| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| The dimanganese ( 2 , 2 ) catalase from Thermus thermophilus , MnCat ( 2 , 2 ) , arginase from rat liver , Arg ( 2 , 2 ) , and several dimanganese ( 2 , 2 ) compounds , LMn2XY2 , which are functional catalase mimics , all possess a pair of coupled Mn ( 2 ) ions in their catalytic sites . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| This study was aimed at an assessment of the role of oxygen derived free radicals in the pathogenesis of L arginine ( Arg ) induced acute pancreatitis in rat , by measuring the levels of malonyl dialdehyde ( MDA ) , glutathione peroxidase ( GPx ) , catalase , and superoxide dismutase ( Mn and Cu , Zn SOD ) in the pancreatic tissue , and evaluating the protective effect of the xanthine oxidase inhibitor allopurinol . ^^^ The catalase and Mn SOD activities were significantly decreased throughout the study , while the GPx activity was significantly reduced at 6 and 12 hr , and the Cu , Zn SOD activity was significantly lower at 12 hr after the Arg injection as compared with the controls . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| The catalase and Mn SOD activities were significantly decreased throughout the study , while the GPx activity was significantly reduced at 6 and 12 h , and the Cu , Zn SOD activity was significantly lower at 12 h after the Arg injection as compared with the controls . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| A mutation ( CCG > CTG [ Arg > Leu ] ) in codon 463 of katG ( catalase peroxidase ) of Mycobacterium tuberculosis has been found in isoniazid ( INH ) resistant strains . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| KatGs exhibit a peroxidase activity of broad specificity and a high catalase activity , which strongly depends on the presence of a distal Trp as part of the conserved amino acid triad Arg Trp His . ^^^ Thus , to elucidate structure function relationships of catalase peroxidases ( for which no crystal structure is available at the moment ) , we performed UV Vis and resonance Raman studies of recombinant wild type KatG from the cyanobacterium SynechocystisPCC 6803 and the distal side variants ( His 123 > Gln , Glu ; Arg 119 > Ala , Asn ; Trp 122 > Phe , Ala ) . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| These are close to amino acids that are important for the binding of heme to catalase , 44 ( Val ) and 72 75 ( Arg , Val , Val , His ) . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| The fourth mutation was a nucleotide transversion altering the His 244 codon ( CAT ) to an Arg codon ( CGT ) in XP8LO , an intermediate subgroup . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Seven of these tumors contained a CGT to TGT mutation ( Arg to Cys ) and 2 contained a CGT to CAT mutation ( Arg to His ) . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Finally , in one American black kindred and in a black kindred from Ghana the mutation involves CGT CAT ; Arg His 28 . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Catalase activity is regulated by c Abl and Arg in the oxidative stress response . ^^^ This work demonstrates that catalase , a major effector of the cellular defense against H2O2 , interacts with c Abl and Arg . ^^^ The results show that H2O2 induced binding of c Abl and Arg to catalase . ^^^ The SH 3 domains of c Abl and Arg bound directly to catalase at a P293FNP site . c Abl and Arg phosphorylated catalase at Tyr 231 and Tyr 386 in vitro and in the response of cells to H2O2 . ^^^ These findings indicate that c Abl and Arg regulate catalase and that this signaling pathway is of importance to apoptosis in the oxidative stress response . . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Recent studies have shown that catalase activity is stimulated by the c Abl and Arg tyrosine kinases . ^^^ The present work demonstrates that mouse cells deficient in both c Abl and Arg exhibit increased catalase stability . ^^^ Significantly , ubiquitination of catalase is dependent on c Abl and Arg mediated phosphorylation of catalase on both Y 231 and Y 386 . ^^^ These findings indicate that , in addition to stimulating catalase activity , c Abl and Arg promote catalase degradation in the oxidative stress response . . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Influence of the unusual covalent adduct on the kinetics and formation of radical intermediates in synechocystis catalase peroxidase : a stopped flow and EPR characterization of the MET 275 , TYR 249 , and ARG 439 variants . