| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.58033606 |
| The adapter protein CrkL was associated with both phosphorylated Cbl and the guanidine nucleotide releasing factor C3G , which catalyzes guanosine triphosphate ( GTP ) exchange on Rap 1 . 0.58033606^^^ |
|
| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.53730361 |
| Structurally , the amino terminal SH 3 domain of CRKL has been shown to bind proteins such as C3G , SOS , PI 3 K , c ABL or BCR / ABL . 0.53730361^^^ |
|
| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.67753344 |
| The induced association of Crkl with Cbl , Hef 1 , and Fc gamma RI gamma after Fc gamma RI activation and the constitutive association between C3G and Crkl provide the first evidence that a Fc gamma RI gamma Crkl C3G complex may link ITAM receptors to the activation of Rap 1 in myeloid cells . . 0.67753344^^^ |
|
| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.93629932 |
| The guanine exchange factor C3G was found to be associated with CrkL at similar levels before and after RA treatment , but Rapl activation downstream of C3G was not inducible by RA . 0.93629932^^^ |
|
| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.69321984 |
| CrkL was also found to interact , via its N terminal SH 3 domain , with C3G , a guanine exchange factor for the small G protein Rap 1 , which was also rapidly activated in an IL 5 dependent manner in these cells , establishing that CrkL mediates downstream activation of at least two signaling cascades in IL 5 stimulated eosinophils . 0.69321984^^^ |
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| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.52774411 |
| In addition to its interaction with Stat 5 , CrkL interacts with C3G in KT 1 cells and such an interaction regulates the downstream activation of the small GTPase Rap 1 , which also mediates inhibition of cell proliferation . 0.52774411^^^ |
|
| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| CrkL was constitutively associated with a substantial amount of the guanine nucleotide exchange protein C3G , and a fraction of the C3G protein was coimmunoprecipitated with Cbl in activated Jurkat T cells . ^^^ |
|
| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| The binding of two guanine nucleotide exchange factors , Sos and C3G , to Crk and CRKL indicates that Ras or related proteins likely play a role in signaling through Crk family proteins . . ^^^ |
|
| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| The Y207F mutation in CRKL did not enhance or decrease association with various target signalling proteins , including SOS or C3G , which interact specifically with the CRKL N SH 3 domain . ^^^ |
|
| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| In untransformed cells , three major proteins coprecipitated with CRKL , identified as C3G , SOS and c ABL . ^^^ Thus , in these hematopoietic cell lines , CRKL is involved to a greater extent than CRK 2 in normal signaling pathways that involve c ABL , C3G and SOS . ^^^ In BCR / ABL transformed cells , CRKL but not CRK 2 , appears to form complexes which potentially link BCR / ABL , c ABL , C3G , and SOS to the protooncoprotein , p120CBL . . ^^^ |
|
| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| In factor dependent hematopoietic cell lines , CrkL constitutively associated with the guanine nucleotide release factor , C3G , which is known to interact via Crk src homology 3 ( SH 3 ) domains . ^^^ |
|
| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| In both cell types , CRKL is constitutively complexed to C3G , SOS , and c ABL through its SH 3 domains , and the stoichiometry of these complexes does not change upon integrin ligation . ^^^ |
|
| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| Enhancement of guanine nucleotide exchange activity of C3G for Rap 1 by the expression of Crk , CrkL , and Grb 2 . ^^^ C3G also binds to other adaptor proteins , including CrkL and Grb 2 . ^^^ In the present study , we analyzed the effect of Crk , CrkL , and Grb 2 on the C3G Rap 1 pathway . ^^^ Expression of Crk , CrkL , and Grb 2 with C3G in Cos 1 cells significantly increased the ratio of GTP / GDP bound to Rap 1 . ^^^ These results demonstrate that Crk , CrkL , and Grb 2 positively modulate the C3G Rap 1 pathway primarily by recruiting C3G to the cell membrane . . ^^^ |
|
| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| In these cells , CrkL was constitutively complexed with the guanine nucleotide exchange factor C3G , which was found to coimmunoprecipitate with Shc from Epo or IL 3 stimulated cells . ^^^ These results suggest that CrkL is involved in the signaling pathways from the receptors for Epo and IL 3 , most likely by modulating the activity of the Ras family GTPases through its interaction with C3G . . ^^^ |
|
| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| In the IFNalpha sensitive U 266 and Daudi cell lines , CrkL interacts via its N terminus SH 3 domain with the guanine exchange factor C3G that regulates activation of Rap 1 , a small G protein that exhibits tumor suppressor activity . ^^^ Thus , tyrosine phosphorylation of CrkL links the functional Type 1 IFNR complex to the C3G Rap 1 signaling cascade that mediates growth inhibitory responses . . ^^^ |
|
| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| Furthermore , the guanine nucleotide exchange factor C3G , which is thought to be involved in the regulation of the ras pathway and constitutively binds to the C terminal SH 3 domain of CrkL , could be detected in HEF 1 immunoprecipitates . ^^^ Therefore , CrkL is involved in the formation of a HEF 1 CrkL C3G ternary complex in B cells , suggesting that it is likely to play an important role , allowing the propagation of the stimulation initiated by both BCR and beta 1 integrin ligation . . ^^^ |
|
| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| CrkII and CrkL constitutively formed complex with the guanine nucleotide exchange factor C3G , in unstimulated as well as PDGF stimulated cells . ^^^ |
|
| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| Development of highly selective SH 3 binding peptides for Crk and CRKL which disrupt Crk complexes with DOCK 180 , SoS and C3G . ^^^ |
|
| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| In the current study we show that Crkl , but only a minor amount of the related Crk , form constitutive complexes in vivo with guanine nucleotide exchange factor C3G in 3T3 fibroblasts . ^^^ In contrast , 3T3 cells stably transfected with Bcr / Abl P 210 show a prominent reduction in the amount of C3G complexed to Crkl and do not exhibit tyrosine phosphorylation of C3G upon spreading and attachment . ^^^ These data establish that integrin mediated cell adhesion results in Crkl mediated tyrosine phosphorylation of C3G , a pathway which can be disrupted by Bcr / Abl . . ^^^ |
|
| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| CrkL activates integrin mediated hematopoietic cell adhesion through the guanine nucleotide exchange factor C3G . ^^^ Furthermore , overexpression of C3G also increased the adhesion of hematopoietic cells to fibronectin , whereas a C3G mutant lacking the guanine nucleotide exchange domain abrogated the CrkL induced increase in cell adhesion . ^^^ Taken together , these results indicate that the CrkL C3G complex activates VLA 4 and VLA 5 in hematopoietic cells , possibly by activating the small GTP binding proteins , including R Ras , through the guanine nucleotide exchange activity of C3G . . ^^^ |
|
| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| CrkL mediates Ras dependent activation of the Raf / ERK pathway through the guanine nucleotide exchange factor C3G in hematopoietic cells stimulated with erythropoietin or interleukin 3 . ^^^ Overexpression of C3G enhanced the Elk 1 activation synergistically with CrkL , while a C3G mutant lacking the guanine nucleotide exchange domain showed an inhibitory effect . ^^^ Studies using a dominant negative Ha Ras mutant demonstrated that the Elk 1 and ERK 2 activation enhanced by CrkL and C3G was dependent on Ras . ^^^ These data indicate that the CrkL C3G complex plays a role in Epo or IL 3 induced , Ras dependent activation of the Raf / ERK pathway leading to the activation of Elk 1 and the c fos gene transcription . . ^^^ |
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| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| The adapter protein Crkl links Cbl to C3G after integrin ligation and enhances cell migration . ^^^ The major Crkl SH 3 binding protein in these cells was found to be the guanine nucleotide exchange factor , C3G . ^^^ Interestingly , overexpression of C3G also enhanced migration , suggesting that a Cbl Crkl C3G complex may be involved in migration signaling in Ba / F3 cells . ^^^ These data suggest that Crkl is involved in signaling pathways that regulate migration , possibly through a complex with Cbl and C3G . . ^^^ |
|
| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| CrkL then regulates activation of the guanine exchange factor C3G , with which it interacts constitutively via its N terminus src homology 3 domain . ^^^ |
|
| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| CRKL binds via its first SH 3 domain to several downstream signal transducers , including C3G an activator of the small GTPase Rap 1 . ^^^ The results presented here delineate a novel signaling pathway from HGF to the GTPase Rap 1 which depends on the interaction of the adapter protein CRKL with the exchange factor C3G and could be linked to cell migration . . ^^^ |
