| Interacting proteins: P42566 and P00533 |
Pubmed |
SVM Score :0.0 |
| Structural requirements of the epidermal growth factor receptor for tyrosine phosphorylation of eps 8 and eps 15 , substrates lacking Src SH 2 homology domains . ^^^ |
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| Interacting proteins: P42566 and P00533 |
Pubmed |
SVM Score :0.0 |
| Antibodies specific for the eps 15 gene product recognize two proteins : a major species of 142 kDa and a minor component of 155 kDa , both of which are phosphorylated on tyrosine following EGFR activation by EGF in vivo . ^^^ EGFR is also able to directly phosphorylate the eps 15 product in vitro . ^^^ |
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| Interacting proteins: P42566 and P00533 |
Pubmed |
SVM Score :0.0 |
| We isolated Eps 15 , a substrate of the epidermal growth factor receptor ( EGFR ) tyrosine kinase , and Eps15R , a novel protein with high sequence homology to the carboxyl terminal domain of Eps 15 . ^^^ Furthermore , we found that immunoprecipitates of activated EGFR kinase stably bound in vitro translated Eps 15 only in the presence of in vitro translated 5 Crk . ^^^ Crk might therefore be involved in Eps 15 mediated signal transduction through the EGFR . . ^^^ |
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| Interacting proteins: P42566 and P00533 |
Pubmed |
SVM Score :0.0 |
| Employing an expression cloning approach for tyrosine kinase substrates , we have previously isolated the coding sequence for a novel putative EGFR substrate , eps 15 , from NIH3T3 fibroblasts . ^^^ |
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| Interacting proteins: P42566 and P00533 |
Pubmed |
SVM Score :0.0 |
| Mapping of the molecular determinants involved in the interaction between eps 15 and AP 2 . eps 15 , a substrate for the epidermal growth factor receptor and other receptor tyrosine kinases , possesses a discrete domain structure with protein binding properties . ^^^ |
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| Interacting proteins: P42566 and P00533 |
Pubmed |
SVM Score :0.0 |
| Several proteins from diverse organisms have been shown to share a region of sequence homology with the mammalian epidermal growth factor receptor tyrosine kinase substrate Eps 15 . ^^^ |
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| Interacting proteins: P42566 and P00533 |
Pubmed |
SVM Score :0.0 |
| Conversely , expression of Eps 8 , but not of other EGFR substrates such as Shc or Eps 15 , is virtually extinguished in non proliferating , terminally differentiated murine myogenic cells . ^^^ |
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| Interacting proteins: P42566 and P00533 |
Pubmed |
SVM Score :0.0 |
| The recently discovered localization of epidermal growth factor receptor pathway substrate clone 15 ( Eps 15 ) to plasma membrane clathrin coated pits and its constitutive association with the endocytic clathrin adaptor protein complex , AP 2 , strongly suggest that Eps 15 has an important role in the pathway of clathrin dependent endocytic traffic . ^^^ |
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| Interacting proteins: P42566 and P00533 |
Pubmed |
SVM Score :0.0 |
| Eps 15 is a phosphorylation substrate of the epidermal growth factor receptor kinase . ^^^ |
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| Interacting proteins: P42566 and P00533 |
Pubmed |
SVM Score :0.0 |
| Immunoprecipitation / immunoblotting experiments also demonstrated an association between EAST and eps 15 , an EGFR substrate associated with clathrin coated pits and vesicles , which is essential in the endocytotic pathway . ^^^ EAST , an epidermal growth factor receptor and Eps 15 associated protein with Src homology 3 and tyrosine based activation motif domains . ^^^ |
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| Interacting proteins: P42566 and P00533 |
Pubmed |
SVM Score :0.0 |
| It interacts with Eps 15 , another EGFR substrate which is involved in receptor endocytosis . ^^^ |
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| Interacting proteins: P42566 and P00533 |
Pubmed |
SVM Score :0.0 |
| It can be proposed that APs are capable of binding to the endosomal membrane via a mechanism that requires AP interaction with the intracellular tails of multimeric receptors like activated EGFR , which in turn allows recruitment of clathrin and eps 15 . ^^^ |
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| Interacting proteins: P42566 and P00533 |
Pubmed |
SVM Score :0.0 |
| Eps 15 is a substrate for the tyrosine kinase of the epidermal growth factor receptor ( EGFR ) and is characterized by the presence of a novel protein : protein interaction domain , the EH domain . ^^^ In this study we show that , upon activation of the EGFR kinase , eps 15 undergoes dramatic relocalization consisting of 1 ) initial relocalization to the plasma membrane and 2 ) subsequent colocalization with the EGFR in various intracellular compartments of the endocytic pathway , with the notable exclusion of coated vesicles . ^^^ Relocalization of eps 15 is independent of its binding to the EGFR or of binding of the receptor to AP 2 . ^^^ Eps 15 is recruited to the plasma membrane upon epidermal growth factor receptor activation and localizes to components of the endocytic pathway during receptor internalization . ^^^ |
