| Interacting proteins: P48048 and O14745 |
Pubmed |
SVM Score :0.0 |
| Differential renal distribution of NHERF isoforms and their colocalization with NHE 3 , ezrin , and ROMK . ^^^ In addition , NHERF 2 was strongly expressed in collecting duct principal cells , where it colocalized with ROMK . ^^^ These results indicate a striking difference in the nephron distribution of NHERF and NHERF 2 and suggests NHERF is most likely to be the relevant biological regulator of NHE 3 in the proximal tubule , while NHERF 2 may interact with ROMK or other targets in the collecting duct . ^^^ |
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| Interacting proteins: P48048 and O14745 |
Pubmed |
SVM Score :0.0 |
| NHERF 2 was seen in glomeruli , the renal vasculature , and collecting duct cells , where it colocalized with ROMK . ^^^ |
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| Interacting proteins: P48048 and O14745 |
Pubmed |
SVM Score :0.0 |
| Here we identify the Na / H exchange regulatory factors , NHERF 1 and NHERF 2 , as PDZ domain interaction partners of the ROMK channel . ^^^ Characterization of the basis and consequences of NHERF association with ROMK reveals a PDZ interaction dependent trafficking process and a coupling mechanism for linking ROMK to a channel modifier protein , the cystic fibrosis transmembrane regulator ( CFTR ) . ^^^ As measured by antibody binding of external epitope tagged forms of Kir 1 . 1 in intact cells , NHERF 1 or NHERF 2 coexpression increased cell surface expression of ROMK . ^^^ Channel interaction with NHERF proteins and effects of NHERF on ROMK localization were dependent on the presence of the PDZ domain binding motif in ROMK . ^^^ Both NHERF proteins contain two PDZ domains ; recombinant protein protein binding assays and yeast two hybrid studies revealed that ROMK preferentially associates with the second PDZ domain of NHERF 1 and with the first PDZ domain of NHERF 2 , precisely opposite of what has been reported for CFTR . ^^^ |
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| Interacting proteins: P48048 and O14745 |
Pubmed |
SVM Score :0.0 |
| NA |
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