| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| Treatment of MDR cells with an anti EMMPRIN antibody inhibited the activity of MMP 1 , MMP 2 , and MMP 9 . ^^^ |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| TCSF is a membrane bound glycoprotein of tumor cells that stimulates fibroblast collagenase production . ^^^ |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| In the present study , we demonstrated that coculture of basigin expressing human MM cells with dermal fibroblasts resulted in the induction of MMP 1 , MMP 2 , MMP 3 and MT 1 MMP production by fibroblasts and of melanoma cell invasion through a reconstituted basement membrane . ^^^ |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| Tumor cell derived collagenase stimulatory factor increases expression of interstitial collagenase , stromelysin , and 72 kDa gelatinase . ^^^ |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| Tumor cell derived collagenase stimulatory factor ( TCSF ) stimulates in vitro the biosynthesis of various matrix metalloproteinases involved in tumor invasion , such as interstitial collagenase , gelatinase A , and stromelysin 1 . ^^^ |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| We have previously isolated and purified a tumor cell surface protein , EMMPRIN ( extracellular matrix metalloproteinase inducer ) , which stimulates production of interstitial collagenase , gelatinase A , and stromelysin 1 by fibroblasts , and we have obtained cDNA clones that encode the EMMPRIN protein from LX 1 human lung carcinoma cells . ^^^ |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| Among these factors , extracellular matrix metalloproteinase inducer ( EMMPRIN ) has been shown to stimulate in vitro the fibroblast production of various MMPs such as interstitial collagenase ( MMP 1 ) , stromelysin 1 ( MMP 3 ) , and gelatinase A ( MMP 2 ) . ^^^ |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| Among these factors , extracellular matrix metalloproteinase inducer ( EMMPRIN ) is known to increase in vitro stromal cell production of MMP 1 , MMP 2 and MMP 3 . ^^^ |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| MMP 2 , MMP 9 , MT 1 MMP , and extracellular matrix metalloproteinase inducer ( EMMPRIN ) , TIMP 1 , TIMP 2 , TIMP 3 , and TIMP 4 mRNA were quantitated by real time RCR . ^^^ |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| CK 5 correlated with tissue inhibitor of metalloproteinase ( TIMP ) 1 , but not matrix metalloproteinase ( MMP ) 1 , MMP 2 , extracellular matrix metalloproteinase inducer , TIMP 2 or plasminogen activator inhibitor , indicating that antiproteolytic stimuli might be preponderant in these neoplasms . . ^^^ |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| PURPOSE : This study was conducted to determine the eventual presence and activity of EMMPRIN ( extracellular matrix metalloproteinase inducer CD 147 ) and interstitial collagenase MMP 1 in the cornea of keratoconus patients and their eventual interrelationship . ^^^ |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| We show that these transfected cells synthesize EMMPRIN that is extensively post translationally processed ; this recombinant EMMPRIN stimulates human fibroblast production of interstitial collagenase , stromelysin 1 , and gelatinase A ( 72 kDa type 4 collagenase ) . ^^^ |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| Here we focus on MMP 1 , showing that in lung tumors , MMP 1 ' s cognate mRNA is strongly expressed in stromal fibroblasts adjacent to EMMPRIN expressing tumor cells . ^^^ In vitro , EMMPRIN upregulates MMP 1 mRNA expression in a concentration dependent manner , with a peak accumulation at 24 h . ^^^ That p 38 activity was required for EMMPRIN stimulation of MMP 1 was evident from results showing that the p 38 inhibitor SB 203580 blocked this response . ^^^ |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| Double immunofluorescence labeling revealed EMMPRIN / MMP 1 double positive cells in lining like layers and the sublining area of interface tissue . ^^^ Our findings indicate that EMMPRIN expression is upregulated in interface tissue , and that locally accumulated EMMPRIN may modulate MMP 1 expression . ^^^ |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| EMMPRIN ( CD 147 ) , an inducer of matrix metalloproteinase synthesis , also binds interstitial collagenase to the tumor cell surface . ^^^ In this study , we have found that EMMPRIN not only stimulates the production of interstitial collagenase ( MMP 1 ) but also forms a complex with MMP 1 at the tumor cell surface . ^^^ Presentation of MMP 1 complexed to EMMPRIN at the tumor cell surface may be important in modifying the tumor cell pericellular matrix to promote invasion . . ^^^ |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| MMP 1 and MMP 3 production by synovial tissue was correlated with levels of expression of EMMPRIN mRNA , as detected by Northern blotting . ^^^ CONCLUSION : The expression of EMMPRIN stimulates the production of MMP 1 and MMP 3 in the synovial tissue of affected joints in RA . ^^^ |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| ECM inducer protein ( EMMPRIN ) ; membrane type MMP ( MT MMP ) ; and MMP 1 , 2 , and 9 were quantified by immunoblotting and densitometric scanning ( pixels ) . ^^^ |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| Mechanical stretch induces MMP 2 release and activation in lung endothelium : role of EMMPRIN . ^^^ Our results show that 1 ) cyclic stretch led to increased release and activation of MMP 2 and MMP 1 ; 2 ) the activation of MMP 2 was accompanied by an increase in membrane type 1 MMP ( MT 1 MMP ) and inhibited by a hydroxamic acid derived inhibitor of MMPs ( Prinomastat , AG 