| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| The cloned organic anion transporting protein ( Oatp 1 ) can mediate Na ( + ) independent transport of bile salts , sulfobromophthalein , estrogen conjugates , and a variety of other amphipathic cholephilic compounds . ^^^ |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| The oatp 1 stimulated LTC 4 and DNP SG uptake was independent of the Na+ gradient , cis inhibited by known substrates of this transport protein and by 1 mM GSH , and was saturable , with apparent Km values of 0 . 27 + / 0 . 06 and 408 + / 95 microM , respectively . ^^^ |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| The uptake of BQ 123 by COS 7 cells transfected with either Na ( + ) dependent taurocholate cotransporting polypeptide ( Ntcp ) or Na ( + ) independent basolateral organic anion transporting polypeptide ( oatp 1 ) was minimal . ^^^ |
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| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| In choroid plexus epithelial cells , Oatp 2 could be localized to the basolateral cell pole , whereas the first member of the Oatp gene family of membrane transporters to be cloned ( Oatp 1 ) co localized with the alpha ( 1 ) subunit of Na , K ATPase at the apical plasma membrane domain . ^^^ |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| Characterization of the transport properties of organic anion transporting polypeptide 1 ( oatp 1 ) and Na ( + ) / taurocholate cotransporting polypeptide ( Ntcp ) : comparative studies on the inhibitory effect of their possible substrates in hepatocytes and cDNA transfected COS 7 cells . ^^^ In addition , the Na ( + ) independent uptake of E ( 2 ) 17betaG was inhibited by 15 organic anions in a concentration dependent manner , and their inhibitory effects were similar between primary cultured rat hepatocytes and oatp 1 transfected COS 7 cells . ^^^ BQ 123 ( 1 microM ) , pravastatin ( 1 microM ) , and indomethacin ( 10 microM ) , all of which do not undergo oatp 1 mediated transport , significantly inhibited the Na ( + ) independent uptake of E ( 2 ) 17betaG mediated by oatp 1 . ^^^ |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| In the present study , the inducible nature of MRP 3 was examined , using Northern and Western blot analyses , in comparison with that of other secondary active [ Na ( + ) taurocholic acid cotransporting polypeptide ( Ntcp ) , organic anion transporting polypeptide 1 ( oatp 1 ) , and organic cation transporter ( OCT 1 ) ] and primary active [ P glycoprotein ( P gp ) , cMOAT / MRP2 , and MRP 6 ] transporters . alpha Naphthylisothiocyanate treatment and common bile duct ligation induced expression of P gp and MRP 3 , whereas expression of Ntcp , oatp 1 , and OCT 1 was reduced by the same treatment . ^^^ |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| We characterized expression and activity of the bile salt transporters Na ( + ) / taurocholate ( TC ) cotransporting polypeptide ( Ntcp ) , and bile salt export pump ( Bsep ) , and the expression of organic anion transporting polypeptides 1 and 2 ( Oatp 1 and 2 ) and multidrug resistance associated protein 2 ( Mrp 2 ) in pregnancy and throughout lactation in rats . ^^^ |
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| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| These organic anion transporters include ( 1 ) indirectly coupled Na ( + ) / dicarboxylate cotransport and dicarboxylate / organic anion exchange , which is represented on the molecular level by a member of the `` kidney ' ' type organic anion transporter ( OAT ) family at the apical plasma membrane domain ; ( 2 ) the organic anion transporting polypeptide 1 ( Oatp 1 ) and Oatp 2 , which both mediate typical `` liver ' ' like organic anion transport activities at the apical and basolateral plasma membrane domains , respectively ; and ( 3 ) the multidrug resistance protein Mrp1 / MRP1 at the basolateral plasma membrane domain , and the P glycoprotein Mdr1 / MDR1 at an apical and subapical membrane vesicle compartment . ^^^ |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| Here we compare the expression of the basolateral Na ( + ) taurocholate cotransporter ( Ntcp ) and organic anion transporting polypeptides 1 and 2 ( Oatp 1 , Oatp 2 ) and the canalicular bile salt export pump ( Bsep ) and multidrug resistance associated protein 2 ( Mrp 2 ) in primary cultured rat hepatocytes . ^^^ |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| Hepatocellular bile salt uptake is mediated predominantly by the Na ( + ) taurocholate cotransport proteins Ntcp ( rodents ) and NTCP ( humans ) and by the Na ( + ) independent organic anion transporting polypeptides Oatp 1 , Oatp 2 , and Oatp 4 ( rodents ) and OATP C ( humans ) . ^^^ |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| The bile salt uptake system Na ( + ) taurocholate co transporting polypeptide ( Ntcp ) and organic anion transporting polypeptide 1 ( Oatp 1 ) were down regulated by taurocholate and not affected by cholestyramine feeding . ^^^ |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| Impaired sodium dependent uptake of bile acids has been associated with diminished expression of a basolateral Na ( + ) / bile acid cotransporter ( Ntcp ) , whereas sodium independent uptake is maintained despite a down regulation of the organic anion transporter Oatp 1 . ^^^ |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| Upon EE 2 treatment , estrogen receptor alpha ( ERalpha ) repressed the expression of bile acid and cholesterol transporters ( bile salt export pump ( BSEP ) , Na ( + ) / taurocholate cotransporting polypeptide ( NTCP ) , OATP 1 , OATP 2 , ABCG 5 , and ABCG 8 ) in the liver . ^^^ |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| Interaction of Na+ , K ( + ) ATPase from pig kidney in various conformational states with the dialdehyde analogue of ATP , alpha , alpha ( 9 adenyl ) alpha ' D ( hydroxymethyl ) diglycolaldehyde triphosphate ester ( oATP ) , has been studied . ^^^ This interaction leads to an enzyme modification which was shown to be of the affinity type according to the following criteria . 