| The yeast two hybrid assay , gluthione S transferase fusion protein pull down assay and immuno co precipitation assay indicated that the Q domain of TLE 1 is capable of binding gp 130 intracellular domain , and the intracellular membrane proximal region of gp 130 containing conserved Box 1 and Box 2 motifs seemed essential for this interaction . ^^^ The interaction between gp 130 and TLE 1 indicated that molecules of TLE family might play a role in gp 130 signaling . . ^^^ |