| Interacting proteins: P05161 and P41226 |
Pubmed |
SVM Score :0.0 |
| We identify UBE1L as the E 1 enzyme that catalyzes the first activation step in the conjugation of ISG 15 , and show that the NS1B protein inhibits this activation step in vitro . ^^^ |
|
| Interacting proteins: P05161 and P41226 |
Pubmed |
SVM Score :0.0 |
| The increase in ISG 15 conjugates did not occur through a stabilization of the ISG 15 E1 enzyme , UBE1L . ^^^ |
|
| Interacting proteins: P05161 and P41226 |
Pubmed |
SVM Score :0.0 |
| Involvement of UBE1L in ISG 15 conjugation during retinoid induced differentiation of acute promyelocytic leukemia . ^^^ RA treatment of APL and other RA responsive leukemic cells induced expression of UBE1L and ISG 15 as well as intracellular ISG 15 conjugates . ^^^ Induction of UBE1L and ISG 15 along with ISG 15 conjugation in RA sensitive NB 4 S1 APL cells were detected following treatment with specific retinoids and type 1 interferon ( IFN ) . ^^^ UBE1L and ISG 15 mRNAs were co expressed in normal human tissues that were examined . ^^^ A physical association was found between UBE1L and ISG 15 in vivo . ^^^ |
|
| Interacting proteins: P05161 and P41226 |
Pubmed |
SVM Score :0.0 |
| Isolation and sequence of an interferon tau inducible , pregnancy and bovine interferon stimulated gene product 15 ( ISG 15 ) specific , bovine ubiquitin activating E 1 like ( UBE1L ) enzyme . ^^^ Ubiquitin activating enzyme 1 like protein ( UBE1L ) initiates enzymatic conjugation by forming a thioester bond with ISG 15 , thus preparing it for transfer to the next series of enzymes . ^^^ Because bovUBE1L was purified through its interaction with rGST ISG 15 and shares significant amino acid and cDNA sequence identity with human UBE1L , it is concluded that it mediates conjugation of ISG 15 to uterine proteins in response to the developing and attaching conceptus . . ^^^ |
|
| Interacting proteins: P05161 and P41226 |
Pubmed |
SVM Score :0.0 |
| Like other components of the protein ISGylation system ( ISG 15 , UBE1L , and UBP 43 ) , Ubc 8 is an interferon inducible protein . ^^^ Furthermore , we established an assay system to detect ISGylated target proteins by cotransfection of ISG 15 , UBE1L , and Ubc 8 together with a target protein to be analyzed . ^^^ |
|
| Interacting proteins: P05161 and P41226 |
Pubmed |
SVM Score :0.0 |
| The cap is subsequently removed with carboxypeptidase B to yield mature biologically active ISG 15 capable of stoichiometric ATP dependent thiolester formation with its human UbE1L activating enzyme . ^^^ The C terminal domain of ISG 15 was superimposed on the structure of Nedd 8 ( r . m . s . d . = 0 . 84 A ) bound to its AppBp 1 Uba3 activating enzyme to model ISG 15 binding to UbE1L . ^^^ |
|
| Interacting proteins: P05161 and P41226 |
Pubmed |
SVM Score :0.0 |
| Among the proteins induced by IFN alpha / beta are the ubiquitin like ISG 15 protein and its E 1 ( Ube1L ) and E 2 ( UbcH 8 ) conjugating enzymes , leading to the conjugation of ISG 15 to cellular proteins . ^^^ |
|
| Interacting proteins: P05161 and P41226 |
Pubmed |
SVM Score :0.0 |
| For the ISG15ylation system , which is assumed to play an important role in interferon tau ( IFNT ) signaling , mRNAs of four potential components ( IFITM 1 , IFITM 3 , HSXIAPAF 1 , and DTX3L ) were found at increased levels in addition to ISG 15 and UBE1L . ^^^ |
|
| Interacting proteins: P05161 and P41226 |
Pubmed |
SVM Score :0.0 |
| Properties of the ISG 15 E1 enzyme UbE1L . ^^^ |
|
| Interacting proteins: P05161 and P41226 |
Pubmed |
SVM Score :0.0 |
| Like other known components of the protein ISGylation system ( ISG 15 , UBE1L , UBP 43 , and UBC 8 ) , EFP is also an interferon inducible protein . ^^^ |
|
| Interacting proteins: P05161 and P41226 |
Pubmed |
SVM Score :0.0 |
