| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| Thus , 3BP2 is an important adaptor that may couple activated Zap 70 / Syk to a LAT containing signaling complex involved in TCR mediated gene transcription . . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| Recent studies showed that LAT is a syk substrate and is tyrosine phosphorylated during T cell stimulation . ^^^ Moreover , LAT , like syk and beta 3 , translocates to the cytoskeleton during the late stage of thrombin stimulated irreversible aggregation and not during ADP stimulated reversible aggregation . . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| Protein tyrosine kinases p 56 ( Lck ) , SYK , and ZAP 70 and downstream adaptors LAT and SLP 76 have been implicated as essential components in T cell activation . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| CD 28 ligation induces rapid tyrosine phosphorylation of the linker molecule LAT in the absence of Syk and ZAP 70 tyrosine phosphorylation . ^^^ Previous studies implicated the protein tyrosine kinases ZAP 70 and Syk in the TCR induced tyrosine phosphorylation of LAT . ^^^ Thus , CD 28 ligation appears to induce tyrosine phosphorylation of LAT by mechanisms that are independent of ZAP 70 and Syk . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| We observed that SLAP is expressed in T cells , and upon TCR activation , SLAP interacts with ZAP 70 , Syk , LAT , and TCRzeta chain in Jurkat T cells . ^^^ In transiently transfected COS 7 cells , SLAP forms separate complexes with ZAP 70 , Syk , and LAT through its Src homology 2 domain . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| Evidence that phospholipase C gamma 2 interacts with SLP 76 , Syk , Lyn , LAT and the Fc receptor gamma chain after stimulation of the collagen receptor glycoprotein 6 in human platelets . ^^^ These were found to include PLC gamma 2 , Syk , SH 2 domain containing leucocyte protein of 76 kDa ( SLP 76 ) , Lyn , linker for activation of T cells ( LAT ) and the FcR gamma chain . ^^^ These data indicate that the C terminal SH 2 domain of PLC gamma 2 is important for PLC gamma 2 regulation through possible interactions with SLP 76 , Syk , Lyn , LAT and the FcR gamma chain . . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| LAT tyrosine phosphorylation is abolished in CRP stimulated Syk deficient mouse platelets , whereas it is not altered in SLP 76 deficient mice or Btk deficient 10 linked agammaglobulinemia ( XLA ) human platelets . ^^^ Using mice engineered to lack the adapter LAT , we showed that tyrosine phosphorylation of Syk and Btk in response to CRP was maintained in LAT deficient platelets whereas phosphorylation of SLP 76 was slightly impaired . ^^^ These results demonstrate that LAT tyrosine phosphorylation occurs downstream of Syk and is independent of the adapter SLP 76 , and they establish a major role for LAT in the phosphorylation and activation of PLCgamma 2 , leading to downstream responses such as alpha granule secretion and activation of integrin alpha ( IIb ) beta ( 3 ) . ^^^ We propose a model in which LAT and SLP 76 are required for PLCgamma 2 phosphorylation but are regulated through independent pathways downstream of Syk . . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| Importantly , CD 3 induced responses , such as tyrosine phosphorylation of cellular substrates ( LAT , SLP 76 , and PLC gamma 1 ) , as well as calcium mobilization , which are defective in T cells expressing neither ZAP 70 nor Syk , are observed in Syk ( hi ) / ZAP 70 ( ) T cells . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| Whereas tyrosine phosphorylation of Fc ( epsilon ) RI , Syk , and Vav was intact in LAT deficient BMMCs following Fc ( epsilon ) RI engagement , tyrosine phosphorylation of SLP 76 , PLC gamma 1 , and PLC gamma 2 and calcium mobilization were dramatically reduced . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| The increase in phosphorylation of the FcR gamma chain is associated with marked increases in tyrosine phosphorylation of downstream proteins including Syk , linker for activation of T cells ( LAT ) , SLP 76 , and PLCgamma 2 . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| Signaling via LAT ( linker for T cell activation ) and Syk / ZAP70 is required for ERK activation and NFAT transcriptional activation following CD 2 stimulation . ^^^ Using Jurkat cell lines deficient in ZAP70 / Syk ( P 116 ) or LAT ( ANJ 3 ) expression , CD 2 dependent PLCgamma 1 and SLP 76 tyrosine phosphorylation required expression both of ZAP 70 or Syk and of LAT . ^^^ As predicted , the absence of either LAT or ZAP70 / Syk kinases correlated with a defect in the induction of nuclear factor of activated T cells ( NFAT ) transcriptional activity , activation of the interleukin 2 promoter , and ERK phosphorylation following CD 2 stimulation . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| GPVI signals through a pathway that shares many features with those of immune receptors , with critical roles for Syk and the adapters LAT and SLP 76 in the activation of PLCgamma 2 . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| In the present study , we have shown that cross linking of GPVI by JAQ 1 results in tyrosine phosphorylation of the same profile of proteins as that induced by collagen , including the Fc receptor ( FcR ) gamma chain , Syk , LAT , SLP 76 , and phospholipase C gamma 2 . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| Chimeric Syk with the myristylation sequence was membrane associated , and a small fraction was constitutively colocalized with FcepsilonRI , Lyn , and LAT ( linker for T cell activation ) in the glycolipid enriched microdomains or rafts . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| We show that SLP 76 and BLNK , but not LAT , effectively co operated with Syk and Tec family PTKs to activate NF AT and NF kappaB . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| Vav 1 deficient bone marrow derived mast cells also exhibited reduced degranulation and cytokine production , although tyrosine phosphorylation of FcepsilonRI , Syk , and LAT ( linker for activation of T cells ) was normal . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| This study found that the combination of vWF and botrocetin , by interacting with GPIb , induced tyrosine phosphorylation of Fc receptor gamma chain ( FcR gamma chain ) , Syk , linker for activation of T cells ( LAT ) , and phospholipase C gamma 2 ( PLCgamma 2 ) . ^^^ However , tyrosine phosphorylation of Syk , LAT , and PLCgamma 2 in response to vWF plus botrocetin was significantly suppressed , suggesting that FcR gamma chain mediates activation signals related to GPIb . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| Syk tyrosine phosphorylation and activation , as well as LAT ( linker for activation of T cells ) tyrosine phosphorylation , both induced by FcepsilonRI clustering , were found to be reduced upon MAFA clustering . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| In washed platelets , vWF / ristocetin and vWF / botrocetin stimulate weak tyrosine phosphorylation of the FcR gamma chain , Syk and PLCgamma 2 , but not the adaptor LAT ( linker for activation of T cells ) , which is localized to glycolipid enriched membrane domains . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| Aggregation of TEC 21 on RBL 2H3 cells induced a rapid increase in tyrosine phosphorylation of several substrates including Syk kinase and LAT adaptor , calcium flux , and release of secretory components . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| Immobilized ophioluxin was used to isolate the glycoprotein 6 Fcgamma complex from resting platelets , which also contained Fyn , Lyn , Syk , LAT , and SLP 76 . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| Fc epsilon RI activation of mast cells is thought to involve Lyn and Syk kinases proximal to the receptor and the signaling complex organized by the linker for activation of T cells ( LAT ) . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| New topographical data are integrated with existing knowledge of the biochemistry of FcepsilonRI signaling and of cell shape during signaling to implicate at least two distinct membrane domains in FcepsilonRI signaling . `` Primary signaling domains ' ' , also called osmiophilic patches , are recognized by their dark staining with osmium , adjacency to coated pits ( previously mapped to planar membrane between lamellae ) and by the characteristic presence of receptor , Syk and PLCgamma 2 , but not Lyn . `` Secondary signaling domains ' ' are characterized by the presence of large elliptical linker for activation of T cells ( LAT ) rafts and of PLCgamma 1 ( previously mapped to lamellae ) but not receptor . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| In the Syk Y342F expressing cells there was dramatically impaired receptor induced phosphorylation of multiple signaling molecules , including LAT , SLP 76 , phospholipase C gamma 2 , but not Vav . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| Significant attenuation of CRP induced tyrosine phosphorylation in known PI3K effectors such as Btk , Tec , PKB / Akt , and phospholipase Cgamma 2 were observed with p85alpha / platelets , whereas no alteration was noted in upstream molecules of Syk , LAT , and SLP 76 . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| This includes collagen stimulated tyrosine phosphorylation of the Fc receptor gamma chain , Syk , LAT and phospholipase Cgamma 2 . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| In the absence of alphaIIb / beta3 and fibrinogen , RFY stimulated a rapid tyrosine phosphorylation of a set of proteins , including Syk , linker for activation of T cells ( LAT ) and phospholipase Cgamma 2 . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| Fc epsilon RI induced signaling in the presence of constitutively active Lyn exhibited enhanced phosphorylation of the receptor subunits , Syk , LAT , Gab 2 , phospholipase C ( PLC ) gamma 1 and PLC gamma 2 , and production of phosphatidylinositol 3 , 4 , 5 trisphosphate . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| Inhibition of actin polymerization followed by Thy 1 aggregation resulted in an increased tyrosine phosphorylation of Syk , phospholipase C gamma ( PLC gamma ) , Gab 2 and linker for activation of T cells ( LAT ) adapters , and some other signaling molecules . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| Signalling by mucetin involved rapid tyrosine phosphorylation of a number of proteins including Syk , Src , LAT and PLC gamma 2 . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| The phosphorylation of Syk , LAT , phospholipase gamma1 / 2 , and Srchomology 2 domain containing protein phosphatase 2 was intact , whereas the phosphorylation of SHIP 1 was significantly reduced in the YFY mutant cells . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| Crosslinked receptors and their signaling partners segregate during signaling to multiple , dynamic membrane domains , including a transient Fc ( epsilon ) RI Lyn domain and at least two other distinct domains , one characterized by the presence of receptor , Syk and multiple signaling proteins , but not Lyn ( primary signaling domains ) , and one characterized by the presence of LAT and PLCgamma 1 but not receptor ( secondary signaling domains ) . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| The inhibitory mechanism involves recruitment of tyrosine phosphatase SHP 1 to FcalphaRI and impairment of Syk , LAT , and ERK phosphorylation induced by FcepsilonRI engagement . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| In the presence of IgG , S . sanguis strain 2017 78 caused the tyrosine phosphorylation of FcgammaRIIA 30s following stimulation , which led to the phosphorylation of Syk , LAT , and PLCgamma 2 . ^^^ During the lag phase prior to platelet aggregation , FcgammaRIIA , Syk , LAT , and PLCgamma 2 were each dephosphorylated , but were re phosphorylated as aggregation occurred . ^^^ As aggregation occurred , SHP 1 was dephosphorylated , while FcgammaRIIA , Syk , LAT , and PLCgamma 2 were rephosphorylated in an RGDS sensitive , and therefore alpha ( IIb ) beta ( 3 ) dependent , manner . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| The phosphatidylinositol 3 kinase ( PI3K ) selective inhibitor wortmannin , and mouse platelets deficient in Lyn , Src , Syk , Src homology 2 ( SH 2 ) domain containing leukocyte protein 76 ( SLP 76 ) , phospholipase Cgamma 2 ( PLCgamma 2 ) , linker for activation of T cells ( LAT ) , or Fc receptor gamma chain ( FcRgamma chain ) were used for these studies . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| Following T cell receptor ligation , PLCgamma 1 interacts through its SH 2 and SH 3 domains with the adaptors LAT and SLP 76 , respectively , to form a multiprotein signaling complex that leads to activation of PLCgamma 1 by Syk tyrosine kinases . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| Further , activation of murine platelets by rhodocytin is abolished in the absence of Syk and PLCgamma 2 , and partially reduced in the absence of LAT , SLP 76 , and Vav1 / Vav3 . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| In AA supplemented cells , the FcepsilonRI mediated beta hexosamidase and TNF alpha release , calcium ( Ca ( 2+ ) ) influx , and some protein tyrosine phosphorylations including Syk and linker for activation of T cells ( LAT ) were enhanced , whereas , in MA or PA supplemented cells , they were not changed when compared with cells cultured in control medium . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| Upon FcepsilonRI cross linking , SLP 76 translocates to the cell membrane , forming clusters that colocalize with the FcepsilonRI , the tyrosine kinase Syk , the adaptor LAT , and phosphotyrosine . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| Furthermore , overexpression of PLD 2 or catalytically inactive PLD2K758R enhanced antigen induced phosphorylations of Syk and its downstream targets , the adaptor proteins LAT and SLP 76 , while expression of a PLD 2 siRNA blocked these phosphorylations . ^^^ |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: O43561 and P43405 |
Pubmed |
SVM Score :0.0 |
| NA |
|