Pubmed abstracts for Protein-Protein Interaction search result :

Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.57876329
FLT 3 is a novel cytokine receptor with intrinsic ligand stimulated ( FLT 3 ligand , FL ) tyrosine kinase activity . 0.57876329^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.5230046
FLT 3 was highly phosphorylated in these cell lines with FLT 3 D835 mutations , leading to constitutive activation of downstream targets such as signal transducer and activator of transcription 5 ( STAT 5 ) without FLT 3 ligand stimulation . 0.5230046^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In this study we examined whether expansion of dendritic cells ( DC ) by Flt 3 ligand ( Flt3L ) treatment influences the induction of immune responses following transcutaneous immunization . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The cytokine Flt 3 ligand ( Flt3L ) also has been shown to generate BM DCs . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
CD34+ cells from cord blood ( CB ) , bone marrow ( BM ) , or peripheral blood ( PB ) were expanded in a medium containing stem cell factor ( SCF ) , flt 3 ligand ( Flt3L ) and thrombopoietin ( TPO ) with or without HESS 5 , a murine BM stromal cell line , for 2 weeks ( the first expansion step ) , then differentiated to DC in a medium containing granulocyte macrophage colony stimulating factor ( GM CSF ) , flt 3 ligand ( Flt3L ) , stem cell factor ( SCF ) , tumor necrosis factor alpha ( TNF alpha ) , IL 4 , and lipopolysaccharide ( LPS ) for 9 days ( the second differentiation step ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Delivery of Flt 3 ligand ( Flt3L ) using a poloxamer based formulation increases biological activity in mice . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand ( Flt3L ) is a growth factor for hemopoietic progenitors and can promote the expansion of both conventional dendritic cells ( DCs ) and plasmacytoid predendritic cells ( p preDCs ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Upon in vivo Flt 3 ligand ( Flt3L ) administration , Flt3+ progenitor cells and their progeny DCs are expanded , whereas Flt 3 downstream progenitors are not , or are only slightly increased . ^^^ These findings provide a definitive map of Flt 3 expression in the hematopoietic hierarchy and directly demonstrate that Flt3L can drive DC development along both the lymphoid and myeloid developmental pathways from Flt3+ progenitors to Flt3+ DCs . . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
This report describes age related alterations of dendritic cells ( DC ) distribution in nude athymic mice in vivo and reversal of certain age dependent defects by an in vivo administration of hematopoietic growth factor FLT 3 ligand ( FLT3L ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
OBJECTIVE : We investigated the effect of interleukin 15 ( IL 15 ) with Flt 3 ligand ( Flt3L ) on the expansion and activation of NK cells derived from umbilical cord blood mononuclear cells ( UCB MNCs ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Indeed , flt 3 ligand ( flt3L ) treatment of athymic mice subjected to T cell depletion ( without DC depletion ) substantially enhanced HPE and improved immune competence . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Here , we show that IL 16 added to a basal cocktail comprised of granulocyte macrophage colony stimulating factor ( GM CSF ) , IL 4 , Flt 3 ligand ( Flt3L ) , and tumor necrosis factor alpha ( TNF alpha ) does induce the CD 34 ( + ) hematopoietic cells to proliferate in vitro and to differentiate into phenotypically and functionally mature DCs . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
While hematopoietic development was diminished without serum , here we demonstrate with the stromal cell coculture model that addition of the growth factors stem cell factor ( SCF ) , thrombopoietin ( TPO ) , and Flt 3 ligand ( Flt3L ) to serum free media does allow isolation of hematopoietic progenitors . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Expanding the numbers of DC in vivo using the cytokine flt 3 ligand ( flt3L ) enhances the susceptibility of mice to the induction of oral tolerance . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Natural IFN producing and DC progenitors and their respective downstream cell populations express the flt 3 receptor , and Flt 3 ligand ( Flt3L ) ( / ) mice have reduced while Flt3L injected mice develop markedly increased numbers of both cell types . ^^^ In the present study , we show that SU 11657 , a small multitargeted receptor tyrosine kinase inhibitor with Flt 3 affinity , suppressed in vitro natural IFN producing and DC development in Flt3L supplemented mouse whole bone marrow cell cultures in a dose dependant manner , while DC development in GM CSF supplemented cultures was not affected . ^^^ Conversely , Flt3L plasma levels increased massively in inhibitor treated animals , likely via a regulatory feedback loop , without being able to compensate for pharmacological Flt 3 inhibition . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand ( Flt3L ) is a nonredundant cytokine in type 1 interferon producing cell ( IPC ) and dendritic cell ( DC ) development , and IPC and DC differentiation potential is confined to Flt3+ hematopoietic progenitor cells . ^^^ Based on these data , we propose a demand regulated , cytokine driven DC and IPC regeneration model , in which high Flt3L levels initiate a self sustaining , Flt 3 STAT3 and Flt 3 PU . 1 mediated IPC and DC differentiation program in Flt3+ hematopoietic progenitor cells . . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
We have found that in vivo , overexpression of the hematopoietic cytokine Flt 3 ligand ( Flt3L ) expands NKDC in various organs from 2 18 fold . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
CONCLUSIONS AND CLINICAL RELEVANCE : These data suggest that dysregulation of Kit / SCF , Flt3 / Flt3L , and Met / HGF signaling pathways is unlikely to occur in histiocytic sarcomas of dogs and that inhibitors of the Kit , Flt 3 , and Met pathways are unlikely to provide clinical benefit to dogs with histiocytic sarcomas . . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Plasma / serum levels of flt 3 ligand are low in normal individuals and highly elevated in patients with Fanconi anemia and acquired aplastic anemia . ^^^ The flt 3 ligand is a growth factor that stimulates the proliferation of hematopoietic progenitor and stem cells . ^^^ We established a sensitive enzyme linked immunosorbent assay ( ELISA ) to measure the concentration of flt 3 ligand in plasma or serum from normal individuals , as well as in patients with hematopoietic disorders . ^^^ Concentrations of flt 3 ligand in plasma or serum from normal individuals were quite low : only 12 % ( 7 of 60 ) of normal individuals had flt 3 ligand levels above 100 pg / mL ( the limit of detection ) . ^^^ Concentrations of flt 3 ligand in blood are , therefore , specifically elevated to a level that may be physiologically relevant in hematopoietic disorders with a suspected stem cell component . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Effects of human FLT 3 ligand on myeloid leukemia cell growth : heterogeneity in response and synergy with other hematopoietic growth factors . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Specifically , TNF alpha is a potent inhibitor of the clonal growth of primitive and committed murine and human bone marrow progenitors in combination with multiple cytokines , including granulocyte colony stimulating factor ( G CSF ) , CSF 1 , erythropoietin ( Epo ) , stem cell factor ( SCF ) , and flt 3 ligand ( FL ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The flt 3 ligand : a hematopoietic stem cell factor whose activities are distinct from steel factor . ^^^ Among this group are Steel factor ( also known as mast cell growth factor , stem cell factor and kit ligand ) , and the more recently described flt 3 ligand . ^^^ Like Steel factor , the flt 3 ligand has little biological activity on its own , but synergizes well with a number of other colony stimulating factors and interleukins . ^^^ Steel factor stimulates both the proliferation and activation of mast cells , while preliminary data with the flt 3 ligand suggests that it has no effect on mast cells . ^^^ Although the flt 3 ligand and Steel factor each act on early hematopoietic cells , differences in their activities suggest that they are not redundant and are both required for normal hematopoiesis . . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The FLT 3 ligand potently and directly stimulates the growth and expansion of primitive murine bone marrow progenitor cells in vitro : synergistic interactions with interleukin ( IL ) 11 , IL 12 , and other hematopoietic growth factors . ^^^ The recently cloned murine flt 3 ligand ( FL ) was studied for its ability to stimulate the growth of primitive ( Lin Sca 1+ ) and more committed ( Lin Sca 1 ) murine bone marrow progenitor cells , alone and in combination with other hematopoietic growth factors ( HGFs ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Multi level effects of flt 3 ligand on human hematopoiesis : expansion of putative stem cells and proliferation of granulomonocytic progenitors / monocytic precursors . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand stimulates / costimulates the growth of myeloid stem / progenitor cells . ^^^ The present studies evaluated effects of recombinant human ( rhu ) and murine ( rmu ) flt 3 ligand ( flt 3 L ) on colony formation by subsets of myeloid stem and progenitor cells present in low density ( LD ) and cell sorted CD 34 hu cord blood ( CB ) and bone marrow ( BM ) cells and unseparated mu BM cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Surface expression of flt 3 receptor and flt 3 ligand were detected by flow cytometry using biotinylated human flt 3 ligand or biotinylated soluble human flt 3 receptor Fc fusion protein ( flt3R Fc ) , respectively . ^^^ Staining for surface expression of the flt 3 ligand revealed that seven of nine murine cell lines and nine of 15 human cell lines screened were positive by flow cytometry . ^^^ All murine and human cell lines assayed were positive for flt 3 ligand RNA expression by Northern blot analysis , but not all cell lines expressing flt 3 ligand mRNA had detectable surface expression . ^^^ Cells expressing the flt 3 ligand were of the myeloid , B cell and T cell lineages at various stages of differentiation . ^^^ We found that LIF stimulated M 1 cells down regulated surface expression and mRNA levels of the flt 3 receptor , but up regulated expression of the flt 3 ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Expression of FLT 3 receptor and FLT 3 ligand in human leukemia lymphoma cell lines . ^^^ These studies served to illustrate further the importance of the FL FLT 3 ligand receptor system in the regulation of hematopoietic cells . . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The flt 3 ligand supports proliferation of lymphohematopoietic progenitors and early B lymphoid progenitors . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Identification of soluble and membrane bound isoforms of the murine flt 3 ligand generated by alternative splicing of mRNAs . ^^^ We have recently described a novel hematopoietic growth factor , referred to as the flt 3 ligand , that stimulates the proliferation of sub populations of hematopoietic cells that are enriched for stem and progenitor cells . ^^^ We have isolated additional flt 3 ligand isoforms by PCR that contain an extra exon and encode what are predicted to be either a soluble form of the ligand or a longer version of the transmembrane protein . ^^^ We have also isolated cDNAs from murine T cell libraries that encode an isoform of the flt 3 ligand that has an unusual C terminus . ^^^ Genetic mapping studies localize the gene encoding the flt 3 ligand to the proximal portion of mouse chromosome 7 and to human chromosome 19q13 . 3 . . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The recent cloning of the FLT 3 ligand suggesting its important role in the differentiation and proliferation of the hematopoietic stem cells , has confirmed the initial expression analysis showing restricted pattern of receptor expression within the primitive hematopoietic population . ^^^ The construction of a chimeric receptor called FF 3 , between the extracellular region of the CSF 1 receptor fused with the transmembrane and the cytoplasmic regions of FLT 3 , has allowed an analysis in the absence of FLT 3 ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Cloning of the human homologue of the murine flt 3 ligand : a growth factor for early hematopoietic progenitor cells . ^^^ Using a fragment of the murine flt 3 ligand as a probe , we have succeeded in cloning a human flt 3 ligand from a human T cell lambda gt 10 cDNA library . ^^^ A recombinant soluble form of the human flt 3 ligand stimulates the proliferation and colony formation of a subpopulation of human bone marrow cells that are CD34+ and are enriched for primitive hematopoietic cells . ^^^ In addition , the human flt 3 ligand also stimulates the proliferation of cells expressing murine flt 3 receptors . ^^^ Northern blot analysis shows widespread expression of flt 3 ligand mRNA transcripts in human tissues . . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The effect of human flt 3 ligand on committed progenitor cell production from normal , aplastic anaemia and Diamond Blackfan anaemia bone marrow . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Stimulation of patient samples with FLT 3 ligand resulted in autophosphorylation of the FLT 3 receptor , suggesting the receptor is functional in these cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The FLT 3 ligand is a direct and potent stimulator of the growth of primitive and committed human CD34+ bone marrow progenitor cells in vitro . ^^^ The present studies investigated the effects of the recently cloned flt 3 ligand ( FL ) on the in vitro growth and differentiation of primitive and committed subsets of human CD34+ bone marrow ( BM ) progenitor cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand induces proliferation of quiescent human bone marrow CD34+CD38 cells and maintains progenitor cells in vitro . ^^^ In previous studies using stroma free short term assays , Flt 3 ligand ( FL ) has been shown to induce proliferation of HPC at proportions similar to or less than c kit ligand ( steel factor , SF ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The novel hematopoietic growth factor FLT 3 ligand ( FL ) is the cognate ligand for the FLT 3 , tyrosine kinase receptor ( R ) , also referred to as FLK 2 and STK 1 . ^^^ Expression of FLT 3 receptor and response to FLT 3 ligand by leukemic cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Accelerated cell cycling of hematopoietic progenitors by the flt 3 ligand that is modulated by transforming growth factor beta . ^^^ We examined the effects of the flt 3 ligand ( FL ) on cell cycling of hematopoietic progenitors in serum free culture . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Stem cell factor , but not flt 3 ligand , induces differentiation and activation of human mast cells . ^^^ We also explored the effect of flt 3 ligand ( FL ) on the human mast cell line HMC 1 and mRNA expression of flt3 / flk2 on in vitro developed human mast cells and HMC 1 . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Other growth factors tested ( ie GM CSF , G CSF , IL 3 , FLT 3 ligand and EPO ) were much less active or stimulated phosphorylation of other proteins . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Functional analysis of five mAbs ( SF 1 series ) revealed that all of them can mimic to variable extents the activity of the FLT 3 ligand ( FL ) upon receptor activation and modulation , while only one mAb weakly inhibited ligand binding . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Effects of FLT 3 ligand on human leukemia cells . 2 . ^^^ We have previously shown that the growth factor FLT 3 ligand ( FL ) is mitogenic for human primary and continuously cultured myeloid leukemia cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The effects of human FLT 3 ligand on in vitro human megakaryocytopoiesis . ^^^ Using a plasma clot clonal assay and a long term bone marrow culture ( LTBMC ) system , we studied the effects of the recently cloned human flt 3 ligand ( FL ) alone and in combination with granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 3 ( IL 3 ) , or stem cell factor ( c kit ligand [ KL ] ) on human megakaryocytopoiesis . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Effects of Flt 3 ligand and interleukin 7 on in vitro growth of acute lymphoblastic leukemia cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Incubation of the same CD34+ CD 38 starting populations as single cells in a defined ( serum free ) liquid medium supplemented with Flt 3 ligand , steel factor , IL 3 , IL 6 , granulocyte colony stimulating factor , and nerve growth factor resulted in the proliferation of initial cells to produce clones of from 4 to 1000 cells within 10 days , approximately 40 % of which included > or = 1 LTC IC . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Incubation with single recombinant cytokines from a broad spectrum of growth factors established that the strongest proliferation response of MUTZ 2 cells was elicited by FLT 3 ligand , granulocyte colony stimulating factor ( G CSF ) , macrophage CSF ( M CSF ) , interferon gamma ( IFN gamma ) and stem cell factor ( SCF ) , whereas granulocyte macrophage CSF ( GM CSF ) , M CSF , interleukin 3 ( IL 3 ) and SCF were the most effective growth factors in inducing proliferation of MUTZ 3 . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Ability of flt 3 ligand to stimulate the in vitro growth of primitive murine hematopoietic progenitors is potently and directly inhibited by transforming growth factor beta and tumor necrosis factor alpha . ^^^ The recently cloned flt 3 ligand ( FL ) stimulates the growth of primitive hematopoietic progenitor cells through synergistic interactions with multiple other cytokines . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The FLT 3 ligand , FL , is a new cytokine which acts on hematopoietic progenitors in synergy with other cytokines . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In the present study , the flt 3 ligand ( FL ) , which , in combination with other cytokines , has overlapping activities with SCF on myeloid cell production from uncommitted progenitors , was investigated for its ability to induce selective stroma independent B cell commitment from uncommitted Lin Sca 1+ bone marrow progenitor cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Regulation of colony forming cell generation by flt 3 ligand . ^^^ Flt 3 ligand ( flt 3l ) had little or no effect when added alone to cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Murine flt 3 ligand protects M 1 leukemic cells from LIF induced differentiation and suppression of self renewal . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand prolongs survival of CD34++ + human umbilical cord blood myeloid progenitors in serum depleted culture medium . ^^^ Flt 3 Ligand ( L ) , a newly identified stimulating / co stimulating cytokine for hematopoietic stem and progenitor cells , was assessed for its capacity , in comparison to that of steel factor , to prolong the survival in culture of hematopoietic stem and progenitor cells from CD34++ + human cord blood . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand supports the differentiation of early B cell progenitors in the presence of interleukin 11 and interleukin 7 . ^^^ Flt 3 ligand ( FL ) is a growth factor which stimulates the proliferation of stem cells and early progenitors . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Prolongation of the second phase of the assay ( when the cells are maintained under lymphoid LTC conditions ) from 7 to 10 days and inclusion of Flt 3 ligand ( FL ) in the assay used to detect the production of B lineage cells ( CFU pre B ) increased the average yield of CFU pre B per LTC ICML by a factor of 3 to 20 fold ; however , neither of these manipulations increased the frequency of LTC ICML detected . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Advances include the discoveries of the growth factors thrombopoietin and Flt 3 ligand , the development of a variety of bioreactor systems , and results from preliminary clinical trials that demonstrate the efficacy of ex vivo expanded hematopoietic cells for transplantation therapy . . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The flt 3 ligand promotes the survival of primitive hemopoietic progenitor cells with myeloid as well as B lymphoid potential . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Hematologic effects of flt 3 ligand in vivo in mice . ^^^ We have investigated the effects of in vivo treatment with flt 3 ligand ( FL ) on murine hematopoiesis , including mobilization of progenitors into the peripheral blood ( PB ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
All cases were also tested for their proliferative response to human FLT 3 ligand ( FL ) and KIT ligand ( KL ) and for colony formation in the presence of single or associated cytokines . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Finally , the effect of IL 3 , IL 6 , SCF , and Flt 3 ligand ( FL ) on cycle induction was examined . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Biochemical and genetic characterization of multiple splice variants of the Flt 3 ligand . ^^^ We have performed a comprehensive analysis of cell lines and tissues to compare and contrast the expression patterns of Flt 3 ligand ( FL ) , c Kit ligand ( KL ) , and macrophage colony stimulating factor as well as their receptors , Flt 3 , c Kit , and c Fms . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
FLT 3 ligand is a hematopoietic growth factor that plays a key role in growth of primitive hematopoietic cells . ^^^ Using human 125I FLT 3 ligand , we have identified and characterized surface FLT 3 receptors on normal and malignant human hematopoietic cells and cell lines . ^^^ Erythroleukemic and megakaryocytic leukemia cell lines ( n = 5 ) bound little to no 125I FLT 3 ligand . ^^^ Scatchard analysis of 125I FLT 3 ligand binding data shows that three myeloid leukemia cell lines , ML 1 , AML 193 , and HL 60 , as well as normal human marrow mononuclear cells , exhibit high affinity FLT 3 receptors . ^^^ Crosslinking of 125I FLT 3 ligand to FLT 3 receptors on the surface of ML 1 myeloid leukemia cells indicates that the FLT 3 ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In quiescent HPCs the receptors for early acting [ flt 3 ligand ( FL ) , c kit ligand ( KL ) , bFGF , interleukin 6 ( IL 6 ) ] and multilineage [ IL 3 , granulocyte macrophage CSF ( GM CSF ) ] HGFs are expressed at significant levels but with different patterns , eg , kit and flt 3 are detected on a majority and minority of HPCs , respectively , whereas IL 3Rs and GM CSFRs are present on almost all HPCs . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand stimulates proliferation and inhibits apoptosis of acute myeloid leukemia cells : regulation of Bcl 2 and Bax . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
This review will focus on recently characterized growth factors including c kit ligand / stem cell factor , flt 3 ligand , c mpl ligand / thrombopoietin and interleukins 11 , 4 , 7 , 10 , 12 and 13 . . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In this study , the effects of c kit ligand ( KL ) and flt 3 ligand ( FL ) were compared in the presence of IL 3 , GM CSF , and erythropoietin ( 3 / GM / EPO ) , using frequent medium exchange cultures of human bone marrow mononuclear cells ( BMMNC ) and CD 34 enriched cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
New inducers and inhibitors of apoptosis have been reported , particularly the role of stromal environment , thrombopoietin , erythropoetin and flt 3 ligand has been mentioned . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Retroviral mediated gene transfer of Flt 3 ligand enhances proliferation and MAP kinase activity of AML 5 cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Addition of Flt 3 ligand to long term cultures resulted in a 2 3 fold greater increase in the number of CD19+ cells generated after 4 8 weeks . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
FLT 3 ligand preserves the ability of human CD34+ progenitors to sustain long term hematopoiesis in immune deficient mice after ex vivo retroviral mediated transduction . ^^^ We hypothesized that the cytokine FLT 3 ligand ( FL ) might be able to replace the maintenance role provided by the stroma . ^^^ LN provirus was detected by polymerase chain reaction in the marrow recovered from 9 of 10 mice transplanted with human CD34+ cells transduced with stromal support , 5 of 11 mice that received human cells transduced in suspension culture with FL , but none of the 10 mice that received human cells transduced in suspension culture without FL We conclude that FLT 3 ligand , in conjunction with IL 3 , IL 6 , and SCF , preserves the generative capacity of primitive human hematopoietic cells during in vitro transductions in suspension culture . . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Coexpression of flt 3 ligand / flt 3 and SCF / c kit signal transduction system in bile duct ligated SI and W mice . ^^^ It has been reported that the flt 3 ligand ( FL ) / flt 3 system can synergize with the SCF / c kit system and stimulate the proliferation of hematopoietic cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