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| However , the novel G > A mutation in exon 9 changes the essential amino acid Arg 354 to Cys 354 and may indeed be responsible for the decreased catalase activity . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Desulfinylation ( reduction to cysteine ) is achieved by a novel enzyme named sulfiredoxin . c Abl and Arg nonreceptor protein tyrosine kinases associate with catalase in cells treated with H ( 2 ) O ( 2 ) by mechanisms involving the SH 3 domains of the kinases and the Pro ( 293 ) PheAsnPro motif of catalase and activate catalase by phosphorylating it on Tyr ( 231 ) and Tyr ( 386 ) . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| The clustered tRNA genes , with their respective anticodons , were as follows : Arg ( ACG ) , Pro ( TGG ) , Ala ( TGC ) , Met ( CAT ) , Ile ( CAT ) , Ser ( TGA ) , fMet ( CAT ) , Asp ( GTC ) , and Phe ( GAA ) . . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Cat decreased ( P less than 0 . 05 ) plasma Gly , Thr , Lys , Pro , Val , Ser , Arg , Leu , Trp , Phe , Asn , Tyr , Met , Ile , Cit , and Asp . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Marked zinc activation of ester hydrolysis by a mutation , 67 His ( CAT ) to Arg ( CGT ) , in the active site of human carbonic anhydrase 1 . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Short oligodeoxynucleotides ( oligos ) possessing two tandem Arg codons followed by TGA stop codon were inserted near the 3 ' end of a modified cat gene . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Occurrence of the alpha 1 22 Arg His ( CGT CAT ) spectrin mutation in Tunisia : potential association with severe elliptopoikilocytosis . ^^^ Following polymerase chain reaction ( PCR ) amplification , cloning and sequencing of exon 2 of spectrin alpha gene in the father , we found the G A substitution at position 2 of codon 22 ( CGT CAT ; Arg His ) . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Hereditary pyropoikilocytosis and elliptocytosis in a white French family with the spectrin alpha I / 74 variant related to a CGT to CAT codon change ( Arg to His ) at position 22 of the spectrin alpha 1 domain . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| As in fox and dog inhibitor , the trypsin inhibiting domain of badger inhibitor contains an Arg residue in the reactive site in contrast to a Lys residue in the inhibitors of lion and cat . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Sequence analysis demonstrated that the alpha 1AT M 3 gene differs from the alpha 1AT M 1 ( Val 213 ) gene by a single base substitution ( Glu 376 GAA Asp 376 GAC ) and from the alpha 1AT M 2 gene by a single base substitution ( His 101 CAT Arg 101 CGT ) . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Sequencing of all coding exons of the M 2 gene revealed it was identical to the common M 1 ( Val 213 ) gene except for two bases ( M 1 ( Val 213 ) CGT Arg 101 , M 2 CAT His 101 ; M 1 ( Val 213 ) GAA Glu 376 M2 GAC Asp 376 ) . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| The localization of two synthetic fragments of the C terminal portion of atrial natriuretic factor : Arg 101 Tyr 126 which displays full biological activity and Leu 94 Arg 109 which is completely devoid of biological activity , has been investigated by immunohisto and immunocytochemical methods in the heart of mammals ( rat , mouse , guinea pig , hamster , rabbit , cat , dog , man ) and nonmammalian vertebrates toad ( Bufo marinus ) , frog ( Rana catesbeiana ) , fish ( Cyprinus carpio , Puntius schwanenfeldi , Cichlosoma biocellatum , Carrasius auratus ) , snake ( Python reticulatus ) and hen . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| The trypsin inhibiting domains of cat and lion inhibitors , however , contain a Lys residue in the reactive site in contrast to an Arg residue in the dog inhibitor . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| As in dog inhibitor the trypsin inhibiting domain of fox inhibitor contains an Arg residue in the reactive site in contrast to a Lys residue in the inhibitors of cat and lion . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Thus , it is possible that Arg 381 is one of the catalytic bases previously observed in the pH dependence of k ( cat ) / Km of TPL with L tyrosine [ Kiick , D . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| The other two mutations were identified in exon 8 : one at codon 273 [ CGT ( Arg ) > CAT ( His ) ] , a CpG site , and one at codon 271 , a silent mutation [ GAG ( Glu ) > GAA ( Glu ) ] . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| A further CESD patient was hemizygous for a His 108 > Arg missense mutation ( CAT > CGT ) in combination with a partial deletion of the LAL gene and was affected more severely . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Nucleotide sequence analysis demonstrated the somatic missense mutation at codon 201 ( CGT ( Arg ) CAT ( His ) ) of G alphas gene in tumor tissue but not in its surrounding tissue . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| The nucleotide sequence analyses demonstrated that the codon CGT for 2 Arg was mutated to CAT for His . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Sequencing analysis revealed a single base mutation at codon 273 from CGT to CAT ( Arg > His ) and immunocytochemical studies provided evidence that the p 53 protein was overexpressed in this cell line . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| In this paper , possible changes in Lys , Arg , Asp and Tyr aminopeptidase activities in the cat brain neocortex ( frontal , parietal , area 17 and areas 18 and 19 as a whole ) were examined during two of the first stages of the critical developmental period ( 15 and 30 days postbirth ) . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| A heterozygous mutation of codon 201 of Gs alpha ( GGT [ Arg ] CAT [ His ] ) was observed in a nodule from an adenomatous goiter . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Direct sequencing of the proinsulin gene exon 3 showed a heterozygous point mutation ( CGT > CAT ) resulting in the substitution of Arg > His in position 65 ( corresponding to the AC cleavage site ) in the index case , his mother , and his maternal grandmother . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Sequencing of the corresponding region has confirmed preliminary data indicating that the single base changes CGT ( Arg ) to TGT ( Cys ) or CGT to CAT ( His ) occurred . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Mutations include an AGG > AGT ( Arg > Ser ) transversion at codon 249 in PLC / PRF / 5 and Mahlavu , an AAT > AAA ( Asn > Cys ) transversion at codon 200 in TONG / HCC , an AAG > GAG ( Lys > Glu ) transition at codon 139 in HCC T , a CAT > CGT ( His > Arg ) transition at codon 214 in SC 4 , and a CCC > CTC ( Pro > Leu ) transition at codon 250 in SC 8 . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Three different mutations were found in the exon 3 sequence of CCKBR : His ( CAT ) at aa 207 > His ( CAC ) ( 5 . 4 % ) , Arg ( CGC ) at aa 215 > His ( CAC ) ( 4 . 5 % ) , and Val ( GTG ) at aa 138 > Met ( ATG ) ( 0 . 9 % ) in controls . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| The order for k ( cat ) / Km values of AAP S at the optimal pH ( pH 7 . 5 ) was Lys > Met > Arg > Ala > Leu > Phe > Tyr > Lys Ala MCAs . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Introduction of Arg to replace Asn 189 , which is mapped on the activation loop , results in a mutant with decreased k ( cat ) , possibly as a result of disruption of the interaction between this residue and basic residues in the vicinity . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| However , like OP Tb , the best substrate for OP Tc was Cbz Arg Arg AMC ( Km = 0 . 72 microM , k ( cat ) = 96 s ( 1 ) ) . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Recombinant granzyme K cleaves synthetic thiobenzyl ester substrates after Lys and Arg with k ( cat ) / K ( m ) values of 3 . 7 10 10 ( 4 ) and 4 . 4 10 10 ( 4 ) M ( 1 ) s ( 1 ) , respectively . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| With a 1 , 6 dicarboxylate substrate ( 2 hydroxymuconate ) , the R61A mutation showed no kinetic effects , while the R11A mutation decreased k ( cat ) 88 fold and increased K ( m ) 8 . 6 fold , suggesting both binding and catalytic roles for Arg 11 . ^^^ With 2 hydroxymuconate , the R39A and R39Q mutations decreased k ( cat ) by 125 and 389 fold and increased K ( m ) by 1 . 5 and 2 . 6 fold , respectively , suggesting a largely catalytic role for Arg 39 . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| As compared to wild type stromelysin 3 , the kinetic parameters K ( M ) and k ( cat ) for the degradation of the fluorogenic substrate Dns Pro Leu Ala Leu Trp Ala Arg NH ( 2 ) ( Dns Leu ) by these mutants indicated that the Gln / Leu substitution led to a 4 fold decrease in catalytic efficiency , whereas the mutations Gln / Tyr and Gln / Arg increased this parameter by a factor 10 . ^^^ The values of the specificity factor , calculated as the ratio ( k ( cat ) / K ( M ) ) Dns Cys ( OMeBn ) ) / ( k ( cat ) / K ( M ) ) Dns Leu , were observed to vary from 26 for the wild type stromelysin 3 to 120 for the Gln / Leu mutant and 25 for the Gln / Arg mutant . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| RESULTS : We found four rare missense mutations [ Exon 4 : Gln ( 108 ) > His ( CAG > CAT ) , Exon 14 : Val ( 648 ) > Met ( GTG > ATG ) and Arg ( 674 ) > Cys ( CGC > TGC ) , and Exon 15 : Ile ( 753 ) > Leu ( ATC > CTC ) ] . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Here , we present the clinical and molecular spectrum of somatic mosaicism considering all five patients with mosaic androgen insensitivity syndrome , whom we have identified since 1993 : Patient 1 ( predominantly female , clitoromegaly ) , 172 TTA ( Leu ) / TGA ( Stop ) ; patient 2 ( ambiguous ) , 596 GCC ( Ala ) / ACC ( Thr ) ; patient 3 ( ambiguous ) , 733 CAG ( Gln ) / CAT ( His ) ; patient 4 ( completely female ) , 774 CGC ( Arg ) / TGC ( Cys ) ; and patient 5 ( ambiguous ) , 866 GTG ( Val ) / ATG ( Met ) . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| The transcript of yeast tRNA ( 3 ) ( Arg ) CA 20 mutant was aminoacylated by E . coli ArgRS with a 10 ( 6 ) increase in k ( cat ) / K ( m ) over that for aminoacylation of yeast tRNA ( 3 ) ( Arg ) transcript . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| The major distinguishing kinetic properties invariably associated with enzymes containing an Arg ( 107 ) residue include an inverse dependence of k ( cat ) on viscosity and lower K ( m ( GSH values relative to enzymes with other residues at that position . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Mutation of Arg 423 decreased k ( cat ) by 10 , 000 fold and decreased apparent affinity for fructose 6 P by 126 fold , while the K ( m ) for PPi increased only 4 fold . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| GeneChip p 53 assay demonstrated that a primary tumor sample from one colon cancer case had a heterozygous point mutation of CGT ( Arg ) to CAT ( His ) at codon 273 in exon 8 . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| The order of the K ( cat ) / K ( m ) values of AAP S at the optimal pH was Arg > Arg Arg > Met > Leu > Lys > Phe > Lys Ala > Tyr > Ala MCAs . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| The k ( cat ) value for poly ( Glu , Tyr ) phosphorylation by the Csk double mutant A316R , R318A is 100 fold greater than the k ( cat ) value for the single R318A mutant , suggesting that an Arg positioned at the alternative location fulfills a similar function as in wild type . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Kinetic characterization of a R234K PSE 4 mutant reveals a 50 fold reduction in k ( cat ) / K ( m ) and confirms the importance of Arg 234 for carbenicillinase activity . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Large decreases in k ( cat ) and k ( cat ) / K ( m ) for all of the Arg 87 and Arg 133 variants indicated that these residues are also important , although not essential , for catalysis . ^^^ Large decreases in k ( cat ) and k ( cat ) / K ( m ) were also observed for the variants in which lysine replaced Arg 87 and Arg 133 , suggesting that the bidentate interaction of these residues with CoA or their greater bulk is important for optimal activity . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| In the absence of Cl ( ) , substrate binding affinity ( 1 / K ( m ) ) of and catalytic efficiency ( k ( cat ) / K ( m ) ) for Ang 1 hydrolysis are increased 6 . 9 and 32 fold , respectively , by the Arg ( 1098 ) to Gln substitution , and are similar ( < 2 fold difference ) to the respective wild type C domain catalytic constants in the presence of optimal [ Cl ( ) ] . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| The most active compounds ( as determined by specificity constant k ( cat ) / K ( m ) ) were peptides with the Arg and Lys residues in the position discussed . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| The R101Q mutant shows smaller , but significant increases in the K ( m ) values for isocitrate and Mn ( 2+ ) ; however , the marked decrease in k ( cat ) suggests a role for Arg ( 101 ) in catalysis . