| Interacting proteins: P37231 and Q9Y6Q9 |
Pubmed |
SVM Score :0.8311426 |
| CONCLUSIONS : 1 ) FRET based coactivator association is a novel approach for characterizing nuclear receptor agonists or antagonists ; individual ligands display potencies that are predictive of in vivo effects and distinct profiles of maximal activity that are suggestive of alternative receptor conformations . 2 ) PPARgamma interacts with both CBP and SRC 1 ; transcriptional activation and coactivator association are AF 2 dependent . 3 ) Nuclear receptor LBDs have distinct affinities for individual coactivators ; thus , PPARgamma has a greater apparent affinity for CBP than for SRC 1 , whereas ERalpha interacts preferentially with SRC 1 but very weakly with CBP . . 0.8311426^^^ |
|
| Interacting proteins: P37231 and Q9Y6Q9 |
Pubmed |
SVM Score :0.56704745 |
| Steroid receptor coactivator 1 ( SRC 1 ) and PPAR binding protein ( PBP ) interact with PPARgamma and act as coactivators to enhance ligand dependent transcription . 0.56704745^^^ |
|
| Interacting proteins: P37231 and Q9Y6Q9 |
Pubmed |
SVM Score :0.0 |
| The peroxisome proliferator activated receptor gamma ( PPAR gamma ) is a ligand dependent transcription factor that is important in adipocyte differentiation and glucose homeostasis and which depends on interactions with co activators , including steroid receptor co activating factor 1 ( SRC 1 ) . ^^^ |
|
| Interacting proteins: P37231 and Q9Y6Q9 |
Pubmed |
SVM Score :0.0 |
| The docking of PGC 1 to peroxisome proliferator activated receptor gamma ( PPARgamma ) stimulates an apparent conformational change in PGC 1 that permits binding of SRC 1 and CBP / p300 , resulting in a large increase in transcriptional activity . ^^^ |
|
| Interacting proteins: P37231 and Q9Y6Q9 |
Pubmed |
SVM Score :0.0 |
| Using the yeast transactivator GAL 4 system , we showed that both AF 1 and AF 2 ( activation functions 1 and 2 ) of HNF 4 are inhibited by TNFalpha and that this inhibition was abolished by overexpression of different HNF 4 co activators , including PGC 1 ( peroxisome proliferator activated receptor gamma co activator 1 ) , CBP [ CREB ( cAMP response element binding protein ) binding protein ] and SRC 3 ( steroid receptor co activator 3 ) . ^^^ |
|
| Interacting proteins: P37231 and Q9Y6Q9 |
Pubmed |
SVM Score :0.0 |
| Overexpression of SRC 1 markedly potentiates ligand dependent transcription by PPARgamma , suggesting that SRC 1 serves as a coactivator in vivo . ^^^ |
|
| Interacting proteins: P37231 and Q9Y6Q9 |
Pubmed |
SVM Score :0.0 |
| In an attempt to identify cofactors that could possibly influence the transcriptional activity of peroxisome proliferator activated receptors ( PPARs ) , we used a yeast two hybrid system with Gal 4 PPARgamma as bait to screen a mouse liver cDNA library and have identified steroid receptor coactivator 1 ( SRC 1 ) as a PPAR transcriptional coactivator . ^^^ |
|
| Interacting proteins: P37231 and Q9Y6Q9 |
Pubmed |
SVM Score :0.0 |
| We previously isolated and identified steroid receptor coactivator 1 ( SRC 1 ) and peroxisome proliferator activated receptor ( PPAR ) binding protein ( PBP / PPARBP ) as coactivators for PPAR , using the ligand binding domain of PPARgamma as bait in a yeast two hybrid screening . ^^^ |
|
| Interacting proteins: P37231 and Q9Y6Q9 |
Pubmed |
SVM Score :0.0 |
| In white adipose tissue , lack of TIF 2 decreases PPARgamma activity and reduces fat accumulation , whereas in brown adipose tissue it facilitates the interaction between SRC 1 and PGC 1alpha , which induces PGC 1alpha ' s thermogenic activity . ^^^ |
|