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| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| The CrkL adaptor protein is involved in signaling from the receptor for erythropoietin ( Epo ) as well as interleukin ( IL ) 3 and activates beta ( 1 ) integrin mediated hematopoietic cell adhesion through its interaction with C3G , a guanine nucleotide exchange factor for Rap 1 . ^^^ The cytokine induced activation of Rap 1 was augmented in cells that inducibly overexpress CrkL or C3G . ^^^ These results suggest that Epo and IL 3 activate Rap 1 at least partly through the CrkL C3G complex as well as through additional pathways most likely involving phospholipase Cgamma and strongly implicate Rap 1 in regulation of beta ( 1 ) integrin mediated hematopoietic cell adhesion . . ^^^ |
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| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| The Crk / CRKL binding region of the Rap 1 specific exchange factor C3G ( CBR ) inhibits the bombesin stimulated Rap 1 activity in transfected Swiss 3T3 cells . ^^^ The CBR is complexed with endogenous c Crk 2 and CRKL and blocks the protein association with catalytically active C3G . ^^^ |
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| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| Western blot analysis showed a 4 6 fold overexpression of transgenic Crkl above endogenous crkl in two lines and increased constitutive complex formation between Crkl and C3G , an exchange factor for the small GTPase Rap 1 . ^^^ |
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| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| Furthermore , GST CD34i ( full ) did not bind c Abl , c Cbl , C3G , or paxillin proteins that are known to associate with CrkL , suggesting that CD 34 directly interacts with the CrkL protein . ^^^ |
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| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| To investigate the molecular basis for Rap 1 activation , we examined complexes containing C3G , a guanine nucleotide exchange factor for Rap 1 , and CrkL , an adapter protein known to influence Rap 1 signaling . ^^^ NGF induced the formation of a long lived complex containing C3G / CrkL / Shp2 / Gab2 / TrkA . ^^^ |
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| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| The cytokine induced activation of Rac was augmented in a 32D / EpoR Wt clone that inducibly overexpresses the adaptor protein CrkL or the Ras guanine nucleotide exchange factor C3G , which forms a complex with CrkL . ^^^ |
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| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| Previous studies have proposed that Cbl is also a positive regulator of CrkL C3G signalling , which leads to Rap 1 activation . ^^^ Remarkably , the membrane translocation of C3G , its association with CrkL , and the guanine nucleotide exchange activity of C3G were all increased in Cbl ( / ) thymocytes . ^^^ |
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| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| Activation of a Dab1 / CrkL / C3G / Rap1 pathway in Reelin stimulated neurons . ^^^ CrkL also binds C3G , an exchange factor ( GEF ) for the small GTPase Rap 1 that is activated in other systems by tyrosine phosphorylation . ^^^ Regulation of Crk / CrkL , C3G , and Rap 1 by Reelin may be involved in coordinating neuron migrations during brain development . . ^^^ |
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| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| Interaction of Bcr / Abl with C3G , an exchange factor for the small GTPase Rap 1 , through the adapter protein Crkl . ^^^ The guanine nucleotide exchange factor for the small GTPase Rap 1 , C3G , binds constitutively to Crkl . ^^^ Here , we report that Crkl mediates the formation of protein complexes that include C3G and Bcr / Abl . ^^^ Co expression of Crkl and C3G with Bcr / Abl generated increased levels of activated Rap 1 . ^^^ |
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| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| A reassembled complex of ARMS / Kidins220 and CrkL , an upstream component of the C3G Rap 1 MAP kinase cascade , is SH 3 dependent . ^^^ However , Tyr ( 1096 ) phosphorylation enables ARMS / Kidins220 to recruit CrkL through its SH 2 domain , thereby freeing the CrkL SH 3 domain to engage C3G for MAP kinase activation in a neurotrophin dependent manner . ^^^ |
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| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| A novel C3G isoform , designated p87C3G , lacking the most amino terminal region of the cognate protein has been found to be overexpressed in two CML cell lines , K 562 and Boff 210 , both expressing Bcr Abl p 210 . p87C3G expression is also highly augmented in patients diagnosed with chronic myeloid leukemia ( CML ) Ph+ , in comparison with healthy individuals , and returns to basal levels after treatment with STI 571 . p87C3G co immunoprecipitates with both CrkL and Bcr Abl in CML cell lines and co immunoprecipitation between p87C3G and Bcr Abl was also detected in primary cells from CML patients . ^^^ |
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| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13905 and P46109 |
Pubmed |
SVM Score :0.0 |
| NA |
|