Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.67657248
First , using real time biospecific interaction analysis ( BIAcore ( TM ) ) , we detected in vitro a specific interaction between Csk and one of its substrates Lck , a lymphocyte specific member of the Src family . 0.67657248^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
In addition , a homology model of c Src kinase with the activation loop resembling the active conformation was constructed using the crystal structure of the kinase domain of Lck . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
Molecular cloning of lsk , a carboxyl terminal src kinase ( csk ) related gene , expressed in leukocytes . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
The results show that CSK has strict sequence specificity for the normal Src sequence , although it can also phosphorylate the Lck sequence . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
IL 4 and IL 13 induce Lsk , a Csk like tyrosine kinase , in human monocytes . ^^^ Lsk is a protein tyrosine kinase with homology to the COOH terminal Src kinase ( Csk ) . ^^^ Unlike Csk that is ubiquitously expressed , Lsk has limited tissue distribution . ^^^ Here we have examined the expression and regulation of Lsk and Csk in peripheral human monocytes . ^^^ These results suggest that despite their structural similarities , Lsk and Csk may play distinct regulatory roles in monocyte functions elicited by cytokines , with Lsk functioning specifically within the context of a Th 2 type immune response . . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
The simultaneous overproduction in E . coli of the chaperones DnaK , DnaJ and GrpE on the one hand , or GroEL and GroES on the other hand , and the human non receptor protein tyrosine kinases Csk , Fyn or Lck resulted in increased solubility of the recombinant kinases . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
Csk , which phosphorylates tyrosine residues in the negative regulatory sites of Src family kinases , down regulated Fyn and Lck mediated stimulation of the serum response element and Fyn mediated enhancement of IL 2 promoter activity . ^^^ These data suggest that Fyn and Lck , whose activities are regulated by Csk , are involved in different phases of T cell activation . . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
Further studies in which Lck and Csk were coexpressed in S . cerevisiae indicated that the absence of the SH 2 domain did not affect the ability of Csk to phosphorylate p56lck at tyrosine 505 . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
Csk may be involved in the down regulation of T cell receptor ( TCR ) signaling by C terminal tyrosine phosphorylation of Lck and Fyn ; however , it is not known how Csk activity is regulated or how it targets these Src family members . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
The precipitation of Nef by the Lck recombinant proteins was specific , since neither Fyn , Csk , p 85 phosphatidylinositol 3 kinase nor phospholipase Cgamma SH 2 domains coprecipitated Nef from T cells . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
We determined whether differences in phosphorylation between naive and proliferating T cells were due to altered regulation of p56lck ( Lck ) or p59fyn ( Fyn ) by their positive or negative regulators , CD 45 or p5Ocsk ( Csk ) , respectively . ^^^ Therefore , although the protein expression level of CD 45 was increased in proliferating T cells it only partially compensated for the hyperactivity of Csk resulting in a 2 fold reduction in the specific activity of Lck and Fyn in proliferating T cells . . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
RPDE 6 exhibited selectivity in binding to the SH 3 domains of c Abl , Crk , Csk , Lck , Lyn , Fyn and 5 Src , with binding to the SH 3 regions of the Src related tyrosyl kinases Lyn and Fyn being the most effective . ^^^ The binding of RPDE 6 to the SH 3 domains of Crk , Csk and Lck led to a marked reduction in PDE activity , but no change was apparent in complexes with other species . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
In co transfected COS cells , Lck and Fyn caused phosphorylation of LMPTP , whereas Csk , Zap , and Jak 2 did not . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
Csk is probably involved in the downregulation of TCR signaling by C terminal tyrosine phosphorylation of Lck and Fyn , but the mechanism whereby Csk targets these Src family members is not known . ^^^ The interaction with TCR brings Csk into close contact with Lck and Fyn which are known to be associated with TCR , either functionally and / or physically . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
The Csk like proteins Lsk , Hyl , and Matk represent the same Csk homologous kinase ( Chk ) and are regulated by stem cell factor in the megakaryoblastic cell line MO7e . ^^^ Recently , the cDNAs for Lsk , Matk and Hyl , three Csk related protein tyrosine kinases , have been cloned . ^^^ These data demonstrate differential regulation of Csk family members by cytokines and suggest a role for Lsk / Hyl / Matk in responses mediated by SCF and PMA . ^^^ Lastly , since our studies strongly suggest that the Lsk , Hyl and Matk kinases are related and regulated distinctly from Csk , we and several of the original authors have agreed to rename this kinase the Csk homologous kinase ( Chk ) . . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
In addition , a catalytically inactive version of the Src family member , Lck ( lymphoid cell kinase ) , was expressed , purified , and evaluated as a Csk substrate . ^^^ Kinetic studies revealed that the chemical step is at least partially rate determining in Csk mediated phosphoryl transfer to the Lck protein . ^^^ The lack of a significant impact of increased salt on the Km for Lck phosphorylation differs from Csk mediated poly ( Glu , Tyr ) phosphorylation , and argues against the importance of electrostatic effects in the Csk Lck binding interaction . ^^^ The failure of the Lck phosphorylation product ( phosphotyrosine 505 ) to significantly inhibit Csk phosphorylation of Lck is consistent with a catalytic model involving multidomain structural interactions between substrate and enzyme . . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
However , an increased number of Csk substrates was detected in NIH3T3 cells expressing a constitutively activated form of the Src kinase Lck or in PC 12 and NIH3T3 cells that had been treated with pervanadate . ^^^ To verify this hypothesis we analyzed Csk mediated phosphorylation of recombinant Lck , a known Csk substrate . ^^^ Results demonstrated that autophosphorylation of Lck ( at Tyr 394 ) facilitates Csk mediated phosphorylation of Lck at its regulatory site ( Tyr 505 ) . ^^^ Subsequent peptide binding studies revealed that Csk can bind to a peptide corresponding to the Lck autophosphorylation site only when it is phosphorylated . ^^^ These findings suggest that autophosphorylation of Lck at Tyr 394 triggers an interaction with Csk and thereby facilitates subsequent phosphorylation and inactivation of Lck . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