MGDF also enhanced interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , c kit ligand ( KL ) and FLT 3 ligand ( FL ) stimulated blast cell proliferation . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Thrombopoietin augments ex vivo expansion of human cord blood derived hematopoietic progenitors in combination with stem cell factor and flt 3 ligand . ^^^ We studied the effects of stem cell factor ( SCF ) and flt 3 ligand ( FL ) on the ex vivo expansion of human umbilical cord blood ( CB ) derived CD34+ cells in combination with various cytokines , including interleukin ( IL ) 3 , IL 6 , IL 11 , and c Mpl ligand ( thrombopoietin , TPO ) , in a short term serum free liquid suspension culture system . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Differential effects of Flk 2 / Flt 3 ligand and stem cell factor on murine thymic progenitor cells . ^^^ While Flt 3 ligand ( Flt 3L ) has been shown to be involved in early bone marrow hemopoiesis , its role in thymopoiesis has not been thoroughly examined . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Previous studies have shown that primitive human hematopoietic cells detectable as long term culture initiating cells ( LTC ICs ) and colony forming cells ( CFCs ) can be amplified when CD 34 ( + ) CD 38 ( ) marrow cells are cultured for 10 days in serum free medium containing flt 3 ligand ( FL ) , Steel factor ( SF ) , interleukin ( IL ) 3 , IL 6 , and granulocyte colony stimulating factor . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Synergistic effect of FLT 3 ligand on the granulocyte colony stimulating factor induced mobilization of hematopoietic stem cells and progenitor cells into blood in mice . ^^^ We have previously shown that FLT 3 ligand ( FL ) mobilizes murine hematopoietic primitive and committed progenitor cells into blood dose dependently . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Striking augmentation of hematopoietic cell chimerism in noncytoablated allogeneic bone marrow recipients by FLT 3 ligand and tacrolimus . ^^^ The influence of granulocyte macrophage colony stimulating factor ( GM CSF ) and the recently identified hematopoietic stem progenitor cell mobilizing factor flt 3 ligand ( FL ) on donor leukocyte microchimerism in noncytodepleted recipients of allogeneic bone marrow ( BM ) was compared . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Tpo alone stimulated limited clonal growth , but synergized with c kit ligand ( KL ) , flt 3 ligand ( FL ) , or IL 3 to potently enhance clonogenic growth . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand synergizes with granulocyte colony stimulating factor to increase neutrophil numbers and to mobilize peripheral blood stem cells with long term repopulating potential . ^^^ Flt 3 ligand ( FL ) shares many features with stem cell factor ( SCF ) , a widely documented cofactor for peripheral blood progenitor cell ( PBPC ) mobilization . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand induces tumor regression and antitumor immune responses in vivo . ^^^ Daily treatment of mice with recombinant human Flt 3 ligand ( huFlt3L ) results in a dramatic numerical increase in the number of dendritic cells ( DCs ) in vivo . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Human FLT 3 ligand acts on myeloid as well as multipotential progenitors derived from purified CD34+ blood progenitors expressing different levels of c kit protein . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Chronic expression of murine flt 3 ligand in mice results in increased circulating white blood cell levels and abnormal cellular infiltrates associated with splenic fibrosis . ^^^ The effect of chronic expression of flt 3 ligand ( FL ) on in vivo hematopoiesis was studied . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
However , NK cells were derived from CD34+ CD 38 and CD34+ CD38+ fetal liver cells cultured in the presence of IL 15 , IL 7 , and Flt 3 ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
To expand on the functional properties of Flt 3 ligand ( FL ) in vivo we treated nonhuman primates with FL and tested its ability to mobilize stem / progenitor cells when given alone or in combination with granulocyte colony stimulating factor ( G CSF ) treatment . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Administration of the early acting cytokine flt 3 ligand to these strains resulted in activation of quiescent primitive stem cells exclusively in strains with high endogenous stem cell numbers ( DBA and AKR ) , but was unrelated to strain specific progenitor cell cycling . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
FLT 3 ligand induces the generation of functionally active dendritic cells in mice . ^^^ FLT 3 ligand ( FL ) is a recently described hematopoietic growth factor that stimulates the proliferation and differentiation of hematopoietic progenitors . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Using a recently described serum free culture system of purified human CD34+ progenitor cells , we show here a critical cooperation of flt 3 ligand ( FL ) with transforming growth factor beta 1 ( TGF beta 1 ) in the induction of in vitro dendritic cell / Langerhans cell ( DC / LC ) development . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Incubation of purified CD 34 ( + ) CD 38 ( ) human CB cells in serum free medium containing flt 3 ligand , Steel factor , interleukin 3 , interleukin 6 , and granulocyte colony stimulating factor for 5 8 days resulted in a 100 fold expansion of colony forming cells , a 4 fold expansion of LTC IC , and a 2 fold ( but significant , P < 0 . 02 ) increase in CRU . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Developmental pathways of dendritic cells in vivo : distinct function , phenotype , and localization of dendritic cell subsets in FLT 3 ligand treated mice . ^^^ We have recently shown that Flt 3 ligand administration dramatically increases dendritic cell ( DC ) numbers in various mouse tissues . ^^^ These have been designated : population C ( CD11c ( bright ) CD11b ( bright ) ) , D ( CD11c ( bright ) CD11b ( dull ) ) , and E ( CD11c ( bright ) CD11b ( negative ) ) This report demonstrates that the mature DC subsets ( C , D , and E ) from Flt 3 ligand treated mice differ with respect to phenotype , geographic localization , and function . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
New molecule under study : Flt 3 ligand may mobilize dendritic cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The granulocyte colony stimulating factor receptor is required for the mobilization of murine hematopoietic progenitors into peripheral blood by cyclophosphamide or interleukin 8 but not flt 3 ligand . ^^^ In contrast , mobilization of HPC in response to flt 3 ligand was nearly normal . ^^^ These results show that the G CSFR is required for mobilization in response to cyclophosphamide or IL 8 but not flt 3 ligand and suggest that the G CSFR may play an important and previously unexpected role in HPC migration . . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Analysis of the mitogenic activity of interleukin 3 ( IL 3 ) , Steel factor ( SF ) , and flt 3 ligand ( FL ) on acute myelogenous leukemia ( AML ) blasts using the short term endpoints of proliferation in 3H thymidine ( 3H Tdr ) incorporation assays or methylcellulose cultures ( colony assays ) showed that greater than 90 % of samples contained cells that were responsive to one or more of these cytokines . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Low proliferative capacity was observed after 1 week in culture in the presence of stem cell factor ( SCF ) + Flt 3 ligand ( FL ) + G CSF . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand synergizes with granulocyte macrophage colony stimulating factor or granulocyte colony stimulating factor to mobilize hematopoietic progenitor cells into the peripheral blood of mice . ^^^ We investigated the capacity of several different growth factors , including human flt 3 ligand ( FL ) , alone and in combination with granulocyte macrophage colony stimulating factor ( GM CSF ) or granulocyte colony stimulating factor ( G CSF ) , to mobilize colony forming cells ( CFU ) into the peripheral blood ( PB ) of mice . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Culture of lineage marker ( Lin ) , c kit+ , Sca2+ , interleukin ( IL ) 2 / 15Rbeta ( CD 122 ) marrow cells in IL 6 , IL 7 , stem cell factor ( SCF ) , and flt 3 ligand ( flt 3 L ) for 5 6 d followed by IL 15 alone for an additional 4 5 d expanded the starting population 30 40 fold and gave rise to a virtually pure population of NK1 . 1+ , CD 3 cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand enhances the yield of primitive cells after Ex vivo cultivation of CD34+ CD38dim cells and CD34+ CD38dim CD33dim HLA DR+ cells . ^^^ Flt 3 ligand ( FL ) has been proposed as a possible modulator of early hematopoietic cell growth . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Biology and potential clinical applications of flt 3 ligand . ^^^ The flt 3 ligand is a member of a small family of growth factors that stimulate the proliferation of hematopoietic cells . ^^^ Both flt 3 ligand and Steel factor stimulate the proliferation of early progenitor or stem cells . ^^^ One major difference between the two factors appears to be their effect on mast cells , which Steel factor stimulates , but flt 3 ligand does not . ^^^ Although flt 3 ligand and Steel factor each act on early hematopoietic cells , differences in their activities suggest that they are not redundant and are both required for normal hematopoiesis . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Finally , reports are reviewed of two new factors that stimulate the growth of acute myeloblastic leukemia cells ( thrombopoietin and Flt 3 ligand ) and a factor that inhibits the autocrine growth characteristics of acute myeloblastic leukemia cells ( interleukin 10 ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Bidirectional effect of interleukin 10 on early murine B cell development : stimulation of flt 3 ligand plus interleukin 7 dependent generation of CD 19 ( ) ProB cells from uncommitted bone marrow progenitor cells and growth inhibition of CD 19 ( + ) ProB cells . ^^^ We recently showed that the flt 3 ligand ( FL ) has a unique ability to interact with interleukin 7 ( IL 7 ) to directly and selectively promote B cell development from murine bone marrow progenitor cells with a combined myeloid and lymphoid potential . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In vivo administration of flt 3 ligand markedly stimulates generation of dendritic cell progenitors from mouse liver . ^^^ We examined the ex vivo growth , phenotype , and function of these cells after the administration to mice of the recently cloned hemopoietic growth factor flt 3 ligand ( FL ) , which is highly effective in mobilizing stem / progenitor cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In vivo effects of flt 3 ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
When the CD34+ / c kit < low cells were cultured with cytokines ( flt 3 ligand , interleukin 6 and interleukin 7 ) plus immobilized anti CD 34 monoclonal antibody ( to crosslink CD 34 molecules ) , c kit molecules were clearly induced within 24 h . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In an effort to obtain human B lymphoid , as well as T lymphoid and myeloid cell development in the mice , CD34+ cells were coinjected with human marrow stromal cells engineered to secrete human IL 2 , IL 7 , stem cell factor or FLT 3 ligand , + / IL 3 . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Induction of dendritic cells ( DC ) by Flt 3 Ligand ( FL ) promotes the generation of tumor specific immune responses in vivo . ^^^ Daily treatment of mice with human Flt 3 ligand ( FL ) induces the production of large numbers of MHC class II+ , CD11c+ , DEC205+ dendritic cells ( DC ) in both lymphoid and nonlymphoid tissues . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand was required during the 7 day ex vivo culture to maintain the ability of the cells to sustain long term engraftment and hematopoiesis in the mice . . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand : stem / progenitor cell factors with overlapping yet distinct activities . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
FLT 3 ligand administration inhibits tumor growth in murine melanoma and lymphoma . ^^^ Administration of FLT 3 ligand ( FL ) results in a reversible accumulation of functionally active DCs in both lymphoid and nonlymphoid tissues . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
FLT 3 ligand and marrow stroma derived factors promote CD3gamma , CD3delta , CD3zeta , and RAG 2 gene expression in primary human CD34+LIN DR marrow progenitors . ^^^ This population was then used to evaluate the effects of supplemental cytokines ( interleukin 2 [ IL 2 ] , IL 3 , IL 7 , c kit ligand ) , FLT 3 ligand ( FL ) , and stroma derived factors on lymphoid differentiation in vitro . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Cell numbers in the suspension cultures initiated with Flt3high cells were maintained in the presence of Flt 3 ligand ( FL ) alone , and increased in response to FL plus kit ligand ( KL ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
FLT 3 ligand mobilizes hematopoietic primitive and committed progenitor cells into blood in mice . ^^^ We investigated the effects of the administration of FLT 3 ligand ( FL ) on mobilization of primitive and committed progenitor cells in mice . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Other cytokines tested ( ie GM CSF , G CSF , IL 3 , FLT 3 ligand , TPO , EPO ) were much less active or stimulated phosphorylation of other proteins . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The potential role of FLT 3 ligand in progenitor and primitive hematopoietic cell expansion . ^^^ Interestingly , the addition of 100 ng / ml of Flt 3 ligand ( FL ) significantly enhanced the amplification of total cells ( 276 fold ) , CFU GM ( 54 fold ) and BFU E ( 15 fold ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Distinct actions of Flt 3 ligand and stem cell factor on myeloid lineage selection and maturation of granulocytes versus macrophages . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Augmentation of dendritic cells in murine organ donors by Flt 3 ligand alters the balance between transplant tolerance and immunity . ^^^ Treatment of mice with the recently cloned hemopoietic growth factor Flt 3 ligand ( FL ; 10 microg / day for 10 days ) resulted in a large increase in myeloid lineage cells within the liver . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand increases microchimerism but can prevent the therapeutic effect of donor bone marrow in transiently immunosuppressed cardiac allograft recipients . ^^^ C3H ( H2k ) mice received 50 10 10 ( 6 ) B 10 ( H2b ) bone marrow ( BM ) cells either alone or with flt 3 ligand ( FL ) ( 10 microg / day ) , tacrolimus ( 2 mg / kg / day ) , or both agents for 7 days . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The sI DC population is expanded 100 fold after an in vivo flt 3 ligand treatment . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The recently identified cytokine FLT 3 ligand further dramatically enhanced in vitro LC development and even allowed efficient in vitro generation of LC colonies from serum free single cell cultures of CD34+ hemopoietic progenitor cells . . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
CB was cryopreserved using standard methods and the thawed unpurified cells were used to initiate small scale cultures supplemented with PIXY 321 , flt 3 ligand , and erythropoietin in serum containing medium . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The number of primitive human hematopoietic long term culture initiating cell derived colonies ( LTC IC derived colonies ) generated from CD34+ and CD34+ / HLA DRlo peripheral blood progenitor `` stem ' ' cells ( PBSCs ) increased during 7 days of treatment in this vector containing medium in the presence of IL 3 , SCF , and flt 3 ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The addition of Flt 3 ligand ( FL ) increases CD41+ cell expansion to approximately 380 fold over input levels . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand , MGDF , Epo and G CSF enhance ex vivo expansion of hematopoietic cell compartments in the presence of SCF , IL 3 and IL 6 . ^^^ The aim of the study is to define the ability of Flt 3 ligand , MGDF , Epo and G CSF to modulate the expansion of different hematopoietic compartments in association with a basic cocktail of SCF + IL 3 + IL 6 ( S 36 ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Cells with a dendritic morphology and expressing HLA DR , CD1a , S 100 and CD 83 were maximally expanded under serum free conditions with the addition of SCF , GM CSF , TNF alpha , TGF beta and Flt 3 ligand ( fold increase of CD1a+ cells = 102 + / 32 after 2 weeks of culture ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
However , after addition of FLT 3 ligand , the frequency of primitive progenitors able to develop along the NK lineage increased 10 fold . ^^^ Addition of FLT 3 ligand to IL 2 , IL 7 SCF , and stromal factors are important in early stages of NK development . . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
We analyzed the effect of tumor necrosis factor ( TNF ) alpha on the differentiation and viability of dendritic cells ( DC ) generated from cord blood CD34+ progenitors cultured for five days with GM CSF , Flt 3 ligand ( FL ) , and stem cell factor ( SCF ) , and then with GM CSF only [ TNF ( ) cultures ] . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
However , few studies detail the effects of hematopoietic cytokines such as IL 3 , Flt 3 ligand , G CSF , Tpo , and Epo or correlate the effects on virus replication . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
RT PCR analysis of cytokine and growth factor mRNA in MSCs and MDSCs revealed a very similar pattern of mRNAs including IL 6 , 7 , 8 , 11 , 12 , 14 , and 15 , M CSF , Flt 3 ligand , and SCF . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
This demonstrated that leukemia cell populations usually contain transcripts for IL1beta , TNF alpha , flt 3 and flt 3 ligand in vivo and that transcripts for the other cytokines only appear after the leukemia cells are processed in vitro . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Haematopoietic action of flt 3 ligand on cord blood derived CD 34 positive cells expressing different levels of flt 3 or c kit tyrosine kinase receptor : comparison with stem cell factor . ^^^ We compared the effect of human flt 3 ligand ( FL ) and stem cell factor ( SCF ) on cord blood ( CB ) derived CD34+ cells expressing different levels of flt 3 or c kit tyrosine kinase ( TK ) receptor in clonal cell culture . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Biologic effects and potential clinical applications of Flt 3 ligand . ^^^ Flt 3 ligand ( FL ) is a member of a small family of growth factors that stimulate the proliferation of hematopoietic cells by binding to and activating distinct tyrosine kinase receptors . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Newer growth factors such as stem cell factor , thrombopoietin , Flt 3 ligand , and interleukins have shown promising results in the laboratory , and some have been used in clinical trials . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Donor pretreatment with Flt 3 ligand augments antidonor cytotoxic T lymphocyte , natural killer , and lymphokine activated killer cell activities within liver allografts and alters the pattern of intragraft apoptotic activity . ^^^ Flt 3 ligand ( FL ) is a recently cloned hematopoietic cytokine that strikingly augments functional dendritic cells ( DCs ) within lymphoid and nonlymphoid tissue . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
However , ex vivo expansion of the sorted subsets with interleukin 3 , stem cell factor and FLT 3 ligand for 2 weeks resulted in a significant production of O 2 and H2O2 / HOCl by CD34+ / CD117low cell fraction , compared to the same sorted but not expanded counterparts . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Time course studies revealed that the combination of Flt 3 ligand ( FL ) , Steel factor ( SF ) and interleukin 3 ( IL 3 ) did not elicit as large an amplification of the long term culture initiating cell ( LTC IC ) population in serum free cultures of CD34+ CD 38 cord blood ( CB ) cells as was obtained in similar cultures of adult human CD34+ CD 38 bone marrow ( BM ) cells ( 4 5 90 fold maximum increases ) , even though both total and colony forming cell ( CFC ) numbers initially increased more rapidly in CB cultures . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The BCP cell lines display surface receptors for interferon gamma ( CD 119 ) , interleukin 7 ( CD 127 ) and FLT 3 ligand ( CD 135 ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
FLT 3 ligand provides hematopoietic protection from total body irradiation in rabbits . ^^^ We tested the radioprotective potential of FLT 3 ligand , an early acting hematopoietic cytokine , alone and in combination with a late acting cytokine , granulocyte colony stimulating factor ( G CSF ) . ^^^ Recombinant human ( rh ) FLT 3 ligand at a dose of 500 microg / kg and / or rhG CSF at a dose of 10 microg / kg were administered for 14 days subcutaneously daily , beginning either 2 days before or the day after TBI . ^^^ In contrast , 11 of 12 animals given FLT 3 ligand , with or without G CSF , survived . ^^^ Radioprotection was best in the group given FLT 3 ligand together with G CSF before TBI . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Interleukin 3 and Flt 3 ligand induce adhesion of Baf3 / Flt3 precursor B lymphoid cells to fibronectin via activation of VLA 4 and VLA 5 . ^^^ However , little is known regarding the potential influence of IL 3 on this process for precursor B cells and Flt 3 ligand has not yet been implicated in induction of adhesion of any blood cell types to extracellular matrix components . ^^^ IL 3 induced adhesion of Baf 3 and Baf3 / Flt3 cells to FN , while Flt 3 ligand induced adhesion of Baf3 / Flt3 cells only . ^^^ Whereas both Baf 3 and Baf3 / Flt3 cells expressed VLA 4 and 5 integrins as FN receptors , expression levels of VLA 4 and 5 were not affected by IL 3 or Flt 3 ligand treatment . ^^^ However , blocking experiments using anti integrin antibodies showed that cytokine induced adhesion of cells depended on both VLA 4 and 5 suggesting that IL 3 and Flt 3 ligand activated these integrins . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Even when flt 3 ligand ( 100 ng / ml ) was added to cultures , only two of five animals ( 40 % ) engrafted ( p = NS versus studies without flt 3 ligand ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Of the two hematopoietic growth factors , acting at the early stages of hematopoiesis , circulating levels of flt 3 ligand are highly elevated in patients with aplastic anemia , whereas those of stem cell factor ( SCF ) are essentially normal . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Cord blood CD34+ cells were cultured with granulocyte macrophage colony stimulating factor ( GM CSF ) and tumor necrosis factor alpha ( TNF alpha ) , to which stem cell factor and Flt 3 ligand were added for 5 days . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand ( FL ) is a recently identified cytokine having a central role in the proliferation , survival and differentiation of early murine and human hematopoietic precursor / stem cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Marginally significant further increases in chimerism and / or reductions in chronic heart rejection beyond those achieved with adjunct bone marrow alone were associated with additional treatment with the growth factors Flt 3 ligand , granulocyte colony stimulating factor , and a recombinant molecular variant of interleukin 6 ( interleukin 6 mutein ) but not with hepatocyte growth factor or lisofylline . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Improved gene transfer into baboon marrow repopulating cells using recombinant human fibronectin fragment CH 296 in combination with interleukin 6 , stem cell factor , FLT 3 ligand , and megakaryocyte growth and development factor . ^^^ The highest level of gene transfer was obtained after 24 hours of prestimulation followed by 48 hours of incubation on CH 296 in vector containing medium in the presence of multiple hematopoietic growth factors ( interleukin 6 , stem cell factor , FLT 3 ligand , and megakaryocyte growth and development factor ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Leukemic predisposition of mice transplanted with gene modified hematopoietic precursors expressing flt 3 ligand . flt3 / flk 2 ligand ( FL ) is a cytokine that exhibits synergistic activities in combination with other early acting factors on subpopulations of hematopoietic stem / progenitor cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Treatment of non obese diabetic ( NOD ) / Severe combined immunodeficient mice ( SCID ) with flt 3 ligand and interleukin 7 impairs the B lineage commitment of repopulating cells after transplantation of human hematopoietic cells . ^^^ Many studies , including murine in vitro and in vivo data and human in vitro experiments , have suggested that flt 3 ligand ( FL ) and / or Interleukin 7 ( IL 7 ) promotes T and B cell development . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Expansion of functional NK cells in multiple tissue compartments of mice treated with Flt 3 ligand : implications for anti cancer and anti viral therapy . ^^^ We examined the in vivo effects of recombinant human Flt 3 ligand ( Flt 3 L ) , a recently cloned potent hemopoietic cytokine , on NK cell development in mice . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Circulating CD34+ cell populations characterized by a low rate ( up to five ) or high rate ( more than five ) of cell divisions were isolated from 8 d cultures in the presence of stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , granulocyte colony stimulating factor ( G CSF ) , erythropoietin ( EPO ) , Flt 3 ligand and Peg rHu megakaryocyte growth and development factor ( Peg rHuMGDF ) using the fluorescent dye 5 , 6 carboxyfluorescein diacetate succinimidyl ester ( CFDA SE ) and flow cytometric cell sorting . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
CD34+ cells purified from leukapheresis product were seeded at 1 10 10 ( 4 ) cells / mL in complete medium supplemented with GM CSF , TNF alpha , IL 4 , c kit ligand , and flt 3 ligand ( FL ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Effect of FLT 3 ligand and granulocyte colony stimulating factor on expansion and mobilization of facilitating cells and hematopoietic stem cells in mice : kinetics and repopulating potential . ^^^ Here we investigated the effect of flt 3 ligand ( FL ) and granulocyte colony stimulating factor ( G CSF ) on the mobilization of facilitating cells ( FC ) and HSC into peripheral blood ( PB ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand promotes the generation of a distinct CD 34 ( + ) human natural killer cell progenitor that responds to interleukin 15 . ^^^ Flt 3 ligand ( FL ) is also produced by BM stromal cells and has homology with KL . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
By reverse transcription polymerase chain reaction , the expression by MMHs of cytokines involved in the survival and self renewal of early progenitor cells ( stem cell factor and FLT 3 ligand ) as well as those acting at different stages of progenitor differentiation ( interleukin [ IL ] 1beta , IL 3 , leukemia inhibitory factor , IL 6 , granulocyte macrophage colony stimulating factor , granulocyte colony stimulating factor , macrophage colony stimulating factor , and thrombopoietin ) was shown . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
