| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| Closely related molecules with overlapping functions , such as the Syk kinases , SYK , and ZAP 70 , and adaptor molecules , SH 2 domain containing leukocyte protein of 76 kDa and B cell linker protein , are expressed in activated NK cells and are all recruited to the center of the cytolytic synapse . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| Furthermore , the phosphorylation of SLP 76 requires Syk activity but is not dependent on Ca+2 mobilization . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| Consistent with this observation , pp 55 selectively binds to isolated SH 2 domains of Lck , Lyn , Src , and Fyn but not to the SH 2 domains of ZAP 70 , Syk , Shc , SLP 76 , Grb 2 , phosphatidylinositol 3 kinase , and c abl in vitro . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| The cross linking of Ig beta on proB cells freshly isolated from bone marrow of recombination activating gene ( RAG ) 2 deficient mice induced a rapid and transient tyrosine phosphorylation of Ig alpha as well as an array of intracellular proteins including Syk , PI 3 kinase , Vav , and SLP 76 . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| By immunoprecipitation , immunoblotting and / or protease digestion this 72 kDa protein was different from the previously identified 68 76 kDa tyrosine phosphorylated proteins Btk , paxillin , SLP 76 or Syk . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| The KS chimera and downstream ZAP 70 or Syk substrates , such as SLP 76 , were still phosphorylated when expressed in Lck deficient JCaM1 . 6 T cells . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| TCR ligation with either the agonist , weak agonist , or antagonist peptide variants resulted in hyperphosphorylation of CD3zeta , CD3epsilon , ZAP 70 , Syk , Vav , SLP 76 , and pp 36 38 . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| The adaptor protein SLP 76 is expressed in T lymphocytes and myeloid cells and is a substrate for ZAP 70 and Syk . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| Tyrosine phosphorylation of SLP 76 is downstream of Syk following stimulation of the collagen receptor in platelets . ^^^ CRP stimulation of Syk deficient platelets demonstrated that in vivo tyrosine phosphorylation of SLP 76 is downstream of Syk . ^^^ The absence of Syk in the SLP 76 immunoprecipitates raises the possibility that another protein is responsible for bringing SLP 76 to Syk . ^^^ This work identifies SLP 76 as an important adapter molecule that is regulated by Syk and lies upstream of SLAP 130 and PLC gamma 2 in CRP stimulated platelets . . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| SLP 76 is an adapter protein expressed in T cells and myeloid cells that is a substrate for ZAP 70 and Syk . ^^^ Tyrosine phosphorylation of phospholipase C gamma 1 ( but not of Syk ) and calcium mobilization in response to IgE cross linking were reduced in SLP 76 deficient BMMCs . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| Protein tyrosine kinases p 56 ( Lck ) , SYK , and ZAP 70 and downstream adaptors LAT and SLP 76 have been implicated as essential components in T cell activation . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| Evidence that phospholipase C gamma 2 interacts with SLP 76 , Syk , Lyn , LAT and the Fc receptor gamma chain after stimulation of the collagen receptor glycoprotein 6 in human platelets . ^^^ These were found to include PLC gamma 2 , Syk , SH 2 domain containing leucocyte protein of 76 kDa ( SLP 76 ) , Lyn , linker for activation of T cells ( LAT ) and the FcR gamma chain . ^^^ These data indicate that the C terminal SH 2 domain of PLC gamma 2 is important for PLC gamma 2 regulation through possible interactions with SLP 76 , Syk , Lyn , LAT and the FcR gamma chain . . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| Optimal activation of NF kappaB following bpV treatment necessitated downstream effectors of p 56 ( lck ) such as the syk family protein tyrosine kinase ZAP 70 and the molecular adaptor SLP 76 . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| SLP 76 ( / ) bone marrow derived macrophages display FcgammaR mediated tyrosine phosphorylation of Syk , phospholipase C gamma 2 , and extracellular signal regulated kinases 1 and 2 , and normal FcgammaR dependent phagocytosis . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| Importantly , CD 3 induced responses , such as tyrosine phosphorylation of cellular substrates ( LAT , SLP 76 , and PLC gamma 1 ) , as well as calcium mobilization , which are defective in T cells expressing neither ZAP 70 nor Syk , are observed in Syk ( hi ) / ZAP 70 ( ) T cells . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| Expression of SH 2 domain containing leukocyte specific phosphoprotein of 76 kDa ( SLP 76 ) , a hematopoietic cell specific adapter protein , is required to couple Syk family tyrosine kinase activation to downstream mediators such as phospholipase C ( PLC ) gamma following TCR , platelet collagen receptor and mast cell Fc epsilon R stimulation . ^^^ These data show that , although SLP 76 is required to couple Syk family PTK activity to downstream mediators and effector functions in Fc gamma R induced pathways in some cell types , activation of Fc gamma R dependent pathways occurs independently of SLP 76 in BM . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| Whereas tyrosine phosphorylation of Fc ( epsilon ) RI , Syk , and Vav was intact in LAT deficient BMMCs following Fc ( epsilon ) RI engagement , tyrosine phosphorylation of SLP 76 , PLC gamma 1 , and PLC gamma 2 and calcium mobilization were dramatically reduced . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| Lyn , Syk , and SLP 76 , along with tyrosine phosphorylated 28 , 32 , and 130 kDa proteins , bound selectively to a glutathione S transferase protein encoding the SH 2 domains of SHP 1 , suggesting that this is the major site of interaction . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| The increase in phosphorylation of the FcR gamma chain is associated with marked increases in tyrosine phosphorylation of downstream proteins including Syk , linker for activation of T cells ( LAT ) , SLP 76 , and PLCgamma 2 . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| LAT tyrosine phosphorylation is abolished in CRP stimulated Syk deficient mouse platelets , whereas it is not altered in SLP 76 deficient mice or Btk deficient 10 linked agammaglobulinemia ( XLA ) human platelets . ^^^ Using mice engineered to lack the adapter LAT , we showed that tyrosine phosphorylation of Syk and Btk in response to CRP was maintained in LAT deficient platelets whereas phosphorylation of SLP 76 was slightly impaired . ^^^ These results demonstrate that LAT tyrosine phosphorylation occurs downstream of Syk and is independent of the adapter SLP 76 , and they establish a major role for LAT in the phosphorylation and activation of PLCgamma 2 , leading to downstream responses such as alpha granule secretion and activation of integrin alpha ( IIb ) beta ( 3 ) . ^^^ We propose a model in which LAT and SLP 76 are required for PLCgamma 2 phosphorylation but are regulated through independent pathways downstream of Syk . . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| GPVI signals through a pathway that shares many features with those of immune receptors , with critical roles for Syk and the adapters LAT and SLP 76 in the activation of PLCgamma 2 . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| This differs from mice lacking the tyrosine kinase Syk that is upstream of BLNK in BCR signaling and contrasts with mice lacking SLP 76 that is the equivalent adaptor molecule in TCR signal transduction . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| In the present study , we have shown that cross linking of GPVI by JAQ 1 results in tyrosine phosphorylation of the same profile of proteins as that induced by collagen , including the Fc receptor ( FcR ) gamma chain , Syk , LAT , SLP 76 , and phospholipase C gamma 2 . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| In this study , it was discovered that 4N1 1 or its derivative peptide , 4N1K , induces rapid phosphorylation of the Fc receptor ( FcR ) gamma chain , Syk , SLP 76 , and phospholipase C gamma 2 in human platelets . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| Inhibition of Src kinases blocked Syk activation and inhibited phosphorylation of Syk substrates ( Vav 1 , Vav 3 , SLP 76 ) implicated in cytoskeletal regulation . ^^^ Syk deficient platelets exhibited Src activation upon adhesion to fibrinogen , but no spreading or phosphorylation of Vav 1 , Vav 3 , and SLP 76 . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| The platelet collagen receptor glycoprotein 6 ( GPVI ) and the fibrinogen receptor integrin alphaIIbbeta 3 trigger intracellular signalling cascades involving the tyrosine kinase Syk , the adapter SLP 76 and phospholipase Cgamma 2 ( PLCgamma 2 ) . ^^^ In sharp contrast , although fibrinogen binding to platelets stimulates alphaIIbbeta 3 dependent activation of Syk and tyrosine phosphorylation of SLP 76 and PLCgamma 2 , it does not utilize GEMs to promote these responses or to support platelet aggregation . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| Immobilized ophioluxin was used to isolate the glycoprotein 6 Fcgamma complex from resting platelets , which also contained Fyn , Lyn , Syk , LAT , and SLP 76 . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| Using Jurkat cell lines deficient in ZAP70 / Syk ( P 116 ) or LAT ( ANJ 3 ) expression , CD 2 dependent PLCgamma 1 and SLP 76 tyrosine phosphorylation required expression both of ZAP 70 or Syk and of LAT . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| In the Syk Y342F expressing cells there was dramatically impaired receptor induced phosphorylation of multiple signaling molecules , including LAT , SLP 76 , phospholipase C gamma 2 , but not Vav . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| Regulation of blood and lymphatic vascular separation by signaling proteins SLP 76 and Syk . ^^^ Here we identify a failure to separate emerging lymphatic vessels from blood vessels in mice lacking the hematopoietic signaling protein SLP 76 or Syk . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| The same events do not occur in platelet suspensions . alpha2beta1 activation of adherent platelets stimulates tyrosine phosphorylation of many of the proteins in the GPVI FcR gamma chain cascade , including Src , Syk , SLP 76 , and PLCgamma 2 as well as plasma membrane calcium ATPase and focal adhesion kinase . alpha2beta1 mediated spreading is dramatically inhibited in the presence of the Src kinase inhibitor PP 2 and in PLCgamma 2 deficient platelets . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| Significant attenuation of CRP induced tyrosine phosphorylation in known PI3K effectors such as Btk , Tec , PKB / Akt , and phospholipase Cgamma 2 were observed with p85alpha / platelets , whereas no alteration was noted in upstream molecules of Syk , LAT , and SLP 76 . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| In contrast EGCG stimulated tyrosine phosphorylation of platelet proteins , including Syk and SLP 76 but inhibited phosphorylation of focal adhesion kinase . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| With the involvement of Syk , SLP 76 , and Btk , alphaIIbbeta 3 induced PLCgamma 2 activation partly overlaps with the pathway used by the collagen receptor glycoprotein 6 . ^^^ |
