| Interacting proteins: Q13239 and P43405 |
Pubmed |
SVM Score :0.0 |
| We observed that SLAP is expressed in T cells , and upon TCR activation , SLAP interacts with ZAP 70 , Syk , LAT , and TCRzeta chain in Jurkat T cells . ^^^ In transiently transfected COS 7 cells , SLAP forms separate complexes with ZAP 70 , Syk , and LAT through its Src homology 2 domain . ^^^ |
|
| Interacting proteins: Q13239 and P43405 |
Pubmed |
SVM Score :0.0 |
| Expression of SLAP 2 with SYK or ZAP 70 in COS cells or Jurkat T cells causes the degradation of these kinases , and SLAP 2 overexpression in Jurkat T cells reduces the surface expression of CD 3 . ^^^ These results suggest that the mechanism of action of SLAP 2 and the related protein SLAP is to promote c Cbl dependent degradation of the tyrosine kinases SYK and ZAP 70 and down regulation of CD 3 at the cell surface . . ^^^ |
|
| Interacting proteins: Q13239 and P43405 |
Pubmed |
SVM Score :0.0 |
| Cells so exposed or made to overexpress SLAP exhibited reduced Ag stimulated phosphorylation of Syk ( a cognate of Zap 70 ) , linker for activation of T cells , phospholipase Cgamma , and ERK . ^^^ Small interfering RNA directed against SLAP blocked the induction of SLAP and reversed the inhibitory effects of dexamethasone on phosphorylation of Syk , linker for activation of T cells , and phospholipase Cgamma , but not downstream events , which are likely suppressed by up regulation of downstream of tyrosine kinase 1 and MAPK phosphatase 1 . ^^^ |
|