| To investigate the function of protein modification by ISG 15 , we generated a mouse model deficient in UBE1L , an ISG 15 activating enzyme . ^^^ Ube1L / mice did not produce ISG 15 conjugates but expressed free ISG 15 normally . ^^^ |
|
| Interacting proteins: P05161 and P41226 |
Pubmed |
SVM Score :0.0 |
| A recent proteomics study identified 158 human proteins targeted for ISG 15 conjugation , including the ISG 15 E1 and E 2 enzymes ( Ube1L and UbcH 8 , respectively ) and a HECT E 3 enzyme , Herc 5 . ^^^ Like the genes encoding Ube1L and UbcH 8 , expression of Herc 5 was also induced by IFN beta , suggesting that Herc 5 might be a component of the ISG 15 conjugation system . ^^^ In addition , co transfection of plasmids expressing ISG 15 , Ube1L , UbcH 8 , and Herc 5 resulted in robust ISG 15 conjugation in non IFN treated cells , while the active site cysteine mutant of Herc 5 or a mutant lacking the RCC 1 repeat region did not support ISG 15 conjugation . ^^^ |
|
| Interacting proteins: P05161 and P41226 |
Pubmed |
SVM Score :0.0 |
| Conversely , HERC 5 coexpression with Ube1L and UbcH 8 induces the ISG 15 conjugation in vivo independent of IFN stimulation . ^^^ |
|
| Interacting proteins: P05161 and P41226 |
Pubmed |
SVM Score :0.0 |
| In this study , we found that protein phosphatase 2Cbeta ( PP2Cbeta ) , which functions in the nuclear factor kappaB ( NF kappaB ) pathway via dephosphorylation of TGF beta activated kinase , was ISGylated , and analysis by NF kappaB luciferase reporter assay revealed that PP2Cbeta activity was suppressed by co expression of ISG 15 , UBE1L , and UbcH 8 . ^^^ In contrast to the wild type , this mutant suppressed the NF kappaB pathway even in the presence of ISG 15 , UBE1L , and UbcH 8 . ^^^ |
|
| Interacting proteins: P05161 and P41226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P05161 and P41226 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41226 and P05161 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41226 and P05161 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P41226 and P05161 |
Pubmed |
SVM Score :0.0 |
| We identify UBE1L as the E 1 enzyme that catalyzes the first activation step in the conjugation of ISG 15 , and show that the NS1B protein inhibits this activation step in vitro . ^^^ |
|
| Interacting proteins: P41226 and P05161 |
Pubmed |
SVM Score :0.0 |
| The increase in ISG 15 conjugates did not occur through a stabilization of the ISG 15 E1 enzyme , UBE1L . ^^^ |
|
| Interacting proteins: P41226 and P05161 |
Pubmed |
SVM Score :0.0 |
| The cap is subsequently removed with carboxypeptidase B to yield mature biologically active ISG 15 capable of stoichiometric ATP dependent thiolester formation with its human UbE1L activating enzyme . ^^^ The C terminal domain of ISG 15 was superimposed on the structure of Nedd 8 ( r . m . s . d . = 0 . 84 A ) bound to its AppBp 1 Uba3 activating enzyme to model ISG 15 binding to UbE1L . ^^^ |
|
| Interacting proteins: P41226 and P05161 |
Pubmed |
SVM Score :0.0 |
| Among the proteins induced by IFN alpha / beta are the ubiquitin like ISG 15 protein and its E 1 ( Ube1L ) and E 2 ( UbcH 8 ) conjugating enzymes , leading to the conjugation of ISG 15 to cellular proteins . ^^^ |
|
| Interacting proteins: P41226 and P05161 |
Pubmed |
SVM Score :0.0 |
| For the ISG15ylation system , which is assumed to play an important role in interferon tau ( IFNT ) signaling , mRNAs of four potential components ( IFITM 1 , IFITM 3 , HSXIAPAF 1 , and DTX3L ) were found at increased levels in addition to ISG 15 and UBE1L . ^^^ |
|
| Interacting proteins: P41226 and P05161 |
Pubmed |
SVM Score :0.0 |
| Properties of the ISG 15 E1 enzyme UbE1L . ^^^ |
|
| Interacting proteins: P41226 and P05161 |
Pubmed |
SVM Score :0.0 |