When combinations of cytokines were used over 7 days , between 5 % ( FLT 3 ligand [ FLT 3L ] , stem cell factor [ SCF ] , IL 3 , IL 6 , granulocyte colony stimulating factor [ G CSF ] , beta nerve growth factor [ betaNGF ] ) and 22 % ( FLT 3L + HGF ) of the cells remained undivided , between 15 % ( HGF , IL 1 , IL 11 , G CSF ) and 68 % ( SCF + TPO ) of the cells had doubled and between 27 % ( FLT 3L + TPO ) and 70 % ( HGF , IL 1 , IL 11 , G CSF ) died . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Prevention of peripheral tolerance by a dendritic cell growth factor : flt 3 ligand as an adjuvant . ^^^ Here we show that administration of Flt 3 ligand ( FL ) , a cytokine capable of inducing large numbers of dendritic cells ( DCs ) in vivo , ( a ) dramatically enhances the sensitivity of antigen specific B and T cell responses to systemic injection of a soluble protein , through a CD 40 CD40 ligand dependent mechanism ; ( b ) influences the class of antibody produced ; and ( c ) enables productive immune responses to otherwise tolerogenic protocols . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
ROSA 26 marrow mononuclear cells ( containing the beta geo transgene that encodes beta galactosidase and neomycin resistance activities ) were cultured in the presence of macrophage colony stimulating factor and flt 3 Ligand for 6 days to generate monocytic cells at all stages of maturation . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The culture system features the use of a serum free medium supplemented with a cocktail of cytokines including flt 3 ligand , interleukin 3 ( IL 3 ) , stem cell factor ( SCF ) , and IL 2 . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Other mouse models have indicated an unexpected role for flt 3 ligand in early NK cell development as well as minor roles for stem cell factor and IL 7 in expanding NK cell progenitor numbers . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
We have used the bnx / hu xenograft system coupled with single colony inverse PCR to determine that human stem cells require stromal support , fibronectin support with cytokines , or the presence of Flt 3 ligand during a 72 h ex vivo culture to maintain the ability to sustain long term multilineage hematopoiesis . . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Progenitors were cultured in limiting dilutions with interleukin 7 ( IL 7 ) , flt 3 ligand ( FL ) , c kit ligand ( KL ) , IL 3 , IL 2 , and AFT 024 , a murine fetal liver line , which supports culture of transplantable murine stem cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
BmDC cultured under various conditions ( granulocyte macrophage CSF ( GM CSF ) or GM CSF plus IL 4 alone or in combination with Flt 3 ligand , TNF alpha , LPS , or CD 40 ligand ( CD40L ) ) were analyzed morphologically , phenotypically , and functionally and were tested for their ability to promote prophylactic and / or therapeutic antitumor immunity . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The latter were derived from fresh C3H or C57BL / 6 liver NPC , or from C3H or C57BL / 6 mice treated for 10 days by i . v . infusion of human Flt 3 ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Synergy of growth factors during mobilization of peripheral blood precursor cells with recombinant human Flt 3 ligand and granulocyte colony stimulating factor in rabbits . ^^^ The potential of recombinant human ( rh ) Flt 3 ligand ( FL ) , alone or in combination with other recombinant growth factors , to mobilize peripheral blood precursor cells ( PBPCs ) was examined in an animal model . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The results show that using a growth factor combination including granulocyte macrophage colony stimulating factor plus tumor necrosis factor alpha plus interleukin 4 plus stem cell factor plus Flt 3 ligand , more than 70 % of DCs derived from CB or MPB CD34+ cells can be transduced . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
NB from four infants was expanded by culturing the blood samples in the presence of thrombopoietin ( Tpo ) , interleukin 1beta ( IL1beta ) , IL 3 , IL 6 , flt 3 ligand and stem cell factor ( SCF ) for 7 d . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Cultures were incubated with human cytokines ( IL 7 , FLT 3 ligand and Steel Factor ) , or remained untreated . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Purified human CD34+ cells were cocultured with PMVECs for 7 days in the presence of optimal concentrations of granulocyte / macrophage colony stimulating factor ( GM CSF ) + interleukin ( IL ) 3 + IL 6 + stem cell factor ( SCF ) + Flt 3 ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The influence of interleukin ( IL ) 4 , IL 13 , and Flt 3 ligand on human dendritic cell differentiation from cord blood CD34+ progenitor cells . ^^^ The effect of Flt 3 ligand ( FL ) , known to enhance proliferation of hematopoietic progenitors induced by other growth factors , was examined next . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
FLT 3 ligand inhibits apoptosis and promotes survival of myeloid leukemia cell lines . ^^^ Here , we analyzed the survival promoting and apoptosis inhibiting properties of FLT 3 ligand ( FL ) using the viable cell count as an indicator of programmed cell death . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand does not induce the growth of peripheral blood granulocyte macrophage colony forming cells in myeloproliferative disorders . ^^^ Flt 3 ligand ( FL ) is a growth factor ( GF ) which might have clinical use as a mobilizer of stem and progenitor cells into peripheral blood ( PB ) in autologous transplantations of various malignant haematological diseases , unless FL stimulates the growth of malignant cells in these diseases . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The potential of human mast cell progenitors to differentiate into mature mast cells remains after prolonged culture with flt 3 ligand , interleukin 3 or granulocyte macrophage colony stimulating factor . ^^^ In this study we have investigated the effect of the early acting haemopoietic cytokines flt 3 ligand ( FL ) , IL 3 and GM CSF on the SCF dependent differentiation of mast cells from cord blood mononuclear cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand ( FL ) is an early acting potent co stimulatory cytokine that regulates proliferation and differentiation of a number of blood cell lineages . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Different culture conditions were generated by adding G CSF , granulocyte macrophage colony stimulating factor ( GM CSF ) , Flt 3 ligand ( Flt 3 ) , megakaryocyte growth and development factor ( Peg rHuMGDF ) , GM CSF / erythropoietin ( EPO ) hybrid protein ( MEN 11303 ) , and interleukin 15 ( IL 15 ) to the basic stem cell factor ( SCF ) + interleukin 3 ( IL 3 ) + EPO combination . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Effects of FLT 3 ligand on proliferation and survival of myeloid leukemia cells . ^^^ FLT 3 ligand ( FL ) acting through its tyrosine kinase receptor FLT 3 has pleiotropic and potent effects on hematopoietic cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Mechanism of flt 3 ligand expression in bone marrow failure : translocation from intracellular stores to the surface of T lymphocytes after chemotherapy induced suppression of hematopoiesis . ^^^ The flt 3 ligand ( FL ) is a growth factor for primitive hematopoietic cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
This features the use of a serum free medium supplemented with the cytokines flt 3 ligand , IL 3 , stem cell factor ( c kit ligand ) and IL 2 , together with IL 7 or oncostatin M ( OM ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The bone marrow cultures , which included the stromal cells collected with the aspirate , were supplemented with erythropoietin , granulocyte macrophage colony stimulating factor ( GM CSF ) / IL 3 fusion protein ( PIXY 321 ) , and flt 3 ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand can promote survival and macrophage development without proliferation in myeloid progenitor cells . ^^^ Flt 3 ligand elicits a variety of effects on early hemopoietic progenitors by occupying its cognate receptor , Flt 3 , a member of the type 3 tyrosine kinase receptor family . ^^^ Flt 3 ligand had a relatively small proliferative effect on enriched GM CFC compared to SCF and M CSF and had no ability to either stimulate colony formation or synergize with these two cytokines in promoting DNA synthesis , colony formation , or expansion in liquid culture . ^^^ Flt 3 ligand , however , was capable of maintaining the clonogenic potential of GM CFC and acted as an anti apoptotic agent as assessed using the Annexin 5 apoptosis assay . ^^^ GM CFC cultured in Flt 3 ligand eventually formed macrophages and neutrophils in liquid culture . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Minimization of recombinant human Flt 3 ligand aggregation at the Tm plateau : a matter of thermal reversibility . ^^^ This study elucidates the importance of thermal reversibility as it pertains to the minimization of recombinant human Flt 3 ligand aggregation and its potential role for determining solution conditions that can achieve the greatest long term storage stability . ^^^ The findings link thermal reversibility to the propensity of Flt 3 ligand to aggregate once unfolded in the Tm plateau region and provide a basis for relating the reversibility of thermal denaturation to the prediction of long term storage stability in aqueous solution . . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand : role in control of hematopoietic and immune functions of the bone marrow . ^^^ Potent effects of flt 3 ligand on HSCs and the development of the immune system have generated much interest in the clinical application of this cytokine in stem cell transplantation and cancer immunotherapy . . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
METHODS : AC 133 ( + ) stem / progenitor cells from six full term pregnancies UCB samples were immunomagnetically selected , followed by ex vivo expansion culture in the presence of thrombopoietin ( TPO ) , c kit ligand ( KL ) , flt 3 ligand ( FL ) and IL 6 . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
However , the Flt 3 ligand had no significant effect on self renewal of the Flt 3 ( + ) HSCs . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Furthermore , over 8 weeks in liquid culture stimulated by a cytokine cocktail optimized for HSPC expansion , TPOFLK ( thrombopoietin 10 ng / ml , Flt 3 ligand 50 ng / ml , c Kit ligand 20 ng / ml ) Lin cells underwent slow proliferation but maintained / expanded more primitive HSPC than CD133+ cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
RESULTS : Proliferation by CFU GM and BFU E was enhanced synergistically when common beta chain receptor cytokines ( IL 3 or GM CSF ) were combined with G CSF or EPO , but not with gp 130 receptor cytokines ( LIF or IL 6 ) or tyrosine kinase receptor cytokines ( SCF , HGF , Flt 3 ligand , or PDGF ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The phenotype and function of splenic DC populations from diabetes prone NOD mice were characterized and compared to DC from diabetes resistant strains in the presence or absence of Flt 3 ligand ( FL ) treatment . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Reconstitution of dendritic and natural killer cell subsets after allogeneic stem cell transplantation : effects of endogenous flt 3 ligand . ^^^ The speed of recovery of CD11c ( + ) CD 123 ( low ) DC 1 exceeded that of CD11c ( ) CD 123 ( + ) DC 2 , and correlated with plasma levels of flt 3 ligand ( FL ) , but not with granulocyte or granulocyte macrophage colony stimulating factors and stem cell factor . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Safety and biological activity of repeated doses of recombinant human Flt 3 ligand in patients with bone scan negative hormone refractory prostate cancer . ^^^ PURPOSE : The purpose of this study was to evaluate the safety , biological activity , and feasibility of repeated doses of the dendritic cell ( DC ) stimulating agent Flt 3 ligand ( FL ) in patients with bone scan negative hormone refractory prostate cancer . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
SJL and BALB / c marrow cells were serially cultured with thrombopoietin ( TPO ) , FLT 3 ligand ( FLT 3L ) , and steel factor through cell cycle . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
FLT 3 ITD receptors exhibit constitutive tyrosine kinase activity in the absence of FLT 3 ligand ( FL ) binding , and when expressed in cytokine dependent cell lines and primary hematopoietic cells suppress programmed cell death and increase cell division . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
CpG activation of tumoral DCs alone was not sufficient to induce tumor regression in either tumor model , nor was systemic delivery of the DC growth factor , Flt 3 ligand , which dramatically increased the number of circulating DCs but not the number of tumoral DCs . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In serum free single cell suspension culture , VIP enhanced clonal growth of CD34+ CB cells in synergy with FLT 3 ligand ( FL ) , stem cell factor ( SCF ) , and thrombopoietin ( TPO ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Nasal Flt 3 ligand cDNA elicits CD11c+CD8+ dendritic cells for enhanced mucosal immunity . ^^^ In this study , we assessed a cDNA vector for Flt 3 ligand ( FL ) for its potential to enhance mucosal immunity or tolerance . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
To determine whether greater DC numbers promoted T cell mediated protection in the context of host defense against intracellular bacteria , we treated mice with Flt 3 ligand ( Flt 3 L ) to increase DCs in vivo and challenged them with Listeria monocytogenes . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand preferentially increases the number of functionally active myeloid dendritic cells in the lungs of mice . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In culture with stem cell factor , FLT 3 ligand , and IL 6 , a 4 hydroxytamoxifen inducible form of c Myc ( Myc / ERT ) enabled murine Lin ( ) Sca 1 ( + ) HSCs to proliferate with the surface phenotype compatible with HSCs for more than 28 days . c Myc activated by 4 hydroxytamoxifen augmented telomerase activities and increased the number of CFU Mix about 2 fold in colony assays . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand reverses late allergic response and airway hyper responsiveness in a mouse model of allergic inflammation . ^^^ Flt 3 ligand ( Flt 3 L ) is a growth factor for dendritic cells and induces type 1 T cell responses . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
IMC EB 10 blocks the binding of FLT 3 ligand ( FL ) to soluble FLT 3 in ELISA and competes with FL for binding to cell surface FLT 3 receptor . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Heart , but not skin , allografts from donors lacking Flt 3 ligand exhibit markedly prolonged survival time . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Under these conditions c kit ligand , interleukin 7 ( IL 7 ) , and Flt 3 ligand ( Flt 3 L ) are added to the cultures to ensure optimal B cell differentiation . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
FLT 3 ligand regulates apoptosis through AKT dependent inactivation of transcription factor FoxO 3 . ^^^ FLT 3 ligand ( FL ) is one of the most important cytokines for regulation of the hematopoietic system , and its receptor FLT 3 is expressed on both stem cells and progenitors . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
To identify putative predictors of response , patient plasma was analyzed for levels of secreted ligands of SU 5416 targets ; SCF and FLT 3 ligand . ^^^ Baseline levels of SCF in patients with stable or progressive disease were significantly higher than those in normal donors , whereas FLT 3 ligand levels in patients who exhibited progressive disease were significantly lower than those in normal donors . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Autocrine stimulation of wild type ( WT ) FLT 3 by coexpressed FLT 3 ligand ( FL ) occurs in many other cases . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Hematopoietic stem cells mitogens SCF and flt 3 ligand ( FL ) were also mitogenic for fetal hepatocytes , but did not further enhance the effect of IL 6c on cell proliferation . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand bioactivity and pharmacology in neoplasia . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Cord blood derived purified CD34+ cells were grown in liquid medium supplemented with the cytokines stem cell factor , thrombopoietin , Flt 3 ligand , and IL 6 , and the chelator TEPA for the first 3 weeks and then for up to 11 weeks with cytokines alone . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Both soluble and membrane bound forms of Flt 3 ligand enhance tumor immunity following `` suicide ' ' gene therapy in a murine colon carcinoma model . ^^^ The ability of the dendritic cell stimulatory cytokine Flt 3 ligand ( Flt 3 L ) to modulate prodrug activated gene therapy has been investigated . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Treatment of neonatal mice with Flt 3 ligand leads to changes in dendritic cell subpopulations associated with enhanced IL 12 and IFN alpha production . ^^^ Treatment with the hematopoietic growth factor Flt 3 ligand ( FL ) increases DC numbers in neonatal mice and enhances their resistance against intracellular pathogens . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Expansion of CIK / NK cells from cord blood by using different combinations of stem cell factor , FLT 3 ligand and interleukin 2 , 7 , 15 in vitro ] . ^^^ To explore the efficacy of the different combinations of stem cell factor ( SCF ) , FLT 3 ligand ( FL ) and interleukin ( IL ) 2 , 7 , 15 on the induction and expansion of cord blood ( CB ) derived CIK / NK cells in vitro , according to the different combinations of cytokines , combinations of cytokines were divided into 3 groups : group A ( SCF + IL 2 + IL 7 + IL 15 ) , group B ( SCF + FL + IL 2 + IL 7 + IL 15 ) and group C ( IL 2 + IL 7 + IL 15 as control group ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Coexpression of FLT 3 ligand did not improve the viability , expansion , or immunologic performance of lentivirus modified DCs . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Effect of addition of FLT 3 ligand and megakaryocyte growth and development factor on hemopoietic cells in serum free conditions . ^^^ We report the results of experiments with CD 34 ( + ) cells from cord blood ( CB , n = 13 ) , bone marrow ( BM , n = 4 ) , and mobilized peripheral blood stem cells ( PBSC , n = 5 ) using two combinations of cytokines : ( A ) granulocyte colony stimulating factor ( G CSF ) , interleukin 3 ( IL 3 ) , interleukin 6 ( IL 6 ) , stem cell factor ( SCF ) , erythropoietin ( EPO ) , insulin like growth factor 1 ( IGF 1 ) , basic fibroblast growth factor ( FGF b ) and ( B ) combination A plus FLT 3 ligand ( FL ) and megakaryocyte growth and development factor ( PEG rhMGDF ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The impact of imiquimod , a Toll like receptor 7 ligand ( TLR7L ) , on the immunogenicity of melanoma peptide vaccination with adjuvant Flt 3 ligand . ^^^ Flt 3 ligand ( FL ) is a hematopoietic growth factor that increases the number of immature DCs in the blood and other tissues . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The DC promoting effect of vitamin A was specific to myeloid lineage development stimulated by GM CSF because vitamin A significantly inhibited DC development stimulated by flt 3 ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
To overcome this limitation , we developed an ex vivo expansion system for HSC , in which CB CD34+ cells are cultured on feeder cells ( HESS 5 cells ) in the presence of cytokines ( TPO , SCF and Flt 3 ligand ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The omentum stroma can supply in vitro the cytokines ( SDF 1alpha , Flt 3 ligand and IL 7 ) and the molecular environment required for generation of these two cell lineages . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In the first step , CB CD34+ cells were expanded by stimulation with early acting cytokines such as stem cell factor ( SCF ) , flt 3 ligand ( FL ) , and thrombopoietin ( TPO ) to amplify the hematopoietic progenitor cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
DNA vaccines and dendritic cell potentiators , such as CpG oligodeoxynucleotides and Flt 3 ligand , are also in the early stage of development . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
New efforts focus on blocking the binding of FLT 3 ligand to its receptor as a means of inhibiting autocrine stimulation in leukemogenesis . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand and the Flt 3 receptor regulate hematopoietic cell migration by modulating the SDF 1alpha ( CXCL 12 ) / CXCR4 axis . ^^^ Flt 3 ligand ( FL ) enhances hematopoietic cell proliferation and facilitates hematopoietic stem cell mobilization in vivo , while the stromal derived factor 1alpha ( SDF 1alpha , CXC ligand 12 [ CXCL 12 ] ) / CXC receptor 4 ( CXCR 4 ) axis is critical for their homing and trafficking . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
FLT 3 ligand administration after hematopoietic cell transplantation increases circulating dendritic cell precursors that can be activated by CpG oligodeoxynucleotides to enhance T cell and natural killer cell function . ^^^ FLT 3 ligand ( FLT 3 L ) is essential for DC development from hematopoietic progenitors . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Retroviral transduction of CD 34 ( + ) hemopoietic progenitor cells , during pre expansion by thrombopoietin , c kit ligand , and FLT 3 ligand , on recombinant fibronectin , but in the absence of FCS , resulted in gene expression by 20 30 % of the LCs . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Treatment with Flt 3 ligand plasmid reverses allergic airway inflammation in ovalbumin sensitized and challenged mice . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
METHODS : Enriched CD34+ cells were cultured for 8 or 10 d in serum free media ( QBSF 60 or 10 Vivo 10 ) and four combinations of cytokines consisting of recombinant human pegylated megakaryocyte growth and development factor , stem cell factor , flt 3 ligand , G CSF , interleukin ( IL ) 6 , platelet derived growth factor ( PDGF ) , and IL 1beta . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
FCs and p preDCs share many phenotypic , morphological , and functional features : both produce IFN alpha and TNF alpha , both are activated by toll like receptor ( TLR ) 9 ligand ( CpG ODN ) stimulation , and both expand and mature after Flt 3 ligand ( FL ) treatment . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
BM mononuclear cells from 3 dogs with melanoma and from 1 healthy dog were cultured for 12 days in media supplemented with recombinant human granulocyte macrophage colony stimulating factor , stem cell factor , tumor necrosis factor , and Flt 3 ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
To address this problem we developed a method for loading and activating DCs , in situ , by first directing sufficient numbers of DCs to peripheral tissues using Flt 3 ligand and then delivering a tumor associated Ag and oligonucleotide containing unmethylated CG motifs to these tissues . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
This study examined the effects of mesenchymal stem cells ( MSCs ) on the maintenance of CD34+ cells driven by aza D and TSA in culture with the combined cytokines of thrombopoietin , flt 3 ligand , stem cell factor , interleukin 3 , and interleukin 6 . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
All patients received systemic Flt 3 ligand ( 20 microg / kg / d ) and i . d . peptides : Three NY ESO 1 peptides , SLLMWITQCFL ( 157 167 ) , SLLMWITQC ( 157 165 ) , QLSLLMWIT ( 155 163 ) ; tyrosinase internal peptide YMDGTMSQV ( 368 376 ) ; Melan A / MART 1 analogue peptide ELAGIGILTV ( 26 35 , E27L substitution ) ; and influenza matrix peptide GILGFVFTL ( 58 66 ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
METHODS : CD34+CD38 Lin cells from UCB were cultured in transwells above AFT 024 in the presence of Flt 3 ligand ( FLT ) and thrombopoietin ( TPO ) , with or without ATRA . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Within the first 6 hours post lethal dose radiation , there is no significant change of gene expression of the known radioprotective factors TPO , SCF , IL 3 , Flt 3 ligand , and SDF 1 . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
RESULTS : Flt 3 ligand stimulation of wild type Flt 3 increased phosphorylation of Src kinase Lyn . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Radiation induced increase in plasma Flt 3 ligand concentration in mice : evidence for the implication of several cell types . ^^^ Circulating T lymphocytes were proposed as the main producer of Flt 3 ligand . ^^^ However , during aplasia , there is a drastic reduction in the number of T lymphocytes , while plasma Flt 3 ligand concentration is increased . ^^^ This contradiction prompted us to compare variations in plasma Flt 3 ligand during radiation induced aplasia in BALB / c mice and in T lymphocyte deficient NOD SCID mice to delineate the role of T lymphocytes in the increase in Flt 3 ligand concentration . ^^^ The results showed that plasma Flt 3 ligand concentration was increased similarly in the two strains of mice , and that Flt 3 ligand concentration was negatively correlated to the number of residual hematopoietic progenitors . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Test cells also show increased expression of FLT3R and improved responsiveness to FLT 3 ligand , the homing related cytokine . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Forty three cases of childhood acute myeloid leukemia ( AML ) and 27 cases of childhood acute lymphocytic leukemia ( ALL ) were examined by flow cytometric analysis for cell surface flt3R and proliferative response in vitro to flt 3 ligand ( flt3L ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In the present study , daily injection of human Flt 3 ligand ( Flt3L ) into mice results in a dramatic numerical increase in cells co expressing the characteristic DC markers class 2 MHC , CD11c , DEC 205 , and CD 86 . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand ( flt3L ) is a member of a small family of cytokines acting as tyrosine kinase receptor ligands that stimulate the proliferation of primitive hematopoietic progenitors in vitro . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
