| Interacting proteins: P14317 and P43405 |
Pubmed |
SVM Score :0.0 |
| The hematopoietic lineage cell specific protein HS 1 was shown to undergo a process of sequential phosphorylation both in vitro and in vivo , which is synergistically mediated by Syk and Src family protein tyrosine kinases and essential for B cell antigen receptor mediated apoptosis . ^^^ |
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| Interacting proteins: P14317 and P43405 |
Pubmed |
SVM Score :0.0 |
| Syk mediated tyrosine phosphorylation is required for the association of hematopoietic lineage cell specific protein 1 with lipid rafts and B cell antigen receptor signalosome complex . ^^^ |
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| Interacting proteins: P14317 and P43405 |
Pubmed |
SVM Score :0.0 |
| The cross linking of hFc gamma RIIIA induced a rapid increase in tyrosine phosphorylation of several proteins , including PLC gamma 1 , Syk , HS 1 , and p21rasGAP associated p 190 and p 60 proteins . ^^^ |
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| Interacting proteins: P14317 and P43405 |
Pubmed |
SVM Score :0.0 |
| Furthermore , at least 25 % of phosphorylated p40 / 42 co immunoprecipitated with Ig alphabeta , but none precipitated with MHC 2 , CD 40 , Fc ( gamma ) RII , Fyn , HS 1 , or Syk , suggesting that this protein complex specifically associates with the Ig alphabeta heterodimer . p40 / 42 did not react with Abs to Ig alpha , Ig beta , mitogen activated protein kinase , or Lnk . ^^^ |
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| Interacting proteins: P14317 and P43405 |
Pubmed |
SVM Score :0.0 |
| Here , we show that BCR associated tyrosine kinases Lyn and Syk synergistically phosphorylate HS 1 , and that Tyr 378 and Tyr 397 of HS 1 are the critical residues for its BCR induced phosphorylation . ^^^ Wild type HS 1 , but not the mutant , localized to the nucleus under the synergy of Lyn and Syk . ^^^ |
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| Interacting proteins: P14317 and P43405 |
Pubmed |
SVM Score :0.0 |
| Hematopoietic lineage cell specific HS 1 protein is converted into a substrate for c Fgr by previous Syk mediated phosphorylation , at site ( s ) that bind to the SH 2 domain of c Fgr [ Ruzzene , M . , Brunati , A . ^^^ Stimulation of c Fgr catalytic activity is also promoted by the full length HS 1 protein , previously tyrosine phosphorylated by Syk , and is accounted for by an increased Vmax while the Km values are unchanged . ^^^ |
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| Interacting proteins: P14317 and P43405 |
Pubmed |
SVM Score :0.0 |
| In two of five such escapees , alterations in either Syk , HS 1 , and / or Lyn were observed . ^^^ |
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| Interacting proteins: P14317 and P43405 |
Pubmed |
SVM Score :0.0 |
| We found that on cross linking of Fc epsilonRI : 1 ) HCMC released histamine ; 2 ) rapid tyrosine phosphorylation of multiple cellular substrates , including Syk , HS 1 , c Cbl , ERK 1 , and ERK 2 , was observed ; 3 ) intracellular Ca2+ and inositol phosphate production were increased within the first minute after Fc epsilonRI cross linking ; and 4 ) genistein , a tyrosine kinase inhibitor , inhibited both protein tyrosine phosphorylation and histamine release in a dose dependent manner . ^^^ |
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| Interacting proteins: P14317 and P43405 |
Pubmed |
SVM Score :0.0 |
| The binding of antigen to the B cell antigen receptor ( BCR ) results in the activation of protein tyrosine kinases ( PTKs ) such as Lyn and Syk , and the phosphorylation of several substrate proteins including HS 1 and SLP 65 . ^^^ |
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| Interacting proteins: P14317 and P43405 |
Pubmed |
SVM Score :0.0 |
| Thrombin induced tyrosine phosphorylation of HS 1 in human platelets is sequentially catalyzed by Syk and Lyn tyrosine kinases and associated with the cellular migration of the protein . ^^^ By means of specific inhibitors ( PP 2 , SU 6656 , and piceatannol ) and phosphopeptide specific antibodies , as well as by coimmunoprecipitation and binding competition experiments , we show that Syk acts as the primary kinase that phosphorylates HS 1 at Tyr 397 and that Syk phosphorylation is required for HS 1 interaction with the Lyn SH 2 domain . ^^^ Upon docking to Syk phosphorylated HS 1 , Lyn catalyzes the secondary phosphorylation of the protein at Tyr 222 . ^^^ HS 1 Tyr phosphorylation does not occur when thrombin induced actin assembly is inhibited by cytochalasin D even under conditions in which Syk and Lyn are still active . ^^^ All together these results indicate that HS 1 Tyr phosphorylation catalyzed by Syk and Lyn plays a crucial role in the translocation of the protein to the membrane and is involved in the cytoskeleton rearrangement triggered by thrombin in human platelets . . ^^^ |
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| Interacting proteins: P14317 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
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