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| However , on changing the arginine ( Arg ) at residue 115 of the T 4 deaminase to either a glutamate ( R115E ) or a glutamine ( R115Q ) , the resulting mutant enzymes were active in the absence of dCTP , with each mutant possessing a turnover number or k ( cat ) that is about 15 % that of the wild type deaminase . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Mutations of Arg ( 63 ) to Ala ( 63 ) ( R63A ) and Arg ( 423 ) to Gly ( 423 ) ( R423G ) resulted in enzymes with increased bile salt independent hydrolytic activity against lysophosphatidylcholine , having 6 . 5 and 2 fold higher k ( cat ) values , respectively , in comparison to wild type CEL . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Residues Arg ( 269 ) , Arg ( 272 ) , Lys ( 283 ) , Arg ( 296 ) , Lys ( 298 ) , and Arg ( 299 ) form a subdomain ( locus ) located N terminal to ( above ) the ATP hydrolysis domain ( P loop ) and mutations in these residues led to either inactive Rho or to proteins displaying decreased k ( cat ) for poly ( C ) dependent ATP hydrolysis , increased K ( m ) for ribo ( C ) ( 10 ) activation , and decreased transcription termination efficiencies ( 57 77 % ) compared with wild type Rho . ^^^ Residues Arg ( 347 ) , Lys ( 348 ) , Lys ( 352 ) , and Arg ( 353 ) form a subdomain ( locus ) C terminal to ( below ) the ATP hydrolysis domain , and mutations in these residues also show a decreased k ( cat ) for poly ( C ) dependent ATP hydrolysis , an increased K ( m ) for ribo ( C ) ( 10 ) activation , and a 50 70 % decrease in transcription termination , compared with wild type Rho . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| By replacing three Lys residues and combining the Ala 26 > Arg mutation that significantly enhanced the affinity for RNA , we engineered a SacY CAT variant that should be suitable for NMR study of the complex . . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| In the absence of A 2 domain dissociation from the A 1 : A 2 : A 3 trimer , APC cleavage at Arg 306 alone caused a sevenfold decrease in affinity for FXa , whereas APC cleavages at Arg 306 , Arg 506 , and Arg 679 caused a 70 fold decrease in affinity for FXa and a 10 fold decrease in the k ( cat ) of the prothrombinase complex for prothrombin without any effect on the apparent K ( m ) for prothrombin . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| In the present study , mutation of the p 53 gene was detected in three DMBA induced leukemias as follows : a single base substitution ( CAT to CGT ) at codon 177 ( exon 5 ) , resulting in an amino acid change from Arg to Leu , a CGG to CTG / CGG changed at codon 211 ( exon 6 ) resulting in an amino acid change from His to Arg / His , and a GGG to TGG at codon 242 ( exon 6 ) resulting in an amino acid change from Gly to Trp , respectively . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| The requirement for Arg in the P 1 position appeared obligatory . k ( cat ) / K ( m ) values indicate that , for highest catalytic efficiency , a basic amino acid is also required in the P 2 position , presenting a motif typical of the cleavage site for the kexin family of subtilases . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| With the exception of an Arg ( L 46 ) Met substitution in the more deeply buried sulfonate binding pocket , modification of the active site resulted in reductions in catalytic efficiency ( k ( cat ) / k ( uncat ) ) , ranging between 3 and 23 fold . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Various replacements of Arg ( 362 ) and substitution of Tyr ( 365 ) with Phe resulted in 79 and 34 fold lower k ( cat ) / K ( m ) values , respectively . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| All cases showed a common missense mutation in exon 8 at codon 273 ( CGT > CAT , Arg > His ) , which was DNA contact mutation in the S 10 beta strand . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| The k ( cat ) / K ( m ) values for the hydrolysis of the synthetic dipeptides FA Ala Lys and FA Ala Arg by CPG 70 were 99 and 56 mm ( 1 ) s ( 1 ) , respectively . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Other single nucleotide changes included five single nucleotide polymorphisms , two intron single nucleotide changes , one synonymous mutation . hMLH 1 EXON19 CODON 749 TAC > TAG ( Tyr > 10 ) , hMSH 2 EXON12 CODON 629 CAA > CGA ( Gln > Arg ) and hMSH 2 EXON15 CODON 839 CAT > CGT ( His > Arg ) were new discovered mutations . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| The measurement of the steady state kinetic parameters and arsenate inhibition constants K ( 1 ) of the enzyme and their mutants showed that the k ( cat ) / K ( m ) values of Arg ( 130 ) and Arg ( 158 ) mutants decreased , and K ( 1 ) values increased obviously compared with those of the wild enzyme . ^^^ In addition , the slight difference for the k ( cat ) values between the R ( 130 ) L and R ( 130 ) L / S ( 131 ) A mutants suggested that Arg ( 130 ) mutation disrupted the hydrogen bond between Ser ( 131 ) and Cys ( 124 ) . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| The subtypes of p 53 mutations included 68 . 9 % ( 31 / 45 ) transitions and 15 . 6 % ( 7 / 45 ) transversions . 39 . 5 % ( 15 / 38 ) of point mutations were CGT ( Arg ) to CAT ( His ) mutation at codon 273 of exon 8 . 34 . 2 % ( 13 / 38 ) of point mutations were CGG ( Arg ) to TGG ( Trp ) mutation at codon 248 of exon 7 . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Molecular cloning and sequencing analysis indicated that this new allele differs from HLA A * 1102 by three nucleotide substitutions in exon 3 , 524 A > G , 526 G > C , and 527 C > G , thus changing codon 175 from His to Arg ( CAT > CGT ) and codon 176 from Ala to Arg ( GCG > CGG ) . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| This was shown by a minor reduction in catalytic activity toward an Arg containing substrate ( k ( cat ) reduction of 25 % ) . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Previous steady state kinetic studies of the R160Q human SO mutant showed a remarkable decrease in k ( cat ) / K ( m ) ( sulfite ) of nearly 1000 fold , which suggests that Arg 160 in human SO makes an important contribution to the binding of sulfite near the molybdenum cofactor [ Garrett , R . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Our results show that the missing codon pairs CCU : UAG ( Pro : Stop ) and CCC : UAG ( Pro : Stop ) had decreasing effect , whereas another missing pair CCU : AGG ( Pro : Arg ) had an opposite effect on the yield of CAT protein in comparison with the wild type cat gene . . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| The combination of good kinetic and spectroscopic properties resulted in a > 20 fold improvement in the sensitivity of renin assay , compared with the commercial substrate Arg Glu ( EDANS ) Ile His Pro Phe His Leu Val Ile His Thr Lys ( DABCYL ) Arg [ where EDANS is 5 [ ( 2 aminoethyl ) amino ] naphthalene 1 sulphonic acid and DABCYL is 4 ( 4 dimethylaminophenylazo ) benzoic acid ] ( k ( cat ) / K ( m ) =268000 M ( 1 ) 10 s ( 1 ) , quenching efficiency < 80 % ) . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| After producing and purifying recombinant hK 5 in yeast , we determined the k ( cat ) / K ( m ) ratio of the fluorogenic substrates Gly Pro Arg AMC and Gly Pro Lys AMC , and showed that it has trypsin like activity with strong preference for Arg over Lys in the P 1 position . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| TPP 1 hydrolyzed most effectively the peptide Ala Arg Phe * Nph Arg Leu ( * , cleavage site ) ( k ( cat ) / K ( m ) = 2 . 94 microM ( 1 ) . s ( 1 ) ) . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| The mis sense mutations were Met 452 > Thr in 17 cats ( 35 nodules ) , Ser 504 > Arg ( two different mutational forms ) in two cats ( two nodules ) , Val 508 > Arg in one cat ( three nodules ) , Arg 530 > Gln in one cat ( two nodules ) , Val 557 > Leu in 13 cats ( 36 nodules ) , Thr 631 > Ala or Thr 631 > Phe ( each mutation seen in one nodule of one cat ) , Asp 632 > Tyr in six cats ( 10 nodules ) and Asp 632 > His in one cat ( one nodule ) . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| Relative k ( cat ) was highly sensitive to a His substitution at Arg ( 496 ) but not at Arg ( 495 ) . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| One novel missense variant in exon 4 , derived from the nucleotide transition in codon 162 ( CGT > CAT : 485G > A ) resulting in an arginine to histidine ( Arg > His ) change , was detected in these subjects . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| The Michaelis constants for synthesis of PArg are 0 . 936 and 1 . 290 mM for L arginine and ATP , respectively and k ( cat ) / K ( m ) ( Arg ) 174 . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| One KCNRG missense mutation , CGT CAT ( Arg His ) was found at codon 92 within the T 1 domain . ^^^ |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P04040 and P42684 |
Pubmed |
SVM Score :0.0 |
| NA |
|