| Interacting proteins: Q6R327 and P42345 |
Pubmed |
SVM Score :0.0 |
| Here , we report that a mammalian counterpart of TORC 2 ( mTORC 2 ) also exists . mTORC 2 contains mTOR , mLST 8 and mAVO 3 , but not raptor . ^^^ |
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| Interacting proteins: Q6R327 and P42345 |
Pubmed |
SVM Score :0.0 |
| Phosphorylation and regulation of Akt / PKB by the rictor mTOR complex . ^^^ We show that in Drosophila and human cells the target of rapamycin ( TOR ) kinase and its associated protein rictor are necessary for Ser 473 phosphorylation and that a reduction in rictor or mammalian TOR ( mTOR ) expression inhibited an Akt / PKB effector . ^^^ The rictor mTOR complex directly phosphorylated Akt / PKB on Ser 473 in vitro and facilitated Thr 308 phosphorylation by PDK 1 . ^^^ Rictor mTOR may serve as a drug target in tumors that have lost the expression of PTEN , a tumor suppressor that opposes Akt / PKB activation . . ^^^ |
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| Interacting proteins: Q6R327 and P42345 |
Pubmed |
SVM Score :0.0 |
| Double stranded DNA dependent protein kinase ( DNA PK ) , ataxia telangiectasia mutated ( ATM ) gene product , integrin linked kinase ( ILK ) , protein kinase Calpha ( PKCalpha ) , and mammalian target of rapamycin ( mTOR ) , when complexed to rapamycin insensitive companion of mTOR ( RICTOR ) , have all been identified as playing a critical role in Akt Ser 473 phosphorylation . ^^^ In vitro kinase assays , using different subcellular fractions of 3T3 L 1 adipocytes , revealed that phosphatidylinositol 3 , 4 , 5 trisphosphate stimulated Ser 473 phosphorylation correlated well with the amount of DNA PK , mTOR , and RICTOR but did not correlate with levels of ATM , ILK , and PKCalpha . ^^^ Vesicles immunoprecipitated from low density microsomes using antibodies directed against mTOR or RICTOR had phosphatidylinositol 3 , 4 , 5 trisphosphate stimulated Ser 473 activity that was sensitive to wortmannin but not staurosporine . ^^^ Based on our cell free kinase and small interference RNA results , we conclude that mTOR complexed to RICTOR is the Ser 473 kinase in 3T3 L 1 adipocytes . . ^^^ |
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| Interacting proteins: Q6R327 and P42345 |
Pubmed |
SVM Score :0.0 |
| The distinct complex of mTOR , mLST 8 , and rictor ( mTORC 2 ) does not interact with FKBP 12 rapamycin and is not thought to be rapamycin sensitive . mTORC 2 phosphorylates and activates Akt / PKB , a key regulator of cell survival . ^^^ |
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| Interacting proteins: Q6R327 and P42345 |
Pubmed |
SVM Score :0.0 |
| RNA interference mediated knockdown of TSC 2 , p 70 S6 kinase ( S6K1 ) , raptor , or rictor demonstrates that mTOR regulates mitochondrial activity independently of its previously identified cellular targets . ^^^ |
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| Interacting proteins: Q6R327 and P42345 |
Pubmed |
SVM Score :0.0 |
| Multiallelic disruption of the rictor gene in mice reveals that mTOR complex 2 is essential for fetal growth and viability . ^^^ |
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