| Like other known components of the protein ISGylation system ( ISG 15 , UBE1L , UBP 43 , and UBC 8 ) , EFP is also an interferon inducible protein . ^^^ |
|
| Interacting proteins: P41226 and P05161 |
Pubmed |
SVM Score :0.0 |
| Involvement of UBE1L in ISG 15 conjugation during retinoid induced differentiation of acute promyelocytic leukemia . ^^^ RA treatment of APL and other RA responsive leukemic cells induced expression of UBE1L and ISG 15 as well as intracellular ISG 15 conjugates . ^^^ Induction of UBE1L and ISG 15 along with ISG 15 conjugation in RA sensitive NB 4 S1 APL cells were detected following treatment with specific retinoids and type 1 interferon ( IFN ) . ^^^ UBE1L and ISG 15 mRNAs were co expressed in normal human tissues that were examined . ^^^ A physical association was found between UBE1L and ISG 15 in vivo . ^^^ |
|
| Interacting proteins: P41226 and P05161 |
Pubmed |
SVM Score :0.0 |
| Isolation and sequence of an interferon tau inducible , pregnancy and bovine interferon stimulated gene product 15 ( ISG 15 ) specific , bovine ubiquitin activating E 1 like ( UBE1L ) enzyme . ^^^ Ubiquitin activating enzyme 1 like protein ( UBE1L ) initiates enzymatic conjugation by forming a thioester bond with ISG 15 , thus preparing it for transfer to the next series of enzymes . ^^^ Because bovUBE1L was purified through its interaction with rGST ISG 15 and shares significant amino acid and cDNA sequence identity with human UBE1L , it is concluded that it mediates conjugation of ISG 15 to uterine proteins in response to the developing and attaching conceptus . . ^^^ |
|
| Interacting proteins: P41226 and P05161 |
Pubmed |
SVM Score :0.0 |
| Like other components of the protein ISGylation system ( ISG 15 , UBE1L , and UBP 43 ) , Ubc 8 is an interferon inducible protein . ^^^ Furthermore , we established an assay system to detect ISGylated target proteins by cotransfection of ISG 15 , UBE1L , and Ubc 8 together with a target protein to be analyzed . ^^^ |
|
| Interacting proteins: P41226 and P05161 |
Pubmed |
SVM Score :0.0 |
| To investigate the function of protein modification by ISG 15 , we generated a mouse model deficient in UBE1L , an ISG 15 activating enzyme . ^^^ Ube1L / mice did not produce ISG 15 conjugates but expressed free ISG 15 normally . ^^^ |
|
| Interacting proteins: P41226 and P05161 |
Pubmed |
SVM Score :0.0 |
| A recent proteomics study identified 158 human proteins targeted for ISG 15 conjugation , including the ISG 15 E1 and E 2 enzymes ( Ube1L and UbcH 8 , respectively ) and a HECT E 3 enzyme , Herc 5 . ^^^ Like the genes encoding Ube1L and UbcH 8 , expression of Herc 5 was also induced by IFN beta , suggesting that Herc 5 might be a component of the ISG 15 conjugation system . ^^^ In addition , co transfection of plasmids expressing ISG 15 , Ube1L , UbcH 8 , and Herc 5 resulted in robust ISG 15 conjugation in non IFN treated cells , while the active site cysteine mutant of Herc 5 or a mutant lacking the RCC 1 repeat region did not support ISG 15 conjugation . ^^^ |
|
| Interacting proteins: P41226 and P05161 |
Pubmed |
SVM Score :0.0 |
| Conversely , HERC 5 coexpression with Ube1L and UbcH 8 induces the ISG 15 conjugation in vivo independent of IFN stimulation . ^^^ |
|
| Interacting proteins: P41226 and P05161 |
Pubmed |
SVM Score :0.0 |
| In this study , we found that protein phosphatase 2Cbeta ( PP2Cbeta ) , which functions in the nuclear factor kappaB ( NF kappaB ) pathway via dephosphorylation of TGF beta activated kinase , was ISGylated , and analysis by NF kappaB luciferase reporter assay revealed that PP2Cbeta activity was suppressed by co expression of ISG 15 , UBE1L , and UbcH 8 . ^^^ In contrast to the wild type , this mutant suppressed the NF kappaB pathway even in the presence of ISG 15 , UBE1L , and UbcH 8 . ^^^ |
|