| Interacting proteins: P35442 and P08253 |
Pubmed |
SVM Score :0.72963774 |
| Although MMP 2 interacted with TSP 1 and TSP 2 via its gelatin binding domain or a closely mapping site , neither TSP 1 nor TSP 2 was degraded by MMP 2 in vitro . 0.72963774^^^ |
|
| Interacting proteins: P35442 and P08253 |
Pubmed |
SVM Score :1.2606239 |
| Finally , MMP 2 was shown to interact with TSP 2 in a direct binding plate assay . 1.2606239^^^ |
|
| Interacting proteins: P35442 and P08253 |
Pubmed |
SVM Score :0.62211716 |
| Our laboratory has established that TSP 2 binds matrix metalloproteinase 2 ( MMP 2 ) and that the adhesive defect in TSP 2 null fibroblasts results from increased MMP 2 activity . 0.62211716^^^ |
|
| Interacting proteins: P35442 and P08253 |
Pubmed |
SVM Score :0.0 |
| We have recently shown that the adhesive defect observed in dermal fibroblasts derived from thrombospondin 2 ( TSP 2 ) null mice results from an increase in matrix metalloproteinase 2 ( MMP 2 ) levels ( Yang , Z . , Kyriakides , T . ^^^ Adhesion was restored by replacement of TSP 2 and by inhibitors of MMP 2 activity . ^^^ In pursuing the observation that TSP 2 and MMP 2 interact , we now demonstrate that this interaction is required for optimal clearance of extracellular MMP 2 by fibroblasts . ^^^ Since TSP 2 is known to be endocytosed by the scavenger receptor , low density lipoprotein receptor related protein ( LRP ) , we determined whether interference with LRP function affected fibroblast adhesion and / or extracellular MMP 2 levels . ^^^ Addition of heparin , which competes for the binding of TSP 2 to LRP coreceptor proteoglycans , inhibited adhesion of control but not TSP 2 null cells , and a blocking antibody to LRP as well as the LRP inhibitor , receptor associated protein , also inhibited adhesion and increased MMP 2 levels only in control fibroblasts . ^^^ |
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| Interacting proteins: P35442 and P08253 |
Pubmed |
SVM Score :0.0 |
| The alterations in neovascularization , dense tissue invasion , and MMP 2 in TSP 2 null mice coincided with the deposition of TSP 2 in the extracellular matrix of wild type animals . ^^^ In addition , they provide in vivo evidence for a newly proposed function of TSP 2 as a modulator of extracellular MMP 2 levels . . ^^^ |
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| Interacting proteins: P35442 and P08253 |
Pubmed |
SVM Score :0.0 |
| This study was undertaken to quantitate mRNA levels in normal and malignant cervical tissues of nine selected genes ( VEGF ( 121 ) , VEGF ( 165 ) , VEGF ( 189 ) , VEGF C , eIF 4E , b FGF , TSP 2 , MMP 2 and MMP 9 ) implicated in the above processes using real time quantitative RT PCR . ^^^ Co expression pattern analysis in normal cervical tissue revealed highly significant co expressions ( P < 0 . 0001 ) between TSP 2 and most other genes analysed ( VEGF ( 121 ) , VEGF ( 165 ) , VEGF C , b FGF and MMP 2 ) . ^^^ In cervical cancer , TSP 2 appears only to be highly co expressed with MMP 2 ( P < 0 . 0001 ) . ^^^ |
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| Interacting proteins: P35442 and P08253 |
Pubmed |
SVM Score :0.0 |
| The transformants with the human TSP 2 gene overexpression showed a down regulation of matrix metalloproteinase 2 ( MMP 2 ) and MMP 9 in comparison to those with vector control . ^^^ Protein production of MMP 2 and MMP 9 decreased in the transformants overexpressing the TSP 2 gene . ^^^ |
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| Interacting proteins: P35442 and P08253 |
Pubmed |
SVM Score :0.0 |
| As determined by immunohistochemistry , the amount of matrix metalloproteinase 2 ( MMP 2 ) in the cervix of TSP 2 null mice increased 11 fold on Day 14 of pregnancy and 19 fold on Day 18 . ^^^ |
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| Interacting proteins: P35442 and P08253 |
Pubmed |
SVM Score :0.0 |
| We suggest that the delay in expression of TSP 2 and MMP 2 in the wounds of aged mice could contribute to their impaired rate of wound healing . . ^^^ |
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| Interacting proteins: P35442 and P08253 |
Pubmed |
SVM Score :0.0 |
| In work that stems largely from our own laboratory , we show that pericellular levels of the matrix metalloproteinase , MMP 2 , are controlled to a large extent by TSP 2 ( and potentially also by TSP 1 ) , and that elevated levels of MMP 2 are likely to account in part for defects as diverse as reduced cellular adhesion , abnormal collagen fibril structure , and increased endothelial cell and vascular proliferation . . ^^^ |
|
| Interacting proteins: P35442 and P08253 |
Pubmed |
SVM Score :0.0 |
| By using immunohistochemistry , Western blot , and real time PCR the expression patterns of TSP 1 , TSP 2 , CD 36 , CD 47 , MMP 2 , and MMP 9 were investigated in normal rat prostate tissue and five malignant Dunning sublines tissue . ^^^ |
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| Interacting proteins: P35442 and P08253 |
Pubmed |
SVM Score :0.0 |
| Mechanistically , matrix metalloproteinase ( MMP ) 2 has been associated with neoangiogenesis and it has been proposed that the levels of available MMP 2 may be down regulated by formation of a complex with TSP 2 that is internalized by low density lipoprotein receptor related protein 1 ( LRP 1 ) . ^^^ We found elevated expression of MMP 2 and MMP 9 in tumors propagated in TSP 2 ( / ) mice , with a preferential localization in the microvasculature . ^^^ In wild type mice , MMP 2 was coexpressed with TSP 2 in the tumor microvasculature . ^^^ In vitro , addition of recombinant ( rec ) TSP 2 to mouse brain microvessel endothelial cells reduced MMP 2 levels and invasion through mechanisms that could be inhibited by a competitive inhibitor of ligand binding to LRP 1 or by siLRP 1 . ^^^ Thus , the antiangiogenic activity of TSP 2 is capable of inhibiting the growth of gliomas in part by reducing the levels of MMP 2 in the tumor microvasculature . ^^^ |
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| Interacting proteins: P35442 and P08253 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35442 and P08253 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35442 and P08253 |
Pubmed |
SVM Score :0.0 |
| NA |
|
| Interacting proteins: P35442 and P08253 |
Pubmed |
SVM Score :0.0 |
| NA |
|