| Interacting proteins: P35754 and Q99683 |
Pubmed |
SVM Score :0.0 |
| Deletion mutants showed the C terminal portion of apoptosis signal regulating kinase 1 ( ASK 1 ) bound GRX , and glucose deprivation disrupted binding . ^^^ Treatment with l buthionine ( S , R ) sulfoximine reduced glutathione content by 99 % and prevented glucose deprivation induced dissociation of GRX from ASK 1 . ^^^ A thiol antioxidant , N acetyl l cysteine , or overexpression of an H ( 2 ) O ( 2 ) scavenger , catalase , inhibited glucose deprivation induced dissociation of GRX from ASK 1 . ^^^ GRX active site cysteine residues ( Cys ( 22 ) and Cys ( 25 ) ) were required for dissociation of GRX from ASK 1 during glucose deprivation . ^^^ Overexpression of GRX or catalase inhibited activation of ASK 1 SEK1 JNK 1 signaling during glucose deprivation . ^^^ |
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| Interacting proteins: P35754 and Q99683 |
Pubmed |
SVM Score :0.0 |
| Redox sensing molecules such as thioredoxin ( TRX ) and glutaredoxin ( GRX ) bind to apoptosis signal regulating kinase 1 ( ASK 1 ) and suppress its activation . ^^^ Glucose deprivation disrupted the interaction between TRX / GRX and ASK 1 and subsequently activated the ASK 1 stress activated protein kinase / extracellular signal regulated kinase kinase c Jun N terminal kinase 1 ( JNK 1 ) signal transduction pathway . ^^^ L Buthionine ( S , R ) sulphoximine , which decreases intracellular glutathione content , enhanced glucose deprivation induced activation of JNK 1 by promoting the dissociation of TRX , but not GRX , from ASK 1 . ^^^ Treatment of cells with exogenous glutathione disulphide ester resulted in the dissociation of GRX , but not TRX , from ASK 1 and the subsequent activation of JNK 1 . ^^^ Nonetheless , overexpression of calatase , an H ( 2 ) O ( 2 ) scavenger , inhibited JNK 1 activation and cytotoxicity as well as the dissociation of TRX and GRX from ASK 1 during combined glucose deprivation and L buthionine ( S , R ) sulphoximine treatment . ^^^ |
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| Interacting proteins: P35754 and Q99683 |
Pubmed |
SVM Score :0.0 |
| Recently , acute total glucose deprivation has been shown to cause activation of ASK 1 MEK MAPK signal transduction and dissociation of glutaredoxin ( GRX ) from apoptosis signal regulating kinase 1 ( ASK 1 ) . ^^^ Similar to JNK 1 activation , we also observed that low glucose induced ASK 1 activation , dissociation of GRX and thioredoxin ( TRX ) from ASK 1 , dimerization of ASK 1 , and association of Daxx and TRAF 2 with ASK 1 significantly occurred at a glucose concentration less than or equal to 0 . 01 mM . . ^^^ |
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