| Interacting proteins: P40938 and P35251 |
Pubmed |
SVM Score :0.0 |
| Here we report the cloning , by either peptide sequencing or by sequence similarity to the human cDNAs , of the S . cerevisiae genes RFC 1 , RFC 2 , RFC 3 , RFC 4 , and RFC 5 . ^^^ |
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| Interacting proteins: P40938 and P35251 |
Pubmed |
SVM Score :0.0 |
| However , Rfc4p formed a tight complex with the product of the RFC 3 gene which encodes the ATPase of RF C . . ^^^ |
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| Interacting proteins: P40938 and P35251 |
Pubmed |
SVM Score :0.0 |
| Molecular cloning and expression of the Saccharomyces cerevisiae RFC 3 gene , an essential component of replication factor C . ^^^ The gene encoding the 40 kDa subunit of the Saccharomyces cerevisiae RF C ( RFC 3 ) has been cloned . ^^^ The RFC 3 gene is required for yeast cell growth and has been mapped to the left arm of chromosome 14 . ^^^ The deduced amino acid sequence of the RFC 3 gene shows a high homology to the 36 , 37 , and 40 kDa subunits of human RF C ( also called activator 1 ) , with the highest homology to the 36 kDa subunit . ^^^ The steady state mRNA levels of RFC 3 do not change significantly during the mitotic cell cycle of yeast . ^^^ |
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| Interacting proteins: P40938 and P35251 |
Pubmed |
SVM Score :0.0 |
| In independent screens using three different Cs ( ) mutants , we identified spontaneously arising dominant suppressor mutations in the RFC 3 gene . ^^^ RFC 1 , RFC 2 , and RFC 3 encode three of the five subunits of the replication factor C complex , which is required to load PCNA onto DNA in reconstituted DNA replication reactions . ^^^ Biochemical analysis of the wild type and mutant PCNA and RFC 3 proteins shows that mutant RFC3p enhances the production of long DNA products in pol delta dependent DNA synthesis , which is consistent with an increase in processivity . . ^^^ |
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| Interacting proteins: P40938 and P35251 |
Pubmed |
SVM Score :0.0 |
| The 40 kDa species was found by mass spectrometry to contain Rfc 2 and Rfc 3 , subunits of replication factor C ( RFC ) , a five subunit protein that is required for the loading of polymerases onto DNA during replication and repair [ 3 ] . ^^^ These experiments showed that the small RFC proteins , Rfc 2 , Rfc 3 , Rfc 4 and Rfc 5 , interacted with Rad 24 , whereas the Rfc 1 subunit did not . ^^^ |
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| Interacting proteins: P40938 and P35251 |
Pubmed |
SVM Score :0.0 |
| The Schizosaccharomyces pombe rfc3+ gene encodes a homologue of the human hRFC 36 and Saccharomyces cerevisiae Rfc 3 subunits of replication factor C . ^^^ Here we describe the cloning of the S . pombe rfc3+ gene , encoding a homologue of the S . cerevisiae Rfc 3 and human hRFC 36 proteins . ^^^ The spliced ORF is predicted to encode a 342 amino acid protein that is approximately 50 % identical at the amino acid sequence level to the S . cerevisiae Rfc 3 and human hRFC 36 proteins . ^^^ Loss of rfc3+ function can be rescued by heterologous expression of either the S . cerevisiae Rfc 3 or human hRFC 36 proteins in S . pombe . . ^^^ |
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| Interacting proteins: P40938 and P35251 |
Pubmed |
SVM Score :0.0 |
| Rad 24 is structurally related to replication factor C ( RFC ) subunits and associates with RFC subunits Rfc 2 , Rfc 3 , Rfc 4 , and Rfc 5 . rad24Delta mutants are defective in all the G ( 1 ) , S , and G ( 2 ) / M phase DNA damage checkpoints , whereas the rfc 5 1 mutant is impaired only in the S phase DNA damage checkpoint . ^^^ |
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| Interacting proteins: P40938 and P35251 |
Pubmed |
SVM Score :0.0 |
