| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.52846846 |
| Pituitary adenylate cyclase activating polypeptide type 1 receptor ( PACAPr ) belongs to the novel subfamily of the G protein coupled receptors with a long extracellular N terminus , which functions as a major binding site for the PACAP . 0.52846846^^^ Pituitary adenylate cyclase activating polypeptide type 1 receptor ( PACAPr ) belongs to the novel subfamily of the G protein coupled receptors with a long extracellular N terminus , which functions as a major binding site for the PACAP . 0.51843732^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| The data suggest that the effects of both peptides are mediated through a common PACAP preferring receptor that is pharmacologically different from the mammalian PACAP type 1 receptor . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| We have addressed the question whether this extracellular amino terminus of the PACAP type 1 receptor can solely function as a PACAP binding domain . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Pharmacological properties of two recombinant splice variants of the PACAP type 1 receptor , transfected and stably expressed in CHO cells . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Chinese hamster ovary ( CHO ) K 1 cells transfected with the cDNA specifically bound PACAP and mediated PACAP triggered intracellular accumulation of cAMP , indicating that this cDNA encoded a functional human PACAP Type 1 receptor . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Messenger RNA analysis indicated that at least three splice variants of the PACAP type 1 receptor may be expressed in 6 / 23 cells . . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| After a brief overview of characterized GPCR splice variants , we will describe in more detail the functional properties of the PACAP type 1 receptor splice variants . ^^^ The PACAP type 1 receptor is therefore one of the few examples in which alternative splicing is clearly linked to functional diversity . . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Mouse PACAPR 3 is a protein of 437 amino acids that has 50 % and 51 % identity with rat PACAP type 1 and type 2 receptors , respectively . ^^^ Expression of recombinant mouse PACAPR 3 in mammalian cells shows that it binds to vasoactive intestinal polypeptide as well as PACAP 38 and 27 , with a slightly higher affinity for PACAP 38 , and is positively coupled to adenylate cyclase . ^^^ The expression of PACAPR 3 in Xenopus oocytes indicates that calcium activated chloride currents are evoked by PACAP and vasoactive intestinal polypeptide , suggesting that PACAPR 3 can also be coupled to phospholipase C . ^^^ These results suggest that the diverse biological effects of PACAP are mediated by a family of structurally related proteins and that PACAPR 3 participates in the regulation of insulin secretion . . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Here we report the molecular cloning and functional expression of the PACAP type 1 receptor isolated from an AR 4 2J cell cDNA library by cross hybridization screening with a rat VIP receptor cDNA . ^^^ Results of binding studies and stimulation of cellular cAMP accumulation in COS 7 cells transfected with this cDNA are characteristic of a PACAP type 1 receptor . ^^^ Cloning of the PACAP type 1 receptor will enhance our understanding of its distribution , structure , and functional properties and ultimately increase our understanding of its physiological role . . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| The order of potency of these ligands was PACAP 38 PACAP 27 > VIP > PHI , suggesting that the receptor corresponds to the pharmacologically characterised PACAP Type 1 receptor . . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| The PACAP type 1 receptor ( found in hypothalamus , brain stem , pituitary , adrenal gland and testes ) , specific for PACAP , is positively coupled to adenylyl cyclase and phospholipase C . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Pituitary adenylate cyclase activating polypeptide ( PACAP ) analogues were tested for their ability to occupy the recombinant selective PACAP receptors ( PACAP type 1 receptor ) or the non selective PACAP vasoactive intestinal polypeptide ( VIP ) receptors ( PACAP type 2 , VIP 1 and VIP 2 receptors ) stably transfected and expressed in Chinese hamster ovary ( CHO ) cells . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| The mRNA coding for four different spliced variants of the PACAP Type 1 receptor were detected . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Experiments with the antagonists suggest that the response to PACAP is via the PACAP type 1 receptor . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Three cloned G protein coupled 7 transmembrane spanning receptors bind PACAP with high affinity , one reacting only weakly with VIP ( PACAP type 1 receptor ) the other two binding vasoactive intestinal peptide ( VIP ) with equally high affinity ( VIP type 1 and 2 receptors ) . ^^^ The PACAP type 1 receptor displays high affinity for both variants of PACAP . ^^^ In this study , we have investigated the distribution of PACAP and the PACAP type 1 receptor in the adrenal medulla of newborn and adult rat . ^^^ By in situ hybridization we detected PACAP type 1 receptor mRNA in a majority of the adrenal medullary cells of both newborn and adult rat . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Biochemical and cloning experiments have demonstrated that there are two structurally distinct receptors which recognize PACAP and VIP peptides with similar affinities ( PACAP / VIP R 1 , PACAP / VIP R 2 ) , as well as a receptor that is specific for the PACAP peptide ( PACAP Type 1 receptor ) . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Here , we report cloning of a novel PACAP receptor variant , designated PACAPR TM 4 ( transmembrane domain 4 ) , that differs from the previously cloned short form of the PACAP receptor ( PACAPR ) primarily by discrete sequences located in transmembrane domains 2 and 4 . ^^^ Reverse transcriptase polymerase chain reaction and primer extension analyses demonstrated tissue specific differential expression of mRNAs encoding PACAPR TM 4 and splice variant forms of the PACAP receptor . ^^^ However , unlike the PACAPR , PACAPR TM 4 does not activate either adenylyl cyclase or phosphoinositide phospholipase C in response to PACAP in either transient or stable expression systems . ^^^ However , PACAP stimulates increases in [ Ca2+ ] 1 in cells expressing PACAPR TM 4 by activating L type Ca2+ channels , a response not elicited by stimulation with vasoactive intestinal polypeptide . ^^^ The signaling phenotype of PACAPR TM 4 is characteristic of the PACAP receptor involved in regulation of insulin secretion from pancreatic beta islets , a tissue expressing transcripts for PACAPR TM 4 but not for PACAPR or its longer splice variant forms . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| PACAP type 1 receptor gene expression in the paraventricular and supraoptic nuclei of rats . ^^^ PACAP type 1 receptor gene was expressed moderately in the whole area of the PVN and the SON . ^^^ The results are consistent with the hypothesis that neurosecretory cells in the PVN and the SON might be modulated by PACAP through PACAP type 1 receptor . . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| The phase shifting effect is channeled to the clock via a PACAP R 1 receptor , because mRNA from this receptor was demonstrated in the ventral SCN by in situ hybridization . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| The present study suggests that adrenal catecholamine secretion induced by exogenous VIP may be mediated by a PACAP related mechanism , most probably through a PACAP type 1 receptor , in anesthetized dogs . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Thus , the activation signal from the PACAP type 1 receptor on the ERK1 / 2 cascade pathway is received downstream of Ras , either at Raf or MEK . ^^^ We conclude that activation of PACAP type 1 receptor activates protein kinase C , which then activates the ERK1 / 2 cascade in a Ras independent manner at either Raf or MEK1 / 2 . . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| There are at least two types of receptor for PACAP : type 1 ( PACAPR ) , which specifically binds PACAP ; and type 2 ( VIP / PACAPR ) , which binds both PACAP and VIP . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| PACAP receptor ( PACAPR ) mRNA was expressed at high levels in AVP containing neurons in the SON , but at very low levels in oxytocin containing neurons . ^^^ These findings suggest that PACAP serves as a transmitter and / or modulator and the activation of PACAPR stimulates a cAMP protein kinase A pathway which in turn evokes the Ca2+ signaling system . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Pituitary adenylate cyclase activating polypeptide ( PACAP ) has been reported to stimulate melanotroph secretion , and PACAP like immunoreactivity and expression of PACAP type 1 receptor messenger RNA have been identified in the pituitary pars intermedia ( PI ) . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| PACAP type 1 receptor activation promotes cerebellar neuron survival through the cAMP / PKA signaling pathway . ^^^ We show that cerebellar neurons express several PACAP type 1 receptor ( PVR 1 ) isoforms , including the short ( PVR Is ) and the Hop ( PVR 1 Hop ) splice variants , the latter being restricted to neurons and not found in cerebellar glial cell cultures . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Photoaffinity labeling analysis of the interaction of pituitary adenylate cyclase activating polypeptide ( PACAP ) with the PACAP type 1 receptor . ^^^ Despite its lower receptor affinity , [ Bz Phe 22 ] PACAP ( 1 27 ) peptide labeled the PACAP type 1 receptor in pig brain membranes and the rat receptor expressed in COS cells with much higher efficiency ( 20 fold for the pig receptor ) than [ Bz Phe 6 ] PACAP ( 1 27 ) peptide . ^^^ These findings suggest that Tyr 22 in PACAP ( 1 27 ) peptide is located in or close to the hormone binding site of the PACAP type 1 receptor . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| In particular , multiple substituted analogues of PACAP by VIP , and vice versa , demonstrated that positions 4 , 5 and 9 play a dominant role in the recognition of PACAP Type 1 receptor in rat brain membranes and account for the differences observed between PACAP and VIP . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Both pituitary adenylate cyclase activating polypeptide ( PACAP ) and PACAP type 1 receptor gene expressions were detected in the corpus luteum of pregnant mare ' s serum gonadotropin ( PMSG ) human chorionic gonadotropin ( hCG ) treated immature rats using reverse transcription polymerase chain reaction ( RT PCR ) . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| The effects of axotomy , chemical sympathectomy and preganglionic denervation on the expression of the neuropeptides , pituitary adenylate cyclase activating peptide ( PACAP ) , galanin ( GAL ) , and the PACAP type 1 receptor in the rat superior cervical ganglion ( SCG ) were investigated by immunocytochemistry , in situ hybridization and receptor autoradiography . ^^^ In contrast , there was a marked decline in PACAP type 1 receptor expression . ^^^ Preganglionic denervation led to the disappearance of PACAP and VAChT IR baskets and to the upregulation of PACAP and GAL expression in neurons located close to the entrance of the sympathetic chain , whereas PACAP type 1 receptor expression was not affected . ^^^ Taken together , these results indicate that disruption of target contact and / or the infliction of an injury to the axons of the sympathetic neurons , rather than the preganglionic output , regulates the expression of PACAP , GAL and the PACAP type 1 receptor . . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Taken together , the results presented here indicate a modulatory effect of ligands of PACAP type 1 receptor on cytokine production by macrophages and suggest that activation of this receptor , with the subsequent elevation of intracellular cAMP in macrophages , could participate in a negative feedback mechanism that controls certain inflammatory responses . . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| The technique of reverse transcription and polymerase chain reaction with primers specific to PACAP type 1 receptor were used to demonstrate the expression of mRNA encoding PACAP type IA receptor in these cells . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| We have recently demonstrated that maxadilan is a specific agonist for the PACAP type 1 receptor , which is widely distributed in brain . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Together with a goldfish PACAP type 1 receptor and a VIP 1 receptor recently isolated in our laboratory , characterization of this putative GHRH receptor provides the molecular basis for the future understanding of the neuroendocrine control of growth and reproduction by these neuropeptides in goldfish as well as other teleosts . . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Delayed up regulation of Zac 1 and PACAP type 1 receptor after transient focal cerebral ischemia in mice . ^^^ Moreover , we observed an up regulation of PACAP type 1 receptor mRNA expression showing a similar temporospatial distribution . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Pituitary adenylate cyclase activating peptide ( PACAP ) and PACAP type 1 receptor expression in regenerating adult mouse and rat superior cervical ganglia in vitro . ^^^ In contrast , PACAP type 1 receptor mRNA was expressed in virtually all cell bodies within the ganglia . ^^^ After removal and culturing of the SCG for 24 h , there was a marked increase in PACAP mRNA , whilst PACAP type 1 receptor mRNA expression appeared to be downregulated in most nerve cell bodies except for a few scattered neurons displaying a strong upregulation . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Identification of pituitary adenylate cyclase activating polypeptide ( PACAP ) and PACAP type 1 receptor in human skin : expression of PACAP 38 is increased in patients with psoriasis . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Induction of the PAC 1 R ( PACAP type 1 receptor ) gene by p 53 and Zac . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| The capacity of this deletion mutant to interact with COS cells transfected with the PACAP type 1 receptor revealed that this peptide was a specific antagonist to the PACAP type 1 receptor . . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| PACAP type 1 receptor activation regulates ECL cells and gastric acid secretion . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Treatment with the PACAP receptor antagonists PACAP 6 38 ( PACAP type 1 receptor antagonist ) and ( p chloro D Phe ( 6 ) , Leu ( 17 ) ) VIP ( PACAP type 2 receptor antagonist ) blocked the effects of PACAP 38 on the inhibition of transforming growth factor beta 1 ( TGF beta 1 ) induced apoptosis , confirming the specificity of the role of PACAP . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| PACAP induced transcription from the PACAP promoter through PACAP type 1 receptor ( PAC 1 receptor ) . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| The PACAP type 1 receptor agonist maxadilan from sand fly saliva protects mice against lethal endotoxemia by a mechanism partially dependent on IL 10 . ^^^ These results indicate that activation of the PACAP type 1 receptor may contribute to the control of systemic inflammation by a mechanism that is partially dependent on IL 10 . . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Each of the two zebrafish PACAP receptors shares about 70 % sequence identity at the amino acid level with the human PACAP type 1 receptor , and about 50 % amino acid identity with PACAP / VIP R 1 and PACAP / VIP R 2 receptors . ^^^ These results demonstrate that zebrafish PACAP type 1 receptor is a structural and pharmacological homolog of the mammalian PACAP type 1 receptor . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| These findings suggest that Tyr 22 and Lys 15 in PACAP ( 1 27 ) are located in or close to the hormone binding site of the PACAP type 1 receptor . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Maxadilan is a specific agonist and its deleted peptide ( M 65 ) is a specific antagonist for PACAP type 1 receptor . ^^^ In the present study , we demonstrated that maxadilan is a specific agonist of PACAP type 1 receptor ( PACAP / VIP receptor 1 ; PVR 1 ) as determined by the binding assay of [ 125I ] PACAP 27 and cAMP accumulation using CHO cells stably expressing PVR 1 , VIP 1 receptor ( PVR 2 ) , and VIP 2 receptor ( PVR 3 ) , and that the deleted peptide ( # 25 41 ) of maxadilan ( termed as M 65 ) is a specific antagonist of PVR 1 . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| PAC 1 ( pituitary adenylate cyclase activating polypeptide type 1 receptor ) is a G protein coupled receptor that binds the strongly conserved neuropeptide PACAP ( pituitary adenylate cyclase activating polypeptide ) with a thousandfold higher affinity than the related peptide VIP ( vasoactive intestinal peptide ) . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| The PACAP induced catecholamine output responses were inhibited by the PACAP type 1 receptor antagonist PACAP ( 6 38 ) ( 30 3 , 000 nM ) but were resistant to the PACAP type 2 receptor antagonist [ Lys 1 , Pro 2 , 5 , Ara 3 , 4 , Tyr 6 ] vasoactive intestinal peptide ( LPAT VIP ; 30 3 , 000 nM ) . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| These data suggest that , in addition to the PACAP type 1 receptor present on granulosa cells , different subtypes of PACAP receptors are present in the different ovarian compartments . . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| The present studies further evaluated the cell type specific expression and gonadotropin regulation of PACAP type 1 receptor ( PACAPR ) messenger RNA in immature rat ovaries and in cultured preovulatory follicles . ^^^ These results demonstrate the expression of PACAPR messenger RNA in granulosa cells of growing follicles and of preovulatory follicles stimulated by gonadotropins , and suggest that PACAP may play a role in the growth of developing follicles and in ovulation as an autocrine / paracrine factor . . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| We mutated and deleted an amino acid residue ( E 261 ) which is located within the second intracellular loop of the rat PACAP type 1 receptor and which is highly conserved among the receptor family . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| The PACAP type 1 receptor in these cells couples to both adenylyl cyclase and phospolipase C pathways , but although phospolipase C has been implicated in the response to PACAP , the role of adenylyl cyclase is unclear . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| To map in detail the brain areas in which pituitary adenylate cyclase activating polypeptide ( PACAP ) may play a significant role in birds , the distribution of PACAP and PACAP type 1 receptor ( PAC ( 1 ) R ) mRNA was examined throughout the entire chicken brain by using in situ hybridization histochemistry . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Furthermore , PACAP and PACAP type 1 receptor mRNA expression were measured by reverse transcription polymerase chain reaction ( RT PCR ) , and testicular PACAP concentations by RIA . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Recently , the possibility was demonstrated that maxadilan binds to PAC 1 receptor ( PACAP , pituitary adenylate cyclase activating polypeptide type 1 receptor ) in mammals . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Essential structural motif in the C terminus of the PACAP type 1 receptor for signal transduction and internalization . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Here we report the existence of a heretofore unrecognized exon in the extracellular domain of the PACAP type 1 receptor ( PAC1R ) that is alternatively spliced during the spermatogenic cycle in the rat testis . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| PACAP 6 38 , a specific PACAP type 1 receptor antagonist , completely inhibited the amelioration of glutamate induced death and the decrease of intracellular calcium concentration induced by PACAP in cultured hippocampal neurons . ^^^ The data suggest that PACAP has a neuroprotective effect on the hippocampal neuronal damage induced by glutamate , which is related to an inhibition of glutamate induced increase of intracellular calcium concentration and mediated by PACAP type 1 receptor . . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Expression of pituitary adenylate cyclase activating polypeptide and PACAP type 1 receptor in the rat gastric and colonic myenteric neurons . ^^^ In the present study , the pattern of distribution of PACAP and PACAP type 1 receptor ( PAC 1 ) immunoreactivities were examined in the rat stomach and distal colon using a specific polyclonal antibody raised against rat / human PAC 1 . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Pituitary adenylate cyclase activating polypeptide ( PACAP ) is involved in melatonin release via the specific receptor PACAP r 1 , but not in the circadian oscillator , in chick pineal cells . ^^^ We investigated whether PACAP is involved in melatonin release and the circadian oscillation system in chick pineal cells , and if so , whether its effects are mediated by the PACAP specific receptor ( PACAP r 1 ) or the vasoactive intestinal polypeptide ( VIP ) receptor . ^^^ The expression of mRNA for the PACAP r 1 ( including its splicing variant with a hop cassette ) was observed in chick pineal cells . ^^^ These results suggest that PACAP participates in melatonin release , but not in the circadian oscillator system , via the specific receptor PACAP r 1 in chick pineal cells . . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Up regulation of the PACAP type 1 receptor ( PAC 1 ) promoter by neurotrophins in rat PC 12 cells and mouse cerebellar granule cells via the Ras / mitogen activated protein kinase cascade . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Impaired long term potentiation in vivo in the dentate gyrus of pituitary adenylate cyclase activating polypeptide ( PACAP ) or PACAP type 1 receptor mutant mice . ^^^ The present study was conducted to clarify a role of pituitary adenylate cyclase activating polypeptide ( PACAP ) and PACAP type 1 receptor ( PAC1R ) in learning and memory function . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Differential expression of glutamate transporters EAAT 1 and EAAT 2 in mice deficient for PACAP type 1 receptor . ^^^ Since nanomolar concentrations of PACAP exert this effect , signal transduction via the high affinity PACAP type 1 receptor PAC 1 was assumed . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| PACAP type 1 receptor and its mRNA were detected in retinas since E 6 . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| The gene encoding the human type 1 pituitary adenylate cyclase activating polypeptide receptor ( ADCYAP1R1 ) was mapped to chromosome 7 by PCR analysis of genomic DNA from a human / rodent somatic cell hybrid mapping panel . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Molecular structure analysis of the pituitary adenylate cyclase activating polypeptide type 1 receptor from pig brain . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Localization and gene expression of pituitary adenylate cyclase activating polypeptide receptor ( PACAPR ) in the rat retina were studied by immunocytochemistry and in situ hybridization , respectively . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Multiple splice variants of the pituitary adenylate cyclase activating polypeptide type 1 receptor detected by RT PCR in single rat pituitary cells . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| The pituitary adenylate cyclase activating polypeptide type 1 receptor ( PAC 1 R ) is expressed on gastric ECL cells : evidence by immunocytochemistry and RT PCR . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Characterization and messenger ribonucleic acid distribution of a cloned pituitary adenylate cyclase activating polypeptide type 1 receptor in the frog Xenopus laevis brain . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Identification of an essential amino acid motif within the C terminus of the pituitary adenylate cyclase activating polypeptide type 1 receptor that is critical for signal transduction but not for receptor internalization . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Dissociation between light induced phase shift of the circadian rhythm and clock gene expression in mice lacking the pituitary adenylate cyclase activating polypeptide type 1 receptor . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Fahrenholz , A mutation of second intracellular loop of pituitary adenylate cyclase activating polypeptide type 1 receptor confers constitutive receptor activation , FEBS Lett . 469 ( 2000 ) ) . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Morphine withdrawal is modified in pituitary adenylate cyclase activating polypeptide type 1 receptor deficient mice . ^^^ The pituitary adenylate cyclase activating polypeptide type 1 receptor ( PAC 1 ) is a G protein coupled receptor that is widely expressed in neurons of the central and peripheral nervous system . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Assignments of the genes for rat pituitary adenylate cyclase activating polypeptide ( Adcyap 1 ) and its receptor subtypes ( Adcyap1r1 , Adcyap1r2 , and Adcyap1r3 ) . ^^^ Chromosomal assignments of the genes for rat pituitary adenylate cyclase activating polypeptide ( Adcyap 1 ) and all of its receptor subtypes ( Adcyap1r1 , Adcyap1r2 , and Adcyap1r3 ) were performed by PCR analysis of somatic cell hybrid DNAs . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| Expression of pituitary adenylate cyclase activating polypeptide type 1 receptor ( PAC1R ) in the ewe hypothalamus : distribution and colocalization with tyrosine hydroxylase immunoreactive neurones . ^^^ We have examined the distribution of the pituitary adenylate cyclase activating polypeptide type 1 receptor ( PAC1R ) in the ewe hypothalamus by reverse transcription polymerase chain reaction , in situ hybridization and immunohistochemistry . ^^^ |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P18509 and P41586 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.52846846 |
