Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.94422521
While SOCS 3 binds with much higher affinity to a gp 130 phosphopeptide than to phosphopeptides derived from the other receptors , multiple SOCS 3 binding sites are predicted to exist in the leptin and erythropoietin receptors which may compensate for weaker binding to individual sites . . 0.94422521^^^
Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
Western blot analysis revealed an inhibition of LIF stimulated gp 130 and STAT 3 phosphorylation in SOCS 3 overexpressing AtT 20 cells . ^^^
Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
The key components involved are the signal transducing receptor subunit gp 130 , the Janus kinases Jak 1 , Jak 2 and Tyk 2 , STAT 1 and STAT 3 of the family of signal transducers and activators of transcription , the protein tyrosine phosphatase SHP 2 and the suppressors of cytokine signalling SOCS 1 , SOCS 2 and SOCS 3 . ^^^
Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
SOCS 3 exerts its inhibitory function on interleukin 6 signal transduction through the SHP 2 recruitment site of gp 130 . ^^^ Furthermore , we show that tyrosine 759 in gp 130 is essential for both SHP 2 and SOCS 3 but not for SOCS 1 to exert their inhibitory activities on interleukin 6 signal transduction . ^^^ Besides SHP 2 , SOCS 3 also interacts with the Tyr ( P ) 759 peptide of gp 130 . ^^^
Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
Suppressor of cytokine signaling 3 ( SOCS 3 ) is one member of a family of intracellular inhibitors of signaling pathways initiated by cytokines that use , among others , the common receptor subunit gp 130 . ^^^ The SH 2 domain of SOCS 3 has been shown to be essential for this inhibitory activity , and we have used a quantitative binding analysis of SOCS 3 to synthetic phosphopeptides to map the potential sites of interaction of SOCS 3 with different components of the gp 130 signaling pathway . ^^^ By contrast , phosphopeptides corresponding to other regions within gp 130 , Janus kinase , or signal transducer and activator of transcription proteins bound to SOCS 3 with weak or undetectable affinity . ^^^ The significance of pY 757 in gp 130 as a biologically relevant SOCS 3 docking site was investigated by using transfected 293T fibroblasts . ^^^ Although SOCS 3 inhibited signaling in cells transfected with a chimeric receptor containing the wild type gp 130 intracellular domain , inhibition was considerably impaired for a receptor carrying a Y > F point mutation at residue 757 . ^^^
Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
The endothelium of the brain capillaries also exhibited up regulation of both IL 6 receptor and gp 130 subunits during systemic inflammation , which allowed SOCS 3 expression in response to circulating IL 6 . ^^^
Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
The induction of SOCS 3 was closely correlated with STAT 3 phosphorylation , as well as the activation of an embryonic gene program , suggesting that cardiac gp 130 JAK signaling is precisely controlled by this endogenous suppressor . ^^^ We conclude that SOCS 3 is a mechanical stress inducible gene in cardiac muscle cells and that it directly modulates stress induced gp 130 cytokine receptor signaling as the key molecular switch for a negative feedback circuit for both myocyte hypertrophy and survival . . ^^^
Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
SHP 2 and SOCS 3 contribute to Tyr 759 dependent attenuation of interleukin 6 signaling through gp 130 . ^^^ We recently observed that the suppressor of cytokine signaling SOCS 3 also acts through the tyrosine motif 759 of gp 130 . ^^^ We show that receptor and membrane targeted SHP 2 counteracts IL 6 signaling independent of SOCS 3 binding to gp 130 . ^^^ On the other hand , SOCS 3 inhibits signaling in cells expressing a truncated SHP 2 protein , which is not recruited to gp 130 . ^^^ These data suggest , that there are two , largely distinct modes of negative regulation of gp 130 activity , despite the fact that both SOCS 3 and SHP 2 are recruited to the same site within gp130 . . ^^^
Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
Tyrosine 759 of gp 130 is required for the negative regulation of LIF mediated signaling by both the protein tyrosine phosphatase SHP 2 and the suppressor of cytokine signaling 3 ( SOCS 3 ) . ^^^ This indicates that STAT signaling is necessary for LIF and CNTF stimulated VIP and ChAT expression and Y 759 of gp 130 mediates the activities of SHP 2 and SOCS 3 , which act to negatively regulate STAT activity . . ^^^
Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
Two signalling regulators , suppressor of cytokine signalling 3 ( SOCS 3 ) and Src homology 2 domain containing tyrosine phosphatase 2 ( SHP 2 ) , are recruited to Y 757 following receptor activation ; however , the relative contribution made by each of these in down regulating gp 130 signalling is not known . ^^^ In the present study , we show the design of a mutant gp 130 receptor that can recruit SHP 2 , but not SOCS 3 . ^^^ Cells transfected with a chimaeric receptor containing the SHP 2 selective gp 130 intracellular domain showed an enhanced response to cytokine stimulation , which was similar to that shown by a chimaeric gp 130 receptor mutant carrying a Y757F point mutation that failed to recruit either SOCS 3 or SHP 2 . ^^^
Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
Dual in situ hybridization revealed that most corticotropes expressed IL 6 receptor and gp 130 mRNAs , and that 2 h after LPS treatment , SOCS 3 and c fos mRNAs were induced in corticotropes . ^^^
Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
In contrast to the IL 6 signal transducer gp 130 , which has been previously shown to recruit SOCS 3 to one of its phosphotyrosine residues ( Y 759 ) , peptide precipitation experiments suggest that SOCS 3 does not interact with phosphorylated tyrosine motifs of the IL 10R . ^^^
Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
These data suggest that desensitization of vascular cells to OnM is mediated in trans and involves Tyr residue 759 in gp 130 but is not mediated by either SOCS 3 or SHP 2 , the only two proteins currently known to bind to gp 130 at this site . . ^^^
Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
Gp 130 contains a crucial motif around tyrosine Y 759 , which mediates negative regulation through the feedback inhibitor SOCS 3 and the protein tyrosine phosphatase SHP 2 . ^^^ Whereas SHP 2 and SOCS 3 bind directly to the inhibitory motif of gp 130 , only SHP 2 was found to bind to the corresponding inhibitory sequence of the LIF R . ^^^
Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
Interestingly , the observed SOCS 3 phosphorylation does not require SOCS 3 recruitment to phosphotyrosine ( Tyr ( P ) ) 759 of gp 130 , and the kinetics of SOCS 3 phosphorylation do not match the activation kinetics of the Janus kinases . ^^^
Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
By performing a variety of preincubation studies and examining the ability of these cytokines to activate STATs , ERKs , and induce transcription of SOCS 3 mRNA , we have also examined the ability of gp 130 cytokines to modulate the action of their family members . ^^^
Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
Recent in vivo studies have highlighted the role of SOCS 3 in the negative regulation of signalling via gp 130 . ^^^ The absence of SOCS 3 enhanced JAK STAT and extracellular signal related kinase 1 / 2 ( ERK 1 / 2 ) mitogen activated protein kinase ( MAPK ) signal transduction via gp 130 , with higher levels of phosphorylated STAT 1 , STAT 3 , SH 2 domain containing cytoplasmic protein tyrosine phosphatase 2 ( SHP 2 ) , and ERK 1 / 2 in steady state and in response to LIF stimulation . ^^^
Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
In vivo studies have implicated SOCS 3 as a negative regulator of signaling downstream of gp 130 , the receptor subunit shared by IL 6 like cytokines . ^^^ Mice lacking SOCS 3 die at midgestation because of placental failure , and SOCS 3 ablation in a cell type specific manner results in changes in the functional outcome of gp 130 signaling in response to IL 6 . ^^^
Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
Binding of SOCS 3 to the phosphorylated Tyr ( 759 ) in the cytoplasmic region of gp 130 , the common signal transducing receptor chain of all IL 6 type cytokines , is necessary for inhibition of Janus kinase mediated signaling . ^^^ In the present study , we analyzed the effect of SOCS 3 on signal transduction by the proinflammatory cytokine oncostatin M ( OSM ) , which signals through a receptor complex of gp 130 and the OSM receptor ( OSMR ) . ^^^ We can show that the OSMR mediated signaling is inhibited by SOCS 3 to a similar extent as previously described for gp 130 . ^^^
Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
By contrast , negative regulation of HO 1 expression appeared to be mediated through the SH 2 domain containing tyrosine phosphatase 2 ( SHP 2 ) / suppressors of cytokine signaling 3 ( SOCS 3 ) binding site within the gp 130 IL 6 receptor subunit . ^^^
Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
Here we describe the solution structure of SOCS 3 , the first structure determined for any SOCS protein , in complex with a phosphotyrosine containing peptide from the IL 6 receptor signaling subunit gp 130 . ^^^
Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
Recently in Molecular Cell , Babon et al . ( 2006 ) described the tertiary structure of SOCS 3 in complex with a phosphotyrosine containing peptide from the IL 6 receptor subunit gp 130 , and they identified the specific amino acids that are critical for binding . . ^^^
Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
Here , we present a crystal structure at 2 . 0 A resolution of the N terminally extended SH 2 domain of SOCS 3 in complex with its phosphopeptide target on the cytokine receptor gp 130 . ^^^ The structure reveals that major insertions in the EF and BG loops of the SOCS 3 SH2 domain are responsible for binding to gp 130 with high affinity and specificity . ^^^
Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
This was associated with the induction of suppressor of cytokine signaling 3 ( SOCS 3 ) , a bona fide inhibitor in vivo of gp 130 , the signal transducing component of the IL 6 receptor complex . ^^^ Attenuation of SOCS 3 induction in either ECs or SOCS 3 null murine embryonic fibroblasts abolished the inhibitory effect of cAMP , whereas inhibition of SHP 2 , another negative regulator of gp 130 , was without effect . ^^^
Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
The actions of the neuropoietic cytokines are mediated by the gp 130 receptor , which activates several signaling molecules including the transcription factor STAT 3 ( signal transducer and activator of transcription ) , which , in turn , is subject to feedback inhibition by SOCS 3 ( suppressor of cytokine signaling ) . ^^^
Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
Prior activation of mitogen activated protein kinases by phorbol 13 myristate 12 acetate ( PMA ) results in an inhibition of interleukin ( IL ) 6 induced activation of the Janus kinase / signal transducer and activator of transcription ( STAT ) signaling pathway which is most likely mediated by the induction of suppressor of cytokine signaling 3 and requires the specific SHP 2 binding site Y 759 of the IL 6 signal transducer gp 130 . ^^^
Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
Inhibition of STAT activation was not dependent on tyrosine 759 of the IL 6 signal transducer gp 130 , suggesting that the inhibitory action of VSV M protein is not mediated by the induction of the suppressor of cytokine signaling 3 . ^^^
Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
Although gp 130 AS clones , which have low gp 130 levels and impaired signal transducer and activator of transcription 3 activity and suppressor of cytokine signaling 3 expression , showed reduced proliferation and hormone secretion ( GH and prolactin ) in response to gp 130 cytokines , they had a normal response to gp 130 independent stimuli . ^^^
Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
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Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
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Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
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Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
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Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
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Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
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Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
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Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
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Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
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Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
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Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
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Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
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Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
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Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
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Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
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Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
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Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
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Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
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Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
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Interacting proteins: O14543 and P40189	Pubmed	SVM Score :0.0
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