| Interacting proteins: P53985 and P35613 |
Pubmed |
SVM Score :0.91989886 |
| In addition , MCT 1 and MCT 4 have been shown to interact specifically with OX 47 ( CD 147 ) , a member of the immunoglobulin superfamily with a single transmembrane helix . 0.91989886^^^ |
|
| Interacting proteins: P53985 and P35613 |
Pubmed |
SVM Score :0.66146963 |
| Here we use co immunoprecipitation and chemical cross linking to demonstrate that CD 147 specifically interacts with MCT 1 and MCT 4 , two members of the proton linked monocarboxylate ( lactate ) transporter family that play a fundamental role in metabolism , but not with MCT 2 . 0.66146963^^^ |
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| Interacting proteins: P53985 and P35613 |
Pubmed |
SVM Score :1.0538421 |
| We show that MCT 1 associates with CD 147 at the apical plasma membrane in Caco 2 BBE cell monolayers . 1.0538421^^^ |
|
| Interacting proteins: P53985 and P35613 |
Pubmed |
SVM Score :0.62225823 |
| The interaction between gp 70 and MCT 2 was confirmed using fluorescence resonance energy transfer between the cyan fluorescent protein and yellow fluorescent protein tagged MCT 2 and gp 70 . pCMBS strongly inhibited lactate transport into rabbit erythrocytes , where MCT 1 interacts with CD 147 , but not into rat erythrocytes where it interacts with gp 70 . 0.62225823^^^ |
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| Interacting proteins: P53985 and P35613 |
Pubmed |
SVM Score :0.63578208 |
| Immunohistochemistry demonstrated that Bsg colocalized with MCT 1 in the cerebrum , retina , skeletal and cardiac muscle , duodenal epithelium , hepatic sinusoid , proximal uriniferous tubules , Leydig cells , and efferent ductule epithelium in WT mice . 0.63578208^^^ |
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| Interacting proteins: P53985 and P35613 |
Pubmed |
SVM Score :0.0 |
| However , in other cells MCT 1 and MCT 4 are intimately associated with an ancillary protein OX 47 ( also known as CD 147 ) . ^^^ |
|
| Interacting proteins: P53985 and P35613 |
Pubmed |
SVM Score :0.0 |
| Third , CD 147 , an MCT associated chaperone protein , which is closely colocalized with MCT 1 on acinar cell membranes , was absent from islet cell membranes . ^^^ |
|
| Interacting proteins: P53985 and P35613 |
Pubmed |
SVM Score :0.0 |
| Fluorescence resonance energy transfer studies on the interaction between the lactate transporter MCT 1 and CD 147 provide information on the topology and stoichiometry of the complex in situ . ^^^ The monocarboxylate ( lactate ) transporters MCT 1 and MCT 4 require the membrane spanning glycoprotein CD 147 for their correct plasma membrane expression and function . ^^^ We have successfully expressed CD 147 and MCT 1 tagged on their C or N termini with either the cyan ( CFP ) or yellow ( YFP ) variants of green fluorescent protein . ^^^ FRET was observed when either the C or N terminus of MCT 1 ( intracellular ) is tagged with CFP or YFP and co expressed with CD 147 tagged with YFP or CFP on the C terminus ( intracellular ) but not the N terminus ( extracellular ) . ^^^ Because FRET only occurs between two fluorophores if they are less than 100 A apart and in a suitable orientation , our data provide important information on the topology of CD 147 and MCT 1 within the plasma membrane . ^^^ |
|
| Interacting proteins: P53985 and P35613 |
Pubmed |
SVM Score :0.0 |
| After the detection of strong discrete granular cytoplasmic immunoreactivity in ASPS for monocarboxylate transporter 1 ( MCT 1 ) in the course of a broad immunohistochemical characterization of an MCT 1 antibody , we studied the expression of MCT 1 and its interacting partner , CD 147 , in a panel of 10 ASPS cases using appropriate antibodies . ^^^ We find that the periodic acid Schiff diastase resistant cytoplasmic granules of ASPS are strongly immunoreactive for MCT 1 and CD 147 . ^^^ Specifically , intense cytoplasmic granular positivity for MCT 1 and CD 147 was found in 7 of 10 and 8 of 10 ASPSs , respectively . ^^^ Western blot analysis of several ASPS cases confirmed that the protein reactive with the MCT 1 antibody and that reactive with the CD 147 antibody both migrated at the size expected for MCT 1 and CD 147 , respectively . ^^^ |
|
| Interacting proteins: P53985 and P35613 |
Pubmed |
SVM Score :0.0 |
| Human and mouse genetic expression of MCT members 1 , 2 , 3 , 4 and basigin , a chaperone protein of MCT 1 and MCT 4 , was qualitatively analysed using the reverse transcription nested polymerase chain reaction ( RT nested PCR ) in immature oocytes ( germinal vesicle stage ; GV ) , in non fertilised metaphase 2 ( MII ) oocytes and in embryos from 2 cell stage to blastocysts . ^^^ |
|
| Interacting proteins: P53985 and P35613 |
Pubmed |
SVM Score :0.0 |
| The amount of CD 147 , a chaperone necessary for the activity of MCT 1 , was lower in LT horses . ^^^ |
|
| Interacting proteins: P53985 and P35613 |
Pubmed |
SVM Score :0.0 |