To determine the value of flt 3 ligand ( flt3L ) in stimulating hematopoiesis in human hypoproliferative bone marrow disorders , we examined its in vitro effect on bone marrow cells from patients with aplastic anemia ( AA ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The ligand for flt 3 ( FLT3L ) exhibits striking structural homology with stem cell factor ( SCF ) and monocyte colony stimulating factor ( M CSF ) and also acts in synergy with a range of other hematopoietic growth factors ( HGF ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand ( flt3L ) has potent effects on hemopoietic progenitors , dendritic cells , and B lymphopoiesis . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The hematopoietic growth factors stem cell factor ( SCF ) and flt 3 ligand ( flt3L ) are produced within the hematopoietic microenvironment in a membrane bound and soluble isoform . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Whilst samples without a mutation of the Flt 3 gene showed an increased cell production in response to either IL 3 and G CSF or IL 6 , SCF , TPO and Flt3L in long term stroma supported cultures , mutant samples failed to do so . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Our work has been concerned with the detailed characterization of four distinct DC populations in NOD mice : two derived from bone marrow ( BM ) cells cultured in either granulocyte macrophage colony stimulating factor ( GM CSF ) plus interleukin 4 ( IL 4 ) or GM CSF alone and two from the spleen of Flt 3 ligand ( Flt3L ) treated mice , isolated on the basis of CD8alpha expression . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
To delineate factors involved in NK cell development , we established an in vitro system in which lineage marker ( Lin ) , c kit+ , Sca2+ bone marrow cells differentiate into lytic NK1 . 1+ but Ly 49 cells upon culture in IL 7 , stem cell factor ( SCF ) , and flt 3 ligand ( flt3L ) , followed by IL 15 alone . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In the present study we investigated whether DC can be converted from tolerogenic into immunogenic APC by treating mice with Flt 3 ligand ( Flt3L ) , a DC growth factor , and then immunizing with CT . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In 2 animals , cells of the different fractions were transduced in the presence of human FLT 3 ligand ( FLT3L ) , canine stem cell factor ( cSCF ) , and human megakaryocyte growth and development factor ( MGDF ) , and in 2 other dogs , transduction was performed in the presence of FLT3L , cSCF , and canine granulocyte colony stimulating factor ( cG CSF ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
We describe here that lineage phenotype negative ( Lin ) ( ) c kit ( + ) hematopoietic progenitor cells ( HPCs ) from day 13 postcoitus ( dpc ) murine fetal liver ( FL ) can generate dendritic cell ( DC ) precursors when cultured in vitro in the presence of PA 6 stromal cells plus granulocyte / macrophage colony stimulating factor ( GM CSF ) + stem cell factor ( SCF ) + Flt 3 ligand ( Flt3L ) for 12 to 14 days , and develop into mature DCs when stimulated with GM CSF plus mouse tumor necrosis factor alpha ( mTNFalpha ) for an additional 3 to 5 days . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The flt 3 ligand ( flt3L ) is a growth factor for hematopoietic progenitors and induces hematopoietic progenitor and stem cell mobilization in vivo . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
To investigate the role of DC in regulating the homeostatic balance between mucosal immunity and tolerance , we treated mice with Flt 3 ligand ( Flt3L ) , a growth factor that expands DC in vivo , and assessed subsequent systemic immune responsiveness using mouse models of oral tolerance . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
FLT 3 ligand ( FLT3L ) stimulates primitive hematopoietic cells by binding to and activating the FLT 3 receptor ( FLT3R ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
When the hematopoietic factor Flt 3 ligand ( Flt3L , 40 ng ; ml ) was added , there was an average increase in the DC population of 26 % compared to cultures with GM CSF and IL 4 alone . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
We therefore analyzed the effect of Flt 3 ligand ( Flt3L ) and of CD 40 ligand on the development of human DC from blood monocytes in the absence of GM CSF . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Liposomes and Flt 3 ligand ( Flt3L ) , a ligand for the fms like tyrosine kinase receptor Flt3 / FLK 2 , can augment the immune response to an HIV peptide vaccine . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
PURPOSE : To evaluate preoperative dendritic cell ( DC ) mobilization and tumor infiltration after administration of Flt 3 ligand ( Flt3L ) to patients with metastatic colon cancer . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
To determine whether immune stimulation could reduce acute myelogenous leukemia ( AML ) lethality , dendritic cells ( DCs ) were pulsed with AML antigens and used as vaccines or generated in vivo by Flt 3 ligand ( Flt3L ) , a potent stimulator of DC and natural killer ( NK ) cell generation . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand ( Flt3L ) plays a critical role in the proliferation , differentiation and survival of haematopoietic progenitor cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
OBJECTIVE : Mechanisms of T cell stimulation by Flt 3 ligand ( Flt3L ) and granulocyte macrophage colony stimulating factor ( GM CSF ) remain unclear . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
We found that tumor protection and an antigen ( Ag ) specific immune response were enhanced by prior injection of Flt 3 ligand ( Flt3L ) at a dose and schedule that significantly increased dendritic cell ( DC ) number and frequency . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Mobilization of mice with Flt 3 ligand ( Flt3L ) or Flt3L and granulocyte macrophage colony stimulating factor , hematopoietic factors that specifically enhance DC growth , resulted in strikingly increased numbers of pDC in bone marrow and spleen . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand ( Flt3L ) potently induced equal expansion of both subsets in vivo . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Human Flt 3 ligand ( Flt3L ) stimulates early hematopoiesis by activating a type 3 tyrosine kinase receptor on primitive bone marrow stem cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In this study the effects of five immunomodulators ; recombinant CD 40 ligand ( CD40L ) , bryostatin 1 , recombinant FLT 3 ligand ( FLT3L ) , recombinant granulocyte colony stimulating factor ( G CSF ) and recombinant interleukin 15 ( IL 15 ) were investigated against PCP in a dexamethasone immunosuppressed Sprague Dawley rat model . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
We tried to establish , whether pre treatment serum levels of the angiogenic factors VEGF , bFGF , interleukin 8 ( IL 8 ) and Flt 3 ligand ( Flt3L ) correlate with prognostic variables in 30 patients with primary gastro intestinal non Hodgkin ' s lymphoma . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The adjuvant activity of Flt 3 ligand ( Flt3L ) and conjugation to an interleukin ( IL ) 1beta bioactive fragment were compared , either alone or in combination , for their ability to induce T and B cell responses to the HGP 30 peptide sequence ( amino acids 86 115 of human immunodeficiency virus ( HIV ) gag p 17 ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The analysis also revealed that normal villus epithelium expresses Fms like tyrosine kinase 3 ( Flt 3 ) , a known regulator of hematopoietic stem cell proliferation and neuronal cell survival , and its ligand ( Flt3L ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Endogenous FLT 3 ligand serum levels are associated with disease stage in patients with myelodysplastic syndromes . ^^^ The FLT 3 ligand , which binds to and activates the FLT 3 receptor , does not have a stimulatory effect on hematopoietic cells , but can synergize with other HGF to support the expansion of both immature and committed progenitors . ^^^ Using ELISA technology we measured endogenous serum levels in 93 patients with MDS : 29 RA , 1 RARS , 31 RAEB , 23 RAEBt , 9 CMML . 48 . 3 % of RA patients ' sera had significantly elevated FLT 3 ligand levels ranging from 404 to 5735 pg / ml , whereas none of the RAEB , RAEBt , or CMML patients sera had levels different from controls . ^^^ No significant correlation was found between FLT 3 ligand levels and peripheral blood counts , bone marrow cellularity , age , cytogenetic abnormalities , or survival . ^^^ Our data suggest that FLT 3 ligand levels can be upregulated early in the course of MDS , which may represent an appropriate response to a decreased number of normal progenitors , or alternatively a dysregulated HGF system . . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Retroviral transduction with the MFG EGFP retroviral vector resulted in readily detectable EGFP expression in 27 % of human and 11 35 % of rhesus monkey bone marrow cells , and in 17 38 % of rhesus monkey peripheral blood cells mobilized with FLT 3 ligand ( FL ) and granulocyte colony stimulating factor ( G CSF ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In the present study , this problem has been addressed by expanding DC in vivo using flt 3 ligand , followed by the purification of CD11c+ cells using antibody coated magnetic beads or by fluorescence activated cell sorting . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In a murine model , we analysed the in vitro effects of granulocyte macrophage colony stimulating factor ( GM CSF ) alone or combined with interleukin 4 ( IL 4 ) or Flt 3 ligand ( Flt 3 L ) on the number , immunophenotype and functions of bone marrow derived DC . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Because Flt 3 ligand ( FL ) enhances the growth and / or maintenance of primitive cells , it was important to know how long early cells retain Flt 3 receptor expression in expansion culture . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
To characterize the fate of early hematopoietic progenitor cells during ex vivo expansion in suspension culture , human CD 34 ( + ) enriched BPC were cultured in serum free medium in the presence of FLT 3 ligand ( FL ) , stem cell factor ( SCF ) and interleukin 3 ( IL 3 ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Impact of Flt 3 ligand on donor derived antigen presenting cells and alloimmune reactivity in heart graft recipients given adjuvant donor bone marrow . ^^^ The influence of the haematopoietic growth factor Flt 3 ligand ( FL ) on the incidence and function of donor major histocompatibility complex ( MHC ) class II+ cells in the lymphoid tissues of noncytoablated recipients of heart allografts and donor bone marrow ( BM ) cells was investigated . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
RESULTS : Under our optimized conditions of mouse BM incubation ( stem cell factor plus interleukin 11 , either with or without macrophage inflammatory protein 1alpha or Flt 3 ligand ) , both the short term and the mid term repopulating ability of the ex vivo expanded samples were significantly improved when compared with fresh samples . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
These studies indicate that all factors examined , including vascular endothelial growth factor and its receptor FLK 1 , Flt 3 ligand and its receptor STK 1 , and stem cell leukemia transcription factor , are expressed by both hematopoietic cells in the cluster and endothelial cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
MPB purified CD34+ Thy 1+ cells were labeled with CFSE dye and cultured for 112 hours in serum deprived medium in the presence of the cytokine combinations of thrombopoietin ( TPO ) , flt 3 ligand ( FL ) , and c kit ligand ( KL ) , or TPO , FL , and interleukin 6 ( IL 6 ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Comparing several different combinations of cytokines added to 90 hour cultures of MPB CD 34 cells , thrombopoietin ( TPO ) , flt 3 ligand ( FL ) , and c kit ligand ( KL ) gave the best result , with the lowest percentage of apoptotic cells and a mean 1 . 2 fold increase in the number of CD34+ Thy 1+ cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
We examined the effect of recombinant human interleukin ( IL ) 11 alone or in combination with various colony stimulating factors ( CSFs ) , including IL 3 , granulocyte / macrophage ( GM ) CSF , granulocyte ( G ) CSF , stem cell factor ( SCF ) , flt 3 ligand ( FL ) , and thrombopoietin ( TPO ) , on colony formation by leukemic progenitor cells ( L CFU ) obtained from 33 patients with acute myelogenous leukemia ( AML ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The addition of flt 3 ligand to cultures increased the transduction rate of LTC ICs . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Therefore , we have attempted to maintain human cord blood or bone marrow CD 34 ( + ) cells ex vivo in serum free cultures containing stem cell factor ( SCF ) and FLT 3 ligand ( FL ) alone or together with a new recombinant molecule of soluble IL 6 receptor fused to IL 6 ( IL6RIL6 chimera ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In short term cultures containing erythropoietin ( Epo ) , interleukin ( IL ) 1 , IL 3 , and IL 6 , or granulocyte ( G ) and granulocyte macrophage colony stimulating factor ( GM CSF ) together with stem cell factor ( SCF ) or Flt 3 ligand ( FL ) , expansion of CFUs was six to eightfold at week 1 . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Compared with interleukin 3 ( IL 3 ) , IL 6 , and leukemia inhibitory factor ( LIF ) , the combination of thrombopoietin ( TPO ) , Flt 3 ligand ( FL ) , and Kit ligand ( KL ) effected a 73 fold increase in NGFR expression among CD34+ cells ( to 14 % ) and a 14 fold increase in NGFR expression among total cells ( to 10 % ) after LTC . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Generating a T cell tumor specific immune response in vivo : can flt 3 ligand generated dendritic cells tip the balance . flt 3 ligand ( FL ) is a growth factor that induces hematopoietic progenitor cell and dendritic cell ( DC ) expansion when administered to mice . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Effect of vascular endothelial growth factor and FLT 3 ligand on dendritic cell generation in vivo . ^^^ The cytokine FLT 3 ligand ( FL ) enhances dendritic cell ( DC ) generation and has therefore been proposed as a means to boost antitumor immunity . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Nevertheless , approximately 1 % of the same lin ( ) CD 34 ( ) CD 38 ( ) cells were able to proliferate rapidly in serum free liquid suspension cultures containing human flt 3 ligand , Steel factor , thrombopoietin , interleukin 3 ( IL 3 ) , and hyper IL 6 to produce a net 28 + / 8 fold increase in total cells within 10 days . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Modulation of haematopoietic progenitor development by FLT 3 ligand . ^^^ To increase our knowledge of the functional properties of the human Flt 3 ligand ( FL ) as relating to in vitro expansion of haematopoietic stem cells , the effects on murine haematopoiesis of FL alone or in combination with other growth factors were studied . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In this study , mouse DC were generated from CD34+ bone marrow precursors and cultured with granulocyte macrophage CSF and Flt 3 ligand for 9 days . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand antitumor activity in a murine breast cancer model : a comparison with granulocyte macrophage colony stimulating factor and a potential mechanism of action . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The reduction of in vitro radiation induced Fas related apoptosis in CD34+ progenitor cells by SCF , FLT 3 ligand , TPO , and IL 3 in combination resulted in CD34+ cell proliferation and differentiation . ^^^ The early effects of exogenous cytokines at the hematopoietic stem and progenitor cell ( HSPC ) level following irradiation are still largely unknown , especially those of survival factors such as stem cell factor ( SCF ) and Flt 3 ligand ( FL ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Here , we show that B lineage cells generated in vitro from ES cells are functionally analogous to normal fetal liver derived or bone marrow derived B lineage cells at three important developmental stages : first , they respond to Flt 3 ligand during an early lymphopoietic progenitor stage ; second , they become targets for Abelson murine leukemia virus ( A MuLV ) infection at a pre B cell stage ; third , they secrete Ig upon stimulation with lipopolysaccharide at a mature mitogen responsive stage . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In the presence of early acting cytokines interleukin 1beta ( IL 1beta ) , IL 3 , IL 6 , stem cell factor ( SCF ) , flt 3 ligand with and without thrombopoietin ( Tpo ) , we compared the expansion capacity of CD34+ enriched cells from CB , NB and bone marrow ( BM ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
NALM 29 cells responded significantly to the proliferative stimuli of FLT 3 ligand and IL 7 , but not to GM CSF , IL 3 , IL 6 , PIXY 321 or SCF . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Medium for the ex vivo cultures contained either serum and six recombinant human hematopoietic growth factors ( GFs ) , including Flt 3 ligand ( FL ) , Kit ligand ( KL = stem cell factor ) , thrombopoietin ( Tpo ) , interleukin 3 ( IL 3 ) , granulocyte colony stimulating factor ( G CSF ) , and interleukin 6 ( IL 6 ) , or a serum free medium containing only FL , KL , and Tpo . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The transduction protocol used the CH 296 recombinant human fibronectin fragment and relatively high concentrations of the flt 3 ligand and stem cell factor . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The in vitro radiation sensitivity of CFU Meg isolated from human placental and umbilical cord blood was evaluated in plasma clot cultures stimulated by recombinant human cytokines , including thrombopoietin , the FLT 3 ligand ( FLT3LG ) , interleukin 3 , interleukin 11 and stem cell factor . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand , in combination with bovine granulocyte macrophage colony stimulating factor and interleukin 4 , promotes the growth of bovine bone marrow derived dendritic cells . ^^^ Culture of bone marrow precursor cells with cytokines , including granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin 4 ( IL 4 ) and the tyrosine kinase receptor binding proteins Flt 3 ligand ( Flt 3L ) and stem cell factor ( SCF ) , has previously been shown , in both mouse and human , to result in the generation of large numbers of dendritic cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand induces the outgrowth of Mac 1+B220+ mouse bone marrow progenitor cells restricted to macrophage differentiation that coexpress early B cell associated genes . ^^^ Flt 3 ligand ( FL ) is an important cytokine that affects the proliferation of hematopoietic stem cells and multipotent progenitors . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In contrast , KL , flt 3 ligand ( FL ) , and megakaryocyte growth and development factor ( MGDF ) ( and KL + FL + IL 3 ) expanded murine long term reconstituting activity as well as human LTC IC and ELTC IC . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Differential regulation of epidermal and dermal dendritic cells by IL 12 and Flt 3 ligand . ^^^ Systemic Flt 3 ligand ( FL ) administration in mice induced a significant increase in mature dendritic cells ( DC ) within the skin , preferentially in the dermis , whereas IL 12 promoted a significant increase of immature DC preferentially in the epidermis . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
DC were grown from bone marrow precursors using a cocktail of GM CSF , IL 4 and Flt 3 ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Based on our earlier study of the conditions required for ex vivo expansion of Lin ( ) CD 34 ( + ) CD 38 ( ) cells and SRC , CD 34 ( + ) enriched lineage depleted umbilical cord blood cells were cultured for 2 to 6 days on fibronectin fragment in MGIN ( MSCV EGFP Neo ) retroviral supernatant ( containing 1 . 5 % fetal bovine serum ) and IL 6 , SCF , Flt 3 ligand , and G CSF . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In the experiments with primary human cells , equal aliquots of enriched CD34+ cord blood cells were first stimulated for 2 days with cytokines ( Flt 3 ligand , Steel factor , IL 3 , IL 6 , and G CSF ) and then exposed for 4 days to a green fluorescent protein ( GFP ) and Neo ( r ) encoding retrovirus produced in PG 13 cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Cord blood CD34+ cells were transduced to high levels over 24 48 hr in the presence of Flt 3 ligand , stem cell factor , interleukin 3 , and interleukin 6 . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
A large number of cells with the morphological , phenotypical , and functional characteristics of DCs was generated from both c kit ( low ) and c kit ( low ) populations when cultured with a combination of cytokines ( GM CSF , tumor necrosis factor a [ TNF a ] , interleukin 7 [ IL 7 ] , IL 3 , stem cell factor [ SCF ] , and flt 3 ligand ) ; the cytokine combination studies revealed that SCF and IL 3 in addition to GM CSF and TNF a are essential for DCs to be generated from these primitive populations . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Immunoselected CD34+ cells from peripheral blood stems cells ( PBSCs ) were cultured in media with or without serum , supplemented by IL 3 , IL 6 , IL 11 , SCF , TPO , Flt 3 ligand , and MIP 1alpha . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand ( FL ) and its influence on immune reactivity . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
PATIENTS AND METHODS : The therapeutic potential of dendritic cells and the role of FLT 3 ligand , a potent hematopoietic growth factor , was investigated in a variety of preclinical models . ^^^ RESULTS : In a murine liver metastases model , FLT 3 ligand administration alone or in combination with IL 12 or IL 2 had significant antitumor effects and resulted in significant infiltration of the tumor border by lymphocytes and dendritic cells , which was associated with an increased number of apoptotic figures . ^^^ CONCLUSION : These studies confirm the feasibility of immunotherapeutic approaches using dendritic cells and FLT 3 ligand and demonstrate their potential antitumor activity . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Dendritic cells generated from CD34+ progenitor cells with flt 3 ligand , c kit ligand , GM CSF , IL 4 , and TNF alpha are functional antigen presenting cells resembling mature monocyte derived dendritic cells . ^^^ The combination of cytokines flt 3 ligand + c kit ligand + granulocyte macrophage colony stimulating factor ( GM CSF ) + interleukin 4 ( IL 4 ) + tumor necrosis factor alpha ( TNF alpha ) was used to maximize the output of mature DCs in the culture of CD34+ cells while minimizing the production of monocytes . ^^^ Differentiation was marked by a time dependent increase in allostimulatory function , which , at its peak , was more potent than in cultures of DCs generated from monocytes with GM CSF + IL 4 , but was comparable on a cell to cell basis to that of mature monocytes cultured in flt 3 ligand + c kit ligand + GM CSF + IL 4 + TNF alpha . ^^^ Altogether , these results suggest that DCs generated from CD34+ cells of patients with breast cancer with flt 3 ligand , c kit ligand , GM CSF , IL 4 , and TNF alpha are competent antigen presenting cells , particularly for CD8+ cytotoxic T lymphocytes , and resemble mature monocyte derived DCs in the assays described here . . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Mutant Flt 3 transfected cells exhibited autonomous growth while wild type Flt 3 transfected cells with the continuous stimulation of Flt 3 ligand exhibited a minimal proliferation . ^^^ In contrast , Flt 3 ligand stimulation caused rapid activation of MAP kinase but not STAT 5 in cells expressing wild type Flt 3 . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
A combination of stem cell factor , Flt 3 ligand and IL 7 was also able to support the proliferation and differentiation of the progenitors in a suspension culture . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
We also utilized a two stage culture system to first induce proliferation of Rh ( low ) HSPC by a 48 hour culture in STL + interleukin 6 + Flt 3 ligand , followed by shifting the culture to STL + TGF beta for 24 hours to induce cycle arrest . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
BM was expanded in media containing recombinant flt 3 ligand , erythropoietin , and PIXY 321 , using stromal based perfusion bioreactors for 12 days , and infused after high dose chemotherapy . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The addition of other growth factors such as SCF and Flt 3 ligand can dramatically enhance DC recovery . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Twelve cases of human AML were tested for ability to be differentiated toward a professional APC phenotype in short term culture with addition of GM CSF and the following recombinant proteins : TNFalpha , IL 4 , CD 40 ligand , Flt 3 ligand and SCF . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Intravenous injection of naked DNA encoding secreted flt 3 ligand dramatically increases the number of dendritic cells and natural killer cells in vivo . ^^^ Here we show that hydrodynamics based gene delivery of naked DNA encoding secreted human flt 3 ligand ( hFLex ) can dramatically increase the number of functional DCs and natural killer ( NK ) cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
