| Interacting proteins: P46531 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46531 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46531 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46531 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46531 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46531 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46531 and P19838 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46531 and P19838 |
Pubmed |
SVM Score :0.0 |
| NF kappaB 2 is a putative target gene of activated Notch 1 via RBP Jkappa . ^^^ In transient transfection assays we show that truncated Notch 1 strongly induces NF kappaB 2 promoter activity . ^^^ Moreover , this repression can be overcome by activated Notch 1 , suggesting that NF kappaB 2 is a novel putative Notch target gene . . ^^^ |
|
| Interacting proteins: P46531 and P19838 |
Pubmed |
SVM Score :0.0 |
| Notch 1 regulates NF kappaB activity in hemopoietic progenitor cells . ^^^ We investigated the interaction between two elements critical for differentiation of hemopoietic cells , the Notch 1 receptor and the transcription factor NF kappaB . ^^^ NF kappaB driven transcriptional activity was completely restored after transduction of the cells with retroviral constructs containing activated Notch 1 gene . ^^^ Taken together , these data indicate that in HPC NF kappaB activity is regulated by Notch 1 via transcriptional control of NF kappaB . . ^^^ |
|
| Interacting proteins: P46531 and P19838 |
Pubmed |
SVM Score :0.0 |
| Changes in Notch 1 , Notch 2 , Wnt7b , and Shh expression found in incisor epithelium of Ikk alpha mutants suggest that this NF kappa B independent function is mediated by Notch / Wnt / Shh signaling pathways . . ^^^ |
|
| Interacting proteins: P46531 and P19838 |
Pubmed |
SVM Score :0.0 |
| BxPC 3 cells transfected with Notch 1 cDNA showed induction of NF kappaB activity , and this was inhibited by genistein treatment . ^^^ From these results , we conclude that the inhibition of Notch 1 and NF kappaB activity and their cross talk provides a novel mechanism by which genistein inhibits cell growth and induces apoptotic processes in pancreatic cancer cells . . ^^^ |
|
| Interacting proteins: P46531 and P19838 |
Pubmed |
SVM Score :0.0 |
| Notch 1 augments NF kappaB activity by facilitating its nuclear retention . ^^^ Notch 1 specifically upregulates expression of the cytokine interferon gamma in peripheral T cells through activation of NF kappaB . ^^^ Using biochemical approaches , as well as confocal microscopy , we show that the intracellular domain of Notch 1 ( N1IC ) directly interacts with NF kappaB and competes with IkappaBalpha , leading to retention of NF kappaB in the nucleus . ^^^ |
|
| Interacting proteins: P46531 and P19838 |
Pubmed |
SVM Score :0.0 |
| In our earlier report , we showed that down regulation of Notch 1 reduced nuclear factor kappaB ( NF kappaB ) DNA binding activity and matrix metalloproteinase 9 ( MMP 9 ) expression . ^^^ Because NF kappaB , VEGF , and MMPs are critically involved in the processes of tumor cell invasion and metastasis , we investigated the role and mechanism ( s ) by which Notch 1 down regulation ( using molecular approaches ) may lead to the down regulation of NF kappaB , vascular endothelial growth factor ( VEGF ) , and MMP 9 , thereby inhibiting invasion of pancreatic cancer cells through Matrigel . ^^^ Consistent with these results , we found that the down regulation of Notch 1 reduced NF kappaB DNA binding activity and VEGF expression . ^^^ Taken together , we conclude that the down regulation of Notch 1 could be an effective approach for the down regulation and inactivation of NF kappaB and its target genes , such as MMP 9 and VEGF expression , resulting in the inhibition of invasion and metastasis . . ^^^ |
|
| Interacting proteins: P46531 and P19838 |
Pubmed |
SVM Score :0.0 |
| Using multiple cellular and molecular approaches such as 3 ( 4 , 5 dimethylthiazol 2 yl ) 2 , 5 diphenyltetrazolium bromide assay , apoptosis assay , flow cytometry , gene transfection , real time reverse transcription PCR ( RT PCR ) , Western blotting , and electrophoretic mobility shift assay for measuring DNA binding activity of nuclear factor kappaB ( NF kappaB ) , we found that down regulation of Notch 1 inhibited cell growth and induced apoptosis in pancreatic cancer cells . ^^^ Because Notch 1 is known to cross talk with another major cell growth and apoptotic regulatory pathway ( i . e . , NF kappaB ) , we found that NF kappaB is a downstream target of Notch because down regulation of Notch reduced NF kappaB activity . ^^^ |
|
| Interacting proteins: P46531 and P19838 |
Pubmed |
SVM Score :0.0 |
| It also has been reported that there is cross talk between Notch 1 and another major cell growth and apoptotic regulatory pathway , the nuclear factor kappaB ( NF kappaB ) pathway , which is down regulated by both curcumin and reduction of Notch 1 levels . ^^^ However , to the authors ' knowledge to date , no studies have determined whether the down regulation of Notch 1 signaling , resulting in the inactivation of NF kappaB activity , contributes to curcumin induced cell growth inhibition and apoptosis in pancreatic cancer cells . ^^^ CONCLUSIONS : The current results provide the first demonstration to the authors ' knowledge that the Notch 1 signaling pathway is associated mechanistically with NF kappaB activity during curcumin induced cell growth inhibition and apoptosis of pancreatic cells . ^^^ |
|
| Interacting proteins: P46531 and P19838 |
Pubmed |
SVM Score :0.0 |
| This was accompanied by increased apoptosis and concomitant attenuation of Notch 1 and NF kappaB and down regulation of NF kappaB downstream genes , such as matrix metalloproteinase 9 and vascular endothelial growth factor , resulting in the inhibition of pancreatic cancer cell invasion through the Matrigel . ^^^ Our data suggest that the ERRP mediated inactivation of EGFR , Notch 1 , NF kappaB , and its downstream target genes contributed to the inhibition of cell growth and invasion . ^^^ |
|
| Interacting proteins: P46531 and P19838 |
Pubmed |
SVM Score :0.0 |
| Here we show that TAN 1 has functional properties of an 1 kappa B like regulator with specificity for the NF kappa B p 50 subunit . ^^^ A recombinant polypeptide corresponding to the cytoplasmic portion of TAN 1 ( TAN 1C ) specifically inhibited the DNA binding of p 50 containing NF kappa B complexes . ^^^ Immunoprecipitation experiments showed that the TAN 1 from a T cell line is associated with NF kappa B containing p 50 and p 65 subunits . ^^^ |
|