| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.51332908 |
| In contrast to constitutive Cbl association with Grb 2 , tyrosine phosphorylated Cbl interacts with CrkL via its SH 2 domain only after CD 2 stimulation . 0.51332908^^^ |
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| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| It interacts with SH 2 containing proteins such as phosphatidylinositol 3 ' kinase ( PI3K ) , Grb 2 , Crk 2 , and CrkL . ^^^ |
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| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| As shown , bcr abl activates the ras pathway by phosphorylation of adapter proteins such as Grb 2 and Crkl . ^^^ |
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| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| HGF stimulated Gab 1 association with c Met , Grb 2 , SHP 2 , PI3K , Shc , Crk isoforms and CrkL , but not with PLCgamma 1 . ^^^ |
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| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| To address the relative roles of CRKL and GRB 2 in this system , we compared BCR ABL mutants with defects in binding to one or both adaptors . ^^^ Whereas each single mutant showed a 2 3 fold loss in transforming activity , the double mutant showed a 15 fold reduction , suggesting that GRB 2 and CRKL both contribute to BCR ABL transformation . ^^^ |
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| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| The amount of Cbl associated with CrkL and CrkII exceeded the fraction of Cbl associated with Grb 2 indicating that unlike other receptor systems , the Cbl Crk association represents the dominant complex of Cbl in growth factor stimulated hematopoietic cells . ^^^ |
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| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| Inhibition of Grb 2 and Crkl proteins results in growth inhibition of Philadelphia chromosome positive leukemic cells . ^^^ The Bcr Abl protein has been found to bind to SH2 / SH3 containing adaptor proteins such as Grb 2 and Crkl , and these complexes are believed to activate various signaling pathways . ^^^ Grb 2 and Crkl are important for the Bcr Abl mediated transformation of rat fibroblasts and murine hematopoietic cells . ^^^ We have used liposomes to deliver nuclease resistant antisense oligonucleotides ( oligos ) that are specific for the GRB 2 or CRKL mRNA to leukemic cells to specifically downregulate Grb 2 or Crkl protein expression . ^^^ We found that by downregulating Grb 2 or Crkl protein expression , Grb 2 or Crkl antisense oligos could selectively inhibit the growth of Bcr Abl positive cells , but not that of Bcr Abl negative cells . ^^^ |
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| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| Enhancement of guanine nucleotide exchange activity of C3G for Rap 1 by the expression of Crk , CrkL , and Grb 2 . ^^^ C3G also binds to other adaptor proteins , including CrkL and Grb 2 . ^^^ In the present study , we analyzed the effect of Crk , CrkL , and Grb 2 on the C3G Rap 1 pathway . ^^^ Expression of Crk , CrkL , and Grb 2 with C3G in Cos 1 cells significantly increased the ratio of GTP / GDP bound to Rap 1 . ^^^ These results demonstrate that Crk , CrkL , and Grb 2 positively modulate the C3G Rap 1 pathway primarily by recruiting C3G to the cell membrane . . ^^^ |
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| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| Glutathione S transferase fusion protein data demonstrate that the constitutive interaction of CBL with GRB 2 and CRKL is mediated via two discrete regions of the CBL C terminus . ^^^ |
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| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| In this report we characterize an as yet unidentified 97 kDa protein that is a major tyrosine kinase substrate in IL 2 stimulated cells . pp 97 was found to associate with the p85 . p110 phosphatidylinositol 3 kinase complex , the Src homology 2 ( SH 2 ) domain containing tyrosine phosphatase SHP 2 , and the adaptor molecules CrkL and Grb 2 . ^^^ We demonstrate that these interactions are directly mediated through the SH 2 domains of CrkL , p 85 , and SHP 2 and through the SH 3 domains of Grb 2 . pp 97 was found to mediate the IL 2 induced interaction between p 85 and both a phosphorylated and a non phosphorylated form of SHP 2 . ^^^ |
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| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| The alphaCbl immunoprecipitations revealed constitutive binding of Nck and Grb 2 to Cbl and FcgammaRII inducible binding of CrkL to Cbl . ^^^ |
