Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.51327988
Other cytokines oncostatin M ( OM ) , leukemia inhibitory factor ( LIF ) or ciliary neurotrophic factor ( CNTF ) also use the gp 130 transducer and induce its heterodimerization with LIF receptor ( LIFR ) . 0.51327988^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.54637847
Here we investigated gp 130 homodimerization and heterodimerization with the LIFR by fluorescence resonance energy transfer ( FRET ) and bimolecular fluorescence complementation ( BiFC ) . 0.54637847^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
This complex consists of a CNTF binding protein ( CNTF R ) and two proteins important for signal transduction [ gp 130 and leukaemia inhibitory factor receptor ( LIF R ) ] . ^^^ Biological activities of the protein preparations were determined by their ability to induce proliferation of BAF / 3 cells that were stably transfected with CNTF R , gp 130 and LIF R cDNAs . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
A 549 epithelial cells are subjected to growth inhibition only in the presence of OSM ( ED 50 = 6 ng / ml ) , even though they expressed LIF R and gp 130 transcripts . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
This report documents the mRNA expression profiles of LIF and the two components of the LIF receptor complex , LIF R and gp 130 , during early mouse embryogenesis . ^^^ High levels of LIF R and gp 130 mRNAs were observed in the deciduum , however . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
It is composed of a four helix bundle structure and shares the transmembrane signal transducing proteins , glycoprotein 130 ( gp 130 ) and leukemia inhibitory factor receptor ( LIF R ) . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
To continue these studies , we examined the mRNA expression of signal transducing molecule , gp 130 , and the LIF R as associated members of a receptor family . ^^^ The levels of gp 130 and LIF R mRNA expression had a maximum 2 3 fold increase over a 24 h period . ^^^ Injection of purified recombinant rat IL 6 ( rIL 6 ) had little effect on LIF R mRNA levels , but had a dramatic inducing effect on gp 130 mRNA levels . ^^^ Rats were also injected ( i . p . ) with Dexamethasone ( Dex ) and this had no effect on either gp 130 or LIF R mRNA level expression . ^^^ We found that the maximum IL 6R ( gp 80 ) , gp 130 and LIF R mRNA levels peaked at different times depending on the type of acute inflammation induced in the rat . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Each of these cytokines induces the hetero oligomerization of LIF R with gp 130 , a signal transducing subunit shared with interleukin 6 and interleukin 11 . ^^^ The introduction of mutations into human LIF that reduced the affinity for gp 130 while retaining affinity for LIF R has generated antagonists for LIF . ^^^ On cells that express LIF R and gp 130 , all LIF R ligands were antagonized . ^^^ Ligand triggered tyrosine phosphorylation of both LIF R and gp 130 was blocked by the antagonist . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
METHODS : Active transcription of LIF , IL 6 , and OSM , their related receptors ( LIF R , IL 6 R , and gp 130 ) , and the consecutive signal transducing molecules ( STAT 1 , STAT 3 , and STAT 5a ) were analyzed in reverse transcriptase polymerase chain reaction experiments . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
In addition , cytokines or their receptors not known to be expressed in RPE were included to widen our picture of cytokine gene expression in the eye : stem cell factor ( SCF ) , its receptor ( SCF R ) , low affinity nerve growth factor receptor p 75 ( p 75 ( NGF R ) , ciliary neutrothropic factor ( CNTF ) , and its receptor ( CNTF R ) , glycoprotein 130 interleukin 6 transducer ( gp 130 ( IL 6 SD ) , leukemia inhibitory factor ( LIF ) , and its receptor ( LIF R ) . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The propagation of pluripotential mouse embryonic stem ( ES ) cells is sustained by leukemia inhibitory factor ( LIF ) or related cytokines that act through a common receptor complex comprising the LIF receptor subunit ( LIF R ) and the signal transducer gp 130 . ^^^ However , the findings that embryos lacking LIF R or gp 130 can develop beyond gastrulation argue for the existence of an alternative pathway ( s ) governing the maintenance of pluripotency in vivo . ^^^ This activity , which we name ES cell renewal factor ( ESRF ) , is distinct from members of the IL 6 / LIF family because ( 1 ) it is effective on ES cells lacking LIF R ; ( 2 ) it is not blocked by anti gp 130 neutralizing antibodies ; and ( 3 ) it acts without activation of STAT 3 . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
We measured plasma levels of interleukin ( IL ) 1beta , IL 6 , tumor necrosis factor alpha ( TNF alpha ) , soluble forms of the cytokine receptor proteins gp 130 and leukemia inhibitory factor receptor ( LIF R ) , the anti inflammatory Clara cell 16 kD protein ( CC 16 ) , prolactin ( PRL ) , cortisol and 17beta estradiol in 21 anorexic women , 21 bulimic women and 21 healthy females . ^^^ As compared to healthy subjects , anorexics exhibited significantly increased plasma levels of gp 130 and LIF R , whereas bulimics had significantly decreased blood concentrations of CC 16 . ^^^ In anorexics , a significant negative correlation was found between plasma levels of gp 130 or LIF R and the body mass index . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The cytoplasmic domain of LIF R can generate the signals for growth arrest and differentiation of mouse myeloid leukemic cells when they are induced to form a homodimer of the cytoplasmic domain independently of gp 130 . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Here , we report the construction and expression of a CNTF / soluble CNTF receptor ( sCNTF R ) fusion protein ( Hyper CNTF ) with enhanced biological activity on cells expressing gp 130 and leukemia inhibitory factor receptor ( LIF R ) , but not membrane bound CNTF R . ^^^ Hyper CNTF efficiently induced dose dependent STAT 3 phosphorylation and proliferation of BAF 3 cells stably transfected with gp 130 and LIF R cDNAs . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Signal transduction by CNTF requires that it bind first to CNTF R , permitting the recruitment of gp 130 and LIF R , forming a tripartite receptor complex . ^^^ Cells that only express gp 130 and LIF R , but not CNTF R are refractory to stimulation by CNTF . ^^^ The proliferative results confirmed that CNTF required homodimerization of the gp 130 , CNTF R and LIF R receptor subunit whereas Hyper CNTF required heterodimerization of the gp 130 and LIF R receptor subunit . ^^^ We concluded that the fusion protein Hyper CNTF had superagonistic activity on target cells expressing gp 130 and LIF R , but lacking membrane bound CNTF R . . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Therefore , using the lithium pilocarpine status epilepticus model , we performed a detailed in situ hybridization time course study of five gp 130 cytokines ( interleukin [ IL ] 6 , leukemia inhibitory factor [ LIF ] , IL 11 , oncostatin m [ OSM ] , and ciliary neurotrophic factor ) , gp 130 , and the receptors of the cytokines we found to be induced ( IL 6 receptor [ IL 6R ] , LIF receptor [ LIF R ] , and IL 11 receptor [ IL 11R ] ) . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Signalling of leukaemia inhibitory factor ( LIF ) , ciliary neurotrophic factor ( CNTF ) , cardiotrophin 1 ( CT 1 ) , CT 1 like factor ( CLC ) or oncostatin M ( OSM ) through gp130 / LIF R is believed to be similar due to the presence of the common signal transducer gp 130 within the receptor complexes utilized , but the difference in the composition of gp130 / gp130 homodimers and gp130 / LIF R heterodimers is likely to be reflected in different signalling . ^^^ The inhibitory tyrosine motifs within the cytoplasmic parts of gp 130 and the LIF R act independently . ^^^ Whereas SHP 2 and SOCS 3 bind directly to the inhibitory motif of gp 130 , only SHP 2 was found to bind to the corresponding inhibitory sequence of the LIF R . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
