| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.98159881 |
| On the other hand , ROMK 1 has been shown to colocalize with NHERF 2 , a protein mediating targeting and trafficking of transport proteins into the cell membrane . 0.98159881^^^ |
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| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| These results suggest that KIR2 . 3 , but not KIR1 . 1 or KIR2 . 1 , is subject to regulation , both by PKC activation and as a consequence of low [ Na+ ] o . ^^^ |
|
| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| The epithelial sodium channel ( ENaC ) and the secretory potassium channel ( Kir1 . 1 / ROMK ) are expressed in the apical membrane of renal collecting duct principal cells where they provide the rate limiting steps for Na ( + ) absorption and K ( + ) secretion . ^^^ |
|
| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| Loss of function mutations in the K channel , ROMK ( Kir1 . 1 ; KCNJ 1 ) , cause Bartter syndrome , a genetically heterogeneous disorder characterized by severe reduction in salt absorption by the TAL , Na wasting , polyuria , and hypokalemic alkalosis . ^^^ |
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| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| The current data on the developmental expression and functions of ENaC Na ( + ) channels , the CFTR , ClC 2 Cl ( ndash ; ) channels , L type Ca ( 2+ ) channels , P 2 purinoceptors , and the Kir6 . 1 / SUR2 , ROMK ( Kir1 . 1 ) , and Kv K ( + ) channels are presented . . ^^^ |
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| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| In whole cell patch clamp recordings wildtype rat ROMK 1 exhibited K+ currents of > 1 nA at a membrane potential of 100 mV when transfected into COS 7 kidney cells . ^^^ |
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| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| On the other hand , Bartter ' s syndrome , characterized by defective salt reabsorption by the ascending limb of Henle ' s loop , is associated with mutations in either the NKCC 2 gene encoding the loop ' s 1Na+ 1K+ 2Cl cotransporter , or in the ROMK gene , which allows K+ recycling in the loop to occur from cell to lumen , making Na+ reabsorption via the cotransporter possible . ^^^ |
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| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| These results suggest that the postnatal increases in a principal cell apical SK and Na+ channel activity are mediated , at least in part , by increases in abundance of ROMK message and protein and ENaC subunit proteins . . ^^^ |
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| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| Reverse transcription polymerase chain reaction experiments showed that they expressed the mNKCC 2 electroneutral Na+ K+ Cl cotransporter and ROM K 1 and ROMK 2 potassium channel mRNA . ^^^ |
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| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| In addition , NHERF 2 was strongly expressed in collecting duct principal cells , where it colocalized with ROMK . ^^^ These results indicate a striking difference in the nephron distribution of NHERF and NHERF 2 and suggests NHERF is most likely to be the relevant biological regulator of NHE 3 in the proximal tubule , while NHERF 2 may interact with ROMK or other targets in the collecting duct . ^^^ |
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| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| In addition , dietary Na ( + ) levels were varied to determine whether ROMK abundance was also affected under other conditions known to alter TAL transport . ^^^ |
|
| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NHERF 2 was seen in glomeruli , the renal vasculature , and collecting duct cells , where it colocalized with ROMK . ^^^ |
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| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| For this purpose we analysed messenger ribonucleic acid ( mRNA ) levels for the bumetanide sensitive Na+K+2Cl cotransporter ( BSC 1 ) , the thiazide sensitive Na+Cl cotransporter ( TSC ) , the kidney specific inwards rectifier K+ channel ( ROMK ) , the amiloride sensitive epithelial Na+ channel ( ENaC ) and the kidney specific Cl channel ClC K 2 , in the cortex and inner and outer medulla of kidneys from male Sprague Dawley rats fed a high ( 8 % w / w ) , normal ( 0 . 6 % ) or low ( 0 . 02 % ) salt diet or treated chronically with subcutaneous infusions of furosemide ( 12 mg / kg per day ) . ^^^ |
|
| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| There were no differences in band densities for the renal outer medullary potassium channel ( ROMK ) , the type 3 Na H exchanger ( NHE 3 ) , the alpha subunit of Na , K ATPase in the OM ; or for UT A 1 , UT A 2 or UT A 4 in outer or inner medulla . ^^^ |
