| Interacting proteins: P09237 and P48023 |
Pubmed |
SVM Score :0.0 |
| RESULTS : Here we report that the matrix metalloproteinase matrilysin can process recombinant and cell associated FasL to sFasL , and that matrilysin generated sFasL was effective at inducing apoptosis in a target epithelial cell population . ^^^ In the involuting mouse prostate , FasL and matrilysin colocalized to the cell surface in a restricted population of epithelial cells . ^^^ Mice deficient in matrilysin demonstrated a 67 % reduction in the apoptotic index in the involuting prostate compared with wild type animals , implicating matrilysin in this FasL mediated process . ^^^ CONCLUSIONS : The results show that a functional form of sFasL was generated by the action of the metalloproteinase matrilysin , and suggest that matrilysin cleavage of FasL is an important mediator of epithelial cell apoptosis . . ^^^ The metalloproteinase matrilysin proteolytically generates active soluble Fas ligand and potentiates epithelial cell apoptosis . ^^^ |
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| Interacting proteins: P09237 and P48023 |
Pubmed |
SVM Score :0.0 |
| In the human HBL 100 and murine NMuMG cell lines that represent early stages of tumor progression and that express both Fas ligand and its receptor , exposure to matrilysin results in cell death that can be blocked by FasL neutralizing antibodies . ^^^ |
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| Interacting proteins: P09237 and P48023 |
Pubmed |
SVM Score :0.0 |
| Cleavage of FasL by MMP 7 ( matrilysin ) protected the parental SK N MC cells from Dox , whereas inhibition of MMP 7 activity increased their sensitivity . ^^^ |
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| Interacting proteins: P09237 and P48023 |
Pubmed |
SVM Score :0.0 |
| In cell culture models , acute exposure of cells coexpressing Fas and Fas ligand ( FasL ) to matrilysin induces apoptosis , whereas chronic exposure to matrilysin selects for apoptosis resistant cells . ^^^ Mammary tissue from multiparous matrilysin expressing mice showed decreased FasL expression , suggesting that loss of FasL is at least one mechanism of matrilysin induced resistance to apoptosis . ^^^ |
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| Interacting proteins: P09237 and P48023 |
Pubmed |
SVM Score :0.0 |
| Identification of novel matrix metalloproteinase 7 ( matrilysin ) cleavage sites in murine and human Fas ligand . ^^^ |
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| Interacting proteins: P09237 and P48023 |
Pubmed |
SVM Score :0.0 |
| METHODS : We investigated , by flow cytometric analysis , the presence of FasL on spermatozoa from normozoospermic and teratozoospermic subjects and , by western blot , the presence of sFasL and matrilysin in the seminal plasma of the same samples as well as on samples from azoospermic subjects . ^^^ Presence of membrane and soluble forms of Fas ligand and of matrilysin ( MMP 7 ) activity in normal and abnormal human semen . ^^^ BACKGROUND : The aim of this study is to shed some light on the role of the Fas system in human semen , by investigating whether there is an association between the expression of the molecules regulating the Fas system [ membrane bound Fas ligand ( mFasL ) , soluble Fas ligand ( sFasL ) and matrilysin , the metalloprotease cleaving mFasL to sFasL ] and sperm parameters . ^^^ |
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| Interacting proteins: P09237 and P48023 |
Pubmed |
SVM Score :0.0 |
| Quantitative real time polymerase chain reaction ( TaqMan RTPCR ) was used to elucidate the effect of UVA and UVB irradiation on the expression of genes coding for TNF alpha , IL 1beta , IL 10 , FasL , matrilysin , TACE and furin in HaCaT cells over a 48 h period ( IL 1beta , interleukin 1beta ; FasL , Fas ligand ) . ^^^ |
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| Interacting proteins: P09237 and P48023 |
Pubmed |
SVM Score :0.0 |
| Matrix metalloproteinase 7 ( MMP 7 ) generates soluble Fas Ligand ( FasL ) , which is involved in the apoptotic loss of CD4+ T cells during HIV infection . ^^^ |
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| Interacting proteins: P09237 and P48023 |
Pubmed |
SVM Score :0.0 |
| The ability of TCAH to cleave FasL and thereby decrease AICD appeared to be mediated by metalloproteinases and correlated with a TCAH induced increase in matrix metalloproteinase 7 . ^^^ |
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| Interacting proteins: P09237 and P48023 |
Pubmed |
SVM Score :0.0 |
| In a mouse model of pancreatic acinar to ductal metaplasia , MMP 7 progressively accumulates during the metaplastic transition , resulting in a concomitant increase in solubilization of Fas ligand ( FasL ) . ^^^ Under identical conditions , mice either deficient in MMP 7 or carrying an inactive FasL gene are severely inhibited in development of progressive metaplasia and acinar cell apoptosis . ^^^ Thus , MMP 7 and FasL influence the initiation and maintenance of metaplastic events in pancreatic epithelium , explaining the observed link between metaplasia and apoptosis in pancreas and other gastrointestinal tissues . . ^^^ |
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| Interacting proteins: P09237 and P48023 |
Pubmed |
SVM Score :0.0 |
| In contrast , enhanced FasL shedding , by recombinant MMP 7 , completely protected neurons from Abeta neurotoxicity . ^^^ |
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| Interacting proteins: P09237 and P48023 |
Pubmed |
SVM Score :0.0 |
| CONCLUSIONS : The results suggest that : ( 1 ) upregulation of mesangial FasL may contribute to the glomerular inflammation in proliferative lupus nephritis in vivo ; ( 2 ) proinflammatory cytokines , in particular IL1beta , produced in nephritis can upregulate FasL via the transcription factor NFkappaB in HMC ; and ( 3 ) MMP 7 mediated release of soluble FasL could control the mesangial inflammation . . ^^^ |
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| Interacting proteins: P09237 and P48023 |
Pubmed |
SVM Score :0.0 |
| In the present study , we first analyzed the influence of this metalloproteinase on Fas signaling in SW 480 , HCT 15 and HT 29 colorectal carcinoma ( CRC ) cells by assessing their responses to either an agonistic Fas antibody ( CH 11 ) or the FasL bearing Jurkat cells after they were pretreated with MMP 7 . ^^^ These results suggest that Fas downregulation and a consequential increased resistance to FasL triggered apoptosis resulting from upregulated MMP 7 in colorectal cancer cells could be a key mechanism for their escape from the immune surveillance , thereby predicting a poor survival in CRC patients . . ^^^ |
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| Interacting proteins: P09237 and P48023 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P09237 and P48023 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P09237 and P48023 |
Pubmed |
SVM Score :0.0 |
| NA |
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