To investigate gp 130 dimerization , we performed co precipitation experiments with the endogenous cytokine receptors gp 130 and leukemia inhibitory factor receptor ( LIF R ) and with gp 130 variants carrying two different C terminal peptide tags . ^^^ Confocal laser scanning microscopy was used for FRET detection in live cells . gp 130 and LIF R could be coprecipitated in the absence of ligand . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The IL 6 signal transducer , gp 130 , is also an affinity converter for the LIF receptor . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The LIF receptor is most related to the gp 130 signal transducing component of the IL 6 receptor , a feature that may provide a molecular basis for the intertwined biologies of LIF and IL 6 in the absence of obvious structural similarly between the ligands . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The LIF receptor is structurally related to the gp 130 signal transducing component of the IL 6 receptor and to the G CSF receptor . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The IL 6 signal transducer , gp 130 : an oncostatin M receptor and affinity converter for the LIF receptor . ^^^ The converter conferred high affinity binding of both LIF and OSM when expressed with the low affinity LIF receptor and is identical to the signal transducing subunit of the IL 6 receptor , gp 130 . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The LIF receptor is related to the gp 130 ' signal transducing ' component of the IL 6 receptor and to the G CSF receptor , with the transmembrane and cytoplasmic regions of the LIF receptor and gp 130 being most closely related . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The presence of leukaemia inhibitory factor ( LIF ) binding and expression of the gp 130 component of the LIF receptor were studied in the rabbit uterus during pregnancy . ^^^ The coexpression of LIF binding and gp 130 may indicate the presence of high affinity LIF receptor , which matches the pattern of LIF protein expression and , as in mice , suggests its importance for implantation . . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
OM was also suggested to bind to the LIF receptor ( LIF / OM receptor ) , which consists of a signal transducing subunit for LIF and IL 6 ( gp 130 ) and a LIF receptor alpha subunit . ^^^ PCR amplification clearly revealed high levels of gp 130 expression in AIDS KS cells , while the transcript of LIF receptor alpha subunit or IL 6 receptor alpha subunit was not observed . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
These data show that CT 1 has a wide range of hematopoietic , neuronal , and developmental activities and that it can act via the LIF receptor and the gp 130 signaling subunit . . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Previously it had been thought that the gp 130 alone is sufficient to bind ligand and transduce signal and that oncostatin M could bind to and activate the LIF receptor , but these data show this is not always the case . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
We also demonstrate a rapid and transient increase in Hck tyrosine kinase activity in parental ES cells stimulated by LIF and , finally , show that Hck is physically associated with gp 130 , an affinity converter and signal transducing component of the LIF receptor . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The beta receptor components for this cytokine family , gp 130 and LIF receptor beta , constitutively associate with Jak Tyk kinases . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Coexpression of the LIF receptor and gp 130 in these cells conferred high affinity LIF and OSM binding and responsiveness to LIF and OSM . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
To begin defining whether NG neurons were capable of responding to CNTF and LIF in vivo , Northern blots were used to examine expression of mRNAs coding for CNTF and LIF receptor components ( CNTFR alpha , gp 130 , and LIFR beta ) in the intact ganglion . mRNAs for all three receptor components were present in both E16 . 5 and newborn ganglia , suggesting that LIF and CNTF receptors are expressed in the NG in vivo . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Unlike the IL 6 receptor complex in which homodimerization of gp 130 appears to be critical for signal initiation , signaling by the CNTF and LIF receptor complexes depends on the heterodimerization of gp 130 with LIFR beta . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Using the reverse transcriptase polymerase chain reaction , we found that the human breast carcinoma MCF 7 cell line expressed the message for both LIF receptor and its signal transducing protein gp 130 , suggesting that these receptors might be biologically active . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Leukemia inhibitory factor , LIF receptor , and gp 130 in the mouse uterus during early pregnancy . ^^^ The present study was designed to investigate the changes of LIF protein , LIF receptor , and gp 130 in the mouse uterus during the early pregnancy . ^^^ LIF protein and LIF receptor were at high levels in the mouse uterus near the time of ovulation and on day 4 of pregnancy . gp 130 was highest on days 3 and 4 of pregnancy . ^^^ Both LIF receptor and gp 130 showed strong staining in the stroma of the day 5 uterus , at a time when LIF protein was low . ^^^ The presence of LIF receptor and gp 130 in the luminal epithelium on day 4 and in the stroma on day 5 may indicate the site of the high affinity LIF receptor . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Here , we describe the expression of LIF , related members of this group of cytokines , oncostatin M and ciliary neurotrophic factor , and the LIF receptor beta and glycoprotein gp 130 in normal human tissues and in the endometrium of fertile women . ^^^ The associated signal transducing component of the LIF receptor , gp 130 , is also expressed in both the luminal and glandular epithelium throughout the cycle . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Dexamethasone inhibited both basal and LIF induced ACTH secretion ( P < 0 . 05 ) and LIF induction of ACTH was also attenuated by immuneutralization of either the LIF receptor ( 35 % , P < 0 . 05 ) or the gp 130 affinity converter ( 41 % , P < 0 . 05 ) . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Interaction of the human IL 11R alpha with murine gp 130 was demonstrated : expression of the human IL 11R alpha in murine M 1 cells which constitutively express murine gp 130 ( and murine LIF receptor ) , resulted in the generation of specific high affinity binding sites for IL 11 ( Kd = 250 pM ) . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Residues in human leukemia inhibitory factor ( hLIF ) crucial for binding to both the human LIF receptor ( R ) and gp 130 were identified by analysis of alanine scanning mutants of hLIF in assays for both receptor binding and bioactivity . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Moreover , other members of the CNTF cytokine family that use gp 130 and / or LIF receptor beta as their signal transducing receptors LIF , oncostatin M , interleukin 6 , and interleukin 11 had similar effects on increasing ChAT activity and reducing 5HT expression in RN46A cells . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
IL 6 triggers homodimerization of gp 130 , whereas CNTF and LIF induce heterodimerization of gp 130 and LIF receptor . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