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| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| There was no difference in the mRNA expressions of thiazide sensitive NaCl cotransporter , epithelial Na channel ( ENaC ) , aquaporin 2 , ROMK , and NaKATPase . ^^^ |
|
| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| Mutations have been identified in the NKCC 2 ( Na ( + ) K ( + ) 2Cl ( ) ) cotransporter and ROMK potassium channel , which cooperate in the process of apical sodium chloride uptake , and ClC Kb chloride channels , which mediate basolateral chloride release . ^^^ |
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| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| ROMK deficient mice that survived beyond weaning grew to adulthood ; however , they had metabolic acidosis , elevated blood concentrations of Na ( + ) and Cl ( ) , reduced blood pressure , polydipsia , polyuria , and poor urinary concentrating ability . ^^^ |
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| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| Loss of function mutations in the human ROMK gene cause Bartter ' s syndrome with renal Na ( + ) wasting , consistent with the role of this channel in apical K ( + ) recycling in the TAL that is crucial for NaCl reabsorption . ^^^ |
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| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND : Coordinated expression of ROMK ( luminal K+ channel in the thick ascending limb and the collecting duct ) and Na+ , K+ ATPase has been demonstrated to be involved in the postnatal development of renal K+ excretion ; however , the developmental expression of the basolateral K+ channel Kir7 . 1 is unknown . ^^^ RNase protection analysis was used to elucidate the expression of Kir7 . 1 and Na+ , K+ ATPase mRNA in pre and postnatal rats , and the expression of Kir7 . 1 and ROMK mRNA at 7 , 14 , and 21 days . ^^^ |
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| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| Here we identify the Na / H exchange regulatory factors , NHERF 1 and NHERF 2 , as PDZ domain interaction partners of the ROMK channel . ^^^ As measured by antibody binding of external epitope tagged forms of Kir 1 . 1 in intact cells , NHERF 1 or NHERF 2 coexpression increased cell surface expression of ROMK . ^^^ Both NHERF proteins contain two PDZ domains ; recombinant protein protein binding assays and yeast two hybrid studies revealed that ROMK preferentially associates with the second PDZ domain of NHERF 1 and with the first PDZ domain of NHERF 2 , precisely opposite of what has been reported for CFTR . ^^^ Consistent with the scaffolding capacity of the NHERF proteins , coexpression of NHERF 2 with ROMK and CFTR dramatically increases the amount of ROMK protein that coimmunopurifies and functionally interacts with CFTR . ^^^ |
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| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| In oocytes , SGK 1 activates various renal transport proteins including Na+ / glucose cotransporter SGLT 1 , Na+ coupled dicarboxylate transporter NaDC 1 , epithelial Ca+ channel TRPV 5 , renal outer medullary K+ channel ROMK and voltage gated K+ channels KCNE1 / KCNQ1 and Kv1 . 3 . ^^^ |
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| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| Conversely , impaired function of the Na+ , K+ , 2Cl cotransporter ( NKCC 2 ) , the renal outer medullary K+ channel ( ROMK 1 ) , and the renal epithelial Cl channel ClCKb / Barttin causes Bartter syndrome and defective Na+ , Cl+ cotransporter ( NCCT ) Gitelman syndrome , salt wasting disorders with hypotension . ^^^ The second common gene variant ( prevalence , 3 % 5 % in unselected Caucasians ) , to be discussed in more detail , affects the serum and glucocorticoid inducible kinase SGK 1 , a kinase upregulated by mineralocorticoids and enhancing the activity of ENaC , ROMK , and Na+ / K+ATPase . ^^^ |
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| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| Western blot analysis showed up regulation of expression of the alpha subunit of the epithelial sodium channel ( alpha ENaC ) , the beta subunit of the epithelial sodium channel ( beta ENaC ) , sodium / hydrogen exchanger ( NHE ) 3 , and the renal outer medulla K ( + ) channel ( ROMK ) in HF rats , whereas expression of the gamma subunit of the epithelial sodium channel and the alpha 1 subunit of Na ( + ) K ( + ) ATPase remained unchanged . ^^^ |
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| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| The expression of NHE 3 , Na K ATPase , NKCC 2 , ROMK , NCC , alpha , beta and gamma ENaC , and CaSR was examined in the kidney by immunoblotting and immunohistochemistry . ^^^ In the inner stripe of outer medulla ( ISOM ) in GM 80 rats , NKCC 2 and Na K ATPase expression was decreased , whereas CaSR was upregulated , and NHE 3 and ROMK expression remained unchanged . ^^^ In GM 40 rats , NKCC 2 expression was decreased in the cortex and ISOM , whereas NHE 3 , Na K ATPase , CaSR , ROMK , and NCC abundance was unchanged in both cortex and ISOM . ^^^ |
