| Interacting proteins: P35251 and P40937 |
Pubmed |
SVM Score :0.0 |
| Here we report the cloning , by either peptide sequencing or by sequence similarity to the human cDNAs , of the S . cerevisiae genes RFC 1 , RFC 2 , RFC 3 , RFC 4 , and RFC 5 . ^^^ |
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| Interacting proteins: P35251 and P40937 |
Pubmed |
SVM Score :0.0 |
| We here report the identification of a fifth RF C subunit from Saccharomyces cerevisiae , encoded by the RFC 5 ( YBR 0810 ) gene . ^^^ Like the other four RFC genes , the RFC 5 gene is essential for yeast viability , indicating an essential function for each subunit . ^^^ RFC 5 mRNA is expressed at steady state levels throughout the mitotic cell cycle . ^^^ Overproduction of RF C activity in yeast is dependent on overexpression of the RFC 5 gene together with overexpression of the RFC 1 4 genes , indicating that the RFC 5 gene product forms an integral subunit of this replication factor . . ^^^ |
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| Interacting proteins: P35251 and P40937 |
Pubmed |
SVM Score :0.0 |
| Rfc 5 , a replication factor C component , is required for regulation of Rad 53 protein kinase in the yeast checkpoint pathway . ^^^ The RFC 5 gene encodes a small subunit of replication factor C ( RFC ) complex in Saccharomyces cerevisiae . ^^^ We have previously shown that a temperature sensitive ( ts ) rfc 5 1 mutation is impaired in the S phase checkpoint . ^^^ In this report , we show that the rfc 5 1 mutation is sensitive to DNA damaging agents . ^^^ RFC 5 is necessary for slowing the S phase progression in response to DNA damage . ^^^ |
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| Interacting proteins: P35251 and P40937 |
Pubmed |
SVM Score :0.0 |
| It was also shown that the rfc 2 1 mutation is synthetically lethal with either the cdc 44 1 or rfc 5 1 mutation and that the restrictive temperature of rfc 2 1 mutant cells can be lowered by combining either with the cdc 2 2 or pol 2 11 mutation . ^^^ Finally , it was shown that the temperature sensitive cell growth phenotype and checkpoint defect of the rfc 2 1 mutation can be suppressed by a multicopy plasmid containing the RFC 5 gene . ^^^ These results suggest that the RFC 2 gene product interacts with the CDC44 / RFC1 and RFC 5 gene products in the RF C complex and with both DNA polymerases delta and epsilon during chromosomal DNA replication . . ^^^ |
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| Interacting proteins: P35251 and P40937 |
Pubmed |
SVM Score :0.0 |
| Functional and physical interaction between Rad 24 and Rfc 5 in the yeast checkpoint pathways . ^^^ The RFC 5 gene encodes a small subunit of replication factor C ( RFC ) complex in Saccharomyces cerevisiae and has been shown to be required for the checkpoints which respond to replication block and DNA damage . ^^^ Here we describe the isolation of RAD 24 , known to play a role in the DNA damage checkpoint , as a dosage dependent suppressor of rfc 5 1 . ^^^ RAD 24 overexpression suppresses the sensitivity of rfc 5 1 cells to DNA damaging agents and the defect in DNA damage induced Rad 53 phosphorylation . ^^^ Rad 24 , like Rfc 5 , is required for the regulation of Rad 53 phosphorylation in response to DNA damage . ^^^ |
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| Interacting proteins: P35251 and P40937 |
Pubmed |
SVM Score :0.0 |
| These experiments showed that the small RFC proteins , Rfc 2 , Rfc 3 , Rfc 4 and Rfc 5 , interacted with Rad 24 , whereas the Rfc 1 subunit did not . ^^^ |
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| Interacting proteins: P35251 and P40937 |
Pubmed |
SVM Score :0.0 |
| Rfc 5 , in cooperation with rad 24 , controls DNA damage checkpoints throughout the cell cycle in Saccharomyces cerevisiae . ^^^ RAD 24 and RFC 5 are required for DNA damage checkpoint control in the budding yeast Saccharomyces cerevisiae . ^^^ Rad 24 is structurally related to replication factor C ( RFC ) subunits and associates with RFC subunits Rfc 2 , Rfc 3 , Rfc 4 , and Rfc 5 . rad24Delta mutants are defective in all the G ( 1 ) , S , and G ( 2 ) / M phase DNA damage checkpoints , whereas the rfc 5 1 mutant is impaired only in the S phase DNA damage checkpoint . ^^^ Although neither rfc 5 1 nor rad 24 K115R single mutants are defective in the G ( 1 ) and G ( 2 ) / M phase DNA damage checkpoints , rfc 5 1 rad 24 K115R double mutants become defective in these checkpoints . ^^^ Coimmunoprecipitation experiments revealed that Rad 24 ( K115R ) fails to interact with the RFC proteins in rfc 5 1 mutants . ^^^ |