To define the specificity of the signal transduction by the LIF / OSM receptor ( a heterodimer of gp 130 and LIF receptor ( LIFR ) ) and the OSM specific receptor ( a heterodimer of gp 130 and OSM receptor ( OSMR ) ) , we reconstituted the receptor function by transfection into receptor negative Hep3B hepatoma cells . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Human OSM ( hOSM ) and LIF share a functional high affinity receptor that is composed of gp 130 and LIF receptor beta subunit ( LIFRbeta ) . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Human OSM is known to exhibit biological activities similar to LIF , because they share the same functional receptor composed of the LIF receptor and gp 130 . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
These responses are thought to be mediated through the functional LIF receptor ( LIFR ) , consisting of a heterodimeric complex of LIFR alpha chain ( LIFRalpha ) and gp 130 . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Leukemia inhibitory factor ( LIF ) is a hematopoietic cytokine which elicits its effects on diverse cell types via both gp 130 and a more specific LIF receptor . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Carcinoma cells constitutively express LIF mRNA , and 23 lines ( 92 . 0 % ) and all ( 100 % ) of 25 lines express LIF receptor mRNAs of LIFRbeta and gp 130 , respectively . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Leukaemia inhibitory factor ( LIF ) is a polyfunctional cytokine that is known to require at least two distinct receptor components ( LIF receptor alpha chain and gp 130 ) in order to form a high affinity , functional , receptor complex . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
In the present study we show that CT 1 binds to the KB epidermoid cancer cell surface through a tripartite receptor complex which includes the gp 130 signal transducing protein , LIF receptor beta ( LIFR beta ) and a third component displaying a molecular weight of 80 kDa . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Treatment of HepG 2 cells with CT 1 resulted in the induction of tyrosine phosphorylation of both transducing receptor subunits , gp 130 and LIF receptor , and this phosphorylation was completely inhibited by a neutralizing anti gp 130 mAb . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
LIF , CNTF , and CT 1 act through the known receptors LIF receptor beta ( LIFRbeta ) and gp 130 and well defined signaling pathways including receptor phosphorylation and STAT 3 activation . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The percentage of neurones surrounded by TH IR baskets within the L 3 and L 4 dorsal root ganglia following sciatic axotomy was significantly reduced in animals treated continuously for 2 weeks with a monoclonal antibody against the LIF receptor motif , gp 130 ( 0 . 833 mg ml 1 ) ( P < 0 . 05 , Mann Whitney U test ) . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Additionally , LIF enhances association of SHP 2 with the gp 130 subunit of the LIF receptor signaling complex . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Leukemia inhibitory factor ( LIF ) is a polyfunctional cytokine known to require at least two distinct receptor components ( LIF receptor alpha chain and gp 130 ) in order to form a high affinity , functional receptor complex . ^^^ Both forms of gp 130 bound LIF specifically and were capable of forming heterotrimeric complexes with soluble human LIF receptor alpha chain in the presence of human LIF . ^^^ In addition to the soluble forms of gp 130 , a soluble form of LIF receptor alpha chain was also detected in human urine and plasma . . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
LIF receptor gp 130 interaction investigated by homology modeling : implications for LIF binding . ^^^ The LIF signal transducing complex contains two receptor molecules , the LIF receptor ( LIFR ) and gp 130 . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
In NIH 3T3 cells ( which endogenously express gp 130 , LIF receptor beta [ LIFRbeta ] , and the protein product , c 12 , of the cDNA described here ) , mouse LIF , human LIF , and human OSM signaled through receptors containing the LIFRbeta and gp 130 but not through the mouse OSM receptor . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The LIF receptor complex is composed of a heterodimer of LIF receptor alpha ( LIFR alpha ) and gp 130 . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
We have expressed wild type human gp 130 or three mutants in murine myeloid M 1 UR21 cells that lack functional endogenous gp 130 but express the IL 6 receptor ( IL 6R ) and the LIF receptor ( LIFR ) . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The functional receptor for ciliary neurotrophic factor ( CNTF ) is comprised of a CNTF binding entity termed CNTF receptor alpha ( CNTFRalpha ) , and 2 signaling molecules called LIF receptor beta and gp 130 . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Leukaemia inhibitory factor ( LIF ) plays an essential role during cell differentiation and implantation in the mouse exerting its effects via the two dimerizing receptor subunits glycoprotein 130 ( gp 130 ) and LIF receptor beta ( LR beta ) . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
This pattern of specificity was accounted for by the finding that EF cells express gp 130 , but not the ligand binding subunit of the IL 6 receptor ( gp 80 ) nor the LIF receptor beta . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Two types of functional hOSM receptors are known : the type 1 OSM receptor is identical to the LIF receptor that consists of gp 130 and the LIF receptor beta subunit ( LIFRbeta ) , and the type 2 OSM receptor consists of gp 130 and the OSM receptor beta subunit ( OSMRbeta ) . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Leukemia inhibitory factor ( LIF ) induces growth arrest and macrophage differentiation of mouse myeloid leukemic cells through the functional LIF receptor ( LIFR ) , which comprises a heterodimeric complex of the LIFR subunit and gp 130 . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Leukaemia inhibitory factor ( LIF ) signals via a heterodimeric receptor complex comprised of the LIF receptor ( LIFR ) and the interleukin ( IL ) 6 signal transducer gp 130 . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Cell type specific responses to the leukemia inhibitory factor ( LIF ) / interleukin 6 cytokine family are mediated by dimerization of the LIF receptor alpha chain ( LIFRalpha ) with the signal transducer gp 130 or of two gp 130 molecules followed by activation of the JAK / STAT and Ras / mitogen activated protein kinase cascades . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
IL 6 ) , whereas CNTF and leukemia inhibitory factor ( LIF ) signal via a heterodimer of gp 130 and LIF receptor ( LIFR ) . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
LIF acts through a receptor complex composed of a low affinity LIF receptor ( LIFRbeta ) and gp 130 . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Therefore , in vivo and in vitro expression of LIF , IL 6 and their signal transducer genes encoding LIF receptor ( LIFR ) , IL 6 receptor ( IL 6R ) and gp 130 in human medulloblastoma cells were investigated by multiple cellular and molecular biology approaches . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
OSM and LIF can bind to the same functional receptor complex ( LIF receptor beta and gp 130 heteromultidimers ) and thus mediate overlapping spectra of biological activities . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
To investigate the role of CNTF / LIF signaling in regenerative responses of motoneurons , we studied the expression of the three receptor components , CNTF receptor alpha ( CNTFRalpha ) , LIF receptor beta ( LIFRbeta ) , and gp 130 , and the activation of the STAT 3 signal transduction pathway in the rat facial nucleus following peripheral nerve transection . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The two cytokines bind with high affinity to the same receptor ( LIF / OSM receptor ) , which consists of the LIF receptor alpha chain ( LIFRalpha ) and the signal transduction unit gp 130 . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