1 . oATP can be hydrolyzed by Na+ , K ( + ) ATPase and prevent inhibition of ATPase activity by gamma [ 4 ( N 2 chloroethyl N methylamino ) ] benzylamide ATP , indicating that it interacts with Na+ , K ( + ) ATPase in the enzyme active site . 2 . oATP irreversibly inhibits ATP hydrolyzing activity of Na+ , K ( + ) ATPase ; the extent of inactivation is decreased in the presence of 20 mM ATP and depends on the ion composition of the modification medium . ^^^ |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| The ( Na+ + Mg2+ ) ATPase of the Acholeplasma laidlawii B plasma membrane was inactivated by the 2 ' , 3 ' dialdehyde derivative of ATP ( oATP ) . oATP behaved as a reversible competitive inhibitor of this ATPase and was slowly hydrolyzed by the enzyme . ^^^ |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| The cloned oatp mediated Na ( + ) independent uptake of sulfobromophthalein ( BSP ) which was Cl ( ) dependent in the presence of bovine serum albumin ( BSA ) at low BSP concentrations ( e . g . , 2 microM ) . ^^^ In addition to BSP , the cloned oatp also mediated Na ( + ) independent uptake of conjugated ( taurocholate ) and unconjugated ( cholate ) bile acids . ^^^ |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| PURPOSE : To examine the expression of multifunctional Na ( + ) independent organic anion transporting polypeptides , termed oatp 1 , oatp 2 , and oatp 3 , involving the transport of thyroid hormone in the rat retina at the protein and mRNA levels . ^^^ |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| We found no differences in hepatic expression of the ATP binding cassette transporter genes Mdr 2 , Bsep ( bile salt export pump ) , Mdr1a / 1b , Abca 1 and Abcg 5 / Abcg 8 ( implicated in cholesterol transport ) , the bile salt uptake systems Ntcp ( Na ( + ) taurocholate co transporting polypeptide gene ) and Oatp 1 ( organic anion transporting polypeptide 1 gene ) or in bile formation between wild type and Ppar alpha ( ( / ) ) mice . ^^^ |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| However , after injection of cRNA of the cloned rat liver Na ( + ) independent organic anion transporting polypeptide oatp , a specific and saturable CRC 220 uptake was observed ( Michaelis Menten constant 29 . 5 mumol / L ) . ^^^ |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| The Na ( + ) independent organic anion transporting polypeptides oatpl ( rat ) and OATP ( human ) exhibit a wide and charge independent substrate specificity . ^^^ |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| Bile acids are taken up by at least two transport systems whose mRNA have been expressed in Xenopus oocytes : ( 1 ) a Na ( + ) dependent system , named NTCP , and ( 2 ) a Na ( + ) independent system , named OATP . ^^^ |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| Bile acids are taken up by at least two transport systems whose mRNAs have been expressed in Xenopus oocytes : a Na ( + ) dependent system , named NTCP , and a Na ( + ) independent system , named OATP . ^^^ |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| Ontogenic expression of the Na ( + ) independent organic anion transporting polypeptide ( oatp ) in rat liver and kidney . ^^^ BACKGROUND / AIMS : A cDNA ( 2 . 7 kb ) encoding a rat liver basolateral Na ( + ) independent organic anion transporter ( oatp ) has recently been cloned . ^^^ |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NTCP mediated Na ( + ) dependent uptake of chlorambucil taurocholate ( Michaelis constant , 11 mumol / L ) , whereas OATP mediated Na ( + ) independent uptake . ^^^ |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| Uptake of [ 3H ] taurocholic acid was saturable and entirely Na+ independent , with the kinetic characteristics of the human liver organic anion transporting polypeptide ( OATP ) . ^^^ OATP , but not the Na+ dependent bile acid transporter ( Na+ taurocholate cotransporting polypeptide [ NTCP ] ) , was detectable by reverse transcription polymerase chain reaction ( RT PCR ) analysis of HepG 2 RNA . ^^^ Therapeutic strategies employing bile acid derived cytostatic agents for the treatment of hepatocellular carcinomas may therefore depend upon the expression of the Na+ independent bile acid transporter OATP in hepatic malignancies . . ^^^ |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| A Na ( + ) dependent bile acid ( Na+ / taurocholate co transporting polypeptide ; Ntcp ) and a Na ( + ) independent bromosulphophthalein ( BSP ) / bile acid uptake system ( organic anion transporting polypeptide ; oatp ) have been cloned from rat liver by using functional expression cloning in Xenopus laevis oocytes . ^^^ In contrast , an oatp specific antisense oligonucleotide when co injected with total rat liver mRNA had no effect on the expression of Na ( + ) dependent taurocholate uptake , but it blocked Na ( + ) independent uptake of taurocholate by approx . 