| The ATP binding domains of Rfc 2 , Rfc 3 , and Rfc 4 are essential for DNA recognition and clamp loading . ^^^ The conserved lysine in the Walker A motif of the ATP binding domain encoded by the yeast RFC 1 , RFC 2 , RFC 3 , and RFC 4 genes was mutated to glutamic acid . ^^^ In contrast , complexes containing either a rfc 2 K71E mutation or a rfc 3 K59E mutation were severely impaired in ATPase and clamp loading activity . ^^^ Mutant RFC complexes containing rfc 2 K71R or rfc 3 K59R , carrying a conservative lysine > arginine mutation , had much milder clamp loading defects that could be partially ( rfc 2 K71R ) or completely ( rfc 3 K59R ) suppressed at high ATP concentrations . . ^^^ |
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| Interacting proteins: P40938 and P35251 |
Pubmed |
SVM Score :0.0 |
| Rad 24 is structurally related to subunits of the replication factor C ( RFC ) complex , and forms an RFC related complex with Rfc 2 , Rfc 3 , Rfc 4 , and Rfc 5 . ^^^ We also show that Chl 12 interacts physically with Rfc 2 , Rfc 3 , Rfc 4 , and Rfc 5 and forms an RFC related complex which is distinct from the RFC and RAD 24 complexes . ^^^ |
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| Interacting proteins: P40938 and P35251 |
Pubmed |
SVM Score :0.0 |
| To determine the relative importance of these sites for proper DNA metabolism in the cell , the conserved lysine in the Walker A motif of RFC 1 , RFC 2 , RFC 3 , or RFC 4 was mutated to either arginine or glutamic acid . ^^^ A glutamic acid substitution resulted in lethality in RFC 2 and RFC 3 but not in RFC 1 or RFC 4 . ^^^ |
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| Interacting proteins: P40938 and P35251 |
Pubmed |
SVM Score :0.0 |
| Combining the modeled structures for each RFC subunit with known structural , biochemical , and genetic data , we propose detailed models of how two of the RFC subunits , RFC 1 and RFC 3 , interact with the C terminal regions of PCNA . ^^^ RFC 1 is predicted to bind PCNA similarly to the p 21 PCNA interaction , while the RFC 3 PCNA binding is proposed to be similar to the E . coli delta beta interaction . ^^^ |
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| Interacting proteins: P40938 and P35251 |
Pubmed |
SVM Score :0.0 |
| We find that the RFC ( 3 / 4 ) complex is a DNA dependent ATPase , and we use this activity to determine that RFC 3 supplies a catalytic arginine to the ATP site of RFC 4 . ^^^ Furthermore , the RFC ( 2 / 3 ) and RFC ( 3 / 4 ) subassemblies bind stably to PCNA , yet neither RFC 2 nor RFC 4 bind tightly to PCNA , indicating that RFC 3 forms a strong contact point to PCNA . ^^^ |
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| Interacting proteins: P40938 and P35251 |
Pubmed |
SVM Score :0.0 |
| Proteins required for this pathway include Rad 17 , Rad 9 , Hus 1 , Rad 1 , and Rfc 2 , Rfc 3 , Rfc 4 , and Rfc 5 . ^^^ Sequence similarities suggest the biochemical functions of an RSR ( Rad 17 Rfc2 Rfc 3 Rfc4 Rfc 5 ) complex and an RHR heterotrimer ( Rad 1 Hus1 Rad 9 ) may be similar to that of RFC and PCNA , respectively . ^^^ |
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| Interacting proteins: P40938 and P35251 |
Pubmed |
SVM Score :0.0 |
| Following previous studies on the RFC 3 homologue from rice ( Oryza sativa L . cv . ^^^ |
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| Interacting proteins: P40938 and P35251 |
Pubmed |
SVM Score :0.0 |
| In that system , three ATP molecules bound to the Rfc 2 , Rfc 3 , and Rfc 4 subunits are necessary and sufficient for efficient loading of PCNA , whereas ATP binding to Rfc 1 is not required . ^^^ |
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| Interacting proteins: P40938 and P35251 |
Pubmed |
SVM Score :0.0 |
| Loading of PCNA onto DNA at template primer junctions is performed in an ATP dependent process by replication factor C ( RFC ) , a heteropentameric AAA+ protein complex consisting of the Rfc 1 , Rfc 2 , Rfc 3 , Rfc 4 , and Rfc 5 subunits . ^^^ |
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| Interacting proteins: P40938 and P35251 |
Pubmed |
SVM Score :0.0 |
| Similar results were obtained with antisera to the clamp loader proteins Rfc 3 and Rfc 4 , and to PCNA , i . e . ^^^ |
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| Interacting proteins: P40938 and P35251 |
Pubmed |
SVM Score :0.0 |
| NA |
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