| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| VEGF stimulation specifically activated c Src kinase activity but not that of other related Src kinases such as Lyn , Fyn , or Hck in KS cells . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| Rapid and efficient purification of Src homology 2 domain containing proteins : Fyn , Csk and phosphatidylinositol 3 kinase p 85 . ^^^ To analyse the regulation of Src family tyrosine kinases in vitro , we have purified Fyn and Csk , a kinase capable of regulating Fyn activity by phosphorylation , from baculovirus infected insect cells . ^^^ The binding of Csk to different phosphopeptides was tested , and specificity for the autophosphorylation site of Fyn was demonstrated . ^^^ Pure Csk was used to phosphorylate Fyn and down regulate its kinase activity , and the kinetic parameters of both the active and the repressed forms of Fyn were determined . ^^^ Repression of Fyn activity by Csk reduced binding of Fyn to phosphopeptides to undetectable levels , supporting the model that predicts an intramolecular interaction of the Fyn SH 2 domain with a C terminal phosphotyrosine residue . . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| Consistent with the decrease in the Csk level , the kinase activity of a member of the Src family , Fyn , was greatly enhanced , but that of Src was not correlated inversely with the level of Csk expression . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| To determine the role of activated Src family kinases in the csk phenotype , we have introduced mutations in the src and fyn genes into the csk mutant background . ^^^ Genetic analysis reveals that src , but not fyn , is partly epistatic to the csk gene . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| The simultaneous overproduction in E . coli of the chaperones DnaK , DnaJ and GrpE on the one hand , or GroEL and GroES on the other hand , and the human non receptor protein tyrosine kinases Csk , Fyn or Lck resulted in increased solubility of the recombinant kinases . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| There was about a 30 fold decrease in frequency of G 418 resistant colonies with this strategy against that using only the neo gene in the vector , and homologous recombinants were obtained at frequencies of more than 1 / 50 among G 418 resistant cells at fyn , csk , c mos , and insulin receptor substrate 1 gene loci . . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| Functional and physical interaction of protein tyrosine kinases Fyn and Csk in the T cell signaling system . ^^^ Csk , which phosphorylates tyrosine residues in the negative regulatory sites of Src family kinases , down regulated Fyn and Lck mediated stimulation of the serum response element and Fyn mediated enhancement of IL 2 promoter activity . ^^^ These data suggest that Fyn and Lck , whose activities are regulated by Csk , are involved in different phases of T cell activation . . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| Csk may be involved in the down regulation of T cell receptor ( TCR ) signaling by C terminal tyrosine phosphorylation of Lck and Fyn ; however , it is not known how Csk activity is regulated or how it targets these Src family members . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| The precipitation of Nef by the Lck recombinant proteins was specific , since neither Fyn , Csk , p 85 phosphatidylinositol 3 kinase nor phospholipase Cgamma SH 2 domains coprecipitated Nef from T cells . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| We determined whether differences in phosphorylation between naive and proliferating T cells were due to altered regulation of p56lck ( Lck ) or p59fyn ( Fyn ) by their positive or negative regulators , CD 45 or p5Ocsk ( Csk ) , respectively . ^^^ Therefore , although the protein expression level of CD 45 was increased in proliferating T cells it only partially compensated for the hyperactivity of Csk resulting in a 2 fold reduction in the specific activity of Lck and Fyn in proliferating T cells . . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| Impaired development of CD4+ CD8+ thymoyctes by csk ' knock in ' into fyn locus . p59fyn is one of the Src family kinases thought to play an important role in signaling through T cell receptor . ^^^ Here , we have ' knocked in ' the csk gene , a negative regulator of Src family kinases , into fyn locus to challenge the problem of redundant functions among Src family kinases . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| An association between active Cdc 2 and the Src related kinases Lyn and Fyn was also demonstrated , although Cdc 2 was not found associated with the tyrosine kinases , Csk and Syk . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| In co transfected COS cells , Lck and Fyn caused phosphorylation of LMPTP , whereas Csk , Zap , and Jak 2 did not . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| Binding of FYN and SHC to the diphosphorylated Tyr568 / 570 site was observed , while binding of Csk to this site was not observed . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| Expression of Src / Csk , but not DK Src / Csk , caused reduction of the specific activities of Src and Fyn in the differentiated P 19 cells . ^^^ In the Src / Csk transfectant , L 1 stimulation induced delayed activation of Src and Fyn peaking at much lower levels than in the control cells . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| Csk is probably involved in the downregulation of TCR signaling by C terminal tyrosine phosphorylation of Lck and Fyn , but the mechanism whereby Csk targets these Src family members is not known . ^^^ The interaction with TCR brings Csk into close contact with Lck and Fyn which are known to be associated with TCR , either functionally and / or physically . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| Initial results implicate a role for the CSK protein tyrosine kinase as responsible for the abnormal Fyn kinase activity observed in HIV infected patients . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| These responses were markedly reduced in cells overexpressing the Csk kinase or in cells that lack focal adhesion kinase or the Src family kinases Src or Fyn . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| Our results reveal that the non receptor tyrosine kinases , Abl , Fyn , Lyn , Btk and Csk , are expressed in all three cell types . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| Recombinant CD 45 AP exhibited specific binding to Lck and ZAP 70 protein tyrosine kinases , but not to Fyn or Csk , in lysates of both CD 45 positive and negative T cells . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| RPDE 6 exhibited selectivity in binding to the SH 3 domains of c Abl , Crk , Csk , Lck , Lyn , Fyn and 5 Src , with binding to the SH 3 regions of the Src related tyrosyl kinases Lyn and Fyn being the most effective . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| Furthermore , similar to anergic T cells , Fyn is hyperactive in one of these HTLV 1 infected T cell lines , probably as a consequence of Csk downregulation . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| We also observed that acute or chronic ethanol treatments did not alter the Fyn or Csk levels in cortical neurons . ^^^ Although Fyn , but not Csk , was present in adult cerebral cortex , ethanol did not phosphorylate the R2B subunit in this region . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| Here , we report that loss of csk resulted in a reduction in the abundance of the Src and Fyn proteins , which could be restored by reintroducing catalytically active Csk . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| The proline , glutamic acid , serine and threonine enriched protein tyrosine phosphatase PEP , which is expressed primarily in hematopoietic cells , was recently discovered to be physically associated with the 50 kDa cytosolic protein tyrosine kinase ( PTK ) Csk , an important suppressor of Src family PTK , including Lck and Fyn in T cells . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| The SH 2 domains of fyn , csk , phosphatidylinositol 3 ' kinase , rasGAP , vav and lck bind to pp 85 90 with varying affinities . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| We show that whereas no autoantibodies were found against cSrc or its relative Fyn , up to 20 % of patients with carcinoma had high affinity autoantibodies against Csk . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| The function of Csk as a repressor of Lck and Fyn activity suggests activation of these PTKs is solely responsible for the phenotype observed in csk deficient T lineage cells . ^^^ We provide genetic evidence for this notion as alpha / beta T cell development is blocked in lck ( / ) fyn ( / ) csk deficient mice . ^^^ It remains unclear whether activation of Lck and Fyn in the absence of Csk uncouples alpha / beta T cell development entirely from engagement of surface expressed receptors . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| Using a GST fusion protein carrying the cytoplasmic tail of beta dystroglycan , we identified five SH 2 domain containing proteins that interact with beta dystroglycan in a phosphorylation dependent manner , including c Src , Fyn , Csk , NCK , and SHC . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| Cutting edge : Fyn is essential for tyrosine phosphorylation of Csk binding protein / phosphoprotein associated with glycolipid enriched microdomains in lipid rafts in resting T cells . ^^^ By analyzing T cells from Fyn / mice , we clearly demonstrated that Fyn , but not Lck , has its kinase activity in membrane rafts , and plays a critical role in Cbp phosphorylation , Cbp Csk interaction , and Csk kinase activity . ^^^ Thus , Fyn mediates Cbp Csk interaction and recruits Csk to rafts by phosphorylating Cbp . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| Interestingly , there was a concomitant increase in the phosphorylation of Fyn on its Csk ( C terminal Src kinase ) site , indicating feedback inhibition . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| The results obtained by using mutant mice or cells that lack Csk and / or a member of SFK , Fyn , reveal that Cbp is phosphorylated predominantly by raft localized Fyn in vivo . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| This became apparent from the hyperresponsive degranulation of lyn / bone marrow derived mast cells , which is driven by hyperactivation of Fyn kinase that occurs , in part , through the loss of negative regulation by COOH terminal Src kinase ( Csk ) and the adaptor , Csk binding protein . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| The two SFKs respond to integrin ligation by different mechanisms : Lyn , by increased autophosphorylation of a catalytic tyrosine ; and Fyn , by reduced Csk phosphorylation of the inhibitory COOH terminal tyrosine . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| Finally , we demonstrate that Fyn and Csk can both phosphorylate 2B4 , suggesting a possible mechanism of 2B4 phosphorylation . . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| The Csk tyrosine kinase negatively regulates the Src family kinases Lck and Fyn in T cells . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| CSK overexpression caused a profound inhibition of NGF induced activation of FYN , YES , RAS , and ERK and inhibited neurite outgrowth , NGF stimulated integrin directed migration and blocked the NGF induced conversion of GDP RAC to its GTP bound active state . ^^^ In contrast , kinase dead CSK augmented the activation of FYN , RAS , and ERK and increased neurite outgrowth . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| Raft proteins increased after activation include Csk , Csk binding protein and Fyn , the molecules known to be involved in negative regulation of T cell activation . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| Lyp is expressed in lymphocytes , where it physically associates through its proline rich motif ( called P 1 ) with the SH 3 domain of the protein tyrosine kinase Csk , an important suppressor of the Src family of kinases Lck and Fyn , which mediate TCR signaling . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| Synthetic peptides containing tyrosine analogues displaying different side chain orientations were analyzed by NMR techniques and tested as potential substrates of the nonreceptor tyrosine kinases Syk , Csk , Lyn , and Fyn . ^^^ |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41240 and P06241 |
Pubmed |
SVM Score :0.0 |
| NA |
|