3340 ) ; and 3 ) the MMP 2 release and activation were preceded by an increase in production of extracellular MMP inducer ( EMMPRIN ) . ^^^ |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| ECM inducer protein ( EMMPRIN ) and MMP activator membrane type MMP ( MT 1 MMP ) , as well as MMP 1 , 2 , and 9 , were quantified by immunoblotting and densitometry ( pixels ) . ^^^ |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| DC SIGN positive macrophages adjacent to these T cells were located in close proximity to the cartilage degrading proteins extracellular MMP inducer ( EMMPRIN ) and MMP 1 . ^^^ Expression of DC SIGN and its ligand , ICAM 3 , is found in substantial amounts only in RA synovium , suggesting that their interaction is implicated in the additional activation of synovial macrophages that leads to the production of EMMPRIN and MMP 1 . . ^^^ |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| EMMPRIN was co expressed with MMP 1 ( P < 0 . 001 ) , MMP 9 ( P = 0 . 006 ) and the alphav ( P = 0 . 013 ) and beta 1 ( P = 0 . 029 ) integrin subunits . ^^^ |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| MMP 1 , MMP 2 , MMP 9 and EMMPRIN expression was studied using immunocytochemistry ( ICC ) and mRNA in situ hybridization ( ISH ) . ^^^ EMMPRIN expression correlated with MMP 1 ( P < 0 . 001 ) , MMP 2 ( P = 0 . 042 ) and MMP 9 ( P = 0 . 029 ) expression , as well as with ERK activity ( P = 0 . 001 ) . ^^^ |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| Furthermore , EMMPRIN colocalized with MMP 1 / collagenase 1 in the glandular epithelium in vivo . ^^^ Moreover , EMMPRIN may be involved in ECM breakdown at the interface between endometrial cells and ECM by using EMMPRIN bound MMP 1 . . ^^^ |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| Western blot analysis demonstrated that MMP 1 and MMP 2 induce the cleavage of the extracellular domain from cell associated EMMPRIN . ^^^ MMP 1 and MMP 2 synthesis was upregulated by oxLDL , and , in addition , we have shown that soluble EMMPRIN , isolated from macrophage supernatants , increased MMP 1 and MMP 2 synthesis in HCA SMC . ^^^ |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| Tissue levels of extracellular matrix inducer protein ( EMMPRIN ) , MMP activator protein ( MT 1 MMP ) , MMP 1 , MMP 2 , and MMP 9 , as well as tissue inhibitors of MMPs ( TIMP 1 and TIMP 2 ) were assessed by immunoblotting and yielded a significant decrease in MMP 9 , EMMPRIN , and MT 1 MMP levels in hypertension . ^^^ |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| Expression of matrix metalloproteinases [ MMPs ( MMP 1 , MMP 2 , MMP 9 , and MMP 14 ) ] , the MMP inhibitor tissue inhibitor of metalloproteinase 2 , the MMP inducer EMMPRIN , the 67 kDa LR , the alpha 6 integrin subunit , and the transcription factors AP 2 , Ets 1 , and PEA 3 was studied using immunohistochemistry , mRNA in situ hybridization , reverse transcription polymerase chain reaction , zymography , and flow cytometry . ^^^ In situ hybridization demonstrated higher MMP 2 ( P = 0 . 007 ) , PEA 3 ( P = 0 . 038 ) , and EMMPRIN ( P = 0 . 026 ) mRNA expression in effusions . ^^^ |
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| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| This coincided with MMP 2 induction that co localized with EMMPRIN at the epithelio stromal boundary . ^^^ The role of epithelial stromal interaction in MMP induction was investigated in an in vitro co culture system and demonstrated an induction and co localization of EMMPRIN and MMP 2 in the fibroblasts at the interface with epithelial cells . ^^^ Direct contact of fibroblasts with EMMPRIN containing purified epithelial cell membranes also induced MMP 1 , MMP 2 , and EMMPRIN and this was inhibited by a blocking anti EMMPRIN antibody , suggesting that EMMPRIN was primarily responsible for this induction . ^^^ |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| Incubation of cultured human coronary smooth muscle cells with human HGF ( 1 100 ng / ml ) did not significantly alter cell migration and collagen 1 , fibronectin , EMMPRIN , MMP 1 , MMP 2 and MMP 9 release . ^^^ |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| EMMPRIN treatment ( 0 . 5 microg / ml ) of human uterine fibroblast cells stimulated MMP 1 ( 5 . 23 fold ) and MMP 2 ( 8 . 55 fold ) , but not MMP 3 , mRNA levels over levels in control cells ( P < 0 . 05 ) . ^^^ IL 1beta stimulated MMP 1 ( 5 . 6 fold ) , MMP 2 ( 2 . 8 fold ) , and MMP 3 ( 75 fold ) gene expression , but not EMMPRIN , over levels in control cells ( P < 0 . 05 ) . ^^^ Both EMMPRIN and IL 1beta treatments stimulated MMP 1 , 2 , and 3 , but not EMMPRIN protein secretion , with 0 . 5 microg / ml producing the greatest response . ^^^ |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| CONCLUSION : These results suggest that emmprin and MT 1 MMP may be malignant potential related proteins in PNSTs , and that MMP 1 and 9 may help differentiation between schwannoma and neurofibroma , especially in their plexiform types . . ^^^ |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| This was achieved in two ways : ( a ) a polyclonal antibody was raised against the 58 kDa protein , after excision from an SDS PAGE gel , and shown to inhibit the stimulation of fibroblast collagenase production by TCSF preparations ; ( b ) the 58 kDa protein was eluted from a transblot of purified TCSF and shown to stimulate fibroblast collagenase production . ^^^ However , TCSF 1 but not TCSF 2 stimulated fibroblast collagenase production , confirming that the 58 kDa protein is the major active component of TCSF preparations . . ^^^ |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35613 and P03956 |
Pubmed |
SVM Score :0.0 |
| NA |
|