To test the significance of Flt 3 activation in myeloid cell function , we also characterized the biochemical and biological effects of the activating mutation D838V of Flt 3 ( FLt3D838V ) on the factor dependent myeloid progenitor cell line 32Dcl3 : cells transfected with wild type Flt 3 ( 32D / Flt3 ) grew FLt 3 ligand ( FL ) dependent , and the receptor was ligand dependently autophosphorylated . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Bone marrow aspirate from 5 normal donors and 26 patients with myelodysplastic syndromes were examined for mRNA expression of tumor necrosis factor alpha ( TNF alpha ) , macrophage colony stimulating factor ( M CSF ) , Flt 3 Ligand ( Flt 3L ) , Flt 3 receptor ( Flt 3 rec ) , interleukin 1 beta ( IL 1 beta ) and interleukin 1 receptor antagonist ( IL 1 ra ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Bone marrow pre pro B cells ( AA4 . 1 ( + ) / natural killer 1 . 1 ( ) Fraction A cells ) and cells capable of generating pro B colonies in the presence of interleukin 7 and flt 3 ligand migrate to thymus expressed chemokine ( TECK ) , a response lost in later stages of B cell development . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : CD 34 ( + ) PBPC were cultured in serum free medium comparing different ( n = 32 ) combinations of stem cell factor ( S ) , interleukin 1 ( 1 ) , interleukin 3 ( IL 3 ) ( 3 ) , interleukin 6 ( 6 ) , erythropoietin ( E ) , granulocyte colony stimulating factor ( G ) , granulate macrophage colony stimulating factor ( GM ) , daniplestim ( D , a novel IL 3 receptor agonist ) , and Flt 3 ligand ( FL ) under various culture conditions . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Effects of flt 3 ligand in combination with TPO on the expansion of megakaryocytic progenitors . ^^^ As an early acting growth factor , flt 3 ligand ( FL ) promotes the ex vivo expansion of hematopoietic stem and progenitor cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In the presence of IL 3 , IL 6 , SCF , or leukemia inhibitory factor ( LIF ) and Flt 3 ligand , hMSC facilitated expansion and retroviral transduction of human peripheral blood mobilized CD34+ cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
HPC mobilization by granulocyte colony stimulating factor ( G CSF ) , cyclophosphamide ( CY ) , or interleukin 8 but not flt 3 ligand is markedly impaired in G CSF receptor deficient ( G CSFR deficient ) mice . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The second step of the culture starts at day 6 with the removal of early growth factors in order to allow the differentiation and final maturation of DC during 2 3 weeks of culture with flt 3 ligand ( flt 3L ) and GM CSF . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
FLT 3 ligand gene expression and protein production in human colorectal cancer cell lines and clinical tumor specimens . ^^^ Dendritic cells ( DC ) are professional antigen presenting cells ( APC ) whose proliferation and functional differentiation can be induced by hematopoietic growth factors including GM CSF and FLT 3 ligand ( FL ) . ^^^ In this work we investigated FLT 3 ligand ( FL ) gene expression and protein production in human colorectal cancer cell lines and clinical tumor specimens . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Treatment with Flt 3 ligand , a hematopoietic growth factor that dramatically expands the number of dendritic cells , markedly enhanced the magnitude of virus specific CD 8 ( + ) T cell responses during acute infection and the pool of memory anti polyomavirus CD 8 ( + ) T cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Antileukemic activity of Flt 3 ligand in murine leukemia . ^^^ Flt 3 ligand ( Flt 3 L ) is an early acting costimulatory cytokine that has been shown to possess antitumor properties in murine solid tumor models . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Synergistic combinations of cytokines including TPO , interleukin ( IL ) 1 , IL 3 , IL 11 , stem cell factor , and FLT 3 ligand induce the ex vivo expansion of colony forming unit MK progenitors and MKs from cytokine mobilized peripheral blood cells , bone marrow , and cord blood CD34+ cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Chronic overexpression of membrane bound flt 3 ligand by T lymphocytes in severe aplastic anaemia . ^^^ Aplastic anaemia ( AA ) is an immune mediated bone marrow failure associated with high serum levels of flt 3 ligand ( FL ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Differential effect of interferon alpha on chronic myelogenous leukaemia and normal haematopoietic progenitors in a stromal cell co culture context : role of the flt 3 ligand . ^^^ In co cultures of normal progenitors with EC , IFN alpha increased the generation of clonogenic cells , mainly via an increased production of flt 3 ligand ( FL ) by EC . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Two cytokines , interleukin 7 ( IL 7 ) and Flt 3 ligand ( FL ) , appear to act in conjunction to drive this development process . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Polyethylene glycol modified GM CSF expands CD11b ( high ) CD11c ( high ) but notCD11b ( low ) CD11c ( high ) murine dendritic cells in vivo : a comparative analysis with Flt 3 ligand . ^^^ Daily injection of mice of Flt 3 ligand ( FL ) into mice transiently expands both subsets of DC in vivo , but the effect of administration of GM CSF on the expansion of DC in vivo is not well defined . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand inhibits growth of human ovarian tumors engrafted in severe combined immunodeficient mice . ^^^ OBJECTIVE : The current study evaluated the effects of Flt 3 ligand ( FL ) on the growth of human malignant ovarian tumors engrafted in severe combined immunodeficient ( SCID ) mice with particular attention directed at FL ' s effect on the host natural killer ( NK ) cell response against ovarian cancer xenografts . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Dendritic cells were propagated in response to granulocyte macrophage colony stimulating factor ( GM CSF ) + / interleukin ( IL ) 4 from bone marrow ( BM ) cells or liver non parenchymal cells ( NPC ) isolated from normal mice , or from mice treated with the hematopoietic growth factor Flt 3 ligand ( FL ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand lessens the growth of tumors obtained after colon cancer cell injection in rats but does not restore tumor suppressed dendritic cell function . ^^^ Flt 3 ligand is a cloned hematopoietic growth factor that markedly augments the number of functional dendritic and NK cells in lymphoid and non lymphoid tissues and exerts anti tumor activity in various experimental models . ^^^ We show here that repeated Flt 3 ligand administration delays the s . c . growth of rat colon cancer cells in syngeneic animals without inducing tumor regression . ^^^ In tumor bearing animals , Flt 3 ligand has a limited stimulatory effect on the antigen presenting capacity of intra tumoral and splenic dendritic cells , without restoring the high functional level of dendritic cells from tumor free animals . ^^^ Moreover , Flt 3 ligand mediated activation of NK cell cytotoxicity decreases when the tumor mass increases . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
It transduces signals by ligand mediated dimerization of its receptor , Kit , which is a receptor tyrosine kinase related to the receptors for platelet derived growth factor ( PDGF ) , macrophage colony stimulating factor , Flt 3 ligand and vascular endothelial growth factor ( VEGF ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
CD 135 ( Flk2 / Flt3 ) expression by human thymocytes delineates a possible role of FLT 3 ligand in T cell precursor proliferation and differentiation . ^^^ The expression of the Flk2 / Flt3 molecule ( CD 135 ) , the receptor for Flt 3 ligand ( FL ) , was investigated in the human thymus . ^^^ CD 135 ( Flk2 / Flt3 ) expression by human thymocytes delineates a possible role of FLT 3 ligand in T cell precursor proliferation and differentiation . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Bone marrow and umbilical cord blood CD34+ cells were cultured in serum free medium under survival conditions with thrombopoietin ( Tpo ) alone , or under proliferating conditions with Tpo , c Kit ligand ( KL ) , and Flt 3 ligand ( FL ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Molecular cloning of canine and feline flt 3 ligand reveals high degree of similarity to the human and mouse homologue but uniquely long cytoplasmic domain . ^^^ The cDNAs encoding canine and feline flt 3 ligand ( FL ) have been isolated from libraries constructed from canine or feline peripheral blood mononuclear cells and sequenced . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
We demonstrate here that LC development from human CD34+ hemopoietic progenitor cells in response to TGF beta 1 costimulation ( basic cytokine combination GM CSF plus TNF alpha , stem cell factor , and Flt 3 ligand ) is associated with pronounced cell cluster formation of developing LC precursor cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Microchimerism , donor dendritic cells , and alloimmune reactivity in recipients of Flt 3 ligand mobilized hemopoietic cells : modulation by tacrolimus . ^^^ Flt 3 ligand ( FL ) is a potent hemopoietic growth factor that strikingly enhances stem cells and dendritic cells ( DC ) in vivo . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
By contrast , marked increases in donor liver DC as the result of Flt 3 ligand ( FL ) administration and the resulting augmentation of allostimulatory activity within host lymphoid tissue , is associated with acute graft rejection . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In vivo generation of human dendritic cell subsets by Flt 3 ligand . ^^^ Treatment of mice with human Flt 3 ligand ( FL ) dramatically increases the number of DCs . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Effect of recombinant human FLT 3 ligand ( rhFL ) on in vitro expansion of human cord blood CD34+ cells ] . ^^^ OBJECTIVE : To determine the effects of recombinant human FLT 3 ligand ( rhFL ) on in vitro expansion of human cord blood CD34+ cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Cells were prestimulated with flt 3 ligand , stem cell factor , and interleukin 3 and transduced on fibronectin . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
To delineate the mechanism by which NK cells begin to express different patterns of Ly 49 and CD94 / NKG2 molecules , we developed a clonal assay in which NK1 . 1 ( ) , IL 2 / IL 15 receptor beta+ NK precursors generated by culture of multipotential Lin ( ) , c kit+ progenitors in IL 7 , stem cell factor and flt 3 ligand are induced to differentiate into NK1 . 1+ , Ly49+ NK cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Furthermore , we have ruled out the contribution of 6 known early acting cytokines , including interleukin 3 , interleukin 6 , granulocyte macrophage colony stimulating factor , stem cell factor , flt 3 ligand , and thrombopoietin , to this SCEPF activity . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Through such studies , c kit ligand ( KL ) , flt 3 ligand ( FL ) , thrombopoietin , and IL 11 have emerged as likely positive regulators of HSC self renewal . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Stat5a , but not Stats 1 4 , 5b , or 6 , was potently activated by Flt 3 ligand ( FL ) stimulation . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Finally , novel aspects of stem cell mobilization , including the use of Flt 3 ligand and anti VLA4 / VCAM 1 antibodies , are discussed . . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Interleukin 12 and Flt 3 ligand differentially promote dendropoiesis in vivo . ^^^ Interleukin 12 ( IL 12 ) and Flt 3 ligand ( FL ) regulate hematopoiesis by stimulating proliferation , differentiation and migration of progenitor and / or stem cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
New cytokine / chemokine mobilization regimens are under investigation such as combined rHuG CSF and rHu thrombopoietin , rHuG CSF and interleukin 3 , rHuG CSF and rHu stem cell factor , rHuG CSF and Flt 3 ligand , human macrophage inflammatory protein , interleukin 1 , and interleukin 8 . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In addition , TPO directly and potently synergizes with other early acting cytokines ( kit ligand , flt 3 ligand and interleukin 3 ) to promote multilineage growth of the same progenitor cell population . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Three early acting ( stem cell factor ( SCF ) , Flt 3 ligand ( Flt 3 ) and fetal antigen 1 ( FA 1 ) ) and three lineage specific growth factors ( EPO , G CSF and thrombopoietin ( Tpo ) ) were analyzed by ELISA in 16 patients with multiple myeloma ( MM ) and 16 patients with non Hodgkin ' s lymphoma ( NHL ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Recent studies have reported improved efficiency by including stromal cells ( STR ) , or the fibronectin fragment CH 296 ( FN ) , and various cytokines such as flt 3 ligand ( FLT ) during ex vivo culture and transduction in nonhuman primates . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In this paper the effects of flt 3 ligand ( FL ) , stem cell factor ( SCF ) , interleukin ( IL ) 3 , IL 6 , granulocyte colony stimulating factor ( G CSF ) and granulocyte monocyte colony stimulating factor ( GM CSF ) on long term ex vivo expansion and differentiation of cord blood hematopoietic cells were investigated . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In physical or biological characteristics , it exhibited a unique character different from IL 3 , IL 6 , GM CSF , SCF or FLT 3 ligand those are known to have hematopoietic stem cell proliferation stimulating activity . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Stimulation of mouse bone marrow cells with kit ligand , FLT 3 ligand , and thrombopoietin leads to efficient retrovirus mediated gene transfer to stem cells , whereas interleukin 3 and interleukin 11 reduce transduction of short and long term repopulating cells . ^^^ Large quantitative differences in reconstitution were observed after transplantation with graded numbers ( 1000 30 , 000 ) of EGFP ( + ) cells preconditioned with various combinations of Kit ligand ( KL ) , FLT 3 ligand ( FL ) , thrombopoietin ( TPO ) , interleukin 3 ( IL 3 ) , and IL 11 . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The new promising factor , FLT 3 ligand , combines effect on hematopoiesis with effect on dendritic cells generation . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
HSC were stimulated to proliferate ex vivo by a combination of two growth factors , flt 3 ligand ( FL ) and thrombopoietin / c mpl ligand ( TPO / ML ) , and assessed for expansion by flow cytometry . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Among the cytokine combinations tested , SCF + IL 3 + sIL 6R / IL 6 + flt 3 ligand ( FL ) most effectively expanded CFU GM and CFU Mix . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In cultures with SCF , Flt 3 ligand ( FL ) , granulocyte macrophage colony stimulating factor ( GM CSF ) , and tumor necrosis factor ( TNF ) alpha ( standard condition ) , CD 7 ( + ) CD45RA ( + ) progenitors expanded less ( 37 vs 155 fold ) but yielded 2 fold higher CD1a ( + ) DC percentages than CD 7 ( ) CD45RA ( + ) or CD 7 ( + ) CD45RA ( ) progenitors . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
FLT 3 ligand preserves the uncommitted CD34+CD38 progenitor cells during cytokine prestimulation for retroviral transduction . ^^^ CD34+ cells harvested from umbilical cord blood were kept in suspension cultures and stimulated with combinations of the cytokines stem cell factor ( SCF ) , FLT 3 ligand , interleukin 3 ( IL 3 ) , IL 6 , and IL 7 prior to transduction . ^^^ Then , prestimulation with SCF and FLT 3 ligand was compared . ^^^ Interestingly , FLT 3 ligand seemed to preserve the population of CD34+CD38 cells compared to SCF ( 16 . 56 % + / 2 . 02 versus 9 . 39 % + / 2 . 35 , p = 0 . 03 ) . ^^^ Importantly , the use of FLT 3 ligand seemed to preserve the population of uncommitted cells . . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Comparison of the hematopoietic activity of flt 3 ligand and granulocyte macrophage colony stimulating factor acting alone or in combination . ^^^ The hematopoietic sequelae of intramuscular administration of flt 3 ligand ( FL ) and granulocyte macrophage colony stimulating factor ( GM CSF ) alone , or in combination , were compared in BALB / c mice . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand induces tyrosine phosphorylation of gab 1 and gab 2 and their association with shp 2 , grb 2 , and PI 3 kinase . ^^^ In this study , we show that both Gab 1 and Gab 2 are rapidly tyrosine phosphorylated after Flt 3 ligand stimulation of Flt 3 ligand responsive cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
PMVEC coculture supplemented with GM CSF + IL 3 + IL 6 + SCF + Flt 3 ligand induced > 93 % of the CD 34 ( + ) CD 38 ( ) population to enter the G ( 1 ) or G ( 2 ) / S / M phase while increasing this population from 1 . 4 % on day 0 to 6 . 5 % of the total population by day 5 . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Two conserved bovine flt 3 ligand isoforms , which differ in a defined region within the extracellular domain , were identified and shown to be uniformly transcribed in individuals with diverse MHC haplotypes . ^^^ Notably , at the amino acid level , the extracellular domain of the bovine flt 3 ligand isoform 1 is 81 and 72 % identical with the extracellular domains of the human and murine flt 3 ligands , respectively , whereas isoform 2 has a deletion within this domain . ^^^ Bovine flt 3 ligand isoform 1 , but not 2 , bound the human flt 3 receptor and stimulated murine pro B cells transfected with the murine flt 3 receptor . ^^^ We have shown that a highly conserved , 18 aa sequence within the flt 3 ligand extracellular domain is required for flt 3 receptor binding and function . ^^^ However , a peptide representing this sequence is insufficient for receptor binding as demonstrated by its failure to inhibit the bovine flt 3 ligand isoform 1 binding to the human flt 3 receptor . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The cytokines studied were stem cell factor ( SCF ) , interleukin ( IL ) 3 , megakaryocyte growth and development factor ( MGDF ) , granulocyte colony stimulating factor ( G CSF ) , Flt 3 ligand , IL 6 , IL 11 , and erythropoietin ( EPO ) . ^^^ The effect of Flt 3 ligand and SCF factor on CD34+ cell production was also less than additive , although the response to both cytokines was greater than single cytokines . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Using a 1 day cytokine mediated prestimulation , consisting of human interleukin ( IL ) 3 , IL 6 , stem cell factor ( SCF ) , Flt 3 ligand ( FL ) , and thrombopoietin ( TPO ) , followed by a 3 day transduction procedure , we were able to detect up to 51 % CD 34 ( + ) cells expressing MDR 1 . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
CD34 ( + ) cord blood ( CB ) cells were cultured in serum free medium containing stem cell factor , Flt 3 ligand , megakaryocyte growth and development factor , and granulocyte colony stimulating factor . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
DESIGN AND METHODS : AML blasts were maintained with or without cytokines ( stem cell factor , FLT 3 ligand and interleukin 3 ) for 48 hours , and successively infected with two spin infection cycles . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In this study , it is shown that short term exposure of normal human marrow CD 34 ( + ) CD 38 ( ) cells to low concentrations of tumor necrosis factor ( TNF ) in the presence of 100 ng / mL Flt 3 ligand and Steel factor and 20 ng / mL interleukin 3 ( IL 3 ) , IL 6 , and granulocyte colony stimulating factor , in either bulk or single cell serum free cultures , markedly reduces their ability subsequently to generate colony forming cells ( CFCs ) in 6 week stromal cell containing long term cultures without affecting their viability , mitogenic response , or short term ability to produce CFCs . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The role of granulocyte macrophage colony stimulating factor ( GM CSF ) and Flt 3 ligand in the in vivo development of Langerhans cells ( LC ) was assessed , considering both the steady state levels of LC in the epidermis and the rate of LC recovery after depletion following lipopolysaccharide ( LPS ) treatment . ^^^ LC levels were compared in beta common chain receptor null ( beta c ( / ) ) mice that fail to respond to GM CSF interleukin 5 ( IL 5 ) , in GM CSF transgenic mice with elevated GM CSF levels , and in mice given daily injections of Flt 3 ligand . ^^^ In the steady state , LC levels were increased in GM CSF transgenic mice and present at reduced levels in beta c ( / ) mice but unchanged in Flt 3 ligand injected mice . ^^^ This recovery was significantly delayed in beta c ( / ) mice and unchanged in Flt 3 ligand injected mice . ^^^ These results suggest that GM CSF ( but not Flt 3 ligand ) enhances recruitment / maturation of LC even though GM CSF is not essential for their formation . . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Cell surface trafficking and release of flt 3 ligand from T lymphocytes is induced by common cytokine receptor gamma chain signaling and inhibited by cyclosporin A . ^^^ The flt 3 ligand ( FL ) is a growth and differentiation factor for primitive hematopoietic precursors , dendritic cells , and natural killer cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Pretreatment with recombinant Flt 3 ligand partially protects against progressive cutaneous leishmaniasis in susceptible BALB / c mice . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
IL 11 and the Flt 3 ligand possessed T lineage promotional effects similar to IL 6 and SCF respectively . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Using a combination of FLT 3 ligand ( FL ) , Stem Cell Factor ( SCF ) , Thrombopoietin ( TPO ) and Interleukin 6 ( IL 6 ) , we cultured cord blood ( CB ) CD34+ cells for up to 12 weeks and transplanted their progeny into sublethally irradiated NOD / SCID mice . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
These cells were CD 43 ( pos ) CD 24 ( pos ) on isolation and proliferated in response to the cytokine combination of steel factor , IL 7 , and Flt 3 ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
CD34+ hematopoietic cells were cultured in serum free medium with megakaryocyte growth and development factor ( MGDF ) alone for supporting the survival of primitive progenitors or with MGDF , c kit ligand ( KL ) and flt 3 ligand ( FL ) for inducing proliferation of primitive progenitors . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Expression of recombinant human soluble Flt 3 ligand and its in vitro effects on malignant hematopoietic cells ] . ^^^ OBJECTIVE : To express human soluble Flt 3 ligand in a yeast expression system . ^^^ Pichia pastoris , and investigate the effects of recombinant human soluble Flt 3 ligand ( rhFL ) on the proliferation of malignant hematopoietic cells ( MHC ) and the effect of dexamethasone ( DXM ) on the expression of Flt 3 receptor ( Flt3R ) and the rhFL induced proliferation of those cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Leukemic dendritic cells generated in the presence of FLT 3 ligand have the capacity to stimulate an autologous leukemia specific cytotoxic T cell response from patients with acute myeloid leukemia . ^^^ In this study we differentiated peripheral blood AML cells in vitro into functional dendritic cells ( DCs ) , as demonstrated by cell morphology , immunophenotype and functional activity , in the presence of GM CSF , IL 4 , TNF alpha and FLT 3 ligand . ^^^ The use of FLT 3 ligand in our system resulted in a significantly higher number of leukemic DCs as compared to cultures from which FLT 3 ligand was omitted which is obviously advantageous if large numbers of specific CTLs are to be generated in the shortest possible time . . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
STUDY DESIGN AND METHODS : Cord blood CD34+ cells were cultured in Iscove ' s modified Dulbecco medium supplemented with 10 percent fetal calf serum ( FCS ) and IL 6 , SCF , megakaryocyte growth and development factor , and Flt 3 ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Cultures of CD34+ cells with the combination of FLT 3 ligand + GM CSF + IL 4 + TNF alpha yielded 24 . 28 % + / 2 . 14 % CD1a+ cells , while the control cultures did not . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Granulocyte macrophage colony stimulating factor ( GM CSF ) is a primary growth factor for DCs in vitro , but recently it was recognized that other factors including flt 3 ligand ( FL ) and G CSF expand various DC subsets in vivo . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Il 12 antagonism enhances apoptotic death of T cells within hepatic allografts from Flt 3 ligand treated donors and promotes graft acceptance . ^^^ By contrast , livers from donors treated with Flt 3 ligand , which dramatically increases hepatic interstitial dendritic cells , are rejected acutely ( median survival time 5 days ) . ^^^ Therefore , C3H ( H 2 ( k ) ) recipients of liver grafts from Flt 3 ligand treated B 10 donors were given neutralizing anti IL 12 mAb ( 200 or 500 microg ) on days 0 and 2 after transplant . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
More importantly , the regulatory proteins VEGF , HGF , FGF 2 , KL , FLT 3 ligand , TPO , IL 16 , IGF 1 , transforming growth factor beta 1 ( TGF beta 1 ) , TGF beta 2 , RANTES , MIP 1alpha , MIP 1beta , IL 8 , and PF 4 were identified in media conditioned by these cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand ( FL ) treatment of murine donors does not modify graft versus host disease ( GVHD ) but FL treatment of recipients post bone marrow transplantation accelerates GVHD lethality . ^^^ Flt 3 ligand ( FL ) is a hematopoietic cytokine that has been shown to facilitate the expansion of dendritic cells ( DCs ) and the generation of antitumor immune responses . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Neutralization experiments revealed differential contributions of Flt 3 ligand and thrombopoietin to the support of total cell and CFC expansion by Sl / M2 and Sl / Sl stromal feeders . . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Treatment of mice with Flt 3 ligand resulted in an increased number of DC in the buccal mucosa and enhanced the frequency of IFN gamma producing NP specific effectors and the NP specific CTL response generated after buccal immunization with NP . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Whereas Flt 3 ligand ( FL ) , stem cell factor ( SCF ) , and thrombopoietin ( TPO ) are considered indispensable , the role of interleukin 3 ( IL 3 ) is still controversial : it has been reported either to support or abrogate the reconstituting ability of stem cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Altered peptide ligand vaccination with Flt 3 ligand expanded dendritic cells for tumor immunotherapy . ^^^ Although DCs are rare in the blood , we found that treatment of advanced cancer patients with Flt 3 ligand , a hematopoietic growth factor , expanded DCs 20 fold in vivo . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Role of Flt 3 ligand . ^^^ An alternative approach is to directly expand and / or activate DC in vivo using the cytokine Flt 3 Ligand ( FL ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