80 % and of BSP by 50 % . ^^^ Assuming similar expression of hepatocellular bile acid and organic anion transporters in Xenopus laevis oocytes , these results indicate that Ntcp and oatp respectively represent the major , if not the only , Na ( + ) dependent and Na ( + ) independent taurocholate uptake systems in rat liver . ^^^ |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| In transfected COS cells , oatp 3 mediated Na ( + ) independent , DIDS inhibited taurocholate uptake ( Michaelis Menten constant approximately 30 microM ) . ^^^ |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| Human OATP E transported 3 , 3 ' , 5 triiodo L thyronine ( K ( m ) , 0 . 9 microM ) , thyronine , and rT ( 3 ) in a Na ( + ) independent manner . ^^^ |
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| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| OATP C ( SLC21A6 ) is the predominant Na ( + ) independent uptake system for bile salts and bilirubin of human liver and is expressed exclusively at the basolateral ( sinusoidal ) hepatocyte membrane . ^^^ |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| The characteristics of this novel skate Oatp , including tissue expression , subcellular localization , substrate selectivity , Na ( + ) dependence , and inhibitor selectivity were generally similar to liver specific human OATP C and rat Oatp 4 . ^^^ |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| Carriers for bile acids [ human Na ( + ) taurocholate cotransporting polypeptide ( NTCP ) ] , organic anions [ organic anion transporting polypeptide ( OATP ) ] , and organic cations [ organic cation transporter ( OCT ) ] were expressed in Xenopus laevis oocytes ( XO ) and Chinese hamster ovary ( CHO ) cells . ^^^ |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| Members of the following transporter families were assessed : three multidrug resistance ( Mdr ) , six multidrug resistance associated protein ( Mrp ) , eight organic anion transporting polypeptide ( Oatp ) , three organic anion transporters ( Oat ) , five organic cation transporters ( Oct ) , two bile acid transporters ( Na ( + ) / taurocholate cotransporting polypeptide and bile salt export protein ) , four metal ( ZnT 1 , divalent metal transporter 1 , Menkes and Wilsons ) , a prostaglandin , two peptide , two sterolin , and four nucleoside transporters . ^^^ |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| The mRNA of members of the organic anion transporting polypeptide ( Oatp ) superfamily , the multidrug resistance protein ( Mrp ) family , one organic anion transporter ( OAT ) , and the bile acid carriers Na ( + ) taurocholate cotransporting polypeptide ( Ntcp ) and bile salt export pump ( Bsep ) was quantified by real time PCR . ^^^ |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| This was within the range of K ( m ) values previously found in human Na ( + ) taurocholate cotransporting polypeptides ( NTCP ) and organic anion transporting polypeptides ( OATP ) 2 and 8 , respectively . ^^^ |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| Among the organic anion transporters , both Na ( + ) taurocholate cotransporting polypeptide ( NTCP ) and various members of the organic anion transporting polypeptide ( OATP ) family mediate transport of iodothyronines . ^^^ |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| The saturable uptake of telmisartan into isolated rat hepatocytes took place in a Na ( + ) independent manner and was inhibited by pravastatin , taurocholate , and digoxin , which are Oatp substrates and inhibitors , but not by organic cation , tetraethylammonium , indicating the involvement of Oatp isoforms in its uptake into rat hepatocytes . ^^^ |
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| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| Uptake of ursodeoxycholate and its conjugates by human hepatocytes : role of Na ( + ) taurocholate cotransporting polypeptide ( NTCP ) , organic anion transporting polypeptide ( OATP ) 1B1 ( OATP C ) , and oatp1B3 ( OATP 8 ) . ^^^ In this study , to check whether three basolateral transporters Na ( + ) taurocholate cotransporting polypeptide ( NTCP , SLC10A1 ) , organic anion transporting polypeptide ( OATP ) 1B1 ( OATP C ) , and OATP1B3 ( OATP 8 ) mediate uptake of UDCA , GUDC , and TUDC by human hepatocytes , we investigated their transport properties using transporter expressing HEK 293 cells and human cryopreserved hepatocytes . ^^^ |
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| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| RT PCR revealed that the organic anion transporting polypeptides OATP B , D , E , and the organic anion transporter OAT 4 are highly expressed , and that OATP A , C , 8 , OAT 3 , and Na ( + ) taurocholate cotransporting polypeptide ( NTCP ) are not or are only lowly expressed in term placental tissue and freshly isolated and cultured trophoblasts . ^^^ |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46721 and Q15599 |
Pubmed |
SVM Score :0.0 |
| NA |
|