| Interacting proteins: P37231 and Q9Y6Q9 |
Pubmed |
SVM Score :0.0 |
| YM 440 and rosiglitazone induced interaction between PPARgamma and the transcriptional cofactor , p 300 or SRC 1 , but YM 440 was 151 and 1091 fold less potent than rosiglitazone , respectively . ^^^ |
|
| Interacting proteins: P37231 and Q9Y6Q9 |
Pubmed |
SVM Score :0.0 |
| These showed that the indomethacin , a PPAR gamma ligand , increased the binding between PPAR gamma and SRC 1 in a ligand dose dependent manner . ^^^ These results suggested that the in vitro conformational change of PPAR gamma by ligands was also induced , and increased the levels of the ligand dependent interaction with SRC 1 . ^^^ This screening system ( based on the interaction between PPAR gamma and SRC 1 ) may be a promising system in the development of drugs for metabolic disorders . . ^^^ |
|
| Interacting proteins: P37231 and Q9Y6Q9 |
Pubmed |
SVM Score :0.0 |
| We demonstrate that while RA does not activate PPARalpha and PPARgamma , it binds to PPARbeta / delta with nanomolar affinity , modulates the conformation of the receptor , promotes interaction with the coactivator SRC 1 , and efficiently activates PPARbeta / delta mediated transcription . ^^^ |
|
| Interacting proteins: P37231 and Q9Y6Q9 |
Pubmed |
SVM Score :0.0 |
| CBP and p 300 are thought to be recruited to nuclear receptors through bridging factors that include SRC 1 , although CBP also interacts directly with PPARgamma through its amino terminus . ^^^ We here provide evidence that PPARgamma ' s ability to repress iNOS transcription requires the ligand dependent charge clamp that mediates interactions with CBP and SRC 1 . ^^^ Single amino acid mutations in PPARgamma that abolished ligand dependent interactions with SRC 1 and CBP not only resulted in complete loss of transactivation activity but also abolished transrepression . ^^^ Conversely , a CBP deletion mutant containing the SRC 1 interaction domain but lacking the N terminal PPARgamma interaction domain was inactive as a PPARgamma coactivator and failed to rescue transrepression . ^^^ Together , these findings are consistent with a model in which transrepression by PPARgamma is achieved by targeting CBP through direct interaction with its N terminal domain and via SRC 1 like bridge factors . . ^^^ |
|
| Interacting proteins: P37231 and Q9Y6Q9 |
Pubmed |
SVM Score :0.0 |
| Members of the 160 kDa protein ( SRC 1 / TIF2 / AIB 1 ) family of coactivators , CBP / p300 and TRAP220 / DRIP205 , are shown to interact directly with PPARgamma and potentiate nuclear receptor transactivation function in a ligand dependent fashion . ^^^ The natural ligand 15 deoxy Delta 12 , 14 prostaglandin J ( 2 ) induced PPARgamma interactions with all coactivators tested ( SRC 1 , TIF 2 , AIB 1 , p 300 , TRAP220 / DRIP205 ) in yeast and mammalian two hybrid assays , as well as in a glutathione S transferase pull down assay . ^^^ |
|
| Interacting proteins: P37231 and Q9Y6Q9 |
Pubmed |
SVM Score :0.0 |
| Interestingly , fatty acyl CoAs , unlike fatty acids , failed to recruit steroid receptor co activator 1 ( SRC 1 ) , on the basis of conformational changes in the LBDs of PPARalpha and PPARgamma . ^^^ |
|
| Interacting proteins: P37231 and Q9Y6Q9 |
Pubmed |
SVM Score :0.0 |
| Peroxisome proliferator activated recetor gamma ( PPARgamma ) and its coactivators , PGC 1alpha and SRC 1 , influence brown adipocyte metabolism and development . ^^^ |
|
| Interacting proteins: P37231 and Q9Y6Q9 |
Pubmed |
SVM Score :0.0 |