OBJECTIVE : We evaluated the frequency of hematopoietic progenitor cells ( HPC ) in CD 34 ( + ) CLA ( + ) ( cutaneous lymphocyte associated antigen ) and CD 34 ( + ) CLA ( ) cord blood cells , and followed cellular growth and HPC production during cultures in Flt 3 ligand , thrombopoietin , and stem cell factor ( FTS ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
To assess the hematopoietic defect involved in PNH , CD34+ CD59+ ( normal phenotype hematopoietic stem / progenitor ) and CD34+ CD 59 ( PNH phenotype ) cells from PNH patients ( n = 16 ) and CD34+ CD59+ cells from healthy volunteers ( n = 10 ) were sorted as single cells into 96 well flat bottom culture plates containing culture medium supplemented with stem cell factor , interleukin ( IL ) 3 , erythropoietin , granulocyte macrophage colony stimulating factor ( GM CSF ) , G CSF , IL 6 , thrombopoietin , and Flt 3 ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In contrast , Flt 3 ligand ( FL ) is a positive effector of CD 34 ( + ) CD 38 ( / low ) cell proliferation . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Human peripheral blood ( PB ) CD34+ cells ( > or = 90 % purity ) were cultured for up to 4 days in serum free culture medium supplemented with thrombopoietin ( TPO ) , stem cell factor ( SCF ) , Flt 3 ligand ( Flt 3L ) , with or without PIXY 321 ( IL 3 / GM CSF fusion protein ) and human serum . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand ( FL ) is a cytokine that promotes the survival , proliferation , and differentiation of hematopoietic progenitors in synergy with other growth factors , such as stem cell factor . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Initial in vitro studies with rhesus CD34+ cells indicated that after 4 days of stimulatory culture in stem cell factor ( SCF ) , megakaryocyte growth and development factor ( MDGF ) , and flt 3 ligand ( FLT ) , transfer of the cells to SCF alone on retronectin ( FN ) support resulted in decreased active cycling and a halt to proliferation , without a loss of viability or induction of apoptosis . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
FLT 3 ligand can substitute for macrophage colony stimulating factor in support of osteoclast differentiation and function . ^^^ It was found that when nonadherent bone marrow cells were incubated in RANKL with Flt 3 ligand ( FL ) without exogenous M CSF , tartrate resistance acid phosphatase ( TRAP ) positive cells were formed , and bone resorption occurred . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In previous studies , we identified a cytokine cocktail including thrombopoietin , Flt 3 ligand , interleukin ( IL ) 6 and IL 11 in serum free medium , suitable to induce significant and sustained ex vivo expansion of primitive hematopoietic stem cells ( HSCs ) from cord blood ( CB ) for up to 10 weeks . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand : a novel cytokine prevents allergic asthma in a mouse model . ^^^ Flt 3 ligand ( FL ) , a recently described growth factor affecting early hematopoietic progenitor cells , can also support the expansion of dendritic cells secreting IL 12 . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
CD34+ cells were cultured in 10 VIVO 10 supplemented with thrombopoietin , stem cell factor , and Flt 3 ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
We examined the functional differences between G ( 0 ) and G ( 1 ) cord blood CD34+ cells for up to 24 weeks in serum free suspension culture , containing Flt 3 ligand , thrombopoietin and stem cell factor . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Cell cycle activation of peripheral blood stem and progenitor cells expanded ex vivo with SCF , FLT 3 ligand , TPO , and IL 3 results in accelerated granulocyte recovery in a baboon model of autologous transplantation but G0 / G1 and S / G2 / M graft cell content does not correlate with transplantability . ^^^ We showed in a nonhuman primate model of autologous peripheral blood stem and progenitor cell transplantation that cell cycle activation of CD 34 ( + ) cells in the presence of stem cell factor + FLT 3 ligand + thrombopoietin + interleukin 3 ( six days of culture ) which induced G 1 and S / G2 / M cell amplification ( G 0 : 6 . 1 % + / 2 . 8 % ; G0 / G1 : 64 . 2 % + / 7 . 2 % ; S / G2 / M : 30 . 4 % + / 7 . 3 % respectively of expanded CD 34 ( + ) cells on average ) resulted in the acceleration of short term granulocyte recovery . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Coupling antigen to CTB or administration of Flt 3 ligand enhances oral tolerance . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The combination of thrombopoietin , Flt 3 ligand , and stem cell factor upregulated survivin expression in CD 34 ( + ) cells within 24 hours ; survivin expression was cell cycle related and highest during G2 / M , whereas growth factor withdrawal resulted in decreased survivin expression . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The results indicate that a combination of flt 3 ligand , Steel factor and interleukin 11 or hyper interleukin 6 provide a level of mitogenic stimulation similar to that existing in vivo after a myeloablative radiation dose . ^^^ The comparison of the cell cycle distribution obtained for different cultures of human bone marrow CD 34 ( + ) ( 45RA / 71 ) ( ) cells demonstrates that the addition of flt 3 ligand in these cultures decreases apoptosis significantly but does not reduce quiescence . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Expression of human flt 3 ligand and thrombopoietin genes in a bone marrow stromal cell line by internal ribosome entry site ( IRES ) sequence ] . ^^^ OBJECTIVE : To explore the feasibility of retroviral mediated Flt 3 ligand ( FL ) and thrombopoietin ( Tpo ) genes transferred into and expressed in a bone marrow stromal cell line HFCL by internal ribosome entry site ( IRES ) sequence . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Localization of a FLT 3 ligand isoform in human tissues ] . ^^^ OBJECTIVE : To explore the localization of a human FLT 3 ligand isoform deleted 139 bp of extracellular region . ^^^ METHODS : Localization of the FLT 3 ligand isoform in human tissues was studied by using PCR and RT PCR . ^^^ RESULTS : FLT 3 ligand was expressed in liver , fetal liver , bone marrow , muscle , kidney and peripheral blood cells and in four leukemia cell lines , but not in brain , while the FLT 3 ligand isoform only in fetal liver and bone marrow . ^^^ CONCLUSION : The FLT 3 ligand isoform was expressed in hematopoietic cells and might be involved in hematopoiesis regulation . . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Among various combinations , the combination of c kit ligand , flt 3 ligand , c mpl ligand ( TPO ) , and interleukin ( IL ) 4 most potently generated the number of CD1a+CD14 DCs in cultures containing granulocyte macrophage colony stimulating factor ( GM CSF ) and tumor necrosis factor alpha ( TNF alpha ) . ^^^ These findings demonstrated that the combination of c kit ligand plus flt 3 ligand plus TPO with GM CSF plus TNF alpha , followed by IL 4 , is useful for ex vivo generation of human DCs from mobilized CD34+ peripheral blood progenitors . . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The ability to modify these antigen specific immune responses was investigated by coinjection of DNA plasmids encoding cytokine and / or hematopoietic growth factors , interleukin 2 ( IL 2 ) , IL 12 , IL 15 , Flt 3 ligand ( FL ) , and granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
This report provides baseline data for studies that will show whether other cytokines such as granulocyte macrophage colony stimulating factor ( GM CSF ) and / or Flt 3 Ligand can immunomodulate allotransfusates in vivo to improve the graft vs . leukemia ( GVL ) effect after allogeneic stem cell transplantation , while lowering the incidence and severity of graft vs . host disease ( GVHD ) . . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Effects of dexamethasone on Flt 3 receptor expression and proliferation induced by recombinant human Flt 3 ligand in malignant hematopoietic cells . ^^^ AIM : To investigate the effect of dexamethasone ( DXM ) on the expression of Flt 3 receptor and the proliferation mediated by recombinant human Flt 3 ligand ( rhFL ) in leukemia cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
METHODS : G CSF mobilized peripheral CD 34 ( + ) cells were cultured for 4 days with stem cell factor , flt 3 ligand , and thrombopoietin . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Apart from granulocyte macrophage CSF ( GM CSF ) , there have as yet been no reports of lithium salts inducing early activating factors for the megakaryocytic lineage , such as IL 3 , IL 11 , stem cell factor and flt 3 ligand , or maturation factors , such as thrombopoietin ( TPO ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
It could be increased in 5 7 days when the cells were cultured in vitro , especially in the presence of stem cell factor , interleukin 3 ( IL 3 ) , IL 6 and Flt 3 Ligand together ; the positive rate reached 48 % . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
STUDY DESIGN AND METHODS : Enriched CB CD34+ cells were cultured in four media ( Iscove ' s modified Dulbecco ' s medium with FCS , Gibco ; 10 Vivo 10 , BioWhittaker ; QBSF 60 , Quality Biological ; and StemSpan SFEM , Stem Cell Technologies ) with four cytokine combinations ( thrombopoietin [ TPO ] , SCF , Flt 3 ligand [ FL ] with and without G CSF , and / or IL 6 ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The growth kinetics of two populations of muscle stem cells were characterized in culture medium supplemented with epidermal growth factor ( EGF ) , fibroblast growth factor 2 ( FGF 2 ) , insulin like growth factor 1 ( IGF 1 ) , FLT 3 ligand , hepatocyte growth factor , or stem cell factor ( SCF ) . ^^^ Both MDSC and MC 13 growth were inhibited in the presence of FLT 3 ligand by increasing the length of the cell cycle . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Stem Cell Factor ( SCF ) + IL 6 and FLT 3 ligand , which have no effect on colony formation by themselves , were used to enhance the effects of IL 3 and GM CSF , respectively . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Ex vivo expansion of stem and progenitor cells in co culture of mobilized peripheral blood CD34+ cells on human endothelium transfected with adenovectors expressing thrombopoietin , c kit ligand , and Flt 3 ligand . ^^^ Immortalized human bone marrow endothelial cells ( BMEC ) transfected with an expression vector carrying cDNA encoding the human telomerase reverse transcriptase ( hTERT ) and human umbilical vein endothelial cells ( HUVEC ) were transfected with combinations of adenovectors expressing murine c kit ligand ( KL ) , human thrombopoietin ( TPO ) , human Flt 3 ligand ( FL ) , and human granulocyte macrophage colony stimulating factor ( GM CSF ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In this study we combined a dendritic cell growth factor , Flt 3 ligand , with a dendritic cell activator , immunostimulatory DNA , and a tumor antigen to activate and load dendritic cells in vivo . ^^^ Following treatment of mice with Flt 3 ligand , coadministration of immunostimulatory DNA and antigen induced potent antitumor immunity , resulting in both tumor prevention and regression of existing tumors . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand therapy for recipients of allogeneic bone marrow transplants expands host CD 8 alpha ( + ) dendritic cells and reduces experimental acute graft versus host disease . ^^^ Flt 3 ligand ( FL ) derived DC function was tested as a stimulator of allogeneic lymphocytes in vitro and in vivo . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Comparison of the functional properties of murine dendritic cells generated in vivo with Flt 3 ligand , GM CSF and Flt 3 ligand plus GM SCF . ^^^ Flt 3 ligand ( FL ) and granulocyte macrophage colony stimulating factor ( GM CSF ) are important growth factors for dendritic cells ( DC ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Human CD34+ cells from CB or PBSC were cultured in serum free medium , with or without hematopoietic growth factors ( FKT : FLT 3 ligand [ FL ] , KIT ligand [ KL ] , and thrombopoietin [ TPO ] ) , and with or without soluble Fas ligand ( sFasL ) or agonistic anti Fas antibody . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In this study , we investigated the effects of the cytokines flt 3 ligand , stem cell factor , and thrombopoietin on expansion of CD34+ and CD34+ / CD38 cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Therapeutic effect of colon tumor cells expressing FLT 3 ligand plus systemic IL 2 in mice with syngeneic colon cancer . ^^^ Flt 3 ligand ( FL ) and interleukin 2 ( IL 2 ) have been shown to enhance individually the antitumor response against several cancers . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The hematopoietic potential of these CD34+ / CD105+ cells appears to be sustained by a combination of hematopoietic and non hematopoietic cytokines , which comprises Flt 3 ligand , erythropoietin , interleukin 15 and vascular endothelial growth factor . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Enhanced antitumoral effectiveness of idiotype vaccination induced by the administration of Flt 3 ligand combined with interleukin 2 against a murine myeloma . ^^^ In an attempt to improve the therapeutic effectiveness of Id vaccination , we added Flt 3 ligand ( Flt 3 L ) and interleukin 2 ( IL 2 ) to the protocol . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Expansion of haemopoietic stem cells from placental blood has been obtained with a combination of flt 3 ligand ( FL ) , thrombopoietin ( TPO ) , kit ligand ( KL ) with or without interleukin 6 ( IL 6 ) in serum replete medium . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In contrast , rhSCGF alpha suppressed stem cell factor ( SCF ) stimulated erythroid bursts , indicating some competitive interaction between SCGF and SCF . rhSCGF alpha was synergistic with interleukin 3 and the flt 3 ligand to enhance GM colony growth , but not synergistic with those inducing ex vivo expansion of GM progenitor cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Effects of administration of progenipoietin 1 , Flt 3 ligand , granulocyte colony stimulating factor , and pegylated granulocyte macrophage colony stimulating factor on dendritic cell subsets in mice . ^^^ We studied the effects of administration of several cytokines , including progenipoietin 1 ( ProGP 1 ) , Flt 3 ligand ( FL ) , granulocyte colony stimulating factor ( G CSF ) , and granulocyte macrophage colony stimulating factor in a pegylated form ( pGM CSF ) , on dendritic cell ( DC ) populations in mouse spleen . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
CIP 29 mRNA expression is cytokine regulated in hematopoietic cells , being up regulated by Epo in UT7 / Epo cells , and by thrombopoietin ( Tpo ) , FLT 3 ligand ( FL ) and stem cell factor ( SCF ) in primary human CD 34 ( + ) cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Antitumor effects of Flt 3 ligand in transplanted murine tumor models . ^^^ Administration of Flt 3 ligand ( FL ) to mice causes dendritic and natural killer cells to increase but certain solid tumors to regress . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand as a vaccine adjuvant in association with HER 2 / neu peptide based vaccines in patients with HER 2 / neu overexpressing cancers . ^^^ Systemic treatment with Flt 3 ligand ( FL ) results in a marked increase of DCs in tissues such as spleen and lymph nodes in mice and in the peripheral blood and skin of humans . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In the present study , the effects of murine bone marrow endothelial cell conditioned medium ( ECM ) combined with flt 3 ligand ( FL ) or / and thrombopoietin ( TPO ) on the proliferation of HPP CFC and CFU GM were investigated . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Recombinant human Flt 3 ligand exerts both direct and indirect effects on hematopoiesis . ^^^ OBJECTIVE : To investigate the direct effects of the Flt 3 ligand ( FL ) on hematopoiesis , such as the stimulation of the formation of hematopoietic colonies and the proliferation of dendritic cells , as well as the indirect stimulation of hematopoiesis , especially via the proliferation of endothelial cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Intramuscular co injection of naked DNA encoding HBV core antigen and Flt 3 ligand suppresses anti HBc antibody response . ^^^ Flt 3 ligand , a recently described growth factor affecting early hematopoietic progenitor cells , can also support the expansion of dendritic cells secreting IL 12 . ^^^ Here , we studied the effect of in situ delivery of Flt 3 ligand plasmid ( FL ) on the antibody response induced by DNA vaccine encoding wild type hepatitis B virus core antigen ( HBc / w ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
To determine the ability of these factors to alter the transplantability of human HSCs proliferating in vitro , lin ( ) cord blood cells were first cultured for 96 hours in serum free medium containing Flt 3 ligand , Steel factor , interleukin 3 , interleukin 6 , and granulocyte colony stimulating factor . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Cell surface immunophenotyping of spleen derived fibroblasts revealed a low and consistent expression of IL 15 , Flt 3 ligand , and c kit ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Strain dependent variation existed among C57BL / 6 ( B 6 ) , DBA / 2 , and BXD recombinant inbred mice in the responsiveness of primitive progenitor cells to the early acting cytokines kit ligand , flt 3 ligand , and thrombopoietin . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Expression of human FLT 3 ligand gene in human bone marrow stromal cells ] . ^^^ OBJECTIVE : To construct a retroviral mediated vector of FLT 3 ligand ( FL ) and express it in human bone marrow stromal cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Our data demonstrated that among various cytokine combinations of thrombopoietin ( TPO ) , interleukin 1 beta ( IL 1beta ) , IL 3 , IL 6 and Flt 3 ligand ( Flt 3L ) , TPO + IL 6 + Flt 3L was most efficient in promoting the expansion of CD34+ cells , CD34+CD38 cells , mixed lineage colony forming units ( CFU GEMM ) and long term culture initiating cells ( LTC IC ) by 21 . 7 + / 5 . 00 , 103 + / 27 . 9 , 10 . 7 + / 7 . 94 and 6 . 52 + / 1 . 51 fold , respectively , after 12 14 d of culture . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
MATERIAL AND METHODS : To perform our experiments we used a stroma free cell culture system in which the combination of FLT 3 ligand ( FL ) , stem cell factor ( SCF ) and thrombopoietin ( TPO ) induces massive expansion and proliferation of cord blood HP . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Differential response to stem cell factor and Flt 3 ligand by the FAB subtype in acute myeloid leukemia clonogenic cells . ^^^ Proliferative response of blast clonogenic cells to various hematopoietic growth factors ( HGF ) , including stem cell factor ( SCF ) and flt 3 ligand ( FL ) was investigated in 100 patients with acute myeloid leukemia ( AML ) and chronic myelogenous leukemia ( CML ) in myeloid crisis ( MC ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Growth without differentiation was sustained only in the presence of early acting HGF , namely flt 3 ligand , while early and unilineage HGF gave rise to all types of hemopoietic colonies . . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand ( FL ) drives differentiation of rat bone marrow derived dendritic cells expressing OX 62 and / or CD 161 ( NKR P 1 ) . ^^^ Here , large quantities of bone marrow derived rat DC were generated when Flt 3 ligand ( FL ) was used as an adjunct to granulocyte macrophage colony stimulating factor ( GM CSF ) and interleukin 4 ( IL 4 ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
METHODS : Bone marrow leukocytes were incubated with antigen encoding lentiviral constructs and cultured with GM CSF , IL 4 , and Flt 3 ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In animal models and to a lesser extent in humans , other cytokines such as thrombopoietin and Flt 3 ligand or a number of engineered small molecules with single or dual agonist activity for cytokine receptors ( IL 3 , Flt 3L , TPO , G CSF ) , have also been found to be promising mobilising agents . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Recipient mice were treated for 1 or 7 days with growth factors ( stem cell factor [ SCF ] plus interleukin 11 [ IL 11 ] , SCF plus Flt 3 ligand [ FL ] , or granulocyte colony stimulating factor [ G CSF ] ) prior to low dose TBI ( 4 Gy ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
For example , Flt 3 ligand ( FL ) , in conjuction with IL 6 ( in the absence of GM CSF ) , has been reported to be able to induce DC differentiation from BM cells in a murine system . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Combinations of thrombopoietin and cytokines that act on megakaryocyte development ( stem cell factor , IL 3 , IL 6 , IL 11 , flt 3 ligand ( now known as FLT3LG ) , erythropoietin , GM CSF and G CSF were evaluated for their ability to enhance clonal growth in vitro of 10 irradiated CD 34 ( + ) megakaryocytic progenitor cells ( CFU megakaryocytes ) purified from normal human peripheral blood . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand expands dendritic cell numbers in normal and malignant murine prostate . ^^^ Recombinant human Flt 3 ligand ( rhFL ) expands the number of dendritic cells in lymphoid and non lymphoid tissues of mice . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Gene transfer of murine Flt 3 ligand mediated by adenoviral vector efficiently induces growth inhibition of murine liver cancer ] . ^^^ OBJECTIVE : To observe the in vivo therapeutic effects of murine Flt 3 ligand ( mFL ) mediated by recombinant adenoviral vector on murine liver cancer . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
METHOD : The CD ( 34 ) ( + ) CD ( 59 ) ( + ) and CD ( 34 ) ( + ) CD ( 59 ) ( ) cells from PNH patients and CD ( 34 ) ( + ) CD ( 59 ) ( + ) cells from normal volunteers were sorted as each single cell into a well of 96 well culture plates containing culture medium supplemented with SCF , IL 3 , Epo , GM CSF , G CSF , IL 6 , Tpo and Flt 3 ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
METHODS : CD34+ cell preparations purified from CB or PBSC were cultured in serum free medium containing FKT : FLT 3 ligand ( FL ) , KIT ligand ( KL ) , and thrombopoietin ( TPO ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Cord blood CD34+ cells were incubated with supernatants containing retrovirus for 48 h with cytokines such as IL 3 , IL 6 , SCF , Flt 3 ligand ( FL ) , bFGF and IGF 1 . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
METHODS : Human mobilized peripheral blood ( MPB ) CD 34 ( + ) cells were cultured with the cytokines thrombopoietin mimetic peptide ( mTPO ) , flt 3 ligand ( FL ) , and c kit ligand ( KL ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Residual CD34+ cells were successfully expanded in serum free medium in the presence of antiapoptotic cytokine combination ( stem cell factor + FLT 3 ligand + thrombopoietin + interleukin 3 , 50 ng / mL each , i . e . , 4F ) : KCD34+ = x2 . 8 and x13 . 7 ( n = 2 ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
To study a possible role of neutrophils in granulocyte colony stimulating factor ( G CSF ) induced hematopoietic mobilization , we assessed the number of circulating CD34+ cells in healthy allogeneic stem cell donors on days 3 , 4 , and 5 of mobilization for comparison with the number of peripheral blood neutrophils and the plasma levels of IL 8 , Flt 3 ligand ( FL ) , matrix metalloproteinase 9 ( MMP 9 ) , and human neutrophil elastase ( HNE ) . ^^^ No change in plasma levels of IL 8 or Flt 3 ligand was observed , suggesting that these cytokines do not play a role in stem cell mobilization . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Serum Flt 3 ligand variation as a predictive indicator of hematopoietic stem cell mobilization . ^^^ Flt 3 ligand ( FL ) is a good indicator of bone marrow ( BM ) cellularity , having a reciprocal relationship with white blood cell ( WBC ) count in aplastic anemia and chemotherapy induced neutropenia . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Apoptosis may be significantly reduced with a combination of 4 cytokines ( 4F ) : Stem Cell Factor ( SCF ) , FLT 3 Ligand ( FL ) , thrombopoietin ( TPO ) , and interleukin 3 ( IL 3 ) , each at 50 ng 10 mL ( 1 ) ( 15 % survival versus < 3 % untreated cells , 24 h post irradiation at 2 . 5 Gy ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand augmentation of T cell mitogenesis and expansion of type 1 effector / memory T cells . ^^^ Herein we report mechanisms whereby Flt 3 ligand ( FL ) augments steady state T cell activity in addition to the expansion of dendritic cells ( DCs ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand ( FL ) dramatically increases the number of immunostimulatory dendritic cells ( DC ) and their precursors in bone marrow ( BM ) and secondary lymphoid tissues . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Normal donor bone marrow is superior to Flt 3 ligand mobilized bone marrow in prolonging heart allograft survival when combined with anti CD40L ( CD 154 ) . ^^^ Flt 3 ligand ( FL ) administration markedly increases bone marrow ( BM ) stem cells and immature dendritic cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Multiple signals are required for maturation of human dendritic cells mobilized in vivo with Flt 3 ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Sorted NBM CD 34 ( + ) CD 133 ( + ) and CD 34 ( + ) CD 133 ( ) subsets were evaluated for Mk clonogenic capacity before and after in vitro proliferation in serum free liquid culture containing kit ligand , Flt 3 ligand , thrombopoietin , interleukin 3 , and interleukin 6 . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In vivo generation of dendritic cells by intramuscular codelivery of FLT 3 ligand and GM CSF plasmids . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Up regulation of survivin by thrombopoietin ( Tpo ) , Flt 3 ligand ( FL ) , and stem cell factor ( SCF ) occurred in underphosphorylated retinoblastoma protein ( Rb ) ( positive ) , Ki 67 ( negative ) , and cyclin D ( negative ) CD 34 ( + ) cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Recent studies have identified thrombopoietin ( TPO ) , flt 3 ligand ( FL ) , Steel factor ( SF ) , and interleukin 11 ( IL 11 ) as cytokines able to stimulate amplification of the most primitive murine hematopoietic cells in vitro . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