To generate type 1 or type 2 OSM receptors , gp 130 mutants were expressed together with either LIF receptor beta or OSM receptor beta . ^^^ The present study demonstrates that positions 189 192 of gp 130 cytokine binding domain are essential for OSM binding to both gp130 / LIF receptor beta and gp130 / OSM receptor beta heterocomplexes . . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The neurally active cytokine leukemia inhibitory factor ( LIF ) signals through a bipartite receptor complex composed of LIF receptor alpha ( LIFR ) and gp 130 . gp 130 and LIFR contain consensus binding motifs for the protein tyrosine phosphatase SHP 2 surrounding tyrosines 118 and 115 ( Y 118 and Y 115 ) of their cytoplasmic domains , respectively . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The receptor for leukemia inhibitory factor ( LIF ) consists of two polypeptides , the LIF receptor and gp 130 . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The LIF receptor shares a common gp 130 receptor subunit with the IL 6 cytokine superfamily . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Leukemia inhibitory factor ( LIF ) signals via the heterodimeric receptor complex comprising the LIF receptor alpha subunit ( LIFRalpha ) and the common signal transducing subunit for interleukin 6 cytokine receptors , gp 130 . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Furthermore , almost no phosphorylated STAT 3 ( signal transducer and activator of transcription 3 ) , downstream of the LIF receptor and the gp 130 signaling subunit , was observed in the ventricles of JVS and control mice . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The biological actions of interleukin 6 ( IL 6 ) , leukemia inhibitory factor ( LIF ) , and ciliary neurotrophic factor ( CNTF ) are mediated via respective functional receptor complexes consisting of a common signal transducing component , gp 130 , and other specific receptor components , IL 6 receptor alpha ( IL 6R ) , LIF receptor beta ( LIFR ) , and CNTF receptor alpha ( CNTFR ) . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
LIF , LIF receptor , gp 130 , as well as type 1 and 2 T helper cytokine expressions were investigated by reverse transcription polymerasse chain reaction , in situ hybridization , and histological studies . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Western blot analysis showed that bone marrow derived mast cells failed to express both LIF receptor ( LIFR ) and gp 130 , whereas 3T3 fibroblasts expressed both LIFR and gp 130 . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The pericryptal fibroblast cells expressed messenger RNA ( mRNA ) for interleukin 6 ( IL 6 ) , leukemia inhibitory factor ( LIF ) , LIF receptor alpha , and the common coreceptor glycoprotein 130 ( GP 130 ) , but not the IL 6 receptor alpha . ^^^ In comparison , normal colonic epithelial cells express mRNA for LIF but not IL 6 as well as the receptors for GP 130 , IL 6 receptor alpha but not LIF receptor alpha . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Binding of LIF to LIF receptor and gp 130 activates signal transduction pathways . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The present study demonstrates an upregulation of both the LIF receptor ( LIFR ) alpha subunit and the signal transducing subunit gp 130 following endothelial denudation of the carotid artery by balloon catheter . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Blockade of gp 130 or LIF receptor inhibited basal growth as well as CT 1 or ET 1 stimulated cardiac fibroblast growth . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Oncostatin M can activate two related receptors , one consisting of a low affinity LIF receptor subunit , LIFR beta , which forms a heterocomplex with the gp 130 signal transducing protein and can recognize both oncostatin M and LIF , and a second heterocomplex consisting of a subunit that specifically recognizes oncostatin M , OSMR beta , and the gp 130 protein . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Immunolocalization of gp 130 , IL 6R , and the LIF receptor showed that the glandular epithelium and also endothelial cells are targets for IL 6 and LIF . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
IL 6 receptor , LIF receptor and gp 130 were all expressed by cultured stromal and epithelial cells , showing that the lack of effect is not due to lack of expression of the receptor components . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The aim of this study was to assess the expression and regulation of LIF receptor ( LIFR ) and gp 130 mRNA in mouse uterus during early pregnancy using in situ hybridization and reverse transcription polymerase chain reaction ( RT PCR ) . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The neurokine leukemia inhibitory factor ( LIF ) initiates signaling through heterodimerization of the low affinity LIF receptor ( LIFR ) and gp 130 . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Upon binding LIF or cardiotrophin 1 , the LIF receptor alpha subunit ( LIFRalpha ) dimerizes with gp 130 , leading to activation of constitutively associated Jak 1 proteins and LIFRalpha gp 130 tyrosine phosphorylation . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Normal human pituitary tissue expressed LIF , as well as the LIF receptor ( LIFR ) signaling subunits , gp 130 and LIFR . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Leukemia inhibitory factor ( LIF ) , cardiotrophin 1 ( CT 1 ) , and oncostatin M ( OSM ) are four helix bundle cytokines acting through a common heterodimeric receptor composed of gp 130 and LIF receptor ( LIFR ) . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
We further demonstrate that lesion induces upregulation of CNTF related cytokines ( LIF , NNT 1 ) and , interestingly , a more pronounced upregulation of cytokine receptor components ( LIF receptor beta , gp 130 ) and TGFbeta in CNTF deficient animals . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
RT PCR and Western blot analysis was used in order to detect gp 130 , IL 6 receptor , LIF receptor or OSM receptor specific protein and mRNA in human adult cardiac myocytes and for detection of VEGF 1 specific mRNA in cardiac myocytes after incubation with OSM . ^^^ We detected high levels of mRNA for gp 130 and the OSM receptor in freshly isolated human cardiac myocytes but only low amounts of mRNA for the IL 6 receptor whereas mRNA for the LIF receptor was hardly detectable by RT PCR . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Results indicated that association of GPL to the intracellular portions of gp 130 , or LIF receptor , allowed the signaling cascade . . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Leukemia inhibitory factor ( LIF ) induced cell signaling occurs following sequential binding to the LIF receptor alpha chain ( LIFR ) , then to the gp 130 co receptor used by all members of the interleukin 6 family of cytokines . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
OB R is a single membrane spanning receptor most related to the gp 130 signal transducing component of the IL 6 receptor , the G CSF receptor , and the LIF receptor . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
While the expression of many of the markers was similar in mouse and human cells , significant differences were found in the expression of vimentin , beta 3 tubulin , alpha fetoprotein , eomesodermin , HEB , ARNT , and FoxD 3 as well as in the expression of the LIF receptor complex LIFR / IL6ST ( gp 130 ) . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
We show here that neural precursor cells isolated from embryonic rat cortices abundantly secrete leukemia inhibitory factor ( LIF ) and express its receptor components , gp 130 and LIF receptor . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