| Moreover , LPS administration also decreased the expression of the coactivators CREB binding protein ( CBP ) / p300 , steroid receptor coactivator ( SRC ) 1 , SRC 3 , TR associated protein ( TRAP ) 220 , and PPARgamma coactivator ( PGC ) 1 , all of which are required for the transcriptional activity of RXR PPAR and RXR TR . ^^^ |
|
| Interacting proteins: P37231 and Q9Y6Q9 |
Pubmed |
SVM Score :0.0 |
| In mammalian two hybrid assays , DIM C pPhCF ( 3 ) and PGJ 2 ( 5 20 micro M ) induced interactions of PPARgamma with steroid receptor coactivator ( SRC ) 1 , SRC 2 ( TIFII ) , and thyroid hormone receptor associated protein 220 but not with SRC 3 ( AIB 1 ) . ^^^ |
|
| Interacting proteins: P37231 and Q9Y6Q9 |
Pubmed |
SVM Score :0.0 |
| An in vitro binding assay revealed that PPARgamma 2 was bound to steroid receptor coactivator 1 ( SRC 1 ) in a dose responsive manner in the presence of indomethacin , and Pr48 . 34A was able to detect PPARgamma in a complex consisting of PPARgamma and SRC 1 . ^^^ Using Pgamma48 . 34A antibody , an enzyme linked immunosorbent assay ( ELISA ) system based on the binding between fPPARgamma 2 and SRC 1 has been optimized to screen new PPARgamma ligands . ^^^ |
|
| Interacting proteins: P37231 and Q9Y6Q9 |
Pubmed |
SVM Score :0.0 |
| Based on the binding between peroxisome proliferators activated receptor gamma ( PPARgamma ) and steroid receptor co activator 1 ( SRC 1 ) , an enzyme linked immunosorbent assay ( ELISA ) was used to screen new PPARgamma ligands from various benzylidinethiazole derivatives , which have anti inflammatory activity . ^^^ Among those derivatives , 5 ( 3 , 5 di tert butyl 4 hydroxybenzylidene ) thiazolidine 2 , 4 dione ( BTZD ) increased the binding between PPARgamma and SRC 1 . ^^^ BTZD was found to induce adipogenesis and PPARgamma trans activation in 3T3 L 1 pre adipocyte , and increased the binding between PPARgamma and SRC 1 in in vitro binding assay and complex consisting of PPARgamma and SRC 1 in the co immunoprecipitaion . ^^^ Chromatin immunoprecipitation ( ChIP ) analysis revealed that BTZD induced the binding of PPARgamma SRC 1 complex to PPAR response element ( PPRE ) in the same pattern of other PPARgamma ligand . ^^^ |
|
| Interacting proteins: P37231 and Q9Y6Q9 |
Pubmed |
SVM Score :0.0 |
| The amount of CBP ( CREB binding protein ) and SRC 1 ( steroid receptor coactivator 1 ) recruited by FK 614 was less than that induced by rosiglitazone and pioglitazone , but FK 614 caused similar PGC 1alpha ( PPARgamma coactivator 1alpha ) recruitment as these compounds . ^^^ |
|
| Interacting proteins: P37231 and Q9Y6Q9 |
Pubmed |
SVM Score :0.0 |
| We report here that a developmental arrest in interscapular brown fat and defective adaptive thermogenesis occur in mice lacking both the p 160 family transcriptional coactivators SRC 1 and p / CIP due to a failure in induction of selective PPARgamma target genes involved in adipogenesis and mitochondrial uncoupling . ^^^ |
|
| Interacting proteins: P37231 and Q9Y6Q9 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37231 and Q9Y6Q9 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37231 and Q9Y6Q9 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37231 and Q9Y6Q9 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37231 and Q9Y6Q9 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37231 and Q9Y6Q9 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37231 and Q9Y6Q9 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37231 and Q9Y6Q9 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37231 and Q9Y6Q9 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37231 and Q9Y6Q9 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P37231 and Q9Y6Q9 |
Pubmed |
SVM Score :0.0 |
| NA |
|