| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| We analyzed the mRNA expression of TGF beta 1 , TGF beta 2 , TGF beta 3 , the TGF beta receptors type 1 ( TbetaR 1 ) and type 2 ( TbetaR 2 ) , Smad 2 , Smad 3 , and Smad 4 . mRNA expression of IL 10 and CD 95 ( FAS / APO 1 ) were also studied . ^^^ |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| METHODS : Paraffin embedded tissue sections containing tumor and adjacent nonneoplastic parenchyma were analyzed by immunohistochemistry for the expression of TbetaR type 2 ( TbetaR 2 ) and Smad 2 , Smad 4 , Smad 6 , and Smad 7 . ^^^ |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| To learn about the role of TGF beta in vivo , phenotypes of targeted mutations of molecules within the TGF beta signalling pathway , TGF beta 1 , beta 2 , beta 3 , TGF beta receptor ( TbetaR 2 ) and the signalling molecules SMAD 2 , SMAD 3 and SMAD 4 , are discussed in this review . ^^^ |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| OBJECTIVE : To explore the relationships between Smad 4 , TGF beta 1 and TbetaR 2 in the TGF beta pathway and the possible mechanisms by which they effect pancreatic carcinoma . ^^^ METHODS : The expression of Smad 4 , TGF beta 1 and TbetaR 2 in paraffin embedded pancreatic carcinoma tissues was detected by using antibodies against Smad 4 , TGF beta 1 and TbetaR 2 with EnVision immunohistochemistry . ^^^ RESULTS : The positive rates of Smad 4 , TGF beta 1 and TbetaR 2 were 58 . 93 % ( 33 ) , 66 . 07 % ( 37 ) and 60 . 71 % ( 34 ) , respectively . ^^^ CONCLUSIONS : The expression of Smad 4 in pancreatic carcinoma tissue is significantly decreased but the expression of TGF beta 1 and TbetaR 2 are increased . ^^^ |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| In some cancers , TGF beta resistance has been linked to TGF beta receptor 2 ( TbetaR 2 ) and Smad 4 mutations ; however , in ovarian cancer , the mechanism of resistance remains unclear . ^^^ |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| To investigate the potential association between loss of sensitivity to TGF beta and expression status of transforming growth factor receptor 2 ( TbetaR 2 ) , Smad 4 , CDC25A and C myc in 14 cell lines derived from ovarian cancer , the expression levels of these genes were detected by semi quantitative RT PCR . ^^^ |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| METHODS : The expression of E 7 , TGF beta 1 , TbetaR 2 and Smad 4 was detected by immunohistochemistry in paraffin embedded cervical samples , and by RT PCR and Western blotting in cervical cancer cell lines . ^^^ |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| Using transient expression of TGF beta signalling components and a Smad 3 dependent reporter assay , we show that BICR 31 and H 314 had defects of Smad 4 , BICR 56 had abnormal TbetaR 2 and BICR 19 overexpressed Smad 7 . ^^^ |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| We showed that restoration of TGF beta induced transcriptional responses by Smad 4 was inhibited by co transfection with a kinase dead TGF beta type 2 receptor and that constitutive activation was blocked with TGF beta neutralizing antibodies , confirming the essential role of Smad 4 in TGF beta signaling . ^^^ |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| We have shown that TGF beta induces p21waf1 expression in Panc 1 cells , whereas no induction of p21waf1 expression by TGF beta was detected in the other four cell lines lacking either DPC 4 expression or the TGF beta type 2 receptor . ^^^ |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| Co transfection into SW480 . 