| Interacting proteins: P35251 and P35250 |
Pubmed |
SVM Score :0.0 |
| The Rad 24 protein , which is structurally related to the RFC subunits , interacts physically with RFC subunits Rfc 2 and Rfc 5 and cosediments with Rfc 5 . ^^^ |
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| Interacting proteins: P35251 and P35250 |
Pubmed |
SVM Score :0.0 |
| Here we report the cloning , by either peptide sequencing or by sequence similarity to the human cDNAs , of the S . cerevisiae genes RFC 1 , RFC 2 , RFC 3 , RFC 4 , and RFC 5 . ^^^ |
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| Interacting proteins: P35251 and P35250 |
Pubmed |
SVM Score :0.0 |
| The RFC 2 gene encoding a subunit of replication factor C of Saccharomyces cerevisiae . ^^^ We have isolated a gene , RFC 2 , that appears to be a component of the yeast RF C . ^^^ The RFC 2 gene is located on chromosome 10 of S . cerevisiae and is essential for cell growth . ^^^ Disruption of the RFC 2 gene led to a dumbbell shaped terminal morphology , common to mutants having a defect in chromosomal DNA replication . ^^^ The steady state levels of RFC 2 mRNA fluctuated less during the cell cycle than other genes involved in DNA replication . ^^^ |
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| Interacting proteins: P35251 and P35250 |
Pubmed |
SVM Score :0.0 |
| The RFC 2 gene , encoding the third largest subunit of the replication factor C complex , is required for an S phase checkpoint in Saccharomyces cerevisiae . ^^^ The RFC 2 gene encodes the third largest subunit of the RF C complex . ^^^ To elucidate the role of this subunit in DNA metabolism , we isolated a thermosensitive mutation ( rfc 2 1 ) in the RFC 2 gene . ^^^ It was shown that mutant cells having the rfc 2 1 mutation exhibit ( 1 ) temperature sensitive cell growth ; ( 2 ) defects in the integrity of chromosomal DNA at restrictive temperatures ; ( 3 ) progression through cell cycle without definitive terminal morphology and rapid loss of cell viability at restrictive temperatures ; ( 4 ) sensitivity to hydroxyurea , methyl methanesulfonate , and UV light ; and ( 5 ) increased rate of spontaneous mitotic recombination and chromosome loss . ^^^ These phenotypes of the mutant suggest that the RFC 2 gene product is required not only for chromosomal DNA replication but also for a cell cycle checkpoint . ^^^ |
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| Interacting proteins: P35251 and P35250 |
Pubmed |
SVM Score :0.0 |
| Here we describe the genetic analysis of the rfc 2 +gene of the fission yeast Schizosaccharomyces pombe encoding a structural homologue of the budding yeast Rfc2p and human hRFC 37 proteins . ^^^ Deletion of the rfc 2 + gene from the chromosome is lethal but does not result in the checkpoint dependent cell cycle arrest seen in cells deleted for the gene encoding PCNA or for those genes encoding subunits of either Pol delta or Pol straightepsilon . ^^^ Instead , rfc 2 Delta cells proceed into mitosis with incompletely replicated DNA , indicating that the DNA replication checkpoint is inactive under these conditions . ^^^ |
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| Interacting proteins: P35251 and P35250 |
Pubmed |
SVM Score :0.0 |
| In addition , dominant suppressor mutations were identified in the RFC 1 and RFC 2 genes using a single pol 30 mutant . ^^^ RFC 1 , RFC 2 , and RFC 3 encode three of the five subunits of the replication factor C complex , which is required to load PCNA onto DNA in reconstituted DNA replication reactions . ^^^ |
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| Interacting proteins: P35251 and P35250 |
Pubmed |
SVM Score :0.0 |
| The 40 kDa species was found by mass spectrometry to contain Rfc 2 and Rfc 3 , subunits of replication factor C ( RFC ) , a five subunit protein that is required for the loading of polymerases onto DNA during replication and repair [ 3 ] . ^^^ These experiments showed that the small RFC proteins , Rfc 2 , Rfc 3 , Rfc 4 and Rfc 5 , interacted with Rad 24 , whereas the Rfc 1 subunit did not . ^^^ |
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| Interacting proteins: P35251 and P35250 |
Pubmed |
SVM Score :0.0 |
| Here we report the down regulated mRNA expression of the 40 kDa subunit of replication factor C ( RFC p 40 or RFC 2 ) , which is an essential processive factor for proliferating cellular nuclear antigen dependent DNA replication during neonatal myocardial development . . ^^^ |
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| Interacting proteins: P35251 and P35250 |
Pubmed |
SVM Score :0.0 |
