Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P51692 and P46109	Pubmed	SVM Score :0.8829232
Association of CrkL with STAT 5 in hematopoietic cells stimulated by granulocyte macrophage colony stimulating factor or erythropoietin . 0.8829232^^^ Finally , we found that STAT 5 associated with CrkL did not bind to PIE sequence . 0.63013089^^^
Interacting proteins: P51692 and P46109	Pubmed	SVM Score :0.77824117
Interestingly , such association between STAT 5 and Crkl was not observed in cytokine stimulated murine cells , suggesting an intriguing possibility that components of the human STAT 5 DNA complex may be different from those of the murine counterpart . . 0.77824117^^^ Thrombopoietin induces association of Crkl with STAT 5 but not STAT 3 in human platelets . 0.62176768^^^ The coimmunoprecipitaion of Crkl with STAT 5 was inhibited by the immunizing peptide for Crkl antisera or phenyl phosphate ( 20 mmol / L ) . 0.57390841^^^
Interacting proteins: P51692 and P46109	Pubmed	SVM Score :1.4384346
We report that Stat 5 interacts constitutively with the IFN receptor associated Tyk 2 kinase , and during IFNalpha stimulation its tyrosine phosphorylated form acts as a docking site for the SH 2 domain of CrkL . 1.4384346^^^
Interacting proteins: P51692 and P46109	Pubmed	SVM Score :0.68570065
The interaction of CrkL with Stat 5 was facilitated by the function of both the SH 2 and the N terminus SH 3 domains of CrkL . 0.68570065^^^
Interacting proteins: P51692 and P46109	Pubmed	SVM Score :0.0
Crkl , an SH2 / SH3 adapter protein , becomes coimmunoprecipitated specifically with STAT 5 from erythropoietin stimulated erythroid cells ; although it was shown to become associated with c Cbl in the studies using cell lines . ^^^
Interacting proteins: P51692 and P46109	Pubmed	SVM Score :0.0
The adapter protein CrkL is present in DNA bound Stat 5 complexes and predominantly bound to Stat5b . ^^^
Interacting proteins: P51692 and P46109	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Immunoprecipitation of CrkL was performed on lysates from parental cells ( Rat 1 , MO7e , or 32D ) or Bcr Abl expressing cells ( Rat 1p185 , MO7p210 , 32Dp210 , K 562 ) followed by immunoblotting for pTyr , Stat 5 , or CrkL . ^^^ Supershift analysis was performed with CrkL , Stat 5 , Stat 1 , Grb 2 , and peptide blocked CrkL and Stat 5 antibodies . ^^^
Interacting proteins: P51692 and P46109	Pubmed	SVM Score :0.0
Using a beta casein promoter STAT 5 binding site as a probe , we have also demonstrated that CrkL ( a close relative of Crk ) antiserum , but not Crk antiserum , supershifted the STAT 5 DNA complex by an electrophoretic mobility shift assay , suggesting that CrkL , but not Crk , is the major component of the complex . ^^^
Interacting proteins: P51692 and P46109	Pubmed	SVM Score :0.0
In experiments using CrkL ( ) mouse embryonic fibroblasts , we found that CrkL is required for IFN alpha dependent gene transcription via GAS elements , apparently via the formation of DNA binding complexes with Stat 5 . ^^^ On the other hand , gene transcription via ISRE elements is intact in the absence of CrkL , indicating that the regulatory effects on gene transcription are mediated only via the formation of CrkL : Stat 5 complexes . ^^^
Interacting proteins: P51692 and P46109	Pubmed	SVM Score :0.0
K 562 R cells had reduced BCR ABL expression and limited activation of BCR ABL signaling cascades ( Stat 5 , CrkL , MAPK ) . ^^^
Interacting proteins: P51692 and P46109	Pubmed	SVM Score :0.0
In previous studies , we have shown that Stat 5 associates with the CrkL adapter and forms a signaling complex that binds DNA . ^^^
Interacting proteins: P51692 and P46109	Pubmed	SVM Score :0.0
In addition to the well studied IFN induced ISG factor 3 ( ISGF 3 ) and Stat 1 : 1 complexes , IFNs induce the formation of a number of other Stat containing complexes , including Stat 3 : 3 and Stat 5 : 5 homodimers , as well as Stat 2 : 1 and Stat 5 : CrkL heterodimers , that also mediate gene transcription . ^^^
Interacting proteins: P51692 and P46109	Pubmed	SVM Score :0.0
Impaired Crkl expression contributes to the defective DNA binding of Stat5b in nonobese diabetic mice . ^^^ To our surprise , the binding ability of Stat5b is inconsistent with the presence or absence of the Stat5b mutation in these congenic mice but is correlated with the expression levels of the Crkl protein , which was coprecipitated by an anti Stat5b antibody . ^^^ Both the expression of Crkl and the Stat5b binding ability are the highest in B6 . ^^^ We demonstrated that the adapter molecule Crkl can bind Stat5b and that the Crkl protein is a Stat5b binding cofactor . ^^^ More importantly , profection of Crkl recombinant protein significantly increased Stat5b binding ability and rescued the binding defect of the NOD mutant Stat5b , suggesting that Crkl is a key regulatory molecule for Stat5b binding . ^^^
Interacting proteins: P51692 and P46109	Pubmed	SVM Score :0.0
We determined whether IL 5 induces activation of CrkL and STAT 5 in eosinophils using both the human eosinophil differentiated AML14 . 3D10 cell line and purified peripheral blood eosinophils from normal donors . ^^^ Stimulation of AML14 . 3D10 cells or blood eosinophils with IL 5 induced rapid tyrosine phosphorylation of the CrkL adapter and STAT 5 and the association of CrkL and STAT 5 in vivo as evidenced by the detection of STAT 5 in anti CrkL immunoprecipitates . ^^^ Thus , the CrkL adapter plays an important role in IL 5 signaling in the eosinophil , acting as a nuclear adapter for STAT 5 and as an upstream regulator of the C3G Rap 1 signaling pathway . . ^^^
Interacting proteins: P51692 and P46109	Pubmed	SVM Score :0.0
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Interacting proteins: P51692 and P46109	Pubmed	SVM Score :0.0
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Interacting proteins: P51692 and P46109	Pubmed	SVM Score :0.0
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Interacting proteins: P51692 and P46109	Pubmed	SVM Score :0.0
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Interacting proteins: P51692 and P46109	Pubmed	SVM Score :0.0
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