| Pituitary adenylate cyclase activating polypeptide type 1 receptor ( PACAPr ) belongs to the novel subfamily of the G protein coupled receptors with a long extracellular N terminus , which functions as a major binding site for the PACAP . 0.52846846^^^ Pituitary adenylate cyclase activating polypeptide type 1 receptor ( PACAPr ) belongs to the novel subfamily of the G protein coupled receptors with a long extracellular N terminus , which functions as a major binding site for the PACAP . 0.51843732^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| The phase shifting effect is channeled to the clock via a PACAP R 1 receptor , because mRNA from this receptor was demonstrated in the ventral SCN by in situ hybridization . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Pituitary adenylate cyclase activating polypeptide ( PACAP ) is involved in melatonin release via the specific receptor PACAP r 1 , but not in the circadian oscillator , in chick pineal cells . ^^^ We investigated whether PACAP is involved in melatonin release and the circadian oscillation system in chick pineal cells , and if so , whether its effects are mediated by the PACAP specific receptor ( PACAP r 1 ) or the vasoactive intestinal polypeptide ( VIP ) receptor . ^^^ The expression of mRNA for the PACAP r 1 ( including its splicing variant with a hop cassette ) was observed in chick pineal cells . ^^^ These results suggest that PACAP participates in melatonin release , but not in the circadian oscillator system , via the specific receptor PACAP r 1 in chick pineal cells . . ^^^ Pituitary adenylate cyclase activating polypeptide ( PACAP ) is involved in melatonin release via the specific receptor PACAP r 1 , but not in the circadian oscillator , in chick pineal cells . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| The data suggest that the effects of both peptides are mediated through a common PACAP preferring receptor that is pharmacologically different from the mammalian PACAP type 1 receptor . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| We have addressed the question whether this extracellular amino terminus of the PACAP type 1 receptor can solely function as a PACAP binding domain . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Pharmacological properties of two recombinant splice variants of the PACAP type 1 receptor , transfected and stably expressed in CHO cells . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Chinese hamster ovary ( CHO ) K 1 cells transfected with the cDNA specifically bound PACAP and mediated PACAP triggered intracellular accumulation of cAMP , indicating that this cDNA encoded a functional human PACAP Type 1 receptor . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Messenger RNA analysis indicated that at least three splice variants of the PACAP type 1 receptor may be expressed in 6 / 23 cells . . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| After a brief overview of characterized GPCR splice variants , we will describe in more detail the functional properties of the PACAP type 1 receptor splice variants . ^^^ The PACAP type 1 receptor is therefore one of the few examples in which alternative splicing is clearly linked to functional diversity . . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Here we report the molecular cloning and functional expression of the PACAP type 1 receptor isolated from an AR 4 2J cell cDNA library by cross hybridization screening with a rat VIP receptor cDNA . ^^^ Results of binding studies and stimulation of cellular cAMP accumulation in COS 7 cells transfected with this cDNA are characteristic of a PACAP type 1 receptor . ^^^ Cloning of the PACAP type 1 receptor will enhance our understanding of its distribution , structure , and functional properties and ultimately increase our understanding of its physiological role . . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| The order of potency of these ligands was PACAP 38 PACAP 27 > VIP > PHI , suggesting that the receptor corresponds to the pharmacologically characterised PACAP Type 1 receptor . . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| The PACAP type 1 receptor ( found in hypothalamus , brain stem , pituitary , adrenal gland and testes ) , specific for PACAP , is positively coupled to adenylyl cyclase and phospholipase C . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Pituitary adenylate cyclase activating polypeptide ( PACAP ) analogues were tested for their ability to occupy the recombinant selective PACAP receptors ( PACAP type 1 receptor ) or the non selective PACAP vasoactive intestinal polypeptide ( VIP ) receptors ( PACAP type 2 , VIP 1 and VIP 2 receptors ) stably transfected and expressed in Chinese hamster ovary ( CHO ) cells . ^^^ Pituitary adenylate cyclase activating polypeptide ( PACAP ) analogues were tested for their ability to occupy the recombinant selective PACAP receptors ( PACAP type 1 receptor ) or the non selective PACAP vasoactive intestinal polypeptide ( VIP ) receptors ( PACAP type 2 , VIP 1 and VIP 2 receptors ) stably transfected and expressed in Chinese hamster ovary ( CHO ) cells . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| The mRNA coding for four different spliced variants of the PACAP Type 1 receptor were detected . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Experiments with the antagonists suggest that the response to PACAP is via the PACAP type 1 receptor . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Three cloned G protein coupled 7 transmembrane spanning receptors bind PACAP with high affinity , one reacting only weakly with VIP ( PACAP type 1 receptor ) the other two binding vasoactive intestinal peptide ( VIP ) with equally high affinity ( VIP type 1 and 2 receptors ) . ^^^ The PACAP type 1 receptor displays high affinity for both variants of PACAP . ^^^ In this study , we have investigated the distribution of PACAP and the PACAP type 1 receptor in the adrenal medulla of newborn and adult rat . ^^^ By in situ hybridization we detected PACAP type 1 receptor mRNA in a majority of the adrenal medullary cells of both newborn and adult rat . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Biochemical and cloning experiments have demonstrated that there are two structurally distinct receptors which recognize PACAP and VIP peptides with similar affinities ( PACAP / VIP R 1 , PACAP / VIP R 2 ) , as well as a receptor that is specific for the PACAP peptide ( PACAP Type 1 receptor ) . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| The present study suggests that adrenal catecholamine secretion induced by exogenous VIP may be mediated by a PACAP related mechanism , most probably through a PACAP type 1 receptor , in anesthetized dogs . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Thus , the activation signal from the PACAP type 1 receptor on the ERK1 / 2 cascade pathway is received downstream of Ras , either at Raf or MEK . ^^^ We conclude that activation of PACAP type 1 receptor activates protein kinase C , which then activates the ERK1 / 2 cascade in a Ras independent manner at either Raf or MEK1 / 2 . . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Pituitary adenylate cyclase activating polypeptide ( PACAP ) has been reported to stimulate melanotroph secretion , and PACAP like immunoreactivity and expression of PACAP type 1 receptor messenger RNA have been identified in the pituitary pars intermedia ( PI ) . ^^^ Pituitary adenylate cyclase activating polypeptide ( PACAP ) has been reported to stimulate melanotroph secretion , and PACAP like immunoreactivity and expression of PACAP type 1 receptor messenger RNA have been identified in the pituitary pars intermedia ( PI ) . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| In particular , multiple substituted analogues of PACAP by VIP , and vice versa , demonstrated that positions 4 , 5 and 9 play a dominant role in the recognition of PACAP Type 1 receptor in rat brain membranes and account for the differences observed between PACAP and VIP . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| PACAP type 1 receptor gene expression in the paraventricular and supraoptic nuclei of rats . ^^^ PACAP type 1 receptor gene was expressed moderately in the whole area of the PVN and the SON . ^^^ The results are consistent with the hypothesis that neurosecretory cells in the PVN and the SON might be modulated by PACAP through PACAP type 1 receptor . . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| PACAP type 1 receptor activation promotes cerebellar neuron survival through the cAMP / PKA signaling pathway . ^^^ We show that cerebellar neurons express several PACAP type 1 receptor ( PVR 1 ) isoforms , including the short ( PVR Is ) and the Hop ( PVR 1 Hop ) splice variants , the latter being restricted to neurons and not found in cerebellar glial cell cultures . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Photoaffinity labeling analysis of the interaction of pituitary adenylate cyclase activating polypeptide ( PACAP ) with the PACAP type 1 receptor . ^^^ Despite its lower receptor affinity , [ Bz Phe 22 ] PACAP ( 1 27 ) peptide labeled the PACAP type 1 receptor in pig brain membranes and the rat receptor expressed in COS cells with much higher efficiency ( 20 fold for the pig receptor ) than [ Bz Phe 6 ] PACAP ( 1 27 ) peptide . ^^^ These findings suggest that Tyr 22 in PACAP ( 1 27 ) peptide is located in or close to the hormone binding site of the PACAP type 1 receptor . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Both pituitary adenylate cyclase activating polypeptide ( PACAP ) and PACAP type 1 receptor gene expressions were detected in the corpus luteum of pregnant mare ' s serum gonadotropin ( PMSG ) human chorionic gonadotropin ( hCG ) treated immature rats using reverse transcription polymerase chain reaction ( RT PCR ) . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Pituitary adenylate cyclase activating peptide ( PACAP ) and PACAP type 1 receptor expression in regenerating adult mouse and rat superior cervical ganglia in vitro . ^^^ In contrast , PACAP type 1 receptor mRNA was expressed in virtually all cell bodies within the ganglia . ^^^ After removal and culturing of the SCG for 24 h , there was a marked increase in PACAP mRNA , whilst PACAP type 1 receptor mRNA expression appeared to be downregulated in most nerve cell bodies except for a few scattered neurons displaying a strong upregulation . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| The effects of axotomy , chemical sympathectomy and preganglionic denervation on the expression of the neuropeptides , pituitary adenylate cyclase activating peptide ( PACAP ) , galanin ( GAL ) , and the PACAP type 1 receptor in the rat superior cervical ganglion ( SCG ) were investigated by immunocytochemistry , in situ hybridization and receptor autoradiography . ^^^ In contrast , there was a marked decline in PACAP type 1 receptor expression . ^^^ Preganglionic denervation led to the disappearance of PACAP and VAChT IR baskets and to the upregulation of PACAP and GAL expression in neurons located close to the entrance of the sympathetic chain , whereas PACAP type 1 receptor expression was not affected . ^^^ Taken together , these results indicate that disruption of target contact and / or the infliction of an injury to the axons of the sympathetic neurons , rather than the preganglionic output , regulates the expression of PACAP , GAL and the PACAP type 1 receptor . . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Taken together , the results presented here indicate a modulatory effect of ligands of PACAP type 1 receptor on cytokine production by macrophages and suggest that activation of this receptor , with the subsequent elevation of intracellular cAMP in macrophages , could participate in a negative feedback mechanism that controls certain inflammatory responses . . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| The technique of reverse transcription and polymerase chain reaction with primers specific to PACAP type 1 receptor were used to demonstrate the expression of mRNA encoding PACAP type IA receptor in these cells . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| We have recently demonstrated that maxadilan is a specific agonist for the PACAP type 1 receptor , which is widely distributed in brain . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Together with a goldfish PACAP type 1 receptor and a VIP 1 receptor recently isolated in our laboratory , characterization of this putative GHRH receptor provides the molecular basis for the future understanding of the neuroendocrine control of growth and reproduction by these neuropeptides in goldfish as well as other teleosts . . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Delayed up regulation of Zac 1 and PACAP type 1 receptor after transient focal cerebral ischemia in mice . ^^^ Moreover , we observed an up regulation of PACAP type 1 receptor mRNA expression showing a similar temporospatial distribution . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| The PACAP type 1 receptor agonist maxadilan from sand fly saliva protects mice against lethal endotoxemia by a mechanism partially dependent on IL 10 . ^^^ These results indicate that activation of the PACAP type 1 receptor may contribute to the control of systemic inflammation by a mechanism that is partially dependent on IL 10 . . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Each of the two zebrafish PACAP receptors shares about 70 % sequence identity at the amino acid level with the human PACAP type 1 receptor , and about 50 % amino acid identity with PACAP / VIP R 1 and PACAP / VIP R 2 receptors . ^^^ These results demonstrate that zebrafish PACAP type 1 receptor is a structural and pharmacological homolog of the mammalian PACAP type 1 receptor . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| These findings suggest that Tyr 22 and Lys 15 in PACAP ( 1 27 ) are located in or close to the hormone binding site of the PACAP type 1 receptor . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Maxadilan is a specific agonist and its deleted peptide ( M 65 ) is a specific antagonist for PACAP type 1 receptor . ^^^ In the present study , we demonstrated that maxadilan is a specific agonist of PACAP type 1 receptor ( PACAP / VIP receptor 1 ; PVR 1 ) as determined by the binding assay of [ 125I ] PACAP 27 and cAMP accumulation using CHO cells stably expressing PVR 1 , VIP 1 receptor ( PVR 2 ) , and VIP 2 receptor ( PVR 3 ) , and that the deleted peptide ( # 25 41 ) of maxadilan ( termed as M 65 ) is a specific antagonist of PVR 1 . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Identification of pituitary adenylate cyclase activating polypeptide ( PACAP ) and PACAP type 1 receptor in human skin : expression of PACAP 38 is increased in patients with psoriasis . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Induction of the PAC 1 R ( PACAP type 1 receptor ) gene by p 53 and Zac . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| The capacity of this deletion mutant to interact with COS cells transfected with the PACAP type 1 receptor revealed that this peptide was a specific antagonist to the PACAP type 1 receptor . . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| PACAP type 1 receptor activation regulates ECL cells and gastric acid secretion . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Treatment with the PACAP receptor antagonists PACAP 6 38 ( PACAP type 1 receptor antagonist ) and ( p chloro D Phe ( 6 ) , Leu ( 17 ) ) VIP ( PACAP type 2 receptor antagonist ) blocked the effects of PACAP 38 on the inhibition of transforming growth factor beta 1 ( TGF beta 1 ) induced apoptosis , confirming the specificity of the role of PACAP . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| The present studies further evaluated the cell type specific expression and gonadotropin regulation of PACAP type 1 receptor ( PACAPR ) messenger RNA in immature rat ovaries and in cultured preovulatory follicles . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| We mutated and deleted an amino acid residue ( E 261 ) which is located within the second intracellular loop of the rat PACAP type 1 receptor and which is highly conserved among the receptor family . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| The PACAP type 1 receptor in these cells couples to both adenylyl cyclase and phospolipase C pathways , but although phospolipase C has been implicated in the response to PACAP , the role of adenylyl cyclase is unclear . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| To map in detail the brain areas in which pituitary adenylate cyclase activating polypeptide ( PACAP ) may play a significant role in birds , the distribution of PACAP and PACAP type 1 receptor ( PAC ( 1 ) R ) mRNA was examined throughout the entire chicken brain by using in situ hybridization histochemistry . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Furthermore , PACAP and PACAP type 1 receptor mRNA expression were measured by reverse transcription polymerase chain reaction ( RT PCR ) , and testicular PACAP concentations by RIA . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Essential structural motif in the C terminus of the PACAP type 1 receptor for signal transduction and internalization . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Here we report the existence of a heretofore unrecognized exon in the extracellular domain of the PACAP type 1 receptor ( PAC1R ) that is alternatively spliced during the spermatogenic cycle in the rat testis . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| The PACAP induced catecholamine output responses were inhibited by the PACAP type 1 receptor antagonist PACAP ( 6 38 ) ( 30 3 , 000 nM ) but were resistant to the PACAP type 2 receptor antagonist [ Lys 1 , Pro 2 , 5 , Ara 3 , 4 , Tyr 6 ] vasoactive intestinal peptide ( LPAT VIP ; 30 3 , 000 nM ) . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| These data suggest that , in addition to the PACAP type 1 receptor present on granulosa cells , different subtypes of PACAP receptors are present in the different ovarian compartments . . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| PACAP 6 38 , a specific PACAP type 1 receptor antagonist , completely inhibited the amelioration of glutamate induced death and the decrease of intracellular calcium concentration induced by PACAP in cultured hippocampal neurons . ^^^ The data suggest that PACAP has a neuroprotective effect on the hippocampal neuronal damage induced by glutamate , which is related to an inhibition of glutamate induced increase of intracellular calcium concentration and mediated by PACAP type 1 receptor . . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| PACAP induced transcription from the PACAP promoter through PACAP type 1 receptor ( PAC 1 receptor ) . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Expression of pituitary adenylate cyclase activating polypeptide and PACAP type 1 receptor in the rat gastric and colonic myenteric neurons . ^^^ In the present study , the pattern of distribution of PACAP and PACAP type 1 receptor ( PAC 1 ) immunoreactivities were examined in the rat stomach and distal colon using a specific polyclonal antibody raised against rat / human PAC 1 . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Up regulation of the PACAP type 1 receptor ( PAC 1 ) promoter by neurotrophins in rat PC 12 cells and mouse cerebellar granule cells via the Ras / mitogen activated protein kinase cascade . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Impaired long term potentiation in vivo in the dentate gyrus of pituitary adenylate cyclase activating polypeptide ( PACAP ) or PACAP type 1 receptor mutant mice . ^^^ The present study was conducted to clarify a role of pituitary adenylate cyclase activating polypeptide ( PACAP ) and PACAP type 1 receptor ( PAC1R ) in learning and memory function . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Differential expression of glutamate transporters EAAT 1 and EAAT 2 in mice deficient for PACAP type 1 receptor . ^^^ Since nanomolar concentrations of PACAP exert this effect , signal transduction via the high affinity PACAP type 1 receptor PAC 1 was assumed . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| PACAP type 1 receptor and its mRNA were detected in retinas since E 6 . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Molecular structure analysis of the pituitary adenylate cyclase activating polypeptide type 1 receptor from pig brain . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Multiple splice variants of the pituitary adenylate cyclase activating polypeptide type 1 receptor detected by RT PCR in single rat pituitary cells . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| The pituitary adenylate cyclase activating polypeptide type 1 receptor ( PAC 1 R ) is expressed on gastric ECL cells : evidence by immunocytochemistry and RT PCR . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Characterization and messenger ribonucleic acid distribution of a cloned pituitary adenylate cyclase activating polypeptide type 1 receptor in the frog Xenopus laevis brain . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Identification of an essential amino acid motif within the C terminus of the pituitary adenylate cyclase activating polypeptide type 1 receptor that is critical for signal transduction but not for receptor internalization . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Recently , the possibility was demonstrated that maxadilan binds to PAC 1 receptor ( PACAP , pituitary adenylate cyclase activating polypeptide type 1 receptor ) in mammals . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Dissociation between light induced phase shift of the circadian rhythm and clock gene expression in mice lacking the pituitary adenylate cyclase activating polypeptide type 1 receptor . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Fahrenholz , A mutation of second intracellular loop of pituitary adenylate cyclase activating polypeptide type 1 receptor confers constitutive receptor activation , FEBS Lett . 469 ( 2000 ) ) . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| PAC 1 ( pituitary adenylate cyclase activating polypeptide type 1 receptor ) is a G protein coupled receptor that binds the strongly conserved neuropeptide PACAP ( pituitary adenylate cyclase activating polypeptide ) with a thousandfold higher affinity than the related peptide VIP ( vasoactive intestinal peptide ) . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Morphine withdrawal is modified in pituitary adenylate cyclase activating polypeptide type 1 receptor deficient mice . ^^^ The pituitary adenylate cyclase activating polypeptide type 1 receptor ( PAC 1 ) is a G protein coupled receptor that is widely expressed in neurons of the central and peripheral nervous system . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Expression of pituitary adenylate cyclase activating polypeptide type 1 receptor ( PAC1R ) in the ewe hypothalamus : distribution and colocalization with tyrosine hydroxylase immunoreactive neurones . ^^^ We have examined the distribution of the pituitary adenylate cyclase activating polypeptide type 1 receptor ( PAC1R ) in the ewe hypothalamus by reverse transcription polymerase chain reaction , in situ hybridization and immunohistochemistry . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Assignments of the genes for rat pituitary adenylate cyclase activating polypeptide ( Adcyap 1 ) and its receptor subtypes ( Adcyap1r1 , Adcyap1r2 , and Adcyap1r3 ) . ^^^ Chromosomal assignments of the genes for rat pituitary adenylate cyclase activating polypeptide ( Adcyap 1 ) and all of its receptor subtypes ( Adcyap1r1 , Adcyap1r2 , and Adcyap1r3 ) were performed by PCR analysis of somatic cell hybrid DNAs . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| The gene encoding the human type 1 pituitary adenylate cyclase activating polypeptide receptor ( ADCYAP1R1 ) was mapped to chromosome 7 by PCR analysis of genomic DNA from a human / rodent somatic cell hybrid mapping panel . ^^^ |
|
| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Mouse PACAPR 3 is a protein of 437 amino acids that has 50 % and 51 % identity with rat PACAP type 1 and type 2 receptors , respectively . ^^^ Expression of recombinant mouse PACAPR 3 in mammalian cells shows that it binds to vasoactive intestinal polypeptide as well as PACAP 38 and 27 , with a slightly higher affinity for PACAP 38 , and is positively coupled to adenylate cyclase . ^^^ The expression of PACAPR 3 in Xenopus oocytes indicates that calcium activated chloride currents are evoked by PACAP and vasoactive intestinal polypeptide , suggesting that PACAPR 3 can also be coupled to phospholipase C . ^^^ These results suggest that the diverse biological effects of PACAP are mediated by a family of structurally related proteins and that PACAPR 3 participates in the regulation of insulin secretion . . ^^^ |
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| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Localization and gene expression of pituitary adenylate cyclase activating polypeptide receptor ( PACAPR ) in the rat retina were studied by immunocytochemistry and in situ hybridization , respectively . ^^^ |
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| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| Here , we report cloning of a novel PACAP receptor variant , designated PACAPR TM 4 ( transmembrane domain 4 ) , that differs from the previously cloned short form of the PACAP receptor ( PACAPR ) primarily by discrete sequences located in transmembrane domains 2 and 4 . ^^^ Reverse transcriptase polymerase chain reaction and primer extension analyses demonstrated tissue specific differential expression of mRNAs encoding PACAPR TM 4 and splice variant forms of the PACAP receptor . ^^^ However , unlike the PACAPR , PACAPR TM 4 does not activate either adenylyl cyclase or phosphoinositide phospholipase C in response to PACAP in either transient or stable expression systems . ^^^ However , PACAP stimulates increases in [ Ca2+ ] 1 in cells expressing PACAPR TM 4 by activating L type Ca2+ channels , a response not elicited by stimulation with vasoactive intestinal polypeptide . ^^^ The signaling phenotype of PACAPR TM 4 is characteristic of the PACAP receptor involved in regulation of insulin secretion from pancreatic beta islets , a tissue expressing transcripts for PACAPR TM 4 but not for PACAPR or its longer splice variant forms . ^^^ |
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| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| There are at least two types of receptor for PACAP : type 1 ( PACAPR ) , which specifically binds PACAP ; and type 2 ( VIP / PACAPR ) , which binds both PACAP and VIP . ^^^ |
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| Interacting proteins: P41586 and P18509 |
Pubmed |
SVM Score :0.0 |
| PACAP receptor ( PACAPR ) mRNA was expressed at high levels in AVP containing neurons in the SON , but at very low levels in oxytocin containing neurons . ^^^ These findings suggest that PACAP serves as a transmitter and / or modulator and the activation of PACAPR stimulates a cAMP protein kinase A pathway which in turn evokes the Ca2+ signaling system . ^^^ |
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