| Western blot analysis of detergent soluble lysates prepared from wild type and Bsg ( / ) eyes confirmed that the levels of MCT 1 , MCT 3 , and MCT 4 protein were severely reduced in Bsg ( / ) mice . ^^^ RT PCR analyses of mRNA levels from wild type and Bsg ( / ) mice demonstrated that the MCT 1 transcript was expressed at normal levels in Bsg ( / ) mice . ^^^ CONCLUSIONS : In Bsg ( / ) mice , there is a severe reduction in accumulation of the MCT 1 and 3 proteins in the RPE and a concomitant reduction in MCT 1 and 4 in the neural retina supporting a role for 5A11 / basigin in the targeting of these transporters to the plasma membrane . ^^^ Recently , it was reported that targeting of MCT 1 and 4 to the plasma membrane requires association with 5A11 / basigin ( CD 147 ) . ^^^ |
|
| Interacting proteins: P53985 and P35613 |
Pubmed |
SVM Score :0.0 |
| Post translational regulation of MCT 1 and MCT 4 occurs , in part , by interaction with CD 147 , an accessory protein that is necessary for trafficking , localization , and functional expression of these transporters . ^^^ |
|
| Interacting proteins: P53985 and P35613 |
Pubmed |
SVM Score :0.0 |
| MCT 1 and its accessory protein CD 147 are differentially regulated by TSH in rat thyroid cells . ^^^ CD 147 colocalizes and coimmunoprecipitates with MCT 1 . ^^^ TSH enhances protein expression of both MCT 1 and CD 147 in FRTL 5 cells . ^^^ Whereas MCT 1 protein expression is controlled at the level of transcription , CD 147 protein expression is regulated by a posttranscriptional mechanism . ^^^ |
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| Interacting proteins: P53985 and P35613 |
Pubmed |
SVM Score :0.0 |
| We performed Western blotting using antibodies to cell membrane glucose transporter isoform GLUT 1 , inner mitochondrial constituent cytochrome oxidase , the monocarboxylate transporter protein chaperone CD 147 , as well as custom and commercially available MCT 1 antibodies . ^^^ |
|
| Interacting proteins: P53985 and P35613 |
Pubmed |
SVM Score :0.0 |
| CD 147 , a chaperone protein for MCT 1 and MCT 4 , was equally expressed by both membranes . ^^^ |
|
| Interacting proteins: P53985 and P35613 |
Pubmed |
SVM Score :0.0 |
| We have used the system to characterize the functional significance of interactions between the rat MCT 1 transporter and its ancillary protein CD 147 . ^^^ CD 147 was shown to improve trafficking of MCT 1 to the plasma membrane and its uptake activity . ^^^ |
|
| Interacting proteins: P53985 and P35613 |
Pubmed |
SVM Score :0.0 |
| In addition , expression of chaperone protein CD 147 , which is needed for the activity of MCT 1 and MCT 4 , was studied in the rumen of suckling calves . ^^^ Developing rumen of suckling calves expressed MCT 1 and MCT 4 and positive correlation was found between MCT 1 and CD 147 . ^^^ Both MCT 1 and CD 147 correlated also with age in calves less than 10 days . ^^^ |
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| Interacting proteins: P53985 and P35613 |
Pubmed |
SVM Score :0.0 |
| Proton coupled monocarboxylate transporters ( MCT ) MCT 1 , MCT 3 , and MCT 4 form heterodimeric complexes with the cell surface glycoprotein CD 147 and exhibit tissue specific polarized distributions that are essential for maintaining lactate and pH homeostasis . ^^^ RNA interference experiments show that MCT 1 promotes CD 147 maturation . ^^^ |
|
| Interacting proteins: P53985 and P35613 |
Pubmed |
SVM Score :0.0 |
| MCT 1 and MCT 4 require a monotopic ancillary protein , CD 147 , for expression of functional protein at the plasma membrane . ^^^ |
|
| Interacting proteins: P53985 and P35613 |
Pubmed |
SVM Score :0.0 |
| Colocalization of MCT 1 , CD 147 , and LDH in mitochondrial inner membrane of L 6 muscle cells : evidence of a mitochondrial lactate oxidation complex . ^^^ Therefore , by using a rat derived L 6 skeletal muscle cell line and confocal laser scanning microscopy ( CLSM ) , we examined the cellular locations of mitochondria , lactate dehydrogenase ( LDH ) , the lactate pyruvate transporter MCT 1 , and CD 147 , a purported chaperone protein for MCT 1 . ^^^ CLSM showed that LDH , MCT 1 , and CD 147 are colocalized with the mitochondrial reticulum . ^^^ Western blots showed that cytochrome oxidase ( COX ) , NADH dehydrogenase , LDH , MCT 1 , and CD 147 are abundant in mitochondrial fractions of L 6 cells . ^^^ Interactions among COX , MCT 1 , and CD 147 in mitochondria were confirmed by immunoblotting after immunoprecipitation . ^^^ |
|
| Interacting proteins: P53985 and P35613 |
Pubmed |
SVM Score :0.0 |
| We have examined the rapid effect of 1 , 25 dihydroxyvitamin D 3 [ 1 , 25 ( OH ) 2D3 ] on apical Na+ / H+ exchange activity in opossum kidney ( OK ) cells and in MCT cells ( a culture of simian virus 40 immortalized mouse cortical tubule cells ) grown on filter support . ^^^ Addition of 1 , 25 ( OH ) 2D3 ( 10 nM ) for 1 min increased apical Na+ / H+ exchange activity [ recovery from an acid load ; measured by 2 ' , 7 ' bis ( 2 carboxyethyl ) 5 ( 6 ) carboxyfluorescein ] in OK cells ( by 56 % ) and in MCT cells ( by 36 % ) . ^^^ |
|
| Interacting proteins: P53985 and P35613 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P53985 and P35613 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P53985 and P35613 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P53985 and P35613 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P53985 and P35613 |
Pubmed |
SVM Score :0.0 |
| NA |
|