FLT 3 receptors containing ITD mutations ( FLT3 / ITDs ) are constitutively activated in the absence of FLT 3 ligand ( FL ) stimulation leading to the activation of downstream signaling proteins , including ERK and STAT 5 . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Murine stromal cells producing hyper interleukin 6 and Flt 3 ligand support expansion of early human hematopoietic progenitor cells without need of exogenous growth factors . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand and thrombopoietin serum levels during peripheral blood stem cell mobilization with chemotherapy and recombinant human glycosylated granulocyte colony stimulating factor ( rhu G CSF , lenograstim ) and after high dose chemotherapy . ^^^ The purpose of this investigation was to study thrombopoietin ( TPO ) and Flt 3 ligand ( FL ) serum levels in the course of peripheral blood stem cell ( PBSC ) mobilization and high dose chemotherapy ( HDC ) and to correlate the values with stem cell yield and engraftment . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Baseline cortisol , flt 3 ligand , IL 6 , and G CSF levels were similar in runners and controls . ^^^ IL 6 and G CSF increased to higher levels after M compared with HM , whereas cortisol and flt 3 ligand increased similarly postrace . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Key role of flt 3 ligand in regulation of the common lymphoid progenitor but not in maintenance of the hematopoietic stem cell pool . ^^^ In this report we present the first evidence for a nonredundant regulator of this process , in that adult mice deficient in expression of the flt 3 ligand ( FL ) have severely ( 10 fold ) reduced levels of the CLP , accompanied by reductions in the earliest identifiable B and T cell progenitors . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Consistent with the in vivo repopulating deficiency , when Sca 1 ( + ) c kit ( + ) lin ( ) ( KLS ) cells were isolated and stimulated with growth factors in vitro , up to a 13 fold reduced expansion of total nucleated cells was observed in response to cocktails containing interleukin 3 ( IL 3 ) , IL 6 , stem cell factor ( SCF ) , Flt 3 ligand , and thrombopoietin . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In light of our earlier observation that ICSBP ( / ) mice lack CD8alpha ( + ) DCs , we investigated the role of ICSBP in DC development in vitro in the presence of Flt 3 ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Wild type FLT 3 and vector transfected 32D cells were able to differentiate , although the maturation of FLT 3 transfected cells was delayed by FLT 3 ligand ( FL ) stimulation . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
During myeloid / monocytic differentiation induced by incubation with stem cell factor , thrombopoietin , and flt 3 ligand , partially differentiated HSCs emerge , which readily take up these pathogens and also latex beads by macropinocytosis . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
This study investigated early ex vivo expansion kinetics of UCB AC133+ cells ( 2 4 10 10 ( 4 ) / ml ) , mononuclear cells ( MNC , 1 2 10 10 ( 6 ) / ml ) and AC133negative cells ( AC 133 ( neg ) , 2 4 10 10 ( 4 ) / ml ) in stroma free 8 d liquid culture ( fetal bovine serum supplemented Iscove ' s modified Dulbecco ' s medium ( IMDM ) with either ' K36EG ' [ c Kit ligand , interleukin 3 ( IL 3 ) , IL 6 , erythropoietin , granulocyte colony stimulating factor ] or ' TPOFL ' ( thrombopoietin , Flt 3 ligand ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In addition , the compound reverts the antiapoptotic and pro proliferative activity of FLT 3 ligand ( FL ) in FL dependent cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Increasing bioactivity of Flt 3 ligand by fusing two identical soluble domains . ^^^ Flt 3 ligand ( FL ) is a hematopoietic growth factor , initiating its in tracellular signaling cascade by binding to counterpart receptor and driving receptor dimerization . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Pure population of CD 34 ( + ) cells was incubated in a serum free medium supplemented with thrombopoietin , stem cell factor and Flt 3 ligand for 48 h and then transfected with plasmid DNA carrying the enhanced version of green fluorescent protein ( EGFP ) as a reporter gene . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Adjuvant effect of a Flt 3 ligand ( FL ) gene transduced xenogeneic cell line in a murine colon cancer model . ^^^ BACKGROUND : Flt 3 Ligand ( FL ) has been shown to elicit antitumor responses induced by tumor antigen stimulation . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The use of Flt 3 ligand as an adjuvant for hepatitis B vaccination of healthy adults . ^^^ A phase I / II clinical trial was carried out to determine the safety of Flt 3 ligand used as a vaccine adjuvant when administered to healthy human volunteers on two different schedules . ^^^ In the first phase of this study , Flt 3 ligand was administered SQ at a dose of 20 microg / kg ( to a maximum of 1500 microg ) every day ( N=10 ) or every other day ( N=10 ) for 1 week . ^^^ The Flt 3 ligand injection series was followed 1 day later by the first of three vaccinations with the licensed hepatitis B vaccine . ^^^ In the second phase of the trial , 30 volunteers received either Flt 3 ligand or placebo on the alternate day schedule in a randomized , double blind design . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In contrast , we previously reported that Survivin is expressed throughout the cell cycle in normal CD 34 ( + ) hematopoietic stem and progenitor cells stimulated by the combination of Thrombopoietin ( Tpo ) , Stem Cell Factor ( SCF ) and Flt 3 ligand ( FL ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Interestingly , after 72 hours in serum free culture with or without Steel factor ( SF ) , Flt 3 ligand ( FL ) , and interleukin 3 ( IL 3 ) , most G ( 0 ) AML cells entered active cell cycle ( percentage of AML cells remaining in G ( 0 ) at 72 hours , 1 . 2 % to 37 % , and 0 % to 7 . 6 % in cultures without and with growth factors [ GFs ] , respectively ) while G ( 0 ) cells from normal lineage depleted bone marrow remained quiescent in the absence of GF . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
This work was aimed at evaluating the in vivo survival effect of combinations of early acting cytokines whose antiapoptotic activity has been demonstrated in vitro : stem cell factor ( SCF [ S ] ) , FMS like tyrosine kinase 3 ligand ( FLT 3 ligand [ F ] ) , thrombopoietin ( TPO [ T ] ) , interleukin 3 ( IL 3 [ 3 ] ) , and stromal derived factor 1 ( SDF 1 ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Murine plasmacytoid pre dendritic cells generated from Flt 3 ligand supplemented bone marrow cultures are immature APCs . ^^^ In this study , we report that large numbers of bone marrow derived murine CD11c ( + ) B 220 ( + ) pDCs can be generated with Flt 3 ligand ( FL ) as the sole exogenous differentiation / growth factor and that pDC generation is regulated in vivo by FL because FL deficient mice showed a major reduction in splenic pDC numbers . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
CD 40 ligandization promotes IL 6 and Flt 3 ligand production of bone marrow stromal cells ] . ^^^ OBJECTIVE : To analyze the expression of the CD ( 40 ) and its ligand ( CD ( 40 ) L ) on bone marrow stromal cells ( BMSC ) , and investigate interleukin 3 ( IL 3 ) , IL 6 , Flt 3 ligand ( FL ) and stem cell factor ( SCF ) production of BMSC after stimulated with CD ( 40 ) agonistic monoclonal antibody ( 5C11 ) and its role in the regulation of hematopoiesis . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand : a potent dendritic cell stimulator and novel antitumor agent . ^^^ Flt 3 ligand ( FL ) is a growth factor that was first discovered by its ability to stimulate the proliferation of hematopoietic progenitor cells of both lymphoid and myeloid origin . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In the present study , long term cultures of the CD 56 ( ) / CD34 ( ) myeloid like adherent cell fraction ( ACF ) from umbilical cord blood ( UCB ) , characterized by the expression of CD 14 ( + ) as well as other myeloid markers , were set up with flt 3 ligand ( FL ) and interleukin 15 ( IL 15 ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
We assessed 8 wk engraftable stem cells over a period spanning at least one cell cycle for cytokine ( FLT 3 ligand , thrombopoietin [ TPO ] , steel factor ) activated marrow stem cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The cytokines included the recombinant human megakaryocyte growth and development ( 100 ng / ml ) , interleukin 3 ( 10 ng / ml ) , stem cell factor ( 100 ng / ml ) , flt 3 ligand ( 50 ng / ml ) and interleukin 11 ( 200 ng / ml ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
We investigated the role of three parameters : A ) the culture medium ( serum free versus serum dependent ) ; B ) the initial cell population ( Ficoll separated mononucleated cells versus CD 34 ( + ) selected cells ) , and C ) the low concentration of recombinant cytokines , flt 3 ligand , and thrombopoietin in association with a basic cocktail of stem cell factor , interleukin ( IL ) 6 , IL 3 , GM CSF , and erythropoietin . ^^^ Cytokine combinations , in particular the use of flt 3 ligand , stimulated expansion of almost all the cellular subsets , reaching a statistical significance for total nuclear cells and CFU GM . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Cells were cultured with stem cell factor ( SCF ) , thrombopoietin ( TPO ) , Flt 3 ligand ( Flt 3 ) , and G CSF . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Although we observed the constitutive phosphorylation of STAT 5 molecules in FLT 3 mutant cells , FLT 3 ligand ( FL ) did not induce STAT 5 phosphorylation in FLT 3 wild type cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Antitumor effect of secreted Flt 3 ligand can act at distant tumor sites in a murine model of head and neck cancer . ^^^ The Flt 3 ligand ( Flt 3 L ) manifests antitumor activity , presumably due to its capacity to recruit dendritic cells and cause their proliferation . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
We replaced granulocyte macrophage colony stimulating factor with macrophage colony stimulating factor from a serum free culture containing granulocyte macrophage colony stimulating factor , stem cell factor , Flt 3 ligand , tumor necrosis factor alpha , and transforming growth factor beta 1 . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
CTS administration in vivo increased mRNA expression of Flt 3 ligand , one of NK generating cytokines , in bone marrow cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Long term maintenance of gp 120 specific immune responses by genetic vaccination with the HIV 1 envelope genes linked to the gene encoding Flt 3 ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Cells are expanded in media supplemented with fetal bovine serum , horse serum , PIXY 321 , flt 3 ligand , and erythropoietin . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand treated neonatal mice have increased innate immunity against intracellular pathogens and efficiently control virus infections . ^^^ Flt 3 ligand ( FL ) , a hematopoetic growth factor , increases the number of dendritic cells ( DCs ) , B cells , and natural killer cells in adult mice but the effect in neonates was unknown . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Conditional expression of murine Flt 3 ligand leads to expansion of multiple dendritic cell subsets in peripheral blood and tissues of transgenic mice . ^^^ We used the tetracycline inducible system to conditionally express murine Flt 3 ligand ( FL ) , a potent hemopoietic growth factor that promotes the differentiation and mobilization of DC . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Proliferation of human progenitor cells in a long term culture system is more efficiently sustained by the addition of Flt 3 ligand or megakaryocyte growth and development factor than by Kit ligand . ^^^ INTRODUCTION : We compared the effects of the early acting growth factors ( GF ) , Flt 3 ligand ( FL ) , c Kit ligand ( KL ) , and leukemia inhibitory factor ( LIF ) , and the late acting GF , granulocyte colony stimulating factor ( G CSF ) and megakaryocyte growth and development factor ( MGDF ) , added alone in human long term marrow culture ( LTMC ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In this study , UCB CD 34 ( + ) cells were cultured for 5 weeks in a stroma free liquid culture system using thrombopoietin , flt 3 ligand , and granulocyte colony stimulating factor . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
After 1 week long in vivo treatment with various combinations of interleukin ( IL ) 15 , flt 3 ligand , stem cell factor , IL 2 , IL 12 , and megakaryocyte growth and differentiation factor , CD56+CD3 cells were detected in bone marrow ( BM ) , spleen , and peripheral blood ( PB ) , comprising 5 % to 15 % of human CD45+ cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
When cultivated in flt 3 ligand , stem cell factor and interleukin 7 ( IL 7 ) in a stroma free culture system MLL ENL transduced as well as control cells showed a wave of B lymphopoiesis . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The production of ProGP 2 on a small and large scale using either anti ( Flt 3 ligand ) antibody affinity chromatography , or a combination of ( NH 4 ) 2SO4 fractionation , anion exchange chromatography , hydrophobic interaction chromatography and preparative reverse phase chromatography is described . ^^^ When the yield of ProGP 2 decreased , the combination of three conventional chromatography steps was required to meet protein purity similar to that achieved by the anti ( Flt 3 ligand ) chromatography method . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Pilot study of an HLA A 2 peptide vaccine using flt 3 ligand as a systemic vaccine adjuvant . ^^^ A pilot vaccine study was conducted to test the safety and immunological efficacy of four monthly immunizations of an MHC class 1 peptide vaccine , the E 75 HLA A 2 epitope from HER 2 / neu , using flt 3 ligand as a systemic vaccine adjuvant . ^^^ Twenty HLA A 2 expressing subjects with advanced stage prostate cancer were randomly assigned to one of four immunization or treatment schedules : ( a ) Flt 3 ligand ( 20 microg / kg per day ) administered subcutaneously daily for 14 days on a 28 day cycle , monthly for four months ; ( b ) flt 3 ligand course as above with the E 75 peptide vaccine administered on day 7 of each flt 3 ligand cycle ; ( c ) flt 3 ligand course as above with the E 75 peptide vaccine administered on day 14 of each flt 3 ligand cycle ; or ( d ) E 75 peptide admixed with granulocyte macrophage colony stimulating factor and administered intradermally once every 28 days , as has previously been reported . ^^^ Dendritic cells were markedly increased in the peripheral blood of subjects receiving flt 3 ligand with each repetitive cycle , but augmentation of antigen presenting cells within the dermis was not observed . ^^^ Apart from a single subject , no significant peptide specific T cell responses were detected by ELIspot , whereas delayed type hypersensitivity responses were detectable in control subjects and in subjects receiving peptide vaccine early in the course of flt 3 ligand administration . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Murine Flt 3 ligand expands distinct dendritic cells with both tolerogenic and immunogenic properties . ^^^ Human Flt 3 ligand can expand dendritic cells ( DC ) and enhance immunogenicity in mice . ^^^ However , little is known about the effects of murine Flt 3 ligand ( mFlt3L ) on mouse DC development and function . ^^^ Adoptive transfer of Ag pulsed mFlt3L splenic DC to naive mice actually caused faster rates of tumor growth and induced minimal CTL compared with control DC . mFlt3L also failed to protect against tumors in which human Flt 3 ligand was protective , but depletion of CD 4 ( + ) T cells restored tumor protection . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
CD 34 progenitor cells were first amplified in vitro with stem cell factor ( SCF ) , Flt 3 ligand ( FL ) , thrombopoietin and IL 6 / soluble IL 6 receptor fusion protein , and cells were then induced to differentiate into DC with IL 4 and GM CSF . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Radioprotective effect of FLT 3 ligand expression regulated by Egr 1 regulated element on radiation injury of SCID mice . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
METHODS : Adult normal bone marrow ( BM ) and mobilized peripheral blood ( MPB ) CD 34 ( + ) cells were cultured in a stroma free liquid culture in the presence of Flt 3 ligand ( FL ) , thrombopoietin ( TPO ) , stem cell factor ( SCF ) , interleukin 6 ( IL 6 ) , or interleukin 3 ( IL 3 ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The cells were cultured in gas permeable Teflon coated bags in serum free medium containing the following cytokines : recombinant human interleukin 3 , recombinant human Flt 3 ligand , recombinant human stem cell factor , and recombinant human thrombopoietin . ^^^ MK precursors can be successfully expanded from CD 34 ( + ) UCB cells in a closed liquid culture system using interleukin 3 , recombinant human Flt 3 ligand , recombinant human stem cell factor , and recombinant human thrombopoietin to a level that should have a clinical impact in the transplantation setting . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
To study further the mechanisms underlying the unique patterns of hematopoietic regeneration after cyclophosphamide , mRNA levels for stem cell factor ( SCF ) , Flt 3 ligand , and macrophage inflammatory factor ( MIP ) 1 alpha cytokines were determined in bone marrow and spleen . ^^^ No significant changes in mRNAs for Flt 3 ligand and MIP 1 alpha have been found . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
STUDY DESIGN AND METHODS : CD34+ cells were analyzed for the expression of CD 38 , CD 133 , and CD 117 and cultured in serum free culture medium with four cytokine combinations : SCF plus thrombopoietin plus flt 3 ligand ( STF ) , STF plus IL 3 , STF plus IL 6 , and STF plus IL 6 plus IL 3 . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Through its ability to promote ex vivo expansion and oncoretroviral transduction of primitive human hematopoietic progenitors , the flt 3 ligand ( FL ) has emerged as a key stimulator of candidate human hematopoietic stem cells ( HSCs ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
DESIGN AND METHODS : To find the best cytokine combination capable of generating large numbers of Mks , baseline CB CD34+ ( bCD34+ ) cells and CD34+ and CD 34 cells , immunoselected after 4 weeks of expansion with thrombopoietin ( TPO ) , stem cell factor ( SCF ) and Flt 3 ligand ( FL ) ( eCD34+ , eCD 34 ) , were further cultured in the presence of different cytokine combinations ( containing interleukin ( IL ) 3 , SCF , TPO and IL 6 ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 mediated signaling in human acute myelogenous leukemia ( AML ) blasts : a functional characterization of Flt 3 ligand effects in AML cell populations with and without genetic Flt 3 abnormalities . ^^^ Flt 3 is activated by binding of its natural Flt 3 ligand ( Flt 3 L ) , but Flt 3 genes with internal tandem duplications ( Flt 3 ITD ) or Asp ( D ) 835 point mutations encode molecules with constitutive activation . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Co administration of Flt 3 ligand counteracts the actions of thrombopoietin in myelosuppressed rhesus monkeys . ^^^ This placebo controlled study evaluated the efficacy of Flt 3 ligand ( FL ) combined with TPO in myelosuppressed rhesus monkeys . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
FLT 3 ligand promotes engraftment of allogeneic hematopoietic stem cells without significant graft versus host disease . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
When vibrissal follicles of adult mice were cultured in the presence of stem cell factor ( SCF ) , interleukin 3 ( IL 3 ) , IL 7 , granulocyte macrophage colony stimulating factor , and Flt 3 ligand , CD45+ / lineage / c kit+ / FcepsilonRI+ cells became detectable on the outgrowing fibroblasts in 10 days and expanded progressively thereafter . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Functional comparison of DCs generated in vivo with Flt 3 ligand or in vitro from blood monocytes : differential regulation of function by specific classes of physiologic stimuli . ^^^ In humans , the major peripheral blood DC ( PBDC ) types , CD1c+ DCs and interleukin 3 receptor positive ( IL 3R+ ) plasmacytoid DCs , are significantly expanded in vivo with the use of Flt 3 ligand ( FL ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Increasing the number of dendritic cells ( DC ) in vivo with flt 3 ligand augmented the expansion and activation of the OVA specific T cells , particularly CD 8 ( + ) T cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
METHODS : Purified human peripheral blood CD34+ cells were cultured in the presence of recombinant human IL 3 , IL 5 , rhGM CSF , SCF , and FLT 3 ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
These cells are distinguished from conventional dendritic cells by their lack of response to Flt 3 ligand and their inability to process antigen . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Preferential ex vivo expansion of megakaryocytes from human cord blood CD34+ enriched cells in the presence of thrombopoietin and limiting amounts of stem cell factor and Flt 3 ligand . ^^^ Our results showed that the use of high concentrations of stem cell factor ( SCF ) and Flt 3 ligand ( FL ) in early CB TPO supplemented cultures was more favorable to monocytic and granulocytic cell expansion . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Dendritic cell subsets in blood and lymphoid tissue of rhesus monkeys and their mobilization with Flt 3 ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Use of matrix metalloproteinase ( MMP ) 9 knockout mice demonstrates that MMP 9 activity is not absolutely required for G CSF or Flt 3 ligand induced hematopoietic progenitor cell mobilization or engraftment . ^^^ To investigate the role of MMP 9 , we compared G CSF and Flt 3 ligand ( Flt 3L ) induced mobilization in wild type ( WT ) and MMP 9 knockout ( KO ) mice . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Effects of a cytokine prestimulation period ( 18 hours ) with interleukin 3 , stem cell factor , Flt 3 ligand , and thrombopoietin were then analyzed in total cells , CFCs , and LTC ICs derived from mPB CD 34 ( + ) cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In WtFLT 3 transfected 32D ( WtFLT 3 32D ) cells , FLT 3 ligand ( FL ) stimulation did not restore the down regulation of Bcl XL but maintained the phosphorylation of Bad . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
NK cells are derived from hematopoietic stem cells , for which stem cell factor or Flt 3 ligand is required in the early stage of differentiation to NK cell progenitors . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Dendritic cells engineered to express the Flt 3 ligand stimulate type 1 immune response , and induce enhanced cytoxic T and natural killer cell cytotoxicities and antitumor immunity . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
METHODS : AC133+ cells selected from fresh cord blood ( CB ) samples were cultured in QBSF 60 serum free media in the presence of Flt 3 ligand + SCF + TPO ( FST ) , with initial addition of IL 3 for up to 2 week . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The expansion effects of mBMEC CM on CFU GM and HPP CFC were enhanced by compounded with flt 3 ligand ( FL ) and thrombopoietin ( TPO ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Plasma Flt 3 ligand concentration correlated with radiation induced bone marrow damage during local fractionated radiotherapy . ^^^ PURPOSE : To determine whether variations in the plasma Flt 3 ligand ( FL ) concentration after radiotherapy ( RT ) may serve as a biomarker for radiation induced bone marrow damage . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Phosphatidylinositol 3 kinase is essential for kit ligand mediated survival , whereas interleukin 3 and flt 3 ligand induce expression of antiapoptotic Bcl 2 family genes . ^^^ Cytokines such as interleukin 3 ( IL 3 ) , kit ligand ( KL ) , and flt 3 ligand ( FL ) promote survival of hematopoietic stem cells and myeloid progenitor cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
CD34+ CD 71 CD 45 cells were cultured in defined serum free media supplemented with 300 ng each of Flt 3 ligand and stem cell factor ( SCF ) , 60 ng of interleukin ( IL ) 3 , and 20 ng each of IL 6 and G CSF . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
FLT 3 ligand causes autocrine signaling in acute myeloid leukemia cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
To address this possibility we added ciprofloxacin and polymyxin B to the drinking water of Balb / c mice mobilized with either interleukin 8 ( IL 8 ) , granulocyte colony stimulating factor ( G CSF ) , or flt 3 ligand ( FL ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
CD 34 ( + ) enriched cell populations from bone marrow were cultured in the presence and absence of human stroma in serum free media containing bFGF , SCF , LIF and Flt 3 ligand for several days . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Single administration of stem cell factor , FLT 3 ligand , megakaryocyte growth and development factor , and interleukin 3 in combination soon after irradiation prevents nonhuman primates from myelosuppression : long term follow up of hematopoiesis . ^^^ The present study investigated the hematopoietic response of cynomolgus monkeys to a single dose of stem cell factor , FLT 3 ligand , megakaryocyte growth and development factor , and interleukin 3 in combination ( 4F , each factor given intravenously at 50 microg / kg ) administered 2 hours after 5 Gy gamma irradiation . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