| Rad 24 is structurally related to replication factor C ( RFC ) subunits and associates with RFC subunits Rfc 2 , Rfc 3 , Rfc 4 , and Rfc 5 . rad24Delta mutants are defective in all the G ( 1 ) , S , and G ( 2 ) / M phase DNA damage checkpoints , whereas the rfc 5 1 mutant is impaired only in the S phase DNA damage checkpoint . ^^^ |
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| Interacting proteins: P35251 and P35250 |
Pubmed |
SVM Score :0.0 |
| Rad 17 is able to complex in vivo with an Rfc small subunit , Rfc 2 , but not with Rfc 1 . ^^^ Mutant Rad 17 ( K118E ) protein also has reduced ability to complex with Rfc 2 , suggesting that Lys ( 118 ) of Rad 17 plays a role in Rad 17 Rfc small subunit complex formation and chromatin association . ^^^ |
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| Interacting proteins: P35251 and P35250 |
Pubmed |
SVM Score :0.0 |
| The rfc 4 2 mutant itself is sensitive to hydroxyurea , and like rfc 2 and rfc 5 strains , it exhibits defects in the DNA replication block and intra S checkpoints . ^^^ |
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| Interacting proteins: P35251 and P35250 |
Pubmed |
SVM Score :0.0 |
| To determine the relative importance of these sites for proper DNA metabolism in the cell , the conserved lysine in the Walker A motif of RFC 1 , RFC 2 , RFC 3 , or RFC 4 was mutated to either arginine or glutamic acid . ^^^ Arginine mutations in all RFC genes tested permitted cell growth , although poor growth was observed for rfc 2 K71R . ^^^ A glutamic acid substitution resulted in lethality in RFC 2 and RFC 3 but not in RFC 1 or RFC 4 . ^^^ The rfc 2 K71R and rfc 4 K55R mutants show checkpoint defects , most likely in the intra S phase checkpoint . ^^^ These data demonstrate that the ATP binding function of RFC 2 is important for both DNA replication and checkpoint function and , for the first time , that RFC 4 also plays a role in checkpoint regulation . . ^^^ |
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| Interacting proteins: P35251 and P35250 |
Pubmed |
SVM Score :0.0 |
| Furthermore , the RFC ( 2 / 3 ) and RFC ( 3 / 4 ) subassemblies bind stably to PCNA , yet neither RFC 2 nor RFC 4 bind tightly to PCNA , indicating that RFC 3 forms a strong contact point to PCNA . ^^^ |
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| Interacting proteins: P35251 and P35250 |
Pubmed |
SVM Score :0.0 |
| Proteins required for this pathway include Rad 17 , Rad 9 , Hus 1 , Rad 1 , and Rfc 2 , Rfc 3 , Rfc 4 , and Rfc 5 . ^^^ |
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| Interacting proteins: P35251 and P35250 |
Pubmed |
SVM Score :0.0 |
| The ATP binding domains of Rfc 2 , Rfc 3 , and Rfc 4 are essential for DNA recognition and clamp loading . ^^^ The conserved lysine in the Walker A motif of the ATP binding domain encoded by the yeast RFC 1 , RFC 2 , RFC 3 , and RFC 4 genes was mutated to glutamic acid . ^^^ In contrast , complexes containing either a rfc 2 K71E mutation or a rfc 3 K59E mutation were severely impaired in ATPase and clamp loading activity . ^^^ Mutant RFC complexes containing rfc 2 K71R or rfc 3 K59R , carrying a conservative lysine > arginine mutation , had much milder clamp loading defects that could be partially ( rfc 2 K71R ) or completely ( rfc 3 K59R ) suppressed at high ATP concentrations . . ^^^ |
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| Interacting proteins: P35251 and P35250 |
Pubmed |
SVM Score :0.0 |
| Rad 24 is structurally related to subunits of the replication factor C ( RFC ) complex , and forms an RFC related complex with Rfc 2 , Rfc 3 , Rfc 4 , and Rfc 5 . ^^^ We also show that Chl 12 interacts physically with Rfc 2 , Rfc 3 , Rfc 4 , and Rfc 5 and forms an RFC related complex which is distinct from the RFC and RAD 24 complexes . ^^^ |
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| Interacting proteins: P35251 and P35250 |
Pubmed |
SVM Score :0.0 |
| BACKGROUND & OBJECTIVE : High expression of replication factor C ( RFC ) mRNA in hydatidiform mole and choriocarcinoma were detected previously with DNA microarray . ^^^ Replication factor C subunit 2 ( RFC 2 ) was reported to be associated with DNA duplication , DNA repair , and the function of cellular checkpoint . ^^^ Expression of RFC 2 and PCNA in different gestational trophoblastic diseases ] . ^^^ Replication factor C subunit 2 ( RFC 2 ) was reported to be associated with DNA duplication , DNA repair , and the function of cellular checkpoint . ^^^ The aim of current study was to investigate the expression levels of RFC 2 and proliferating cell nuclear antigen ( PCNA ) in gestational trophoblastic diseases ( GTD ) and to explore the relationship of expressions of two proteins with GTD . ^^^ |
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| Interacting proteins: P35251 and P35250 |