To date , more than 10 different four helix bundle ligands have been identified that incorporate gp 130 , or one of its close relatives such as LIF receptor , into functional oligomeric signaling complexes . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
LIF acts on cells by binding to the LIF receptor ( LIFR ) and gp 130 . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Gene activities of cytokines and their receptors interleukin ( IL ) 6 , leukemia inhibitory factor ( LIF ) , IL 11 and IL 6 receptor ( IL 6 R ) , LIF receptor and gp 130 relevant to implantation revealed a 17 beta estradiol ( E 2 ) mediated up regulation of IL 6 and an E 2 and P 4 mediated up regulation of IL 6 R in hTERT EECs . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Leukemia inhibitory factor ( LIF ) is a member of the gp 130 cytokine family and signals through the receptor complex of gp 130 and the LIF receptor ( LIFR ) to activate the JAK / STAT signaling cascade . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The receptor for leukemia inhibitory factor ( LIF ) consists of two polypeptides , the low affinity LIF receptor ( LIFR ) and gp 130 . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
LIF activates the JAK STAT 3 pathway through the class 1 cytokine receptor gp 130 , which forms a trimeric complex with LIF and the class 1 cytokine receptor LIF receptor beta . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Immunohistochemistry was used to assay LIF , LIF receptor , and gp 130 protein expression . ^^^ RESULT ( S ) : The expression of LIF , LIF receptor , and gp 130 messenger RNA and protein was increased in spontaneously differentiated HS 237 cells compared with undifferentiated cells , with high expression of an inhibitor of LIF mediated signaling , suppressor of cytokine signaling 1 , in undifferentiated hESCs . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
These cytokines initiate signaling by inducing either homodimerization of gp 130 or heterodimerization of gp 130 with leukemia inhibitory factor receptor beta components . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The receptor for granulocyte colony stimulating factor ( G CSFR ) and chimeric receptors consisting of the extracellular domain of G CSFR and the transmembrane and cytoplasmic domain of the leukemia inhibitory factor receptor , gp 130 , or c mpl function as homodimeric complexes . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Multiple regions within the cytoplasmic domains of the leukemia inhibitory factor receptor and gp 130 cooperate in signal transduction in hepatic and neuronal cells . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Interestingly , in leukemia inhibitory factor , another cytokine that signals through gp 130 , site 3 , is topologically conserved but has evolved to bind leukemia inhibitory factor receptor . . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Ciliary neurotrophic factor ( CNTF ) drives the sequential assembly of a receptor complex containing the ligand specific alpha receptor subunit ( CNTFR alpha ) and the signal transducers gp 130 and leukemia inhibitory factor receptor beta ( LIFR ) . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Ciliary neurotrophic factor ( CNTF ) associates with an alpha subunit ( CNTFRalpha ) of the receptor complex to initiate signal transduction by facilitating heterodimerization of the gp 130 transducing protein and the leukemia inhibitory factor receptor ( LIFR ) beta . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
A biologically active OSM receptor has been previously described that consists of a heterodimer of leukemia inhibitory factor receptor ( LIFR ) and gp 130 . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The structure of the receptor for this factor , OB R , is considerably similar to those of gp 130 , the common signal transducing receptor component for the interleukin 6 ( IL 6 ) family of cytokines , and leukemia inhibitory factor receptor ( LIFR ) . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Box 3 independent signaling mechanisms are involved in leukemia inhibitory factor receptor alpha and gp 130 mediated stimulation of mitogen activated protein kinase . ^^^ Chimeric receptors containing the entire or various cytoplasmic domains of either gp 130 or leukemia inhibitory factor receptor alpha ( LIFR ) were used to identify signaling molecules and regions of these polypeptides required for the stimulation of mitogen activated protein kinase ( MAPK ) . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Leukemia inhibitory factor action is mediated by a heterodimeric receptor consisting of two subunits , gp 130 and the low affinity leukemia inhibitory factor receptor ( LIFR ) . 2 . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
NNT 1 / BSF 3 induces tyrosine phosphorylation of glycoprotein 130 ( gp 130 ) , leukemia inhibitory factor receptor beta , and signal transducer and activator of transcription 3 in the SK N MC human neuroblastoma cells . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Serum concentrations of interleukin 6 ( IL 6 ) , IL 6 receptor ( IL 6R ) , gp 130 ( the IL 6 signaling protein ) , IL 1R antagonist ( IL 1RA ) and leukemia inhibitory factor receptor ( LIFR ) were assayed in 22 nonpregnant women and in 91 pregnant women before delivery and 1 and 3 days after delivery . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Using neutralizing antibodies to gp 130 , the OSM receptor ( OSMR ) , and the leukemia inhibitory factor receptor ( LIFR ) , we document that OSM exclusively uses the OSMR / gp 130 heterodimer in signaling events , rather than the LIFR / gp 130 heterodimer . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The Y 759 motif in gp 130 also attenuates gene induction mediated by the oncostatin M and leukemia inhibitory factor receptor complexes , which both contain gp 130 as the shared subunit . . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Its receptor complex consists of a non signaling alpha chain , CNTFR , and two signaling beta chains , gp 130 and the leukemia inhibitory factor receptor ( LIFR ) . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Involving of the cytoplasmic region of leukemia inhibitory factor receptor alpha subunit , IL 6 related signal transducer gp 130 or fas death domain for MAPK p42 / 44 activation in HL 60 cell with LIF or anti Fas IgG . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Its receptor complex consists of a ligand binding component , CNTF receptor ( CNTFR ) , associated with two signaling receptor components , gp 130 and leukemia inhibitory factor receptor ( LIFR ) . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
For signal transduction , CNTF requires a receptor complex , composed of the IL 6 signal transducing molecule gp 130 , leukemia inhibitory factor receptor ( LIFR ) beta , and CNTFR alpha . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Flow cytometric analyses detected both the OSM specific receptor and the gp 130 receptor at the chondrocyte cell surface , but failed to detect the leukemia inhibitory factor receptor ( LIFR ) . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Oncostatin M regulates the synthesis and turnover of gp 130 , leukemia inhibitory factor receptor alpha , and oncostatin M receptor beta by distinct mechanisms . ^^^ The cytokine receptor subunits gp 130 , leukemia inhibitory factor receptor alpha ( LIFRalpha ) , and oncostatin M receptor beta ( OSMRbeta ) transduce OSM signals that regulate gene expression and cell proliferation . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Using RT PCR we detected transcripts of the receptor components involved in OSM signaling , gp 130 , OSM receptor ( OSMR ) beta , and leukemia inhibitory factor receptor ( LIFR ) , in HCEC . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Signal of the cytoplasmic regions of leukemia inhibitory factor receptor ( LIFR ) alpha subunit and gp 130 involves Stat 3 activation in leukemic U 937 cells ] . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
