| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.68434516 |
| Structural requirements of the interleukin 6 signal transducer gp 130 for its interaction with Janus kinase 1 : the receptor is crucial for kinase activation . 0.68434516^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.7502543 |
| We conclude from these findings that Jak 1 , once bound to a gp 130 molecule , does not exchange between different receptors at the plasma membrane neither via the cytoplasmic compartment nor via a membrane associated state . . 0.7502543^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| Association of transcription factor APRF and protein kinase Jak 1 with the interleukin 6 signal transducer gp 130 . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| A single amino acid substitution ( Trp ( 666 ) > Ala ) in the interbox1 / 2 region of the interleukin 6 signal transducer gp 130 abrogates binding of JAK 1 , and dominantly impairs signal transduction . gp 130 is the common signal transducing receptor chain of interleukin ( IL ) 6 type cytokines . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| In the absence of JAK 1 , the phosphorylation of the gp 130 subunit of the IL 6 receptor and the activation of STATs 1 and 3 are greatly reduced . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| Interaction of IL 6 and IL 6 receptor induces homodimerization of gp 130 which interacts and activates an intracytoplasmic tyrosine kinase , JAK 1 kinase . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| Although JAK 2 was capable of associating with all three of the chimeras , JAK 1 co precipitated only with the gp 130 chimera . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| IL 6 also induced tyrosine phosphorylation of the signaling transducing subunit of the IL 6R in AF 10 cells , along with tyrosine phosphorylation of the gp 130 associated tyrosine protein kinase JAK 1 and the adaptor molecule p52shc . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| Analysis of the tyrosine phosphorylation events in gp 130 and Jak kinase family members revealed that B S 12 quickly induced the phosphorylation of gp 130 in a neural derived cell line , and that Jak 1 and Jak 2 were also recruited . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| METHODS AND RESULTS : Lysates from neonatal rat cardiac myocytes were immunoprecipitated with anti gp 130 , anti JAK 1 , or anti STAT 3 antibody and blotted with anti phosphotyrosine antibody . ^^^ Tyrosine phosphorylation of gp 130 , JAK 1 , and STAT 3 was observed after LIF stimulation in cardiac myocytes . ^^^ Furthermore , anti gp 130 antibody significantly inhibited the LIF induced activation of JAK 1 , STAT 3 , and MAPKs . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| IL 6 induced transient tyrosine phosphorylation of the IL 6 receptor signal transducing subunit gp 130 , the gp 130 associated protein tyrosine kinases Jak 1 , Jak 2 , and Tyk 2 , the phosphotyrosine phosphatase PTP1D / Syp , the adaptor protein Shc and the mitogen activated protein kinase Erk 2 , and accumulation of GTP bound p21ras . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| Mutations of STAT 5 that eliminated the interaction with JAK 1 reduced the activation of STAT 5 upon the gp 130 stimulation , although such mutants could be still activated through erythropoietin receptor . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| Because the requirement for Jak 1 is very similar to that previously shown for interleukin 6 signaling , the data support the concept that the G CSF receptor and gp 130 are both structurally and functionally similar . . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| LIF ( 10 ( 3 ) U / mL ) induced rapid tyrosine phosphorylation of gp 130 , JAK 1 , JAK 2 , STAT 1 , and STAT 3 but not Tyk 2 or STAT 2 . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| Upon stimulation with IL 6 / sIL 6R , the gp 130 , cytoplasmic Janus kinases JAK 1 and JAK 2 were tyrosine phosphorylated . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| The catalytic domain of the human Jak 2 was expressed and its specificity for phosphorylating peptide substrates derived from the gp 130 , STAT , and Jak 3 molecules was determined and compared to that for human Jak 1 and Jak3 . . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| Activation of the protein tyrosine phosphatase SHP 2 via the interleukin 6 signal transducing receptor protein gp 130 requires tyrosine kinase Jak 1 and limits acute phase protein expression . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| Both the phosphorylation of gp 130 and the association of gp 130 with JAK 1 and JAK 2 were increased after pressure overload . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| The key components involved are the signal transducing receptor subunit gp 130 , the Janus kinases Jak 1 , Jak 2 and Tyk 2 , STAT 1 and STAT 3 of the family of signal transducers and activators of transcription , the protein tyrosine phosphatase SHP 2 and the suppressors of cytokine signalling SOCS 1 , SOCS 2 and SOCS 3 . ^^^ The Janus kinases Jak 1 , Jak 2 , Tyk 2 and gp 130 show intermediate half lives . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| We further show that the Janus kinases Jak 1 and Jak 2 constitutively associated with receptor constructs containing the cytoplasmic part of LIFR , OSMR , or gp 130 , respectively . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| Both hIL 6 and vIL 6 activated gp 130 , Janus kinase 1 , signal transducers and activators of transcription 3 , and mitogen activated protein kinase in both MH 60 and B 9 cells . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| PTPepsilonC expression resulted in lower levels of IL 6 induced tyrosine phosphorylation of Jak 1 , Tyk 2 , gp 130 , and Stat 3 compared with parent cells . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| The signal transduction of gp 130 involves the Janus tyrosine kinases ( JAK ) JAK 1 , JAK 2 and Tyk 2 and then the downstream effectors comprising the signal transducer and activator of transcription 3 ( STAT 3 ) and mitogen activated protein kinase ( MAPK ) pathways . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| Further comparison of upstream signaling pathways revealed that JAK 1 was constitutively present in anti phosphotyrosine immunoprecipitates of IL 6 independent cells ; gp 130 was constitutively phosphorylated in a subset of IL 6 independent plasmacytomas , whereas other IL 6 independent lines showed no detectable gp 130 phosphorylation in the absence of exogenous IL 6 . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| The results obtained showed an activation of Janus kinases ( JAK 1 , JAK 2 , and TYK 2 ) leading to a tyrosine phosphorylation of the gp 130 and LIFR . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| Cell lysates were immunoprecipitated with agarose conjugated antiphosphotyrosine antibody and blotted with anti gp 130 , anti Jak 1 , or anti STAT 3 antibody . ^^^ Tyrosine phosphorylation of gp 130 , Jak 1 , and STAT 3 was observed after CNTF stimulation in these three cell lines . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| Here we show that the in vitro translated N terminal domains of Jak 1 are sufficient for binding to a biotinylated peptide comprising the membrane proximal 73 amino acids of gp 130 , the signal transducing receptor chain of interleukin 6 type cytokines . ^^^ Substitution of Tyr ( 107 ) to alanine , a residue conserved among Jaks and involved in hydrophobic core interactions of the proposed beta grasp domain , abrogated binding of full length Jak 1 to gp 130 in COS 7 transfectants . ^^^ By further mutagenesis we identified the loop 4 region of the Jak 1 beta grasp domain as essential for gp 130 association and gp 130 mediated signal transduction . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| A region encompassing the FERM domain of Jak 1 is necessary for binding to the cytokine receptor gp 130 . ^^^ To determine the role of this domain in governing recruitment of Jak 1 , but not Jak 3 , to the gp 130 subunit of the interleukin 6 family of cytokine receptors , the interaction of three Jak1 / Jak3 chimeras with gp 130 was investigated . ^^^ Chimeras 1 and 2 interacted with the cytoplasmic domain of gp 130 , although less efficiently than Jak 1 . ^^^ Only chimera 2 , however , restored gp 130 signalling in Jak 1 negative cells . ^^^ The data are consistent with recruitment of Jak 1 to gp 130 through the Jak 1 FERM domain , but also emphasise the likely requirement for precise Jak / receptor orientation to sustain function . . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| Inactivation to CNTF was not due to a decrease in CNTF receptor subunit gp 130 or in levels of Jak 1 or Jak 2 . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| The effects of interleukin 6 on key intermediates in early steps of the interleukin 6 signaling pathway , including janus kinase 1 , gp 130 , the interleukin 6 receptor , STAT 1 , and STAT 3 , were examined in both saline and endotoxin treated rats . ^^^ MEASUREMENTS AND MAIN RESULTS : In endotoxin treated rats , there was significant inhibition of interleukin 6 activation of janus kinase 1 , gp 130 , the interleukin 6 receptor , STAT 1 , and STAT 3 . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| Upon binding LIF or cardiotrophin 1 , the LIF receptor alpha subunit ( LIFRalpha ) dimerizes with gp 130 , leading to activation of constitutively associated Jak 1 proteins and LIFRalpha gp 130 tyrosine phosphorylation . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| Moreover , transactivation of gp 130 was critically dependent upon Janus kinase activation by the initiating receptor and correlated with rapid and sustained Janus kinase 1 and tyrosine kinase ( Tyk ) 2 tyrosine phosphorylation . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| Here we show that vIL 6 is N linked glycosylated at N 78 and N 89 and demonstrate that N linked glycosylation at site N 89 of vIL 6 markedly enhances binding to gp 130 , signaling through the JAK 1 STAT1 / 3 pathway and functions in a cytokine dependent cell proliferation bioassay . ^^^ Although hIL 6 is also N glycosylated at N 73 and multiply O glycosylated , neither N linked nor O linked glycosylation is necessary for IL 6 receptor alpha dependent binding to gp 130 or signaling through JAK 1 STAT1 / 3 . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| We detected gp 130 , JAK 1 and Ras but not STAT 3 and ERK 2 molecules in lipid rafts of NECs . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| Under basal conditions , the abundance of the IL 6 receptor , gp 130 , JAK 1 , STAT 1 , and STAT 3 proteins and levels of phosphorylation of ERK1 / 2 and p 38 were significantly increased in the liver in the 2 % casein group compared with the 20 % casein group . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| Further studies show that LMO 4 associates with gp 130 and Janus kinase 1 in several mammalian cell types . ^^^ |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P40189 and P23458 |
Pubmed |
SVM Score :0.0 |
| NA |
|