49715 and P17676	Pubmed	SVM Score :0.0
NA