We demonstrated expression of three components of the OSM receptor ; gp 130 , the leukemia inhibitory factor receptor ( LIFRbeta ) and the OSM specific receptor ( OSMRbeta ) . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The functional receptor complex of CNTF is composed of the CNTF receptor alpha ( CNTFR ) , gp 130 and the leukemia inhibitory factor receptor ( LIFR ) . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
A functional role of the membrane proximal extracellular domains of the signal transducer gp 130 in heterodimerization with the leukemia inhibitory factor receptor . gp 130 is the common signal transducing receptor subunit of interleukin ( IL ) 6 type cytokines . gp 130 either homodimerizes in response to IL 6 and IL 11 or forms heterodimers with the leukemia inhibitory factor ( LIF ) receptor ( LIFR ) in response to LIF , oncostatin M ( OSM ) , ciliary neurotrophic factor ( CNTF ) , cardiotrophin 1 ( CT 1 ) or cardiotrophin like cytokine resulting in the onset of cytoplasmic tyrosine phosphorylation cascades . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
METHODS AND RESULTS : This study was designed to determine left ventricular expression of CT 1 and its glycoprotein 130 ( gp 130 ) / leukemia inhibitory factor receptor complex in human end stage heart failure due to ischemic and dilated cardiomyopathy . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Novel neurotrophin 1 / B cell stimulating factor 3 ( NNT 1 / BSF 3 ) is a recently cloned gp 130 cytokine , acting through the tripartite ciliary neurotrophic factor receptor ( CNTFR ) alpha / leukemia inhibitory factor receptor ( LIFR ) / gp130 receptor complex . ^^^ Using RT PCR , expression of ciliary neurotrophic factor receptor alpha , leukemia inhibitory factor receptor , and gp 130 could be demonstrated in mRNA derived from murine corticotroph AtT 20 cells and murine pituitary tissue . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Human CNTF elicits cellular responses by induction of a receptor complex consisting of the CNTF alpha receptor ( CNTFR ) , which is not involved in signal transduction , and the beta receptors gp 130 and leukemia inhibitory factor receptor ( LIFR ) . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The functional receptor complex of ciliary neurotrophic factor ( CNTF ) , a member of the gp 130 family of cytokines , is composed of CNTF , the CNTF receptor alpha ( CNTFR ) , gp 130 , and the leukemia inhibitory factor receptor ( LIFR ) . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
A chimeric receptor ( 130 / 190 ) containing the cytoplasmic region of leukemia inhibitory factor receptor alpha subunit ( LIFRalpha , or gp 190 ) and the extracellular transmembrane region of gp 130 was generated . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
It acts through a membrane receptor complex comprising CNTF receptor alpha component ( CNTFRalpha ) , gp 130 , and leukemia inhibitory factor receptor to activate signal transducer and activator of transcription 3 signaling pathway . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
CNTF mediates its function by activating a tripartite receptor comprising the CNTF receptor alpha chain ( CNTFRalpha ) , the leukemia inhibitory factor receptor beta chain ( LIFRbeta ) and gp 130 . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Leukemia inhibitory factor receptor and gp 130 were expressed in neurons , and the ischemic damage of these proteins was rescued in the high LIF group . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Ciliary neurotrophic factor ( CNTF ) forms a functional receptor complex containing the CNTF receptor , gp 130 , and the leukemia inhibitory factor receptor ( LIFR ) . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Furthermore , although gp 130 and LIFR are able to bind independently to the CNTF / CNTFR sub complex , they never form homodimers but only heterodimers . ^^^ We propose that CNTF assembles a hexameric receptor complex composed of two CNTF , two CNTFR , one gp 130 and one LIFR molecule , and present a model of the reciprocal interaction of these molecules based on similarities with the IL 6 hexameric complex . . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Ciliary neurotrophic factor ( CNTF ) , leukemia inhibitory factor ( LIF ) , oncostatin M ( OSM ) , and interleukin 6 ( IL 6 ) compose a family of distantly related cytokines that initiate signaling by inducing either homodimerization of the `` beta ' ' signal transducing receptor component gp 130 ( in the case of IL 6 ) or heterodimerization between gp 130 and the gp 130 related LIFR beta ( in the case of CNTF , LIF , and OSM ) ; dimerization of beta receptor components in turn activates members of the Jak / Tyk family of receptor associated tyrosine kinases . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
By rational mutagenesis , receptor specific functional analysis , and visualization of complex formation in solution , we identified individual amino acid side chains involved specifically in the interaction of ciliary neurotrophic factor ( CNTF ) with CNTFR alpha and not with the beta components , gp 130 and LIFR . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The low affinity receptor for leukemia inhibitory factor ( LIFR ) interacts with gp 130 to induce an intracellular signal cascade . ^^^ The LIFR gp 130 heterodimer is implicated in the function of diverse systems . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The binding of 125I CNTF to sympathetic neurons involved all three components of the CNTF receptor complex , namely CNTFR alpha , LIFR , and gp 130 , as shown by cross linking experiments . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
In addition to including a CNTF specific component , known as CNTFR alpha , the CNTF receptor complex utilizes two receptor components , gp 130 and LIFR beta , that are shared with members of a family of broadly acting cytokines , including leukemia inhibitory factor ( LIF ) and interleukin 6 ( IL 6 ) . ^^^ CNTFR alpha does not play a direct role in signaling , but instead forms a complex with CNTF that promotes its binding to the signal transducing `` beta ' ' receptor components , gp 130 and LIFR beta . ^^^ CNTF first binds to CNTFR alpha , then recruits gp 130 , and finally complexes with LIFR beta . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Selection of particular substrates the transcriptional activator Stat 3 and protein tyrosine phosphatase PTP1D that characterize responses to the ciliary neurotrophic factor interleukin 6 cytokine family depended not on which Jak was activated , but was instead determined by specific tyrosine based motifs in the receptor components gp 130 and LIFR shared by these cytokines . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Here we show that the other CNTF receptor components , LIFR beta and gp 130 , are also expressed by skeletal muscle and that expression of all three CNTF receptor components is greatly increased in denervated muscle . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Cross linking experiments with iodinated factors were carried out on a cell line responsive to CNTF and LIF , as well as on COS cells that were cotransfected with various combinations of gp 130 , LIFR beta , and CNTF receptor ( CNTFR ) alpha , the previously cloned CNTF binding protein . ^^^ These experiments reveal that LIF cross links to LIFR beta alone , as well as to gp 130 when it is coexpressed with LIFR beta . ^^^ However , cross linking of CNTF to LIFR beta and gp 130 is only observed in the presence of CNTFR alpha . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The IL 6 induced gp 130 homodimer appears to be similar in function to the heterodimer formed between the leukemia inhibitory factor ( LIF ) receptor ( LIFR ) and gp 130 in response to the LIF or ciliary neurotrophic factor ( CNTF ) . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Other growth factor ligand and receptor transcripts were expressed in a stage specific manner : these included receptors for interleukin ( IL ) 6 ( IL 6R ) , leukemia inhibitory factor ( LIFR ) , tumor necrosis factor alpha ( TNF alpha ) ( TNFRp 80 and TNFRp 60 ) , and gp 130 . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