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| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| SGKs activate ion channels ( e . g . , ENaC , TRPV 5 , ROMK , Kv1 . 3 , KCNE1 / KCNQ1 , GluR 1 , GluR 6 ) , carriers ( e . g . , NHE 3 , GLUT 1 , SGLT 1 , EAAT 1 5 ) , and the Na+ K+ ATPase . ^^^ |
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| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| A quantitative analysis shows that an external ion site in the ROMK 1 pore binds various alkali metal ions ( Na+ , K+ , Rb+ , and Cs+ ) with different affinities , which can in turn be altered by the binding of different permeating ions at an internal site through a nonelectrostatic mechanism . ^^^ Furthermore , the external and internal ion binding sites in the ROMK 1 pore appear to have different ion selectivity : the external site selects strongly against the smaller Na+ , but only modestly among the three larger ions , whereas the internal site interacts quite differently with the larger K+ and Rb+ ions . . ^^^ |
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| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| Na ( + ) sensitivity of ROMK 1 K ( + ) channel : role of the Na ( + ) / H ( + ) antiporter . ^^^ To examine the extracellular Na ( + ) sensitivity of a renal inwardly rectifying K ( + ) channel , we performed electrophysiological experiments on Xenopus oocytes or a human kidney cell line , HEK 293 , in which we had expressed the cloned renal K ( + ) channel , ROMK 1 ( Kir 1 . 1 ) . ^^^ When extracellular Na ( + ) was removed , the whole cell ROMK 1 currents were markedly suppressed in both the oocytes and HEK 293 cells . ^^^ Single channel ROMK 1 activities recorded in the cell attached patch on the oocyte were not affected by removal of Na ( + ) from the pipette solution . ^^^ However , macro patch ROMK 1 currents recorded on the oocyte were significantly suppressed by Na ( + ) removal from the bath solution . ^^^ |
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| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| In addition , SGK 1 together with NHERF 2 stimulates the K+ channel ROMK 1 , suggesting a broader role of SGK 1 in regulation of ion transport . . ^^^ |
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| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| The serum and glucocorticoid inducible kinase SGK 1 stimulates the Na+ channels ENaC and SCN5A , the K+ channels ROMK 1 , Kv1 . 3 , and KCNE1 / KCNQ1 , the cation conductance induced by 4F2 / LAT1 and the chloride conductance induced by CFTR . ^^^ |
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| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| Abbreviations : K : potassium , Na : sodium ; Cl : chloride ; BS : Bartter syndrome ; PHA : pseudohypoaldosteronism ; NKCC 2 : bumetanide sensitive Na K 2Cl co transporter ; ROMK : inwardly rectifying K channel ; ClC Kb : basolateral Cl channel ; TAL : thick ascending limb of Henle ' s loop ; TSC : thiazide sensitive NaCl co transporter ; CCD : cortical collecting duct ; ENaC : epithelial Na channel . . ^^^ |
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| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| The renal outer medullary K ( + ) channel ROMK 1 is upregulated by the serum and glucocorticoid inducible kinase SGK 1 , an effect potentiated by Na ( + ) / H ( + ) exchanger regulating factor NHERF 2 . ^^^ Wild type ROMK 1 , [ S44A ] ROMK 1 , and [ S44D ] ROMK 1 were expressed in Xenopus oocytes with or without constitutively active [ S422D ] SGK 1 and NHERF 2 , and K ( + ) current ( 1 ( KR ) ) determined . ^^^ Coexpression of [ S422D ] SGK 1 and NHERF 2 enhanced the activity of ROMK 1 , [ S44A ] ROMK 1 and [ S44D ] ROMK 1 , but led to a significant shift of pK ( a ) only in wild type ROMK 1 ( 6 . 95+ / 0 . 03 ) . ^^^ |
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| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| The serum and glucocorticoid inducible kinase SGK 1 stimulates the renal outer medullary K ( + ) channel ROMK 1 in the presence of the Na ( + ) / H ( + ) exchanger regulating factor NHERF 2 . ^^^ According to chemiluminescence and electrophysiology , deletion of the second PDZ domain of NHERF 2 or the putative PDZ binding motif on ROMK 1 abrogates the stimulating effect of SGK 1 on ROMK 1 protein abundance in the plasma membrane and K ( + ) current . . ^^^ |
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| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P48048 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
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