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| Interacting proteins: P35251 and P40937 |
Pubmed |
SVM Score :0.0 |
| Allele specific interactions between the yeast RFC 1 and RFC 5 genes suggest a basis for RFC subunit subunit interactions . ^^^ We isolated and characterized mutations in the RFC 5 gene that could suppress the cold sensitive phenotype of rfc 1 1 mutants . ^^^ Analysis of the RFC 5 suppressors revealed that they could not suppress the elongated telomere phenotype , the sensitivity to DNA damaging agents , or the mutator phenotype of rfc 1 1 mutants . ^^^ Unlike the checkpoint defective rfc 5 1 mutation , the RFC 5 suppressor mutations did not interfere with the methylmethane sulfonate or hydroxyurea induced phosphorylation of Rad53p . ^^^ |
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| Interacting proteins: P35251 and P40937 |
Pubmed |
SVM Score :0.0 |
| The rfc 4 2 mutant itself is sensitive to hydroxyurea , and like rfc 2 and rfc 5 strains , it exhibits defects in the DNA replication block and intra S checkpoints . ^^^ |
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| Interacting proteins: P35251 and P40937 |
Pubmed |
SVM Score :0.0 |
| Rad 24 is structurally related to subunits of the replication factor C ( RFC ) complex , and forms an RFC related complex with Rfc 2 , Rfc 3 , Rfc 4 , and Rfc 5 . ^^^ The rad24Delta mutation enhances the defect of rfc 5 1 in the DNA replication block checkpoint , implicating RAD 24 in this checkpoint . ^^^ We also show that Chl 12 interacts physically with Rfc 2 , Rfc 3 , Rfc 4 , and Rfc 5 and forms an RFC related complex which is distinct from the RFC and RAD 24 complexes . ^^^ |
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| Interacting proteins: P35251 and P40937 |
Pubmed |
SVM Score :0.0 |
| Additional sequence and structure analysis , supported by experimental data , suggests that RFC 5 might be the third clamp loader subunit to bind the equivalent PCNA region . ^^^ |
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| Interacting proteins: P35251 and P40937 |
Pubmed |
SVM Score :0.0 |
| Loading of PCNA onto DNA at template primer junctions is performed in an ATP dependent process by replication factor C ( RFC ) , a heteropentameric AAA+ protein complex consisting of the Rfc 1 , Rfc 2 , Rfc 3 , Rfc 4 , and Rfc 5 subunits . ^^^ |
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| Interacting proteins: P35251 and P40937 |
Pubmed |
SVM Score :0.0 |
| Cells carrying a temperature sensitive mutation in the CTD , rfc 1 44 , arrest with incompletely replicated chromosomes , are sensitive to DNA damaging agents , are synthetically lethal with other DNA replication mutants , and can be suppressed by mutations in rfc 5 . ^^^ |
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| Interacting proteins: P35251 and P40937 |
Pubmed |
SVM Score :0.0 |
| The Rfc 5 subunit is required to open PCNA . ^^^ Rfc 5 is positioned opposite the PCNA interface from Rfc 1 , and therefore , its action with Rfc 2 in opening PCNA indicates that PCNA is opened from the opposite side of the interface that the E . coli delta wrench acts upon . ^^^ |
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| Interacting proteins: P35251 and P40937 |
Pubmed |
SVM Score :0.0 |
| Proteins required for this pathway include Rad 17 , Rad 9 , Hus 1 , Rad 1 , and Rfc 2 , Rfc 3 , Rfc 4 , and Rfc 5 . ^^^ Sequence similarities suggest the biochemical functions of an RSR ( Rad 17 Rfc2 Rfc 3 Rfc4 Rfc 5 ) complex and an RHR heterotrimer ( Rad 1 Hus1 Rad 9 ) may be similar to that of RFC and PCNA , respectively . ^^^ |
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| Interacting proteins: P35251 and P40937 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P35251 and P40937 |
Pubmed |
SVM Score :0.0 |
| NA |
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