Pubmed |
SVM Score :0.0 |
| The DNA damage clamp loader replication factor C ( RFC Rad 24 ) consists of the Rad 24 protein and the four small Rfc 2 5 subunits of RFC . ^^^ In that system , three ATP molecules bound to the Rfc 2 , Rfc 3 , and Rfc 4 subunits are necessary and sufficient for efficient loading of PCNA , whereas ATP binding to Rfc 1 is not required . ^^^ |
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| Interacting proteins: P35251 and P35250 |
Pubmed |
SVM Score :0.0 |
| Loading of PCNA onto DNA at template primer junctions is performed in an ATP dependent process by replication factor C ( RFC ) , a heteropentameric AAA+ protein complex consisting of the Rfc 1 , Rfc 2 , Rfc 3 , Rfc 4 , and Rfc 5 subunits . ^^^ |
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| Interacting proteins: P35251 and P35250 |
Pubmed |
SVM Score :0.0 |
| The second subunit of the replication factor C complex ( RFC 40 ) and the regulatory subunit ( RIalpha ) of protein kinase A form a protein complex promoting cell survival . ^^^ RFC 40 , the second subunit of the RFC complex , and PCNA have been shown to be overexpressed in gestational trophoblastic diseases . ^^^ Using RFC 40 as the bait in a yeast two hybrid screening , we have identified a novel interaction between RFC 40 and the regulatory subunit ( RIalpha ) of cAMP dependent Protein kinase A ( PKA ) . ^^^ The interaction sites between these two proteins were investigated and mapped to the N terminus of RIalpha and the C terminus of RFC 40 . ^^^ Moreover , it was demonstrated that the C subunit of PKA was not associated with the RFC 40 RIalpha complex . ^^^ |
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| Interacting proteins: P35251 and P35250 |
Pubmed |
SVM Score :0.0 |
| RIalpha influences cellular proliferation in cancer cells by transporting RFC 40 into the nucleus . ^^^ We have recently identified a novel interaction between RIalpha and RFC 40 , the second subunit of Replication Factor C ( RFC ) , and have demonstrated that this interaction may be associated with cell survival . ^^^ Coincidentally , RFC 40 is overexpressed in gestational trophoblastic diseases such as choriocarcinomas . ^^^ Nuclear transport of RFC 40 was found to be dependent on RIalpha , and this transport appeared to be a crucial step for cell cycle progression from G 1 to S phase . ^^^ Impairment in the nuclear transport of RFC 40 by RIalpha arrested cells in G 1 phase . ^^^ |
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| Interacting proteins: P35251 and P35250 |
Pubmed |
SVM Score :0.0 |
| Cyclic AMP regulates the expression and nuclear translocation of RFC 40 in MCF 7 cells . ^^^ We have previously shown that the regulatory subunit of PKA , RIalpha , functions as a nuclear transport protein for the second subunit of the replication factor C complex , RFC 40 , and that this transport appears to be crucial for cell cycle progression from G 1 to S phase . ^^^ In this study , we found that N ( 6 ) monobutyryl cAMP significantly up regulates the expression of RFC 40 mRNA by 1 . 8 fold and its endogenous protein by 2 . 3 fold with a subsequent increase in the RIalpha RFC 40 complex formation by 3 . 2 fold . ^^^ Additionally , the nuclear to cytoplasmic ratio of RFC 40 increased by 26 % followed by a parallel increase in the percentage of S phase cells by 33 % . ^^^ Moreover , although cAMP did not affect the expression of the other RFC subunits , there was a significant decrease in the RFC 40 37 complex formation by 81 . 3 % , substantiating the decrease in DNA replication rate . ^^^ |
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| Interacting proteins: P35251 and P35250 |
Pubmed |
SVM Score :0.0 |
| Rfc 5 is positioned opposite the PCNA interface from Rfc 1 , and therefore , its action with Rfc 2 in opening PCNA indicates that PCNA is opened from the opposite side of the interface that the E . coli delta wrench acts upon . ^^^ Rfc 5 is positioned opposite the PCNA interface from Rfc 1 , and therefore , its action with Rfc 2 in opening PCNA indicates that PCNA is opened from the opposite side of the interface that the E . coli delta wrench acts upon . ^^^ |
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| Interacting proteins: P35251 and P35250 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P35251 and P35250 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P35251 and P35250 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P35251 and P35250 |
Pubmed |
SVM Score :0.0 |
| NA |
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| Interacting proteins: P35251 and P35250 |
Pubmed |
SVM Score :0.0 |
| NA |
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