Phosphorylation of a physiological substrate ( Lck ) and autophosphorylation of Csk was not inhibited by phosphate , indicating that the phosphate dependent inhibition of Csk activity was substrate specific . . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
Phosphorylation of residue Y 505 in Lck by COOH terminal Src kinase ( Csk ) , which negatively regulates Lck , is essential for the inhibitory effect of cAMP on zeta chain phosphorylation . ^^^ Both PKA type 1 and Csk are targeted to lipid rafts where proximal T cell activation occurs , and phosphorylation of raft associated Lck by Csk is increased in cells treated with forskolin . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
The SH 2 domains of fyn , csk , phosphatidylinositol 3 ' kinase , rasGAP , vav and lck bind to pp 85 90 with varying affinities . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
The function of Csk as a repressor of Lck and Fyn activity suggests activation of these PTKs is solely responsible for the phenotype observed in csk deficient T lineage cells . ^^^ We provide genetic evidence for this notion as alpha / beta T cell development is blocked in lck ( / ) fyn ( / ) csk deficient mice . ^^^ It remains unclear whether activation of Lck and Fyn in the absence of Csk uncouples alpha / beta T cell development entirely from engagement of surface expressed receptors . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
It was shown that the apparent k ( cat ) and K ( m ) values for Csk phosphorylation of catalytically impaired Src ( dSrc ) are similar to the parameters for Csk catalyzed phosphorylation of the Src family member Lck . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
Accordingly , overexpression of p 50 Csk a kinase that inactivates p56Lck induced constitutive activation of ERKs . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
Recombinant CD 45 AP exhibited specific binding to Lck and ZAP 70 protein tyrosine kinases , but not to Fyn or Csk , in lysates of both CD 45 positive and negative T cells . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
Engagement of the T cell receptor ( TCR ) by peptide antigen bound to the major histocompatibility complex molecules initiates a biochemical cascade involving protein tyrosine kinases ( PTKs ) such as Lck , ZAP 70 and Csk , and protein tyrosine phosphatases ( PTPases ) such as CD 45 , SHP 1 and SHP 2 . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
We screened 1680 spatially separated compounds of a diverse combinatorial library of 1 , 4 benzodiazepines for their ability to inhibit the kinase activity of protein tyrosine kinases Src , Yes , Abl , Lck , Csk , and fibroblast growth factor receptor . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
The proline , glutamic acid , serine and threonine enriched protein tyrosine phosphatase PEP , which is expressed primarily in hematopoietic cells , was recently discovered to be physically associated with the 50 kDa cytosolic protein tyrosine kinase ( PTK ) Csk , an important suppressor of Src family PTK , including Lck and Fyn in T cells . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
Furthermore , cotransfection of wild type Lck with C terminal Src kinase ( Csk ) into JCaM1 . 6 failed to restore the chemotactic response induced by CXCL 12 . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
By analyzing T cells from Fyn / mice , we clearly demonstrated that Fyn , but not Lck , has its kinase activity in membrane rafts , and plays a critical role in Cbp phosphorylation , Cbp Csk interaction , and Csk kinase activity . ^^^ Csk recruited to rafts would then be activated and inhibit the kinase activity of Lck to keep resting T cells in a quiescent state . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
Phospho LIME associates with the Src family kinase Lck and its negative regulator , Csk . ^^^ Ectopic expression of LIME in Jurkat T cells results in an increase of Csk in lipid rafts , increased phosphorylation of Lck and higher Ca2+ response to CD 3 stimulation . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
CD 45 dephosphorylated Lck and Src at similar rates but dephosphorylated Csk and Erk 1 at lower rates . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
This study investigated the expression of molecules that regulate LCK homeostasis , such as CD 45 , C terminal Src kinase ( CSK ) , and c Cbl , in lipid raft domains from SLE T cells and investigated the localization of these proteins during T cell receptor ( TCR ) triggering . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
The Csk tyrosine kinase negatively regulates the Src family kinases Lck and Fyn in T cells . ^^^ G3BP reduced the ability of Csk to phosphorylate Lck at Y 505 by decreasing the amount of Csk in lipid rafts . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
Lyp is expressed in lymphocytes , where it physically associates through its proline rich motif ( called P 1 ) with the SH 3 domain of the protein tyrosine kinase Csk , an important suppressor of the Src family of kinases Lck and Fyn , which mediate TCR signaling . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
In contrast , the expression of LAT , which like Lck distributes to raft and non raft membrane , or Csk , a kinase with a structure similar to Lck , is not affected by cell aggregation . ^^^
Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
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Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
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Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
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Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
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Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
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Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
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Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
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Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
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Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
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Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
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Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
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Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
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Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
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Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
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Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
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Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
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Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
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Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
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Interacting proteins: P41240 and P06239	Pubmed	SVM Score :0.0
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