We hypothesize that the LIF / LIFR gp 130 system may be involved in the expansion and differentiation of the liver stem cell compartment . . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
In this study , in vivo and in vitro expression of LIF and its signal transducer genes , LIFR and gp 130 in medulloblastoma cells and its role ( s ) in the cell proliferation were investigated by different approaches . ^^^ The results revealed that 12 out of 13 cases examined were found to express LIF and more than 90 % of the samples to express LIFR and gp 130 . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The interleukin 6 ( IL 6 ) family of cytokines activates signaling through the formation of either gp 130 homodimers , as for IL 6 , or gp 130 leukemia inhibitory factor receptor ( LIFR ) heterodimers as for ciliary neurotrophic factor ( CNTF ) , leukemia inhibitory factor , oncostatinM , and cardiotrophin 1 . ^^^ IL 11 is a member of the IL 6 family and known to require gp 130 but not LIFR for signaling . ^^^ However , in contrast to the other two cytokines , IL 11 was unable to induce either gp 130 homodimerization or gp130 / LIFR heterodimerization . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Expression of leukaemia inhibitory factor receptor subunits LIFR beta and gp 130 in human oocytes and preimplantation embryos . ^^^ The expression of both components of the high affinity leukaemia inhibitory factor receptor , LIFR beta and glycoprotein 130 ( gp 130 ) , was investigated in human oocytes and individual in vitro cultured preimplantation embryos by reverse transcription polymerase chain reaction ( RT PCR ) . ^^^ Messenger RNA of both LIFR beta and gp 130 was detected in as little as 1 / 30 and 1 / 12 sample equivalents of cDNA respectively , in oocytes ( n = 4 ) , 4 cell and expanded , blastacyst stage embryos . ^^^ LIFR beta but not gp 130 transcripts were detected at the 2 , 8 and 10 cell stages , and in cavitating and hatched blastocysts . ^^^ In order to exclude a simian origin of these PCR products resulting from the Vero cell line that was used as a feeder during culture to the blastocyst stage , they were digested with restriction endonucleases Taql ( LIFR beta ) or Kpnl ( gp 130 ) . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Furthermore , receptor binding studies and functional studies reveal that CT 1 shares the signal transducing receptor components gp 130 and LIFR with the previously identified members of the IL 6 cytokine family . ^^^ CT 1 rapidly activates gp 130 and LIFR tyrosine phosphorylation in cultured cardiac myocytes . ^^^ The growth promoting effects of CT 1 therefore indicate that signaling pathways emanating from gp 130 and LIFR are coupled to cardiomyocyte hypertrophy . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Ciliary neurotrophic factor ( CNTF ) drives the sequential assembly of a receptor complex containing the ligand specific alpha receptor subunit ( CNTFR ) and the signal transducing beta subunits gp 130 and leukemia inhibitory factor receptor beta ( LIFR ) . ^^^ The membrane bound form mediates the neuronal actions of CNTF , whereas the soluble form serves to confer cytokine responsiveness to non neuronal cells expressing gp 130 and LIFR . ^^^ Two new types of CNTF variants , characterized by weakened interactions with either CNTFR or both LIFR and gp 130 , were developed , and the biological activities of these and other mutants were determined in non neuronal versus neuronal cells , as well as in non neuronal cells transfected with an expression vector for CNTFR . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Differential temporal expression of mRNAs for ciliary neurotrophic factor ( CNTF ) , leukemia inhibitory factor ( LIF ) , interleukin 6 ( IL 6 ) , and their receptors ( CNTFR alpha , LIFR beta , IL 6R alpha and gp 130 ) in injured peripheral nerves . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Crest derived cells , immunoselected from the fetal rat gut ( E 14 ) with antibodies to p75NTR , were found by RT PCR and immunocytochemistry ( after culture ) to express both alpha ( CNTER alpha ) and beta components ( gp 130 and LIFR beta ) of the tripartite CNTF receptor . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The functional receptor for LIF is composed of LIFR alpha chain ( LIFRalpha ) and gp 130 both of which are shared in the functional receptors for oncostatin M , ciliary neurotrophic factor , and cardiotrophin 1 . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Anti LIFR or anti gp 130 antibodies immunoprecipitated the 100 and the 190 kDa proteins . ^^^ CNTF induced the tyrosine phosphorylation of LIFR and gp 130 , as well as of proteins with the molecular weights of 88 / 91 and 42 kDa . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
OM signals are transduced through two different OM receptor complexes : a leukemia inhibitory factor ( LIF ) / OM receptor ( a heterodimer of LIFR beta and gp 130 ) and an OM specific receptor ( a heterodimer of OMR beta and gp 130 ) . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
We investigated the contributions of LIFR and OSMR to signal transduction in the context of heterodimers with gp 130 . ^^^ Our studies demonstrate that upon heterodimerization with the gp 130 cytoplasmic region , the cytoplasmic parts of both LIFR and OSMR were critical for activation of an acute phase protein promoter in HepG 2 hepatoma cells . ^^^ Membrane distal regions of LIFR and OSMR contributed to STAT activation even in the absence of gp 130 STAT recruitment sites . ^^^ We further show that the Janus kinases Jak 1 and Jak 2 constitutively associated with receptor constructs containing the cytoplasmic part of LIFR , OSMR , or gp 130 , respectively . ^^^ Thus , in spite of extensive functional similarities , differential signaling abilities of gp 130 , LIFR , and OSMR may become evident in a cell type specific manner . . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
IL 6 and IL 11 induce gp 130 homodimerization whereas the others lead to the formation of heterodimers with LIFR or OSMR . ^^^ Thus , epitopes involved in gp 130 homodimerization are distinct from those leading to the formation of gp130 / LIFR or gp130 / OSMR heterodimers . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Transcripts of the remaining two components of the CNTF receptor system , gp 130 and LIFR beta , were found by Northern blotting in 83 % and 70 % of the tumors , respectively . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Signal transduction by CNTF requires that it bind first to CNTFR alpha , permitting the recruitment of gp 130 and LIFR beta , forming a tripartite receptor complex . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Both mouse and human OSM engaged gp 130 with their respective OSMRbeta subunits , but only human OSM also acted through LIFR . ^^^ Since LIF together with LIFRalpha appear to form a more stable complex with gp 130 than OSM with gp 130 and OSMRbeta , co activation of LIFR and OSMR resulted in a predominant LIF like response . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Its mechanism of action is via specific binding to gp 130 and either the leukaemia inhibitory factor receptor ( LIFR ) or oncostatin M receptor ( OSMR ) systems at the cell surface to form an active signalling complex . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
All of these cell lines express the receptor components of OSM and leukemia inhibitory factor ( LIF ) gp 130 , LIFR and the OSM specific OSMRbeta . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
