| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.80481669 |
| Csk formed a complex with the focal adhesion protein paxillin in cells , and its SH 2 domain was shown to interact with pp125FAK and paxillin in vitro . 0.80481669^^^ |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.6900855 |
| Although we have shown that the Csk Src homology 2 domain can bind to several tyrosine phosphorylated proteins , including pp125FAK and paxillin , a majority of protein which bound to Csk was IRS 1 when cells were stimulated by insulin . 0.6900855^^^ |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| This increased motility was accompanied by an EGF induced increase in c Src kinase activity , relocalisation of c Src to the cell periphery and phosphorylation of FAK in the carcinoma cells but not in the adenoma cells . ^^^ |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Specifically , the autophosphorylation site of FAK , which is a reported site of c Src kinase binding , is required for bacterial internalization , as overexpression of a derivative lacking the autophosphorylation site had a dominant interfering effect as well . ^^^ |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| To explore the cellular mechanism whereby TNF alpha regulates phosphorylation of FAK in the liver , we measured c Src kinase activity and the abundance of 3 major protein tyrosine phosphatases ( PTPs ) ( PTP 1B , leukocyte antigen related tyrosine phosphatase [ LAR ] , and src homology 2 domain containing protein tyrosine phosphatase [ SHPTP 2 ] ) in liver homogenates from obese Zucker rats after TNF alpha blockade . ^^^ |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| To elucidate the molecular mechanisms by which human epidermal growth factor receptor / heregulin ( HER2 / HRG ) influence the migratory potential of breast cancer cells , we have used phospho specific antibodies against c Src kinase and focal adhesion kinase ( FAK ) . ^^^ This study establishes that HER2 / HRG signaling selectively upregulates Tyr phosphorylation of c Src at Tyr 215 located within the SH 2 domain , increases c Src kinase activity and selectively upregulates Tyr phosphorylation of FAK at Tyr 861 . ^^^ |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| However , in vitro , Fak and Jak 2 kinases were not directly inhibited by PP 1 , demonstrating the effect of PP 1 on c Src kinase as an upstream activator of Fak . ^^^ |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| In addition to the sensitivity of ERKs to cytoskeletal integrity , GnRH induced FAK and c Src kinase activation were sensitive to these agents and the fibronectin integrin antagonistic RGDS peptide . ^^^ |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Disruption of Ca2+ dependent cell matrix adhesion enhances c Src kinase activity , but causes dissociation of the c Src / FAK complex and dephosphorylation of tyrosine 577 of FAK in carcinoma cells . ^^^ |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| In normal fibroblasts , tyrosine phosphorylated paxillin and focal adhesion kinase pp125FAK , which colocalize at focal adhesion plaques , were the major proteins to which the Csk SH 2 domain bound . ^^^ |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Immunofluorescence microscopy revealed that multiple signaling molecules ( e . g . , pp60c src , pp125FAK , phosphatidylinositol 3 kinase , phospholipase C gamma , and Na+ / H+ antiporter ) involved in both integrin and growth factor receptor signaling pathways became associated with the CSK framework of the FAC within 15 min after binding to beads coated with integrin ligands . ^^^ Western blot analysis confirmed that FACs isolated using RGD beads were enriched for pp60c src , pp125FAK , phospholipase C gamma , and the Na+ / H+ antiporter when compared with intact CSK or basal cell surface preparations that retained lipid bilayer . ^^^ |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| These can be divided into nine families : Abl , Fes / Fer , Syk / Zap70 , Jak , Tec , Fak , Ack , Src , and Csk . ^^^ |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| LPA induced tyrosine phosphorylation of SHPS 1 was markedly reduced in either focal adhesion kinase ( FAK ) deficient mouse cells or CHO cells overexpressing the tyrosine kinase CSK . ^^^ |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Significantly , repression of endogenous Src family PTK activity by p 50 ( csk ) overexpression inhibited FN stimulated cell spreading , Pyk 2 tyrosine phosphorylation , Grb 2 binding to Shc , and ERK 2 activation in the FAK but not in FAK+ cells . ^^^ |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| In addition , paxillin , a Src and / or pp 125 ( FAK ) substrate , displayed increased levels of tyrosine phosphorylation in PTPalpha expressing cells and was associated with elevated amounts of Csk . ^^^ |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| On the other hand , overexpression of Csk DeltaK induced tyrosine phosphorylation of cellular proteins , including the paxillin and focal adhesion kinase ( FAK ) and enhanced to some extent the cytoskeletal organization and the rate of cell spreading on fibronectin , indicating that Src or its relatives was functionally activated in the cells . ^^^ In contrast , tyrosine phosphorylation of FAK and its in vitro autophosphorylation activity were increased only in cells expressing Csk DeltaK . ^^^ In Csk expressing cells , the kinase activity of FAK was substantially decreased to 20 30 % that of control cells , even though the expression level of FAK was rather increased . ^^^ These findings suggest that Csk regulates Src family tyrosine kinases that play essential roles in the regulation of cell adhesion via a FAK dependent mechanism and that the tyrosine phosphorylation of paxillin alone may not be sufficient for the regulation of the cell adhesion mechanism in astrocytes . . ^^^ |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| FAK associates with CSK 48 h after infection and recruits it to focal contacts . ^^^ De novo expression of pp125FAK in human macrophages regulates CSK distribution and MAP kinase activation but does not affect focal contact structure . ^^^ |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Expression of the FAK Phe 397 mutant did not promote FAK cell migration and overexpression of p 50 ( csk ) in DA 2 cells inhibited migration to FN suggesting that Src family PTKs play important roles in FAK mediated motility events . ^^^ |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| We demonstrate that erythroid progenitor cells express several receptor and non receptor tyrosine kinases , like c kit , Jak 1 , Ryk , FAK , Syk , Arg , Csk and members of the insulin receptor family . ^^^ |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Abbreviations : CAS , CRK associated substrate ; CH , calponin homology domain ; CSK , C terminal SRC kinase ; E 6 , Papillomavirus E 6 protein ; FAK , focal adhesion kinase ; GIT , GRK interacter ; GPCR , heterotrimeric G protein coupled receptor ; GRK , G protein coupled receptor kinase ; MAPK , mitogen activated protein kinase ( ERK , p 38 , JNK ) ; PAK , p 21 activated kinase ; PBS , paxillin binding subdomain ; PIX , PAK interacting exchange factor ; PKL , paxillin kinase linker ; POR 1 , partner of Rac ; PS , phosphoserine ; PT , phosphothreonine ; PY , phosphotyrosine ; RTK , growth factor receptor tyrosine kinase ; SH , SRC homology domain . . ^^^ |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Conversely , expression of a dominant negative Csk resulted in elevated SFK activation , enhanced phosphorylation of FAK and paxilllin , enhanced cell scattering , an increased number of focal contacts , dramatic rearrangement of actin cytoskeleton and increased cell adhesion / migration and in vitro invasiveness . ^^^ |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Tyrosine phosphorylated CagA binds and activates SHP 2 phosphatase and the C terminal Src kinase ( Csk ) while inducing an elongated cell shape termed the `` hummingbird phenotype . ' ' Here we show that CagA reduces the level of focal adhesion kinase ( FAK ) tyrosine phosphorylation in gastric epithelial cells . ^^^ The decrease in phosphorylated FAK is due to SHP 2 mediated dephosphorylation of FAK at the activating phosphorylation sites , not due to Csk dependent inhibition of SFKs , which phosphorylate FAK . ^^^ |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Activated Src ( SrcY529F ) as well as activation of putative Src signaling mediators ( Fak , Cas , Crk / CrkL , C3G , and Rap 1 ) blocked the effect of FA Csk in a manner dependent on Rap 1 . ^^^ |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| Stretch induced activation of p 38 MAPK was abolished by overexpression of FAT and CSK , which are inhibitors of the FAK and Src families , respectively , and was suppressed by overexpression of a dominant negative mutant of Ras . ^^^ |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and Q05397 |
Pubmed |
SVM Score :0.0 |
| NA |
|