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| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation of a number of substrates such as GAP , GRB 2 , SHC , FES , CRKL , and paxillin is considered a decisive step in transformation . ^^^ |
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| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| We found that HPK 1 interacted with Crk and CrkL adaptor proteins in vitro and in vivo and that the proline rich motifs within HPK 1 were involved in the differential interaction of HPK 1 with the Crk proteins and Grb 2 . ^^^ |
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| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| The insulin receptor substrate ( IRS ) , SHC , GRB 2 , CRKII and CRKL adaptor proteins have all been implicated in transmitting signals to the nucleus of the cell . ^^^ |
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| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| Cbl is a cytosolic protein that is rapidly tyrosine phosphorylated in response to Fc receptor activation and binds to the adaptor proteins Grb 2 , CrkL , and Nck . ^^^ |
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| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| Our results provide the first evidence that FcalphaR mediated signals modulate a multimolecular adaptor protein complex containing Grb 2 , Shc , SHIP , CrkL , Cbl , and SLP 76 . ^^^ Stimulation of FcalphaRI induced the tyrosine phosphorylation of Shc and increased the association of Grb 2 with Shc and CrkL . ^^^ These data indicate that the stimulation of FcalphaR results in the modulation of adaptor complexes containing tyrosine phosphorylated Cbl , Shc , SHIP , Grb 2 , and Crkl . ^^^ |
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| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| HPK 1 interacts , through its proline rich domains , with growth factor receptor bound 2 ( Grb 2 ) , CT 10 regulated kinase ( Crk ) , and Crk like ( CrkL ) adaptor proteins . ^^^ |
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| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| Supershift analysis was performed with CrkL , Stat 5 , Stat 1 , Grb 2 , and peptide blocked CrkL and Stat 5 antibodies . ^^^ Transcriptional activation was analyzed in COS 7 cells transfected with a Stat responsive luciferase reporter construct and Bcr Abl , kinase defective Bcr Abl , CrkL , or Grb 2 . ^^^ |
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| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| Besides binding motifs for phosphatidylinositol 3 kinase and Grb 2 , Gab 1 contains multiple Tyr 10 10 Pro ( YXXP ) motifs which , when phosphorylated , are potential binding sites for the adapter proteins c Crk and Crk like ( CRKL ) . ^^^ |
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| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| L selectin tyrosine phosphorylates cbl and induces association of tyrosine phosphorylated cbl with crkl and grb 2 . ^^^ There is an activation induced association of tyrosine phosphorylated Cbl with Grb 2 and CrkL , respectively , but not CrkII . ^^^ |
|
| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| Upon TCR / CD3 stimulation , HPK 1 formed inducible complexes with the adapters Nck and Crk with different kinetics , whereas it constitutively interacted with the adapters Grb 2 and CrkL in Jurkat T cells . ^^^ |
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| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| CD 2 crosslinking markedly induced tyrosine phosphorylation of Cbl associated with Grb 2 or CrkL , Shc and LAT , compared with IL 2 stimulation . ^^^ However , costimulation of IL 2 with CD 2 crosslinking remarkably enhanced associations of Grb 2 Shc and CrkL Cbl , compared to IL 2 stimulation or CD 2 crosslinking alone . ^^^ In vitro binding studies using GST fusion proteins revealed that interactions of Grb 2 Shc and CrkL Cbl were mediated through each SH 2 domain in tyrosine phosphorylation dependent manner . ^^^ |
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| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| Overexpression of Gab 2 in K 562 cells resulted in transcriptional activation of the c fos serum response element ( SRE ) promoter , whereas overexpression of SHP 2 , Grb 2 , and CrkL had no effect . ^^^ |
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| Interacting proteins: P46109 and P62993 |
Pubmed |
SVM Score :0.0 |
| The HPK 1 association with Crk , CrkL , and HIP 55 mediate HPK 1 dependent c Jun N terminal kinase ( JNK ) activation , while the association of HPK 1 with SLP 76 , Gads , CrkL , Grb 2 , and Grap affect T and B cell dependent gene transcription . ^^^ |
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