| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.97865534 |
| Syk activity gradually increased to a maximum at 10 30 min following receptor ligation and was found to parallel the association of Syk with the tyrosine phosphorylated Ig alpha and Ig beta subunits of the receptor . 0.97865534^^^ |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.82604552 |
| In addition , we demonstrate that both ZAP 70 and Syk can bind directly to the phosphorylated Ig alpha and Ig beta subunits with affinities comparable to their binding to the TCR CD 3 epsilon subunit . 0.82604552^^^ |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| These findings not only confirm that both SH 2 domains are necessary for Syk binding to tyrosine phosphorylated Ig alpha and Ig beta but indicate that this binding is necessary for Syk ( Y518 / 519 ) phosphorylation after BCR ligation . ^^^ |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| Syk protein tyrosine kinase is regulated by tyrosine phosphorylated Ig alpha / Ig beta immunoreceptor tyrosine activation motif binding and autophosphorylation . ^^^ Syk activation in B cells correlates with Syk tyrosine phosphorylation as well as with Syk SH 2 mediated association with the tyrosine phosphorylated Ig alpha and Ig beta B cell antigen receptor subunits . ^^^ Tyrosine phosphorylated peptide 20 mers representing Ig alpha and Ig beta immunoreceptor tyrosine activation motifs were synthesized and found to stimulate the specific activity of Syk by as much as 10 fold in vitro . ^^^ Our observations suggest the association of Syk tandem SH 2 domains with the tyrosine phosphorylated Ig alpha and / or Ig beta immunoreceptor tyrosine activation motifs in B cells stimulates Syk autophosphorylation leading to Syk enzyme activation . . ^^^ |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| Of the tyrosine kinases transfected ( Lyn , Blk , Hck , Syk , Fyn ) , only Blk was able to phosphorylate and subsequently associate with cotransfected Ig alpha and Ig beta chimeras in vivo . ^^^ |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| Most strikingly , Ig alpha ( p ) 2ITAM and TCR zeta c and CD 3 epsilon ( p ) 2ITAMs exhibit inverse binding preferences for src and syk family kinases . ^^^ |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| For example , while Ig alpha prefers src family kinases over the Syk kinase and binds Shc avidly , CD 3 epsilon prefers Syk over src kinases and does not bind Shc . ^^^ |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| Furthermore , at least 25 % of phosphorylated p40 / 42 co immunoprecipitated with Ig alphabeta , but none precipitated with MHC 2 , CD 40 , Fc ( gamma ) RII , Fyn , HS 1 , or Syk , suggesting that this protein complex specifically associates with the Ig alphabeta heterodimer . p40 / 42 did not react with Abs to Ig alpha , Ig beta , mitogen activated protein kinase , or Lnk . ^^^ |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| IgM , CD 22 , Ig alpha , Ig beta , and Syk were coimmunoprecipitated with CD 19 from detergent lysates of B lymphocytes . ^^^ |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| Semi quantitative RT PCR analysis performed on mononuclear bone marrow cells from the non XLA patient indicated that lambda like , VpreB , Rag 1 , Rag 2 and TdT transcripts expressed during proB cell stages were found at normal levels whereas E2A , CD 10 , Syk , Pax 5 , CD 19 , Ig alpha , Ig beta , VH C mu and 5 kappa C kappa transcripts characteristic of later stages were severely depressed . ^^^ |
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| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| Cross linking of the IgM receptor induces rapid translocation of IgM associated Ig alpha , Lyn , and Syk tyrosine kinases to the membrane skeleton . ^^^ Furthermore , two BCR associated kinases that are known to play critical roles in anti IgM induced B cell signaling , the src family kinase Lyn and the non src family kinase Syk , accumulate in the membrane skeleton shortly after BCR cross linking , when most of IgM and Ig alpha accumulate in this fraction . ^^^ The kinetics of recruitment of the bulk of Ig alpha , Lyn , and Syk into the membrane skeleton appeared to precede the accumulation of their hypertyrosine phosphorylated forms , suggesting that activation of the BCR associated signaling molecules occurs in this fraction . ^^^ |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| The cross linking of Ig beta on proB cells freshly isolated from bone marrow of recombination activating gene ( RAG ) 2 deficient mice induced a rapid and transient tyrosine phosphorylation of Ig alpha as well as an array of intracellular proteins including Syk , PI 3 kinase , Vav , and SLP 76 . ^^^ |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| To further characterize the intracellular mechanisms of BCR mediated antigen presentation , we used two complementary experimental approaches : mutational analysis of the Ig alpha cytoplasmic tail , and overexpression in B cells of dominant negative syk mutants . ^^^ |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| Following B cell antigen receptor ( BCR ) ligation , the cytoplasmic domains of immunoglobulin alpha ( Ig alpha ) and Ig beta recruit Syk to initiate signaling cascades . ^^^ These data demonstrate that the non ITAM tyrosines of Ig alpha couple Syk activation to BLNK dependent pathways . . ^^^ |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| This analysis revealed that coaggregation of signal incompetent receptors prevented Ag induced Ig alpha and Syk phosphorylation , mobilization of Ca ( 2+ ) , and the up regulation of CD 69 mediated by competent receptors . ^^^ |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| IgA Fc receptor ( CD 89 ) activation enables coupling to syk and Btk tyrosine kinase pathways : differential signaling after IFN gamma or phorbol ester stimulation . ^^^ |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| B cell Ag receptor ( BCR ) signaling occurs via tyrosine phosphorylation of CD79a and CD79b ITAMs , leading to recruitment and activation of Lyn and Syk tyrosine kinases and subsequent downstream events . ^^^ In both CD45+ and CD 45 cells , Ag stimulation led to CD79a and CD79b tyrosine phosphorylation as well as Syk tyrosine phosphorylation , recruitment to the receptors , and activation . ^^^ Surprisingly , in view of previous findings implicating Src family kinases in ITAM phosphorylation , the data indicate that Ag induced CD79a and CD79b tyrosine phosphorylation and Syk recruitment and activation can occur in the absence of CD 45 expression and , hence , Src family kinase activation . . ^^^ |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| A mutant form of Syk lacking tyrosine at position 317 exhibits an enhanced ability to interact with a diphosphopeptide modeled on the immunoreceptor tyrosine based activation motif of the CD79a component of the Ag receptor . ^^^ |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| Among the targets of mIg induced tyrosine phosphorylation are the tyrosine kinases encoded by the lyn , blk , fyn , and syk genes , the mIg associated proteins MB 1 and Ig beta , phospholipase C gamma 1 and gamma 2 , as well as many unidentified proteins . ^^^ |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| Antibodies directed against the carboxyl teminus of Syk , but not against the amino terminal SH 2 domain , co immunoprecipitated MB 1 from activated cells , consistent with the binding of Syk through an SH 2 domain phosphotyrosine interaction . . ^^^ |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P11912 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|