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| Interacting proteins: P42566 and P00533 |
Pubmed |
SVM Score :0.0 |
| Eps 15 was originally identified as a substrate for the kinase activity of the epidermal growth factor receptor ( EGFR ) . ^^^ |
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| Interacting proteins: P42566 and P00533 |
Pubmed |
SVM Score :0.0 |
| We further found that the EGF induced ubiquitination of Eps 15 occurred both in the absence and presence of endocytosis with the same kinetics as polyubiquitination of the EGFR , but that the EGF induced monoubiquitination of Eps 15 was somewhat reduced upon overexpression of mutant dynamin . ^^^ |
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| Interacting proteins: P42566 and P00533 |
Pubmed |
SVM Score :0.0 |
| Eps 15 , an endocytic protein that is tyrosine phosphorylated by EGFR , is a candidate for such a function . ^^^ Here , we show that tyrosine phosphorylation of Eps 15 is necessary for internalization of the EGFR , but not of the TfR . ^^^ A phosphorylation negative mutant of Eps 15 acted as a dominant negative on the internalization of the EGFR , but not of the TfR . ^^^ A phosphopeptide , corresponding to the phosphorylated sequence of Eps 15 , inhibited EGFR endocytosis , suggesting that phosphotyrosine in Eps 15 serves as a docking site for a phosphotyrosine binding protein . ^^^ |
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| Interacting proteins: P42566 and P00533 |
Pubmed |
SVM Score :0.0 |
| Double immunofluorescence and confocal microscopy revealed that activated KGFR , differently from epidermal growth factor receptor ( EGFR ) , did not induce recruitment of eps 15 to the cell plasma membrane . ^^^ Microinjection of a monoclonal antibody directed against the C terminal DPF domain which contains the AP 2 binding region of eps 15 led to inhibition of both pathways of receptor mediated endocytosis , the EGFR ligand induced endocytosis and the transferrin constitutive endocytosis , but did not appear to block the KGFR ligand induced internalization . ^^^ |
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| Interacting proteins: P42566 and P00533 |
Pubmed |
SVM Score :0.0 |
| We have demonstrated previously that c Cbl requires the presence of a functional ubiquitin interacting motif ( UIM ) in Eps 15 to mediate epidermal growth factor receptor ( EGFR ) endocytosis . ^^^ Both the ubiquitin ligase activity of c Cbl and the UIM of Eps 15 were necessary for plasma membrane recruitment of Eps 15 and entry of ligand bound EGFR into coated pits and vesicles containing Eps 15 . ^^^ This is consistent with a scenario in which ubiquitin moieties appended to activated EGFR complexes act as docking sites for Eps 15 and thereby recruit receptors into clathrin coated pits . ^^^ We find that c Cbl can guide ligand bound EGFR into the Eps 15 internalization route by two distinct mechanisms . ^^^ No strict requirement exists for either ubiquitin modified EGFR or the Cbl binding ubiquitination substrate CIN 85 as docking site for the UIM of Eps 15 . ^^^ |
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| Interacting proteins: P42566 and P00533 |
Pubmed |
SVM Score :0.0 |
| Non clathrin internalization of ubiquitinated EGFR depends on its interaction with proteins harboring the Ub interacting motif , as shown through the ablation of three Ub interacting motif containing proteins , eps 15 , eps15R , and epsin . ^^^ |
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| Interacting proteins: P42566 and P00533 |
Pubmed |
SVM Score :0.0 |
| We have identified a regulated interaction between parkin and Eps 15 , an adaptor protein that is involved in epidermal growth factor ( EGF ) receptor ( EGFR ) endocytosis and trafficking . ^^^ Treatment of cells with EGF stimulates parkin binding to both Eps 15 and the EGFR and promotes parkin mediated ubiquitination of Eps 15 . ^^^ We propose that by ubiquitinating Eps 15 , parkin interferes with the ability of the Eps 15 UIMs to bind ubiquitinated EGFR , thereby delaying EGFR internalization and degradation , and promoting PI ( 3 ) K Akt signalling . ^^^ |
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| Interacting proteins: P42566 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42566 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42566 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42566 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42566 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42566 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42566 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P42566 and P00533 |
Pubmed |
SVM Score :0.0 |
| NA |
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