7 cells of Smad 4 together with a Ras phosphorylation resistant Smad 3 ( but not with wild type Smad 2 , Smad 3 , adenomatous polyposis coli ( APC ) , or TGF beta type 2 receptor ) restored the TGF beta antiproliferative response . ^^^ |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| Genetic analysis disclosed a nonsense mutation in the Mad homologue 2a domain of the Smad 4 gene in the SK ChA 1 cells and a heterozygous deletion in the TGF beta type 2 receptor gene in the KMBC cells . ^^^ |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| We then analyzed TGF beta type 2 receptor ( T beta RII ) gene , immunoblots of Smad 4 , and restoration of the pathway by rescuing T beta R or Smad . ^^^ |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| These genes were TGF beta type 1 receptor gene ( TGFBR 1 ) , TGF beta type 2 receptor gene ( TGFBR 2 ) , SMAD 2 gene ( SMAD 2 ) , SMAD 3 gene ( SMAD 3 ) , SMAD 4 gene ( SMAD 4 ) , and SMAD 7 gene ( SMAD 7 ) , all of which compose the TGF beta 1 signaling pathway . ^^^ |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| All primary antibodies ( polyclonal antibodies ) to TGF beta 1 , type 2 Transforming growth factor beta receptor ( TbetaR 2 ) , nuclear factor kappaB ( NF kappaB ) , CD 34 , smad 4 and smad 7 , secondary antibodies and immunohistochemical kit were purchased from Zhongshan Biotechnology Limited Company ( Beijing , China ) . ^^^ The expressions of TGF beta 1 , TbetaR 2 , NF kappaB , smad 4 and smad 7 proteins in 36 specimens of hepatocellular carcinoma ( HCC ) and its adjacent tissue were separately detected by immunohistochemistry to observe the relationship between TGF beta 1 and TbetaR 2 , between NF kappaB and TGF beta 1 , between smad 4 and smad 7 and between TGF beta 1 or TbetaR IIand microvessel density ( MVD ) . ^^^ CONCLUSION : The expressions of TGF beta 1 , TbetaR 2 , NF kappaB , smad 4 and smad 7 in HCC tissue , which are major up and down stream factors of TGF beta 1 smad signal transduction pathway , are abnormal . ^^^ The inhibitory action of TGF beta 1 is weakened in hepatic carcinoma cells because of abnormality of TGF beta 1 receptors ( such as TbetaR 2 ) and postreceptors ( such as smad 4 and smad 7 ) . ^^^ |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| The human pancreatic ductal adenocarcinoma cell line , UK Pan 1 , which expresses DPC 4 , is not highly responsive to TGF beta due to transcriptional repression of TGF beta type 2 receptor ( RII ) . ^^^ |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| Mutational analysis of TGF beta type 2 receptor , Smad 2 , Smad 3 , Smad 4 , Smad 6 and Smad 7 genes in colorectal cancer . ^^^ Then , all coding regions of the TGF beta type 2 receptor ( TRII ) and the genes for Smad 2 , Smad 3 , Smad 4 , Smad 6 , and Smad 7 were analyzed by PCR SSCP and direct sequencing . ^^^ |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| Using the whole cell recording mode of the patch clamp technique , functional ion channels were electrophysiologically characterized in PANC 1 ( K ras G12D ( + / ) , p 53 R273C , Deltap 16 ) , BxPC 3 ( smad 4 , p 53 Y220C , Deltap 16 ) , and MiaPaCa 2 [ transforming growth factor beta receptor type 2 defect , K ras G12C ( / ) , p 53 R248W , Deltap 16 ] human pancreatic cancer cell lines . ^^^ |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| The aim of our study was to determine the presence of alterations in the main putative components of the TGF beta inhibitory signaling pathways ( p 15 , Smad 4 , Smad 2 , TGFbeta RII , CDC25A ) . ^^^ |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| Eighty two % of these pancreatic cancers had genetic inactivation of the DPC 4 , p 15 , ALK 5 , or TGFBR 2 genes . ^^^ |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| Genetic alterations in the TGFBR 2 gene but not in the MADH 4 gene . ^^^ |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| We report results of an association study between BMD and nine candidate genes ( TGFB 1 , TGFBR 2 , SMAD 2 , SMAD 3 , SMAD 4 , IFNB 1 , IFNAR 1 , FOS and LRP 5 ) , as well as of a case control study of osteoporosis . ^^^ |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P37173 and Q13485 |
Pubmed |
SVM Score :0.0 |
| NA |
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