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| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| In the present study , we demonstrate that LSARLAF stimulates Src kinase dependent tyrosine phosphorylation of many of the proteins in the GPIIb IIIa cascade , including the tyrosine kinase Syk , the adapter SLP 76 ( SH 2 containing leucocyte phosphoprotein of 76 kDa ) and PLCgamma 2 ( phospholipase Cgamma 2 ) . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| Immunoprecipitation studies indicated that neither collagen alone nor the combination of collagen plus U 46619 induced phosphorylation of the GPVI regulated proteins Syk and SLP 76 ( Src homology 2 containing leucocyte protein of 76 kDa ) . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| To investigate additional functions of the T cell adaptor , Src homology 2 ( SH 2 ) domain containing leukocyte protein of 76 kD ( SLP 76 ) , we performed a yeast two hybrid assay using the N terminal region of SLP 76 fused with the kinase domain of Syk . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| The phosphatidylinositol 3 kinase ( PI3K ) selective inhibitor wortmannin , and mouse platelets deficient in Lyn , Src , Syk , Src homology 2 ( SH 2 ) domain containing leukocyte protein 76 ( SLP 76 ) , phospholipase Cgamma 2 ( PLCgamma 2 ) , linker for activation of T cells ( LAT ) , or Fc receptor gamma chain ( FcRgamma chain ) were used for these studies . ^^^ The results also clearly demonstrate that bt / VWF mediated agglutination induced TxA 2 production is dependent on signaling apparently initiated by Lyn , enhanced by Src , and propagated through Syk , SLP 76 , PI3K , PLCgamma 2 , and protein kinase C ( PKC ) . . ^^^ |
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| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| Following T cell receptor ligation , PLCgamma 1 interacts through its SH 2 and SH 3 domains with the adaptors LAT and SLP 76 , respectively , to form a multiprotein signaling complex that leads to activation of PLCgamma 1 by Syk tyrosine kinases . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| Further , activation of murine platelets by rhodocytin is abolished in the absence of Syk and PLCgamma 2 , and partially reduced in the absence of LAT , SLP 76 , and Vav1 / Vav3 . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| Upon FcepsilonRI cross linking , SLP 76 translocates to the cell membrane , forming clusters that colocalize with the FcepsilonRI , the tyrosine kinase Syk , the adaptor LAT , and phosphotyrosine . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| The signaling that results in TxA 2 production was shown to be initiated by Lyn , enhanced by Src , and propagated through Syk , SLP 76 , PI3K , PLCgamma 2 , and PKC . ^^^ The results demonstrate that Btk is downstream of Lyn , Syk , SLP 76 , and PI3K ; upstream of ERK1 / 2 , PLCgamma 2 , and PKC ; and greatly enhances Akt phosphorylation . ^^^ |
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| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| Furthermore , overexpression of PLD 2 or catalytically inactive PLD2K758R enhanced antigen induced phosphorylations of Syk and its downstream targets , the adaptor proteins LAT and SLP 76 , while expression of a PLD 2 siRNA blocked these phosphorylations . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| Syk tyrosine kinase and Src homology 2 ( SH 2 ) domain containing leukocyte specific phosphoprotein of 76 kDa ( SLP 76 ) are signaling mediators activated downstream of immunoreceptor tyrosine based activation motif ( ITAM ) containing immunoreceptors and integrins . ^^^ While the signaling cascades descending from integrins are similar to immunoreceptors , the mechanism of Syk activation and SLP 76 recruitment remains unclear . ^^^ We used an in vivo structure function approach to study the requirements for the domains of Syk and SLP 76 in immunoreceptor and integrin signaling . ^^^ Syk and SLP 76 also are required for initiating and / or maintaining separation between the blood and lymphatic vasculature . ^^^ Therefore , we correlated the signaling requirement of the various domains of Syk and SLP 76 to their requirement in regulating vascular separation . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| Syk and Slp 76 mutant mice reveal a cell autonomous hematopoietic cell contribution to vascular development . ^^^ Mouse embryos lacking the signaling proteins Syk and Slp 76 develop abnormal blood lymphatic endothelial connections . ^^^ Endogenous Syk and Slp 76 , as well as transgenic GFPSlp 76 , are expressed in circulating cells previously proposed to be endothelial precursors , supporting a causal role for these cells . ^^^ |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: Q13094 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|