G CSF / SCF mobilized CD 34 ( + ) rhesus peripheral blood cells were cultured in the presence of SCF , Flt 3 ligand , and MGDF on Retronectin coated flasks . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Orthotopic treatment model of prostate cancer and metastasis in the immunocompetent mouse : efficacy of flt 3 ligand immunotherapy . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Because the origin of tumor cells was not clear and they exhibited a nonlineage specific phenotype , we hypothesized that such tumors likely originated from hematologic myeloid precursor cells and were tentatively assigned the designation `` agranular CD4+ CD56+ hematodermic neoplasms . ' ' In the present study we report 14 cases ( seven already reported and seven additional cases ) of these tumors , and simultaneously we present now a rare population of cells that we have identified in the peripheral blood of healthy volunteers treated with Flt 3 ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
METHODS : CB and APB adherent MNC were cultured in serum free media with GM CSF IL 4 , FLT 3 ligand , tumor growth factor beta ( TGF beta ) , and tumor necrosis factor alpha ( TNF alpha ) for 7 days . ^^^ Neither FLT 3 ligand nor TGF fl enhanced DC expansion . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
OBJECTIVE : Synergy between Flt 3 ligand and G CSF produces a marked expansion of hematopoietic progenitor cells and mobilizes large numbers of stem cells into the peripheral blood . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
NK cells mediate Flt 3 ligand induced protection of dendritic cell precursors in vivo from the inhibition by prostate carcinoma in the murine bone marrow metastasis model . ^^^ Notably , Flt 3 ligand based therapy reversed the inhibitory effects of prostate cancer on dendropoiesis in vivo in wild type and C . ^^^ B 17 SCID ( T and B cell deficient ) mice , but not in SCID beige ( T , B , and NK cell deficient ) animals , suggesting a key role of NK cells in Flt 3 ligand mediated protection of dendropoiesis from tumor induced inhibition in vivo . ^^^ Thus , these data demonstrate that prostate cancer inhibits DC hematopoietic precursors in vivo in the bone marrow and this effect could be abolished by a systemic administration of growth factor Flt 3 ligand . . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
However , when bone marrow ( BM ) progenitors from IL 15Ralpha ( / ) mice were cultured with IL 7 , stem cell factor and flt 3 ligand , followed by IL 15 , they were able to differentiate into functional NK cells , indicating that IL 15Ralpha is not critical for NK cell development . ^^^ Furthermore , the small numbers of NK cells found in IL 15 ( / ) as well as IL 15Ralpha ( / ) but not flt 3 ligand ( / ) mice expressed much lower levels of Ly 49 receptors than those from wild type mice . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Whereas B 1 B cells and Igs are sustained at normal levels throughout life of mice deficient in IL 7 , IL 7Ralpha , common cytokine receptor gamma chain , or flt 3 ligand ( FL ) , we report here that adult mice double deficient in IL 7Ralpha and FL completely lack visible LNs , conventional IgM+ B cells , IgA+ plasma cells , and B 1 cells , and consequently produce no Igs . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Pilot study of Flt 3 ligand comparing intraperitoneal with subcutaneous routes on hematologic and immunologic responses in patients with peritoneal carcinomatosis and mesotheliomas . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Mesenchymal stem cells support expansion of in vitro irradiated CD 34 ( + ) cells in the presence of SCF , FLT 3 ligand , TPO and IL 3 : potential application to autologous cell therapy in accidentally irradiated victims . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Antigen specific IgG elicited in subjects with prostate cancer treated with flt 3 ligand . ^^^ Flt 3 ligand , a growth and differentiation factor for dendritic cells , has shown modest clinical activity in prostate cancer , presumably by eliciting anti cancer immune responses . ^^^ The authors previously reported the results of a clinical trial in which some patients with metastatic prostate cancer treated with flt 3 ligand developed autoimmune hypothyroidism with thyroid specific antibodies . ^^^ Given these findings , the authors hypothesized that anti prostate immune responses might also have been elicited in some subjects treated with flt 3 ligand . ^^^ The authors now report the identification of prostate associated proteins immunologically recognized in 13 prostate cancer patients treated with multiple cycles of flt 3 ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Treatment of hepatocellular carcinoma with adenoviral vector mediated Flt 3 ligand gene therapy . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand treatment reverses endotoxin tolerance related immunoparalysis . ^^^ Given this , we examined whether recovery from immunoparalysis could be accelerated therapeutically with flt 3 ligand , a growth factor that stimulates the differentiation and mobilization of dendritic cells . ^^^ Notably , administration of flt 3 ligand rapidly reverses immunoparalysis in vivo , accelerating and amplifying repopulation of tissues with proinflammatory and immunoregulatory cytokine producing dendritic cells . . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In media supplemented with thrombopoietin , stem cell factor , flt 3 ligand , and granulocyte colony stimulating factor , CD34+ cells , as well as CFU GM , BFU CFU E , CFU GEMM , and CFU MK , increased by 34 . 3 , 138 , 59 . 7 , 38 . 4 , and 86 . 0 fold , respectively . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Functional expression , purification , and characterization of human Flt 3 ligand in the Pichia pastoris system . ^^^ Flt 3 ligand ( FL ) is a potent hematopoietic cytokine that affects the growth and differentiation of hematopoietic progenitor and stem cells both in vivo and in vitro . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Here , we report that the treatment of hES cells during EBs development with a combination of low dose hematopoietic cytokines , including stem cell factor ( SCF ) , Flt 3 ligand , vascular endothelial growth factor ( VEGF ) and human bone marrow stromal cells ( hBMSCs ) , generated cell clusters that contained 8 . 81 % KDR positive hemangioblasts , 9 . 94 % CD 34 positive hematopoietic stem cells and 25 . 7 % CD 45 positive mature hematopoietic cells , and expressed hematopoietic genes such as KDR , stem cell leukemia ( scl ) and runt related transcription factor 1 ( Runx 1 ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Briefly , CD133+ cells , purified from CB using Miltenyi Biotec ' s ( Bergisch Gladbach , Germany ) CliniMACS separation device and the anti CD 133 reagent , were cultured for 3 weeks in a clinical grade closed culture bag system , using the chelator based technology in combination with early acting cytokines ( SCF , thrombopoietin , IL 6 and FLT 3 ligand ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand generated murine plasmacytoid and conventional dendritic cells differ in their capacity to prime naive CD 8 T cells and to generate memory cells in vivo . ^^^ Flt 3 ligand ( FL ) induces the differentiation of conventional DCs ( cDCs ) and plasmacytoid DCs ( PDCs ) from murine bone marrow precursors in vitro . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Abundant gene products novel to microglia were neuromodulin ( GAP 43 ) and Flt 3 ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Bone marrow cells cultured in the presence of granulocyte macrophage colony stimulating factor ( GM CSF ) , interleukin ( IL ) 4 , and Flt 3 ligand ( FL ) produced immature DCs that expressed intermediate levels of major histocompatibility complex ( MHC ) class 2 , low levels of CD 80 and CD 86 molecules and displayed a high capacity of endocytosis . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
However , when RS 4 ; 11 cells were stimulated with FLT 3 ligand , phosphorylation of STAT 5 and GTP 14564 inhibition were observed . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Induction of oral tolerance is enhanced by IL 4 , IL 10 , anti IL 12 , TGF beta , cholera toxin B subunit , Flt 3 ligand , and anti CD 40 ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
When CD36+ erythroid precursor cells , generated from CD34+ cells with stem cell factor , flt 3 ligand , thrombopoietin , interleukin 3 , interleukin 6 , and erythropoietin , were cultured with an HDAC inhibitor FK 228 ( depsipeptide ) at a specified dose in the presence of erythropoietin , their differentiation was inhibited , as determined by the expression of CD 45 and glycophorin A . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Our results demonstrated that CTCE 0214 synergized with thrombopoietin ( TPO ) , stem cell factor ( SCF ) , or flt 3 ligand ( FL ) on the survival of stem and progenitor cells in culture . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
We found that hTERT was incapable of prolonging the replicative capacity of CB cells maintained under serum free conditions in the presence of stem cell factor , Flt 3 ligand , thrombopoietin , and interleukin 3 beyond 4 months ( n=3 ) . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In normal hematopoietic progenitor cells , Flt 3 ligand induces the activation of several downstream signal transduction mediators , including phosphoinositol 3 kinases , Src kinases , mitogen activated protein kinases , and the phosphorylation of several adaptor proteins . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
To explore whether HDAC inhibitors could have a beneficial role in human HSC transplantation , mobilized peripheral blood CD34+ cells were cultured with thrombopoietin mimetic peptide , flt 3 ligand , and c Kit ligand , without or with various HDAC inhibitors . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Flt 3 ligand ( Flt 3L ) is a haematopoietic growth factor that efficiently induces DC expansion in vivo . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Therefore , administration of antiapoptotic cytokine combinations such as stem cell factor , Flt 3 ligand , thrombopoietin , and interleukin 3 ( 4F ) , may be important for multilineage recovery , particularly when these factors are administered early . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Afterwards , a statistical analysis of the two level factorial designs revealed that in the presence of TPO , MK maturation was significantly stimulated by stem cell factor ( SCF ) , interleukin ( IL ) 6 , and IL 9 , whereas Flt 3 ligand ( FL ) had a positive effect only on the expansion of MK progenitors . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Cord blood derived lin ( ) cells were cultured for 8 days in serum free medium supplemented with stem cell factor , Flt 3 ligand , and thrombopoietin . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Primary hMSCs were infected with retrovirus containing Flt 3 ligand and thrombopoietin genes . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Normally , binding of FLT 3 ligand to the FLT 3 receptor leads to phosphorylation of tyrosine residues and activation of the receptor . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
These mutations induce constitutive tyrosine kinase activity in the absence of the natural Flt 3 ligand and confer growth factor independence , increased proliferation , and survival to myeloid precursor cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
A large portion of DCs are derived from hematopoietic progenitors that express FLT 3 receptor ( CD 135 ) , and stimulation of the receptor via FLT 3 ligand either in vivo or in vitro is known to drive expansion and differentiation of these progenitors toward a DC phenotype . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
CD34+CD38 and CD34+CD38+ cells from umbilical cord blood were analyzed before and after culture with thrombopoietin ( TPO ) , FLT 3 ligand ( FL ) and kit ligand ( KL ; or stem cell factor ) in different combinations : TPO + FL + KL , TPO + FL and TPO , at concentrations of 50 ng / mL each . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
We studied the effect of two different cytokine combinations kit ligand ( KL ) , interleukin 3 ( IL 3 ) , and Flt 3 ligand supplemented with thrombopoietin and IL 11 ( combination 1 ) or granulocyte / macrophage colony stimulating factor ( GM CSF ) and G CSF ( combination 2 ) for their ability to affect speed and extent of engraftment using limited numbers ( 5 10 10 ( 4 ) ) of murine bone marrow ( BM ) light density ( LD ) cells or their progeny expanded ex vivo in the presence one or the other cytokine combination for 6 days . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Femoral nucleated bone marrow cell counts and levels of apoptosis were also evaluated and clonogenic assays carried out ; serum levels of FLT 3 ligand ( FL ) were assessed . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Stably expressed in IL 3 dependent Ba / F3 cells , the D324N variant did not confer receptor autophosphorylation , factor independent growth , or increased resistance to apoptotic cell death in response to varying doses of FLT 3 ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Culture supernatants displayed normal stromal derived factor 1 and stem cell factor / kit ligand but increased flt 3 ligand levels . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Kinetics of plasma FLT 3 ligand concentration in hematopoietic stem cell transplanted patients . ^^^ The present study aimed to follow up variations in plasma Flt 3 ligand ( FL ) concentration after hematopoietic stem cell transplantation and to compare the influence of conditioning regimens on variations in FL concentration . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Human CD34+ progenitor cells were cultured onto delta 1 expressing OP 9 stroma in the presence of flt 3 ligand and IL 7 , and this efficiently generated BDCA 2+ CD123+ CD4+ CD11c cells with the characteristic morphology of pDCs , expressing toll like receptor 9 ( TLR 9 ) , pre Talpha mRNAs , and secreting CpG induced IFN alpha . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The importance of early hematopoietic growth factors , such as c kit ligand and flt 3 ligand , and their synergy with IL 15 in the development of human NK cells in the bone marrow has permitted the investigation of novel cytokine combinations for optimizing in vivo expansion of NK cell in the clinic . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
When marrow stem cells are induced to transit cell cycle by in vitro exposure to cytokines , either IL 3 , IL 6 , IL 11 , and steel factor or thrombopoietin , FLT 3 ligand , and steel factor , they progress through cycle in a highly synchronized fashion . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
This method is based on immunoselection of CD34+ cells derived from mobilized peripheral blood of patients with metastatic breast cancer and expansion in the presence of flt 3 ligand , stem cell factor , interleukin 6 , and thrombopoietin . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Expression of 4 FLT 3 JM PMs in interleukin 3 ( IL 3 ) dependent Ba / F3 cells led to factor independent growth , hyperresponsiveness to FLT 3 ligand , and resistance to apoptotic cell death . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Significant reduction of laparotomy associated lung metastases and subcutaneous tumors after perioperative immunomodulation with flt 3 ligand in mice . ^^^ The purpose of this study was to determine the impact on postoperative tumor growth of perioperative upregulation of immune function via fetal liver tyrosine kinase 3 ( Flt 3 ligand ) . ^^^ Each study included four groups : anesthesia control ( AC ) , AC plus Flt 3 ligand ( ACFlt 3 ) , sham laparotomy ( OP ) , and OP plus Flt 3 ligand ( OPFlt 3 ) . ^^^ Flt 3 ligand was administered by daily intraperitoneal injection ( 10 mug / dose ) beginning 5 days preoperatively and continuing for 1 week postoperatively . ^^^ Perioperatively administered Flt 3 ligand was associated with significantly fewer lung metastases and a lower incidence of subcutaneous tumor formation after laparotomy and anesthesia alone . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Increased levels of soluble flt 3 ligand in serum and long term bone marrow culture supernatants in patients with chronic idiopathic neutropenia . ^^^ The levels of serum and long term bone marrow culture supernatant soluble flt 3 ligand ( sFL ) were determined in 54 patients with chronic idiopathic neutropenia ( CIN ) and 16 normal controls . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
After endurance exercise , progenitor counts were unchanged , while plasma Flt 3 ligand and VEGF only increased in the COPD patients . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Subsequently , we tested the reconstituting potential of cells cultured in FGF 1 + 2 with the addition of early acting stimulatory molecules , stem cell factor +interleukin 11 + Flt 3 ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Human Flt 3 ligand from Pichia pastoris inhibits growth of lymphoma and colon adenocarcinoma in mice . ^^^ Potent effects of Flt 3 ligand ( FL ) on the development of the immune system have generated much interest in application of FL in cancer immunotherapy . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
MATERIALS AND METHODS : Human UCB CD 34 ( + ) cells were cultured on MS 5 mouse stroma cells in the presence of stem cell factor ( SCF ) , flt 3 ligand ( FL ) , and thrombopoietin ( TPO ) for 4 to 16 weeks . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
At doses that abrogated megakaryocytopoiesis , HC also inhibited the expansion of CD 34 ( + ) cells stimulated by stem cell factor , interleukin 3 and Flt 3 ligand and also induced apoptosis . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Augmenting the immunogenicity of DNA vaccines : role of plasmid encoded Flt 3 ligand , as a molecular adjuvant in genetic vaccination . ^^^ In this study , we have taken advantage of the unique property of a potent dendritic cell ( DC ) growth factor , Flt 3 ligand ( FL ) , which could act as a vaccine adjuvant . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The expression of early ocular markers ( MITF , Pax 6 , Six 3 , Otx ) in migrated cells and in MNCs isolated from granulocyte colony stimulating factor ( G CSF ) and Flt 3 ligand ( FL ) mobilized and nonmobilized peripheral blood ( PB ) was analyzed by real time RT PCR . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
The long term but not the short term antiviral effect of IFN alpha depends on Flt 3 ligand and pDC . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Furthermore , Akt phosphorylation after stimulation with Flt 3 ligand dependent on D 65476 was not affected by pretreatment with ATRA . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
AIM : To establish a novel coculture system for ex vivo expansion of umbilical cord blood ( UCB ) hematopoietic progenitors using thrombopoietin ( TPO ) / Flt 3 ligand ( FL ) transduced human marrow derived mesenchymal stem cells ( tfhMSCs ) as feeder . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In this report , we investigate the effects of treating ALL cell lines and primary samples with human anti FLT 3 monoclonal antibodies ( mAb ) capable of preventing binding of FLT 3 ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Combinational FLt 3 ligand and granulocyte macrophage colony stimulating factor treatment promotes enhanced tumor infiltration by dendritic cells and antitumor CD 8 ( + ) T cell cross priming but is ineffective as a therapy . ^^^ We have observed that the treatment of BALB / c mice bearing syngeneic CMS 4 sarcomas with the combination of recombinant Flt 3 ligand and recombinant granulocyte macrophage colony stimulating factor ( GM CSF ) for five sequential days is sufficient to optimize the number of tumor infiltrating dendritic cells ( TIDC ) . ^^^ However , despite the significant increase in the number of TIDCs , the therapeutic benefit of Flt 3 ligand and GM CSF treatment is minimal . ^^^ These data suggest that Flt 3 ligand + GM CSF therapy of murine tumors fails at a mechanistic point that is downstream of specific T cell priming by therapy induced TIDCs and the recruitment of these T cells into the tumor microenvironment . ^^^ Based on the enhanced infiltration of tumors by CD 4 ( + ) CD 25 ( + ) TIL in Flt 3 ligand + GM CSF treated mice , this could reflect the dominant influence of regulatory T cells in situ . . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Focusing on Y 589 and Y 599 , we examined Flt 3 ligand ( FL ) mediated responses in wild type Flt 3 ( WT Flt 3 ) , Y589F Flt 3 , and Y599F Flt 3 expressing 32D cells . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
A novel indirect co culture system was established to support ex vivo expansion of hematopoietic progenitors in umbilical cord blood ( UCB ) by using thrombopoietin ( TPO ) / Flt 3 ligand ( FL ) transduced human marrow derived mesenchymal stem cells ( tfhMSCs ) as a feeder . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
As a member of the subclass 3 family of receptor tyrosine kinases , KIT is closely related to the receptors for platelet derived growth factor alpha and beta ( PDGF A and B ) , macrophage colony stimulating factor ( M CSF ) , and FLT 3 ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Sixty female A / J mice were divided into six equal groups designated ( 1 ) anesthesia control ( AC ) , ( 2 ) AC with Flt 3 ligand ( ACFlt 3 ) , ( 3 ) sham laparotomy ( OP ) , ( 4 ) OP with Flt 3 ligand ( OPFlt 3 ) , ( 5 ) OP with vaccine ( OPVac ) , and ( 6 ) OP with Flt 3 ligand and vaccine ( OPFlt3Vac ) . ^^^ Groups 2 , 4 , and 6 received daily intraperitoneal injections of Flt 3 ligand ( 10 microg / dose with carrier ) for 5 days before and 5 days after surgery . ^^^ RESULTS : Regarding laparotomy , Flt 3 ligand ( mean , 1 . 22 metastases ) , whole cell vaccine ( 1 . 12 metastases ) , and the combination of these two agents ( 0 . 1 metastases ) were each effective in significantly decreasing the number of surface lung metastases compared with surgery alone ( 9 . 88 metastases , P < . 05 for all comparisons ) . ^^^ CONCLUSIONS : Perioperative treatment with either Flt 3 ligand or a whole cell tumor vaccine significantly reduced the number of lung metastases after laparotomy . ^^^ The combination of the Flt 3 ligand and the vaccine also decreased the incidence of metastases and was the most effective treatment . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Isolated cells were in the presence of cytokine PBSCs , Flt 3 ligand , interleukin 3 , interleukin 6 , and granulocyte colony stimulating factor . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
We have investigated the influence of different hematopoietic growth factors , including granulocyte colony stimulating factor ( G CSF ) , stem cell factor ( SCF ) , Flt 3 ligand ( Flt 3L ) and thrombopoietin ( TPO ) , on the course of relapsing experimental autoimmune encephalomyelitis , a mouse model of multiple sclerosis . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Use of flt 3 ligand to evaluate residual hematopoiesis after heterogeneous irradiation in mice . ^^^ We evaluated the possibility of using plasma Flt 3 ligand ( FL ) concentration as a biological indicator of bone marrow function after heterogeneous irradiation . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Blood dendritic cells generated with Flt 3 ligand and CD 40 ligand prime CD8+ T cells efficiently in cancer patients . ^^^ Flt 3 ligand mobilizes dendritic cells ( DCs ) into blood , allowing generation in vivo of large numbers of DCs for immunotherapy . ^^^ Flt 3 ligand mobilized DCs ( FLDCs ) were isolated , activated with CD40L , loaded with antigenic peptides from influenza matrix protein , hepatitis B core antigen , NY ESO 1 , MAGE A 4 , and MAGE A 10 , and injected into patients with resected melanoma . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
On the other hand , Flt 3 ligand was less effective in enhancing DC reconstitution till 3 weeks post BMT . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
Lyn is renowned for its role in B cell antigen receptor and FcepsilonRI signaling ; however , it is becoming increasingly apparent that Lyn also functions in signal transduction from growth factor receptors including the receptors for GM CSF , IL 3 , IL 5 , SCF , erythropoietin , CSF 1 , G CSF , thrombopoietin and Flt 3 ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
OBJECTIVE : We have previously shown that Flt 3 ligand ( FL ) / Flt3 signaling regulates hematopoietic cell migration by modulating the SDF1alpha / CXCR4 signaling pathway . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
In the present study , we investigated whether 10 irradiated hematopoietic stem cells can be induced to undergo megakaryocytopoiesis and thrombopoiesis in vitro using cytokine combinations that have been demonstrated to be effective for conferring increased survival on irradiated human CD 34 ( + ) megakaryocytic progenitor cells ( colony forming unit megakaryocytes ; CFU Meg ) , such as thrombopoietin ( TPO ) , interleukin 3 ( IL 3 ) , stem cell factor and FLT 3 ligand . ^^^
Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
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Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
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Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
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Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
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Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
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Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
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Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
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Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
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Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
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Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
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Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
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Interacting proteins: P49771 and P36888	Pubmed	SVM Score :0.0
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