CNTF functional responses are mediated through a tripartite membrane receptor composed of two signalling receptor chains , gp 130 and the leukaemia inhibitory factor receptor ( LIFR ) , associated with a non signalling CNTF binding receptor alpha component ( CNTFR ) . ^^^ Co expression of CLC and CNTFR in mammalian cells generates a secreted composite cytokine , displaying activities on cells expressing the gp 130 LIFR complex on their surface . ^^^ Correspondingly , CLC CNTFR activates gp 130 , LIFR and STAT 3 signalling components , and enhances motor neuron survival . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Expression of mRNAs for ciliary neurotrophic factor ( CNTF ) , leukemia inhibitory factor ( LIF ) , interleukin 6 ( IL 6 ) , and their receptors ( CNTFR alpha , LIFR beta , IL 6R alpha , and gp 130 ) in human peripheral neuropathies . ^^^ The mRNA levels of neuropoietic cytokines , ciliary neurotrophic factor ( CNTF ) , leukemia inhibitory factor ( LIF ) , and interleukin 6 ( IL 6 ) , and their receptor components ( CNTFR alpha , LIFR beta , IL 6R alpha , and gp 130 ) were examined in seventy six patients with various peripheral neuropathies to determine the extent of expression of these cytokines and receptors , and their relationship to nerve fiber pathology and cell infiltration in the diseased nerves . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The mRNAs for LIF / LIFR , IL 6 / IL 6R , and their common signal transduction molecule , gp 130 , in olfactory nasal mucosa from control mice and from 3 day post OBX mice were detected with reverse transcriptase polymerase chain reaction ( RT PCR ) . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
An additional contact is postulated between the Ig like domain of gp 130 and the NH ( 2 ) terminal CBM of the LIFR . . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The heterodimeric cytokine composed of the soluble ciliary neurotrophic factor receptor ( sCNTFR ) and the IL 6 family member cardiotrophin like cytokine ( CLC ) was recently identified as a new ligand for gp 130 leukemia inhibitory factor receptor ( LIFR ) complex [ Plun Favreau , H . , Elson , G . , Chabbert , M . , Froger , J . , deLapeyriere , O . , Lelievre , E . , Guillet , C . , Hermann , J . , Gauchat , J . ^^^ CC FP shows enhanced activities in respect to the CLC / sCNTFR native complex , on a number of cells expressing gp 130 and LIFR on their surface . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Semiquantitative reverse transcription polymerase chain reaction ( RT PCR ) analysis showed that IL 11 and its receptor , CNTF and its receptor , LIFR , and gp 130 were constitutively expressed throughout the culture period . ^^^ Continuous treatment with Dex ( 10 ( 8 ) mol / L ) inhibited the endogenous production of IL 6 , LIF , OSM , IL 11R , and OSMR , but had no detectable effect on the expression of IL 11 , CT 1 , CNTF , CNTFR , LIFR , or gp 130 . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The basis for an interaction of IL 6 cytokines with adult sensory neurones has been established by analysing the levels and distribution of the two signal transducing receptor subunits , glycoprotein 130 ( gp 130 ) and leukaemia inhibitory factor receptor ( LIFR ) , in the dorsal root ganglion ( DRG ) of male adult rats before and following nerve injury . ^^^ The coexpression of gp 130 and LIFR in the same intracellular compartment following axotomy conveys potential responsiveness of injured sensory neurones to members of the IL 6 family of cytokines . . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
In order to investigate the effects of CNTF on fat cells , we examined the expression of CNTF receptor complex proteins ( LIFR , gp 130 , and CNTFRalpha ) during adipocyte differentiation and the effects of CNTF on STAT , Akt , and MAPK activation . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Here we studied the expression of cytokines IL 6 , LIF , IL 1beta and TNFalpha and cytokine receptors IL 6R , LIFR and Gp 130 in the rat brain after kainic acid induced status epilepticus using Northern blot analysis and in situ hybridization histochemistry . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Using the recently engineered designer cytokine IC 7 , which induces an active heterodimer of the LIFR and gp 130 after binding to the IL 6R , and several receptor chimeras of the LIFR and the interleukin 6 receptor ( IL 6R ) carrying the CBM of the IL 6R in place of the COOH terminal LIFR CBM , we could assign individual receptor subdomains to individual binding sites of the ligand . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
This PEG peptide conjugate was found to be both an antagonist of LIF LIFR binding and of LIF signaling in engineered Ba / F3 cells expressing LIFR and the gp 130 coreceptor . . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Since adipocytes express both gp 130 and LIFR proteins and are responsive to other IL 6 family cytokines , we examined the effects of CT 1 on 3T3 L 1 adipocytes . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
The mRNA levels of IL 6 , leukemia inhibitory factor ( LIF ) , ciliary neurotrophic factor ( CNTF ) and cardiotrophin 1 ( CT 1 ) as well as their receptor subunits , glycoprotein 130 ( gp 130 ) , IL 6 receptor ( IL 6R ) , LIFR , and CNTFR , were measured by semi quantitative RT PCR . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
CNTF activates membrane receptors ( CNTF Rs ) composed of one ligand specific binding subunit , defined CNTFR alpha , and two signal transducing subunits , termed leukaemia inhibitory factor receptor ( LIFR ) and gp 130 . ^^^ GT 1 7 cells were found to express CNTFR alpha , LIFR and gp 130 genes , as shown by reverse transcription polymerase chain reaction analysis , and the corresponding proteins , analysed by immunofluorescence and western blot . ^^^ CNTFR alpha , LIFR and gp 130 immunoreactive bands had an approximate size of 50 , 190 and 130 kDa , respectively . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
These effects of CT 1 were abolished in the presence of antibodies specific for gp 130 or LIFR and did not require RNA or protein synthesis . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Moreover , our results indicate that the cross talk among gp 130 cytokines is mediated by the ability of these cytokines to induce ligand dependent degradation of the LIFR , in a proteasome independent manner , which coincides with decreased levels of LIFR at the plasma membrane . ^^^ In summary , our results demonstrate that an inhibitory cross talk among specific gp 130 cytokines in 3T3 L 1 adipocytes occurs as a result of specific degradation of LIFR via a lysosome mediated pathway . . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
Here we have provided evidence that major cell types expressing theLIF receptor ( LIFR ) and its signal transducing partner gp 130 are mostly disparate in the mouse uterus during the glandular LIF expression in the morning of day 4 . ^^^ In contrast , LIFR and gp 130 expressions overlap in the luminal epithelium at the time of blastocyst attachment on the evening of day 4 when the second phase of LIF expression occurs in stromal cells surrounding the blastocyst , suggesting that LIF participates in implantation in a paracrine manner . ^^^ Similar expression patterns for LIFR and gp 130 were observed when a delayed implantation model was used . ^^^ For example , a transient overlapping expression of LIFR and gp 130 was evident at 12 h after estrogen induced termination of delayed implantation . ^^^ Coimmunoprecipitation experiments showed that LIFR and gp 130 form heterodimers and are available for LIF signaling at the time of blastocyst attachment . ^^^
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
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Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
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Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
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Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
NA
Interacting proteins: P42702 and P40189	Pubmed	SVM Score :0.0
NA