| Interacting proteins: P42229 and P46109 |
Pubmed |
SVM Score :0.8829232 |
| Association of CrkL with STAT 5 in hematopoietic cells stimulated by granulocyte macrophage colony stimulating factor or erythropoietin . 0.8829232^^^ Finally , we found that STAT 5 associated with CrkL did not bind to PIE sequence . 0.63013089^^^ |
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| Interacting proteins: P42229 and P46109 |
Pubmed |
SVM Score :0.77824117 |
| Interestingly , such association between STAT 5 and Crkl was not observed in cytokine stimulated murine cells , suggesting an intriguing possibility that components of the human STAT 5 DNA complex may be different from those of the murine counterpart . . 0.77824117^^^ Thrombopoietin induces association of Crkl with STAT 5 but not STAT 3 in human platelets . 0.62176768^^^ The coimmunoprecipitaion of Crkl with STAT 5 was inhibited by the immunizing peptide for Crkl antisera or phenyl phosphate ( 20 mmol / L ) . 0.57390841^^^ |
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| Interacting proteins: P42229 and P46109 |
Pubmed |
SVM Score :1.4384346 |
| We report that Stat 5 interacts constitutively with the IFN receptor associated Tyk 2 kinase , and during IFNalpha stimulation its tyrosine phosphorylated form acts as a docking site for the SH 2 domain of CrkL . 1.4384346^^^ |
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| Interacting proteins: P42229 and P46109 |
Pubmed |
SVM Score :0.68570065 |
| The interaction of CrkL with Stat 5 was facilitated by the function of both the SH 2 and the N terminus SH 3 domains of CrkL . 0.68570065^^^ |
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| Interacting proteins: P42229 and P46109 |
Pubmed |
SVM Score :0.0 |
| The adapter protein CrkL is present in DNA bound Stat 5 complexes and predominantly bound to Stat5b . ^^^ |
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| Interacting proteins: P42229 and P46109 |
Pubmed |
SVM Score :0.0 |
| K 562 R cells had reduced BCR ABL expression and limited activation of BCR ABL signaling cascades ( Stat 5 , CrkL , MAPK ) . ^^^ |
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| Interacting proteins: P42229 and P46109 |
Pubmed |
SVM Score :0.0 |
| In previous studies , we have shown that Stat 5 associates with the CrkL adapter and forms a signaling complex that binds DNA . ^^^ |
|
| Interacting proteins: P42229 and P46109 |
Pubmed |
SVM Score :0.0 |
| Crkl , an SH2 / SH3 adapter protein , becomes coimmunoprecipitated specifically with STAT 5 from erythropoietin stimulated erythroid cells ; although it was shown to become associated with c Cbl in the studies using cell lines . ^^^ |
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| Interacting proteins: P42229 and P46109 |
Pubmed |
SVM Score :0.0 |
| MATERIALS AND METHODS : Immunoprecipitation of CrkL was performed on lysates from parental cells ( Rat 1 , MO7e , or 32D ) or Bcr Abl expressing cells ( Rat 1p185 , MO7p210 , 32Dp210 , K 562 ) followed by immunoblotting for pTyr , Stat 5 , or CrkL . ^^^ Supershift analysis was performed with CrkL , Stat 5 , Stat 1 , Grb 2 , and peptide blocked CrkL and Stat 5 antibodies . ^^^ |
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| Interacting proteins: P42229 and P46109 |
Pubmed |
SVM Score :0.0 |
| We determined whether IL 5 induces activation of CrkL and STAT 5 in eosinophils using both the human eosinophil differentiated AML14 . 3D10 cell line and purified peripheral blood eosinophils from normal donors . ^^^ Stimulation of AML14 . 3D10 cells or blood eosinophils with IL 5 induced rapid tyrosine phosphorylation of the CrkL adapter and STAT 5 and the association of CrkL and STAT 5 in vivo as evidenced by the detection of STAT 5 in anti CrkL immunoprecipitates . ^^^ Thus , the CrkL adapter plays an important role in IL 5 signaling in the eosinophil , acting as a nuclear adapter for STAT 5 and as an upstream regulator of the C3G Rap 1 signaling pathway . . ^^^ |
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| Interacting proteins: P42229 and P46109 |
Pubmed |
SVM Score :0.0 |
| Using a beta casein promoter STAT 5 binding site as a probe , we have also demonstrated that CrkL ( a close relative of Crk ) antiserum , but not Crk antiserum , supershifted the STAT 5 DNA complex by an electrophoretic mobility shift assay , suggesting that CrkL , but not Crk , is the major component of the complex . ^^^ |
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| Interacting proteins: P42229 and P46109 |
Pubmed |
SVM Score :0.0 |
| In experiments using CrkL ( ) mouse embryonic fibroblasts , we found that CrkL is required for IFN alpha dependent gene transcription via GAS elements , apparently via the formation of DNA binding complexes with Stat 5 . ^^^ On the other hand , gene transcription via ISRE elements is intact in the absence of CrkL , indicating that the regulatory effects on gene transcription are mediated only via the formation of CrkL : Stat 5 complexes . ^^^ |
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| Interacting proteins: P42229 and P46109 |
Pubmed |
SVM Score :0.0 |
| In addition to the well studied IFN induced ISG factor 3 ( ISGF 3 ) and Stat 1 : 1 complexes , IFNs induce the formation of a number of other Stat containing complexes , including Stat 3 : 3 and Stat 5 : 5 homodimers , as well as Stat 2 : 1 and Stat 5 : CrkL heterodimers , that also mediate gene transcription . ^^^ |
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| Interacting proteins: P42229 and P46109 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42229 and P46109 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42229 and P46109 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42229 and P46109 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42229 and P46109 |
Pubmed |
SVM Score :0.0 |
| NA |
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